KR101774769B1 - Composition for medicinal herbal tea and manufacturing method of composition for medicinal herbal by supercritical fluid extraction and manufacturing method of carbonated beverage and health beverage thereof - Google Patents

Abstract

The present invention relates to a composition for a herbal composition and a method for preparing a herbal tea extract composition by a CO 2 extraction method, and a method for preparing a carbonated beverage and a health drink using the same. More particularly, In order to contain the herbal medicinal herb extract and the beverage material obtained by extracting and concentrating the herbal medicine raw material made of potatoes with the use of the N2 or CO2 carrier gas, it is preferable to add 10-20 wt% of silkworm, 5-10 wt% of Puerariae, 5 to 10% by weight of liquorice, 5 to 10% by weight of licorice, 20 to 50% by weight of nutrients, and 5 to 10% by weight of jujubes.
5 to 10 wt% of licorice, 5 to 10 wt% of licorice, 20 to 50 wt% of nutrient, 5 to 10 wt% of jujubate, 10 to 20 wt% of silkworm, 5 to 10 wt% By mass of the herbicidal herbicide pulverized by the method of the present invention is extracted and concentrated by a supercritical extraction method using CO 2 or N 2 carrier gas.

Description

TECHNICAL FIELD The present invention relates to a method of preparing a herbal tea extract composition by a supercritical extraction method and a method of manufacturing a carbonated beverage and a health drink by using the herbal composition and the supercritical extraction method. health beverage thereof}

The present invention relates to a method for preparing a herbal tea extract composition using a herbal composition and a supercritical extraction method, and a method for preparing a carbonated beverage and a health drink using the same. More particularly, the present invention relates to a method for preparing a herbal tea, A herbal medicine composition comprising a herbal medicine extract and a beverage material obtained by extracting and concentrating a mixed herbal medicinal raw material with a CO2 or N2 carrier gas and a method of preparing a herbal tea extract composition by a supercritical extraction method, And a method for manufacturing health beverages.

Diabetes mellitus is a chronic non-communicable disease. Insulin secretion from pancreatic β-cells is insufficient or its function is not exerted. Therefore, glucose is not used as an energy source and remains in high concentration in the blood and excreted in the urine.

Diabetes mellitus is divided into two major categories: insulin dependent diabetes mellitus (IDDM), non-insulin dependent diabetes mellitus (NIDDM) and type 2 diabetes mellitus .

Type 1 diabetes is a condition in which the function of the pancreatic β cells is impaired due to genetic causes, viral infections, and insulin is rarely secreted. It occurs mainly in the 10th to 20th generations, and is also referred to as pediatric diabetes.

Type 2 diabetes is caused by family history, obesity, stress, eating disorders, or drugs that antagonize insulin, and is well known in the forties and later, and is also called adult diabetes. Insulin secretion in the pancreas is sufficient, but insulin resistance and glucose utilization are different from normal people, so even though it is hyperinsulinemic, blood sugar is not normalized.

In Korea, more than 90% of diabetic patients are type 2 diabetic.

The symptoms of diabetes are various but typical examples are polyuria, multivitamins, multifetals. Due to high blood sugar levels, glucose and excessive water are excreted into the urine by osmotic pressure, resulting in polyuria Therefore, the symptoms of dehydration in the next thrilling feelings, and also feel hunger, eat a lot of food. In the case of diabetic patients, since glucose is not used as an energy source, it consumes already stored proteins and fats as an energy source, which causes weight loss due to the vicious circle of these phenomena. However, these symptoms are only acute symptoms that appear early in the onset of diabetes.

Diabetes mellitus is classified as Oriental medicine, and it is divided into three categories: upper, lower, and lower. The causes are lung, fever, And renal (renal) fever.

The appeal is also simply called 갈 갈 ((渴 渴), and the fire is moved to the lungs, the fire is frowned, the tongue is red, and the lips are dry and uncomfortable. As a result, ). Small and medium size is the main symptom of diarrhea and diarrhea. The vicious cycle of diarrhea and diarrhea causes weight loss and constipation. Calcination is also called renal excretion, and cigarette is less than appeal, urine is high, and the veins are weak.

Thirsty appellation is better, but it is difficult to treat it if you go to small, calcareous. When thirst is less and weight loss is rapid, calcination has progressed.

Diabetes can be cured if detected early, and early treatment can prevent complications. Early detection of diabetes is to find a state of glucose intolerance. Diabetes mellitus is an intermediate stage between normal and diabetic patients, and is the state before the onset of diabetes. That is, it means that the function of pancreatic beta cells, which regulate blood sugar and secrete insulin, is decreased or insulin resistance is deteriorated and the ability to transport glucose to cells is decreased.

If chronic diabetes mellitus is delayed, chronic complications can lead to chronic vascular disease. Diabetic retinopathy (visual disturbance, blindness, retinal hemorrhage), diabetic nephropathy, diabetic peripheral neuropathy, Sensory function is deteriorated.

The currently used diabetes medicines are broadly divided into oral hypoglycemic agents and insulin injections. In general, insulin-dependent diabetes mellitus or gestational diabetes mellitus patients who do not have insulin secretion in the body, insulin-dependent diabetes mellitus patients whose insulin-dependent diabetes mellitus does not have adequate blood glucose control due to oral hypoglycemic agents, insulin injections, It is widely accepted that patients with non-insulin dependent diabetes mellitus who do not have adequate blood glucose control are given oral hypoglycemic drugs.

Commonly used oral hypoglycemic agents include sulfonylureas drugs, biguanides drugs, and thiazolidinedione drugs.

Sulfonylurea drugs include glipizide, glyclazide, glycidone, glibenclamide, and chlorpropamide, which act to promote insulin secretion in the pancreas. Therefore, they have problems that can not be used for insulin-dependent diabetes mellitus patients who have no insulin secretion in the pancreas, insulin-dependent diabetes mellitus patients whose insulin secretory ability is relatively decreased in the pancreas,

In addition, these drugs cause hypoglycemia when administered in an overdosage dose or fasting, and side effects such as skin rash, jaundice, anorexia, nausea (diarrhea) and diarrhea also appear. In particular, long-half-life drugs (12 to 36 hours), such as chlorpropamide, accumulate in the body and are very likely to cause hypoglycemia. In addition, since most sulfonylurea drugs are metabolized in the liver and excreted in the kidney, they should be used with caution in patients with hepatic and renal impairment.

Metformin, a bicyclic aniline drug, has been shown to have no action to increase insulin secretion in the pancreas, though its mechanism of action is not accurate, and it reduces glucose synthesis in the liver. BG analogue drugs have lower blood glucose lowering effects than sulfonylurea drugs, but are less likely to cause hypoglycemia. However, the incidence of gastrointestinal adverse events is high, and nausea, vomiting, diarrhea, and rash appear at the beginning of treatment, and lactic acidosis is caused to cause life-threatening fatal side effects. have. Thiazolidinedione drugs have recently been developed and have the effect of improving insulin resistance.

