KR101392204B1 - Composition comprising herbal extract of red ginseng, Angelicae gigantis Radix, citrus peel and green tea leaves for treating or preventing vascular diseases - Google Patents

Abstract

The present invention relates to a composition for preventing or treating vascular diseases containing red ginseng, Angelica gigas, dermis, and green tea. The composition is excellent in a platelet aggregation inhibiting effect and is useful as a therapeutic agent for atherosclerosis, hypertension, angina pectoris, myocardial infarction, ischemic heart disease, And may be useful as a therapeutic agent for vascular diseases including cerebral infarction, cerebral infarction, cerebral hemorrhage, stroke,

Description

[0001] The present invention relates to a composition for preventing or treating vascular diseases containing herbal extracts of red ginseng, Angelica gigas, dermis, and green tea (Composition comprising a herbal extract of red ginseng, Angelica gigantis Radix, citrus peel and green tea leaves for preventing or preventing vascular diseases,

The present invention relates to a composition for preventing or treating vascular diseases containing herbal extracts of red ginseng, Angelica gigas, dermis and green tea.

In modern society, vascular disease is becoming a serious social problem. According to the statistics released by the National Statistical Office in 2010, vascular diseases including hypertensive diseases, ischemic heart diseases and cerebrovascular diseases are the second leading cause of death in Korea, followed by malignant tumors, Or more, the mortality rate of vascular disease is greatly increased in women over 65 years of age. In particular, risk factors associated with atherosclerosis are age, sex, hypertension, hyperlipidemia, diabetes, smoking, lack of exercise and obesity. Examples of the vascular diseases include arteriosclerosis, hypertension, angina pectoris, myocardial infarction, ischemic heart disease, heart failure, complications arising after pericardial artery arteriography, cerebral infarction, cerebral hemorrhage, and stroke. Patients with vascular disease receive continuous medication to treat it, but it is not easy to cure it in modern medicine. If you take the medicine for a long time, the side effect is big.

On the other hand, for the prevention and treatment of vascular diseases, many herbal medicines are introduced in the traditional books such as the private or Dongbibogam. Among them, Angelica, dandelion, red ginseng, It has been proven.

Red ginseng (red ginseng) is a red ginseng produced by steaming roots of ginseng (ginseng). Ginseng that can be used for the production of red ginseng is a perennial plant belonging to the genus Panax, ginseng , Panax quinquefolia , and Panax notoginseng , Panax vietnamensis , Panax elegatior , Panax wangianus) or non-Panax pinra typhimurium Douce (Panax bipinratifidus), Panax eye styryl poly Titanium (Panax angustifolium ). Red ginseng contains a large amount of saponin. Most saponin present in the plant is oleanane, and red ginseng saponin is triterpenoid saponin of the dammarane family And ginsenoside Rh2, Rg2, Rg3, Rg1, Rh1 and the like as the saponin component peculiar to red ginseng. The general characteristic of saponin is that it is soluble in water, alcohol, it is consistently bubbling, physiologically detoxifying, and is not toxic to overdose. In particular, ginsenoside has been known to exert various effects on the body control function by affecting the central nervous system, the endocrine system, the immune system, and the metabolic system. Red ginseng has pharmacological effects such as nourishing tonic action, immunity enhancing action, and blood sugar lowering, and has excellent effect on vascular disease by lowering cardiac output and central venous pressure. In addition, the anti-cancer effect of the ethanol extract of red ginseng was enhanced by gamma irradiation (Non-Patent Document 1), the effect of fermented red ginseng powder on the serum lipid concentration and antioxidant activity in rats (Non-Patent Document 2) Reaction and Effects on Beta-adrenergic Receptor Function (Non-Patent Document 3) It is known that red ginseng can be widely used in various fields such as medical and functional foods. In addition, red ginseng has been recognized for its functional properties such as 'restoring fatigue', 'improving immunity', 'helping blood flow through inhibiting platelet aggregation', and 'improving memory'. Yu et al. Treated red ginseng extract on platelets of animals showed that platelet aggregation substances induced by platelet aggregation inducers (collagen, arachidonic acid, thrombin, etc.) were dose-dependently inhibited, but activated partial thromboplastin time (APTT) (non-patent document 4). Park et al. have reported that panaxadiol saponins in red ginseng produce thromboxane A2 (TXA2) by thrombin in normal platelets (Non-Patent Document 5). In a double-blind, randomized, double-blind, randomized, double-blind, randomized, double-blind, randomized, double-blind, randomized, double-blind, Patent Document 6). In addition, the study by Lee, Jung-Hee, et al., Who compared long-term ingestion of red ginseng products for 4 to 5 years, showed a significant decrease in platelet aggregation response and a significant increase in blood coagulation time (Non-Patent Document 7).

