KR102150162B1 - Liquid composition for stick-type container comprising active mountain-cultivated ginseng extract having increased ginsenosides of human body absorption type, and preparation method thereof - Google Patents

Abstract

In the present invention, including active wild ginseng extract, red ginseng concentrate, bellflower concentrate, black garlic concentrate, and honey, the human body absorbable ginsenosides Rd2, F2, Rg3 and compound K content are significantly enhanced, excellent antioxidant activity, anti-diabetes and anti-obesity Disclosed is a liquid composition for a stick-shaped container that has activity and is convenient to carry and eat.

Description

Liquid composition for stick-type container comprising active mountain-cultivated ginseng extract having increased ginsenosides of human body absorption type, and preparation method thereof}

The present invention relates to a liquid composition for a stick-type container comprising an active wild ginseng extract with enhanced human absorption-type ginsenoside and a method for manufacturing the same, and more particularly, to a human-absorbable ginsenoside Rd2, including an active wild ginseng extract, A liquid composition for a stick container with improved content of F2, Rg3 and compound K, and excellent antioxidant activity, anti-diabetic activity and anti-obesity activity due to enhanced total phenolics and total flavonoids content, and its It relates to a manufacturing method.

Ginseng is classified into ginseng (人蔘), wild ginseng (山蔘), or wild ginseng (山羊蔘) according to the difference between artificial and natural growth according to the cultivation environment and the difference in morphology. In particular, wild ginseng belongs to the family Ogapidae and refers to the artificially grown wild ginseng seeds or yusams grown in the mountains by Panax ginseng , a perennial plant, in the wild under the forest in the mountains, and these are called wild ginseng. In addition, wild ginseng grown in the wild from ancient times has been hit as the best wild ginseng and is also called camphor ginseng. Most of ginseng has 3-7 outcrops, but wild ginseng is more than that depending on age. The roots of ginseng are thick and short, but wild ginseng is long and thin. Wild ginseng is known to be more effective than cultivated ginseng in terms of various efficacy, but there is not much scientific evidence to support it than ginseng.

Saponin is a generic term for tritelfen and steroid-based glycoside compounds that are widely distributed in the plant world. The meaning of saponin is derived from the Latin word sapo (soap), and it is a natural surfactant that forms bubbles when shaken with water. Sanyangsam, which contains ginsenoside, the saponin of ginseng, is highly evaluated for its efficacy after natural wild ginseng in herbal medicine prescriptions. In addition, it has been reported that the content of ginsenosides is higher than that of cultivated ginseng, and it is known to be superior to cultivated ginseng. However, known processed products related to wild ginseng do not contain ginsenosides or ginsenosides that are absorbed by the human body. Conventionally, some technologies for converting ginsenosides contained in ginseng into human absorption type using enzymes have been developed (Registration Patent No. 10-1751305), but these technologies are expensive or increase rate of human absorption type ginsenosides. Because of this low problem, there is still a demand for the development of a hemp product with improved human absorption-type ginsenosides.

Red ginseng is known to have anticancer, blood pressure lowering, cranial nerve cell protection, antithrombosis, and antioxidant effects, but only a small amount of useful components absorbed by the human body such as ginsenosides Rd2, F2, Rg3 and compound K It is produced and has a limitation in its action, so the use of combinations with other ingredients is being studied to enhance this.

Bellflower ( Platycodon grandiflorum ) is a perennial plant that grows wild in Korea, northeastern China, and Japan, and grows in the grasslands of sunny mountains. The roots of bellflower have been used for edible vegetables or medicinal purposes, and the roots are washed and dried with water or peeled and dried and dried. Roots contain 2% triterpenoid saponin and 0.03% sterol. Other saccharides include inulin and platicodinin. The main effect of bellflower in oriental medicine is known to be good for symptoms such as coughing, phlegm, and other lung disorders and close sore throat, dysentery, difficulty urinating, diarrhea, and after-jung (後重).

Black garlic is garlic ( Allium sativum for. Pekinense MAKINO) is fermented and aged, and water-soluble sulfur-amino acids called S-allylcysteine (SAC) and S-allyl-mercapto-cysteine, which are not present in raw garlic, are produced to prevent cancer, improve arteriosclerosis, and prevent heart disease. It is known that the effect of various pharmacological actions is higher than that of raw garlic.

Accordingly, as a result of continuing research to meet the requirements of the prior art, the inventors of the present invention resulted in a liquid containing active wild ginseng extract, red ginseng concentrate, bellflower concentrate, black garlic concentrate, and honey prepared by subjecting wild ginseng to high-temperature aging under specific conditions. The composition has markedly improved human absorption-type ginsenosides Rd2, F2, Rg3 and compound K content, has excellent antioxidant activity, anti-diabetic and anti-obesity activity, and is suitable for use in stick-type containers that are convenient to carry and ingest. Confirmed and completed the present invention.

