KR20160011846A - Anti-obesity composition containing bitter melon mixture hot water extract and manufacturing method thereof - Google Patents

Abstract

The present invention relates to an anti-obesity pharmaceutical composition comprising a hot water extract of Momordica charantia, Corni fructus, Schizandra chinensis Baillon. and Zizyphus jujuba Mill as an active ingredient. The hot water extract of Momordica charantia, Corni fructus, Schizandra chinensis Baillon. and Zizyphus jujuba Mill exhibits effects in reducing the body weight, reducing diet efficiency, reducing the weight of the liver and the epididymal fat, reducing the contents of serum lipid, reducing the contents of liver lipid, etc. Therefore, the composition comprising the extract can be efficiently used for preventing or treating obesity or obesity-related diseases.

Description

TECHNICAL FIELD The present invention relates to an anti-obesity composition containing bitter melon extract,

The present invention relates to a pharmaceutical composition having an anti-obesity effect, which comprises a hot-water extract of Yeoju, Sansui, Omija and Jujube as an active ingredient, a health functional food, a food and a method for producing the same.

Obesity is a complex syndrome in which body fat accumulates in the subcutaneous tissues or mesentery and gradually increases in weight when energy is consumed in excess of body energy consumption, and various causes such as genetic, nutritional, environmental and social factors are involved to be. According to the World Health Organization (WHO) report, currently over one billion people are overweight and over 300 million people are classified as obese people with a BMI greater than 30 kg / m ² . Obesity is not only known to be responsible for diabetes, hypertension, arteriosclerosis, hyperlipidemia, cardiovascular disease, obesity and various metabolic disorders such as fatty liver (Manson et al., New England J. Med., 333, pp677-685, (JAMA, 282, pp1523-1529, 1999), which in turn causes the abdomen to be compressed by adipose tissue induced by obesity, causing constipation and digestion Deficiencies, and gastrointestinal disorders, the World Health Organization (WHO) has warned that obesity is a disease that requires treatment. As obesity is recognized as a disease, it is necessary to develop materials that prevent or treat obesity effectively.

The main means for the treatment and improvement of obesity include diet, moderate exercise and drug administration, but the side effects of obesity treatment drugs are various.

Representative anti-obesity drugs approved by the US Food and Drug Administration (FDA) for long-term use include orlistat and sibutramine. Orlistat, which is marketed under various product names such as 'Xenical', 'Lipidon', and 'Oliet', monopolizes the market for obesity drugs, Of gastrointestinal side effects have been reported. In addition, sibutramine, which is marketed under the trade names Reductil and Meridia, increases side effects of serotonin and noradrenaline in the sympathetic nervous system, resulting in side effects such as headache, anorexia, insomnia, constipation . In addition to the anti-obesity drugs that have been used so widely, many of the products that have been developed have been banned due to serious side effects.

In recent years, oriental herbs and natural products have been attracting attention as a therapeutic agent for obesity in order to minimize various side effects. However, it is cited from the data based on the traditional Chinese medicine book, and there is absolutely lack of systematic and scientific evidence and clinical results about the efficacy of each material. Therefore, based on the folk remedies that have been used for a long time without side effects, we have searched for useful ingredients from traditional biological resources and developed more effective anti-obesity functional materials to improve the competitiveness of functional materials technology using traditional oriental herbal compositions, It is time to develop individual pharmacopoeial authentication techniques.

Korean Patent No. 1,074,914 discloses that Momordica charantia fermented product activates AMP activated protein kinase (AMPK) and inhibits ACC (Acetyl-CoA carboxylase), one of its targets, to inhibit lipogenesis and adipocyte differentiation, Can be used for the treatment or prevention of obesity with an obesity effect.

Korean Patent No. 1,144,197 discloses that Psoraleae Semen extract, Siegesbeckiae Herba extract and Corni Fructus extract have an angiogenic and obesity inhibitory effect and thus can be useful as an angiogenesis-related disease and a therapeutic agent for obesity .

Korean Patent Laid-Open Publication No. 2012-0112137 discloses that the fraction of Omija ethyl acetate or the UWW index C isolated therefrom inhibits the differentiation of adipocytes and has an excellent effect of inhibiting the accumulation of lipids, so that it is useful for preventing or treating obesity Is disclosed.