Angelica (當歸, Angelicae gigantis Radix) Angelica gigas Nakai is (Angelica gigas Nakai) roots are used. In China, Chinese Angelica Sinensis (Oliv.) Diels: 中國 当期) is used in Japan and why Angelica acutiloba (Sieb. & Zuc.) Use Kitagawa). Angelica contains mainly coumarin-based decursin, decursinol, decursinol angelate and the like. Decker Shin and Decker Cynol are known to have sedative, analgesic action, antitumor action, antitumor action, brain cell protective action, and especially anti-platelet aggregation, which is effective for vascular treatment. In one room, Angelicae is used mainly for the recovery of anemia, women's diseases and postpartum of pregnant women. Besides, it is known that it is effective for severe cough and swelling. In addition, it not only strengthens the uterus but also regulates the physiology to remove waste products such as hemorrhoids, improves the poor circulation, improves the color deficiency, helps to recover the prenatal and postnatal blood, In addition, the blood circulation can be smoothly performed, and blood purification and intestinal motions can be actively promoted, thereby improving hand and foot symptoms (Non-Patent Document 8). Studies on Angelica gigas include studies on herbal cosmetics containing Angelica keiskei kana (non-patent document 9), effects on damaged sciatic nerve regeneration of Angelica gigas rat (non-patent document 10), Angelica gigantosa extract and skin aging (Non-patent document 11), studies on the effect of encapsulated Angelica gigasum powder on female hormone changes and osteoporosis (non-patent document 12), etc., .

The dermis (Citrus peel) in Korea is the citrus ( Citrus unshiu Markovich) or the mature periplasm of an in situ relative plant ( Rutaceae excess). In Japan, citrus unshiu Markovich) and byeonggam (Citrus reticulata Blanco: the柑) into the dermis, gyulgam (Citrus tachibana (Makino) Tanaka: 橘 柑) is used as a citrus peel (橘花) in China and the disease ( Citrus reticulata Blanco: 柑) and its cultivar variant as dermis. Dried peaches are flavonoids such as hesperidin, rutin, naringin, neohesperidin and the like, and limonoid-based nomilinic acid and the like , In particular, 4.0% or more of hesperidin. In addition, it contains vitamin B1, vitamin C, calcium, iron, dietary fiber and so on. Among them, the vitamin C is known to play a role of strengthening the skin and the mucous membrane by preventing the body temperature from lowering by smoothly metabolizing, preventing the cold in winter, and the like. The main effects of the dermis include arthropods, windbreaks, and anti-allergic effects, but the main effect is capillary vascular strengthening, which can be used to prevent and treat various vascular diseases such as hypertension, cerebral hemorrhage, and cerebral infarction have. On the other hand, regarding the dermis, the processing characteristics and antioxidant properties of a single leach using a dermis (non-patent document 13), dermis anti-inflammatory effect (non-patent document 15) And research on the antioxidant effect of dermis.

Green tea can be harvested from the tea plantation. The tea plantation originates in the region of India. The components of green tea are phenolic compounds such as catechin, epicatechin, epicatechin gallate, and purine alkaloids such as caffeine, xanthine, . Catechin has excellent antioxidant activity against vitamin C, anti-inflammation, antimutagenic and anticancer effects, anti-hypertensive effect, capillary vascular strengthening effect, antithrombotic effect It is a functional substance known to be helpful for cardiovascular treatment and prevention. Studies on green tea include meta-regression analysis of anti-diabetic effect (non-patent document 16), isolation and structure identification of antimicrobial substance from green tea extract (non-patent document 17), weight loss and epididymal fat And the influence on the accumulation control (Non-Patent Document 18).

