KR101981959B1 - Cosmetic Compositions for Anti-oxidation and Moisturizing Comprising Extracts of Plants - Google Patents

Abstract

The present invention relates to a cosmetic composition for antioxidant and moisturizing activities, which includes, as an active ingredient, an extract of at least one selected from a group comprising Limonium tetragonum (Thunb.) Bullock, Suaeda malacosperma H. Hara, Boehmeria pannosa Nakai & Satake, and Fatsia japonica (Thunb.) Decne. & Planch. The extract provided by the present invention can be used as the cosmetic composition for antioxidant and moisturizing activities since the extract has excellent efficacy in DPPH scavenging activity and hyaluronic acid production.

Description

[0001] Cosmetic Compositions for Anti-oxidation and Moisturizing Comprising Extracts [

The present invention relates to an antioxidative and moisturizing cosmetic composition containing a natural plant extract as an active ingredient.

Active oxygen introduced from the outside of the living body or generated in the living body promotes aging of the living body or causes cancer. Therefore, studies on antioxidants that inhibit oxidation by active oxygen are under way, and phenolic compounds, flavonoids, tocopherol, vitamin C, selenium and the like are known. However, the natural antioxidants do not have a practically sufficient effect when applied to the skin. On the other hand, antioxidant activity is excellent and low-priced synthetic antioxidants have a problem that their use is limited due to safety concerns such as human side effects.

In addition, the skin becomes sensitive due to changes in the environment, internal stress, and external stimuli. Water retention may be reduced. As the aging proceeds, the moisture of the stratum corneum decreases and the skin becomes dry and shiny. A phenomenon such as a lost or dull appearance occurs. When the moisture of the skin decreases, the skin becomes dry, the elasticity decreases, and wrinkles are generated. In addition, the skin tone is darkened, the skin barrier function is deteriorated, and the skin becomes more sensitive to external stimuli, and various troubles can be increased. As such, the moisturizing condition of the stratum corneum is directly related to the appearance of the skin (flexibility and roughness of the skin), and the skin moisture content of the stratum corneum influences the skin membrane homeostasis associated with the condition and permeability of the skin, Since the hygroscopicity of medicines can be changed, skin moisturization is an important factor. Therefore, there is an increasing tendency for a cosmetic composition which keeps the moisture of the skin and keeps its effect so that the skin does not lose moisture and elasticity.

On the other hand, according to recent natural and environmentally friendly consumption tendency, a natural substance derived from a plant or an animal is mixed with various forms based on its functionality and used in cosmetics. In recent years, so-called " And the interest in healthy eating and health and prevention has been attracting attention.

Limonium tetragonum (Thunb.) Bullock ) is a biennial plant that grows on the seashore along the dusk of dicotyledonous plants. The roots grow thick and straight. Leaves are gathered from roots and spread in all directions, long elliptical spatula with no hairs and flat edges. The flower stalk is 30 ~ 60cm long, many branches are divided, and the flower ear of the water inflorescence runs at the end, and the length of the flower ear is 2 ~ 4cm. The bract is green, oval, and contains several flowers. The calyx is tubular and the end is divided into 5 branches. The corolla is yellow, 5 branches deeply cracked, longer than calyx. There are five stamens, one pistil, and five styles. The fruit is fusiform with a length of about 2.5mm. You can eat roots raw but smell burning hair. It is distributed in Korea, Japan (southern part) and northeastern part of China. It is effective in relieving hangover and improving liver function.

As a composition technique using Limonium tetragonum (Thunb.) Bullock extract, Korean Patent Publication No. 10-2017-0068217, " Composition for skin whitening using extract of P. gingko extract " and Korean Patent Laid- 10-2010-0040539 " Ganoderma lucidum extract having inhibitory effect on cancer cell proliferation and method for producing the same, " and yet, there is no description of a cosmetic composition capable of exhibiting antioxidative and moisturizing effects.

