KR100910699B1 - Process for the preparation of red ginseng of which ginsenosides are increased by 9-times steam-heating and 9-times lyophilizing raw ginseng - Google Patents

Abstract

The present invention provides a method for producing red ginseng with increased content of ginsenosides by 9 times of steaming and 9 times lyophilization, and a red ginseng prepared by this method. The prepared red ginseng has a very high content of ginsenosides as an active ingredient while maintaining the intrinsic taste and aroma of ginseng, is very easily dissolved in water and has an excellent function of taking.

Ginsenoside, red ginseng, 9 out of 9 width, freeze dried, freeze dried

Description

Process for the preparation of red ginseng of which ginsenosides are increased by 9-times steam- heating and 9-times lyophilizing raw ginseng}

1 is the standard data of Rg1 and Rb1 of the red ginseng of the present invention in the first experiment,

2 is sample data of Rg1 and Rb1 of red ginseng of the present invention in the first experiment,

Figure 3 is the standard data of Rg1 and Rb1 of the general dried red ginseng of the first experiment,

4 is a sample data of Rg1 and Rb1 of general dried red ginseng of the first experiment,

5 is the standard data of Rg1 and Rb1 of the red ginseng of the present invention in the second experiment,

6 is sample data of Rg1 and Rb1 of red ginseng of the present invention in the second experiment;

FIG. 7 shows standard data of Rg1 and Rb1 of general dried red ginseng in the second experiment,

8 is sample data of Rg1 and Rb1 of general dried red ginseng of the second experiment,

9 is standard data of Rg1 and Rb1 of red ginseng of the present invention in the third experiment.

Figure 10 is the sample data of Rg1 and Rb1 of the red ginseng of the present invention of the third experiment

FIG. 11 shows standard data of Rg1 and Rb1 of general dried red ginseng of the third experiment,

Figure 12 shows the sample data of Rg1 and Rb1 of the general dried red ginseng of the third experiment.

The present invention provides a method for producing red ginseng with increased content of ginsenosides by 9 times of steaming and 9 times of lyophilization and red ginseng prepared by the method.

Ginseng is a perennial root mythology belonging to the Ogapi family according to the food classification, and about 11 species are known on the earth. (1) Korean ginseng (Panax ginseng CA Meyer) is located in the Far East of Asia (33-48 ° North: It is native to some parts of Russia and has excellent efficacy. (2) American Ginseng Panax quinquefolium L. grows and grows in Canada, USA, and (3) Panax notoginseng FH is grown in southwestern region of Guangxi province in southeastern Yunnan Province, China. Wild or cultivated in (4) Panax ginseng Panax japonicus CA Meyer) is known to spread to Japan, southwest China, and Nepal. (和 漢 藥 百科 圖鑑, vol. 1, p. 8, Nambatsunehigui, Hoikusa, 1980).

Ginseng is not only stored as a commodity in the new agricultural plant, but has been used as a valuable medicine since ancient times. As a nourishing tonic, ginseng has undergone many pharmacological and physiological studies on its composition, efficacy, and its mysterious efficacy has been demonstrated and explained by modern scientific methods. The effects of ginseng are various, including aging inhibition, arteriosclerosis prevention, immune function enhancement, thrombosis improvement, brain donation improvement, brain cell protection, anticancer effect, etc. It plays a good role in maintaining homeostasis of human body and changing all pathologies to normal state. (和 漢 藥 百科 科, Vol. 1, Nambatsune Higu, Hoiku-ji Temple, 1980).

In general, ginseng products are divided into cultivated ginseng, white ginseng dried at room temperature, or red ginseng produced by heating the ginseng at 98-100 ° C, and ginseng itself is used for tissue culture. Attempts are being made, but this is a cumbersome process and uneconomical, as it differs significantly from the original ginseng component and requires a process of tissue culture. In addition, Korean Patent Publication No. 1997-239 (published on Jan. 21, 1997) shows that ginsenosides Rg3 and Rg5 treated with ginseng at 120 ° C. to 180 ° C. in order to manufacture processed ginseng with enhanced efficacy have been disclosed. Although ginseng composition containing is disclosed, these methods are a method of preparing low-molecular ginseng composition or ginseng side Rg3, Rg5, etc. in a large amount obtained by acid treatment or heat treatment of ginseng to obtain a large amount of metabolites of a particular saponin. There is a limit.

