KR101846503B1 - Composition for Preventing Hair Loss or Improving Hair Growth Hibiscus and Pawwpaw - Google Patents

Abstract

The present invention provides a composition for preventing hair loss or improving hair growth, comprising Hibiscus and pawpaw. The composition according to the present invention has effects of proliferating the dermal papilla cells of a rat through synergy of Hibiscus and pawpaw, inhibiting the activity of 5α-reductase, and growing the length of a hair fiber of rat hair follicles. Therefore, the composition can be useful as a raw material for a functional cosmetic composition or a pharmaceutical composition for preventing hair loss and improving hair growth. The components can be eaten as natural materials, and thus the composition of the present invention including the same as active ingredients is safe even when being used for a long time.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing hair loss or improving hair growth,

The present invention relates to a composition for the prevention of hair loss and hair loss of papaya and its effect and its efficacy.

Hair loss occurs in many men and women and causes emotional distress. Hair loss has been found to have features such as an increase in vellus hair, a reduction in follicles, and a decrease in growing follicles. Androgenetic alopecia (AGA) is the most common form of hair loss occurring in men over 40 years of age. Although the study on the development of therapeutic agents and the mechanism of the increase of the hair loss population is actively carried out, it is not known exactly what causes the hair loss.

Drugs that promote hair growth are now approved by the US Food and Drug Administration (FDA) and are well known for finasteride and minoxidil.

Finasteride has been developed as a therapeutic agent for the treatment of benign prostatic hyperplasia, a substance that inhibits the activity of type Ⅱ 5α-reductase, and is known to promote hair growth in patients with androgenetic alopecia.

Minoxidil has been developed as a vasodilator for the treatment of hypertension, but is reported to be a side effect and has been used as a hair growth agent. Although the mechanism of action of minoxidil on hair growth has not been clarified to date, it is known that KATP-channel oppening effect, increase of nutritional supply through vasodilation and activation of β-catenin pathway in dermal papilla cell, It is thought to induce growth. It is also known that minoxidil proliferates dermal papilla cells through decreased apoptosis of dermal papilla cells.

Dermal papilla cells are mesenchymal derived fibroblasts that secrete proteins such as insulin-like growth factor-1 (IGF-1), hepatocyte growth factor (HGF) and fibroblast growth factor-7 (FGF-7) It has been reported to affect the hair cycle.

Studies on hair growth in terrestrial plants and seaweeds have been actively conducted, but there is almost no commercial usefulness.

Prior Art 1 relates to a hair growth inhibitor composition containing a seaweed extract in addition to a ginseng extract or a pepper extract, and the prior art 2 relates to a hair growth promoter containing an active ingredient extracted from a seaweed, a mallow, a seaweed, etc. by utilizing a low temperature nano extract method.

However, the inventions of the above two references do not contain any gold leaf extracts at all and do not contain any hair growth promoting effects of gold leaf.

Patent Document 1: Korean Patent Registration No. 0668878, hair growth composition, composition for external application for hair growth and composition for hair growth, composition for external application for hair growth Patent Document 2: Korean Patent Registration No. 0908745, hair growth promoting agent using low temperature nano extract method and production method thereof

The inventors of the present invention have continuously studied on the effect of preventing hair loss and improving hair growth, and as a result, it has been found that a composition comprising an active ingredient together with a gold coin and a cell product proliferates a dermal papilla cell of a rat, Of the present invention has the effect of inhibiting the activity of the hair follicle of the present invention and of growing the hair-fiber length of the rat vibrissa follicles, thereby completing the present invention.

According to an aspect of the present invention,

Gold powder, gold powder, gold powder, and a mixture thereof; And

The present invention provides a hair loss preventing or hair growth improving composition comprising, as an active ingredient, any one selected from the group consisting of papaya pulp powder, papaya leaf powder, papaya pulp extract, papaya leaf extract and mixtures thereof.

In the hair loss preventing or hair growth improving composition according to the present invention,

Wherein the gold powder is one selected from the group consisting of gold leaf powder, gold leaf powder, gold leaf powder and gold powder, and mixtures thereof,

It is preferable that the gold leaf extract is any one selected from the group consisting of gold leaf, leaf extract, gold leaf powder, gold leaf root extract, gold leaf extract, and mixtures thereof.