In addition, there are acarbose-based drugs that interfere with the digestion of carbohydrates after ingestion of carbohydrates, thereby hindering the rise of blood sugar. The acarbose drug reversibly inhibits alpha-D-glycosidase activity as an alpha-glucoamylase inhibitor and is known to inhibit the activity of maltase present in the mucosa of small intestinal cells. However, it is known that acarbose has a weak inhibitory effect on maltose degradation and strongly inhibits the decomposition of sugar.

Meanwhile, currently used herbal preparations are prescribed differently according to the place of diabetes. Ginseng Baekhotang (gypsum, radish, ginseng, licorice), which has a fever in the lungs and has a red tongue and a severe thirst, releases the heat of the lungs (淸 肺 熱), does not sweat the spleen, , Gwangseong Baekutang (ganggangguk, ginseng, Baekwol, Baekbokryeong, Mokhyang, Gwacheon, licorice) and Kangsimang (降 心 汤) prescribe.

There is heat on the stomach, and the stool is stiff and the stool is stiff. However, the stomach is not always cool. Etc.) and pure ki (prescription).

There is a lot of heat in the gods, so there is a lack of renal water, and the juice is dry and the thirst less. However, Prescribe.

However, when diabetes develops, appeal, small size, and calcination may occur separately, but in most diabetic patients, these three diseases occur at the same time. Therefore, most of the commonly used herbal preparations can not effectively treat diabetes mellitus suffering from the same disease.

In addition, it is known that reducing insulin resistance, increasing insulin secretion, inhibiting carbohydrate digestion can improve diabetes, and strictly controlling blood glucose can prevent or delay the occurrence of diabetic complications. However, Drugs that are effective in the treatment of diabetes are lacking in strict blood sugar control and prevention of diabetic complications, and development of new therapeutic agents is required.

Accordingly, the inventors of the present invention completed the present invention upon confirming that it has an effect of enhancing insulin action in extracts of chrysanthemum powder, Puerariae radix, Bacillus subtilis, Omija, licorice root, Waiin, jujube and jujube while studying substances which increase insulin secretion in herbal medicines.

As a conventional method for preparing the herbal extract, a herbal cosmetic composition comprising a natural plant extract derived from chestnut flower of Patent No. 10-0930122, a method for producing the composition, and a natural herb extract of Registration No. 10-1145498 The methods used for the extraction of herbal medicines in the prior art, such as in the bodywash manufacturing method and its body care compositions, include distillation, solvent extraction, membrane separation, crystallization, evaporation and adsorption, Is a method of utilizing the difference in volatility between the respective materials, and in the solvent extraction method, there is a separation method that utilizes the difference in solubility and the difference in the permeability of the membrane.

Distillation is employed in the case of separating substances with similar vapor pressures or nonvolatiles and requires a lot of energy. This method is disadvantageous in that it is difficult to process pyrolysis and heat denatured materials such as herbal medicines at high temperature, and the solvent extraction method has a problem in that the extraction cost is high by using a large amount of toxic solvent, And the membrane separation method is disadvantageous in that it is difficult to obtain a high purity extract due to polarization polarization.

SUMMARY OF THE INVENTION Accordingly, the present invention was conceived to solve the above-mentioned problems, and it is an object of the present invention to provide a herbal medicine extract obtained by concentrating an extract using a CO 2 or N 2 carrier gas and concentrating it in a high concentration syrup or jelly form, It is possible to maximize the antioxidative effect due to enzyme activity, low oxygen solubility and high viscosity, and to have a very slow change characteristic in the atmosphere after extraction without changing physical properties, taste, incense and color, And a method for producing the same. The present invention has been accomplished on the basis of the technical object of the present invention.

In order to accomplish the above object, the present invention provides a method for producing a starch, comprising the steps of: 10 to 20 wt% of silkworm, 5 to 10 wt% of Puerariae radix, 5 to 10 wt% of raw persimmon, 5 to 10 wt% %, And jujube of 5 to 10% by weight, based on the total weight of the composition.

The present invention also relates to a method for preparing a fermented soybean paste comprising 10 to 20% by weight of a silkworm powder, 5 to 10% by weight of a gypsum powder, 5 to 10% by weight of raw persimmon, 5 to 10% And 10% by weight based on the total weight of the herbal composition, and extracting and concentrating the pulverized herb medicine with a supercritical extraction method using CO2 or N2 carrier gas.

The method for preparing a herbal tea extract composition according to the present invention and the method for preparing a carbonated beverage and a health drink using the herbal composition and the supercritical extraction method according to the present invention are characterized in that the herbal medicine extract does not destroy any vitamins and amino acids, It is possible to maximize the antioxidant effect due to solubility and high viscosity, and to change slowly even in the atmosphere after extraction without changing the physical properties, taste, flavor, and color, so that the blood glucose controlling effect and the anticancer effect are greatly improved .

Fig. 1 is a view showing a process for producing a herbal tea extract composition according to the present invention
2 is a schematic view of an extractive concentrator according to the present invention
FIG. 3 is a graph showing an example of expected supernatant data of the extraction concentration temperature according to the present invention
4 is a graph showing an example of comparative experimental data of a carrier gas concentrator according to the present invention and a conventional separator
5 is a graph showing the state of temperature and molar fraction in the extractive concentrator according to the present invention
FIG. 6 is a graph showing the internal temperature change state of the extraction concentrator according to the present invention

The method for preparing the herbal composition for the herbal tea of the present invention and the method for preparing the herbal tea extract composition by the supercritical extraction method and the method of manufacturing the carbonated beverage and the health drink using the same will be described in more detail with reference to the accompanying drawings.

1 to 6, the method for preparing a herbal tea extract composition by supercritical extraction comprises the steps of: mixing the composition of the present invention Is extracted and concentrated by using a CO 2 or N 2 carrier gas to prepare a pulverized herbal medicine pulverized product.

The efficacy of the herbal medicines described above is as follows.

Trichosanthis Radix is a perennial vine herbaceous roots that have been peeled off from Trichosanthes kirilowii Maximowicz or Trichosanthes kirilowii Maximowicz var. Japonica Kitamura (bark and Cucurbitaceae). Ingredients include a large amount of starch, steroids (? -D-glucopyranosyl-stigmast-7-en-3β-ol), triterpenoids (3β, 24,25-trihydroxycucurbit- di-hydroxycucurbitacin B, D), citrulline,? -aminobutyrate, trichosantic acid, and the like. Effective for fever, eczema, and swelling. Seeds can be used to cough, asthma can be controlled, blood sugar is reduced, and has the effect of stopping the thirst.

Puerariae Radix is made by cutting off the roots of Pueraria thunb. Belonging to Leguminosae and cutting off the cork. Daidzin, Puerarian, starch And so on. [Divine Maternity] states that it controls poisoning, fever of the whole body, vomiting, paralysis, causing poisoning, and detoxifying various poison. It is said to cure, and there is an action to stimulate and lift up the nasal mucous membrane (sun).

Rehmanniae radix is a rhizome of Rehmannia glutinosa Libosch. Scrophulariaceae plant. Its main components are Catalpol, Verbascose mannitol, Glucose, Mannotriose, and vitamin A (vitamin A). It is cold, flavorless, poisonous, free of all heat, breaks blood, moans, cheeks and blood, and is used to control blood (blood).

