KR101533910B1 - Composition for preventing and improving hyperlipidemia comprising extracts of Momordica charantia, Corni fructus, Schizandra chinensis and Jujube - Google Patents

Abstract

The present invention relates to a composition for preventing and alleviating hyperlipidemia, containing a mixed extract of Momordica charantia, Corni fructus, Schizandra chinensis Baillon, and Zizyphus jujuba. More particularly, the present invention relates to pharmaceutical and food compositions for preventing and alleviating hyperlipidemia while decreasing total cholesterol and triglyceride and increasing HDL-cholesterol obtained by mixing 70 to 90 wt% of a Momordica charantia extract which is extracted by adding, to Momordica charantia, at least one extraction solvent selected from water, lower alcohol having 1 to 4 carbon atoms, polyhydric alcohol (any one of butylene glycol, propylene glycol, and pentylene glycol), or a mixture thereof; 5 to 15 wt% of a Corni fructus extract; 4 to 10 wt% of a Schizandra chinensis Baillon extract; and 1 to 15 wt% of a Zizyphus jujuba extract.

Description

TECHNICAL FIELD The present invention relates to a composition for prevention and improvement of hyperlipidemia comprising Momordica charantia, Corni fructus, Schizandra chinensis and Jujube,

The present invention relates to a composition for preventing and improving hyperlipidemia, which contains a mixed extract of Yeoju, Sansui, Omija and Jujube. More particularly, the present invention relates to a composition for prevention and improvement of hyperlipidemia, comprising total extracts of Yeoju, HDL-cholesterol is increased to prevent and ameliorate hyperlipidemia.

Hyperlipidemia has become a major chronic disease with high blood pressure and diabetes. According to the statistics of hyperlipidemia patients collected by the Health Insurance Review and Assessment Service, the number of patients increased from 744,000 in 2008 to 1223,000 in 4 years, up by 65%. The number of hyperlipidemia patients, which exceeded one million for the first time in 2010, has risen steadily every year, regardless of age, due to westernized dietary habits.

Hyperlipidemia refers to a condition in which the levels of cholesterol and triglycerides in plasma are higher than normal. Hypercholesterolemia, especially hypercholesterolemia, causes arterial thrombosis and causes thickening of the lipid wall in the blood vessel walls to cause atherosclerosis, which causes blood circulation such as ischemic heart disease, angina pectoris, myocardial infarction and cerebral infarction, It is important to prevent complications.

There are various causes of hyperlipidemia, but hyperlipidemic cholesterol from the food is left when it meets the cell requirement, causing hyperlipidemia while moving along the bloodstream, causing it to be converted into bubble cells with mononuclear cells and megakaryocytes , Which ultimately leads to angina, atherosclerosis or heart attack by blocking the blood flow by thickening the wall in the artery (Chung et al., 2012. Nutr Res. 32, 365-373).

Cholesterol is mainly produced in the liver and is present in the form of lipoprotein in the blood. High density lipoprotein (HDL) among lipoproteins transports cholesterol from liver to liver in other tissues. Therefore, cholesterol is removed from blood vessels and the like when a lot of HDL- In contrast, low density lipoprotein (LDL) is mainly produced in the liver and transports cholesterol to other tissues of the human body. Therefore, when LDL-cholesterol is high, a large amount of cholesterol accumulates in the blood vessels and promotes atherosclerosis. Is present in the form of LDL-cholesterol.

Diet, lifestyle and drug therapy are used for prevention and treatment of hyperlipidemia. The most common treatment methods are dietary and exercise therapy as well as taking various functional food or taking a therapeutic agent for hyperlipemia. Therefore, studies for the development of various functional foods and new drugs for the prevention and improvement of hyperlipemia are being actively carried out.

Cholestyramine and cholestipol, which can lower LDL-cholesterol to 20-35% in the blood, and statin (HMG-CoA Reductase inhibitor) drugs are cholesterol The major step in the synthesis of HMG-CoA to mevalonic acid is inhibited to reduce the amount of cholesterol in the hepatocyte, resulting in increased LDL receptors on the surface of hepatocytes, resulting in 18-55% of blood LDL cholesterol, 7-30% And increases HDL cholesterol by 5-15%. Statins such as lovastatin and simvastatin have been developed and marketed.

