KR20230090394A - Cosmetic composition for preventing hair loss or promoting hair growth with plant extract as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for preventing hair loss or promoting hair growth containing, as active ingredients, extracts of Sophora flavescens, Phellodendron amurense, Scutellaria baicalensis, Liriope muscari and Dictamnus dasycarpus. Since the present invention shows an excellent dermal papilla cell proliferation effect, the functionality of alleviation, prevention, or treatment of hair loss can be usefully used in the fields of cosmetics, health functional foods, quasi-drugs, or pharmaceuticals.

Description

Cosmetic composition for preventing hair loss or promoting hair growth with plant extract as an active ingredient}

The present invention relates to a cosmetic composition for preventing hair loss or promoting hair growth comprising a plant extract as an active ingredient.

Hair protects the human body from external stimuli, such as protecting the scalp and maintaining body temperature. Human hair is generally about 100,000 to 150,000, and is formed in the hair follicle. Each hair grows and falls out through different cycles (anagen phase, catagen phase, and telogen phase). This cycle is repeated over 3 to 6 years, and an average of 50 to 100 hairs per day fall out normally.

The cycle of hair is modified and shortened by internal and external factors such as hormones, stress, disease, environmental pollution, and smoking. change will appear. Alopecia refers to an abnormally large number of hairs falling out due to a shortened growth period hair ratio and an increase in catagen or telogen hairs during this cycle, and hair loss symptoms can be suspected when more than 100 hairs fall out.

Minoxidil and finasteride, which are representative hair growth agents that are currently known for their efficacy and are widely used worldwide, require continuous use throughout a lifetime to maintain the effect of hair growth and hair loss prevention. is currently limited.

Recently, efforts to develop skin functional cosmetic compositions using safe natural plant extracts from skin irritation have continued, and a method for separating and purifying cosmetically active ingredients including p-coumaric acid from Jeju bamboo shoots (Korean Patent Publication No. 10-2012 -0037279), antioxidant, anti-wrinkle and moisturizing improvement functional cosmetic composition containing rice water fermented culture as an active ingredient and its manufacturing method (Korean Patent Publication No. 10-2016-0075576), and root fermentation of ginseng grown in cold air Techniques such as an outpost extract containing water and a cosmetic composition containing the same (Korean Patent Publication No. 10-2019-0086220) have been disclosed. However, in the case of using specific 1-2 plant extracts as the main raw materials as described above, it is difficult to secure a large amount of specific components, and there is a problem of causing toxicity or tolerance.

Therefore, the present invention has confirmed the effect of preventing hair loss or promoting hair growth using plant extracts with few side effects, which can be used as a cosmetic composition, a pharmaceutical composition, a quasi-drug composition, or a health functional food composition.

KR 10-1973807 B1

The present invention is to provide a composition showing an excellent effect in preventing hair loss or promoting hair growth as it contains a sorghum ginseng extract, a hwangbaek extract, a golden extract, a macmundong extract, and an extract of baekseonpi. The composition may be used as a cosmetic composition, a pharmaceutical composition, a quasi-drug composition or a health functional food composition.

The present invention provides a cosmetic composition for preventing hair loss or stimulating hair growth, comprising a sorghum ginseng extract, a hwangbaek extract, a golden extract, an extract of ginseng chinensis, and an extract of ringworm skin as active ingredients.

In the present invention, the sorghum ginseng extract, the yellow baek extract, the golden extract, the macmundong extract, and the baekseonpi extract may be preferably extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

In the present invention, the ginseng extract, the hwangbaek extract, the golden extract, the macmundong extract, and the baekseonpi extract are preferably mixed in a weight ratio of 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2 It is good.

In the present invention, the high ginseng extract, hwangbaek extract, golden extract, lycopene extract and ringworm extract are preferably used to proliferate dermal papilla cells.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of hair loss comprising a sorghum ginseng extract, a hwangbaek extract, a golden extract, a wiltong extract, and an extract of baekseonpi as an active ingredient.

In addition, the present invention provides a quasi-drug composition for preventing hair loss or stimulating hair growth, containing as active ingredients an extract of sorghum ginseng, an extract of hwangbaek, a golden extract, an extract of rhubarb, and an extract of baekseonpi.

In addition, the present invention provides a health functional food composition for preventing hair loss or stimulating hair growth, containing as active ingredients an extract of sorghum ginseng, a hwangbaek extract, a golden extract, an extract of Macmundong, and an extract of Baekseonpi.

