KR101498200B1 - A Composition Comprising the Extract of Codium Platylobium for Preventing Hair Loss or Improving Hair Growth - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or hair growth comprising an extract of Codium platylobium as an active ingredient, wherein the composition for preventing hair loss or hair growth, which comprises the extract according to the present invention as an active ingredient, And has an effect of inhibiting reductase activity. Therefore, the composition of the present invention is useful as a raw material for functional cosmetics, medicines and foods for hair loss prevention and hair growth improvement, and is advantageous in that it is safe for long-term use as a natural substance.

Description

[0001] The present invention relates to a composition for preventing hair loss or improving hair growth, which comprises an extract of Alaska pollack as an active ingredient,

The present invention relates to a composition for preventing hair loss or hair growth, which comprises a band-and-hearing extract as an active ingredient. More specifically, the present invention relates to a composition for hair loss prevention or hair growth improvement having an effect of inhibiting 5α-reductase.

Hair loss occurs in many men and women and causes emotional distress. Hair loss has been found to have features such as an increase in vellus hair, a reduction in follicles, and a decrease in growing follicles. Androgenetic alopecia (AGA) is the most common form of hair loss occurring in men over 40 years of age. Although the study on the development of therapeutic agents and the mechanism of the increase of the hair loss population is actively carried out, it is not known exactly what causes the hair loss.

Drugs that promote hair growth are now approved by the US Food and Drug Administration (FDA) and are well known for finasteride and minoxidil.

Among them, finasteride, a Type 2 5α-reductase inhibitor, has been shown to reduce hair loss by reducing dihydrotestosterone (DHT) in patients with hair loss (Van Neste et al., 2000) Enzyme is an important target for hair loss treatment.

Minoxidil is one of the hair growth promoting drugs approved by the Food and Drug Administration (FDA). It was first developed as a vasodilator for the treatment of hypertension. However, as a side effect, Is used as a hair growth promoter. Although the specific mechanism of action of minoxidil on hair growth has not yet been clearly elucidated, it is believed that K _ATP -channel opening, increased nutritional supply through vasodilation, and β-catenin pathway in dermal papilla cells Is thought to induce hair growth. Dermal papilla cells are mesenchymal-derived fibroblasts that secrete protein such as insulin-like growth factor-1 (IGF-1), hepatocyte growth factor (HGF) and fibroblast growth factor-7 (FGF-7) Minoxidil has the effect of proliferating the growth of dermal papilla cells through a decrease in the apoptosis of dermal papilla cells It is known.

Therefore, measuring the inhibitory effect of 5α-reductase activity of any substance, NIH3T3 fibroblast proliferation effect and growth proliferation effect of dermal papilla cell can be evaluated by how effectively the substance can treat hair loss and improve hair growth It can be a valid indicator.

Studies have been made to obtain a composition for preventing hair loss or hair growth from natural materials by paying attention to such a principle. Japanese Patent Laid-Open Publication No. 2003-081861 discloses a composition for improving the hair growth of Codium tenue , Codium fragile , And Codium coarctatum extract exhibited hair growth effect. However, it does not reveal the hair growth effect of Codium platylobium extract, nor does it describe the specific efficacy mechanism.

Japanese Patent Application Laid-Open No. 2003-081861

The inventors of the present invention have continuously studied on the effect of preventing hair loss and hair growth, and found that the band-and-hearing extract has an inhibitory effect on 5α-reductase activity, and that this effect is superior to other auditory and plant extract effects Thereby completing the invention.

Accordingly, it is an object of the present invention to provide a composition comprising an anti-hair-loss or hair-growth enhancing effect as an effective ingredient.

In order to accomplish the above object, the present invention provides a composition for preventing hair loss or improving hair growth, which comprises a band-and-ear extract as an active ingredient.

According to one embodiment of the present invention, the extract is prepared by mixing water, a C ₁ -C ₄ alcohol, hexane, ethyl acetate, butylene glycol, propylene glycol, glycerin, acetic acid ethyl ether, chloroform, , Preferably an alcohol extract, more preferably an ethanol extract.

In one preferred example, the banded auditory extract is an extract obtained by freeze-drying the banded hearing and then pulverizing the pulverized powder into a C ₁ to C ₄ lower alcohol solvent for extraction at room temperature for 0.5 to 48 hours.