On the other hand, Momordica charantia is a vine plant of pak and it is called bitter melon in English name. It grows up to 1 m in length with a stem, and hoops up another thing with a tendril. The fruit is long, elliptical, narrow in both ends, with lumpy protrusions, and irregularly ripened in yellowish red, resulting in seeds shrunken in red flesh. The fruit is called Yeoju because it is similar to open space. Young fruit, red seed coat and seeds are used for medicinal purposes. Recently, it has been reported that it can be used for the treatment of diabetes (Miura T. et al., J. Nutr. Sci. Vitaminol., 47 (5), 340-344, 2001; Grover JK et al., J Ethnopharmacol., 76 (3), 233-238, 2001).

As a physiologically active substance of Yeoju, momordicin, charantin, and conjugated linoleic acid belonging to plant sterols are known. In addition, Yeoju contains 120 mg of 100 g of vitamin C, which is significantly higher than 80 mg of strawberry, 40 mg of cabbage, and 90 mg of lemon, and Vitamin C in Yeoju is contained in juicy pulp Therefore, even if heated, it is hardly broken.

Cornu Fructus is a fruit of the Cornus fructus , a deciduous tree in the Tertiary tree. It is an elliptical nucleus and it is green at first, but it ripens red from August to October. Seeds are long oval and have ridges. It has a sweet taste and a strong acidity. It separates the meat and seed, and the meat is used as a material for alcohol, tea, and Chinese medicine. The pulp contains the glycosides such as cornin, Morroniside, Loganin, tannin and saponin and organic acids such as grape juice, malic acid and tartaric acid. It also contains vitamin A and a large amount of sugar. Seeds include palmitic acid, oleic acid, linoleic acid, and the like. It is said that it has the efficacy such as strengthening of 阴 阴, 肾 精 and 肾气, and convergence according to "Dongbibogam" and "Flavor", and it is used as a medicine for headache, tinnitus, It is also used for folk remedies such as sweat and enuresis.

Schizandra chinensis Baillon ( Schizandra chinensis Baillon.) Is a deciduous broad-leaved vine, with alternate leaves, ovate or obovate, with sharp tips, sharp teeth, and slightly hairs on the back side. The flower is reddish white, bloomed in June-July, and it is migratory. Fruit is used for food, hangs in the shape of a spike at the end of the branch, and ripens red from August to September. The fruit is called Schizandrae fructus and Schizandrae fructus . 200 ~ 1,600 m above sea level. It is the main province of Jirisan, Jeonbuk and Gangwon provinces. It is wild throughout the country excluding Chungnam and Chungbuk, and is distributed geographically in Japan, Sakhalin, Manchuria and China. Fruits include Schizandrol, Schizandrin A, B, C, angeloylgomisin O, P, epigomisin, pregomoisin, deoxyschizandrin ) And the like. Omija has been used for herbal medicine raw materials for a long time and has medicinal properties such as astringency, nourishment, tonic, shinhae medicine, Haegok poison, thirst, convergence, Human stability due to ingestion has already been confirmed.

Jujube ( Zizyphus jujuba Mill var.) Is a broad-leaved arboreous tree belonging to the family Seagrass. Jujube is widely grown because it is easy to cultivate and has good shelf life. Jujube contains nutrients such as protein, fat and 36 various inorganic elements. Especially, nutrients such as vitamin C, vitamin B group, calcium, and phosphorus are contained as much as natural vitamin supplements. In addition, the moisture content of the jujube is 74%, the moisture content of red jujube is 60%, and the moisture content of dried jujube is 30%. The content of glucose and fructose, which are main components of jujube, is 28% and the content of protein is 1.5%. It has been reported that ethanol extract of jujube is administered to liver damaged rats and the recovery and protection effect of injured liver is good. In one room, jujube is used as a relaxant tonic, and ripe jujube is matured and dried. It is known that it eats when it eats, it lowers fever, it reduces constipation and stops coughing. In recent years, Cyclic AMP) is active in the jujube, the researchers report.

