KR20190053108A - A composition comprising the complex extract for antiobesity of men - Google Patents

Abstract

The present invention relates to an anti-obesity composition containing a complex extract. More particularly, when taking the complex extract of a Pueraria montana var. lobate root, a Hovenia dulcis stem, an Eleutherococcus senticosus stem, a Schisandra chinensis fruit, a Lycium chinense fruit, and a Zizyphus jujube fruit, it is possible to exhibit an effect of significantly decreasing a body fat amount and an abdominal fat rat and an effect of inhibiting accumulation of visceral fat and subcutaneous fat. Therefore, the complex extract of the present invention can be usefully used as a composition or a functional material for preventing or treating obesity.

Description

TECHNICAL FIELD The present invention relates to a composition for male antiobesity containing a complex extract,

The present invention relates to an anti-obesity composition containing a complex extract, and more particularly, to an anti-obesity composition containing as an active ingredient, a complex extract of Liliaceae, Hovenia dulcis, Ogaki, Omija, Gugija, Sansui oil and Jujube.

Men who work at work often eat breakfast, and dinner often consumes considerably high calorie food for entertaining, gathering, and eating, and consumes more than 50% of the calories consumed per day for dinner. Thus, in the morning, energy is consumed by the lack of energy in the morning, and in the evening, fat accumulation due to excess energy is continued, resulting in reduced muscle mass and lower body energy consumption, resulting in obesity. According to the 'National Health and Nutrition Survey' and 'Online Survey on Youth Health Behavior' in 2016, the prevalence of obesity above the Korean BMI of 25 kg / m ² was 42.3% for men over 19 years old, The prevalence of obesity in males over 30 years of age was 4.3%, which was 1.5% increased over a year.

In particular, among obesity, there are people who are normal in weight but metabolically obese, and their common characteristic is abdominal obesity in which the stomach comes out. That is, even if the body weight is normal, the risk of diseases such as hypertension, diabetes, and dyslipidemia such as obesity is increased when the stomach comes out. Abdominal obesity usually occurs in people with insufficient activity and often causes abdominal obesity due to increased visceral fat mass. In addition, patients with abdominal obesity are often low in muscle mass, so it is important to maintain muscle mass in parallel with exercise.

On the other hand, in today 's complicated modern society, people who are exposed to environmental pollution due to deterioration of living environment, mental stress and lack of activity are increasingly interested in health promotion. Although exercise is the most effective and economical way to prevent adult diseases or aging, modern people who are unable to manage their health due to busy daily life and fatigue are interested in taking various functional foods as an alternative exercise. However, the abuse of functional foods, temporarily excessive exercise, is associated with the risk of adverse health effects. Accordingly, there has recently been a need for the development of functional adjuvants using natural products that are safe, such as plant extracts.

Accordingly, the present inventors have made efforts to develop a material capable of preventing obesity, especially abdominal obesity, by preventing side effects due to indiscreet diet and excessive exercise therapy, and as a result, it has been found that the above- , Corn oil, and jujube fruit juice, the effect of reducing body fat mass and abdominal fat ratio and the accumulation of visceral fat and subcutaneous fat were observed. The present inventors have completed the present invention by disclosing that the complex extract of the present invention can be used as a composition for preventing or treating obesity or as a functional material.

Korean Patent Publication No. 2015-0063183

Lee, Seung - Hyun, and Young - Chae Cho. 2011. Assessment of obesity by BMI and body fat percentage and correlation with risk factors for coronary artery disease. Journal of the Korean Academy of Industrial Science and Technology 12: 746-752. Lee Seung - gyo, Won Hyang Rye, and Jeong Eun - hee. Life Cycle Nutrition p.220 (2013) Life Science. Lee, Yeon - Sook, Jae - Ok Koo, Hyun - Sook Lim, Younghee Kang, and Kwon Jong - Sook. Easy to understand human physiology p.37 (2016) Powerbook.

It is an object of the present invention to provide a pharmaceutical composition for the prevention or treatment of obesity, which comprises as an active ingredient a complex extract of Lilac, Hovenia dulcis, Ogapi, Omija, Gugija, Corn oil and Jujube.

Another object of the present invention is to provide a health functional food for preventing or ameliorating obesity, which comprises, as an active ingredient, a combined extract of mulberry, hinoki, ogapi, omija, gugija, corn oil and jujube.