In the hair loss preventing or hair growth improving composition according to the present invention,

The extract may be extracted with an extraction solvent selected from the group consisting of water, a C1 to C4 alcohol, hexane, ethyl acetate, butylene glycol, propylene glycol, glycerin, acetic acid, ether, chloroform and a mixed solvent thereof .

In the hair loss preventing or hair growth improving composition according to the present invention,

0.01 to 70% by weight of any one selected from the group consisting of gold powder, gold powder,

It is preferable that 0.01 to 70% by weight of any one selected from the group consisting of papaya pulp powder, papaya leaf powder, papaya pulp extract, papaya leaf extract and mixtures thereof is contained.

The hair loss preventing or hair growth improving composition according to the present invention is useful for proliferating dermal papilla cells of rats, inhibiting the activity of 5α-reductase and inhibiting the activity of rat moist hair follicles And has the effect of growing hair-fiber length. Therefore, the composition exhibits a useful effect for preventing hair loss and / or improving hair growth. Especially, the gold leaf and papaya can be edible as natural materials, and therefore, the composition of the present invention containing it as an effective ingredient has a safety advantage for long-term use.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention. The contents of all publications referred to herein are incorporated herein by reference.

The term "about" is used herein to refer to a reference quantity, a level, a value, a number, a frequency, a percent, a dimension, a size, a quantity, a weight, or a length of 30, 25, 20, 25, 10, 9, 8, 7, Level, value, number, frequency, percent, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words "comprises" and "comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

Hereinafter, the present invention will be described in detail.

The present inventors have succeeded in using an in vitro method to proliferate a dermal papilla cell of a rat, inhibit the activity of 5α-reductase, and inhibit the development of rat vibrissa follicles, And the hair-fiber length of the hair-fiber.

Therefore,

Gold powder, gold powder, gold powder, and a mixture thereof; And

The present invention provides a hair loss preventing or hair growth improving composition comprising, as an active ingredient, any one selected from the group consisting of papaya pulp powder, papaya leaf powder, papaya pulp extract, papaya leaf extract and mixtures thereof.

The gold plum is a herbaceous annual plant belonging to the genus Hibiscus. It is also called a wild bamboo plant. It is a medicinal plant planted in the mainland gangbok. It has been restored and grown in China. In recent years, It is growing in Korea.

In July to August, the gold coins grow in excess of the height of a man. The diameter of the blossoms is usually 16 to 18 cm. The petals are yellow, thin and scarlet, and the leaves are very beautiful, with dark blue leaves. It is said that it is very important to harvest on time because it is only about six hours to bloom.

The flower of the gold coin is cut from the end of July and is torn and dried. Cosmetics and tea are used as raw materials, and gold leaf is also used for various purposes because it contains various physiologically active substances.

The gold leaf powder or gold leaf extract according to the present invention may be powdery or an extract obtained by arbitrarily mixing 2, 3 or all of flowers, leaves, branches and roots of gold leaf, As a powder or an extract thereof.

The preparation of gold powder can be used without any particular limitation as long as it is widely known in the field to which the present invention belongs. Gold extract can be produced using extraction and separation methods widely known in the field to which the present invention belongs.

As the solvent suitable for extracting the extract from gold leaf, any solvent acceptable in the field of the present invention may be used, and water or an organic solvent may be used. Examples of the solvent include alcohols having 1 to 4 carbon atoms, acetone, ether, and the like, including purified water, methanol, ethanol, propanol, isopropanol, butanol, Various solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. But is not limited to.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used.

In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation to the method for producing the gold coin extract of the present invention, and any known method can be used.

For example, the gold extract of the present invention may be prepared in powder form by an additional process such as vacuum distillation, freeze drying, or spray drying.

Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, the term "extract" is a concept including all extracts, fractions and tablets obtained in each step of extraction, fractionation or purification, their diluted solutions, concentrates or dried products.

The gold leaf extract of the present invention is preferably extracted from gold leaf with an appropriate solvent and may include, for example, a hot water extract, a polar solvent extract or a non-polar solvent extract of gold leaf.