Therefore, the object of the present invention is to significantly improve the content of ginsenosides Rd2, F2, Rg3 and compound K absorbed by the human body, including active wild ginseng extract, red ginseng concentrate, bellflower concentrate, black garlic concentrate, and honey, excellent antioxidant activity, antidiabetic And it has anti-obesity activity, to provide a liquid composition for a stick-type container that is convenient to carry and ingest.

Another object of the present invention is a liquid for a stick-type container that is easy to carry and ingest, which significantly enhances the human body absorption type ginsenosides Rd2, F2, Rg3 and compound K content, has excellent antioxidant activity, anti-diabetic and anti-obesity activity. It is to provide a method for preparing the composition.

Another object of the present invention is to provide a food having antioxidant activity, diabetes and obesity improvement and prevention functions comprising the liquid composition.

In order to achieve the above object, the present invention provides active wild ginseng extract 15-25 vol%, red ginseng concentrate 15-25 vol%, bellflower concentrate 15-25 vol%, black garlic concentrate 4-6 vol%, honey 6-12 vol%, It is to provide a liquid composition for a stick-type container containing 15 to 20% by volume of liquid dextrin, and the rest of water, and enhanced human body absorption ginsenosides Rd2, F2, Rg3 and compound K.

<Active wild ginseng extract>

In the present invention, wild ginseng refers to ginseng produced without installing artificial facilities such as a light shield in the mountain area defined in Article 2 No. 1 of the 「Mountain Management Act」, and is preferably grown between 3 and 5 years old. .

In the present invention, "active wild ginseng" refers to processed wild ginseng so that the contents of human absorbable ginsenosides Rd2, F2, Rg3 and compound K are increased.

In the present invention,'steaming process' means steaming with water vapor at 100°C for 30 to 60 minutes.

If the steaming time is less than 30 minutes, sufficient steaming may not proceed and subsequent steps such as ripening may not be smooth, and contamination of various germs may occur.If steaming for more than 60 minutes, the physiologically active ingredients of wild ginseng will be reduced by long heat treatment. It can be destroyed.

In the present invention,'high temperature aging' means maintaining at 70 to 90°C for 2 to 4 days.

If the aging temperature is less than 70℃ or the aging period is less than 2 days, the aging does not proceed smoothly. If the aging temperature is more than 90℃ or the aging period is more than 4 days, the produced physiologically active substance may be decomposed and the content may be reduced. .

In the present invention, the active wild ginseng can be prepared by repeating the above steaming and high temperature aging three times.

In the present invention, the'active wild ginseng extract' is powdered to 100 mesh or less after drying the active wild ginseng prepared as described above at 50 to 55°C for 2 to 3 days, and then 10 to 20 times (v/w) in the active wild ginseng powder. ) It can be prepared by adding water and extracting at 80-100° C. for 5-10 hours, then filtering and concentrating so that the solid content is 10% or more.

The active wild ginseng extract according to the present invention contains ginsenosides Rd2, F2, Rg3 and compound K of 2.77 mg/ml or more, 1.27 mg/ml or more, 1.03 mg/ml or more, and 2.13 mg/ml or more, compared to before processing. , Respectively, about 1.5 times or more, 4 times or more, 2.8 times or more and 4.2 times or more, respectively (Table 1).

In the liquid composition according to the present invention, the active wild ginseng extract is contained in an amount of 15 to 25% by volume, and when the active wild ginseng extract is added less than 15% by volume, the physiological activity is insufficient due to the small amount of human absorbable ginsenoside, and exceeds 25% by volume. When added, the cost of the product increases due to the high cost of raw materials for wild ginseng, and the high ginsenoside content may cause toxicity to the human body.

<Red ginseng, bellflower, Black garlic Concentrate>

In the present invention, the red ginseng concentrate is used having a solid content of 10% or more.

Red ginseng concentrate is prepared by adding 10-20 times (v/w) to red ginseng root and/or red rice ginseng powder, extracting for 5-10 hours at 80-100°C, filtering, and concentrating so that the solid content is 10% or more, or commercially easy You can also get it.

Preferably, the red ginseng concentrate is used in which the sum of ginsenosides Rg1, Rg2 and Rg3 is 4.5 mg/g or more.

In the liquid composition according to the present invention, the red ginseng concentrate is contained in an amount of 15 to 25% by volume, and when less than 15% by volume of red ginseng extract is added, the ginsenoside content is insufficient, and when it is added in excess of 25% by volume, the ginsenoside content is high. Toxicity may be caused.