In order to accomplish the object of the present invention, the present invention provides a pharmaceutical composition for preventing or treating obesity, which comprises, as an active ingredient, a combined extract of mulberry, hinoki, ogapi, omija, gugija, corn oil and jujube.

The present invention also provides a health functional food for preventing or ameliorating obesity, which contains, as an active ingredient, a combined extract of mulberry, hinoki, ogapi, omija, gugija, corn oil and jujube.

The present invention relates to a method of preparing a hydrothermal composite extract by blending chestnut root, hornbush stem, ogassi stem, omiza berries, gingerbread fruit, cornstarch fruit and jujube fruit. When the hydrothermal extract is ingested, the body fat mass and abdominal fat ratio It is possible to use the complex extract of the present invention as an effective ingredient of a composition for preventing or treating obesity or a functional material.

FIG. 1 is a view showing the fat cell size of each experimental animal group prepared in one embodiment of the present invention. FIG.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for the prevention or treatment of obesity, which comprises, as an active ingredient, a combined extract of Lilac, Hovenia dulcis, Ogapi, Omija, Gugija, Corn oil and Jujube.

The combined extracts of the active ingredients of the present invention, such as 칡, hinoki, ogapi, omija, gugija, corn oil and jujube, are preferably prepared by a method including, but not limited to, the following steps:

(1) blending oak, hinoki, ogapi, omija, gugija, corn oil and jujube, adding water containing purified water to extract;

2) filtering the extract of step 1);

3) subjecting the filtrate of step 2) to reduced concentration; And

4) drying the concentrate of step 3).

In the production method of the present invention, in step (1) above, cultivated or commercially available flour, hinoki, ogapi, omija, gugija, corn oil and jujube can be used without limitation. In addition, at least one region selected from the group consisting of leaves, stem, root, and fruit thereof can be used as the above-mentioned tree, hinoki tree, ogapi, omija, gugija, corn oil and jujube, and more specifically, Ogassi stem, Gugija fruit, Cornus fruit and Jujube fruit can be used.

In the step 1), 20 to 40 parts by weight of starch, 15 to 35 parts by weight of hinoki, 15 to 35 parts by weight of omega, 0.5 to 15 parts by weight of omega, 0.5 to 15 parts by weight of starch, 0.5 to 15 parts by weight of jujuba, and 0.5 to 15 parts by weight of jujube are contained in an amount of 25 to 35 parts by weight, 20 to 30 parts by weight of hinoki, 20 to 30 parts by weight of omega, 1 to 10 parts by weight of omega, More preferably from 1 to 10 parts by weight, from 1 to 10 parts by weight, from about 1 to about 10 parts by weight, from about 1 to about 10 parts by weight, and from about 1 to about 10 parts by weight of jujube, , 3 to 7 parts by weight of omega, 3 to 7 parts by weight of omega, 3 to 7 parts by weight of ginger, 3 to 7 parts by weight of corn oil and 3 to 7 parts by weight of jujubes are more preferable, 30 parts by weight, 25 parts by weight of hinoki, 25 Parts, 5 parts by weight of Schisandra chinensis, 5 parts by weight of a Lycium chinense, 5 parts by weight of Corni fructus, and one is most preferred that the formulation 5 parts by weight of jujube, and the like.

In the production method of the present invention, it is preferable to use water or alcohol as the extraction solvent in the step 1), and more preferably to use water. As the alcohol, C ₁ to C ₄ lower alcohols are preferably used, and as the lower alcohol, ethanol or methanol is preferably used. As the extraction method, it is preferable to use shaking extraction, Soxhelt extraction or reflux extraction, but not limited thereto. The extraction solvent is preferably added by 1 to 10 times the weight of the mixture, more preferably 2 to 3 times. The extraction temperature is preferably 90 to 130 ° C, more preferably 95 to 125 ° C, but is not limited thereto. Further, the extraction time is preferably 1 to 48 hours, more preferably 2 to 10 hours, even more preferably 2 to 8 hours, but is not limited thereto.

In the production method of the present invention, it is preferable to use a vacuum decompressing concentrator or a vacuum rotary evaporator for the decompression concentration in step 3), but the present invention is not limited thereto. The drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but is not limited thereto.

In the production method of the present invention, step 3) and step 4) may be omitted depending on the use of the complex extract of the present invention.