Accordingly, the inventors of the present invention have conducted research to develop a composition for treating obesity that does not exhibit side effects at low cost in a short time using water (purified water) without processing such as fermentation using the natural material as described above. As a result, The inventors of the present invention have completed the present invention based on the finding that the hot water extract of Yeoju, Sansui, Omija, and Jujube according to the manufacturing method exhibits weight loss and lipid improvement without toxicity such as liver damage.

Korean Patent No. 1,074,914 Korean Patent No. 1,144,197 Korea Patent No. 2012-0112137

Manson et al., New England J. Med., 333, pp. 677-685, 1995. Kopleman P. G., Nature, 404 pp635-643, 2000. Must et al., JAMA, 282, pp1523-1529, 1999. Miura T. et al., J. Nutr. Sci. Vitaminol., 47 (5), 340-344, 2001. Grover JK. et al., J. Ethnopharmacol., 76 (3), 233-238, 2001.

It is an object of the present invention to provide a pharmaceutical composition for an anti-obesity containing the hydrothermal extract as an active ingredient, a health functional food, a food and an anti-obesity And to provide a method for producing a pharmaceutical composition.

In order to attain the above object, the present invention confirms that the hot water extract of Yeoju, Sansui, Omija, and Jujube of a specific ingredient ratio exhibits activities such as weight loss, reduction of dietary efficiency, improvement of lipid, As an active ingredient, an anti-obesity pharmaceutical composition, a health functional food, and a food.

According to the present invention, the hot-water extract of Yeoju, Sansui, Omija, and Jujube does not exhibit toxicity such as liver damage, but also decreases weight, decreases dietary efficiency, decreases liver and epididymal fat weight, decreases serum lipid content, And thus can provide a pharmaceutical composition for an anti-obesity containing the mixed extract as an active ingredient, a health functional food, a food, and a method for producing the same.

FIG. 1 is a flow chart showing a method for producing hot water extract of Yeoju, Sansui, Omija, and Jujube according to the present invention.

The present invention relates to a pharmaceutical composition for anti-obesity comprising hot water extract of Yeoju, Sansui, Omija, and Jujube as an active ingredient.

In one embodiment of the present invention, the hot-water extract has a weight ratio of 100: 10 to 20: 5 to 15: 1 to 10, more specifically 100: 10 to 15: 5 to 10: 1, 5, more specifically from 100: 12 to 14: 8 to 10: 3 to 5 by weight. It was confirmed from the experimental results that the compound had the best anti-obesity activity in the blending ratio.

In one aspect of the present invention, the pharmaceutical composition for anti-obesity has at least one activity selected from the group consisting of weight loss, reduction of dietary efficiency, decrease of blood lipid content, and decrease of liver lipid content.

The pharmaceutical composition of the present invention may comprise a pharmaceutically acceptable carrier. The composition comprising a pharmaceutically acceptable carrier may be of various oral or parenteral formulations. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain one or more excipients such as starch, calcium carbonate, sucrose or lactose, gelatin, . In addition to simple excipients, lubricants such as magnesium stearate, talc, and the like may also be used. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The pharmaceutical composition may be in the form of tablets, pills, powders, granules, capsules, suspensions, solutions, emulsions, syrups, sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze- It can have one formulation.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount.

The term "pharmaceutically effective amount " as used herein means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will vary depending on the species and severity, age, sex, , The sensitivity to the drug, the time of administration, the route of administration and the rate of excretion, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts. 1 to 10 mg / kg, preferably 3 to 6 mg / kg per day.

The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents exhibiting an anti-obesity effect or lipid-improving effect, and may be administered sequentially or simultaneously with conventional therapeutic agents. And can be administered singly or multiply. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without adverse effect, and can be easily determined by those skilled in the art.

The term "individual " as used herein refers to all animals, including humans, who have already developed or are capable of developing a disease that can be prevented or treated through anti-obesity or lipid-improving activity, and a composition comprising the extract of the present invention The above diseases can be effectively prevented and treated.