The present invention provides a composition having an effect of preventing hair loss or promoting hair growth as it contains as active ingredients a sophora ginseng extract, a hwangbaek extract, a golden extract, a rhubarb extract, and an extract of baekseonpi. Kosam extract, hwangbaek extract, golden extract, rhubarb extract, and algae extract of the present invention exert an effect of preventing hair loss and promoting hair growth by proliferating dermal papilla cells.

1 to 3 are diagrams showing the dermal papilla cell proliferation effect according to the mixture treatment of Kosam extract, hwangbaek extract, golden extract, macmundong extract and ringworm extract in one embodiment of the present invention.

The present invention provides a cosmetic composition for preventing hair loss or stimulating hair growth, comprising a sorghum ginseng extract, a hwangbaek extract, a golden extract, an extract of ginseng chinensis, and an extract of ringworm skin as active ingredients.

Gosam ( Sophora flavescens ) is a leguminous plant of rosewood, and in oriental medicine, dried roots are called gosam. It has a bitter taste and is said to be effective for indigestion and neuralgia.

Hwangbaek is a dried bark of Phelledendron amurense belonging to the Rutaceae family, and is known to have blood sugar lowering, sterilization effects, blood pressure lowering, and anti-inflammatory effects.

Golden ( Scutellaria baicalensis ) is a plant belonging to the Lamiaceae thimble flower genus. In oriental medicine, the golden root is used as an antipyretic, diuretic, antidiarrheal, choleretic, etc. In addition, anti-inflammatory, anti-allergic, antibacterial, and antiviral effects are known.

Macmundong ( Liriope platyphylla ) is a plant of the Liliaceae family, and in oriental medicine, the tuberous root is used as a medicine.

Ringworm is the root bark of ringworm ( Dictamnus dasycarpus ), which is effective for skin rashes, eczema, rubella, itching, allergic dermatitis, etc., and is known to have antipyretic action and skin fungal inhibitory action.

In the present invention, the ginseng extract, the hwangbaek extract, the golden extract, the macmundong extract, and the baekseonpi extract are preferably mixed in a weight ratio of 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2 It is good, and accordingly, by proliferating dermal papilla cells, it shows an effect of preventing hair loss or promoting hair growth.

In addition, the present invention provides a pharmaceutical composition for preventing or treating hair loss, a quasi-drug composition, and a health functional food composition comprising a sorghum ginseng extract, a hwangbaek extract, a golden extract, a rhubarb extract, and an extract of baekseonpi as active ingredients.

Throughout this specification, '%' used to indicate the concentration of a particular substance is solid/solid (w/w) %, solid/liquid (w/v) %, and Liquid/liquid is (v/v) %.

In the present invention, the extract may be obtained by extraction and separation from nature using an extraction and separation method known in the art, and the "extract" defined in the present invention is extracted using an appropriate solvent. , For example, a crude extract, a polar solvent-soluble extract, or a non-polar solvent-soluble extract are all included. As a suitable solvent for extracting the extract, any food science or pharmaceutically acceptable organic solvent may be used, and water or an organic solvent may be used, but is not limited thereto. For example, purified water, methanol ), alcohols having 1 to 4 carbon atoms including ethanol, propanol, isopropanol, butanol, butylene glycol, etc., acetone, ether, Various solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane may be used alone or in combination. As an extraction method, any one of methods such as hot water extraction, cold brew extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be selected and used. In addition, the desired extract may be additionally subjected to a conventional fractionation process or may be purified using a conventional purification method.

There is no limitation on the preparation method of the extract of the present invention, and any known method may be used. For example, the extract included in the composition of the present invention can be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying of the primary extract extracted by the above-described hot water extraction or solvent extraction method. In addition, a fraction further purified from the primary extract using various chromatography such as silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography you can also get Therefore, in the present invention, the extract is a concept that includes all extracts, fractions, and purified products obtained in each step of extraction, fractionation, or purification, and dilutions, concentrates, or dried products thereof. The extract of the present invention can be preferably extracted using a 20-40% (v/v) aqueous solution of butylene glycol, and more preferably, water and butylene glycol are mixed at a volume ratio of 7:3. It is recommended to extract at 40 to 60 ℃ for 60 to 84 hours using a 30% (v / v) aqueous solution of butylene glycol.

The "cosmetic composition" of the present invention may be prepared by including a cosmetically effective amount of the extract of the present invention described above and a cosmetically acceptable carrier.

In the present specification, "a cosmetically effective amount" means an amount sufficient to achieve the efficacy of preventing hair loss or promoting hair growth through the proliferation of dermal papilla cells of the composition of the present invention described above.