In one embodiment of the present invention, the lyophilized gold powder is pulverized and then pulverized in an extraction solvent in an amount of about 0.5 to 20 times, preferably about 1 to 15 times the weight of the dried sample, at room temperature for about 0.5 to 48 hours Lt; / RTI > for 1 to 30 hours. Preferably, an alcoholic extract is obtained, which is then filtered and concentrated to obtain an iron acetate extract, and a fraction obtained by dissolving the crude extract in an extraction solvent and another solvent is obtained.

The papo tree used in the present invention is a deciduous broad-leaved tree native to North America. The flowering time is around April, and the fruit is harvested around September-October. The fruit has a refreshing taste like banana, pineapple and mango combined, It has a high protein content and has recently been shown to have a strong anti-cancer component, and has attracted attention as a medical and health food.

Specifically, in the present invention, papaya pulp powder, papaver leaf powder, papaya pulp extract. Papaya leaf extract, and mixtures thereof is used as an active ingredient of the composition, and the preparation of the powder and the preparation of the extract are the same as those described above.

The above mixture means a mixture of two or three random mixtures selected from papaya pulp powder, papaya leaf powder, papaya pulp extract and papaya leaf extract, all mixed together.

In the present invention,

0.01 to 70% by weight of any one selected from the group consisting of gold powder, gold powder,

A papaya fruit powder, a papaya leaf powder, a papaya fruit extract, a papaya leaf extract, and a mixture thereof may be contained in an amount of 0.01 to 70% by weight.

However, the composition is not limited thereto, and may vary depending on the condition and progress of the patient.

≪ Pharmaceutical composition >

The composition of the present invention may be a pharmaceutical composition containing a pharmaceutically acceptable carrier besides the active ingredient.

The pharmaceutical composition of the present invention can be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, A lubricant or a flavoring agent can be used.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

The form of the pharmaceutical composition is not particularly limited and may be suitably formulated according to each disease or ingredient by the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA have.

In one embodiment of the present invention, the pharmaceutical composition of the present invention may be a composition for external application for skin. The dermatological pharmaceutical composition of the present invention is an external preparation for skin having an effect of protecting skin from ultraviolet rays, and it can be used as an external preparation for skin such as cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasma But it is not limited thereto.

The preferred dosage of the extract of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the administration route and the period of time, but can be appropriately selected by those skilled in the art.

< Cosmetics  And cleaning composition>

In addition, the composition of the present invention can be used variously in cosmetics or detergents (soap, shampoo, etc.) having hair loss prevention and hair growth promoting effect.

Examples of products to which the composition of the present invention can be added include hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, soap, hair conditioner, hair treatment, Hair cream, hair massager cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave agent, hair bleaching agent, hair gel , Hair glazes, hair dressers, hair lacquers, hair moisturizers, hair mousse, hair sprays, and the like.

The composition of the present invention may contain not only the above-mentioned effective ingredient but also ingredients conventionally used in cosmetic and detergent compositions such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts Selected composition.

The water-soluble vitamin is not particularly limited as long as it can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, And their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbic acid-2-phosphate, magnesium ascorbate-2-phosphate etc.) can also be added to water-soluble vitamins .

The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable vitamins include vitamins such as vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl laurate, and the derivatives thereof (palmitic acid ascorbin, stearic acid ascorbin, dipalmitic acid ascorbin, dl-alpha tocopherol acetate, Ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be any compound as long as it can be compounded in cosmetics, and examples thereof include collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, and keratin. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be any compound as long as it can be incorporated in cosmetics, and examples thereof include hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be any as long as they can be incorporated into cosmetics, and preferable examples thereof include ceramides, phytosphingosine and sphingoglycolipids. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be any of those which can be compounded in cosmetics. Preferably, the seaweed extract is selected from the group consisting of algae extract, red pepper extract, green algae extract and the like. Also, the algae extract may be colored guanine, arginic acid, Potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic or detergent of the present invention may be blended with other necessary components in addition to the above essential ingredients. Examples of the compounding ingredients include organic solvents such as a preservative, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, Accelerators, coolants, antiperspirants, purified water, and the like.