While ginseng (or ginseng) can be preserved for a long time, it has strong medicinal properties and red ginseng has been attracting much attention due to the identification of specific trace components. Red ginseng is generally produced at 98 to 100 ° C. for about 2 hours, and is widely known to have excellent efficacy compared to white ginseng. In particular, the trace components contained in red ginseng are produced during the manufacturing process of red ginseng by heating, and it is believed that the unique medicinal effects of red ginseng originate from the minor components of red ginseng. Trace components identified to date include ginsenosides such as ginseng saponins such as Rh2, Rs1, Rs2, Rg3, Rg5, Rg2, Rf, Re, Rd, Rg2, Rb2, Rh1, and polyacetylene compounds such as panaxytriol. There is this. Among the trace components of red ginseng, ginsenoside Rg3 inhibits cancer cell metastasis, inhibits platelet aggregation and antithrombotic activity, experimental anti-inflammatory effect, vasorelaxation, and anti-cancer drug resistance. Coagulation inhibition, antithrombin, acid activating action, memory loss improvement, smooth muscle cell growth inhibition, acetylcholine induction, catecholamine secretion and intracellular calcium influx, ginsenoside Rg enhances immune function, platelet aggregation inhibition , Antithrombin, gland activation, memory and learning function, corticosteroid receptor activation (rat brain tissue), protein synthesis, anti-fatigue, antistress, central excretion, vasodilation (relaxation), Defense against harmful stimuli such as high temperature environment and endogenous pyrogens, improvement of stress sexual behavior disorders, anti-nephritis, renal blood flow increase, neurons Promotes survival rate, cholesterol metabolism promotion, anti-inflammatory action, hepatocellular proliferation and DNA synthesis promotion, hepatoprotective action, adrenal cortex stimulating hormone promotion, ginsenoside Rf inhibits pain, inhibits lipid peroxidation, alcohol induction Ginsenoside Re acts as a defense against brain development, Ginsenoside Re stimulates adrenal cortex stimulating hormone, DNA, RNA, protein, lipid synthesis of bone marrow cells, inhibits smooth muscle cell proliferation, protects liver injury, promotes metabolism of cholesterol Cenoside Rc promotes analgesic, PGI2 production, RNA synthesis, protein, and lipid synthesis. Ginsenoside Rb2 promotes sugar, fat metabolism, antidiabetic, epinephrine-induced lipolysis, and cancer toxin hormone. Antagonism, smooth muscle cell proliferation inhibition, tumor clotting silstod inhibition, stress appetite reduction, promotion of antioxidant active substance production, liver tissue ATP content Ginsenoside Rb1 is a central antagonist, psychostable, anticonvulsant and analgesic, adrenal cortical stimulating hormone secretion, anti-anxiety, triglycerides and free fatty acids, protein synthesis, neuronal cell survival Action, acetylcholine release promotion, memory improvement, platelet aggregation inhibitory action, lipid peroxidation inhibitory activity, carbon tetrachloride induction action, cholesterol metabolism promotion action, etc. Ginnoside Rh1 promotes tumor cell differentiation, platelet aggregation, etc., Ginnoside Rh2 has been shown to have specific effects such as inhibiting cancer cell proliferation, promoting the regeneration of cancer cells, inhibiting cancer cell infiltration, inhibiting tumor growth, and increasing anticancer activity of anticancer agents.

Ginsenoside Rb (diol) group has central inhibitory effect, Ginsenoside Rg (triol) group has hemolytic inhibition as opposed to hemolysis, which is a general property of saponin, and Ginsenoside Rh, g group. (群) has a hemolytic action is antagonizing each other.

references :

J. Nat. prdo, 63, 1702, (2000),

Biol. Pharm. Bull. 21, 79, (1998),

Korean, J. Ginseng Sci., 21, 132 (1997),

J. Neuroscience, Res. 53, 426 (1998),

Neuro Report 9, 226 (1998),

Jpn, Cancer, Res, 87, 357 (1996),

J. Cancer Rcs, clin, Oncol. 120, 24 (1993),

Anticancer, Rsc, 15, 1067 (1997) Cancer Letter 159, 41 (2000),

Deitaru Anticarcinogenesis and Antimutagenesis, 274 (2000),

J. Natural Product, 3, 1702 (2000),

Patent registration No. 192678.