The appearance of the cosmetic composition contains a cosmetic or dermatologically acceptable medium or base. These are all formulations suitable for topical application, e.g. solutions, gels, solids, anhydrous pasty products, emulsions obtained by dispersing an oily phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or ionic forms (liposomes) and It may be provided in the form of a non-ionic follicular dispersant, or in the form of a cream, toner, lotion, powder, ointment, spray or conceal stick. These compositions can be prepared according to conventional methods in the art. The composition according to the present invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its dosage form, and is, for example, hair serum, hair tonic, hair nourishing lotion, hair treatment, hair shampoo, hair rinse, scalp and hair combination treatment. It can be formulated as a cosmetic such as a ment, but it is not limited to the above formulation as it can be commonly used in the field of cosmetics for scalp and hair, and is appropriately selected without difficulty by a person skilled in the art according to the type or purpose of use of other external agents can be combined.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fibers, vegetable fibers, wax, paraffin, starch, tracanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. this can be used

When the formulation of the cosmetic composition of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additionally chlorofluorohydro propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate , fatty acid amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters, and the like can be used.

In addition to the extract of the present invention, the cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extracts.

In addition to the above functional additives, the cosmetic composition of the present invention may further contain components included in general cosmetic compositions as needed. Ingredients other than those included include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation accelerators, cooling agents, antiperspirants, purified water and the like.

The quasi-drug composition of the present invention refers to items that are less active than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, mitigating, treating or preventing human or animal diseases. For example, according to the Pharmaceutical Affairs Act, quasi-drugs are pharmaceuticals. Textile/rubber products used for the treatment or prevention of human/animal diseases, non-tools or machines that have little or no direct action on the human body, and similar products to prevent infectious diseases This includes disinfectants and insecticides for The type or formulation of the quasi-drug composition of the present invention is not particularly limited, but is preferably a composition for scalp and hair care, more preferably a composition for preventing hair loss or promoting hair growth.

As used herein, the term “scalp and hair care composition” refers to a composition that prevents or alleviates damage to the scalp and hair, and “composition for preventing hair loss or promoting hair growth” refers to a composition that prevents hair from falling out or prevents hair from forming or Means a composition that promotes growth. The formulation of the composition may be shampoo, rinse, essence, nutrient, serum, massage cream, lotion, pack, etc., but is not limited thereto.

The quasi-drug composition of the present invention may further include adjuvants commonly used in cosmetic compositions within a range that does not impair the effects of the present invention. The type of adjuvant that can be used in the present invention is not particularly limited, and any adjuvant commonly used in this technical field may be used. The adjuvants may include, for example, conventional adjuvants such as gelling agents, activators, preservatives, fillers, antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors.

When the mixture of the present invention is added to a quasi-drug composition for the purpose of preventing hair loss or promoting hair growth, the extract may be added as it is or used together with other quasi-drug ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient can be suitably determined depending on the purpose of use.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent as needed in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, aromatics, preservatives, and the like. can include

On the other hand, the pharmaceutical composition, quasi-drug composition or cosmetic composition of the present invention can be applied to any part of the body requiring hair growth as well as the scalp. For example, it can also be used for areas where hair or hair is damaged due to scars caused by trauma, or for improving conditions of wide forehead or M-shaped forehead, eyelashes or eyebrows, and alopecia for the purpose of simple cosmetic effect.

The health functional food composition of the present invention may contain various flavoring agents or natural carbohydrates as additional ingredients, like conventional food compositions, in addition to containing an extract as an active ingredient.

Examples of the aforementioned natural carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrins, cyclodextrins, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agents described above, natural flavoring agents (thaumatin), stevia extracts (eg rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can advantageously be used. The food composition of the present invention is formulated in the same way as the following pharmaceutical composition and can be used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, chewing gum, candy, ice cream, alcoholic beverages, vitamin complexes and health supplements, etc. there is

In addition, the food composition includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and salts thereof, in addition to extracts that are active ingredients. , alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like. In addition, the food composition of the present invention may contain natural fruit juice and fruit flesh for preparing fruit juice beverages and vegetable beverages.