The route of administration of the pharmaceutical composition may be administered through any conventional route so long as it can reach the target tissue. The composition of the present invention may be administered intraperitoneally, intravenously, intramuscularly, subcutaneously, intradermally, orally, intranasally, intrapulmonarily, or rectally, though it is not intended to be limited thereto. The composition may also be administered by any device capable of transferring the active agent to the target cell.

The present invention relates to an anti-obesity health functional food comprising hot-water extract of Yeoju, Sansui, Omija, and Jujube.

In one embodiment of the present invention, the hot-water extract has a weight ratio of 100: 10 to 20: 5 to 15: 1 to 10, more specifically 100: 10 to 15: 5 to 10: 1, 5, more specifically from 100: 12 to 14: 8 to 10: 3 to 5 by weight.

In one embodiment of the present invention, the anti-obesity health functional food may be one having at least one activity selected from the group consisting of weight loss, reduction of dietary efficiency, decrease of blood lipid content, and decrease of liver fat content.

The term "health functional food " in the present invention refers to a food prepared and processed in the form of tablets, capsules, powders, granules, liquids and rings by using raw materials and components having useful functions in the human body. The health functional food of the present invention can be prepared by a method commonly used in the art, and the health functional food of the present invention can be manufactured by the method Unlike general medicines, there is an advantage that there are no side effects that may occur when a drug is taken for a long time by using a food as a raw material, and the portability In addition, the health functional food of the present invention can be ingested as an adjuvant for enhancing the effect of inhibiting angiogenesis.

The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). In general, the hot water extract of Yeoju, Sansui, Omija, and Jujube according to the present invention is added in an amount of 1 to 10% by weight, preferably 3 to 8% by weight of the raw material composition. However, in the case of long-term ingestion intended for health and hygiene purposes or for the purpose of controlling health, the amount can also be used in the above-mentioned range.

The health food of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary foods. The above-mentioned natural carbohydrates are sugar saccharides such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tau Martin and stevia extract, synthetic sweetening agents such as saccharine and aspartame, and the like can be used. The ratio of the natural carbohydrate is generally about 1 to 10 g, preferably about 1 to 5 g per 100 g of the composition of the present invention.

The food according to the present invention may include a hot-water extract of Yeoju, Sansui, Omija, and Jujube, but suitable food supplementary additives may be included.

The term "food-aid additive " in the present invention means a component which can be added to foods in a supplementary manner, and it can be appropriately selected and used by those skilled in the art as being added to produce health functional foods of the respective formulations. A coloring agent and a filler, a pectic acid and a salt thereof, an alginic acid and a salt thereof, an organic acid, a protective colloid thickener, a pH adjuster, a stabilizer, a stabilizer, and the like, which are various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, A preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, etc. However, the types of the food-aid additive of the present invention are not limited by the above examples.

The present invention relates to an anti-obesity food comprising a hot-water extract of Yeoju, Sansui, Omija, and Jujube.

In one embodiment of the present invention, the hot-water extract has a weight ratio of 100: 10 to 20: 5 to 15: 1 to 10, more specifically 100: 10 to 15: 5 to 10: 1, 5, more specifically from 100: 12 to 14: 8 to 10: 3 to 5 by weight.

In one aspect of the invention, the anti-obesity food may be one having at least one activity selected from the group consisting of weight loss, reduction of dietary efficiency, decrease of blood lipid content, and decrease of liver fat content.

There is no particular limitation on the kind of the food. Examples of the food to which the above substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, and includes foods in a conventional sense.

(A) adding and mixing purified water of 10 times or more the weight of the mixture of Yeoju, Sansui, Omija, and Jujube; And (b) subjecting the mixed solution to hot water extraction at 100 to 125 캜 for 3 hours or more.

In one embodiment of the present invention, in the step (a), the ratio of flax seed oil: flax seed: jujube is 100: 10 to 20: 5 to 15: 1 to 10, more specifically 100: 10 to 15: 5 to 10: 1 to 5, more specifically from 100: 12 to 14: 8 to 10: 3 to 5.

In one embodiment of the present invention, in step (a), the mixture of Yeast, Corn oil, Omiza, and Jujube may be in the form of a dry powder of each fruit.