Examples of the oil retaining component include ester-based oil retaining, hydrocarbon-based oil retaining, silicone-based oil retaining, fluoric oil retaining, animal retention and plant retention.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearyl hydroxystearic acid isostearyl, 12-stearoyl hydroxystearic acid stearyl, 12-stearoyl hydroxystearic acid stearyl, One hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, hardened castor oil, and the like.

Examples of the moisturizing agent include water-soluble low-molecular moisturizing agents, oil-soluble molecular moisturizing agents, water-soluble polymers, and oil-soluble polymers.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. .

Examples of the oil-soluble polymer include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid and lanolin fatty acid cholesteryl ester.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE POE / POP alkyl ethers, polyether modified silicones, polyether modified polyesters, polyoxyethylene / polyoxypropylene copolymers, polyoxyethylene / polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include inorganic pigments such as silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, Bengala, clay, bentonite, titanium mica, titanium oxide, bismuth chloride, zirconium oxide, magnesium oxide, Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium hydroxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Silk powder, cellulose, CI Pigment Yellow, CI Pigment Orange, and composite pigments of inorganic pigments and organic pigments thereof.

As the organic powder, metallic soap such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethylmethacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride, and the like.

Examples of ultraviolet absorbers include paraaminobenzoic acid, ethyl parnamobenzoate, amyl paranobenzoate, octyl paranobenzoate, ethyleneglycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamate , Octyl methoxycinnamate, dioctyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl paratumoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Carninoic acid, ethyl urocanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino-p- (carbo-2'-ethylhexyl- , 3,5-triazine, 2- (2-hydroxy Methyl-5-methylphenyl) benzotriazole, and the like.

Examples of the disinfectant include hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, zinc filitione, benzalkonium chloride, No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and eicosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, any of the above components may be blended within the range that does not impair the objects and effects of the present invention, but is preferably 0.01 to 5% by weight, More preferably 0.01 to 3% by weight.

Such formulations may take the form of solutions, emulsions, viscous mixtures, and the like.

The present invention can include ingredients commonly used as active ingredients, including, for example, conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and flavoring agents.

The composition of the present invention can be prepared in any formulations conventionally produced in the technical field of the present invention, and examples thereof include emulsions, creams, lotions, packs, foundations, lotions, essences and hair cosmetics.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters.

<Food composition>

The composition of the present invention may also be a food composition. In addition to containing an active ingredient, such a food composition may contain various flavors or natural carbohydrates as an additional ingredient such as a conventional food composition.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. The above-described flavors can be advantageously used as natural flavorings (tau martin), stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.).

The food composition of the present invention can be formulated in the same manner as the above pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolates, foods, confectionery, pizza, ram noodles, other noodles, gums, candy, ice cream, alcoholic beverages, vitamin complexes, .

In addition, the above food composition may further contain flavoring agents such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and aging agents (cheese, chocolate, etc.), pectic acid, Salts of alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks and the like. In addition, the food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks.

Since the active ingredient of the present invention is a natural substance with little toxicity and side effects, it can be safely used even when taken for a long time for the purpose of preventing hair loss or promoting hair growth. The health functional food of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and circles.

In the present invention, the term "health functional food" refers to a food prepared and processed using raw materials and ingredients having useful functions in accordance with the Health Functional Food Act No. 10219, Or to obtain a beneficial effect in health use such as physiological action.

The health functional foods of the present invention may contain conventional food additives and, unless otherwise specified, whether or not they are suitable as food additives are classified according to the General Rules for Food Additives approved by the Food and Drug Administration, Standards and standards.

The items listed in the above-mentioned "Food Additive Code" include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as persimmon extract, licorice extract, crystalline cellulose, high color pigment and guar gum; L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar coloring preparations and the like.

For example, the health functional food in the form of tablets may be prepared by granulating a mixture of gold leaf extract, which is an active ingredient of the present invention, with an excipient, a binder, a disintegrant and other additives in a usual manner, Or the mixture can be directly compression molded. In addition, the health functional food of the tablet form may contain a mating agent or the like if necessary.