In another preferred embodiment, the banded auditory extract is included at a concentration of 0.01 to 10 [mu] g / ml on the composition.

The composition may be used as a medicine, a health supplement, a cosmetic, a food or a food additive. That is, it can be used as a pharmaceutical composition, a cosmetic composition, a food composition, or a food additive containing an extract of a band-and-hearing extract as an active ingredient.

When the composition of the present invention is used as a pharmaceutical composition, it is formulated into a form of a skin external preparation selected from the group consisting of cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta, .

When used as a cosmetic composition, it can also be used as a hair tonic, a hair conditioner, a hair essence, a hair lotion, a hair nutrition lotion, a hair shampoo, a hair rinse, a hair treatment, a hair cream, , Hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave agent, hair bleaching agent, hair gel, hair glaze, hair dresser, hair lacquer, hair A moisturizer, a hair mousse or a hair spray.

The composition for preventing hair loss or hair growth comprising the extract according to the present invention has an inhibitory effect on 5? -Reductase activity. Therefore, it can be usefully used as a raw material for functional cosmetics, medicines and foods for hair loss prevention and hair growth improvement since it exhibits a useful effect for preventing hair loss and improving hair growth. In particular, the band-aids extract of the present invention is a natural substance, and the composition of the present invention containing it as an effective ingredient has an advantage that it is safe for long-term use.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the inhibitory effect of the Codium platylobium extract of the present invention on 5α-reductase activity. FIG. The inhibitory effect of Codium platylobium extract on 5α-reductase activity is superior to the inhibitory effect of 5α-reductase activity of Codium fragile and Codium intricatum , and especially at low concentrations of 0.1 μg / ml, (Codium fragile) and shows a hearing bush suppressed more remarkably excellent 5α- reductase activity when the (Codium intricatum) efficacy (data were expressed as mean ^{± SD, * P <0.05,} ** P <0.01, *** P < 0.001 vs. control).

Hereinafter, the present invention will be described in detail.

The present inventors have examined the hair growth promoting effect of the band-and-hearing extract using an in vitro experimental method and confirmed the inhibitory effect of 5α-reductase activity, which is known to play an important role in hair growth. Accordingly, the present invention provides a composition for preventing hair loss or improving hair growth, which comprises a band-and-throat extract as an active ingredient.

According to one embodiment of the present invention, band hearing can be collected from the southern coast such as Geoje Island and Jeju Island.

In addition, the extract of the band can be obtained by using the extraction method known in the art of the present invention. For example, after lyophilization of the freeze-dried bandage hearing is performed, about 0.5 to 20 times, preferably about The extraction is carried out at a room temperature for about 0.5 to 48 hours, preferably for 1 to 30 hours in an extracting solvent of 1 to 15 times the volume to obtain a banded auditory extract.

As the extraction solvent for extracting the band-and-ear extract, any solvent which is generally allowed may be used. According to one embodiment of the present invention, water, C ₁ -C ₄ alcohol, hexane, ethyl acetate, butylene glycol, propylene glycol, glycerin, An extraction solvent selected from the group consisting of ethyl acetate, ether, chloroform and a mixed solvent thereof may be used. Preferably, the alcohol is ethanol or methanol, more preferably 80% Ethanol may be used.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the method for producing the band-aids extract of the present invention, and any known method can be used.

For example, the banded auditory extract contained in the composition of the present invention may be prepared into a powdery state by an additional process such as vacuum distillation, freeze-drying, or spray drying. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, the term "extract" is a concept including all extracts, fractions and tablets obtained in each step of extraction, fractionation or purification, their diluted solutions, concentrates or dried products.

The band-aids extract of the present invention is preferably extracted from a band of hearing using a suitable solvent, and may include, for example, a hot-water extract of a banded hearing, a polar solvent-soluble extract, or a non-polar solvent-soluble extract.

In one embodiment of the present invention, the band-and-hearing extract may be included at a concentration of 0.01-10 μg / ml, preferably at a concentration of 0.1 μg / ml, relative to the composition. However, the composition is not limited thereto, and may vary depending on the condition and progress of the patient.

Pharmaceutical formulation

The composition of the present invention may be formulated into a pharmaceutical formulation containing a band-aids extract as an active ingredient and a pharmaceutically acceptable carrier.

The pharmaceutical composition of the present invention may be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, A lubricant, a lubricant or a flavoring agent may be used.