Hereinafter, the present invention will be described in more detail with reference to the following Examples, Experimental Examples and Preparation Examples. It should be understood, however, that the same is by way of illustration and example only and is not intended to limit the scope of the present invention.

[Example]

60 L of purified water was mixed with 80% (solid content 12%), 10% (solid content 1.5%), 7% (solid content 1.0%) and jujube fruit 3% (solid content 0.4% (COSMO-660, Kyungseo machine, Incheon, Korea) at 103 ° C for 5 hours. After the extraction, the spout pouch automatic packaging machine (SPS-A430, Sungil system corp, Daegu, Korea) was packed and used for the experiment.

[Experimental Example]

Experimental Example 1:

- Extraction and material mixing

The water was extracted 10 times by weight with respect to each of mulberry juice, mulberry juice, omija juice, and jujube juice. The juice was then extracted with hot water at 110 ° C for 4 hours, and then filtered through a filter paper to obtain Yeast extract, Corn oil extract, Omiza extract and Jujube extract.

The mixed extracts prepared by various blends and ratios were analyzed by sensory evaluation, and then the following three mixed extracts with favorable taste were used for the experiment.

The first mixed extract was prepared by mixing 80% by weight of Yeast extract, 10% by weight of Sansui oil extract and 10% by weight of distilled water, and the second mixed extract contained 80% by weight of Yeast extract, 10% by weight of Sansui oil extract, 10% And the third mixed extract was prepared by mixing 80% by weight of Yeast Extract, 10% by weight of Cornus syrup extract, 7% by weight of Omiza extract and 3% by weight of Jujube Extract.

Each sample was filtered through a microfiltration filter and filter paper (Whatman, No. 2) to remove solids, then concentrated to 50 mL (N-1200, EYELA, Tokyo, Japan) and diluted to 2 mg / mL with distilled water Respectively.

- DPPH radical scavenging performance

1.2 mL of 1.5 × 10 ^-4 M 1,1-diphenyl-2-picryl hydrazyl (DPPH) was added to 1.8 mL of the sample, and the mixture was reacted at 37 ° C. for 30 minutes. Then, the absorbance at 517 nm Respectively. The radical scavenging activity was expressed by the absorbance reduction rate of the sample and the control. The control group used distilled water instead of the sample. The radical scavenging activity was calculated by the following equation.

Radical scavenging activity (%) = (1-absorbance of sample added / absorbance of control) × 100

(DPPH) radical scavenging activity of mulberry juice extract, citrus jujube extract, omija extract and jujube extract were 5.5, 67.1, 42.6 and 86.3%, respectively. The antioxidative effect of the extracts of L. japonica extract was the lowest and the antioxidative effect of jujube extract was the highest.

The radical scavenging activity of the first mixed extract was 83.9% and the radical scavenging activity of the second mixed extract was 89.6%. The radical scavenging activity of the third mixed extract was 92.5%. From the above experiment, the antioxidative effect of the third mixed extract was most excellent among the three mixed extracts. In addition, the antioxidative effect of the mixed extracts was higher than that of Yeoju extract, Oryza sativa, Omija extract, and Jujube extract. In particular, the third mixed extract had the highest antioxidative effect compared to the extracts of the respective alone and the first and second mixed extracts (Table 1), even though the extract contained 80 wt% of the lowest extract. It is presumed that mixing four kinds of extract greatly increases antioxidant activity by synergy effect.

○ Animals and diets for animal diet experiment

C57BL / 6J mice, 12 weeks old, were purchased from BioLink (Chungbuk province, Korea) and used. The animals were fed with water and diet freely. The experimental conditions were 20 ± 2 ℃, 50 ± 5 ℃ and 12 hours. This animal experiment was conducted with approval from the Experimental Animals Ethics Committee (Sangji University) (approval number: 2013-2). After a week of regular diet, 8 rats were divided into 3 groups according to the nude method. As shown in Table 3, physiological saline was administered to 50% beef tallow high fat diet, and 50 μl of beef tallow high fat diet was administered to the control group at the same time every day for 8 weeks in a volume of 100 μL and 250 μL, . The composition of 50% beef tallow is shown in Table 2.