On the other hand, Korean Patent Registration No. 512252 (Patent Document 1) discloses that the methanol extract of the herbal composition mixed with the dermis and red gins with respect to the herbal medicine has an anti-thrombotic effect, and the composition containing the red ginseng powder as the main component It is disclosed in Korean Patent Publication No. 2011-0122499 (Patent Document 2) that a food containing Angelica gigantosa extract has an effect of inhibiting platelet aggregation, and it is known that a composition containing red ginseng as an active ingredient is effective in improving blood circulation. Japanese Patent No. 345040 (Patent Document 3) discloses that an extract of a crude drug mixture containing a part of a dermis has been treated in atherosclerosis in Korean Patent No. 787174 (Patent Document 4) , Angelica japonica, Dermis, and Green tea extract have therapeutic effects on vascular diseases.

Accordingly, the inventors of the present invention found that the herbal medicine extracts of red ginseng, angelica gingiva, dermis, and green tea had a superior synergistic effect on the treatment of vascular diseases than the respective herbal medicine extracts, while studying drugs effective for prevention and treatment of vascular diseases The invention could be completed.

Korea Patent No. 512252 (Anti-thrombogenic composition, Announcement of 2005.09.05) Korean Patent Publication No. 2011-0122499 (Method for manufacturing health functional food using red ginseng powder, disclosed on November 11, 2011) Korean Registered Patent No. 345040 (Improvement agent for blood circulation containing red ginseng complex and method for preparing the same, 2002/04/04) Korean Patent No. 787174 discloses an extract for prevention and treatment of arteriosclerosis and related diseases of herbal medicinal herb preparations including Bacillus, Pseudomonas aeruginosa, Chimmae, Dermis, Bepyeong, And pharmaceutical compositions for the prophylaxis and treatment of related diseases, 2002.07.24 Announcement)

Increase of anticancer effect by gamma irradiation of ethanol extracts of Huh Jung-moo, Kim Dong-ho, and red ginseng, Journal of applied biological chemistry, 2011, 54 (1), 15-20 Jae Young et al., Effects of Red Ginseng Fermented Red Ginseng Powder on Serum Lipid Concentration and Antioxidant Activity in Rats, Korean Journal of Food Science and Nutrition, 2009, 38 (9), 1153 ~ 1160 The effect of red ginseng on stress response and beta-adrenergic receptor function in normal people, such as Shin Woo Yong, Korean Journal of Psychopharmacology, 2009, 20 (3), 135 ~ 140 Yu J. Y. et al., Antiplatelet and antithrombotic activities of Korean red ginseng, Arch. Pharm. Res., 2006, 29, 898-903 Park H. J. et al., Panaxadiol from Panax ginseng C.A. Meyer inhibits synthesis of Thromboxane A2 in platelet aggregation induced by thrombin, Korean J. Ginseng Sci., 1993, 17 (2), 131-134 Effect of red ginseng concentrate on blood circulation in healthy volunteers: randomized, double-blind, placebo-controlled trial, Korean Ginseng Journal, 2007, 31, 109 ~ 116 Lee, JH, Kim, SJ, Effects of long-term ginseng intake on human thrombotic factors, Korean Nutrition Society, 1995, 28, 862 ~ 871 Compilation committee on herbal medicine textbooks, Pharmacology, 2003 Kwon, Hye-jung, and Hwangjang-tang, a study on herbal cosmetics, The effect of red ginseng on the damaged sciatic nerve regeneration in rats, Korean Journal of Oriental Medicine, 2008, 29 (2), 133 ~ 150 Yoo Mi-ae, Janggi extract, and dexakin on skin aging inhibition efficacy, Ajou University doctoral dissertation, 2011 Choi, Kyung-Ok, Effects of encapsulated Angelicae radix powder on female hormone changes and osteoporosis, Sejong University, Master's thesis, 2011 Kim, Hye-kyung, et al., Confirmation of antitoxicidal activity of dandruff of oriental herbal medicine, Journal of Physiology and Pathology, 2008, 22 (1), 96 ~ 99 Yoo, Kyungmi et al., Processing Characteristics and Antioxidant Properties of Single Leachate Using Jeju Dumpling, Korean Journal of Food Science and Technology, 2005, 21 (3), 354 ~ 359 A Study on the Anti-inflammatory Effects of Jeong Sung-Woong and Dermis, Doctoral Thesis, Kyung Hee University, 2009 Yoon JH, Choi KH, Meta-regression analysis of anti-diabetic effects of green tea, Korean Journal of Data Science, 2011, 22 (4) 717 ~ 726 Shin, S., et al., Isolation and Structure Identification of Antimicrobial Substances from Green Tea Extracts, Korean Journal of Food Preservation, 16 (6), 2009, 924 ~ 928 Park, Pil-joon, et al., Effects of hot water green tea extract on the control of body weight and epididymal fat accumulation in mice, Korean Journal of Food Science and Technology 42 (1), 2010, 103-108 Son D.J. et al., Antiplatelet effect of green tea catechins: a possible mechanism through arachidonic acid pathway. Prostaglandins, Leukotrienes and Essential Fatty Acids, 2004, 71, 25-31 Born G.V. et al., The aggregation of blood platelets. Journal of Physiology 1963, 168, 178-195