Suaeda malacosperma H. Hara is a perennial herbaceous herbaceous perennial plant of the dicotyledonous plant. It is a name for grass that grows in the area where sea water and fresh water meet. Leaves are alternate, linear, fleshy and pointed. The bottom is dome, the top is flat, and the vertical line is like a trail in the center. The length is 1 to 5 cm and the width is 0.2 to 0.6 cm. It is often white with a white or green color, with large leaves and small leaves. Flowers bloom in yellowish green in the leaf axil above the stem in August ~ September, forming the water inflorescence. There is no peduncle. There are five pieces of horns and egg-shaped. There are 5 stamens, a little longer than the husk, and the anther is yellow. There are two styles. The fruit is bag-shaped sac and 囊 果, which is similar to the fruit of the secondary fruit, but it is slightly smaller in size and rounded at the end. It is similar to Sehonghwamun, but it does not bloom much, and like the cushion, the upper side of the leaf is flat, but it is much wider and more sparse. The fruit is like a star like a star, but its size is slightly smaller and the end is round. It is similar to the turfgrass, but the branches are different from the lower 2 ~ 3㎝ above the stem. In other words, it looks like a combination of sea horsetail, cedar herb, bilingual herb, and turmeric. It is a species reported only in 2001 because it is difficult to distinguish it from similar species.

As a composition technique using an extract of Suaeda malacosperma H. Hara , Korean Patent Registration No. 10-1715622 entitled " Antimicrobial Composition Containing Extract of Botanicals from the Southeast of Japan, as an Active Ingredient " There is still no technology for a cosmetic composition capable of exhibiting antioxidative and moisturizing effects.

Boehmeria pannosa Nakai & Satake is a perennial plant with a perennial plant Nettle nettle and a height of about 1m. There are short hairs on the upper part of stem. Leaves are opposite, broad ovate or broad oval, thick. Both sides of the leaf are lustrous, with sharp teeth at the edge. In July ~ October, a light green flower blooms on the leaf axil, and there is a female flower on the top, and a male flower on the bottom. The fruit has an achene and hair on the upper part. It grows on the beach. It is distributed in the southern part of Korea. Wang Mossypul is an anticancer activity, antimicrobial activity, diuretic action, reduction of bleeding time and action to shorten bleeding time, uterine bleeding, diabetes, leukopenia, hematopoietic hemorrhage, cyanosis, diuretic, , Bleeding during pregnancy and abdominal pain, cystitis and other effects.

As a composition technique using the extract of Boehmeria pannosa Nakai & Satake , Korean Patent Registration No. 10-1052191 entitled " For prevention and treatment of diabetes, cancer, And Korean Patent Registration No. 10-0873372 entitled " Royal Momordicae Extract and Anticancer Agent Containing the Same " disclose only a cosmetic composition that can exhibit antioxidative and moisturizing effects .

( Fatsia japonica (Thunb.) Decne. & Planch ) is an evergreen shrub of the dicotyledonous plant Araliaceae, also called octagonal gold plate. It grows in the foothills and valleys of the beach. It is 2 ~ 3m in height. Tree bark is ashy-toned white, with several stems growing together and branching. Leaves are alternate, 20 ~ 40cm in diameter, gathered at the ends of the branches. Leaves are 7 ~ 9 pieces, split into palm and dark green, and the forked pieces are egg-shaped and have sawtooth on the edge. It is brown when it is young, but it disappears later. The petiole is 15 ~ 45cm long. The flower is 雜 性 花 (雜 性 花), bloomed in October ~ November, and runs on a large conical flower. When it is young, it is wrapped in white prisoner falling early. There are five petals, stamens, and styles. The fruit is round as a berry, and it melts black in May of the following year. It grows well in the shade, is relatively strong in pollution, and has leaf patterns. It is said that it is good for rheumatism when you put leaf in the bath in the private, It is planted for ornamental purposes and it is said to be a falcon because it is split into 8 pieces. It is distributed in Korea, Japan, and East Asia.

As a composition technique using Fatsia japonica (Thunb.) Decne. & Planch extract, in Korea, Korean Patent Registration No. 10-0663282 entitled " Method for producing extract of falciparum flesh extract for enhancing immune function of domestic falciparum muscle " And there is no technology for a cosmetic composition which can still exhibit antioxidative and moisturizing effects.

Thus, the present inventors have found that the present invention is useful for the treatment of diseases such as Limonium tetragonum (Thunb.) Bullock , Suaeda malacosperma H. Hara , Boehmeria pannosa Nakai & Satake , and Fatsia japonica (Thunb. , Exhibits excellent DPPH scavenging activity and hyaluronic acid producing activity, and thus can provide both antioxidant and moisturizing effects, and thus the present invention has been completed.

Korean Patent Publication No. 10-2017-0068217 Korean Patent Publication No. 10-2010-0040539 Korean Patent Publication No. 10-1715622 Korean Patent Publication No. 10-1052191 Korean Patent Registration No. 10-0873372 Korean Patent Registration No. 10-0663282

An object of the present invention is to provide a cosmetic composition for antioxidation and moisturization derived from natural products.