However, even if the blood cholesterol level returned to normal level by taking the drug, if you stop taking the drug, your blood cholesterol level may return to normal or may rise. And long-term use of these drugs is often accompanied by serious side effects, so there are few studies on finding substances for treating hyperlipidemia in natural products with fewer side effects.

In addition, since the prevention of hyperlipidemia and early complications are most important, it is possible to prevent the hyperlipemia by inventing beverages that can be ingested daily by using the materials that have been ingested from the past together with the dietary therapy. Therefore, It will be a choice. In addition, the ingestion of beverages, which have been invented using materials used for meals or food for example, may minimize the likelihood of side effects or resistance to drugs.

As a study on the treatment of hyperlipidemia using natural products, Korean Patent Publication No. 2010-0089910 discloses a composition for preventing and treating hyperlipidemia and diabetic hyperlipidemia using extracts of bovine bark, gingival fibrosis, -0037452 discloses a herbal composition for prevention and treatment of hyperlipidemia and angina using herbal medicine such as sea cucumber and perineum, and Korea Patent Publication No. 2004-0027100 discloses a composition for treating hyperlipidemia, which contains a rhubarb extract.

However, there have been no studies or inventions on the prevention and improvement of hyperlipidemia of mixed extracts of Yeoju, Sansui, Omija and Jujube.

Accordingly, the inventors of the present invention have completed the present invention by confirming that the total cholesterol and triglyceride are decreased and the HDL-cholesterol is increased when the mixed extract of Yeoju, Sansui, Omija and Jujube is fed to an adult pre-hyperlipidemia Respectively.

Accordingly, it is an object of the present invention to provide a pharmaceutical composition and a food composition which can prevent and ameliorate hyperlipidemia without adverse effects on human body by using natural materials such as Yeoju, Sansui, Omija and Jujube.

In order to accomplish the above object, the present invention provides a pharmaceutical composition for preventing and / or improving hyperlipidemia comprising water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol (any one of butylene glycol, propylene glycol, and pentylene glycol) 70 to 90% by weight of a laxative extract obtained by adding and extracting at least one extraction solvent selected from the group consisting of a mixture of Lactococcus aureus and a mixture thereof, 5 to 15% by weight of a corn oil extract, 4 to 10% by weight of an Omija extract and 1 to 5% .

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According to another aspect of the present invention, there is provided a food composition for preventing and improving hyperlipidemia comprising water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol (any one selected from the group consisting of butylene glycol, propylene glycol and pentylene glycol ) Or a mixture thereof, 70 to 90% by weight of the extract, 5 to 15% by weight of an extract of corn oil, 4 to 10% by weight of an extract of Omija and 1 to 5% by weight of a jujube extract .

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Wherein the food composition is a health functional food formed of any one form selected from beverage, granule, powder and ring.

The intake of mixed extracts of Yeoju, Sansui, Omija, and Jujube decreases the total cholesterol and triglyceride levels in adults who are pre-hyperlipidemic and increases the HDL-cholesterol level to restore normal lipid levels, thereby preventing progression to hyperlipidemia Or improve the performance of the system.

Accordingly, the present invention can provide a pharmaceutical composition and a food composition effective for preventing and improving hyperlipidemia, and can be usefully utilized as a functional natural material in various fields.

In addition, in the present invention, it was confirmed that when the mixed extract of Yeoju, Sansui, Omija, and Jujube is supplemented with the hyperlipidemia-improving meal, it is possible to more effectively prevent or ameliorate hyperlipemia.

1 is a photograph showing the diet of hyperlipidemia-improving meal,
(AHLMCSJ 0), 7 days after the start of the experiment (AHLMCSJ 7), 10 days after the start of the experiment (AHLMCSJ 10) and 15 days after the start of the experiment (AHLMCSJ 15) A graph showing changes in total cholesterol levels in the blood,
(AHLMCSJ 0), 7 days after the start of the experiment (AHLMCSJ 7), 10 days after the start of the experiment (AHLMCSJ 10), and 15 days after the start of the experiment (AHLMCSJ 15) FIG. 2 is a graph showing the change in the high density lipoprotein-cholesterol (HDL)
(AHLMCSJ 0), 7 days after the start of the experiment (AHLMCSJ 7), 10 days after the start of the experiment (AHLMCSJ 10), and 15 days after the start of the experiment (AHLMCSJ 15) This is a graph showing changes in blood triglyceride levels.