The composition formulated into the pharmaceutical formulation may contain, in addition to the above-mentioned effective ingredients, at least one pharmaceutically acceptable carrier for administration.

The preparation method is not particularly limited, and a method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA, etc. can be selected depending on each disease or ingredient.

According to one embodiment of the present invention, the composition formulated into the pharmaceutical formulation may be a composition for external application for skin, and may be a cream, gel, patch, spray, ointment, , Liniment, pasta or cataplasma, but is not limited thereto.

The preferred dosage of the composition formulated into the pharmaceutical formulation of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the administration route and the period of time, but can be appropriately selected by a person skilled in the art. However, in order to obtain the desired effect, it is preferable to administer it at 0.0001 to 100 mg / kg per day, preferably 0.001 to 10 mg / kg per day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

Cosmetic formulation

According to one embodiment of the present invention, the composition of the present invention can be variously applied to hair cosmetics which are formulated into a cosmetic formulation and have hair growth promoting effect.

According to one embodiment of the present invention, the content of the band-and-throat extract is 0.01 to 70% by weight, preferably 1.0 to 50% by weight based on the total weight of the composition formulated into the cosmetic formulation. If the content of the extract is less than 0.01% by weight, the effect of preventing hair loss or improving hair growth is greatly reduced. If the content of the extract is more than 70% by weight, skin irritation may be caused.

According to an embodiment of the present invention, the composition may be used as a hair tonic, a hair conditioner, a hair essence, a hair lotion, a hair nutrition lotion, a hair shampoo, a hair conditioner, a hair treatment, a hair cream, Hair cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave agent, hair bleaching agent, hair gel, hair glaze, hair dresser , A hair lacquer, a hair moisturizer, a hair mousse or a hair spray, but the present invention is not limited thereto.

When the composition of the present invention is formulated into a cosmetic formulation, the composition of the present invention may contain all the ingredients commonly used in cosmetics in addition to the above-mentioned extracts. Examples of the composition include water-soluble vitamins, oil-soluble vitamins, polymer peptides, Lipids, and seaweed extracts.

As the water-soluble vitamin, water-soluble vitamins can be selected as long as they can be compounded in cosmetics. Preferably, vitamin B, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H (Sodium thiamine hydrochloride, sodium ascorbate, etc.) or derivatives thereof (sodium ascorbic acid-2-phosphate, magnesium ascorbic acid-2-phosphate etc.) can also be used in the present invention Contained in water-soluble vitamins. The water-soluble vitamin can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzymatic method, or a chemical synthesis method.

Usable Vitamins Any of vitamins A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol) Alpha-tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, d-pantothenic alcohol, and the like, and derivatives thereof (such as palmitic acid ascorbin, stearic acid ascorbin, dipalmitic acid ascorbin, Pantothenyl ethyl ether, etc.) are also included in the usable vitamins used in the present invention. Usability Vitamins can be obtained by a conventional method such as a microorganism conversion method, a purification method from a culture of a microorganism, an enzyme or a chemical synthesis method.

The polymeric peptide may be selected from any of those that can be compounded in the cosmetic, and preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be selected. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be selected from any of those that can be compounded in the cosmetic, and preferably hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be selected. For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be selected from any of those that can be incorporated in cosmetics, but ceramides, phytosphingosine, sphingoglycolipids and the like may be preferably selected. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The seaweed extract may be selected from any of those which can be compounded in cosmetics. Preferably, the seaweed extract may be selected from algae extract, red pepper extract, green algae extract, and the like. From these seaweed extracts, Sodium, potassium alginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained from seaweed by a conventional method.

The cosmetic of the present invention may contain, in addition to the above-mentioned essential ingredients, other ingredients usually added to cosmetics, if necessary. In this case, the compounding ingredient may be a preservative, a moisturizer, an emollient, a surfactant, an organic or inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, , A cold agent, an antiperspirant agent, purified water, etc. may be used.

Examples of the above-mentioned fat-soluble ingredients include ester-based fats, hydrocarbon-based fats, silicon-based fats, fluorine-based fats, animal fats and plant fats.

