KR20210143993A - Composition comprising dokdo aster oil - Google Patents

Abstract

The present invention relates to a composition for external application for skin containing oil of Aster sphathulifolius Maxim. (Dokdo aster) as an active ingredient. An objective of one embodiment of the present invention is to provide a composition for external application for skin which has effects of preventing skin wrinkles, improving skin elasticity, enhancing skin moisture, suppressing sebum secretion, alleviating troubles such as acne, and improving complexion, thereby protecting the skin from damage. Preferably, in the present invention, the Dokdo aster oil is prepared by extracting Dokdo aster three times by cold extraction at a temperature of 5℃, concentrating the same under reduced pressure at a temperature of 50℃ or less, and then aging the same at a low temperature of 4 to 20℃ for five to seven days. Preferably, the Dokdo aster oil is contained in an amount of 0.0001 to 0.5 wt% based on the total weight of the composition. The composition for external application for skin containing Dokdo aster oil as an active ingredient according to the present invention prevents skin wrinkles, improves skin elasticity, strengthens skin moisture, suppresses sebum secretion, relieves troubles such as acne, and improves complexion, thereby providing an excellent effect of delaying skin aging and protecting the skin from damage.

Description

Composition for external application for skin containing Dokdo Haeguk oil {COMPOSITION COMPRISING DOKDO ASTER OIL}

The present invention relates to a composition for external application for skin containing Dokdo Aster Oil as an active ingredient.

As used herein, “skin” refers to an organ covering the outside of an organism, and is composed of the epidermis, dermis, and subcutaneous fat layer, and includes not only the tissues covering the outside of the face or the entire body, but also the scalp and hair. am.

As used herein, "Dokdo Haeguk (scientific name Aster spathulifolius Maxim .; Dokdo Aster)" is a perennial plant belonging to the Asteraceae family, 30-60 cm tall, and the flowers are light purple, hanging one at the end of the branch, and have a diameter of 3.5-4 cm. The leaves are abundant, and there are many villi on both sides, and they grow alternately. It is usually planted as an ornamental plant and is also used for food and medicinal purposes. It is a native plant of Korea that grows on a sunny rocky or sloped place in Dokdo.

It is still unknown whether Dokdo Aster Oil has the effect of preventing skin wrinkles, improving skin elasticity, strengthening skin moisture, suppressing sebum secretion, relieving troubles such as acne, and improving complexion, and studies on this are insufficient.

The present inventors tested natural products for excellent skin protection and skin condition improvement on various plants, including Dokdo sea eagle and A region sea eagle. As a result, Dokdo Aster Oil prevents skin wrinkles, improves skin elasticity, and moisturizes skin. It was found to be excellent in strengthening, suppressing sebum secretion, alleviating troubles such as acne, and improving complexion.

It is an object of one embodiment of the present invention to provide a composition for external application for skin that has the effects of preventing skin wrinkles and improving skin elasticity, enhancing skin moisture, suppressing sebum secretion and alleviating troubles such as acne, and improving complexion, thereby protecting the skin from damage.

In order to achieve the above object, the present invention provides a composition for external application for skin containing Dokdo Aster Oil as an active ingredient.

Preferably, in the present invention, the Dokdo Aster Oil is concentrated under reduced pressure at a temperature of 50° C. or lower after extracting Dokdo Haguk three times by cold extraction at a temperature of 5° C., and then 4° C. to 20° C. It is aged at a low temperature for 5 to 7 days at a temperature of

Preferably, the Dokdo Aster Oil is contained in an amount of 0.0001 to 0.5% by weight based on the total weight of the composition.

The composition for external application for skin containing Dokdo Aster Oil as an active ingredient according to the present invention prevents skin wrinkles, improves skin elasticity, strengthens skin moisture, suppresses sebum secretion, and alleviates troubles such as acne, and improves complexion, resulting in skin aging It has an excellent effect of delaying skin irritation and protecting the skin from damage.

In order to obtain the desired effect in the present invention, the Dokdo Aster Oil is extracted from Dokdo Aster Oil three times using butylene glycol at a temperature of 5 ° C. It is concentrated under reduced pressure and aged at a low temperature for 5 to 7 days at a temperature of 4°C to 20°C.

There is also a method of volatilizing the solvent with a water bath at a temperature of 38°C using ethyl ether at room temperature and then performing vacuum steam distillation at a temperature of 30°C. and it is possible to obtain a higher quality extract in which the active ingredient is less denatured.