Schizandrae Fructus is a deciduous broad-leaved vine, with leaves alternating with ovate or obovate, with sharp tips, sharp teeth, and slightly hairs on the back side. The flower is reddish white, bloomed in June-July, and it is migratory. Fruit is used for food, hangs in the shape of a spike at the end of the branch, and ripens red from August to September. The fruit is called Schizandrae Fructus and Schizandrae Fructus. 200 ~ 1,600 m above sea level. It is the main province of Jirisan, Jeonbuk and Gangwon provinces. It is wild throughout the country except Chungnam and Chungbuk, and is distributed geographically in Japan, Sakhalin, Manchuria and China. Fruits include Schizandrol, Schizandrin A, B, C, angeloylgomisin O, P, epigomisin, pregomoisin, deoxyschizandrin ) And the like. Omija has long been used as a raw material for medicinal herbs because of its medicinal properties such as astringency, nourishment, tenderness, astringency, ginger poison, thirst, astringency, Human stability due to ingestion has already been confirmed.

Licorice (Glycyrrhizae Radix) is a perennial herb with rosemary roots of dicotyledonous plants. Its ingredients are warm and tasty. It antagonizes various polar drugs and toxic drugs and treats poisoning caused by poison or poison. It also neutralizes and detoxifies poison caused by. In addition, like corticosteroids (补 Renal Cortical), such as adjustment to work to release the symptoms of tension and muscles to relieve pain and nervous tension, Glycyrrhizin (Glycyrrhizin) and anti-allergic action, gastric ulcer , And duodenal ulcers. In addition, decholesterol action to prevent arteriosclerosis, toxic substances to detoxify the function of the liver is strengthened. In addition, there was a clear therapeutic effect on pleural inflammation and pulmonary tuberculosis, and there was a clear effect on pituitary insufficiency, Edison's disease, epidemic hepatitis, bronchial asthma, dermatitis (urticaria, eczema, acne and freckles), schizophrenia, bronchitis, Therapeutic effect.

Cocicis Semen is a mature seed from which the seed coat of Gramineae has been removed and its main components are proteins, fats, carbohydrates, starch, amino acids, vitamin B1, coixol, Coixenolide, and the like. It is used for inflammation, drainage, diuretic and analgesic in oriental medicine. It is used for body pain, shoulder pain and roughness. It is also used for edema and wart removal. It is also used as a tonic for patients with pulmonary tuberculosis and pleurisy. In the private sector, the fruit itself is used medicinally, and the fruit of similar roses is also used.

Jujube is a foliage of deciduous broad-leaved fragrant Zizyphus in Rhamnace (Rhamnace). It is known that there are about 40 varieties and 400 varieties. Origin is North Africa and Western Europe and its main origin is China. Leaves are elliptical, smooth, and three veins are distinct. They are elliptical, with thin shells, green, and reddish brown.

The pulp has no fragrance, and has a strong sweetness and acidity, giving a refreshing feeling. The jujube has a red color and has been put on the road and the ceremony ceremony of Korea. It is edible through reproduction and cooking, and the biodegradation is mainly used because it is low in storage stability. From a long time, jujube has often been added to sweeten the flavor of liquorice in one room. insomnia. It was used for medicinal purposes because it had the effect of improving symptoms such as indigestion, colonic hemorrhage, cough, and hypersensitivity.

As a medicinal component of jujube, various sterols, alkaloids, saponins, vitamins, serotonin, organic acids, fatty acids, polyphenols, hydroxymethylpuppural, flavonoids and amino acids have been reported. And the jujube has been used as a digestive emollient and tonic for many thousands of years as a herbal medicine. In the private sector, it has been known to dry the ripe jujube, and when it eats for a few months, it eats heat and dilutes the stool to eliminate constipation and stop coughing. If you have an arousal action and roasted, hypnotic action is said. The jujube has been considered as one of the top preservatives in the oriental medicine because it neutralizes all kinds of poison, eliminates the inflammation of the body and strengthens the fragile constitution, and the jujube has been regarded as one of the most important supplements. In the era when pollution poisoning floods and over- And it is a very suitable food for keeping the taste of our nation.

Jujube has many medicinal effects such as nourishment, interpenetration, tonic, facial facial and poisoning, inhibition of cancer cell proliferation, reduction of cholesterol, treatment of non platelet reducing purpura, treatment of chinhae and gaejungdam, treatment of chronic bronchitis, treatment of bacterial dyslipidemia, Anti-allergic action, muscle contraction, enhancement of liver function, etc. As a result of recent clinical studies, the functional and therapeutic importance of jujube has been recognized.

Jujube is strong in sweetness and contains about 10-20% of soluble sugars. Its main sugars are fructose glucose and sucrose, and contains various flavor components.

In the following, we will explain in more detail the experiment that can grasp the functional and therapeutic importance of jujube.

1. Preparation and composition analysis of jujube extract

end. Experimental material

The jujube used in this experiment was selected and washed with dried demineralized (福 夏) varieties produced in Gyeongsangbuk-do. The jujube was extracted with a mixture of 25% jujube and 75% water at 110 ~ 20 ℃ for 3-6 hours. Jujube extract was used

I. General composition, sugar content, pH and color

The general components of the samples were measured according to the food engineering experiment method, and the sugar content was measured with a refractometric meter (Atago, Japan) and the pH was measured with a pH meter (Hanna, U.S.A.). The color of the jujube extract was diluted 10 times with distilled water and filtered through a 0.45 μm membrane filter. The absorbance of the solution was measured at 500 nm using a spectrophotometer (Pharmacia, Sweden) and then multiplied by the dilution factor.

All. amino acid

Amino acid analysis was performed by mixing a certain amount of sample with 6N-HCl solution, filling with nitrogen, hydrolyzing at 110 ° C for 24 hours, and then diluting with 50 ml. This was filtered through a 0.45 μm membrane filter, and 20 μl of the filtrate was vacuum dried. To the dried sample, 30 ㎕ of methanol: water: triethylamine (2: 2: 1) solution was added and dried. V) was added, and the mixture was allowed to stand for 20 minutes, followed by tertiary drying. 30 μl of methanol was added, dried again and re-dissolved in sodium acetate buffer (pH 6.4). This was analyzed using HPLC (High performance liquid chromatography, Waters Associates Inc., USA)

la. Minerals

Mineral analysis was carried out by dry painting method. That is, the sample was stirred at 550 ° C. for 4 hours and then dissolved in 0.2 N HNO 3 solution, and the solution was filtered with 100 mL. For the analysis, ICP (Inductively coupled plasma, Jobin-Yvon Model JY 38 Plus, France) was used.

hemp. Phenolic material

Analysis of the phenolic material was carried out by modifying the Folin-Denis method. 0.1 mL of the sample solution and 2 mL of 2% sodium carbonate were mixed. After 2 minutes, 0.1 mL of 50% Folin and Ciocalteu's phenol reagent was added, and the mixture was allowed to stand at room temperature for 30 minutes. Then, the mixture was treated with a UV-VIS spectrophotometer (Shimadzu UV- ㎚. The standard solution was chlorogenic acid.

bar. Non-volatile organic acids, free sugars

The column was DB-FFAP (0.53 mm ㅧ 30 m) and the column temperature was 100 캜 (5 min) -4 캜 / min-220 캜 ( 5 min), inlet temperature 230 [deg.] C, detector FID) temperature 250 [deg.] C, and carrier gas was analyzed with nitrogen (2 ml / min).