Examples of ester-based fats include glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearyl isostearate, Butyl isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, isopropyl myristate, butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isosilyl myristate, isostearic acid isostearyl, isostearyl palmitate, octyldodecyl myristate, Trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic acid pentaerythritol tetra (2-ethylhexanoate) , Decyl caprylate, decyl laurate, hexyl laurate, myristate decyl, myristyl myristate, myristine monoethyl stearate, stearyl stearate, decyl oleate, ricinoleic acid tri , Isostearyl stearate, isostearyl stearate, isodecyl stearate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, isopropyl stearate, isopropyl stearate, isopropyl stearate, -Hexyl stearate, stearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, di (capryl, capric acid) propylene glycol, Propyleneglycol propionate, propyleneglycol propionate, dicaproic acid neopentyl glycol, dioctanoic acid neopentyl glycol, tricarboxylic acid glyceryl, triunsaturated glyceryl, triisopalmitic acid glyceryl, triisostearic acid glyceryl, neopentanoic acid octyldodecyl Octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, octanoic acid octanoate, Octyldecyl lactate, octyldecyl lactate, octyldecyl lactate, polyglycerin oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisobutyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, But are not limited to, ethyl, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, But are not limited to, dioctyl sebacate, stearic acid cholesteryl, isostearic acid cholesteryl, hydroxystearic acid cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearic acid pitostearyl, Stearoyl hydroxystearic acid isostearyl, 12-stearoyl stearyl hydroxystearate, 12-stearo Monohydroxystearic acid, and sostearyl.

Examples of the hydrocarbon hydrocarbon-based fats include hydrocarbon fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, floating isoparaffin, polybutene, microcrystalline wax and vaseline.

Examples of silicone based oils include polymethyl silicone, methylphenyl silicone, methyl cyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstarchoxysiloxane copolymer, alkyl Modified silicone oils, and amino-modified silicone oils.

Examples of the fluorine-based oil include perfluoropolyether and the like.

Examples of animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, new flower oil, soybean oil, corn oil, rape oil, apricot kernel oil, palm kernel oil, palm oil, castor oil, , Corn oil, palm oil, palm oil, cucumber nut oil, wheat germ oil, rice germ oil, shea butter, coltsfoot colostrum, marker daisy nut oil, mead home oil, egg oil, , Canned wax, carnauba wax, liquid lanolin, and hardened castor oil.

As the moisturizing agent, a water-soluble low molecular weight moisturizer, a liposoluble molecular moisturizer, a water-soluble polymer moisturizer, and a oil-soluble polymer moisturizer can be used.

Examples of the water-soluble low-molecular moisturizing agent include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B Glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactic acid salt and the like.

Examples of the lipid-soluble low-molecular moisturizing agent include cholesterol and cholesterol ester.

Examples of the water-soluble polymer moisturizing agent include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, .

Examples of the oil-soluble polymer moisturizing agent include polyvinylpyrrolidone / eicosene copolymer, polyvinylpyrrolidone / hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollients include long chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Examples of the surfactant include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants.

Examples of the nonionic surfactant include self emulsifying monostearate glycerin, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbit fatty acid ester, POE POE / POP alkyl ethers, polyether modified silicones, polyether modified polyesters, polyoxyethylene / polyoxypropylene copolymers, polyoxyethylene / polyoxypropylene (POE) Alkanolamides, alkylamine oxides, hydrogenated soybean phospholipids, and the like.

Examples of the anionic surfactant include fatty acid soap, alpha-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkylsulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl ginseng salt, Alkylsulfosuccinic acid salts, acylated hydrolyzed collagen peptide salts, and perfluoroalkyl phosphoric acid esters, and the like can be mentioned. have.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium chloride, Benzalkonium, diethylaminoethylamide stearate, dimethylaminopropylamide stearate, quaternary ammonium salts of lanolin derivatives, and the like.

Examples of the amphoteric surfactant include carboxybetaine type, amide betaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amide amine type Amphoteric surfactants and the like.

Examples of the organic and inorganic pigments include silicic acid, anhydrous silicic acid, magnesium silicate, talc, sericite, mica, kaolin, spinach, clay, bentonite, titanium coated mica, bismuth oxychloride, zirconium oxide, magnesium oxide, Inorganic pigments such as aluminum, calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, chromium oxide, chromium oxide, chromium oxide, But are not limited to, polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluororesin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, Organic pigments such as silk powder, cellulose, CI Pigment Yellow and CI Pigment Orange, and composite pigments of these inorganic pigments and organic pigments can be used.