It is an object of the present invention to provide a composition for preventing or treating vascular diseases containing herbal extracts of red ginseng, Angelica gigas, dermis and green tea.

The present invention relates to a composition for preventing or treating vascular diseases containing herbal extracts of red ginseng, Angelica gigas, dermis and green tea. The vascular disease may be a disease selected from the group consisting of arteriosclerosis, hypertension, angina pectoris, myocardial infarction, ischemic heart disease, heart failure, complications arising after cardiovascular artery arthroplasty, cerebral infarction, cerebral hemorrhage, and stroke.

The herbal extract of red ginseng, Angelica gigas, dermis and green tea of the present invention can be prepared by using any extraction method commonly used, and for example, immersion (cold-rolling, warming), hot water extraction, ultrasonic extraction, However, the present invention is not limited thereto. The herbal medicine extracts of red ginseng, Angelica gigas, dermis and green tea can be prepared by extracting with a conventional extraction solvent selected from among water, alcohol aqueous solution and alcohol. These extracts can be used alone or in combination with other acceptable herbal medicines . The alcohol may be a lower alcohol having 1 to 4 carbon atoms, preferably ethanol, methanol, propanol, isopropanol, and butanol.

The herbal medicine extracts of red ginseng, Angelica gigas, dandelion, and green tea may be an extract of a herbal medicine mixture in which the red ginseng, Angelica gigas, dermis, and green tea are mixed at a weight ratio of 2: 6: 1 to 2: 1: 1. Preferably, the extract is prepared by adding an extracting solvent of 5 to 15 times the weight of the herbal mixture to a herbal mixture mixed with a weight ratio of 2: 6: 1 to 2: 1: 1 of red ginseng, Angelica jezoensis, And the mixture is extracted at a temperature of 40 to 120 ° C for 2 to 24 hours, preferably 4 to 12 hours, filtered, and dried and pulverized to obtain a filtrate. In addition, the extraction process can be repeated one to three times. The extraction solvent may be selected from water, an aqueous solution of an alcohol, and an alcohol.

The extract of the present invention can be dried using conventional drying methods such as vacuum drying, spray drying, or freeze drying.

Therefore, preferably, the extract of the present invention contains

(1 step) adding an extracting solvent of 5 to 15 times the weight of the herbal mixture to the herbal mixture mixed with the weight ratio of 2: 6: 1 to 2: 1: 1 of red ginseng, Angelica jezoensis, dermis and green tea;

(Step 2) Extracting the mixture of the crude drug mixture and the extraction solvent at a temperature of 40 to 120 ° C for 2 to 24 hours and filtering to obtain a filtrate; And

(Step 3) Drying and pulverizing the filtrate.

Meanwhile, the extraction solvent used in the first step may be added in the same amount to the solid material from which the filtrate is removed in the above two steps, and then the extraction and filtration steps of the two steps may be repeated, followed by the third step. The extraction and filtration may be repeated one to three times.

The content of the extract as an active ingredient in the composition for preventing or treating vascular diseases containing the herbal medicine extract of red ginseng, angelica, dermis, and green tea according to the present invention can be appropriately controlled according to the use form and purpose, the severity of the patient, May be 0.01 to 99.9% by weight, preferably 0.1 to 50% by weight, based on the weight of the solid content, but is not limited thereto.

In addition, the above composition may be administered in various formulations of oral and parenteral administration in actual clinical administration. In the case of formulation, a diluent or excipient such as a filler, a weight agent, a binder, a wetting agent, a disintegrant, Can be used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient, such as, for example, , Starch, celluloses, calcium carbonate, crospovidone, light silicic anhydride, lactose, magnesium stearate, talc, enzyme-treated stevia, microcrystalline cellulose, magnesium stearate, gelatin and the like. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration may include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, and freeze-drying agents. Examples of the non-aqueous solvent and suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.