Bellflower concentrate is prepared by adding 10 to 20 times (v/w) to dry bellflower powder, extracting at 80 to 100°C for 5 to 10 hours, then using the extract as it is or concentrating so that the solid content is 5% or more, or It can also be easily obtained commercially.

In the liquid composition according to the present invention, the bellflower concentrate is contained in 15 to 25% by volume, and when less than 15% by volume of the bellflower extract is added, the physiological activity is insufficient, and when it is added in excess of 25% by volume, the palatability is influenced by the characteristic flavor and taste of bellflower. Can be crazy.

Black garlic concentrate is prepared by adding 10-20 times (v/w) to black garlic prepared by high temperature aging, extracting it at 80-100°C for 5-10 hours, and then using the extract as it is by filtration or concentrating so that the solid content is 5% or more. Or, it can be easily obtained commercially.

In the liquid composition according to the present invention, the black garlic concentrate is contained in 4-6% by volume, and when less than 4% by volume of the black garlic extract is added, the physiological activity is insufficient, and when it is added over 6% by volume, the taste and taste of black garlic affect palatability. Can be crazy.

<Preparation of liquid composition for stick type container>

Active wild ginseng extract 15-25% by volume, red ginseng concentrate 15-25% by volume, bellflower concentrate 15-25% by volume, black garlic concentrate 4-6% by volume, honey 6-12% by volume, liquid dextrin 15-20% by volume and rest water Is mixed and filled into a stick-shaped container.

In the present invention,'honey' is added and mixed in an amount of 6 to 12% by volume. If less than 6% by volume of honey is contained, it is inconvenient to consume as a dilute liquid, and if it is contained in more than 12% by volume, the sweet taste may be strong.

In the present invention,'liquid dextrin' is added and mixed in an amount of 15 to 20% by volume. If the liquid dextine is contained in an amount of less than 15% by volume, it is inconvenient to intake as a dilute liquid.

If necessary, the liquid composition of the present invention may further contain a fruit concentrate, a herbal medicine concentrate, and a sweetener (aspartame, stevia, etc.).

In the present invention,'filling' may be performed by a conventional method known in the art.

In the liquid composition according to the present invention, the contents of ginsenosides Rd2, F2, Rg3 and compound K are 0.08 mg/ml, 0.12 mg/ml, 0.08 mg/ml and 0.50 mg/ml, respectively, compared to the comparative example, respectively, about It is improved more than 2 times, more than 3 times, more than 1.5 times and more than 2.7 times (Table 3).

In addition, the liquid composition according to the present invention has excellent antioxidant activity due to the enhanced content of phenolics and flavonoids, and has excellent alpha-glucosidase inhibitory activity and pancreatic-lipase inhibitory activity, so that antidiabetic activity and antiobesity activity are significantly enhanced. (Fig. 3, Fig. 4, Table 6), it has a concentration (viscosity) of a degree that is easily ingested and filled in a stick-shaped container.

According to another object of the present invention, the present invention

Ⅰ) preparing active wild ginseng by repeating the steaming of wild ginseng at 100°C for 30 to 60 minutes and high-temperature aging for 2 to 4 days at 70 to 90°C for 3 times;

Ii) After drying the activated wild ginseng for 2 to 3 days at 50 to 55°C, powder it to less than 100 mesh, add 10 to 20 times (v/w), extract for 5 to 10 hours at 80 to 100°C, and filter. Preparing an active wild ginseng concentrate by concentrating so that the solid content is 10% or more; And

Iii) 15 to 25 vol% of active wild ginseng extract, 15 to 25 vol% of red ginseng concentrate, 15 to 25 vol% of bellflower concentrate, 4 to 6 vol% of black garlic concentrate, 6 to 12 vol% of honey, 15 to 20 vol% of liquid dextrin, and Stick-type with improved human absorption-type ginsenosides Rd2, F2, Rg3 and Compound K, including the step of mixing the remaining water and filling it into a stick-shaped container, and having excellent antioxidant activity and enhanced antidiabetic and antiobesity activity It provides a method for producing a liquid composition for a container.

Step i): Active wild ginseng Produce

The manufacturing process of the active samyangsam extract is as described above.

Sanyangsam is provided by washing three-year-old wild ginseng with water to remove moisture.

Steaming is performed at 100°C for 30 to 60 minutes, and can be performed using a steamer such as a conventional steamer.

When the steamed wild ginseng is aged at a high temperature, it can be carried out by allowing moisture to evaporate using an open container or using a closed container to prevent moisture from evaporating.

Step ii) Active wild ginseng Extract preparation

The manufacturing process of the active wild ginseng extract is as described above.

Filtration is performed with a 2 to 8 mm filter filter,

Concentration is a thermal concentration method that applies heat or a vacuum concentration method so that the solid content is 10% or more.