Examples of the organic powder include metal soaps such as calcium stearate; Metal salts of alkyl phosphates such as sodium zinc cetylate, zinc laurylate and calcium lauryl laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc and N-lauroylglycine calcium; Amidosulfonic acid multivalent metal salts such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; Such as N-epsilon-lauroyl-L-lysine, N-epsilon-palmitoylidene, N-alpha-paratyylnitine, N-alpha-lauroyl arginine, Acyl basic amino acids; N-acylpolypeptides such as N-lauroylglycylglycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid, alpha-aminoaurauric acid, and the like; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like can be used.

Examples of the ultraviolet absorber include ultraviolet absorbers such as p-aminobenzoic acid, ethyl p-aminobenzoate, amyl paranobenzoate, octyl p-aminobenzoate, ethylene glycol salicylate, phenyl salicylate, benzyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, Benzyl, 2-ethoxyethyl methoxycinnamate, octyl methoxycinnamate, mono-2-ethylhexane glyceryl dipyrromethoxycinnamate, isopropyl methoxycinnamate, diisopropyl-diisopropyl cinnamate ester mixture, Dihydroxymethoxybenzophenone sulfonic acid, dihydroxybenzophenone sulfonic acid, dihydroxybenzophenone sulfonic acid and its salt, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzo Phenanone, tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianylino- p- (carbo-2'-ethylhexyl- 1,3,5-triazine, 2- ( 2-hydroxy-5-methylphenyl) benzotriazole, and the like.

Examples of the bactericide include antibiotics such as hinokitiol, trichloroacid, trichlorohydroxydiphenyl ether, crohexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, Photosensitive monomer No. 301, mononitro and eicol sodium, and undecylenic acid.

Examples of the antioxidant include butylhydroxyanisole, gallic acid propyl, and erosorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fumaric acid, sodium fumarate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate and the like.

As the alcohol, a higher alcohol such as cetyl alcohol may be used.

Any of the above components may be blended within the range not to impair the objects and effects of the present invention, but is preferably 0.01 to 5% by weight based on the total weight, More preferably 0.01 to 3% by weight.

The composition formulated into the cosmetic formulations of the present invention may take the form of solutions, emulsions, viscous mixtures, and the like.

The ingredients contained in the composition formulated in the cosmetic formulations of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetics in addition to the above extracts, for example, stabilizers, solubilizers, vitamins, And the like.

The composition of the present invention may be prepared in any form conventionally produced in the art and may be formulated into various forms such as emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic .

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

In the case of the solution or emulsion of the present invention, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolenic derivatives or ethoxylated glycerol fatty acid esters can be used.

Food formulation

The composition of the present invention may also be a composition formulated into a food preparation. Such a composition may contain various flavors or natural carbohydrates as an additional ingredient in addition to a band-aids extract as an active ingredient.

Examples of the natural carbohydrates include conventional sugars such as monosaccharides (glucose, fructose, etc.), disaccharides (maltose, sucrose etc.) and polysaccharides (dextrin, cyclodextrin and the like) or xylitol, sorbitol and erythritol The same sugar alcohol may be used. As the flavor, natural flavoring agents (tau martin), stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) or synthetic flavors (saccharine, aspartame, etc.) may be used.

The composition formulated into the food formulation of the present invention may be formulated in the same manner as the composition formulated in the pharmaceutical formulation and used as a functional food or may be added to various foods. Examples of foods to which such a composition can be added include beverage, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, gum, candy, ice cream, alcoholic beverages, vitamin complexes, have.

In addition to the active ingredients, the composition may also contain various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring agents and thickening agents (such as cheese and chocolate), pectic acid and its salts, Alginic acid and its salts, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks and the like. In addition, the composition may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks.

Since the bandage-auditory extract as an active ingredient of the present invention is a natural substance with little toxicity and side effects, it can be safely used even for long-term use for the purpose of preventing hair loss or promoting hair growth. The health functional food of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and circles.

In the present invention, the term "health functional food" refers to foods prepared and processed using raw materials and ingredients having useful functions in accordance with the Health Functional Food Act No. 10219, and the nutrients Or to obtain a beneficial effect in health use such as physiological action.

The above health functional foods may contain conventional food additives and, unless otherwise specified, conform to the general requirements of the Food Additives Ordinance approved by the Korea Food and Drug Administration, Judge by standards.