The dose of the composition containing the extract of the present invention may be varied depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate and severity of disease, It may be administered once to several times a day at a predetermined time interval. For example, the daily dose may be 0.001 to 500 mg / kg, preferably 0.1 to 200 mg / kg, based on the active ingredient.

In another aspect, the present invention provides a health functional food for preventing or improving vascular diseases containing herbal extracts of red ginseng, Angelica gigas, dermis, and green tea. The health functional food may be various foods, beverages, food additives, and the like.

The content of the extract as an active ingredient contained in the health functional food may be appropriately determined depending on the form of the food and the intended use, and may be, for example, 0.01 to 50% by weight of the total food weight, 0.1 to 10% by weight. The health drink composition may be added at a ratio of 0.02 to 10 g, preferably 0.1 to 5 g, based on 100 ml.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates such as ordinary beverages as an additional ingredient, as long as it contains the extract as an essential ingredient at the indicated ratio, and there is no particular limitation to the liquid ingredient. Examples of the above-mentioned natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose, polysaccharides such as maltose, sucrose and the like, such as dextrin, cyclodextrin and the like And sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above . The ratio of the natural carbohydrate is generally 1 to 20 g, preferably 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above-mentioned composition, the composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and intermediates (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the compositions of the present invention may contain flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0.001 to 20 parts by weight per 100 parts by weight of the composition of the present invention.

In particular, red ginseng, Angelica gigas, dandruff and green tea, which are raw materials for the extract of the present invention, have been used in herbal medicine for a long time. When administered to human body, there is no worry about side effects compared with other synthetic drugs. The toxicity test for standardized herbal composition was found to have no effect in vivo.

As described above, herbal medicine extracts of red ginseng, Angelica gigas, dermis, and green tea are excellent in the platelet aggregation inhibitory effect and can be used for the treatment of arteriosclerosis, hypertension, angina pectoris, myocardial infarction, ischemic heart disease, heart failure, complications following cerebral angioplasty, , And stroke, and the like can be effectively used as a composition capable of effectively preventing or suppressing vascular diseases.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flowchart showing a method for preparing herbal medicine extracts of red ginseng, Angelica gigas, dermis and green tea disclosed in Example 1 of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of the invention to those skilled in the art.

< Example  1. Preparation of red ginseng, Angelica gigas, dermis and green tea extract>

Red ginseng, Angelica japonica, Dermis, and Green tea were mixed under the conditions shown in Table 1, and 12 g of a 30% aqueous ethanol solution was added to 1200 g of the mixture. The mixture was extracted twice at 90 to 95 ° C for 4 hours, To obtain a mixed extract of red ginseng, Angelica gigas, dermis and green tea.

Condition Red ginseng (g) Angelica (g) Dermis (g) Green tea (g) Mixing ratio Example 1-1 400 400 200 200 2: 2: 1: 1 Examples 1-2 600 200 200 200 3: 1: 1: 1 Example 1-3 600 300 150 150 4: 2: 1: 1 Examples 1-4 800 133 133 134 6: 1: 1: 1 Examples 1-5 480 240 240 240 2: 1: 1: 1 Examples 1-6 720 240 120 120 6: 2: 1: 1

< Example  2. Preparation of red ginseng, angelica, dermis and green tea extract ②>

2-1. Using 100% Ethanol

A mixture of each of the extracts of red ginseng, Angelica gigas, dermis, and green tea was prepared in the same manner as in Example 1-4, but the extract was prepared using 100% ethanol.

2-2. Water use

As in Example 1-4, a mixture of the respective extracts of red ginseng, Angelica gigas, dermis, and green tea was prepared, and water was used to prepare an extract.

< Comparative Example  1. Comparison of red ginseng, angelica, dermis and green tea extracts ①>

The extracts of red ginseng, Angelica gigas, dermis and green tea mixture were prepared in the same manner as in Example 1, except that the respective extracts were mixed under the conditions shown in Table 2 below.