The hard capsule of the capsule-type health functional food can be prepared by filling a normal hard capsule with a mixture of a gold capsule extracted as an active ingredient of the present invention with an additive such as an excipient. The soft capsule may be prepared by dissolving And filling the mixture with a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

The ring-shaped health functional food can be prepared by molding a mixture of a gold leaf extract, an active ingredient of the present invention, an excipient, a binder, a disintegrant, and the like by a conventionally known method and, if necessary, Or it may be coated with a material such as starch, talc.

The granular health functional food can be prepared by granulating a mixture of gold leaf extract, which is an effective ingredient of the present invention, with an excipient, a binder, a disintegrant, etc., by a conventionally known method and, if necessary, And the like.

The health functional food may be a beverage, a meat, a chocolate, a food, a confectionery, a pizza, a ramen, a noodle, a gum, a candy, an ice cream, an alcoholic beverage, a vitamin complex and a health supplement food.

Hereinafter, the present invention will be further described by way of examples. The examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

<Statistical Analysis>

All measurements were expressed as means ± standard error. Statistical significance was tested with student's t test. Significance was noted when the p-value was less than 0.05. Statistical analysis was performed using SPSS 12.0K for Windows (Release 12.0.1, SPSS Inc. USA).

[ Example  1] The composition according to the present invention and Comparative Example  Composition manufacturing

- Gold coin  Preparation of Root Ethanol Extract -

The collected gold leaf roots were washed, dried for 48 hours, dried and finely ground. 10 g of pulverized gold leaf roots were added to 200 mL of 80% ethanol and extracted at room temperature for 24 hours. The extract was filtered with a membrane filter (0.45 μm, Whatman, Maidstone, England). The extracts of 80% ethanol extract of Kyoho Kyoji were removed with organic solvent using a vacuum concentrator and lyophilized. The final concentration of dimethyl sulfoxide (DMSO) was not exceeded by 0.2% in the experiment by dissolving the 80% ethanol extract of the gold leaf Kyusyu in the form of lyophilized powder with dimethyl sulfoxide (DMSO).

- Gold coin  Production of leaf ethanol extract -

Was prepared in the same manner as in the above-described gold coin roots.

- Gold coin  Manufacture of Flower Ethanol Extract -

Was prepared in the same manner as in the above-described gold coin roots.

- Gold coin  Preparation of branched ethanol extracts -

Was prepared in the same manner as in the above-described gold coin roots.

- Papo tree  Production of ethanol extract of fruit pulp -

Was prepared in the same manner as in the above-described gold coin roots.

- Papo tree  Production of leaf ethanol extract -

Was prepared in the same manner as in the above-described gold coin roots.

- Heat number Chestnut  Produce-

Except that the ethanol extract was added to 200 mL of purified water and the mixture was heated to 100 ° C. and extracted for 24 hours.

- Example  And Comparative example  Produce-

As shown in Tables 1, 2 and 3, the compositions of Examples and Comparative Examples were prepared and used in the tests described below, and the mixing ratios were set to the same weight ratios.

Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Gold coin  Root
Ethanol extract ○ ○ ○ Gold coin  leaf
Ethanol extract ○ ○ Gold coin  Branch
Ethanol extract ○ ○ Gold coin  Flower
Ethanol extract ○ ○ Papo tree  pulp
Ethanol extract ○ ○ ○ Papo tree  leaf
Ethanol extract ○ ○ ○ ○

Example 1 Example 8 Example 9 Example 10 Example 11 Example 12 Gold coin  Root
Heat number  extract ○ ○ Gold coin  leaf
Heat number  extract ○ ○ Gold coin  Branch
Heat number  extract ○ ○ Gold coin  Flower
Heat number  extract ○ ○ ○ Papo tree  pulp
Heat number  extract ○ ○ ○ Papo tree  leaf
Hot water extract ○ ○ ○ ○

Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Gold coin  leaf
Heat number  extract ○ Gold coin  Flower
Heat number  extract ○ Papo tree  pulp
Heat number  extract ○ Papo tree  leaf
Heat number  extract ○

[ Experimental Example  One] Of dermal papilla cells  Proliferation efficacy

Examples and comparative examples were examined using immortalized dermal papilla cells (Rat vibrissa immortalized dermal papilla cells) for the growth promoting effect of dermal papilla cells. Dermal papilla cells are the mesoderm-derived cells located at the base of the hair follicle, and they are known to play an important role in hair formation and growth through interaction with epithelial cell matrix cells.