The cold extract may be extracted with a solvent selected from the group consisting of butylene glycol and propylene glycol, but is not limited thereto.

In order to obtain the desired effect in the present invention, the Dokdo Aster Oil is contained in an amount of 0.0001 to 0.5% by weight based on the total weight of the composition. If the content of the component is less than 0.0001% by weight, it is difficult to expect the action as an active ingredient, and if the content exceeds 0.5% by weight, there may be a problem in formulation stability.

Components included in the composition for external application for skin may include ingredients commonly used in compositions for external application for skin in addition to the active ingredient, for example, antioxidants, stabilizers, solubilizers, and conventional auxiliary agents such as vitamins, pigments and fragrances. .

The composition for external application for skin according to the present invention is not particularly limited in its formulation, for example, a cosmetic or lotion having a formulation of a softening lotion, a nutrient lotion, a massage cream, a nutrient cream, a pack, a gel, an essence, or a skin adhesion type cosmetic. , ointment, gel, cream, patch or spray can also be formulated as a cosmetic for transdermal administration.

In addition, in each formulation of the composition for external application for skin, other ingredients other than the above-mentioned essential ingredients can be selected and formulated by those skilled in the art without difficulty depending on the formulation or purpose of use of the other external preparation for skin.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples.

[Example 1]

300 g of Dokdo seaweed was picked, washed with purified water, dried, and then cut into 0.1~1cm pieces using a mixer. A solvent containing 700 ml of butylene glycol and 500 ml of water was added to the chopped Dokdo Haeguk, stirred and extracted for 5 days at a temperature of 5 ° C., and then filtered with Advantech No. 2 filter paper (Advantec No. 2, diameter 185 mm). A filtrate was obtained, and the obtained filtrate was extracted and filtered three times in the same manner as above, and then concentrated under reduced pressure at a temperature of 50° C. or less. The obtained extract was aged at a temperature of 4°C to 20°C for 5 to 7 days to obtain 11.45 g of a low-temperature aged extract.

[Example 2]

After taking 700 g of Dokdo seaweed and dividing it into 3 1L Erlenmeyer flasks, 1.5L of ethyl ether (Ethyl Ether, purity 99.0%, Fisher Scientific) was poured so that the seaweed was completely submerged and immersed for 4 hours. After filtering the sediment with Advantech No.2 filter paper, discard the seaweed, and for the extract, use 100g of anhydrous sodium sulfate (Na2SO4, Sodium Sulfate, purity 99.7%, Sigma Aldrich) to remove moisture, and then use Advantech No.2 filter paper again. so it was filtered. After volatilizing ethyl ether, a solvent, in a water bath at 38°C, 100 ml of distilled water was added to the residue, and steam distillation was performed under reduced pressure while maintaining the temperature at 30°C to obtain 13.0 mg of an extract.

When comparing the efficacy of the skin external composition containing each extracted Dokdo seagull essential oil as an active ingredient based on Examples 1 and 2 through Test Examples 1 to 6, the extraction efficiency when applying the method according to Example 1 was It was possible to obtain a higher quality extract that was better and less denatured of the active ingredient.

[Test Example 1] Wrinkle improvement effect measurement

In order to confirm the wrinkle improvement effect in humans by the composition of the present invention, a test example was prepared by using an external preparation as a nutritional cream formulation according to the composition ratio in Table 1. The unit of content ratio of the following compounding components is weight %.

Ingredients Preparation Example 1 Preparation 2 Comparative Example 1 Comparative Example 2 Purified water To 100 To 100 To 100 To 100 Dokdo Liberation Oil Refinery (Example 1) 0.1 - - - Dokdo Liberation Oil Refinery (Example 2) - 0.1 - - Haeguk refinery that grows wild in area A - - 0.1 - vegetable hydrogenated oil 1.50 1.50 1.50 1.50 stearic acid 0.60 0.60 0.60 0.60 glycerol stearate 1.00 1.00 1.00 1.00 stearyl alcohol 2.00 2.00 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 1.00 1.00 Arachinyl Behenyl Alcohol & Arachidyl Glucoside 1.00 1.00 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerol Oleate & Butylene Glycol 1.50 1.50 1.50 1.50 Caprylic/Carlic Triglycerides 11.00 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 6.00 preservative, fragrance appropriate amount appropriate amount appropriate amount appropriate amount triethanolamine 0.1 0.1 0.1 0.1