In the present invention, the obesity may be abdominal obesity, and the abdominal obesity may be subcutaneous abdominal obesity, visceral fat type abdominal obesity or abdominal muscle type abdominal obesity, and more specifically, subcutaneous fat type abdominal obesity or visceral fat type Abdominal obesity can be.

The term " obesity " means an excess of fatty tissue in the body. Obesity is defined as the body mass index (BMI), which is the body mass index (BMI) divided by the square of height (m) More specifically, in Lee and Cho, "normal" when the BMI is less than 25, less than 20% of the body fat, "appearance-like" obesity, which is apparently fat when the BMI is 25 or more and the body fat percentage is less than 20% 25 and the percentage of body fat is over 20%. Such obesity increases the likelihood of diabetes and hyperlipemia, increases the risk of sexual dysfunction, arthritis, cholelithiasis and cardiovascular disease.

In addition, the term " abdominal obesity " refers to a case where fat is excessively accumulated in the abdomen of the abdomen, unlike obesity due to subcutaneous fat. The fat in the abdomen is nearer to the liver than the fat distributed elsewhere, so it is likely to have systemic effects. Abdominal obesity can also be subcutaneous fat, visceral fat or abdominal muscle type depending on its shape. The subcutaneous fat-type abdominal obesity is a type of abdominal subcutaneous fat, which is mostly present in postnatal or postmenopausal women and can be treated by ultrasonography or body weight reduction. The abdominal muscle-separated abdominal obesity is a type in which the abdomen protrudes even if there is no fat, and can be easily treated by endoscopic surgery. The visceral fat type abdominal obesity is a type of obesity which is very dangerous to health due to a lot of fat inside the abdomen, and liposuction or abdominal exercise, which is a common method of treating obesity, does not help.

In a specific example of the present invention, the inventors of the present invention obtained a hydrothermal composite extract of Lycoris spp., Hovenia dulcis, Opuntia ficus, Schizandra chinensis, Fagaceae fruit, Sansui oil and Jujube fruit, After 8 weeks of ingestion, body composition changes were measured. As a result, it was confirmed that not only the body fat amount but also the abdominal fat ratio were significantly decreased after the ingestion of the hot water complex extract.

In addition, the present inventors measured the body composition change after oral administration of the hydrothermal extract to an experimental animal for 8 weeks. As a result, the inventors of the present invention found that the extract of the present invention was orally administered to the high- Decreased body weight gain, and inhibited accumulation of visceral fat and subcutaneous fat.

Therefore, the inventors of the present invention have confirmed that the compound extract of the present invention has a significant effect of reducing the fat mass and the abdominal fat percentage, and inhibiting the accumulation of visceral fat and subcutaneous fat, And can be effectively used as an active ingredient of a pharmaceutical composition for preventing or treating abdominal obesity.

The pharmaceutical compositions according to the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the production of pharmaceutical compositions.

The pharmaceutical composition according to the present invention can be administered orally or parenterally, and can be administered orally or parenterally when an external or intraperitoneal injection, rectal injection, subcutaneous injection, intravenous injection, intramuscular injection, But is not limited thereto.

The pharmaceutical composition according to the present invention may be formulated in the form of oral, granule, tablet, capsule, suspension, emulsion, syrup, aerosol or the like oral preparation, external preparation, suppository and sterilized injection solution according to a conventional method Can be used. Examples of carriers, excipients and diluents that can be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose ), Lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the pharmaceutical composition according to the present invention varies depending on the condition and the weight of the patient, the degree of the disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art. However, for the desired effect, the composition is preferably administered at a dose of 0.00001 to 1 g / kg, preferably 0.001 to 200 mg / kg, per day, but is not limited thereto. The above administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way.

The present invention also provides a health functional food for preventing or ameliorating obesity, which contains, as an active ingredient, a combined extract of mulberry, hinoki, ogapi, omija, gugija, corn oil and jujube.

In the present invention, the complex extract is preferably extracted with water, a C ₁ to C ₄ lower alcohol, or a mixed solvent thereof. More specifically, the complex extract is selected from the group consisting of water, methanol, ethanol, Or more, and more specifically, it is preferably extracted with water, but is not limited thereto.