Condition Red ginseng (g) Angelica (g) Dermis (g) Green tea (g) Mixing ratio Comparative Example 1-1 1200 - - - 1: 0: 0: 0 Comparative Example 1-2 - 1200 - - 0: 1: 0: 0 Comparative Example 1-3 - - 1200 - 0: 0: 1: 0 Comparative Example 1-4 - - - 1200 0: 0: 0: 1 Comparative Example 1-5 600 600 - - 1: 1: 0: 0 Comparative Example 1-6 600 - 600 - 1: 0: 1: 0 Comparative Example 1-7 600 - - 600 1: 0: 0: 1 Comparative Example 1-8 - 600 600 - 0: 1: 1: 0 Comparative Example 1-9 - 600 - 600 0: 1: 0: 1 Comparative Example 1-10 - - 600 600 0: 0: 1: 1 Comparative Example 1-11 400 400 400 - 1: 1: 1: 0 Comparative Example 1-12 400 400 - 400 1: 1: 0: 1 Comparative Example 1-13 400 - 400 400 1: 0: 1: 1 Comparative Example 1-14 - 400 400 400 0: 1: 1: 1 Comparative Example 1-15 924 92 92 92 10: 1: 1: 1 Comparative Example 1-16 92 924 92 92 1: 10: 1: 1 Comparative Example 1-17 92 92 924 92 1: 1: 10: 1 Comparative Example 1-18 92 92 92 924 1: 1: 1: 10 Comparative Example 1-19 686 342 86 86 8: 4: 1: 1 Comparative Example 1-20 134 800 133 133 1: 6: 1: 1 Comparative Example 1-21 100 100 500 500 1: 1: 5: 5

< Experimental Example  1. Identification of the inhibitory effect of the extract on platelet aggregation>

1-1. Principle of experiment

Plasma was extracted from the rabbits and washed platelets (WP, Wash. et al. (Non-Patent Document 19). Agonist (inducer) was collagen (Chronolog Co. USA # 385) and platelet aggregation inhibition was measured by Born G.V. was measured by turbidity method using a platelet aggregometer (ChronoLog Co. USA) according to the method described in Non-Patent Document 20.

1-2. Platelet rich plasma ( platelet rich plasma )

45 ml of blood was collected from the ear artery of rabbits using a tube containing 5% of 3.8% (w / v) sodium citrate as an anticoagulant (sodium citrate: blood = 1: 9, v / v). Platelet rich plasma (PRP) was obtained from the supernatant by centrifugation (Vision Co., Korea) for 10 minutes at 120 × g at room temperature.

1-3. Rabbit wash platelets ( rabbit washed platelet )

The platelet-rich plasma was centrifuged at 300 × g for 10 min and the precipitate was washed twice with 2 mM EDTA (ethylene diamine tetraacetic acid) in a washing buffer (129 mM NaCl, 0.8 mM KH ₂ PO ₄ , 8.9 mM NaHCO ₃ , 2.8 washed with _{mM KCl, 0.8mM MgCl 2, 5.6mM} glucose, 10mM HEPES, pH 7.4). Thereafter, the washed precipitate was washed with 0.35% bovine serum albumin-containing Tyrode's buffer (129 mM NaCl, 0.8 mM KH ₂ PO ₄ , 8.9 mM NaHCO ₃ , 2.8 mM KCl, 0.8 mM MgCl ₂ , 5.6 mM glucose, 10 mM HEPES, pH 7.4). Platelet counts were adjusted to 4 x 10 ⁸ cells / ml using a Coulter Counter (Coulter Electronics, Hialeah, FL, USA).

1-4. Platelet aggregation measurement

247.5 토 of rabbit-washed platelets were added, and 1 mM CaCl ₂ was added. After incubation at 37 캜 with stirring at 1,000 rpm, the herbal medicine extracts of Examples and Comparative Examples were dissolved in DMSO (dimethyl sulfoxide, and DMSO to a final concentration of 0.1% or less), and after 3 minutes elapsed, agonist collagen was administered to induce platelet aggregation. Platelet activation inhibition was determined by using collagen-induced aggregation (%) as a control group (A) and pretreatment of each herb extract to induce aggregation (%) as a sample aggregation The percent inhibition was expressed as inhibition (%).

A: control aggregation (%)

B: sample aggregation (%)

1-5. Platelet aggregation measurement result

The results of measurement of the platelet aggregation of the respective samples are shown in Table 3 below.