It is another object of the present invention to provide a cosmetic comprising the composition.

In order to accomplish the above object, the present invention provides a method for producing a bacterium of the genus Bacterium including Limonium tetragonum (Thunb.) Bullock , Suaeda malacosperma H. Hara , Boehmeria pannosa Nakai & Satake and Fatsia japonica ) Decne. & Planch ) as an active ingredient. The present invention also provides an antioxidative and moisturizing cosmetic composition comprising the extract as an active ingredient.

In the present invention, the extract is extracted with a solvent selected from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin, and mixed solvents thereof.

In the present invention, the extract is contained in an amount of 0.001 to 20 wt% based on the total weight of the composition.

In the present invention, the extract is characterized by having DPPH scavenging activity and hyaluronic acid producing activity.

The present invention also provides a cosmetic comprising the composition.

Since the extract provided from the present invention has excellent DPPH scavenging activity and hyaluronic acid production efficacy, it can be used as a cosmetic composition for antioxidation and moisturizing.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

Throughout this specification, when an element is referred to as " including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

According to one embodiment of the present invention, the present invention provides a method for the treatment and prevention of a disease caused by a bacterium such as Limonium tetragonum (Thunb.) Bullock , Suaeda malacosperma H. Hara , Boehmeria pannosa Nakai & Satake and Fatsia japonica ) Decne. & Planch ) as an active ingredient. The present invention relates to a cosmetic composition for antioxidation and moisturizing.

The extracts according to the present invention can be obtained through various extraction methods known in the art, and specifically from the group consisting of water, lower alcohols having 1 to 4 carbon atoms, propylene glycol, glycerin and mixtures thereof And may be extracted with a selected solvent.

Specifically, the content of the extracting solvent was found to be in the order of Limonium tetragonum (Thunb.) Bullock , Suaeda malacosperma H. Hara , Boehmeria pannosa Nakai & Satake and Fatsia japonica (Thunb.) Decne. &Amp; Planch ), more preferably 5 to 50 times, and most preferably 10 to 20 times the weight of one or more plant solids.

In addition, the above extract can be extracted at room temperature or warmed under conditions that the active ingredient of the extract is not destroyed or the destruction is minimized. The extraction method is not particularly limited, and for example, cold extraction, ultrasonic extraction, supercritical extraction, ultra-high pressure extraction, reflux cooling extraction, room temperature extraction, hot water extraction and the like can be used depending on the kind of extract and the extraction method.

The filtration is a process of removing suspended solid particles from the extract, and may be performed by filtering particles by using cotton, nylon, paper or the like, or by using an ultrafiltration method, a freezing filtration method, a centrifugal separation method, or the like.

The step of concentrating and then drying the filtrate includes, but is not limited to, freeze drying, vacuum drying, hot air drying, spray drying, vacuum drying, foam drying, high frequency drying, infrared drying and the like. In some cases, a step of pulverizing the finally dried extract may be added.

According to one embodiment of the present invention, the extract may be processed into various forms such as an extract, a powder dried with the extract, and a concentrate obtained by concentrating and filtering the extract.

According to one embodiment of the present invention, there is provided a method for producing a plant of the present invention, comprising the steps of: extracting a plant from the above-mentioned method, such as Limonium tetragonum (Thunb.) Bullock , Suaeda malacosperma H. Hara , Boehmeria pannosa Nakai & Satake , Fatsia japonica (Thunb.) Dec. & Planch ) may be contained in an amount of 0.001 to 20 wt%, more preferably 0.1 to 1 wt%, based on the total weight of the composition. When the amount of the extract is less than 0.001% by weight based on the total weight of the composition, the function as a composition is poor because antioxidation and moisturizing effects can not be sufficiently obtained. When the extract is more than 20% by weight, It is not only economical but also lowers the stability and spreadability of the formulation and is difficult to use as a cosmetic product.

The extract is characterized by having DPPH scavenging activity and hyaluronic acid producing activity. That is, since antioxidation and moisturizing effect can be given at the same time, not only skin beauty but also fundamental skin health improvement can be realized.

As described above, since the extract has excellent antioxidative and moisturizing effects, it can be used as a composition for antioxidation and moisturizing.

The composition for antioxidation and moisturizing can be used as a cosmetic composition, a pharmaceutical composition or a food composition, but it is most preferably used as a cosmetic composition.