Iii) Preparation of liquid composition

Active wild ginseng extract 15-25% by volume, red ginseng concentrate 15-25% by volume, bellflower concentrate 15-25% by volume, black garlic concentrate 4-6% by volume, honey 6-12% by volume, liquid dextrin 15-20% by volume and rest water Mix and fill in a stick-shaped container.

Mixing can be carried out by a conventional method known in the art using a stirring mixer.

Filling may be performed by a conventional method known in the art by using a stand stick filling machine.

If necessary, in the production method of the present invention, in step iii), a fruit concentrate, a herbal medicine concentrate, and/or a sweetener (aspartame, stevia, etc.) may be further mixed.

The liquid composition prepared by the manufacturing method of the present invention has increased contents of ginsenosides Rd2, F2, Rg3 and compound K, and enhanced antioxidant activity, antidiabetic activity and anti-obesity by enhancing the content of phenolics and flavonoids. Have activity

According to another object of the present invention, the present invention provides a food having antioxidant activity, diabetes and obesity improvement and prevention functions comprising a liquid composition prepared by the above manufacturing method.

In the present invention, the food may be processed in the form of a product in the form of a beverage, jelly, capsule, or the like using the liquid composition of the present invention as it is.

The liquid composition for a stick-type container containing the active wild ginseng extract according to the present invention has significantly improved the content of ginsenosides Rd2, F2, Rg3 and compound K, and the content of phenolics and flavonoids is enhanced and excellent antioxidant activity. , As it has alpha-glucosidase inhibitory activity and pancreas-lipase inhibitory activity, anti-diabetic activity and anti-obesity activity are enhanced, so it can be used as a material for functional foods and drugs. Also, it is easy to carry and ingest as it is for a stick-type container.

The manufacturing method according to the present invention makes it possible to produce a liquid composition for a stick-type container with significantly improved contents of ginsenosides Rd2, F2, Rg3 and compound K compared to a conventional method.

Functional food according to the present invention has excellent antioxidant activity, anti-diabetic activity and anti-obesity activity, so as to inhibit adipogenesis, weight control, lower cholesterol, improve hyperlipidemia, alleviate arteriosclerosis, alleviate diabetes, improve blood circulation, and improve immunity. Can be used.

1 shows an example of a manufacturing process of a liquid composition for a stick-type container containing the active wild ginseng extract according to the present invention.
Figure 2 shows a ginsenoside HPLC chromatogram of a liquid composition for a stick-type container containing the active wild ginseng extract according to the present invention. 2A is a ginsenoside HPLC chromatogram of a comparative example, and FIG. 2B is a ginsenoside HPLC chromatogram of an example.
Figure 3 shows the antioxidant activity of the liquid composition for a stick-type container containing the active wild ginseng extract according to the present invention. Figure 3a shows the DPPH radical scavenging activity, Figure 3b shows the ABTS radical scavenging activity, Figure 3c shows the FRAP reducing power.
Figure 4 shows the digestive enzyme inhibitory activity of the liquid composition for a stick-type container containing the active acid mass production extract according to the present invention. Figure 4a shows the alpha-glucosidase inhibitory activity, Figure 3b shows the pancreatic-lipase inhibitory activity.

The present invention will be described in more detail through the following examples. These examples are for illustrative purposes only, and the scope of the present invention should not be limited thereto.

Preparation Example 1: Preparation of active wild ginseng extract

10 kg of wild ginseng grown for more than 3 years in the area of Seosang-gun, Hamyang-gun, Gyeongsangnam-do, was washed three times with running water and completely drained, and then 5 kg was put in a steamer and steamed for 60 minutes at 100°C. The steamed wild ginseng was put on a dry tray and aged at 75°C for 3 days while allowing moisture to evaporate. The same weight of wild ginseng that has been aged for 3 days and dried Moisture (moisture content of about 40%) was added, steamed at 100°C for 60 minutes, and re-aged at 75°C for 3 days. The same weight of wild ginseng that has been aged and dried twice Moisture (moisture content about 40%) was added, steamed at 100°C for 60 minutes, and last aged at 75°C for 3 days to complete active wild ginseng (Fig. 1).

The prepared active wild ginseng was dried at 50-55° C. for 2 days and then pulverized to less than 100 mesh with a grinder to prepare a powder. After adding 500 ml of 10 times purified water to 50 g of the powder, extracting for 8 hours at 100°C, filtering with an 8 mm filtration filter, and concentrating the filtrate by applying conventional heat so that the solid content is 10% or more, Example 1) was prepared.

For comparison, wild ginseng was dried, powdered, extracted, and concentrated in the same manner as above to prepare and use a wild ginseng extract (Comparative Example 1).