Hereinafter, a composition for preventing and improving hyperlipemia according to a preferred embodiment of the present invention will be described in detail.

The composition for preventing and improving hyperlipidemia according to one embodiment of the present invention is a pharmaceutical composition.

The pharmaceutical composition for prevention and improvement of hyperlipidemia according to the present invention contains as an active ingredient, a mixed extract of Yeoju extract, Sansori oil extract, Omiza extract and Jujube extract.

Momordica charantia ) is a fruit of the vine plant of cucurbitaceae, also called bitter melon in English name. As a physiologically active substance of Yeoju, momordicin, charantin and conjugated linoleic acid belonging to plant sterols are known (Sathishsekar D and Subramanian S. 2005. Biol. Pharm. Bull 28, 978-983). In the case of China, India, and East Asia, Yeoju is used as a major functional vegetable (Park et al., 2007. Korean J. Medicinal Crop., 15, 56-61, Ji et al. Nutr 39, 203-209).

Corni fructus ) is a fruit of the seedling tree which is a deciduous tree of the debris tree, and becomes a red fruit when ripe. It is used as a material for alcohol or tea, and it is also used for medicinal use (Lee et al. 2012. J Korean Soc. Food Science Nutr 41, 390-395). It has been used for the purpose of treating polyuria, back pain, tinnitus, pulmonary tuberculosis treatment, hypertension, anticancer, antimicrobial and neuroleptic treatment in oriental herbs (Seo et al., 1999. Korean J. Postharvest Sci. Technol. 32, 829-832), antimicrobial and antioxidant activities (Kim, 2005. J. Korean Oil Chemists' Soc. 22, 157-167, Kim et al., 2003. J. Korean Soc. Food Sci. Allergic effects (Seo et al. 2002. Kor J. Herbology 17, 1-12). A study on the development of Korean traditional tea using corn oil and gugija (Joo, 1998. Korean J. Dietary Culture 3, 377-383), Development of Yakju using corn oil (Lee et al 2008. Korean J. Food Sci. 1-10), etc. As can be seen, corn oil is a food material that has been edible in the diet.

Schizandra chinensis Baillon) is a fruit of magnolia and plant. It is used as a raw material for food such as herbal medicine materials, tea, and green lacquer materials in the summer season, or starch desserts and omija using these pigments. It has been reported that omega contains a large amount of organic acids and anthocyanin pigments (Kim et al. 1973. Korean J. Food Sci. Technol 5, 178-182), aging inhibitory activity (Nishiyama et al. Bull., 19, 388-393), immunomodulatory and antimicrobial activities (Li et al., 1990. Fee Radic. In addition, a study on the production of vinegar using Omija (Lim and Sul. 2004. J. East Asian Soc. Dietary Life 14, 508-512; Mo et al. 2013. J Korean Soc. Food Sci. 449). As can be seen from these reports, omija has been used as a foodstuff for various purposes.

Jujube ( Zizyphus) jujuba ) is a deciduous broad-leaved arboreous tree of the genus Zizyphus (Rhamnace). It is the origin of North Africa and Western Europe. It has been reported that jujube is an English name of jujube and various sterols, alkaloids, saponins, vitamins, serotonins, organic acids, fatty acids, polyphenols, hydroxymethylpuffural, flavonoids and amino acids are reported as medicinal ingredients (Zryaev et al, 1977. Khim, Prir, Soedin, USSR, 2, 239-243, Okamura et al., 1981. Chem. Pharm. Bull., 29, 675-683). Jujube has long been used as a health food and has been used as a medicinal herb, and jujube has been used as a medicinal product (Na et al. 1996. J. Korean Soc. Food Sci. Nutr. 25, 839-845) Al and Reza et al 2010. Food Chem. Toxicol 48, 639-643), tuberculosis, bronchitis and neuroleptic treatment effects (Lee and Cho 2003. J. Resource Development 22, 23-29) , 1995. J. Korean Soc. Food Nutr. 24, 127-132). Jujube has been used as an over-the-counter medicinal product because it has no adverse effects even when overdosed and contains a large amount of health functional substances (Kim et al., 2013, J. Food Sci., Nutr 42, 1242-1248).