In order to confirm the wrinkle improvement effect of Preparation Example 1 and Preparation Example 2 and Comparative Example 1 and Comparative Example 2 of Table 1, evaluation was performed as follows. 60 healthy women in their 30s were divided into 4 groups of 15 people for 4 groups of Preparation Example 1, Preparation Example 2, Comparative Example 1, and Comparative Example 2, respectively, and the nourishing cream was applied to the face once a day for 8 weeks. A replica was made by using a dermatometer, and the state of the wrinkles was measured with a skin measuring instrument (visiometer, SV600, Courage+Khazaka electronic GmbH, Germany) and image analysis was performed. The results are shown in the table below. The values in the table below represent the average of each parameter value after 8 weeks of application minus the value of the variable before application.

Clinical results after 8 weeks of use R1 R2 R3 R4 R5 Preparation Example 1 -0.21 -0.21 -0.11 -0.04 -0.04 Preparation 2 -0.19 -0.20 -0.11 -0.03 -0.02 Comparative Example 1 -0.18 -0.19 -0.10 -0.02 -0.02 Comparative Example 2 0.26 0.25 0.22 0.03 0.03

R1: The difference between the highest and lowest values of the wrinkle contour line

R2: The average of the R1 values after dividing the wrinkle contour line by 5 arbitrarily

R3 : The highest value among R1 values divided by 5

R4: The average value obtained by subtracting the values of each peak and valley from the baseline of the wrinkle contour line

R5: The difference value of each wrinkle contour subtracted from the baseline of the wrinkle contour line

As shown in Table 2, it was found that the external composition of Preparation Example 1 had a relatively excellent skin wrinkle improvement effect. It can be seen that Preparation Example 1 containing Dokdo Aster Oil of the present invention has an excellent anti-wrinkle effect compared to the group coated with Preparation Example 2 as well as Comparative Examples 1 and 2.

[Test Example 2] Measurement of skin elasticity improvement effect

In order to confirm the skin elasticity improvement effect in humans by the composition of the present invention, Preparation Examples 1 and 2 and Comparative Examples 1 and 2 of Test Example 1 were evaluated as follows.

After dividing 60 healthy women in their 30s into 4 groups of 15 people for 2 groups of Comparative Example and Preparation Example, respectively, and applying the nourishing cream to the face twice daily for 8 weeks under the conditions of temperature 24-26°C and humidity of 75%, Skin elasticity was measured using a skin elasticity meter (Customer SEM 575, C+K Electronics Co., Germany). The results are shown in Table 3 below. The result values in Table 3 were described as ΔR8 (R8 (left)-R8 (right)) values of Customer SEM 575, and the R8 value represents the property of skin viscoelasticity.

Clinical results after 8 weeks of use skin elasticity effect Preparation Example 1 0.43 Preparation 2 0.42 Comparative Example 1 0.39 Comparative Example 2 0.11

As shown in Table 3, in Preparation Example 1 containing Dokdo Aster Oil of the present invention, skin elasticity was further increased compared to the group to which Preparation Example 2 was applied as well as Comparative Examples 1 and 2 it can be seen that

[Test Example 3] Measurement of skin moisturizing improvement effect

In order to confirm the skin moisturizing improvement effect in humans by the composition of the present invention, Preparation Examples 1 and 2 and Comparative Examples 1 and 2 of Test Example 1 were evaluated as follows.

60 healthy women in their 30s were divided into 4 groups of 15 for 4 groups of Preparation Example 1, Preparation Example 2 and Comparative Example 1 and Comparative Example 2, respectively, and applied a nourishing cream daily at a temperature of 24-26°C and a humidity of 75%. After allowing the face to be applied twice each for 4 weeks, before the start of application, at the time of 1, 2, and 4 weeks after application, and after 2 weeks of stopping (6 weeks in total) after application, constant temperature and constant humidity conditions (24 ℃, relative humidity 40%), the amount of skin moisture was measured with a coniometer. The results are shown in Table 4 below by expressing the increase in the measured value after treatment for a certain period of time based on the coniometer value measured just before the start of the test as a percentage.