○ Statistical processing of experimental results

The data obtained from the experiments were analyzed using a PC package (SPSS 21.0, statistical package for the social science, Chicago, IL, USA). The results were expressed as mean ± SD. The mean difference between the experimental groups was analyzed by one-way ANOVA followed by Duncan's multiple range test. Respectively.

Experimental Example 2. Weight gain, dietary intake and diet efficiency

Dietary intake and body weight were measured once a week (10 to 11 am) during the experiment. The body weight gain was measured by subtracting body weight before the start of the experiment and the weight gain during the rearing period was divided by the dietary intake of the same period to calculate the food efficiency ratio (FER) of each experimental group.

The body weight gain, the dietary intake, and the diet efficiency of the experimental rats after oral administration of 50 μl of the beef tallow diet at a dose of 100 μL and 250 μL for 8 weeks were shown in Table 4 . The final weight of experimental rats did not show any significant difference in each group, but the mixed extract of Yeoju was decreased by oral administration of 250 μL. The weight gain was 9.78 ± 0.33 g in the HFD group, 8.68 ± 0.21 g in the HFD BM100 group, and 7.09 ± 0.98 g in the HFD BM250 group, which were significantly decreased in the HFD BM250 group compared to the HFD group. Dietary intakes were not significantly different between groups. However, when dietary efficiency was compared, HFD BM250 group was 0.437 ± 0.061, which was significantly lower than HFD group (0.598 ± 0.020).

Experimental Example 3 Liver, spleen, and epididymal fat weight change

After 8 weeks of fasting, the animals were fasted for 12 hours and then lapped under ether anesthesia to remove liver, spleen, and epididymal fat. After removing blood and other substances with 0.9% NaCl solution, Were measured.

Table 5 shows the weight changes of liver, spleen and epididymal fat of the experimental rats after oral administration of 50% beef tallow high-fat diet mixed juice for 8 weeks. The liver weight of the HFD group was 1.153 ± 0.081 g, the HFD BM100 group was 1.136 ± 0.064 g, and the HFD BM250 group was 0.978 ± 0.036 g. The weight of epididymal fat was 1.043 ± 0.117 g in the HFD BM250 group, which was significantly lower than that in the HFD group (1.383 ± 0.248 g) and the HFD BM100 group (1.344 ± 0.187 g). The weight of the spleen was not significantly different among the groups.

Experimental Example 4: Lipid content of serum

The animals were fasted for 12 hours and then harvested under ether anesthesia. The animals were collected through the abdominal aorta and left at room temperature for 30 minutes. Serum was obtained by centrifugation at 3,000 rpm for 15 minutes and stored at -70 ° C. until analysis.

Plasma triglyceride, total cholesterol and high-density lipoprotein (HDL) cholesterol concentrations were measured using a commercially available kit (Asan pharmaceutical, Seoul, Korea). Low-density lipoprotein (LDL) cholesterol was calculated by the Friedewald formula (total cholesterol - high density lipoprotein (HDL) - cholesterol - triglyceride / 5)}.

(HDL) cholesterol and low density lipoprotein (LDL) cholesterol in the serum of the experimental rats after oral administration of 50% beef tallow high fat diet for 8 weeks. The contents are shown in Table 6. The total cholesterol content of the HFD group was 125.31 ± 20.58 mg / dL, and the oral administration of 250 μL of the mixed hot water extract of Yeoju decreased to 97.48 ± 4.06 mg / dL. The neutral lipid content was significantly lower in the HFD BM250 group than in the HFD BM100 group, which was 104.59 ± 6.57 mg / dL in the HFD group, 114.42 ± 9.62 mg / dL in the HFD BM100 group, and 94.67 ± 14.01 mg / dL in the HFD BM250 group. High density lipoprotein (HDL) cholesterol content was significantly lower in the HFD BM100 group than in the HFD group, with 109.62 ± 12.58 mg / dL in the HFD group, 87.90 ± 10.14 mg / dL in the HFD BM100 group and 101.46 ± 4.95 mg / dL in the HFD BM250 group Respectively. The LDL cholesterol level was significantly lower in the HFD BM100 group (99.05 ± 3.07 mg / dL) and the HFD BM250 group (98.22 ± 4.97 mg / dL) than the HFD group (113.74 ± 4.33 mg / dL) .