The free sugars were analyzed by HPLC method after removal of ionic substances by using TMD-8 (Sigma, USA) as a resin. Rezex RNM and RPM (7.8 ㅧ 300 mm, Phenomenex, USA) were used for the separation column, and the flow rate of ultrapure water was 0.6 mL / min for the mobile phase. The column temperature was 75 And detector was Shimadzu RID-6A.

2. Measurement of physiological activity of jujube extract

end. Electron donating ability measurement

The electron donating abilities (EDA) of jujube extracts were measured by the modified method of Blois (66). 1.0 mL of 2 × 0-4 M α, α-diphenyl-β-picryl-hydrazyl (DPPH) was added to 1.0 mL of each diluted sample and allowed to stand for 30 minutes. Absorbance was measured at 517 nm . The electron donating effect was expressed by the absorbance reduction ratio when the sample was not added.

Electron donating ability% = (1 - (absorbance of sample added sphere) / absorbance of no added sphere) x 100

I. Xanthine Oxidase inhibitory effect

Xoase activity inhibition was measured by Stirpe and Corte method. In other words, 0.1 mL of the enzyme solution (40 mU Xantine Oxidase) and 0.1 mL of the sample solution were added to 1 mL of the substrate solution in which 2 mM of xanthine was dissolved in 0.1 M potassium phosphate buffer (pH 7.5), 0.1 mL of distilled water was added to the control sample The reaction was terminated by the addition of 1 mL of 1 N HCl, and the uric acid produced in the reaction solution was measured at 292 nm. The inhibition rate was determined by the following equation.

Inhibition rate% = (1 - uric acid in the reaction) / uric acid in the control × 100

All. Tyrosinase inhibitory effect

Tyrosinase activity inhibition assay was performed according to the method of Yagi et al., Which measures DOPA chrome produced by the action of tyrosinase by colorimetry. 0.5 mL of the extraction solution was added to a mixture of 0.1 mL of Mushroom tyrosinase (110 units / mL), 0.2 mL of 5 mM L-DOPA as a substrate and 0.2 mL of 0.175 M phosphate buffer (pH 6.8), and the mixture was reacted at 35 ° C. for 2 minutes, DOPA chrome was measured at 475 nm and the inhibition rate was calculated by the following equation.

Inhibition effect (%) = (1 - SAbs - BAbs) CAbs / 占 100

here,

SAbs: Absorbance at 475 nm determined with test sample

BAbs: Absorbance at 475 nm determined with buffer instead of enzyme

CAbs: Absorbance at 475 nm determined with buffer instead of test sample

la. Metal ion blocking action

Metal ion blocking activity was measured by varying the method of Buege and Aust. 1 mL of the reaction mixture was filled with metal ions and a sample and reacted in a water bath at 37 ° C for 1 hour. As soon as the reaction was completed, 50 μl of dibutylhydroxy toluene (BHT) was added to the sample to stop the oxidation reaction. The reaction mixture was mixed well, then 2 mL of TCA / TBA reagent was added, mixed again and heated in boiling water for 15 minutes. After heating, the mixture was cooled in cold water and centrifuged at a rate of 2,000 g for 15 minutes. The absorbance of the supernatant was measured at 531 nm and the inhibition rate was measured by the same method using distilled water instead of the sample

Metal ion blocking capacity%) = (1 - absorbance of sample added solution) / absorbance of non-added solution x 100

hemp. Cancer cell proliferation inhibitory effect

(1) Cell culture

Cell lines MDA-MB-231 (breast cancer, KCLB 30026), SK-MEL-31 (Malignant melanoma, KCLB 30073), G361 (Melanoma) and A549 (lung cancer, KCLB 10185) KCLB). Each cell was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 100 units / mL penicillin / streptomycin 1%, and cultured at 37 ° C in a 5% CO 2 incubator.

(2) MTT assay

The proliferation inhibitory effect of jujube extract on cancer cell lines was determined by MTT assay according to the method of Charmichael et al. The cells were cultured in a 37 ° C, 5% CO 2 incubator for 48 hours, and the control group was treated with the same amount of distilled water as the sample And cultured under the same conditions. To this was added 20 쨉 l of a solution of MTT (N-methylthiotetrazol) prepared at a concentration of 5 mg / ml and further cultured for 4 hours. After completion of the incubation, the culture medium was removed, and 150 μl of DMSO was added to each well. After stirring for 30 minutes, the absorbance at 550 nm was measured with an ELISA reader to measure the inhibitory effect of cancer cells. The inhibitory effect on cell proliferation was calculated by the following equation.

Growth inhibition effect (%) = Absorbance of control - Absorbance of sample treatment / Absorption of control × 100

3. Analysis of Physicochemical Components of Jujube Extract

end. General component

Contents of flour and jujube extract were 90.52% moisture content, crude protein content 0.50%, ash content 0.37%, sugar content 10.Brix and pH 4.65. The content of phenolic compounds in jujube extract was 22.82mg%.

 I. Free sugar, organic acid

The free sugars of jujube extracts were sucrose, fructose and glucose. The content of sucrose was 4,340 mg%, glucose 3,910 mg% and fructose 1,360 mg%.

The content of levulinic acid was 295.69 mg%, malic acid and oxalic acid were 36.13 and 32.88 mg%, respectively, and citric acid, lactic acid and glutaric acid were abundant in the nonvolatile organic acids of jujube extract. Respectively.

All. Minerals

The contents of potassium and calcium of jujube extract were 12,425ppm and 314.72ppm, respectively. The contents of potassium, magnesium, and calcium were the highest in jujube extract, and the contents of iron, manganese, and zinc were small.

4. Physiological activity and proliferation inhibition effect of jujube extract

end. Electron donating ability measurement

Free radical scavenger function is one of the important antioxidant characteristics. DPPH can be measured by free radical scavenger ability of antioxidant by reacting with antioxidant as free radical. Therefore, in this experiment, the electron donating ability of jujube extract was measured using α, α'-diphenyl-β-picrylhydrazyl (DPPH) as free radical. 10.Brix jujube extract showed high antioxidative effect of 86.50% and antioxidative effect of 5.Brix jujube extract diluted twice with distilled water also showed high antioxidative effect of jujube extract.

I. Xanthine oxidase Inhibition

Xanthine oxidase is an enzyme involved in purine metabolism in vivo and forms an element from xanthine or hypoxanthine. When the element is accumulated in the plasma, it accumulates in the fracture, so it causes not only painful gout but also kidney disease, 10. Inhibition effect of xanthine oxidase inhibition of Brix jujube extract and double-diluted 5.Brix jujube extract was 72.78% in juice of 10.Brix juice and 53.40% in juice of juice juice of 5.Brix juice .