Wherein the complex extract comprises 20 to 40 parts by weight of hinoki, 15 to 35 parts by weight of hinoki, 15 to 35 parts by weight of omega, 0.5 to 15 parts by weight of omega, 0.5 to 15 parts by weight of corn oil, 0.5 to 15 parts by weight of corn oil, And 20 to 30 parts by weight of hinoki, 1 to 10 parts by weight of omega, 1 to 10 parts by weight of omega, More preferably 1 to 10 parts by weight of corn oil, 1 to 10 parts by weight of corn oil, and 1 to 10 parts by weight of jujube, 28 to 32 parts by weight of corn, 23 to 27 parts by weight of hovenwood, Still more preferably from 23 to 27 parts by weight of omega, from 3 to 7 parts by weight of omega, from 3 to 7 parts by weight of corn oil, from 3 to 7 parts by weight of corn oil and from 3 to 7 parts by weight of jujube, 25 parts by weight of Hovenia dulcis, 25 parts by weight of Ogaki , 5 parts by weight of omija, 5 parts by weight of corn oil, 5 parts by weight of corn oil, and 5 parts by weight of jujube, but is not limited thereto.

In addition, at least one region selected from the group consisting of leaves, stem, root, and fruit thereof can be used as the above-mentioned tree, hinoki tree, ogapi, omija, gugija, corn oil and jujube, and more specifically, Ogassi stem, Gugija fruit, Cornus fruit and Jujube fruit can be used.

The obesity may be abdominal obesity, and the abdominal obesity may be subcutaneous fat abdominal obesity, visceral fat abdominal obesity or abdominal muscle abdominal obesity obesity, more specifically subcutaneous fat abdominal obesity or visceral fat abdominal obesity have.

INDUSTRIAL APPLICABILITY Since the compound extract of the present invention has an effect of reducing abdominal fat percentage as well as body fat content in the body and inhibiting accumulation of visceral fat and subcutaneous fat, it is useful as an active ingredient of obesity, especially health functional food for prevention or improvement of abdominal obesity Lt; / RTI >

The health functional food of the present invention can be used as it is or in combination with other food or food ingredients, and can be suitably used according to conventional methods.

There is no particular limitation on the kind of the food. Examples of the food include dairy products including drinks, meat, sausage, bread, biscuits, rice cakes, chocolates, candies, snacks, confectionery, pizza, ramen noodles, other noodles, gums, ice cream, various soups, And a combination thereof, all of which include health foods in a conventional sense.

The mixing amount of the complex extract according to the present invention can be appropriately determined according to its use purpose (for prevention or improvement). Generally, the amount of the complex extract in the health food may be from 0.01 to 15% by weight of the total food. However, in the case of long-term intake intended for health and hygiene purposes or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

The health functional beverage composition of the present invention is not particularly limited to the other ingredients except that the complex extract is contained as an essential ingredient at the indicated ratio and may contain various flavors or natural carbohydrates as an additional ingredient have. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above .

In addition to the above-mentioned foods, the food of the present invention may contain flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the complex extract of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The ratio of such additives is not critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the combined extract of the present invention.

Hereinafter, the present invention will be described in detail with reference to Examples, Experimental Examples and Production Examples.

However, the following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples, Experimental Examples and Preparation Examples.

< Example  1> 칡, Hinoki , An extract of Ogaki, Omija, Gugija, corn oil and jujube

(1.65 kg), 25 weight% (1.375 kg) of Hovenian trunks, 25 weight% (1.375 kg) of Ogaji stem and 1.3 weight kg of Ojina berries in a high-speed vacuum low-temperature extractor (COSMOS-660, Kyungseo machine, Incheon, Korea) 60 L of purified water was added to 5 wt% (0.275 kg), 5 wt% (0.275 kg), 5 wt% (0.275 kg) and 5 wt% (0.275 kg) of jujube . After the extraction, the spout pouch automatic packing machine (SPS-A430, Sungil system corp, Daegu, Korea) was packed and used in the experiment.

< Comparative Example  1> Preparation of Ogaki Extract

5.5 kg of the cuttlefish stem and 60 L of purified water were added to a high-speed vacuum low-temperature extractor (COSMOS-660, Kyungseo machine, Incheon, Korea) and extracted by the same method as described in <Example 1>.