<Analysis of Ginsenoside Content>

The active wild ginseng extract of Example 1 prepared above and the wild ginseng extract of Comparative Example 1 were each filtered through a 0.45 μm membrane filter and used as analysis samples.

Ginsenoside analysis was performed by high press liquid chromatography (HPLC) by modifying the method described in the functional food analysis method. TSKgel ODS-100Z was used as the analysis column, and the sample injection amount was 10 μl, the temperature was 30°C, the measurement wavelength was 203 nm, and the flow rate was 1.0 ml/min. As the mobile phase, HPLC water was used for solution A and acetonitrile was used for solution B. . As for the HPLC analysis conditions, when the mobile phase solution is 0 minutes, flow A solution 81%: B solution 19%, 15 minutes, A solution 80%: B solution 20%, 40 minutes A solution 77%: B solution 23%, 42 minutes Solution A 70%: Solution B 30%, Solution A 65% at 75 minutes: Solution B 35%, Solution A 30% at 80 minutes: Solution B 70%, Solution A 10% at 90 minutes: Solution B 90% mobile phase Spilled. The contents of ginsenosides absorbed by the human body of Example 1 (active wild ginseng extract) and Comparative Example 1 (wild ginseng extract) analyzed are shown in Table 1.

Content (mg/ml)* Comparative Example 1 Example 1 Ginsenoside Rd2 1.81 2.77 Ginsenoside F2 0.31 1.27 Ginsenoside Rg3 0.36 1.03 Ginsenoside C.K 0.50 2.13

As shown in Table 1, when looking at the content of ginsenosides absorbed by the human body, the active wild ginseng extract of Example 1 has ginsenoside Rd2 of 2.77 mg/ml, F2 of 1.27 mg/ml, Rg3 of 1.03 mg/ml, and Compound K was measured at 2.13 mg/ml, and it can be seen that, compared to the wild ginseng extract of Comparative Example 1, about 1.5 times, 4 times, 2.8 times, and 4.2 times or more were improved.

Manufacturing example 2: Preparation of liquid composition for stick-type container

A liquid composition was prepared by mixing the active wild ginseng extract prepared in Preparation Example 1 and the wild wild ginseng extract at the mixing ratio shown in Table 2 below.

Mixing ratio (volume%) Comparative Example 2 Example 2 Wild ginseng extract (more than 10% solid content) 20 - Active wild ginseng extract (more than 10% solid content) - 20 Red ginseng concentrate 20 20 Bellflower concentrate 20 20 Black Garlic Concentrate 5 5 honey 10 10 Liquid dextrin 15 15 Purified water 10 10 Sum 100 100

Red ginseng concentrate is manufactured by EFG Co., Ltd. located in Geumsan-gun, Chungnam (the sum of ginsenosides Rg1, Rg2 and Rg3 is 4.5 mg/g or more, solid content is 10% or more, 70% red ginseng root and 30% red ginseng are mixed), bellflower concentrate and Black garlic concentrate was also manufactured by EFG, Inc., and liquid dextrin and honey were purchased on the market.

The above materials were mixed using a stirring mixer, and 12 ml each were filled in a stick packaging paper using a stand stick filling machine.

Test example

Human absorption-type ginsenoside content, phenolics and flavonoids content, antioxidant activity, anti-diabetic activity and anti-obesity activity, and nutritional components of the liquid composition comprising the active wild ginseng extract of Example 2 prepared in Preparation Example 2 Scientific properties and free amino acids were analyzed.

Test example 1. Analysis of human absorbable ginsenoside content

Analysis of the content of human absorbable ginsenosides for each of the liquid composition comprising the active wild ginseng extract of Example 2 prepared in Preparation Example 2 and the liquid composition including the wild ginseng extract of Comparative Example 2 was described in Preparation Example 1. Analysis was performed using HPLC in the same manner as in the method, and the results are shown in FIGS. 2 and 3.

Content (mg/ml) Comparative Example 2 Example 2 Ginsenoside Rd2 0.04 0.08 Ginsenoside F2 0.04 0.12 Ginsenoside Rg3 0.06 0.08 Ginsenoside compound K 0.18 0.50

As shown in FIG. 2, four types of human-absorbable ginsenoside derivatives of Rd2, F2, Rg3, and Compound K were detected in each of the liquid compositions.

As shown in Table 3, the liquid composition containing the active wild ginseng extract of Example 2 was compared to the liquid composition containing the wild ginseng extract (Comparative Example 2), 2 times the amount of ginsenoside Rd2 absorbed by the human body, 3 times the F2, It can be seen that Rg3 is increased by 1.5 times and Compound K is increased by more than 2.7 times.