According to one embodiment of the present invention, when used as the cosmetic composition, it can be used as an external ointment for skin, a cream, a softening longevity, a convergent lotion, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, Lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutritional cream, eye cream, moisturizing cream, hand cream, foundation, nutrition essence, sunscreen, soap, skin lotion, But are not limited to, any of the formulations selected from the group consisting of cleansing foams, cleansing lotions, cleansing creams, cleansing waters, powders, body lotions and body cleansers. The composition of each of these formulations may contain various kinds of bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide, .

In the case where the formulation of the present invention is bar diesel, in addition to the above-mentioned effective ingredients, there may be used carbomers, triethanolamine, menthol, ethanol, glycerin, disodium iodide, methylparaben, Can be used. Herein, the carbomer and triethanolamine function as a gradual agent, the menthol functions as a fragrance component, the ethanol functions to give a cooling sensation, the glycerin serves as a moisturizing agent, and the disodium is dithiene, And methylparaben is added for its function as an antiseptic. In addition, the fragrance may include various fragrances such as herb fragrance in addition to menthol fragrance.

In addition to the above-mentioned specific ingredients, it is also within the scope of the present invention to apply the known substances widely used in the art to achieve the functions of the thickener, refreshing feeling, fragrance ingredient, .

When the formulation of the present invention is a powder or a spray (spray), lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be added as a carrier component, Propellants such as carbon, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is added as a carrier component. Examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid esters of sorbitan, and the like.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

According to one embodiment of the present invention, the cosmetic composition for antioxidation and moisturizing may be prepared from compositions such as bar diesel, body lotion, body cream, body oil and the like for accelerating fat decomposition with addition of usual additives, Can also be produced. In this case, it is preferable that the cosmetic composition is used as a transdermal administration method in which it is directly applied or sprayed on the skin.

According to one embodiment of the present invention, the amount of the cosmetic composition for antioxidation and moisturizing can be appropriately adjusted according to individual differences, such as the age and condition of the skin fat, the form and the form, and is useful as a composition for antioxidative and moisturizing effects Lt; / RTI >

The composition obtained from the above method exhibits DPPH scavenging activity and hyaluronic acid-producing effect, thus providing excellent antioxidative and moisturizing cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not to be construed as limiting the scope of the present invention. It will be self-evident.

≪ Example 1 >

50 g of Rhodophyceae (Jeju Natural Resources Research Institute) was added with 1 L of 75% ethanol aqueous solution and extracted at 90 ° C for 4 hours. The mixture was filtered through a filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered extract.

≪ Example 2 >

50 g of Glycyrrhizae (Jeju Natural Resources Research Institute) was added with 1 Lg of 75% aqueous ethanol solution and extracted at 90 캜 for 4 hours. The mixture was filtered through a filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered watery plant extract.

≪ Example 3 > Preparation of royal wilt extract

Fifty grams of Royal Mossypool (Jeju Natural Resources Research Institute) was added with 1 L of 75% ethanol aqueous solution and extracted at 90 ° C for 4 hours. The mixture was filtered through filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered royal palm extract.

Example 4: Preparation of a falcon extract

50 g of FALSONI (Jeju Natural Resources Research Institute) was added 1 L of 75% ethanol aqueous solution and extracted at 90 캜 for 4 hours. The extract was filtered through a filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered falcone extract.

Example 5: Preparation of plant complex extract

1: 1: 1 were mixed with each other at a ratio of 1: 1 to 1: 1, followed by mixing 50 g of the mixture (50 g) Was added 1 Lg of a 75% aqueous ethanol solution and extracted at 90 캜 for 4 hours. The mixture was filtered through filter paper, and the extraction process was repeated twice. The filtrate was concentrated under reduced pressure to prepare a final powdered plant complex extract.

<Experimental Example 1> Cytotoxicity test: MTT assay

Cytotoxicity tests were carried out using keratinocytes (HaCaT cells) to determine whether the extracts of Examples 1 to 5 had irritation to cells.

Human-derived keratinocytes (HaCaT cells) were purchased from the American Type Culture Collection (ATCC) and cultured in DMEM medium (Dulbecco's Modified Eagle's Medium, HyClone Lab., USA) supplemented with 10% fetal bovine serum (Gibco BRL, USA) ) At 37 ° C under 5% CO ₂ . The cultured HaCaT cells were dispensed into 96-well plates at 1 × 10 ⁴ cells / mL per well and cultured for 24 hours. After the cultivation, the extracts were cultured for 24 hours by replacing the DMEM medium containing no diluted extracts of each concentration. After 24 hours, CCK-8 (Cell Counting Kit, Dojindo, Japan) solution was added at 1/10 of the cell culture medium, followed by incubation at 37 ° C for 2 hours and then absorbance was measured at 450 nm using a microplate reader . Experiments were carried out three times each, expressed as mean value ± standard deviation, and were calculated using the following equation (1).