< Experimental Example  1> Identification of the effect of compound extract on obesity

In order to confirm the effect of improving the obesity and the effect of increasing the muscle mass of the compound extract prepared in Example 1, 40 to 50 male subjects were divided into two groups: pre-ingestion and post-ingestion of the complex extract prepared in Example 1 Composition changes were measured.

Specifically, the study was conducted on twelve men in their 40s and 50s who were in good health. Body weight, body fat, fat mass, abdominal fat percentage, BMI (body mass index: body weight (kg)) were measured for height (m) using InBody 720 (InBody Co., Ltd., Cheonan, Korea) It was measured in the body composition of factors, including the value (kg / m ²⁾⁾ divided by the square, the measurement time in consideration of the physical condition of the subject before meals in the morning (10: measured on a 00): 00-12. Among the above-mentioned body composition factors, BMI is the most commonly used obesity index because it has a high correlation with body fat percentage among the body composition index. The Obesity Society of Korea presents obesity evaluation criteria for Koreans and diagnoses obesity if BMI is over 25 kg / m ² . However, since BMI is measured only by height and weight, there is a disadvantage that BMI may be high for people who have increased weight due to increased muscle mass. For more accurate analysis, body water, fat mass, fat mass, . Here, it is known that the water content is inversely proportional to the body water content and the body fat, and that the moisture content depends on the fat content. In addition, the fat-free amount means a value excluding the fat amount from the body weight.

Then, the subjects who had completed the measurement were allowed to consume 100 mL of the combined extract prepared in Example 1 for 8 weeks in the morning and evening, and then the constituent factors of the body were measured in the same manner as described above And body composition changes before ingestion. On the other hand, all subjects participating in the trial were given the same diet during the test, but were prohibited from taking or injecting drugs.

The mean and standard deviation of each constituent were calculated using SPSS v12.0 (SPSS Inc., Chicago, IL, USA) statistical program. The pre- and post-intake t-test was p <0.05 p <0.01 and p <0.001, respectively.

As a result, as shown in Table 1, when the complex extract of the present invention was consumed for 8 weeks, it was confirmed that the body fat amount was significantly reduced from 18.43 ± 5.27 kg to 16.40 ± 4.35 kg. In particular, it was confirmed that the abdominal fat ratio was significantly decreased from 0.88 ± 0.03 to 0.86 ± 0.05 before the intake, even though the subjects did not exercise regularly while taking the complex extract of the present invention. Also, it was confirmed that the body water content increased significantly from 40.15 ± 4.07 L to 41.19 ± 0.66 L due to the decrease of body fat content. In addition, it was confirmed that the fat content was 54.65 ± 5.63 kg before consumption of the compound extract of the present invention, but it was 56.05 ± 6.13 kg after ingestion. Therefore, it was confirmed that the combined extract of the present invention has the effect of improving obesity, especially abdominal obesity.

Before ingestion After 8 weeks of ingestion Variation t-value p-value Age (years) 51.82 + - 5.00 Height (cm) 169.49 + - 4.93 169.76 + - 4.97 - -1.703 0.119 Weight (kg) 73.08 ± 10.15 72.45 + - 8.53 - 0.909 0.385 BMI (kg / m 2) 25.47 ± 3.08 25.11 + - 2.48 - 1.322 0.216 Body water (L) 40.15 + - 4.07 41.19 + - 4.44 1.04 + - 0.66 -5.179 ^*** 0.000 Fat Weight (kg) 54.65 ± 5.63 56.05 ± 6.13 1.39 ± 0.90 -5.123 ^*** 0.000 Fat mass (kg) 18.43 + - 5.27 16.40 + - 4.35 -2.03 ± 2.65 2.542 ^* 0.029 Abdominal fat rate 0.88 + 0.03 0.86 ± 0.05 -0.03 0.04 2.507 ^* 0.031

^* p < 0.05, ^** p < 0.01, ^*** p < 0.001

< Experimental Example  2> Confirmatory effect of combined extract on visceral fat type and subcutaneous fat type abdominal obesity

<2-1> Production of experimental animals

Twenty-four C57BL / 6J mice (BioLink, Chungbuk, Korea) were purchased and stabilized at 24 ± 2 ° C for 12 weeks. Then, 6 rats were randomly divided into the experimental group of [Table 2]. Distilled water, the complex extract prepared in Example 1 or the extract of Ogaki prepared in Comparative Example 1 was orally administered to each experimental group for 8 weeks once a day. In addition, the normal group was fed normal diet, and the remaining experimental group was fed a high fat diet (Research D12451) containing 45% fat for 8 weeks.