Test example 2. Gun Phenolics And flavonoids content analysis

The total phenolics and total flavonoids contents of the liquid composition containing the active wild ginseng extract of Example 2 prepared in Preparation Example 2 were measured by the Folin-Denis and Davis methods.

The total phenolics content is determined by the liquid composition. After 100-fold dilution, 0.5 ml of this diluted solution and 0.5 ml of 2N Folin-Ciocalteu phenol reaction reagent were dispensed into a test tube, and 0.5 ml of a 25% Na ₂ CO ₃ solution was additionally added, followed by color development at 30° C. for 1 hour. The absorbance of the blue color developed after 1 hour was measured at 750 nm using a spectrophotometer, and the content was calculated from a standard calibration curve using gallic acid, and is shown in Table 4.

For the total flavonoid content, 1 ml of diethylene glycol was added to and mixed with 1 ml of the composition obtained by diluting the liquid composition 100 times, and 0.01 ml of 1N NaOH solution was added thereto, followed by reaction in a water bath at 37° C. for 1 hour. After 1 hour, the absorbance was measured at 420 nm, and the content was calculated from a standard calibration curve using a routine and shown in Table 4.

Analysis item Example 2 Total phenolics (mg/ml) 3.79 Total flavonoids (mg/ml) 1.83

As shown in Table 4, the total phenolics and total flavonoids contents of the liquid composition of the present invention (Example 2) were high at 3.79mg/ml and 1.83mg/ml, respectively.

Test example 3. Antioxidant activity assay

For the liquid composition (Example 2) containing the active wild ginseng extract according to the present invention was analyzed by measuring DPPH, ABTS and FRAP reducing power, which are indicators of the antioxidant effect.

<DPPH radical scavenging activity>

The liquid composition of Example 2 prepared in Preparation Example 2 was diluted 2 times, 5 times, and 10 times with distilled water, respectively 0.2 ml and 0.8 ml of DPPH solution (1.5×10 ^-4 M) were mixed in the dark for 30 minutes. It was reacted at and absorbance was measured at 517 nm. The negative control of DPPH radical scavenging activity was performed in the same manner using distilled water instead of the sample, and the difference in absorbance was calculated as a percentage (%) by the following equation, and the results are shown in FIG. 3A:

Radical scavenging activity (%) = [1-(absorbance in negative control/absorbance in experimental area)]×100

As shown in FIG. 3A, the liquid composition diluted 2 times showed a high DPPH radical scavenging activity as 88.72%, and in particular, the liquid composition diluted 10 times showed more than 60% DPPH radical scavenging activity.

<ABTS radical scavenging activity>

ABTS radical scavenging activity is a method of measuring antioxidant activity according to the degree of reduction of the blue-colored radicals of 2-azino-bis. This method has the advantage of being able to measure in a short time since the decolorization reaction ends within 1 minute as the ABTS cation is eliminated by the antioxidant activity of the substance and decolorizes to cyan or colorless.

To measure ABTS radical scavenging activity, first, K ₂ S ₂ O ₈ reagent and methanol were mixed in a ratio of 2:1 and reacted for 16 hours in a dark room to generate cation radicals first, and then mixed with methanol to obtain an absorbance value of 0.8±0.2 at 732 nm. The adjusted ABTS reagent was used.

Specifically, 0.1 ml of each of the analysis samples obtained by diluting the liquid composition of Example 2 2 times, 5 times and 10 times with distilled water was added to 0.9 ml of ABTS reagent, reacted for 3 minutes, and the absorbance was immediately measured at 732 nm. The negative control was performed in the same manner using distilled water instead of the sample, and the difference in absorbance was calculated as a percentage (%) by the above equation, and the results are shown in FIG. 3B.

As shown in Fig. 3b, the liquid composition diluted 2 times exhibited a high radical scavenging activity of 90.08%, and in particular, the liquid composition diluted 10 times showed DPPH radical scavenging activity of more than 75%.

<FRAP reducing power>

FRAP (ferric reducing antioxidant power) is a method of measuring the reducing power of compounds, and is a method of measuring the ability of Fe3+ ions to reduce to Fe2+ ions using absorbance values.

Acetate buffer (30 mM, pH 3.6), TPTZ reagent (10 mM in 40 mM HCl), and FeCl ₃ The solution (distilled water containing 20 mM) was mixed in a ratio of 10:1:1 (v/v/v) to prepare a FRAP measurement reagent, and pre-reacted for 15 minutes in a 37°C incubator.

50 µl and 950 µl of FRAP reagent were dispensed, respectively, for analysis samples diluted 2 times, 5 times and 10 times with distilled water, reacted at 37°C for 15 minutes, and absorbance was measured at 595 nm, and the results are shown in FIG.