As described above, Yeoju, Sansui oil, Omija, and Jujube used in the present invention are natural ingredients that do not cause side effects and tolerance to patients even for long-term ingestion because they are used as foods for a long time.

The present invention uses mixed extracts of Yeoju extract, Sansui oil extract, Omiza extract and Jujube extract so that each effect of Yeoju, Sansui oil, Omija and Jujube will be further enhanced to prevent and improve hyperlipidemia.

An example of the mixed extract is a mixture of 70% to 90% by weight of the extract, 5% to 15% by weight of the extract of corn oil, 4% to 10% by weight of the extract, and 1% to 5% by weight of the jujube extract. Within the above range, the mixing of the extract of L. japonica, the extract of Cornus sylvestris, the extract of Omiza, and the jujube extract may have an excellent effect of preventing and improving hyperlipidemia.

Extracts can be extracted in a variety of ways. For example, in order to obtain an extract of Yeoju, an extraction solvent may be added to the Yeoju to extract hot water, cold-water or warm-up extract. In this case, the extractant is mixed with the extraction solvent at a weight ratio of 2 to 20 times, and the mixture is extracted at 10 to 150 ° C for 1 to 24 hours. The extraction solvent may be at least one selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, and mixtures thereof. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.

In addition, the extract can be obtained by using reflux cooling extraction, ultrasonic extraction, supercritical fluid extraction, or the like. In addition to the above-described extraction method, an extract obtained through a conventional purification process is also included. For example, an active fraction obtained through various purification methods, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies, etc., is also included in the extract.

The extract of corn oil, Omiza extract, and jujube extract can be extracted in the same manner as described above.

The above-mentioned mixed extracts were obtained by extracting Yeoju extract, Corn oil extract, Omiza extract and Jujube extract and mixing them at a certain ratio. However, in case of mixing Yeoju, Sansui, Omija and Jujube at a certain ratio, Of course. For example, an extraction solvent may be added to a mixture of 70 to 90% by weight of flax, 5 to 15% by weight of marine oil, 4 to 10% by weight of omija and 1 to 5% by weight of jujube, and the mixture may be subjected to hot water extraction, In this case, the mixture may be mixed with the extraction solvent at a weight ratio of 2 to 20 times, and the mixture may be extracted at 10 to 150 ° C for 1 to 24 hours to obtain a mixed extract. The extraction solvent and extraction method are the same as described above.

The pharmaceutical composition for preventing and ameliorating hyperlipemia of the present invention can be administered to mammals including livestock, human and the like by various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

The pharmaceutical composition of the present invention may be prepared by adding appropriate pharmaceutically acceptable carriers, excipients, diluents and the like to the mixed extract to prepare oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions and syrups, injections, inhalants, It can be used in the form of a patch or the like. In this case, the mixed extract may be contained in an amount of 0.01 to 99% by weight based on the total weight of the pharmaceutical composition. Preferably 0.05 to 50% by weight.

When prepared for oral administration, for example, binders (e.g., pregelatinized corn starch, polyvinylpyrrolidone, hydroxypropylmethylcellulose, etc.); Fillers such as lactose, microcrystalline cellulose, calcium phosphate, and the like; Lubricants such as magnesium stearate, talc, silica, etc., disintegrants such as potato starch, sodium starch glycolate, and the like; Or a pharmaceutically acceptable excipient such as a wetting agent (e.g., sodium lauryl sulfate and the like). Typically, it is in the form of solid dosage forms such as granules, powders, tablets, capsules, etc. Tablets or capsules can be coated by methods known in the art.

Liquid preparations for oral administration may take the form of, for example, solutions, syrups, emulsions, or suspensions, and may be presented as a dry product for constitution with water or other suitable vehicle before use.