Experimental group The administered sample Diet Normal group One 8-week distilled water Normal diet It's a highland system. One 8-week distilled water 45% highland system High-fat diet + acupuncture extract-treated group Oral extract of 18 mg / kg / day for 8 weeks 45% highland system High fat diet + combination extract group 8 week compound extract 18 mg / g / day Oral administration 45% highland system

<2-2> Confirmation of weight loss by compound extract

The body weight of each of the experimental groups of Experimental Example < 2-1 > was measured before oral administration of the sample and after 8 weeks of oral administration.

As a result, as shown in Table 3, it was confirmed that the weight gain of the high-fat diet group was the highest at 9.09 ± 0.21 g. On the other hand, it was found that the weight gain was lower in the high - fat diet + high fat diet + high fat diet than the high fat diet. In addition, the dietary intakes of the high - fat diet group and the high fat diet group were similar, and it was confirmed that the dietary intake had no effect on the anti - obesity effect of the combined extracts.

Experimental group Initial Weight
(g) Final weight
(g) Weight gain
(g) Dietary intake
(g / day / mice) Normal group 20.36 ± 1.03 26.82 ± 1.73 5.81 ± 0.38 2.68 ± 0.01 It's a highland system. 20.37 ± 0.91 29.46 + - 0.74 9.09 0.21 2.55 + 0.07 High-fat diet + acupuncture extract-treated group 20.42 ± 0.92 27.36 ± 1.09 7.22 ± 1.91 2.31 + 0.14 High fat diet + combination extract group 20.39 ± 0.88 27.95 + - 0.57 7.88 ± 0.16 2.56 + 0.07

<2-3> Confirmation of visceral fat reduction by compound extract

In each of the experimental groups of Experimental Example <2-1>, changes of visceral fat were observed after oral administration for 8 weeks.

Specifically, the samples were orally administered to each of the experimental groups of Experimental Example <2-1> for 8 weeks and then fasted for 12 hours before the sacrifice. On the last day of the experiment, ether was inhaled and blood was collected under anesthesia. In order to investigate the weight of adipose tissue, visceral fat was extracted, washed with physiological saline, and then dehydrated with filter paper and weighed.

As a result, as shown in Table 4, it was confirmed that visceral fat of the high fat diet group was significantly increased as compared with the normal group. On the other hand, visceral fat of the high - fat diet + group was 0.3199 ± 0.0457 g, which was significantly lower than that of high fat diet group. On the other hand, the visceral fat of the high - fat diet + extract group was significantly higher than that of high fat diet.

Experimental group Visceral fat (g) Normal group 0.2668 ± 0.1217 It's a highland system. 0.4615 + 0.0394 High-fat diet + acupuncture extract-treated group 0.6305 + 0.0486 High fat diet + combination extract group 0.3199 + 0.0457

<2-4> Effect of Complex Extract on Fat Cell Size Reduction

Each of the experimental groups of Experimental Example <2-1> was orally administered with a sample for 8 weeks, and then the size change of the adipocytes was confirmed.

Specifically, the test group was orally administered to each of the test groups of Experimental Example <2-1> for 8 weeks, and then the mouse visceral fat tissue of each test group was carefully removed and washed with PBS. For histological analysis, the cells were fixed in 10% formalin solution and embedded in paraffin. The sections were cut to a thickness of 4 μm and cells were stained with hematoxylin-eosin (HE). Then, adipocytes were observed with a slide scanner (Aperio AT2, Leica bio systems Ltd., Germany).

As a result, as shown in FIG. 1, it was confirmed that the fat cell size of the high fat diet method + complex fat extract administration group was lower than that of the high fat diet method and high fat diet method + oxidant extract administration group.

As a result of the above results, it was confirmed that the combined extract of the present invention inhibits accumulation of visceral fat and subcutaneous fat, thereby more effectively improving visceral fat and subcutaneous fat abdominal obesity.

The preparation examples for the composition of the present invention are illustrated below.