Experimental Example 5 Liver lipid content

The liver lipid concentration was determined by repeatedly adding a mixture of chloroform and methanol (2: 1) to the liver tissue supernatant by Folch's method, and the organic solvent fraction was volatilized in the presence of nitrogen gas Total cholesterol and triglyceride were measured with Hua San pharmaceutical kit.

Table 7 shows the contents of total cholesterol and triglyceride in the liver of the experimental rats after oral administration of 50% beef tallow high-fat diet for 8 weeks. The total cholesterol content of the HFD BM250 group was 1.12 ± 0.39 mg / g, which was significantly lower than the HFD group 2.40 ± 0.84 mg / g and the HFD BM100 group 2.55 ± 0.51 mg / g. The neutral lipid content of the HFD BM250 group was 12.96 ± 2.46 mg / g, which was significantly lower than that of the HFD group (33.11 ± 3.71 mg / g) and the HFD BM100 group (34.33 ± 4.23 mg / g)

Experimental Example 6. Serum glutamate oxaloacetate transaminase (GOP) and glutamate pyruvate transaminase (GPT) activity

The activity of serum glutamic acid oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) was measured with a kit (Asan pharmaceutical, Seoul, Korea).

Serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) are enzymes present in hepatocytes. When liver tissue is destroyed, large amounts are released into the blood to increase the level. As a marker of toxicity. Table 8 shows the results of oral administration of 100 μL and 250 μL of the mixed hot-water extract of Yeoju for 8 weeks at a fixed time while consuming the high-fat diet. Glutamate oxaloacetic transaminase (GOT) activity decreased to 46.15 ± 4.16 IU / L in the HFD group and 42.96 ± 1.39 IU / L in the HFD BM250 group, but was not significantly different. Similarly, glutamate pyruvate transaminase (GPT) activity did not show any significant difference in each group. Thus, it was confirmed that the examples do not show toxicity.

[Manufacturing Example]

Production Example 1. Preparation of powder

Yeoju mixed hot water extract powder ........ 3 g

Lactose ............................ 0.5 mg

The above components are powdered and mixed, and filled in an airtight container to prepare a powder.

Production Example 2. Preparation of tablets

Yeoju mixed hot water extract powder ............ 100 mg

Starch ................................ 100 mg

Lactose ................................ 100 mg

Magnesium stearate ................. 1 mg

The above components are mixed and tablets are prepared by tableting according to a conventional method for producing tablets.

Production Example 3. Preparation of capsules

Yeoju mixed hot water extract powder ............ 100 mg

Lactose ................................ 100 mg

Starch ................................ 100 mg

Magnesium stearate .................. 1 mg

The above components are mixed and filled in a gelatin capsule according to a conventional method for manufacturing a cambule to prepare a capsule preparation.

Production Example 4. Preparation of Granules

Yeoju mixed hot water extract powder ............ 10mg

Soybean extract ......................... 50mg

Glucose .............................. 200mg

Starch ................................ 600mg

After mixing the above components, 100 mL of 30% ethanol is added, and the mixture is dried at 60 ° C to form granules, which are filled in a capsule to prepare granules.

Production Example 5. Preparation of a pellet

Yeoju mixed hot water extract powder ........... 1 g

Lactose ............................... 1,500mg

Glycerin ........................... 1000mg

Starch ............................... 980mg

After mixing the above components, they are prepared so as to be 4 g per one ring according to a conventional method for producing a pellet.

Production Example 6. Production of liquid agent

Yeoju mixed hot water extract powder ............ 100 mg

Citric acid .............................. 100 mg

Oligosaccharides ............................ 1 g

Lemon flavor .............................. Suitable amount

Purified water is added to the whole ................ 100 ml

The above components are mixed according to a conventional method for preparing a liquid preparation, filled in a 100 ml brown bottle, and sterilized to prepare a liquid preparation.