Immortalized dermal papilla cells isolated from rat beard (Rat vibrissa immortalized dermal papilla cells) were treated with 100 units / ml penicillin-100 ㎍ / ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated fetal bovine serum (FBS; The cells were cultured in DMEM (Hyclone Inc, USA) medium containing Gibco Inc, NY, USA at 37 ° C in a 5% CO2 incubator and subcultured once every 3 days. The proliferation of dermal papilla cells was measured using MTT assay. 1.0 × 10 ⁴ cells / ml of dermal papilla cells were cultured in DMEM medium containing 1% FBS, and cultured for 24 hours in a 96-well plate. Then, 100 μg / ml of each of the examples and the comparative examples were treated. Minoxidil (Sigma, MO, USA), a positive control, was treated at a concentration of 10 μM. After 4 days of incubation, 50 μl of MTT (Sigma, MO, USA) was added and reacted for 4 hours. The supernatant was removed and 200 μl of DMSO was added to dissolve the precipitate and the absorbance was measured at 540 nm using a microplate reader (Amersham Pharmacia Biotech, NY, USA). The average absorbance of each sample group was determined. The absorbance of the control group was taken as 100, and the degree of propagation was examined. The results are shown in Table 4 below.

Cell proliferation value The positive control (minoxidil) 109.3 Example 1 132.5 Example 2 133.4 Example 3 133.7 Example 4 141.5 Example 5 142.7 Example 6 141.2 Example 7 133.1 Example 8 135.8 Example 9 132.7 Example 10 138.2 Example 11 139.3 Example 12 141.2 Comparative Example 1 111.3 Comparative Example 2 112.7 Comparative Example 3 104.4 Comparative Example 4 104.7

In Table 4, it can be seen that Examples 1 to 12, which are the compositions according to the present invention, showed more proliferation of dermal papilla cells than the positive control group and Comparative Examples 1 to 4.

[ Experimental Example  2] 5α-Reductase Inhibitory Effectiveness Evaluation

Dihydrotestosterone (DHT) leads to progressive miniaturization of dermal papilla cells and hair follicles and inhibits hair growth. The conversion of testosterone to dihydrotestosterone (DHT) has been shown to occur by 5α-reductase activity have. These results suggest that a decrease in dihydrotestosterone (DHT) through inhibition of 5α-reductase activity is necessary for hair growth. Therefore, we investigated whether 5-α-reductase inhibitory effect, which plays a very important role in hair growth, is obtained by using coenzyme of SD rat prostate 7 ~ 8 weeks of age.

Male SD rats aged 7 to 8 weeks were anesthetized with ethyl ether, and cervical vertebrae were slaughtered to remove the prostate gland and stored in liquid nitrogen. The prostate gland was placed in a homogenizer buffer (20 mM potassium phosphate buffer, pH 6.6, 0.32 M sucrose, 1 mM dithiothreitol (DTT), 0.2 mM PMSF) and pulverized using a Polytron homogenizer (Brinkman instruments, Wesrbury, NY, USA). After centrifugation (100,000 g, 90 min, 4 ° C), the obtained pellet was washed twice and suspended in a homogenizer buffer and used as a crude enzyme.

The 5α-reductase assay was performed as follows. The crude reaction mixture (40 mM potassium phosphate (pH 6.6), 200 μM NADPH, 1 mM DTT, and 120 nCi [1,2,6,7-3H] testosterone) was added to the microtubes and 500 μl , Followed by reaction at 37 占 폚 for 60 minutes. 1 ml of ethyl acetate was added, vortexed, and centrifuged (1,000 g, 5 minutes) to obtain supernatant. After transferring to a new microtube, it was completely dried using a heating plate (60 ° C). 50 μl of ethyl acetate containing 500 μg / ml testosterone and 500 μg / ml dihydrotestosterone (DHT) was added to the microtube and spotted on a TLC plate. The TLC plate was developed in a developing solvent (50% cyclohexane, 50% ethyl acetate) and then dried. Testosterone was identified at 254 nm with a UV spectrometer and 10% sulfuric acid was sprayed to identify dihydrotestosterone (DHT). Radioactivity was measured using a liquid scintillation analyzer (Packard Bioscience, Meriden, USA) with 10 ml of Ultima Gold ^™ cocktail (PerkinElmer, Massachusetts, USA) after clipping testosterone and dihydrotestosterone (DHT) The conversion of testosterone by 5α-reductase activity was calculated as the ratio of dihydrotestosterone (DHT) / (testosterone + dihydrotestosterone (DHT)) × 100.