[Equation 1]

Cell viability (%) = 100- (absorbance of each sample / control absorbance) x 100

division Cell viability (%) 0 μg / mL 2 μg / mL 10 μg / mL 20 μg / mL 50 μg / mL 100 μg / mL Example 1 100 - 95.54 + 9.19 96.99 + - 4.39 89.67 + - 4.86 91.56 +/- 1.80 Example 2 100 - 103.01 + - 16.17 102.07 ± 17.67 99.39 + 9.03 100.61 + - 4.39 Example 3 100 86.59 ± 0.88 81.30 ± 2.02 71.45 + 1.62 66.87 ± 1.08 69.56 ± 2.53 Example 4 100 - 88.68 ± 5.93 88.24 7.58 12.66 + - 0.85 13.25 + - 0.57 Example 5 100 - 90.21 + - 3.93 95.13 + - 2.18 91.69 ± 1.98 90.59 ± 3.17

As a result, as shown in Table 1, Examples 1 and 2 showed no cytotoxicity up to a concentration of 100 μg / mL, Example 3 showed a cytotoxicity at a concentration of 10 μg / mL or more, Example 4 showed a cytotoxicity . On the other hand, Example 5, which is a plant mixed extract, showed no cytotoxicity up to a concentration of 100 μg / mL and thus it could be used as a safe material for cosmetics. Therefore, experiments were conducted with the concentrations of 100 μg / mL in Examples 1 and 2 and Example 5, 2 μg / mL in Example 3, and 20 μg / mL in Example 4 as the highest concentration.

<Experimental Example 2> Antioxidant test: DPPH scavenging activity

Free radical scavenging activity was evaluated in order to examine the antioxidative effects of the plant extracts of Examples 1 to 5.

The free radical scavenging activity was a modification of the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299-303, 1993). The stable free radical DPPH (1,1-diphenyl-2-picryl hydrazyl , Sigma) reagents were used. First, 1 mL of 0.2 mM DPPH solution was diluted to a suitable concentration in methanol, 2 mL was mixed, and incubated at room temperature for 10 minutes. Absorbance was measured at 517 nm using a microplate reader (Synergy, BioTek, USA). On the other hand, the control group in the free radical scavenging test was measured by the same method except that methanol was added in place of the sample solution. Methanol was added instead of the DPPH solution to obtain a color correction value for each. Experiments were carried out three times each, expressed as mean ± standard deviation, and the free radical scavenging rate was calculated using the following equation (2).

&Quot; (2) &quot;

Free radical scavenging rate (%) = (absorbance of each sample / control absorbance) x 100

division Erase rate (%) 62.5 μg / mL 125 μg / mL 250 μg / mL 500 μg / mL 1000 μg / mL Example 1 33.83 ± 1.98 50.69 + 1.34 77.80 0.95 85.31 + - 0.54 84.47 + - 0.52 Example 2 10.79 ± 1.15 16.60 ± 1.05 28.62 + - 0.56 45.99 + - 0.82 78.01 0.08 Example 3 50.49 + - 0.58 79.76 ± 0.32 80.65 + 0.18 81.59 ± 0.12 83.09 ± 1.47 Example 4 17.59 ± 2.29 42.88 + - 4.08 74.76 ± 0.88 77.11 + - 2.40 81.80 ± 1.81 Example 5 29.11 ± 1.05 52.98 + - 2.33 70.39 ± 1.66 80.21 + - 3.52 85.38 + - 0.98

As a result, as shown in Table 2, all of the plant extracts of Examples 1 to 5 showed a good antioxidative effect, and the antioxidative effect was superior to that of the plant mixed extract of Example 5.

<Experimental Example 3> Moisture Efficacy Test: Measurement of Hyaluronic Acid Production Effect

To evaluate the moisturizing effects of the plant extracts of Examples 1 to 5, the effect of hyaluronic acid production was measured.