In the conventional red ginseng manufacturing method, steamed ginseng is dried and then manufactured, and the yield of high-quality red ginseng is about 12%. This is because the pore occurrence rate is about 46% and the incidence of cracking is high at 23%. . It is known that the production of good red ginseng is related to starch swelling force of ginseng itself and starch swelling force to core temperature. In the future, a lot of research is required, and steam ginseng is recommended to be steamed below 98 ℃, especially 94 ~ 97 ℃ in steam ginseng, and this method suppresses the loss rate of natural ingredients, It can be said that there are a lot of problems in maintaining the odor, the active ingredient as it is. In addition, there is a small number of disadvantages that the smell of ginseng is lost by the heating process for a long time and the main ingredient is destroyed. The present invention is provided as a condition of suppressing steam divergence during steaming as much as possible, steaming 9 times, introducing a unique freeze-drying method, drying 9 times, the inherent pharmacology of the composition, inherent taste, flavor, and active ingredients The effect can be expected, and if necessary, the raw ginseng is pulverized into 80 mesh, powdered and leached in hot water, and when necessary, lactose or excipients are added to increase the composition, flavor, and flavor of the ginseng. However, when the patent application is disclosed or the patented publicly known technology, conventionally washed ginseng is put into steam ginseng and steamed at 94 to 100 ° C., in particular, about 96 ° C. using steam, and dried or effective in ginseng extract. In order to extract only the ingredients, a method of extracting impurities and precipitates by extracting them with an appropriate solvent, concentrating them, and then concentrating them through a drying process have been developed. No method has been developed for the preparation of red ginseng.

The present invention performs the steaming and drying process of 9 to 9 widths rather than the usual method in the ripening-drying process of ginseng, especially the ginseng-specific gin described above by adopting the freeze drying method in the whole drying process, especially the final process. To maximize the pharmacological effects of senoside Rg3, and the like, to preserve the taste, flavor, and the like of ginseng, as it is to provide red ginseng that can be easily taken by drinking as it is, or immersed in water.

The present invention is to provide a method for producing red ginseng by steaming the ginseng (or wild ginseng) nine times and freeze-dried nine times (until the remaining moisture is about 14%). The present invention also provides a red ginseng prepared by steaming nine times (or wild ginseng) nine times and lyophilizing nine times (until the remaining moisture is about 14%).

General Example:

In the present invention, two conditions were set to confirm the change in the effective ingredient. In other words, ginseng (or ginseng, wild ginseng) is washed and ginseng (or Brissan ginseng) prepared by the following freeze-drying ship after concentration of about 2 hours (7 Brix) through an extraction process (Extraction Process) at 100 ℃ for 3 hours. Changes in partial structure and content of ginsenosides of ginseng and wild ginseng) were confirmed.

Freeze drying condition

    4 hours 0 ° C rise from -40 ° C

      15 hours hold at 0 ℃

      3 hours increase from 0 ℃ to 10 ℃

      7 hours at 10 ℃

      2 hours 20 ° C rise from 10 ° C

      7 hours at 20 ℃

     7 hours 40 ° C rise from 20 ° C

      10 hours at 40 ℃

Another is to wash the ginseng (or fresh ginseng, wild ginseng) that is the core of the present invention by increasing the ginseng (110 ℃, 3 hours) and then freeze-dried under the following conditions and then freeze-dried again after steaming (100 ℃, 3 hours) This is a method of manufacturing until the remaining moisture is 14% by repeating a total of nine times. First, the red ginseng-dry is increased to 100 ° C. for 3 hours and dried by freeze drying condition (A) method, and the second is the red ginseng-drying to 110 ° C. for 3 hours to freeze drying condition (B) method. Third, freeze-drying conditions (A) method by increasing the ginseng-drying to 100 ℃, 3 hours to the third, freeze-drying conditions (B) method by increasing the ginseng-drying to 110 ℃, 3 hours, Fifth is the freeze drying condition (A) method by increasing the ginseng-drying to 100 ℃, 3 hours, and 6th is the freeze-drying condition (B) method by increasing the ginseng-drying to 110 ℃ and 3 hours. Is the freeze-dried condition (A) method by increasing the ginseng-drying to 100 ℃, 3 hours, the eighth is the freeze-drying condition (B) method by increasing the ginseng-drying to 110 ℃, 3 hours, and finally the ninth Is steamed 9 times and freeze dried 9 times by freeze drying condition (A) The tread is manufactured.