Such liquid preparations may optionally contain pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, methylcellulose, hydroxy-propylmethylcellulose, hydrogenated edible fats and the like); Emulsifiers (e.g., lecithin, gum arabic, etc.); Non-aqueous vehicles such as almond oil, oily esters, ethyl alcohol, and the like; Preservatives (e.g., methyl or propyl p-hydroxybenzoate, sorbic acid, etc.), and may be prepared by conventional methods. Pharmaceutically acceptable sweeteners preferably include at least one sweetener such as saccharin, sodium saccharin, calcium saccharin, asphaltene, acesulfame potasium, sodium cicamate, alitam, dihydrochalcone sweetener, monelin, Stevioside or sucralose (4,1 ', 6'-trichloro-4,1', 6'-trideoxygalactosucrose), any bulk sweetener such as sorbitol, mannitol, fructose, Such as sucrose, sodium saccharin, calcium saccharin, and any bulk sweetener such as sorbitol, mannitol, fructose, and the like, such as sucrose, glucose, hydrogenated glucose syrup, xylitol, caramel or honey, . ≪ / RTI >

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art. For example, 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. The administration may be carried out once a day or divided into several times.

Meanwhile, another embodiment of the present invention is a food composition having an effect of preventing and improving hyperlipidemia, comprising a mixed extract extracted from a mixture of Yeoju, Sansui, Omija and Jujube as an active ingredient.

The amount of the mixed extract may be 0.1 to 90% by weight of the total food composition, and may be less than the above range for health and hygiene purposes or long-term intake for health control purposes. It is not limited to the above range since it can be used in an amount of more than the above range because there is no problem.

The term "food composition" as used herein means a natural product or a processed product containing one or more nutrients. Preferably, the food composition is in a state in which it can be directly eaten through a certain processing step. , Food, food additives, functional foods and beverages.

Foods according to the present invention include, for example, various foods, beverages, gums, tea, vitamin complexes, and functional foods. In addition, the food may be selected from the group consisting of special nutritional foods (eg crude oil, milk, baby food, etc.), meat products, fish products, tofu, jelly, noodles (Eg soy sauce), candy, chocolate, gum, ice cream, dairy products (eg fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (E.g., various kinds of kimchi, pickles, etc.), beverages (e.g., fruit drinks, vegetable beverages, beverages, fermented beverages, etc.) and natural seasonings (e.g., ramen soup and the like). The food can be prepared by a conventional production method.

Furthermore, the present invention provides a flavor enhancer and a flavor enhancer which can be suitably used as flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and fillers (cheese, chocolate etc.), pectic acid and its salts, alginic acid and its salts, Stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, etc. These components may be used independently or in combination.

Preferably, the food composition can be provided as a health functional food. Here, "health functional food" refers to a food group that gives added value to the function of the food by using physical, biochemical, biotechnological techniques, etc. to control the function of the food, Means a food which is designed and processed so that the body's control function regarding recovery and the like is sufficiently expressed in living bodies.

The health functional food may include a food-acceptable food-aid additive, and may further include suitable carriers, excipients and diluents conventionally used in the production of functional foods.

The health functional food can be formulated into various forms, and the form thereof is not particularly limited. Preferably, the health functional food may be formed into any one form selected from beverage, granule, powder, and ring. Healthy functional foods having such beverages, granules, powders, and ring formulations are easy to carry and are easily ingested at any time and anywhere.

As one example of the formulation of the health functional food, the beverage may be 1-80% by weight of the mixed extract and 20-99% by weight of the purified water. Further, taurine, citric acid, vitamin C, carbohydrates and the like may be further added as an additive to beverages. The carbohydrate may be a monosaccharide such as glucose, fructose, etc .; Disaccharides such as maltose, sucrose and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and xylitol, sorbitol, erythritol, and the like. In addition, various flavorings such as ordinary beverages may be contained as additional ingredients. Natural flavoring agents such as tau martin and stevia extract can be used as flavorings.

The formulations such as granules, powders, and rings contain 10 to 80% by weight of the mixed extract, and may further contain other adhesive, spice, vitamin, carbohydrate and the like.

For example, a mixture of 40% by weight of a ring-mixed extract, 50% by weight of a pressure-sensitive adhesive, 8% by weight of a carbohydrate and 2% by weight of a spice is kneaded and kneaded in a conventional ventilator, And dried. As the pressure-sensitive adhesive, starch syrup, honey, and flour paste may be used.