Examples of the food additives listed above include chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as persimmon extract, licorice extract, crystalline cellulose, high color pigment and guar gum; L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar coloring preparations and the like.

The health functional food in tablet form may be obtained by granulating a mixture obtained by mixing the active ingredient of the present invention with a excipient, a binder, a disintegrant and other additives, granulating it in a conventional manner, The mixture can be directly compression molded. In addition, the health functional food of the tablet form may contain a mating agent or the like if necessary.

The hard capsule of the capsule form of the health functional food can be prepared by filling a normal hard capsule with a mixture obtained by mixing the active ingredient of the extract of the present invention with an additive such as an excipient and the soft capsule may be prepared by mixing the lip- And filling the mixture with a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

The ring-shaped health functional food can be prepared by molding a mixture of a banded hearing extract, an active ingredient of the present invention, an excipient, a binder, a disintegrant, etc. by a conventionally known method and, if necessary, Or it may be coated with a material such as starch, talc.

The granular health functional food may be prepared by granulating the mixture of the active ingredient of the present invention and the excipient, excipient, binder, disintegrant, etc. into a granular form according to a known method. If necessary, a flavoring agent, And the like.

The health functional food may be a beverage, a meat, a chocolate, a food, a confectionery, a pizza, a ramen, a noodle, a gum, a candy, an ice cream, an alcoholic beverage, a vitamin complex and a health supplement food.

Statistical analysis

All measurements were expressed as mean ± SD. Statistical significance was tested by student's t test and significance was considered when p-value was less than 0.05. Statistical analysis was performed using SPSS 12.0K for Windows (Release 12.0.1, SPSS Inc. USA).

Hereinafter, the present invention will be further described by way of examples. The examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

[Examples] Band hearing ( Codium platylobium ) Preparation of extract

To the inventors to compare the hair growth effects of different species of plant extracts belonging to the hair growth effects were used to collect the band hearing (Codium platylobium) in Jeju coast, band hearing extract the hearing and hearing (Codium fragile) and bush hearing ( Codium intricatum ) was collected and used as a control.

The auditory, auditory and bush hearing points were collected and washed several times with water to remove foreign matter. The frozen samples were dried in a deep-sea freezer (-70 ° C) and powdered to a size of 50 mesh or less. 10 g of pulverized hearing, hearing and bush hearing were extracted in 200 mL of 80% ethanol for 24 hours at room temperature. The extracts were filtered with a membrane filter (0.45 μm, Whatman, Maidstone, England). The extracts of 80% ethanol extracts were stripped of organic solvent using a vacuum evaporator and lyophilized. The lyophilized 80% ethanol extract of lyophilized powder form was dissolved in dimethylsulfoxide (DMSO), and the final concentration of dimethyl sulfoxide (DMSO) was not exceeded 0.2%.

[Experimental Example] Evaluation of 5? -Reductase inhibitory effect

1. Experimental Method

Whether or not the 5-alpha-reductase inhibitory effect obtained according to the example of the present invention has an inhibitory effect on 5-alpha-reductase was investigated using coenzyme of SD rat prostate 7 to 8 weeks of age, The 5α-reductase inhibitory effect of the bark hearing extract was measured and used as a control.

Male SD rats aged 7 to 8 weeks were anesthetized with ethyl ether, and cervical vertebrae were slaughtered to remove the prostate gland and stored in liquid nitrogen. The prostate was placed in a homogenizer buffer (20 mM potassium phosphate buffer pH 6.6, 0.32 M sucrose, 1 mM dithiothreitol (DTT), 0.2 mM PMSF) and ground using a Polytron homogenizer (Brinkman instruments, Wesrbury, NY, USA). After centrifugation (100,000 g, 90 min, 4 ° C), the obtained pellet was washed twice and suspended in a homogenizer buffer and used as a crude enzyme.

The 5α-reductase assay was performed as follows. To the microtube, the crude enzyme, the banded auditory extract of the present invention and reaction buffer (40 mM potassium phosphate (pH 6.6), 200 μM NADPH, 1 mM DTT, 120 nCi [1,2,6,7-3H] testosterone) The final reaction solution was adjusted to 500 쨉 l, and reacted at 37 째 C for 60 minutes. After the reaction was completed, 1 ml of ethyl acetate was added, vortexed, and centrifuged (1,000 g, 5 minutes). The supernatant was transferred to a new microtube and completely dried using a heating plate (60 ° C).