The health functional food composition of the present invention may be prepared and processed in the form of tablets, capsules, powders, granules, liquids, pills, and the like. In the present invention, 'health functional food composition' refers to a food manufactured and processed using raw materials or ingredients having useful functionalities for the human body according to the Health Functional Food Act No. 6727, and the structure and function of the human body It refers to intake for the purpose of obtaining useful effects for health purposes such as regulating nutrients or physiological functions. The health functional food of the present invention may contain ordinary food additives, and the suitability as a food additive is determined according to the general rules of the Food Additive Code and general test methods approved by the Ministry of Food and Drug Safety, unless otherwise specified. It is judged according to the relevant standards and standards. Examples of the items listed in the 'Food Additive Code' include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; natural additives such as persimmon pigment, licorice extract, crystalline cellulose, kaoliang pigment, and guar gum; and mixed preparations such as sodium L-glutamate preparations, noodle-added alkali preparations, preservative preparations, and tar color preparations. For example, a health functional food in the form of a tablet is obtained by granulating a mixture obtained by mixing the active ingredient of the present invention with an excipient, a binder, a disintegrant, and other additives in a conventional manner, and then adding a lubricant or the like to compression molding, or The mixture can be directly compression molded. In addition, the health functional food in the form of a tablet may contain a flavoring agent or the like, if necessary. Among capsule-type health functional foods, hard capsules can be prepared by filling a mixture in which the active ingredient of the present invention is mixed with additives such as excipients in a normal hard capsule. It can be prepared by filling a mixture mixed with gelatin in a capsule base. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary. The health functional food in the form of a pill can be prepared by molding a mixture of the active ingredient of the present invention mixed with an excipient, binder, disintegrant, etc. by a conventionally known method, and can be coated with sucrose or other coating agent if necessary, Alternatively, the surface may be coated with a material such as starch or talc. Health functional food in the form of granules can be prepared in granular form by a conventionally known method of mixing the excipients, binders, disintegrants, etc. of the active ingredients of the present invention, and if necessary, flavoring agents, flavoring agents, etc. can

In addition, in the present invention, the pharmaceutical composition may further include an adjuvant in addition to the active ingredient. Any of the adjuvants known in the art may be used without limitation.

The pharmaceutical composition according to the present invention may be prepared in the form of incorporating the active ingredient into a pharmaceutically acceptable carrier. Here, the pharmaceutically acceptable carrier includes carriers, excipients and diluents commonly used in the pharmaceutical field. Pharmaceutically acceptable carriers usable in the pharmaceutical composition of the present invention include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical composition of the present invention may be formulated and used in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories or sterile injection solutions according to conventional methods, respectively. .

When formulated, it may be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations contain at least one or more excipients such as starch, calcium carbonate, sucrose, lactose, and gelatin in addition to active ingredients. It can be prepared by mixing etc. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration include suspensions, solutions for oral administration, emulsions, syrups, etc. In addition to commonly used diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. can Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for suppositories, Witepsol, Tween 61, cacao paper, laurin paper, glycerogelatin, and the like may be used.

The pharmaceutical composition according to the present invention can be administered to a subject by various routes. All modes of administration are contemplated, eg oral, intravenous, intramuscular, subcutaneous, intraperitoneal injection.

The pharmaceutical composition may be formulated into various oral or parenteral dosage forms.

Formulations for oral administration include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, granules, etc. chlorose, mannitol, sorbitol, cellulose and/or glycine), lubricants such as silica, talc, stearic acid and magnesium or calcium salts thereof and/or polyethylene glycol. In addition, the tablet may contain a binder such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and/or polyvinylpyrrolidine, and in some cases starch, agar, alginic acid or a disintegrant or effervescent mixture, such as its sodium salt, and/or absorbents, colorants, flavors, and sweeteners. The formulation may be prepared by conventional mixing, granulating or coating methods.

In addition, a typical formulation for parenteral administration is an injection formulation, and water, Ringer's solution, isotonic physiological saline or suspension may be used as a solvent for the injection formulation. Sterile fixed oils of the above injectable preparations may be used as a solvent or suspension medium, and any bland fixed oil may be used for this purpose, including mono- and di-glycerides.

In addition, the formulation for injection may use a fatty acid such as oleic acid.

Hereinafter, the present invention will be described in more detail through examples and experimental examples. However, the following examples and experimental examples are presented as examples of the present invention, and if it is determined that detailed descriptions of well-known techniques or configurations may unnecessarily obscure the gist of the present invention, detailed descriptions thereof will be omitted. It can be done, and the present invention is not limited thereby. Various modifications and applications of the present invention are possible within the scope of the claims described below and equivalents interpreted therefrom.

<Preparation Example 1> Preparation of plant extract

After adding 30% (v/v) of butylene glycol 10 times the weight of each of red ginseng, hwangbaek, golden, macmundong, and baekseonpi, heat and stir at 50 ° C for 72 hours, and filter the filter under reduced pressure for the first time to obtain each extract. was manufactured. The filtered extract was refrigerated at a temperature of 4°C for 24 hours, filtered again under reduced pressure for the second time, and then stored in a refrigerator at a temperature of 4°C.