The results are shown in Table 4, and the results are shown in Table 4. The results are shown in Table 4.

Cell proliferation value The positive control (pinasteride) 92.6 Example 1 91.1 Example 2 89.7 Example 3 91.7 Example 4 92.7 Example 5 94.7 Example 6 95.5 Example 7 90.2 Example 8 91.4 Example 9 88.6 Example 10 92.4 Example 11 93.3 Example 12 94.2 Comparative Example 1 36.7 Comparative Example 2 38.4 Comparative Example 3 25.2 Comparative Example 4 27.7

As shown in Table 5, the compositions of Examples 1 to 12 according to the present invention showed a suppressive effect at a level almost similar to that of the positive control, and showed a higher inhibitory effect than those of Comparative Examples 1 to 4.

(P> 0.05), 28.9 ± 4.6%, 36.7 ± 1.8% (P> 0.01) and 19.5 ± 4.5%, respectively, in the inhibitory effect of 5α-reductase. This inhibitory effect of 5α-reductase is slightly lower than the inhibitory effect of finasteride (90.6%) used as a positive control substance (see FIG. 2), but the gold leaf extract has a 5α-reductase enzyme like finasteride Lt; RTI ID = 0.0 &gt; inhibitor. &Lt; / RTI &gt;

[ Experimental Example  3] in in vitro  Hair growth efficacy measurement

3-1. Rat Isolation and culture of rat rat hair follicles (vibrissa follicles)

Male Wistar rats, 3 weeks old, were purchased from Central Japan laboratory animals (Japan SLC, Hamamatsu, Janpan), anesthetized with ethyl ether and slaughtered. The left and right mysticial pads of the rat were separated and the E / P buffer (EBSS, Sigma MO, USA) containing 100 units / ml penicillin-100 μg / ml streptomycin (Gibco Inc, USA) + phosphate-buffered saline (PBS, Sigma MO, USA). Observation was made under a dissecting microscope and the vibrissa follicles were carefully removed. The hair follicles were placed in a Petri dish containing E / P buffer and incubated for 1 hour at 37 ° C in a 5% CO2 incubator. (Gibco Inc, NY, USA), 10 μg / ml insulin (Sigma MO, USA), 50 nM hydrocortisone (Sigma MO, USA) and 100 units / ml penicillin-100 500 μl of William E medium (Gibco Inc, NY, USA) containing 1 μg / ml streptomycin was added to each well and incubated at 37 ° C. in a 5% CO 2 incubator. One experimental group used 8-12 hair follicles, and the medium was changed every 3 days during culture. The comparative examples and the examples were treated at a concentration of 100 μg / ml, and the positive control substance minoxidil was treated at a concentration of 10 μM.

3-2. Rat (rat) Measured growth of the hair follicles (vibrissa follicles)

Growth of vibrissa follicles of 3-week-old rat was separated and cultured for 3 weeks, and hair follicle length was measured to determine hair growth promoting efficacy. The shape of the cultured cortical follicle (vibrissa follicle) was photographed using a microscope (Olympus, Japan). Follicular length was measured using an image analyzer (DP controller; Olympus, Japan). The average value of the hair follicle length change was obtained and compared with the average length of the control group, the degree of growth was measured, and the results on the 22nd day of the experiment are shown in Table 6.

Cell proliferation value The positive control (minoxidil) 116.8 Example 1 125.8 Example 2 127.2 Example 3 125.2 Example 4 131.4 Example 5 128.8 Example 6 129.9 Example 7 124.7 Example 8 126.2 Example 9 130.1 Example 10 127.3 Example 11 128.5 Example 12 130.4 Comparative Example 1 104.2 Comparative Example 2 109.5 Comparative Example 3 87.7 Comparative Example 4 81.2

In Table 6, it can be seen that Examples 1 to 12, which are the compositions according to the present invention, showed more proliferation of dermal papilla cells than the positive control group and Comparative Examples 1 to 4.