All. Tyrosinase Inhibition

The tyrosinase inhibitory effect of melanin was found to be 10.23% inhibition effect in 10Brix jujube extract and 10.43% inhibition in 5.Brix jujube diluted twice in distilled water.

la. Metal ion blocking action

The Fe2 + ion binding capacity of the oil emulsion containing jujube extract was 41.21% in the 5.Brix jujube extract and in the 5.Brix jujube diluted with distilled water, respectively, as measured by Fe2 + and Cu2 + And 24.05%, respectively. In the Cu2 + ion binding ability, the binding ability was shown to be 62.65% and 36.0% in the 5.Brix jujube extract and the 5.Brix jujube diluted twice with distilled water, respectively.

hemp. Cancer cell proliferation inhibitory effect

10. Brix jujube extract and two-fold diluted 5.Brix jujube extract were used for MDA-MB-231 (breast cancer), SK-MEL-31 (malignant melanoma), G361 (melanoma) and A549 And the inhibitory effect of each cancer cell proliferation was observed.

MDA-MB-231 (proliferation inhibition test on mammary tumor cell lines) showed excellent proliferation inhibitory effect of 92.95% and 91.96% in 10.Brix jujube extract and 5.Brix jujube diluted twice with distilled water. SK-MEL 31 (In the inhibition test of malignant skin cancer cell tumor cell proliferation, the growth inhibition effect was also excellent 93.64% and 92.02% in the 5.Brix jujube extract and 2-fold diluted 5.Brix jujube extract of distilled water. A549 (In the case of lung cancer cell cancer, 10.Brix jujube extract showed 95.64% proliferation inhibitory effect in G361 cancer cells and 5.Brix jujube diluted with distilled water showed 94.70% proliferation inhibitory effect.

In the A549 cancer cell, the 10.Brix jujube extract showed an excellent proliferation inhibitory ability of 95.41%, whereas the 5.Brix jujube extract diluted twice with distilled water showed a proliferation inhibitory ability of 55.32%. The jujube extract inhibited the proliferation of all four cancer cell lines. The extracts of jujube showed excellent proliferation inhibitory effect of the cancer cells with increasing concentration. As a result, it was confirmed that the jujube extract's excellent proliferation inhibitory effect on the cancer cell line was confirmed. However, it is necessary to confirm the effect of the substance on the cancer cell line and isolate and purify the substance, .

The above-mentioned oriental medicinal herb has 10 to 20 wt% of silkworm, 5 to 10 wt% of Puerariae radix, 5 to 10 wt% of raw persimmon, 5 to 10 wt% of Omija, 5 to 10 wt% of licorice, 20 to 50 wt% 10% by weight. Such a composition ratio has been found in consideration of the effective dose and side effects of each herbal medicine component, and when the ratio is out of the range, the effect is drastically decreased or there is a fear of side effects.

The herbal herb medicine is used as a herbal composition, and is extracted and concentrated through a CO 2 or N 2 carrier extractor. The supercritical extraction method can be extracted at low temperature and in a short time. Therefore, it is the most effective extraction method. It has three states of solid, liquid and gas due to the nature of the material. When it changes into one of three properties by temperature or pressure Among the three properties, it is a method to extract when it becomes a supercritical state, not a liquid or a gas.

The supercritical extraction is an extraction method using a fluid above a critical point as an extractant. Since the supercritical fluid has excellent solubility for many materials and can change the solubility of the substance by simply controlling the temperature and pressure, It is used as a field of technology. Particularly, since CO₂ has a critical temperature of 31.06 ℃, it can be extracted at a low temperature and can prevent damage to the extracted active ingredient by heat, and it can reduce the energy cost during the process. Therefore, it is used as supercritical fluid solvent do.

As shown in FIG. 1, the method of manufacturing the herbal tea extract composition using the CO 2 or N 2 carrier extractor comprises the steps of: (1) purchasing raw materials of herb medicine such as chrysanthemum, chrysanthemum, persimmon, After drying at 60 ° C, it is cut to 0.1 to 0.2 cm in a pulverizer, and then the cut herbal medicine is charged into a CO 2 or N 2 carrier extractor for extraction. Then, the extracted extract is packed in a bottle or pack, and sterilized and packaged at 40 to 90 ° C to be tested and shipped. At this time, the concentrator used at the same time as the CO2 or N2 carrier extractor has cooling, circulating and stirring effect.

The extraction and concentration technique using the CO 2 or N 2 carrier gas used for extracting the herbal medicine as described above is one of the most important processes in the production process of the product and can be used in various processes in a chemical process or a laboratory, The system can effectively separate substances with weak heat or mixtures with high evaporation points using carriers.

The extraction and concentration system using the CO 2 or N 2 carrier gas in the present invention can prevent the modification of the thermally unstable compound by lowering the vapor pressure of the solvent by circulating the carrier gas in the system instead of the vacuum pump, By circulating in a closed circuit space, it is possible to produce a product having a unique medicinal ingredient, fragrance and taste inherent to herbal medicinal herb extracts which are easy to volatilize even at low temperatures, and the concentration of the extract can be controlled according to application conditions.

Hereinafter, the present invention will be described in detail with reference to the drawings.

2 is a schematic diagram of an extractor concentrator according to the present invention in which circulation of inactive CO 2 or N 2 through a pump 16 to a confined space of the system lowers the vapor pressure of the concentrate portion located in the heat exchanger 14, The containing carrier migrates to the cooler 12 portion where the condensation takes place and is collected in the vessel 17. After the condensation, the dried CO 2 or N 2 is recycled through the pump 16.

FIG. 3 is a graph showing an expected boiling point data of the extraction condensation temperature according to the present invention. When 200 ml of water is used as a carrier gas, boiling point enhancement effect is shown. It was observed that the boiling point was 56 ° C when the gas volume was 15 m 3 / h. As a result of the experiment, it was confirmed that water can be distilled under optimum conditions when the heating temperature is 60 ° C and the gas volume is 15 m 3 / h.

4 is a graph showing comparative experimental data between a carrier gas concentrator according to the present invention and a conventional reduced pressure concentrator. As shown in FIG. 4, when the gas volume is 10 m 3 / h at a low gas volume of 8 m 3 / h, Indicating a 20% increase in resolution over reduced pressure distillation. The optimum condition was increased to 80% with 15 m3 / h (gas volume). However, when the maximum gas volume (20 m 3 / h) is used, the efficiency of the separation is lowered because the common material is mixed in the condensate.

FIG. 5 is a view showing the temperature and the mole fraction in the extracting concentrator according to the present invention. The concentration is an operation for separating the solute and the solvent having a relatively large proportion, and can be compared with the stripping of the distillation. Distillation is an operation to purify and remove a low-boiling, multi-component solution with a low boiling point and a high boiling point material. For this purpose, the liquid phase and vapor phase equilibrium relationship of the components should be determined. The X value on the line represents the liquid fraction of the low boiling point material, and the Y value represents the gas phase fraction of the low boiling point material. (X0-X1) / (Y1-X1) ratio is vaporized according to the reverse bar scaling formula, and the remaining portion is evaporated by heating the solvent fraction X0, temperature T0 solution to be separated by the solvent fraction Y2 and temperature T2 vapor, Liquid equilibrium. At this time, the solvent fraction of the liquid phase becomes X1, the solute is concentrated, the solvent fraction of the gas phase becomes Y1, and the solvent is purified. In this way, the distillation column has a plurality of equilibrium stages in which the low-temperature liquid phase and the high-temperature gase-phase are contacted in opposite directions and the evaporation condensation is repeated to concentrate (remove) the boiling point material and purify the low boiling point material.