A Freeze Drying Conditions

    2 hours 0 ° C rise from -40 ° C

      7 hours and 30 minutes at 0 ℃

      1 hour 30 minutes

      3 hours 30 minutes at 10 ℃

     1 hour rise from 10 ° C to 20 ° C

      3 hours 30 minutes at 20 ℃

     3 hours 30 minutes

      5 hours at 40 ℃

B Freeze Drying Conditions

    2 hours 0 ° C rise from -40 ° C

      15 hours hold at 0 ℃

      3 hours increase from 0 ℃ to 10 ℃

      7 hours at 10 ℃

      2 hours 20 ° C rise from 10 ° C

      7 hours at 20 ℃

      5 hours at 40 ℃

Effectiveness of ginsenosides Rg3, Rg2, etc. identified by the preparation method of the present invention when comparing the embodiment of the ginseng extract-concentration-freeze drying under the freeze-drying conditions of the above two drying process and the red ginseng manufacturing process of the present invention It was confirmed that secretion is unusually high.

In the present invention, the red ginseng may also be prepared by steaming ginseng (fresh ginseng or wild ginseng) as a raw material, extracting, freeze-drying, and repeating steaming-freeze-drying to increase the content of ginsenosides.

In the present invention, Sagunjatang (四 君子 湯: ginseng, Baekchul, Baekbongryeong and licorice each prepared by weighing 5g each), Ssanghwatang (雙 和 湯: 10g of Baekjak, Sukjihwang, Hwanggi, Dongguk, Cheonggung each 4g, cinnamon, licorice each 3g Eda 3 pieces of ginger and 2 pieces of coarse rice are added to the decocted soup), and the jeonjeondaebo tang (ginseng, Baekchul, Baekbongryeong, licorice, Sukjihwang, Cheongung, Donkey, Astragalus, broiler) and 3 pieces of ginger. Cut 2 slices into 1 pill, decoction, Bojung Iktang (補中益氣 湯: 6g, yellow ginseng, licorice white extract 4g, donkey ghost, dermis 2g, horse riding, 1.2g each slice 2 slices 복), tang guibo blood boiled tang (當歸 血 補 湯: ang gui horse riding each 6g, hwahwacho, cheongpi, hyungae, baekchul, sukjihwang, 2.8g each, cheongung 2g) , Ginseng Yeongyangtang (人蔘 榮 養 湯: Baekjakju liquor 8g, Angelica, Ginseng, Baekchul, Hwanggi buckwheat, Broiler, Dermis, Licorice each 4g, Sukjihwang, Schisandra chinensis, Insect insect 3g, 2g raw paper 3 pieces and 2 pieces of contrast sauce, Palbohoechuntang (八 補 回春 湯: Baekjak 5g, Astragalus 3,2g, Baekchul 2,4g, Baekboksin, Half each 2g, Rich, Ginseng, Ephedra, Golden, Banggi, Pedestrians, Cheongung, Angelica, Dermis, Windproof, Broiler, Health, Haengja, Sukjihwang, Raw Dried Sulfur, Licorice each 1.6g, Incense, Yakyang, Cheonphu each 1.2g, cut into 1 scoop, 3 gingers and crude 2 In the preparation of a decocted sweetener) and the like, it may be prepared by adding red ginseng having increased content of ginsenosides prepared in claim 1. Here, the herbal ingredients such as Sagunjatang, Ssanghwatang, Sipjeondaebotang, Bogi-Ikgi-tang, Dangwi-Gyeolbo-tang, Ginseng Nutrition Bath, and Palbo Rejuvenation Bath, except for ginseng components, were bathed and then ginsenosides prepared in the present invention. Red ginseng with increased content can be added and lyophilized to produce fine powders that dissolve very well in water.