As described above, it is believed that a mixture of a goldfish and a follicle proliferates dermal papilla cells, inhibits the activity of 5α-reductase and inhibits the activity of vibrissa follicles, and thus the present invention can be used as a composition useful for preventing hair loss and improving hair growth.

Various formulations of the composition containing the extract of the present invention will be described below, but the present invention is not intended to be limited thereto but is specifically described.

[ Formulation example  1] Manufacture of hair tonic

The composition of the present invention 10.0%

Resorcinol 0.1%

Menthol 0.05%

Panthenol 0.2%

Salicylic acid 0.1%

Tocopheryl acetate 0.1%

0.1% of pyridoxine hydrochloride

Castor oil 5.0%

Pigment amount

Fragrance quantity

Ethanol quantity

Purified water balance

Total 100.0%

[ Formulation example  2] Hair Conditioner  Produce

The composition of the present invention 2.5%

Cetanol 3.5%

Self-emulsifiable glycerin monostearate 1.5%

Propylene glycol 2.5%

Stearylmethylbenzylammonium chloride (25%) 7.0%

Methyl paraoxybenzoate 0.3%

Pigment amount

Fragrance quantity

Purified water balance

Total 100.0%

[ Formulation example  3] Hair lotion  Produce

The composition of the present invention 5.0%

Resorcinol 2.0%

Menthol 2.0%

Panthenol 0.5%

0.0 &gt; 0.1% &lt; / RTI &

Fragrance, coloring 0.5%

Purified water balance

Total 100.0%

[ Formulation example  4] Hair soap  Produce

The composition of the present invention 0.1%

Titanium dioxide 0.2%

Polyethylene glycol 0.8%

Glycerin 0.5%

Ethylenediamine tetraacetic acid 0.05%

Sodium 1.0%

Pigment amount

Soap perfume

Make-up soap base (moisture content 13%, parts by weight) Remaining amount

Total 100.0%

[ Formulation example  5] Hydrophilic ointment

0.5 mg of the composition of the present invention

250 mg of white vaseline

Stearyl alcohol 220 mg

0.25 mg of ethyl (or methyl) p-oxybenzoate

120 mg of propylene glycol

15 mg of sodium lauryl sulfate

Propyl p-oxybenzoate 0.15 mg

[ Formulation example  6] Manufacturing of beverages

10 mg of the composition of the present invention

Calcium lactate 50 mg

Citric acid 5 mg

Nicotinic acid amide 10 mg

3 mg of sodium riboflavin hydrochloride

Pyridoxine hydrochloride 2 mg

Arginine 10 mg

Sucrose fatty acid ester 10 mg

200 ml of purified water

The present invention has been described with reference to the preferred embodiments, experimental examples and preparation examples. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments, examples, and examples should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (4)

Gold powder, gold powder, gold powder, and a mixture thereof; And
A composition for preventing hair loss or improving hair growth comprising any one selected from the group consisting of papaya pulp powder, papaya leaf powder, papaya pulp extract, papaya leaf extract, and mixtures thereof.
The method according to claim 1,
Wherein the gold powder is any one selected from the group consisting of gold leaf powder, gold leaf powder, gold leaf powder, gold leaf powder, and mixtures thereof,
Wherein the gold extract is selected from the group consisting of gold leaf extract, gold leaf extract, gold leaf extract, gold leaf extract, and mixtures thereof.
3. The method according to claim 1 or 2,
Wherein the extract is extracted with an extraction solvent selected from the group consisting of water, C1-C4 alcohol, hexane, ethyl acetate, butylene glycol, propylene glycol, glycerin, acetic acid, ether, chloroform, Or hair growth inhibiting composition.
3. The method according to claim 1 or 2,
0.01 to 70% by weight of any one selected from the group consisting of the above-mentioned gold powder, the gold powder and the mixture thereof,
Wherein said composition comprises 0.01 to 70% by weight of any one selected from the group consisting of papaya pulp powder, papaya leaf powder, papaya pulp extract, papaya leaf extract and mixtures thereof. Composition.