First, the cultured HaCaT cells were dispensed into a 24-well plate at 1 × 10 ⁵ cells / mL per well and cultured for 24 hours. After the cultivation, the extracts were cultured for 24 hours by replacing the DMEM medium containing no diluted extracts of each concentration. The amount of hyaluronic acid was determined by measuring the amount of hyaluronic acid using a hyaluronic acid measurement kit (Hyaluronan ELISA kit, Echelon Biosciences, USA). The absorbance of the cell culture without treatment of the sample was taken as 100% of the control, and the experiment was carried out three times each, and expressed as the mean value ± standard deviation.

division Hyaluronic acid (%) 0 μg / mL 2 μg / mL 20 μg / mL 100 μg / mL Example 1 100 - - 125.57 ± 19.36 Example 2 100 - - 148.58 ± 12.87 Example 3 100 129.45 + - 22.42 - - Example 4 100 - 121.81 + - 11.89 - Example 5 100 - - 150.12 ± 9.08

As a result, as shown in Table 3, all of the plant extracts of Examples 1 to 5 were excellent in hyaluronic acid-producing effect, but the hyaluronic acid-producing effect was superior to that of the plant mixed extract of Example 5, Respectively.

<Experimental Example 4> Evaluation of skin moisturizing effect and skin irritation

For the skin moisturizing effect and skin irritation evaluation, the plant complex extract of Example 5 having excellent efficacy was tested based on the evaluation results of the above Experimental Examples. The composition containing the plant complex extract of Example 5 was prepared as a cream type composition according to a conventional preparation method as shown in Table 4 below, and a sensory test was conducted using the same.

ingredient Formulation Example
(weight%) Comparative Formulation Example
(weight%) One The plant complex extract of Example 5 5.0 - 2 Cetearyl alcohol 1.0 1.0 3 Glyceryl stearate / phage-100 stearate 1.0 1.0 4 Polysorbate 60 1.0 1.0 5 Sorbitan sesquioleate 0.3 0.3 6 Cetyl octanoate 6.0 6.0 7 Squalane 8.0 8.0 8 Apricode kernel oil 4.0 4.0 9 Dimethicone 2.0 2.0 10 glycerin 5.0 5.0 11 Magnesium aluminum silicate 0.4 0.4 12 Xanthan gum 0.03 0.03 13 Preservatives and incense a very small amount a very small amount 14 Purified water (to 100) to 100 to 100

First, 20 women over 20 years of age were given cream (test group) containing the composition on the left side of the face and creams (control group) containing no composition on the right side of the face twice daily for 12 weeks . The test was conducted on the subjective efficacy of the skin moisturizing effect. Evaluation of skin irritation was evaluated for skin itching, stinging and erythema. The evaluation was carried out according to the blotting law standards of excellent (5 points), excellent (4 points), normal (3 points), poor (2 points) and very poor (1 point).

number Skin irritation Moisturizing effect Formulation Example Comparative Formulation Example Formulation Example Comparative Formulation Example One 4 4 4 5 2 5 4 5 5 3 4 5 3 3 4 5 5 4 3 5 5 4 5 4 6 5 5 5 4 7 4 4 4 4 8 5 4 5 4 9 4 4 4 5 10 5 5 5 5 11 4 5 5 4 12 5 5 4 3 13 5 5 5 4 14 5 5 5 5 15 5 4 4 4 16 5 5 5 5 17 5 4 5 5 18 5 5 5 4 19 4 5 3 3 20 4 5 5 4 Average 4.65 4.60 4.50 4.15

As a result, as shown in Table 5, it was confirmed that the formulation example was excellent in skin safety due to a low degree of skin irritation as compared with the comparative formulation and showed excellent moisturizing effect.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereto will be. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

Plant extracts of Limonium tetragonum (Thunb.) Bullock , Suaeda malacosperma H. Hara , Boehmeria pannosa Nakai & Satake and Fatsia japonica (Thunb. Decne. & Planch ) A cosmetic composition for antioxidant and moisturizing which is contained as an active ingredient.
The cosmetic composition for antioxidative and moisturizing according to claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, lower alcohol having 1 to 4 carbon atoms, propylene glycol, glycerin and a mixed solvent thereof.
The cosmetic composition for antioxidative and moisturizing according to claim 1, wherein the extract is contained in an amount of 0.001 to 20% by weight based on the total weight of the composition.
The cosmetic composition for antioxidative and moisturizing according to claim 1, wherein the extract has DPPH scavenging activity and hyaluronic acid producing activity.
A cosmetic comprising the composition of any one of claims 1 to 4.