FIG. 6 is a state diagram showing the internal temperature change of the extracting concentrator according to the present invention. The concentration of the concentrate was maintained at a constant level after 20 minutes of operation and showed a solvent evaporation rate of 2 L / h. After 2 hours, And the temperature slightly started to rise. Under the same conditions, it was not able to be decompressed any more because of the boiling phenomenon of the concentrate at 400 mbar, and the evaporation amount of the solvent was 1.2 l / h, and the efficiency of the carrier gas concentrator was 60% in terms of evaporation amount.

The extraction concentrator used in the present invention is a device for heating a herbal medicine with a solvent (ethanol, water) as a main raw material at a constant temperature and pressure, and a soluble liquid which is extracted is called an extract. (Or ethanol) which is evaporated by keeping at a constant temperature by atmospheric pressure or decompression to increase the concentration if necessary, is recycled by evaporating and cooling by introducing the water (or ethanol) into the cooling device, and the remainder Concentrate).

In the present invention, herbal medicine is extracted with CO 2 or N 2 under low temperature and vacuum, and the extract is concentrated to concentrate in high-concentration syrup or jelly form, so as not to destroy any vitamins or amino acids, And generally, the physical properties, taste, aroma and color are not changed at all, and the extract is changed very slowly even in the atmosphere after extraction. The characteristics of the general extraction concentration process are as follows.

The antimicrobial and enzymatic effects are more effective than the Open Pan evaporator. 50 to 60% of extracted concentrates are monosaccharides and acids with very strong osmotic pressure, and products containing an amount of sugars with weak osmotic pressure can be extracted and concentrated to 65 to 70% of solids.

In case of high sucrose content such as licorice, it can be heated up to the evaporation temperature in order to make jam or syrup. Pine needles can be extracted and concentrated by concentrating 16 ~ 28% Additional processing is possible, and ginseng can be concentrated like open pan or vacuum concentrator. At this time, the same quality change as the Maillard reaction by heating is also possible. This extraction concentration evaporation process is carried out under reduced pressure, at 60 ° C or lower. In addition, high extraction and concentration are performed at minimum pressure and maximum temperature for extractive concentration without changing the physical properties of herbal medicine. At this time, since the concentration and viscosity gradually increase during the concentration process, it is difficult to cool the reaction heat. Therefore, stirring is performed and extraction concentration is performed.

The carrier gas concentrator used in the present invention is made of a reaction furnace and the concentrate inlet of the upper portion is made of tempered glass. The inside of the concentrator can be observed with the naked eye and the lid is closed with a silicone ring to prevent leakage of the circulating gas , A material capable of suppressing the reactivity of the concentrate is used. A stirrer, a heat exchanger, a control box, a heater, an operation key, a stirrer, a cooler, and a circulation pump controller are attached to the upper side of the reaction furnace, so that all the processes are automated, thereby allowing easy manipulation. The separator is a device for collecting the solvent of the concentrate. A glass tube is installed on the side of the separator to easily measure the level of the solvent, and the recovered solvent can be recovered through the valve provided at the lower end. The water tank is a place for storing the refrigerant of the heat exchanger, and the temperature of the refrigerant can be adjusted to 8-15 ° C, and the solvent exchange ratio of the refrigerant circulating device is increased to enhance the concentration efficiency.

(Aerosol Test) In order to examine the azeotropic point of the concentrator to operate as the maximum amount of the circulating gas, 10 liters of distilled water was added to the concentrator, and when 0.1 g of Fluorescein was added, the color became greenish . After the reactor was operated at a temperature of 50 ° C, a stirrer rotation speed of 60 rpm, a cooler temperature of 15 ° C and a gas circulation pump of 15 m 3 / h for 2 hours, the solution in the separator was sampled and judged as a color with a spectrophotometer. In this experiment, no color appeared in the collected solution, and it was found that there was no particle transition when the amount of gas was maximum.

The herbal medicine herbal extract prepared according to the present invention as described above maximizes the antioxidant effect due to enzyme activity, low oxygen solubility, and high viscosity without any destruction of vitamins, amino acids, etc. which are active ingredients of herbal medicine And can change very slowly even in air after extraction without changes in physical properties, taste, aroma, and color.

In the following, the embodiments will be described in more detail.

≪ Example 1 >

10 to 20 wt% of silkworm, 5 to 10 wt% of Puerariae radix, 5 to 10 wt% of raw persimmon, 5 to 10 wt% of Omija, 5 to 10 wt% of licorice, 20 to 50 wt% Herbal medicine powder was mixed and extracted and concentrated using a CO2 or N2 carrier gas to obtain a herbal medicine extract.
50 to 80% by weight of the herbal tea extract, 5 to 20% by weight of sugar, 0.05 to 0.3% by weight of citric acid, 0.005 to 0.02% by weight of caramel, 0.1 to 1% by weight of vitamin C and 79 to 94% The syrup is sterilized at 85 to 98 ° C for 20 to 80 seconds and mixed with the cooling water at a ratio of 1: 3.5 to 4.5. Then, the carbonic acid gas is injected into the carbonated beverage .

In one embodiment, the herbal medicinal herb extract is contained in an amount of 50% by weight, and an additive of 5% of sugar, 0.1% of citric acid, 0.02% of caramel and 0.5% of vitamin C is mixed therewith, % Of purified water to make a syrup and sterilize the syrup at 85 ~ 98 ℃ for 20 ~ 180 seconds, mix with cooling water at a ratio of 1: 4, then inject 0.5 ~ 0.82% carbon dioxide gas, Composition carbonated beverages were prepared.

(Test Example: Prevention of Blood Sugar and Measurement of Hair Growth Effect)

Fifty subjects with blood sugar were selected and used twice a day using the extract obtained in Example 1 above.

As a result, blood glucose was significantly reduced in 48 patients using 50 patients who had symptoms for more than 1 month continuously. From these results, it has been observed that the patients suffering from hyperglycemia decreased from 2 to 3 days after taking the extract without taking the blood glucose drug, and the hyperglycemia phase was almost stopped from the use of about 20 days. Therefore, the constitution, , But the effect was satisfactory in general.

≪ Example 2 >

Oriental herbivores were purchased from the Daegu Yakimyeon market, washed cleanly with water, and dried in the shade. One kilogram of the dried sample was added to 10 liters of 70% ethanol and extracted twice for 2 hours. The ethanol extract solution was filtered with a filter, centrifuged at 2000 rpm, and the supernatant was concentrated under reduced pressure in a rotary vacuum concentrator and lyophilized to prepare an extract powder sample.

The extracted powder sample was suspended in water, and then methanol was added to increase the volume. The column was packed with Amberlite XAD-4 gel (Amberlite XAD-4 gel) while varying the polarity of the eluent to obtain a water fraction ), 20% methanol fraction (Fr.2), 40% methanol fraction (Fr.3), 60% methanol fraction (Fr.4), 80% methanol fraction (Fr.5) and 100% methanol fraction ), And each fraction was concentrated under reduced pressure at low temperature, and then freeze-dried. Each fraction was dissolved in DMSO at 1 mg / ml and used.