Hereinafter, the present invention will be described in more detail with examples.

Example 1

After washing ginseng (or fresh ginseng, wild ginseng), steamed ginseng (110 ℃, 3 hours), freeze-dried under the following conditions, then steamed red ginseng (100 ℃, 3 hours), and freeze-dried to make the remaining 9 times. The moisture was dried to 14% to obtain red ginseng.

A Freeze Drying Conditions

    2 hours 0 ° C rise from -40 ° C

      7 hours and 30 minutes at 0 ℃

      1 hour 30 minutes

      3 hours 30 minutes at 10 ℃

     1 hour rise from 10 ° C to 20 ° C

      3 hours 30 minutes at 20 ℃

     3 hours 30 minutes

      5 hours at 40 ℃

According to the first freeze-drying method of ginseng = extraction-freeze drying in the configuration of the present invention, the relative peak area of saponin of ginseng according to sample conditions is shown in Table 1 below.

Table 1.

Example 2

After washing ginseng (or fresh ginseng, wild ginseng), steamed ginseng (110 ℃, 3 hours), freeze-dried under the following conditions, then steamed red ginseng (100 ℃, 3 hours), and freeze-dried to make the remaining 9 times. The moisture was dried to 14% to obtain red ginseng.

B Freeze Drying Conditions

    2 hours 0 ° C rise from -40 ° C

      15 hours hold at 0 ℃

      3 hours increase from 0 ℃ to 10 ℃

      7 hours at 10 ℃

      2 hours 20 ° C rise from 10 ° C

      7 hours at 20 ℃

      5 hours at 40 ℃

When the ginseng-steam-freeze-drying of the second freeze drying method in the configuration of the invention according to the sample conditions of the ginseng saponin relative peak (peak) area is shown in Table 2.

Table 2.

In the above test, the content of the active ingredient (ginsenosides) was very high in the sample prepared by heat-steam-freeze-drying (9 times) rather than extraction.

Experimental Example 1

Ginsenosides Rg1 and Rb1 in red ginseng prepared by the method of Example 1 of the present invention were quantified by the following method.

1. Analysis Conditions

 HPLC: Hitachi L-2130 Series

Column: Phenomenex Luna C18 ODS (2) 4.6x250nm

Wavelength: UV 203nm

Flow rate: 1.0mL / min

Column Temperature: 30 ℃

Injection Volume: 20μl

Mobile Phase:

2. Pretreatment method of standard solution and sample solution

(1) Preparation of standard medicine

About 10 mg of each standard of Ginsenoside Rg1 and Rb1 is dissolved in 10 ml of methanol, and diluted 5 times as standard solution.

(2) Preparation of the test liquid

   end. The red ginseng of Example 1 was ground, about 1 g of which was added, and 150 ml of methanol was extracted and refluxed for 2 hours.

  I. Cool, filter and evaporate the filtrate.

  All. 10 ml of methanol is added to the residue and dissolved.

  la. The solution is filtered using a 0.45 μm filter to prepare a sample solution.

This experiment was repeated three times, and the conventional red ginseng was prepared in the same manner as in the preparation of (2) sample solution and analyzed three times to compare the contents of Rg1 and Rb1.

3. The result of analyzing the content

  The analysis results are shown in Table 3 below.

Figure 1 shows the standard data of Rg1 and Rb1 of the red ginseng of the present invention in the first experiment.

Figure 2 shows the sample data of Rg1 and Rb1 of the red ginseng of the present invention of the first experiment.

Figure 3 shows the standard data of Rg1 and Rb1 of general dried red ginseng of the first experiment.

Figure 4 shows the sample data of Rg1 and Rb1 of general dried red ginseng of the first experiment.

Figure 5 shows the standard data of Rg1 and Rb1 of the red ginseng of the present invention in the second experiment.

Figure 6 shows the sample data of Rg1 and Rb1 of the red ginseng of the present invention of the second experiment.

Figure 7 shows the standard data of Rg1 and Rb1 of the general dried red ginseng of the second experiment.