As shown in Fig. 3c, the FRAP reducing power was high as 1.676 when diluted 2 times, and in particular, the liquid composition diluted 10 times showed a reducing power of 0.987.

Test example 4. Antidiabetic And Anti-obesity Effect analysis

Antidiabetic effect was measured by using two enzyme inhibitory activities using alpha-glucosidase, an indicator, and pancreatic-lipase, an indicator of anti-obesity effect.

<alpha-glucosidase inhibitory activity>

50 µl, respectively, of a liquid composition containing the active wild ginseng extract of Example 2 prepared in Preparation Example 2 and the liquid composition including the wild ginseng extract of Comparative Example 2, 50 µl of alpha-glucosidase enzyme (1.0 U/ml), 200 50 µl of an mM sodium phosphate buffer solution (pH 6.8) was mixed and pre-reacted at 37°C for 10 minutes. Then, 100 µl of p-NPG (5 mM) dissolved in sodium phosphate buffer solution (pH 6.8) was added and reacted again at 37°C for 10 minutes. Na ₂ CO ₃ in this reaction solution After adding 0.75 ml of (100 mM) to stop the final reaction, the absorbance was measured at 420 nm and calculated as a percentage (%) by the above equation using distilled water as a negative control, and is shown in FIG. 4A.

According to FIG. 4A, the liquid composition (Example 2, 26.37%) according to the present invention exhibited an alpha-glucosidase inhibitory activity that was enhanced by 2 times or more compared to Comparative Example 2 (13.7%) when diluted 5 times.

<pancreatic-lipase inhibitory activity>

50 µl, lipase enzyme (1.0 U/ml) 50 µl, and 200 mM, respectively, of the liquid composition containing the active wild ginseng extract of Example 2 prepared in Preparation Example 2 and the liquid composition including the wild ginseng extract of Comparative Example 2 50 µl of sodium phosphate buffer solution (pH 6.8) was mixed and pre-reacted at 37°C for 10 minutes. After the reaction, 100 μl of p-NPB (5 mM) dissolved in sodium phosphate buffer was added and reacted for 10 minutes in the same manner, and then 100 mM Na ₂ CO ₃ The reaction was stopped by adding 0.75 ml, and absorbance was measured at 420 nm. For the negative control, distilled water was taken instead of the sample, and the difference in absorbance between the added and non-added sample solution was calculated as a percentage (%) by the above equation, and the results are shown in FIG. 4B.

As shown in FIG. 4B, when the liquid composition according to the present invention (Example 2) was diluted 5 times, the pancreatic-lipase inhibitory activity was increased by 2 times or more compared to Comparative Example 2.

Test example 5. Physicochemical characterization

The physicochemical properties of the liquid composition containing the active wild ginseng extract of Example 2 prepared in Preparation Example 2 and the liquid composition including the wild ginseng extract of Comparative Example 2 were measured for pH, acidity, soluble solids, reducing sugar and crude protein content.

The pH was measured with a pH meter (MP 200, UK), and the acidity was expressed in% by converting the consumption amount to the amount of lactic acid when neutralized to pH 8.2±0.2 using 0.1 N NaOH. It was measured using. For reducing sugar, 1 ml of DNS reagent was added to 0.1 ml of each composition appropriately diluted according to the DNS method, followed by color development at 100°C for 20 minutes, then rapidly cooled, and absorbance was measured at 570 nm using a spectrophotometer, and compared with a calibration curve.

The water-soluble protein content was measured through the biuret method. To 1.5 g of CuSO ₄ 5H ₂ O (copper sulfate pentahydrate), 6.0 g of KNaC ₄ H ₄ O ₆ 4H ₂ O (sodium potassium stannate) was dissolved in 500 ml of distilled water, and 300 ml of 10% NaOH was added, and the final volume was 1,000. By diagnosing to be ml, a biuret reagent was prepared. 1 g of each liquid composition sample was taken into a test tube, and 4 ml of a biuret reagent was added and mixed thereto, followed by reaction at 37°C for 20 minutes. At this time, the negative control was carried out by taking distilled water, and after 20 minutes reaction, centrifugation for 3 minutes, the supernatant was measured for absorbance at 540 nm, and the water-soluble protein was quantified according to the calculation formula calculated by the protein standard curve (bovine serum albumin). Indicated.

Analysis item Comparative Example 2 Example 2 pH 3.84 3.77 Acidity (%, based on lactic acid) 0.54 0.57 Soluble solids (%) 4.8 4.9 Reducing sugar (mg/ml) 397.68 395.60 Water soluble protein (mg/ml) 151.36 141.21

As shown in Table 5, pH, acidity, soluble solids and reducing sugar contents were similar, and the crude protein content was slightly higher in Comparative Example 2 (151.36 mg/ml) than in Example 2 (141.21 mg/ml).