Condition Platelet aggregation inhibition rate (%) Example 1-1 95.4 ± 2.7 Examples 1-2 92.5 ± 1.5 Example 1-3 85.6 ± 2.6 Examples 1-4 96.7 ± 3.4 Examples 1-5 84.8 ± 1.5 Examples 1-6 93.9 ± 2.5 Example 2-1 92.6 ± 3.2 Example 2-2 79.5 ± 0.9 Comparative Example 1-1 27.0 ± 1.6 Comparative Example 1-2 44.6 ± 2.3 Comparative Example 1-3 12.6 ± 2.4 Comparative Example 1-4 10.5 ± 1.5 Comparative Example 1-5 43.3 ± 0.6 Comparative Example 1-6 33.4 ± 0.7 Comparative Example 1-7 34.5 ± 0.8 Comparative Example 1-8 46.6 ± 2.5 Comparative Example 1-9 47.7 ± 1.6 Comparative Example 1-10 12.4 ± 2.7 Comparative Example 1-11 43.5 ± 3.5 Comparative Example 1-12 47.7 ± 1.4 Comparative Example 1-13 31.4 ± 2.5 Comparative Example 1-14 42.3 ± 1.3 Comparative Example 1-15 33.4 ± 1.4 Comparative Example 1-16 42.7 ± 1.7 Comparative Example 1-17 47.4 ± 2.8 Comparative Example 1-18 42.5 ± 1.5 Comparative Example 1-19 44.7 ± 1.6 Comparative Example 1-20 41.8 ± 0.4 Comparative Example 1-21 19.2 ± 0.7

< Experimental Example  2: Toxicity test>

2-1. Acute toxicity

This experiment was conducted to investigate the toxicity of the herbal extracts of red ginseng, angelica gingiva, dermis, and green tea of the present invention to an animal body in an acute (within 24 hours) when an excessive amount of herbal extracts were taken in a short period of time and to determine the mortality rate. Twenty ICR mice were injected into each of the control and experimental groups. In the control group, only the PEG-400 / tween-80 / ethanol (8/1/1, v / v / v) was administered and the herbal extracts of red ginseng, Were dissolved in 400 / tween-80 / ethanol (8/1/1, v / v / v) and orally administered at a concentration of 2 g / kg / day. After 24 hours of administration, the mice were found to survive in the control group and in the experimental group administered with herbal extracts of red ginseng, Angelica gigas, dermis and green tea at a concentration of 2 g / kg / day.

2-2. Experimental group  And control organ organs and tissue toxicity experiments

For the long-term toxicity test, the herbal extracts of red ginseng, Angelica gigas, dandruff and green tea of Example 1-4 were administered to C57BL / 6J mice (10 per group) for 8 weeks at each concentration (final concentration 2g / Respectively.

In order to investigate the effects on the organs (tissues) of animals, the experimental group in which the herbal extracts of red ginseng, Angelica gigas, dermis and green tea of Example 1-4 were administered and PEG-400 / tween-80 / ethanol (8/1/1 (Vital Scientific NV, Netherland) were used to determine the concentration of glutamate-pyruvate transferase (GPT) and blood urea nitrogen (BUN) in the blood after 8 weeks from the control group, Were measured using the instrument. As a result, GPT, which is known to be related to hepatotoxicity, and BUN, which is known to be related to renal toxicity, showed no significant difference compared to the control group. In addition, liver and kidney were sampled from each animal, and histological observation was performed with an optical microscope through a conventional tissue section production process. No abnormalities were observed in all tissues.

< Formulation example  1: Pharmaceutical preparations>

1-1. Manufacture of tablets

200 g of herbal extracts of red ginseng, Angelica gigas, dandelion and green tea of Example 1-4 were mixed with 175.9 g of lactose, 180 g of potato starch and 32 g of colloidal silicic acid. To this mixture was added a 10% gelatin solution, which was pulverized and passed through a 14-mesh sieve. This was dried, and a mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into tablets.

1-2. Injection preparation

1 g of herbal medicine extracts of red ginseng, Angelica gigas, dermis and green tea of Example 1-4, 0.6 g of sodium chloride and 0.1 g of ascorbic acid were dissolved in distilled water to make 100 ml. This solution was placed in a bottle and sterilized by heating at 20 DEG C for 30 minutes.