It is of course possible to form granules having a diameter of 0.5 to 1.5 mm by using a granulator instead of the ventilation. In addition, the granules may be extruded and granulated in an appropriate amount to prepare tablets, or they may be filled into capsules to form capsules.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

<Selection of mixed extract>

The reactive oxygen species (ROS) is increased by the causes of cell senescence, smoking, obesity, insulin resistance, etc., resulting in overall cytotoxicity, leading to aging and various disease pathologies (Valko, M. et al., Free radicals, metals and antioxidants in oxidative stress-induced cancer. 10, pp. 1-40, 2006). Thus, more than 90% of the damage caused by reactive oxygen species causes various diseases such as various cancers, strokes, arteriosclerosis, diabetes, general inflammation, and Parkinson's disease. Therefore, we aimed to find the best extracts for the prevention and improvement of hyperlipemia based on the antioxidant activity of extracts of Yeoju, Sansui, Omija and Jujube extracts and their mixed extracts.

Each of water, milk powder, omija, and jujube was added 10 times by weight of water, followed by hot water extraction at 110 ° C for 4 hours, followed by filtration through a filter paper to obtain Yeast extract, Corn oil extract, Omiza extract and Jujube extract.

In order to investigate various pharmacological effects of Yeoju, Yeoju extract was selected as a main ingredient, and then mixed extracts were prepared by adding at least one extract of corn oil extract, Omiza extract and jujube extract. The mixed extracts prepared by various blends and ratios were analyzed by sensory evaluation, and then the following three mixed extracts with favorable taste were used for the experiment.

The first mixed extract was prepared by mixing 80% by weight of Yeast extract, 10% by weight of Sansui oil extract and 10% by weight of distilled water, and the second mixed extract contained 80% by weight of Yeast extract, 10% by weight of Sansui oil extract, 10% , And the third mixed extract was prepared by mixing 80% by weight of Yeast Extract, 10% by weight of Sansui Oil Extract, 7% by weight of Omiza Extract, and 3% by weight of Jujube Extract.

The antioxidative activities of the extracts of Yeoju, Sanswort, Omija, Jujube, 1st, 2nd and 3rd mixed extracts were investigated.

Each sample was filtered through a microfiltration filter and a filter paper (Whatman, No. 2) to remove solids, and then concentrated to 50 mL (Tokyo rkiakikai, N-1000, Japan), diluted with distilled water to a concentration of 2 mg / mL .

1.2 ml of DPPH (1,1-diphenyl-2-picryl hydrazyl) of 1.5 × 10 ^-4 M was added to 1.8 ml of the sample, reacted at 37 ° C. for 30 minutes, and the absorbance was measured at 517 nm. The radical scavenging activity was expressed by the absorbance reduction rate of the sample and the control. The control group used distilled water instead of the sample. The radical scavenging activity was calculated by the following equation and is shown in Table 1 below.

Radical scavenging activity (%) = (1-absorbance of sample added / absorbance of control) × 100

division Radical scavenging performance (%) Yeoju extract 5.5 Corn oil extract 67.1 Omija extract 42.6 Jujube extract 86.3 First mixed extract 83.9 Second mixed extract 89.6 Third mixed extract 92.5

The results of Table 1 show that the radical scavenging activity of Yeoju extract, Cornus japonica Extract, Omiza extract and Jujube Extract was 5.5, 67.1, 42.6 and 86.3%, respectively. The extracts showed the lowest antioxidative effect and the jujube extract showed the highest antioxidative effect.

The radical scavenging activity of the first mixed extract was 83.9% and the radical scavenging activity of the second mixed extract was 89.6%. The radical scavenging activity of the third mixed extract was 92.5%. From the above experiment, the antioxidative effect of the third mixed extract was most excellent among the three mixed extracts. In addition, the antioxidative effect of mixed extracts was higher than that of Yeoju extract, Sansori oil extract, Omiza extract and Jujube extract. In particular, the third mixed extract had the highest antioxidative effect compared to the extracts of the respective alone and the first and second mixed extracts, even though the extract contained 80 wt% of the lowest extract. It is presumed that mixing four kinds of extract greatly increases antioxidant activity by synergy effect.