< Manufacturing example  1 > The complex extract of the present invention Preparation of pharmaceutical preparations containing active ingredients

<1-1> Sanje  Produce

10 mg of the compound extract of the present invention

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

<1-2> Preparation of tablets

0.1 mg of the complex extract of the present invention

Corn starch 100 mg

100 mg of milk

Magnesium stearate 2 mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

&Lt; 1-3 > Preparation of capsules

0.1 mg of the complex extract of the present invention

Corn starch 100 mg

100 mg of milk

Magnesium stearate 2 mg

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

&Lt; 1-4 >

1 mg of the compound extract of the present invention

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

<1-5> Preparation of granules

0.15 mg of the complex extract of the present invention

Soybean extract 50 mg

Glucose 200 mg

Starch 600 mg

After mixing the above components, 100 mg of 30% ethanol was added and dried at 60 캜 to form granules, which were then filled in a capsule.

< Manufacturing example  2 > Production of a health food containing the compound extract of the present invention as an active ingredient

<2-1> Production of flour food

0.5 to 5.0 parts by weight of the combined extract of the present invention was added to wheat flour and the mixture was used to prepare bread, cake, cookies, crackers and noodles.

<2-2> soup  And gravies

0.1 to 5.0 parts by weight of the combined extract of the present invention was added to the soup and the juice to prepare a health improvement meat product, noodle soup and juice.

<2-3> Ground Beef  Produce

10 parts by weight of the combined extract of the present invention was added to ground beef to prepare a ground beef for health promotion.

<2-4> Manufacture of dairy products

5 to 10 parts by weight of the combined extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<2-5> Solar  Produce

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The combined extract of the present invention was concentrated under reduced pressure in a vacuum concentrator, dried by spraying and dried in a hot air drier, and pulverized to a size of 60 mesh with a pulverizer to obtain a dry powder.

The grains, seeds, and complex extract of the present invention prepared above were blended in the following proportions.

(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

The combined extract of the present invention (3 parts by weight)

(0.5 part by weight),

(0.5 parts by weight)

< Manufacturing example  3> A pharmaceutical composition containing the complex extract of the present invention as an active ingredient Health drink  Produce

<3-1> Health drink  Produce

100 ml of the combined extract of the present invention and a sub ingredient such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75% And then packed in small containers such as glass bottles and plastic bottles.

<3-2> Preparation of vegetable juice

100 ml of the complex extract of the present invention was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice.

<3-3> Production of fruit juice

100 ml of the complex extract of the present invention was added to 1,000 ml of apple or grape juice to prepare fruit juice.

Claims (8)

A pharmaceutical composition for the prevention or treatment of obesity, which comprises, as an active ingredient, a complex extract of Liliaceae, Hovenia dulcis, Ogassi, Omija, Gugija, Corn oil and Jujube.
The pharmaceutical composition for preventing or treating obesity according to claim 1, wherein the complex extract is extracted with water, a C ₁ to C ₄ lower alcohol or a mixed solvent thereof.
[Claim 2] The composition according to claim 1, wherein the complex extract comprises 20 to 40 parts by weight of hinoki, 15 to 35 parts by weight of hinoki, 15 to 35 parts by weight of omega, 0.5 to 15 parts by weight of omega, 0.5 to 15 parts by weight 0.5 to 15 parts by weight of marine oil, and 0.5 to 15 parts by weight of jujube.
The pharmaceutical composition for preventing or treating obesity according to claim 1, wherein the extract is selected from the group consisting of leaf, stem, root, and fruit, characterized in that the extract is selected from the group consisting of leaves, hornbills, oggies, omija, Composition.
The pharmaceutical composition for preventing or treating obesity according to claim 1, wherein the obesity is abdominal obesity.
[Claim 7] The pharmaceutical composition according to claim 5, wherein the abdominal obesity is selected from the group consisting of subcutaneous fat-type abdominal obesity, visceral fat type abdominal obesity, and abdominal muscle type abdominal obesity.
A health functional food for prevention or improvement of obesity which contains, as an active ingredient, a combined extract of green tea, hinoki tree, ogapi, omija, gugija, corn oil and jujube.
[Claim 7] The composition according to claim 7, wherein the complex extract has 20 to 40 parts by weight of hinoki, 15 to 35 parts by weight of hinoki, 15 to 35 parts by weight of omega, 0.5 to 15 parts by weight of omega, 0.5 to 15 parts by weight 0.5 to 15 parts by weight of marine oil, and 0.5 to 15 parts by weight of jujube.

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