< Formulation example  2: Food manufacturing>

2-1. Manufacture of cooking seasonings

The herbal extracts of red ginseng, Angelica gigas, dermis, and green tea of Example 1-4 of the present invention were added to the cooking seasoning at 1% by weight to prepare health-enhancing cooking seasonings.

2-2. Manufacture of flour food products

Bread, cake, cookies, crackers and noodles were prepared by adding 0.1% by weight of herbal medicine extract of red ginseng, Angelica gigas, dandelion and green tea of Example 1-4 of the present invention to wheat flour, .

2-3. soup  And juicy ( gravies )

Health enhancing soup and juice were prepared by adding herbal extracts of red ginseng, Angelica gigas, dermis and green tea of Example 1-4 of the present invention to soup and juice at 0.1 wt%.

2-4. dairy product( dairy products )

The herbal extracts of red ginseng, Angelica gigas, dermis and green tea of Example 1-4 of the present invention were added to milk in an amount of 0.1% by weight and various dairy products such as butter and ice cream were prepared using the milk.

2-5. Vegetable juice  Produce

0.5 g of herbal medicine extracts of red ginseng, Angelica gigas, dermis and green tea of Example 1-4 of the present invention were added to 1,000 ml of tomato juice or carrot juice to prepare vegetable juice for health promotion.

2-6. Fruit juice  Produce

Health enhancing fruit juice was prepared by adding 0.1 g of herbal medicine extract of red ginseng, Angelica gigas, dermis and green tea of Example 1-4 of the present invention to 1,000 ml of apple juice or grape juice.

Claims (9)

A composition for preventing or treating a vascular disease selected from arteriosclerosis, angina pectoris, myocardial infarction, ischemic heart disease, cerebral infarction, and stroke, which comprises a herbal extract of red ginseng, Angelica gigas, dermis and green tea. The method according to claim 1,
The herbal medicine extract of red ginseng, Angelica giganteus, dermis, and green tea is an extract of a herbal medicine mixture wherein red ginseng, Angelica gigas, dermis and green tea are mixed at a weight ratio of 2: 6: 1 to 2: 1: 1. Angina pectoris, myocardial infarction, ischemic heart disease, cerebral infarction, and stroke. 3. The method according to claim 1 or 2,
Wherein the herbal medicine extracts of red ginseng, Angelica gigas, dermis, and green tea are extracted with an extraction solvent selected from among water, an aqueous alcohol solution, and alcohol, which is selected from the group consisting of arteriosclerosis, angina pectoris, myocardial infarction, ischemic heart disease, Or a pharmaceutically acceptable salt thereof. delete A health functional food for preventing or ameliorating a vascular disease selected from arteriosclerosis, angina pectoris, myocardial infarction, ischemic heart disease, cerebral infarction, and stroke, characterized by containing a herbal extract of red ginseng, Angelica gigas, dermis and green tea. 6. The method of claim 5,
The herbal medicine extract of red ginseng, Angelica giganteus, dermis, and green tea is an extract of a herbal medicine mixture wherein red ginseng, Angelica gigas, dermis and green tea are mixed at a weight ratio of 2: 6: 1 to 2: 1: 1. A health functional food for preventing or ameliorating a vascular disease selected from angina pectoris, myocardial infarction, ischemic heart disease, cerebral infarction, and stroke. The method according to claim 5 or 6,
Wherein the herbal medicine extracts of red ginseng, Angelica gigas, dermis, and green tea are extracted with an extraction solvent selected from among water, an aqueous alcohol solution, and alcohol, which is selected from the group consisting of arteriosclerosis, angina pectoris, myocardial infarction, ischemic heart disease, &Lt; / RTI &gt; or a pharmaceutically acceptable salt thereof. delete (1 step) adding an extracting solvent of 5 to 15 times the weight of the herbal mixture to the herbal mixture mixed with the weight ratio of 2: 6: 1 to 2: 1: 1 of red ginseng, Angelica jezoensis, dermis and green tea;
(Step 2) Extracting the mixture of the crude drug mixture and the extraction solvent at a temperature of 40 to 120 ° C for 2 to 24 hours and filtering to obtain a filtrate; And
(Step 3) A step of drying and pulverizing the filtrate. The method for producing a herbal extract of red ginseng, Angelica gigas, dermis and green tea.

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