<Preparation Example 2> Preparation of cosmetic composition

The extract prepared in Preparation Example 1 was weighed by weight as shown in Table 1 below, mixed and stirred, and then filtered under reduced pressure for the third time to prepare a mixture, which was used in the following experiments. As shown in Table 1, a case in which no extract was included was used as a control group.

gosam yellow white Gold Mcmun-dong ringworm Example 1 200g 100 grams 100 grams 100 grams 100 grams Example 2 100 grams 100 grams 100 grams 100 grams 100 grams Example 3 200g 100 grams 200g 100 grams 100 grams control group - - - - -

<Experimental Example 1> Confirmation of dermal papilla cell proliferation effect

In this experiment, the dermal papilla cell proliferation effect of the mixture prepared in Preparation Example 2 was confirmed. Hair roots are protected by hair follicles, and hair cells surround Human Dermal Papilla cells (HDP cells) located at the bottom of the hair roots. Dermal papilla cells play a role in growing hair by receiving oxygen and nutrients from capillaries, and are important cells that determine the number, thickness, and cycle of new hair growth. On the other hand, when dermal papilla cells are stimulated, they destroy hair follicles and block the secretion of growth factors that grow hair, causing hair loss.

The proliferation efficiency of dermal papilla cells, which play an important role in hair formation, was evaluated by the WST-8 test method. HDP cells were dispensed at 1.2 × 10 ⁴ cells/200 μl per well in a 96 well plate with DMEM containing 10% FBS, and then cultured for 24 hours in a 37° C., 5% CO ₂ incubator. After removing all the cell supernatant, the mixture diluted in DMEM (Serum free media, SFM) without FBS (Examples 1 to 3) and untreated (control) were repeated three times at 200 μl per well, respectively. After treatment, it was cultured for 24 hours in a 37°C, 5% CO ₂ incubator. In addition to the medium containing the sample, 10 μl of CCK-8 reagent was added per well and reacted in an incubator for 3 hours. Absorbance was measured at a wavelength of 450 nm. The absorbance value of each sample was expressed as a percentage according to Equation 1 below.

The dermal papilla cell proliferation effect of the mixture according to each mixing ratio is shown in FIGS. 1 to 3. As shown in Figure 1, when the mixture of Example 1 of the present invention was treated with 0.5 to 2% (v / v), it was confirmed that the dermal papilla cell proliferation effect was 23 to 39.1% compared to the control group. In particular, in the case of the mixture of Example 1, it was found that the dermal papilla cell proliferation effect increased according to the treatment concentration, and the 2% (v / v) treatment showed the highest dermal papilla cell proliferation effect at 39.1% compared to the control group. On the other hand, the Example 2 mixture showed 26.4% of dermal papilla cell proliferation compared to the control group when treated with 1% (v / v), and the Example 3 mixture showed 20.8% of dermal papilla cell proliferation compared to the control group when treated with 0.5% (v / v). Show the effect, it was confirmed that the dermal papilla cell proliferation efficacy appears differently depending on the compounding ratio of each extract.

Claims (7)

A cosmetic composition for preventing hair loss or stimulating hair growth, comprising ginseng extract, hwangbaek extract, golden extract, rhubarb extract, and ringworm extract as active ingredients.
According to claim 1,
The high ginseng extract, yellow baek extract, golden extract, macmundong extract and ringworm extract,
A cosmetic composition for preventing hair loss or promoting hair growth, characterized in that it is extracted with water, alcohol having 1 to 4 carbon atoms or a mixed solvent thereof.
According to claim 1,
The high ginseng extract, yellow baek extract, golden extract, macmundong extract and ringworm extract,
A cosmetic composition for preventing hair loss or promoting hair growth, characterized in that it is mixed in a weight ratio of 1 to 2: 1 to 2: 1 to 2: 1 to 2: 1 to 2.
According to claim 1,
The high ginseng extract, yellow baek extract, golden extract, macmundong extract and ringworm extract,
A cosmetic composition for preventing hair loss or promoting hair growth, characterized in that for proliferating dermal papilla cells.
A pharmaceutical composition for the prevention or treatment of hair loss comprising a sorghum ginseng extract, a hwangbaek extract, a golden extract, a rhubarb extract, and an extract of baekseonpi as an active ingredient.
A quasi-drug composition for preventing hair loss or stimulating hair growth, containing as active ingredients an extract of sorghum ginseng, a hwangbaek extract, a golden extract, an extract of rhubarb, and an extract of ringworm.
A health functional food composition for preventing hair loss or promoting hair growth, comprising ginseng extract, hwangbaek extract, golden extract, maekmundong extract, and ringworm extract as active ingredients.

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