(Experimental Example 1: Effect of the extract of the present invention on glucose uptake into fat cells)

In order to examine the effect of the extract of the present invention on glucose uptake into adipocytes, the following experiment was conducted.

1. Cell culture

The 3T3-L1 cells used in the experiments were purchased from a US cell line and cultured in DMEM supplemented with 1% FBS (fetal bovine serum), gentamycin (100 units / ml), streptomycin (100 μg / (Dulbecco's modified Eagle's medium).

The 3T3-L1 fibroblasts were washed with phosphate buffer solution at 3 days intervals, and then 1 ml of 0.25% trypsin-EDTA solution was added per 50 ml flask. The solution was treated at room temperature for 1 minute, and then the trypsin solution was discarded. For 5 minutes, and cells were desorbed and subcultured. The desorbed cells were suspended in 10 ml of DMEM supplemented with 10% FBS, transferred to a new culture container (50 ml culture flask) and incubated in a CO₂ incubator (37 ° C, 5% CO2) at a split ratio of 1:20, Lt; / RTI >

2. Glucose uptake into fat cells

3T3-L1 fibroblasts were cultured in the same manner as the above subculture until they were completely confluent. Two days after the bottom of the culture vessel was completely filled, 0.25 μM of dexamethasone, which is an inducible differentiation material, was added for 3 days. Then, the cells were changed into high-glucose DMEM solution every 2 days for 10-15 days Glucose uptake test.

The number of adipocytes in the culture vessel was counted to dispense the wells of the 12-well plate so that they could be completely filled without voids. The adipocytes transferred to the well plate were washed with PBS and then cultured in HEPES solution for 30 minutes at 37 ° C in DMEM containing low concentration of glucose containing 1% bovine serum albumin for 12 hours or more . [14C] 2-deoxyglucose and 1 mM glucose were added at 1 μCi / ml and cultured at 22 ° C. for 30 minutes, and the degree of glucose uptake into the cells was measured by the amount of 14 C transferred into the cells.

In order to exclude nonspecific glucose uptake, the amount of glucose transferred into the cell was measured by subtracting the amount of glucose transport when cultured with cyto B (glucose uptake inhibitor of glucose transporter 4 (GLUT4)) .

The cells were washed with PBS containing 10 mM glucose and the cells were digested with 0.5 N NaOH. The disrupted cells were neutralized with acetic acid and the content of contained 14C was measured with a beta counter (Tri-Carb 2100TR, Packard Bioscince, IL).

3. Effect of the extract fraction of the present invention on glucose uptake

In order to investigate whether each fraction of the extract contained an insulin sensitizer, the extract fraction prepared in the above Example was dissolved in DMSO at 1 mg / ml, and the diluted fraction was diluted to 0.3 and 3 μg / ml in PBS Glucose uptake was measured at branching concentrations.

3T3-L1 adipocytes were incubated with insulin at 1 ng / ml for 1 hour, and then the glucose uptake was compared with the results at 50 ng / ml insulin.

Insulin 0 (basal), 1, 3, 5, 10, 25, 50 and 75 ng / ml were used as controls.

All experiments were repeated 3 times, and the results were expressed as a ratio to the value of the insulin and omija extract fractions not treated.

In order to investigate whether the effect of increased glucose uptake in the extracted fraction is specifically a phenomenon exhibiting improved action of insulin or that the glucose is specifically acting as a detergent in cells to move glucose into cells, The extent of absorption of glucose was measured in the presence of only the extracted fraction without addition.

In order to exclude that the extracted fraction acts as a solvent regardless of the action of insulin to increase the glucose uptake by destroying the cell membrane, when the glucose uptake is measured with the extract fraction at 0 ng / ml of insulin, ) Was considered to have no effect as an insulin sensitizer even though it increased glucose uptake.

The results were expressed as mean ㅁ standard deviation. The degree of increase in glucose uptake in 3T3-L1 adipocytes compared with the control group of the extract fraction was determined by adding only 1 ng / ml of insulin, 1 ng / ml of insulin, And the values were measured by two-sample t-test. The significance test of all statistical procedures was set to α = 0.05.

When 3T3-L1 adipocytes were treated with 3 ng / ml of insulin and 0.3 μg / ml of the extract fraction of the present invention, the concentration of insulin in the 3T3-L1 adipocytes was 25 ng / ㎖, glucose uptake was increased with increasing insulin concentration. However, glucose uptake into the cell was not different according to insulin concentration at higher insulin concentration than 25ng / ㎖. In addition, 60% methanol fraction (Fr.4) and 80% methanol fraction (Fr.5) increased glucose uptake when 0.3 μg / ml extract fraction was treated with 1 ng / ml insulin. Especially, 80% methanol fraction (Fr.5) showed more increase of glucose uptake than that of 50ng / ㎖ of insulin.

When the amount of the extracted fraction was increased to 3 μg / ml and treated with 1 ng / ml of insulin, 20% ethanol fraction (Fr.2), 60% methanol fraction (Fr.4), 80% methanol fraction (Fr. 5) and 100% methanol fraction (Fr.6).

Especially, 60% methanol fraction (Fr.4) and 80% methanol fraction (Fr.5) showed more glucose uptake than insulin treated 50ng / ㎖. Therefore, it can be seen that insulin action is greatly improved in the 60% methanol fraction (Fr.4) and the 80% methanol fraction (Fr.5) of Omija.

At this time, it can be seen that the glucose uptake value in the presence of only the extracted fraction is similar to the base value, or rather low, so that the fraction acts as a solvent and does not nonspecifically increase the glucose uptake.

4. Changes in the amount of GLUT 4 present in the cell membrane

After 3T3-L1 adipocytes were transferred to 10 cm 2 plates, 3T3-L1 adipocytes were cultured for 12 hours in high glucose DMEM supplemented with 3% bovine serum albumin instead of plasma, followed by addition of 3 μg / ml of Omija extract After incubation for 5 h, 0, 1 or 50 ng / ml of insulin was treated for 5 min. Adipocytes were terminated with cold PBS and immediately resuspended in lysis buffer consisting of 20 mM Tris (pH 7.4) containing 2 mM EDTA, 137 mM NaCl, 1% NP40, 10% glycerol and 12 mM alpha-glycerol phosphate and protease inhibitor and the cells were eluted with lysis buffer.

The cell lysate was left in ice for 30 minutes and then the cell membrane was separated by Kotani et al. The protein content of the separated cell membranes was measured by the Lowry method and the content of glucose transporter 4 (GLUT 4) in the same amount of cell membrane protein was measured by immunoblotting method using an anti-GLUT 4 antibody (Chemicon, Temecula, CA) .

The results showed that the amount of GLUT 4 present in the cell membrane when treated with 3 μg / ml of 60% methanol fraction (Fr.4) and 80% methanol fraction (Fr.5) with 1 ng / And increased to the level of insulin 50 ng / ml. This suggests that the 60% methanol fraction (Fr.4) and the 80% methanol fraction (Fr.5) of the extract increased the insulin signaling system and promoted insulin action, resulting in an increase in the amount of GLUT4 in the cell membrane.

Therefore, the increase of GLUT4 in the cell membrane is closely related to the increase of glucose uptake.