8 shows sample data of Rg1 and Rb1 of general dried red ginseng of the second experiment.

Figure 9 shows the standard data of Rg1 and Rb1 of the red ginseng of the present invention of the third experiment.

Figure 10 shows sample data of Rg1 and Rb1 of the red ginseng of the present invention of the third experiment.

Figure 11 shows the standard data of Rg1 and Rb1 of the general dried red ginseng of the third experiment.

Figure 12 shows the sample data of Rg1 and Rb1 of the general dried red ginseng of the third experiment.

As confirmed from the above experimental results, it can be seen that the content of Rg1 and Rb1 in the red ginseng prepared by the method of the present invention is much higher than that of the red ginseng prepared by the conventional method of preparing red ginseng.

The red ginseng produced by the manufacturing process of 9 to 9 width of the present invention has a very high content of ginsenosides as an active ingredient while maintaining the intrinsic taste and aroma of ginseng, and is very easily dissolved in water and has an excellent taking function. Has an effect.

Claims (11)

In the method of producing red ginseng with fresh or wild ginseng, steaming fresh ginseng or wild ginseng at 100 ° C to 110 ° C and lyophilizing nine times under the conditions of A or B to increase the content of ginsenosides Method for producing the prepared red ginseng.      A Freeze Drying Conditions:        4 hours 0 ° C rise from -40 ° C        15 hours hold at 0 ℃        3 hours increase from 0 ℃ to 10 ℃        7 hours at 10 ℃        2 hours 20 ° C rise from 10 ° C        7 hours at 20 ℃        7 hours 40 ° C rise from 20 ° C        10 hours at 40 ℃ B Freeze Drying Conditions:        2 hours 0 ° C rise from -40 ° C        15 hours hold at 0 ℃        3 hours increase from 0 ℃ to 10 ℃        7 hours at 10 ℃        2 hours 20 ° C rise from 10 ° C        7 hours at 20 ℃ 5 hours at 40 ℃ Steamed ginseng or wild ginseng at 100 ° C to 110 ° C, extracted, and the extract was freeze-dried under the conditions of freeze-drying conditions A or B of claim 1, and steamed again-freeze-drying to repeat the content of ginsenosides. Method for preparing increased red ginseng extract extract. The method of increasing the content of ginsenosides of Sagunja-tang by adding red ginseng prepared in claim 1 or 2 as its ginseng component to Sagunja-tang. Red ginseng or red ginseng extracted with red ginseng extracted by steaming fresh ginseng or wild ginseng at 100 ° C. to 110 ° C., followed by freeze-drying nine times under conditions A or B.      A Freeze Drying Conditions:       4 hours 0 ° C rise from -40 ° C        15 hours hold at 0 ℃        3 hours increase from 0 ℃ to 10 ℃        7 hours at 10 ℃        2 hours 20 ° C rise from 10 ° C        7 hours at 20 ℃        7 hours 40 ° C rise from 20 ° C        10 hours at 40 ℃       B Freeze Drying Conditions:        2 hours 0 ° C rise from -40 ° C        15 hours hold at 0 ℃        3 hours increase from 0 ℃ to 10 ℃        7 hours at 10 ℃        2 hours 20 ° C rise from 10 ° C        7 hours at 20 ℃        5 hours at 40 ℃  Sagunjatang with increased content of ginsenosides prepared by adding red ginseng or its extract extract to Sagunjatang. Ssanghwa-tang, in which the content of ginsenosides prepared by adding red ginseng or its extract extract to Ssanghwa-tang. A red ginseng or extract extract thereof of claim 4 is added to the jeonseondaebotang to increase the content of ginsenosides jeonseondaebotang. Guaranteed increase in the content of ginsenosides prepared by adding the red ginseng of the claim 4 or its extract extract to the guaranteed guarantee. Dangguiboheoltang of the ginsenosides prepared by adding red ginseng or its extract extract to Dangguiboheoltang. Ginseng nutrient-tang having increased content of ginsenosides prepared by adding red ginseng or its extract extract to ginseng nutrient-tang. Palbohoechuntang with increased content of ginsenosides prepared by adding red ginseng or its extract extract to Palbohoechuntang.

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