Production Example 7. Preparation of Health Functional Foods

Vitamin B acetate (10 mg), Vitamin C (10 mg), Biotin (20 mg), Vitamin A acetate (70 mg), Vitamin E 1.0 mg, Vitamin B1 0.13 mg, Vitamin B 0.15 mg, , Nicotinamide amide 1.7 mg, folic acid 50,, calcium pantothenate 0.5 mg, inorganic mixture q.s., ferrous sulfate 1.75 mg, zinc oxide 0.82 mg, magnesium carbonate 25.3 mg, potassium phosphate monobasic 15 mg, dibasic calcium phosphate 55 mg, 90 mg of potassium citrate, 100 mg of calcium carbonate, and 24.8 mg of magnesium chloride are mixed and prepared according to a conventional method for producing health food.

Production Example 8. Preparation of Health Drink

1,000 mg of citric acid, 10 g of oligosaccharide, 10 g of plum concentrate, 1 g of taurine, and purified water are mixed and mixed according to a conventional method for manufacturing health beverages.

Preparation Example 9. Preparation of confectionery

4 g of Yeoju mixed hot-water extract powder, 62 g of coconut oil, 30 g of sugar, 12 g of egg, 0.5 g of salt and 96 g of wheat flour are mixed and cookies, crackers and snacks are prepared using this mixture.

Production Example 10. Production of Yangeng

Mix 5 g of Yeoju mixed hot-water extract powder, 30 g of sugar, 4 g of agar, 100 g of sediment and 100 g of purified water.

Production Example 11. Production of Dairy Products

5 to 10 parts by weight of a concentrated hot water extract concentrate of Yeoju is added to milk, and the milk is used to produce various dairy products such as butter and ice cream.

Claims (16)

A pharmaceutical composition for anti-obesity comprising hot water extract of Yeoju, Sansui, Omija, and Jujube as an active ingredient.
The method according to claim 1,
Wherein the composition is formulated in a weight ratio of 100: 10 to 15: 5 to 10: 1 to 5: Flaxseed: Corn oil: Omija: Jujube.
The method according to claim 1,
Wherein the jujube is in a weight ratio of 100: 12 to 14: 8 to 10: 3 to 5.
The method according to claim 1,
Wherein the pharmaceutical composition has at least one activity selected from the group consisting of weight loss, reduction of dietary efficiency, decrease of blood lipid content, and decrease of liver lipid content.
An anti-obesity health functional food containing hot water extract of Yeoju, Sansui, Omiza, and Jujube.
6. The method of claim 5,
Wherein the jujube is in a weight ratio of 100: 10 to 15: 5 to 10: 1 to 5.
6. The method of claim 5,
Wherein the jujube is in a weight ratio of 100: 12 to 14: 8 to 10: 3 to 5.
6. The method of claim 5,
Wherein the active ingredient has at least one activity selected from the group consisting of weight loss, reduction of dietary efficiency, decrease of blood lipid content, and decrease of liver lipid content.
An anti-obesity food comprising hot water extract of Yeoju, Sansui, Omija, and Jujube.
10. The method of claim 9,
Wherein the jujube is blended in a weight ratio of 100: 10 to 15: 5 to 10: 1 to 5.
10. The method of claim 9,
Wherein the jujube is in a weight ratio of 100: 12 to 14: 8 to 10: 3 to 5.
10. The method of claim 9,
An anti-obesity food having an activity of at least one selected from the group consisting of weight loss, reduction of dietary efficiency, decrease of blood lipid content, and decrease of liver fat content.
(a) adding and mixing purified water more than twice the weight of the mixture of Yeoju, Sansui, Omija, and Jujube; And
(b) subjecting the mixed solution to hot water extraction at 100 to 125 캜 for 3 hours or more.
14. The method of claim 13,
The method for producing an anti-obesity pharmaceutical composition according to claim 1, wherein in step (a), the ratio is in the range of 100:10 to 15: 5 to 10: 1 to 5.
14. The method of claim 13,
Wherein the jujube is in a weight ratio of 100: 12 to 14: 8 to 10: 3 to 5.
14. The method of claim 13,
The method for preparing an anti-obesity pharmaceutical composition according to claim 1, wherein in step (a), the mixture of Yeast, Sansui, Omija, and Jujube is in the form of a dry powder of each fruit.

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