(Experimental Example 2: Acute toxicity test for oral administration to rats)

In order to examine the acute toxicity of the composition of the present invention, an acute toxicity test was conducted as follows.

Acute toxicity tests were performed on 6-week-old specific pathogen-free (SPF) SD rats as experimental animals. The compositions of the present invention were each suspended in 0.5% methylcellulose solution and administered once at a dose of 1 g / kg / ml to animals of two mice per group. After the administration of the test substance, the mortality, clinical symptoms, and weight changes of the animals were observed, and hematological tests and blood biochemical tests were carried out, and autopsy was performed to observe the abnormalities of the abdominal organs and thoracic organs.

As a result, there were no clinically symptomatic or dead animals in all the animals to which the test substance was administered, and no toxic change was observed in weight change, blood test, blood biochemical test, and autopsy findings.

Therefore, the composition of the present invention did not exhibit any toxicity change even at 1 g / kg in rats, and it was judged that the oral LD50 was at least 1 g / kg or more. Examples of formulations for the composition of the present invention are illustrated below.

(Formulation Example 1: preparation of pharmaceutical preparation)

1. Manufacturing of powder

2g of extract, 1g of lactose

The above components were mixed and packed in airtight bags to prepare powders.

 2. Preparation of tablets

100 mg of extract, 100 mg of corn starch, 100 mg of lactose, 2 mg of magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

 3. Preparation of capsules

100 mg of extract, 100 mg of corn starch, 100 mg of lactose, 2 mg of magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

(Formulation Example 2: Preparation of food)

Foods containing the extract of the present invention were prepared as follows.

1. Preparation of cooking seasoning

Healthy cooking sauce was prepared from 20 to 95% by weight of the extract of the present invention.

2. Manufacture of tomato ketchup and sauce

0.2 ~ 1.0 wt% of the extract of the present invention was added to tomato ketchup or sauce to prepare health-promoting tomato ketchup or sauce.

3. Manufacture of flour food

0.5 to 5.0% by weight of the extract of the present invention was added to wheat flour, and a bread, a cake, a cookie, a cracker and a noodle were prepared by using this mixture to prepare a food for health promotion.

4. Manufacture of soups and gravies

0.1 to 5.0% by weight of the extract of the present invention was added to soups and gravies to prepare meat products for health promotion, soup of noodles and juices.

5. Manufacture of ground beef

10% by weight of the extract of the present invention was added to ground beef to prepare ground beef for health promotion.

6. Manufacture of dairy products

5 to 10% by weight of the extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

7. Manufacturing of wires

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh. Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a known method, and then they were prepared into powders having a particle size of 60 mesh by a grinder.

The extract of the present invention was concentrated under reduced pressure in a vacuum concentrator, dried by spraying and dried in a hot air drier, and pulverized to a size of 60 mesh with a pulverizer to obtain a dry powder.

The above-prepared cereal grains, seeds and dried powder of the extract of Omiza were blended in the following proportions. (30% by weight of brown rice, 15% by weight of barley, 20% by weight of barley), seeds (7% by weight of perilla seeds, 8% by weight of black beans, 7% by weight of black sesame seeds) (0.5% by weight), sulfur (0.5% by weight)

(Formulation Example 3: Preparation of beverage)

1. Manufacture of carbonated beverages

A mixture of 50-80% by weight of herbal tea extract, 5-20% by weight of sugar, 0.05-0.3% by weight of citric acid, 0.005-0.02% by weight of caramel and 0.1-1% by weight of vitamin C, Purified water is mixed with 20 to 50% by weight of a mixture of carbonated beverages to make a syrup. The syrup is sterilized at 85 to 98 ° C for 20 to 180 seconds, mixed with cooling water at a ratio of 1: 3.5 to 4.5, 0.5 to 0.82% of gas was injected to prepare a carbonated drink containing the extract of the present invention. At this time, it is preferable to mix 5 to 10% by weight of sugar, and mix syrup and cooling water at a ratio of 1: 4.

2. Manufacture of health drinks
A health drink containing 50 to 80% by weight of a herbal tea extract, 0.3 to 6.5% by weight of liquid fructose, 1 to 10% by weight of oligosaccharide, 1 to 10% by weight of sugar, 0.3 to 6.5% by weight of salt, and 68 to 87% 20 to 50% by weight of the mixture is uniformly blended and sterilized at a moment.
In one embodiment, 20 to 50% by weight of a supplementary material such as liquid fructose (0.5% by weight), oligosaccharide (2% by weight), sugar (2% by weight), salt (0.5% 50 to 80% by weight of tea extracts were uniformly blended and instant sterilized, and then packaged in small containers such as glass bottles and plastic bottles to produce health drinks.

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3. Manufacture of vegetable juice

5 g of the extract of the present invention was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice for health promotion.

4. Manufacture of fruit juice

Health enhancing fruit juice was prepared by adding 1 g of the extract of the present invention to 1,000 ml of apple or grape juice.

12: cooler 14: heat exchanger
16: pump 17: container

Claims (5)

delete 10 to 20 wt% of silkworm, 5 to 10 wt% of Puerariae radix, 5 to 10 wt% of raw persimmon, 5 to 10 wt% of Omija, 5 to 10 wt% of licorice, 20 to 50 wt% Drying the herbal medicine at 40 to 60 DEG C and cutting the dried herbal medicine to 0.1 to 0.2 cm;
The pulverized herbal medicines are charged into a supercritical extraction concentrator using a CO 2 or N 2 carrier gas, and the carrier gas circulates in a closed circuit space at a temperature of 56 to 60 ° C. and a gas volume of 8 to 19 m 3 / h. The vapor pressure of the concentrate located in the heat exchanger 14 is lowered and the carrier containing the solvent is transferred to the cooler 12 and the solvent is condensed and collected in the vessel 17, 16) to extract and concentrate the oriental medicinal herb;
Wherein the concentrated extract is charged into a bottle or a pack and then sterilized and packaged at 40 to 90 ° C to prepare a herbal tea extract composition 3. The method of claim 2,
Wherein the extract is concentrated in a high concentration of syrup or jelly in the step of extracting and concentrating the supernatant, 50 to 80% by weight of a herbal tea extract composition prepared by the method of claim 2 or 3,
And 20 to 50% by weight of a mixture for carbonated drinks obtained by mixing 5 to 20 wt% of sugar, 0.05 to 0.3 wt% of citric acid, 0.005 to 0.02 wt% of caramel, 0.1 to 1 wt% of vitamin C and 79 to 94 wt% of purified water After making the syrup,
Sterilizing the syrup at 85 to 98 캜 for 20 to 80 seconds, mixing the mixture with cooling water at a weight ratio of 1: 3.5 to 4.5, and then injecting carbonic acid gas. 50 to 80% by weight of a herbal tea extract composition prepared by the method of claim 2 or 3,
20 to 50% by weight of a mixture for health drinks mixed with 0.3 to 6.5% by weight of liquid fructose, 1 to 10% by weight of oligosaccharide, 1 to 10% by weight of sugar, 0.3 to 6.5% by weight of salt, and 68 to 87% ≪ / RTI > and then sterilized in an instant.

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