50 μl of ethyl acetate containing 500 μg / ml testosterone and 500 μg / ml dihydrotestosterone (DHT) was added to the dried microtube and spotted on a TLC plate. The TLC plate was developed in a developing solvent (50% cyclohexane, 50% ethyl acetate) and then dried.

Testosterone and dihydrotestosterone (DHT) were identified using a UV spectrometer after the TLC plate was completely dried. Testosterone was confirmed by UV irradiation at 254 nm and dihydrootestosterone (DHT) was sprayed with 10% sulfuric acid Respectively. Radioactivity was measured with a liquid scintillation analyzer (Packard Bioscience, Meriden, USA) with 10 ml of Ultima Gold ^™ cocktail (PerkinElmer, Massachusetts, USA) after clipping testosterone and dihydrotestosterone (DHT)

The conversion of testosterone by 5α-reductase activity was calculated as [dihydrootestosterone (DHT) / (testosterone + dihydrotestosterone (DHT))] × 100. The inhibitory effect of 5α- (%) In which the testosterone conversion rate is decreased.

2. Experimental results

The band hearing (Codium platylobium) extract, 0.1, 1, and results of processing at a concentration of 10 μg / ml, respectively, 40.1 ± 7.6% (P <0.01 ), 34.6 ± 8.3% (P <0.01) and 39.8 ± 6.9% (P & Lt; 0.01 ). On the other hand, in the case of Codium fragile extract used as a control, 6.0 ± 3.7%, 15.8 ± 13.3% and 25.3 ± 11.2% ( P <0.05 ) of 5α- It inhibited the reductase activity bush hearing (Codium intricatum ) inhibited 5α-reductase activity by 0.0 ± 0.0%, 0.0 ± 0.0% and 9.5 ± 6.3% when treated with 0.1, 1 and 10 μg / ml, respectively. These auditory cords platylobium extract inhibited 5α-reductase activity by a little less than the inhibitory effect of finasteride (89.8%), but the 5-reductase inhibitor such as finasteride (See FIG. 1).

In addition, the 5α-reductase inhibitory effect of Codium platylobium extract was lower than that of 5α-reductase activity of Codium fragile and Codium intricatum , And exhibits a high 5? -Reductase activity inhibitory effect (see FIG. 1) even at a low concentration of 0.1 μg / ml, unlike plant species of other species belonging to the auditory family. Therefore, a composition useful for hair loss prevention and hair growth improvement can be prepared and used by adding a very small amount of a hair-or-herbal extract.

As described above, it was confirmed that the band-and-hearing extract has an effect of inhibiting the activity of 5? -Reductase through the above experimental examples. Therefore, the band and auditory extract of the present invention is used as a composition useful for preventing hair loss and improving hair growth .

The present invention has been described with reference to preferred embodiments and experimental examples. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments and experiments should be considered from an illustrative point of view, not from a limiting viewpoint. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

A composition for preventing hair loss or improving hair growth comprising an extract of Codium platylobium as an active ingredient. The method according to claim 1,
The extract of Codium platylobium is selected from the group consisting of water, C ₁ -C ₄ alcohol, hexane, ethyl acetate, butylene glycol, propylene glycol, glycerin, acetic acid, ether, chloroform, Wherein the composition is extracted with a selected extraction solvent. The method according to claim 1,
The extract of Codium platylobium is an extract obtained by lyophilizing Codium platylobium and then pulverizing the powder into a lower alcohol solvent of C ₁ to C ₄ for extraction at room temperature for 0.5 to 48 hours. Or hair growth. The method according to claim 1,
Wherein the extract of Codium platylobium is contained at a concentration of 0.01 to 10 μg / ml in the composition. 5. The method according to any one of claims 1 to 4,
Wherein the composition is formulated into a pharmaceutical formulation, a cosmetic formulation or a food formulation. 6. The method of claim 5,
Wherein the composition is formulated into a pharmaceutical formulation selected from the group consisting of cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta, and cataplasma. . 6. The method of claim 5,
The composition may be used in hair cosmetics such as hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair conditioner, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, Hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair moisturizers, Wherein the composition is formulated into a cosmetic formulation selected from mousse and hairspray.

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