Test example 6. Analysis of free amino acid content

Free amino acid content analysis was performed using an automatic amino acid analyzer (indicate the device name).

1 ml of the liquid composition including the active wild ginseng extract of Example 2 prepared in Preparation Example 2 and the liquid composition including the wild ginseng extract of Comparative Example 2 and 4 ml of distilled water were mixed and hydrolyzed at 60° C. for 1 hour. 1 ml of 10% 5-sulfosacylic acid dihyarate solution was added and mixed, and then allowed to stand at 4℃ for 2 hours to coagulate the protein, followed by centrifugation, and only the supernatant was filtered through a 0.45 µm membrane filter. It was concentrated under reduced pressure in a water bath. The concentrate was completely dissolved in 2 ml of lithium-citrate (pH 2.2) buffer, filtered again through a 0.45 μm membrane filter, and analyzed for content by an automatic amino acid analyzer, and the results are shown in Table 6.

content (mg/100 ml) Comparative Example 2 Example 2 Common amino acids Aspartic acid 205.04 221.13 Serine 74.52 78.46 Aspartic acid-NH ₂ 85.37 116.31 Glutamic acid 110.96 156.09 Aminoadipic acid 14.87 20.70 Glycine 20.07 24.89 Alanine 161.98 175.11 α-aminobutyric acid 131.69 169.45 Cystine nd 91.63 β-alanine 40.95 55.05 β-aminoisobutyric acid 56.04 68.98 γ-aminobutyric acid 56.33 27.73 Aminoethanol 12.45 15.21 Ornithine 10.70 17.36 3-Methylhistidine 1.43 1.96 Carnosine 15.53 24.02 Arginine 100.03 139.73 sub Total 1168.19 1468.68 Essential amino acids Threonine 48.37 54.90 Methionine 31.72 42.14 Isoleucine 102.59 143.12 Leucine 39.24 46.96 Phenylalanine 84.02 102.47 Lysine 5.44 7.40 Histidine 3.99 5.36 sub Total 461.86 529.44 sum 1630.05 1998.12

From the results of Table 6, most of the general amino acids and essential amino acids were found to be higher in Example 2 than in Comparative Example 2, and the total content of free amino acids in the liquid composition of Example 2 was 1998.12 mg/100 ml.

Claims (5)

Active wild ginseng extract 15-25 vol%, red ginseng concentrate 15-25 vol%, bellflower concentrate 15-25 vol%, black garlic concentrate 4-6 vol%, honey 6-12 vol%, liquid dextrin 15-20 vol%, and the rest Contains water,
The active wild ginseng is prepared by steaming wild wild ginseng at 100°C for 30 to 60 minutes and aging the steamed wild wild ginseng at 70 to 90°C for 2 to 4 days 3 times,
The active wild ginseng extract is powdered to 100 mesh or less after drying the active wild ginseng at 50-55°C for 2 to 3 days, added 10-20 times (v/w) and extracted for 5-10 hours at 80-100°C. And then filtered and concentrated to a solid content of 10%.
It is a liquid composition for a stick-type container with enhanced human body absorption ginsenosides Rd2, F2, Rg3 and compound K,
The active wild ginseng extract is a liquid composition containing ginsenosides Rd2, F2, Rg3 and compound K in an amount of 2.77 mg/ml, 1.27 mg/ml, 1.03 mg/ml and 2.13 mg/ml, respectively.
delete delete delete A method for preparing a liquid composition for a stick-type container with improved human body absorption type ginsenosides Rd2, F2, Rg3 and compound K according to claim 1, wherein the method comprises:
Ⅰ) preparing active wild ginseng by repeating the steamed wild ginseng at 100°C for 30-60 minutes and the steamed wild wild ginseng at 70∼90°C for 2 to 4 days high temperature aging 3 times;
Ii) After drying the activated wild ginseng for 2 to 3 days at 50 to 55°C, powder it to less than 100 mesh, add 10 to 20 times (v/w), extract for 5 to 10 hours at 80 to 100°C, and filter. Concentrating so that the solid content is 10% to prepare an active wild ginseng concentrate; And
Iii) 15 to 25 vol% of active wild ginseng extract, 15 to 25 vol% of red ginseng concentrate, 15 to 25 vol% of bellflower concentrate, 4 to 6 vol% of black garlic concentrate, 6 to 12 vol% of honey, 15 to 20 vol% of liquid dextrin, and Including the step of mixing the remaining water and filling the stick-shaped container,
The active wild ginseng extract contains ginsenosides Rd2, F2, Rg3 and Compound K in amounts of 2.77 mg/ml, 1.27 mg/ml, 1.03 mg/ml and 2.13 mg/ml, respectively.

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