Clinical Results Moisture increase rate (%) 1 week elapsed 2 weeks passed 4 weeks passed 6 weeks passed Preparation Example 1 34 41 42 32 Preparation 2 33 41 41 29 Comparative Example 1 33 40 41 27 Comparative Example 2 30 33 34 15

As shown in Table 4, Preparation Example 1 containing Dokdo Aster Oil of the present invention had a higher skin moisture retention than the group coated with Preparation Example 2 as well as Comparative Examples 1 and 2 confirmed to be increased. In addition, the value after 6 weeks of measuring the skin moisture after 2 weeks of stopping the application of the test substance appears similar to the value after 1 to 2 weeks, even if the test substance is not applied, the application of Preparation Example 1 to some extent It was found that the moisture retention of the skin was continuously maintained. Therefore, it can be seen that the Dokdo Aster Oil (Preparation Example 1) of the present invention enhances skin moisturizing.

[Test Example 4] Sebum secretion inhibitory effect

To measure the alleviation effect of oily skin, 40 women aged 20-45 years with oily skin were placed on the forehead, respectively, in Preparation Example 1, Preparation Example 2, Comparative Example 1, and Comparative Example 2 4 groups of 10 people each. After dividing into groups and applying twice a day in the morning and evening, 1 week, 2 weeks, 3 weeks, and 4 weeks later, the amount of sebum secretion of the skin was measured using a skin oil meter (Sebumeter SM810). The experiment was conducted at 20°C and 20% relative humidity, and the measurement results are shown in Table 5. The results are presented as the average value of 10 subjects. For oily skin, the measured value is 220 or more, and for normal skin, it is between 100 and 220.

test substance before application 1 week 2 weeks 3 weeks 4 weeks Preparation Example 1 246 237 230 216 212 Preparation 2 245 237 230 220 214 Comparative Example 1 246 238 232 225 217 Comparative Example 2 245 239 238 234 229

From the results of Table 5, Preparation Example 1 of Test Example 1 containing Dokdo Aster Oil of Examples according to the present invention produced excessive sebum secretion compared to Comparative Examples 1 and 2 as well as Preparation Example 2 It was confirmed that the sebum control effect was excellent by showing the effect of further suppressing the Therefore, Dokdo Aster Oil (Preparation Example 1) can also prevent acne and troubles caused by excessive sebum.

[Test Example 5] Effect of improving complexion of human skin (face)

In order to evaluate the effect of promoting blood circulation in the skin with respect to the composition containing Dokdo Aster Oil of the present invention, the degree of blood circulation in the skin was measured using a Laser Doppler Perfusion Imager (LDPI). LDPI is a device that is widely known and used today as a device to measure blood circulation in the skin. It is a very sensitive device that can measure not only the speed and amount of blood in the capillaries of the skin, but also the flow in arterioles and venules. .

After washing the face with soap in a constant temperature and humidity room, the face was acclimatized for 30 minutes, and the initial value was measured using LDPI and an IR (Infrad) camera, which is a skin temperature measuring device. The number of participants in the experiment was 40 women with cold hands and feet. Initial blood flow was measured under the forehead with LDPI, and initial values of skin temperature on the forehead, under the eyes and cheeks were measured using an IR camera.

For each of the four groups of Preparation Example 1 and Preparation Example 2 and Comparative Example 1 and Comparative Example 2 of Test Example 1, 10 people were divided into 4 groups, and the subjects were allowed to use them for 1 week, and then the average value was taken to determine the blood flow and skin temperature. The measured values were compared, and the results are shown in Tables 6 and 7 below, respectively.

Face before use 1 week after use Preparation Example 1 0.939 1.155 Preparation 2 0.939 1.150 Comparative Example 1 0.940 1.009 Comparative Example 2 0.939 0.958

forehead under the snow cheek Preparation Example 1 before use 32.20 32.18 30.30 1 week after use 33.59 33.69 33.81 Preparation 2 before use 32.21 32.19 30.33 1 week after use 33.53 33.61 33.76 Comparative Example 1 before use 32.20 32.19 30.32 1 week after use 33.16 33.17 33.05 Comparative Example 2 before use 32.22 32.20 30.32 1 week after use 32.74 32.92 31.19

As described above, as a result of using Preparation Example 1 containing Dokdo Aster Oil according to the present invention, the effect of improving peripheral blood circulation in the faces of test subjects compared to Preparation Example 2 as well as Comparative Examples 1 and 2 Due to this, it could be confirmed that the complexion was further improved, which ultimately delivered the nutrients of the skin most effectively compared to other groups when the Dokdo Aster Oil (Preparation Example 1) according to the present invention was contained and reduced skin aging. suggest that it may contribute to suppression and delay.

[Test Example 6] Acne and skin trouble improvement effect

The number of participants in the experiment was 40 women who usually had severe skin problems including acne, washed their faces with soap in a constant temperature and humidity room, then acclimatized them for 30 minutes, and compared the number of acne and the number with inflammation to measure the degree of skin trouble. .

The test subjects were allowed to use Preparation Example 1 and Preparation Example 2 and Comparative Example 1 and Comparative Example 2 of Test Example 1 for 4 weeks, respectively, and then compared the degree of acne and skin troubles, and the results are shown in Table 8 below. It was.

Production Example 1 (10 people) Production Example 2 (10 people) Comparative Example 1 (10 people) Comparative Example 2 (10 people) Skin trouble improvement rate 83% 77% 72% 38%

As shown in Table 8 above, as a result of applying Preparation Example 1 containing Dokdo Aster Oil of Test Example 1 according to the present invention for 4 weeks, the reduction in the number of existing acne and the improvement of the troubles accompanied by inflammation were different. It was found to be relatively superior to that of the groups.

Hereinafter, based on Example 1 above, a formulation example of the composition for external application for skin containing Dokdo Aster Oil according to the present invention will be described in more detail.

[Formulation Example 1] Softening lotion (skin lotion)

Ingredients weight% Purified water To 100 Dokdo Liberation Oil Refinery (Example 1) 0.1 butylene glycol 2.0 propylene glycol 2.0 Carboxyvinyl Polymer 0.1 PEG-12 Nonylphenyl Ether 0.2 Polysorbate 80 0.4 ethanol 10.0 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount

[Formulation Example 2] Nutrient lotion (milk lotion)

Ingredients weight% Purified water To 100 Dokdo Liberation Oil Refinery (Example 1) 0.1 beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 liquid paraffin 0.5 Caprylic/Capric Triglycerides 5.0 glycerin 3.0 butylene glycol 3.0 propylene glycol 3.0 Carboxyvinyl Polymer 0.1 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount

[Formulation Example 3] Nourishing cream

Ingredients weight% Purified water To 100 Dokdo Liberation Oil Refinery (Example 1) 0.1 beeswax 10.0 Polysorbate 60 1.5 sebum 60 hydrogenated castor oil 2.0 Sorbitan sesquioleate 0.5 liquid paraffin 10.0 squalane 5.0 Caprylic/Capric Triglycerides 5.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount

[Formulation Example 4] Massage Cream

Ingredients weight% Purified water To 100 Dokdo Liberation Oil Refinery (Example 1) 0.1 beeswax 10.0 Polysorbate 60 1.5 sebum 60 hydrogenated castor oil 2.0 Sorbitan sesquioleate 0.8 liquid paraffin 40.0 squalane 5.0 Caprylic/Capric Triglycerides 4.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount

[Formulation Example 5] Pack

Ingredients weight% Purified water To 100 Dokdo Liberation Oil Refinery (Example 1) 0.1 polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 allantoin 0.1 ethanol 6.0 PEG-12 Nonylphenyl Ether 0.3

Claims (6)

A composition for external application for skin containing Dokdo Haeguk oil as an active ingredient. The method of claim 1, wherein the Dokdo haeguk oil is concentrated under reduced pressure at a temperature of 50 ℃ or less after extracting Dokdo haeguk three times at a temperature of 5 ℃, and aged at a low temperature for 5 to 7 days at a temperature of 4 ℃ to 20 ℃. A composition for external application to the skin, characterized in that it is an extract. [Claim 3] The composition for external application for skin according to any one of claims 1 to 2, wherein the composition for external application for skin is used for preventing skin wrinkles and improving skin elasticity. The composition for external application for skin according to any one of claims 1 to 2, wherein the composition for external application for skin has a skin moisturizing purpose. [Claim 3] The composition for external application for skin according to any one of claims 1 to 2, wherein the composition for external application for skin is used for controlling sebum of the skin and preventing or improving skin troubles including acne. The composition for external application for skin according to any one of claims 1 to 2, wherein the composition for external application for skin has a use for improving skin complexion.