Hereinafter, the experiment was conducted using the third mixed extract, which was confirmed to have the highest antioxidant activity.

<Total polyphenol content of mixed extracts>

The total polyphenol compound content of the mixed extract consisting of 80% by weight of the extract of Yejoo, 10% by weight of the extract of corn oil, 7% by weight of Omiza extract and 3% by weight of the jujube extract was determined by applying the Folin-Denis method (Gutfinger, 1958) .

0.5 mL of Foline-Ciocalteau's phenol regent was added to 5 mL of the mixed extract, and the mixture was allowed to stand at room temperature for 3 minutes. Then, 1 mL of a saturated solution of 10% Na ₂ CO ₃ was added and reacted at room temperature for 1 hour, and the supernatant was measured for absorbance at 700 nm. The standard solutions were prepared by preparing caffeic acid at a concentration of 0-100 μg / mL and analyzed in the same manner. The total polyphenol content of the mixed extracts was calculated from the standard calibration curves and is shown in Table 2 below.

division Total polyphenol (mg%) Mixed extract 85.58 ± 3.95

Referring to the results shown in Table 2, the total polyphenol content of the mixed extract consisting of 80% by weight of Yeast extract, 10% by weight of Sansui oil extract, 7% by weight of Omiza extract and 3% by weight of jujube extract was 85.58 ± 3.95 mg% appear. Total polyphenol content correlated positively with antioxidant activity (Kim et al. 2010. J. Korean Soc. Food Sci., 39, 193-202). Therefore, the results of total polyphenol measurements are consistent with the high antioxidant activity of mixed extracts.

<Turbidity, pH and sugar content of mixed extract>

The turbidity of the mixed extract consisting of 80% by weight of Yeoju extract, 10% by weight of corn oil extract, 7% by weight of Omiza extract and 3% by weight of jujube extract was measured at 660 nm and the pH was determined by filtering the mixed extract, Respectively. The sugar content (° Brix) was measured with a sugar meter. The experimental results are shown in Table 3 below.

division Measures Turbidity 0.44 + 0.02 pH 4.38 + 0.02 Sugar content 1.8 ± 0.00

Referring to Table 3, the turbidity of the mixed extract was 0.44 at 660 nm, pH was 4.38, and it was found to be slightly acidic, and the sugar content was 1.8 ° Birx.

As a result of the results shown in Table 3, the possibility of developing a beverage containing a mixed extract as an example of a food composition for preventing and improving hyperlipidemia was confirmed.

<Lipid effect test in blood>

Contemporary high-fat diets increase obesity by accumulating body fat, increase triglycerides, total cholesterol, and LDL-cholesterol, known to be harmful cholesterol, and lower the beneficial cholesterol, HDL-cholesterol. Increased blood LDL-cholesterol has been shown to be a major cause of cardiovascular disease and can lead to high mortality (S. M. Grundy, Cholesterol and coronary heart disease, JAMA, 256, 2849-2858 (1986)). Metabolic diseases caused by abnormality of energy metabolism in the body may cause hyperlipemia caused by differentiation of adipocytes and regulation of lipid metabolism.

In this experiment, the effect of the mixed extract of 80% by weight of Yeoju Extract, 10% by weight of Sansui Extract, 7% by weight of Omiza Extract and 3% by weight of Jujube Extract, Let's look at it.

1. Subject

The diagnosis of hyperlipidemia is made by comparing the total cholesterol, HDL-cholesterol and triglyceride levels with the baseline values by fasting blood test. When total cholesterol is less than 200 mg / dL, it is normal, and when it is 200 to 239 mg / dL, it is pre-hyperlipidemia (no health problem, but self-management and preventive measures). And HDL-cholesterol is normal at over 60 mg / dL, pre-hyperlipidemia at 40 to 59 mg / dL, normal triglyceride at less than 150 mg / dL and pre-hyperlipidemia at 150-199 mg / dL.

The subjects were 9 adult applicants with total cholesterol of 200 ~ 239 mg / dL, HDL-cholesterol of 40 ~ 59 mg / dL and triglycerides of 150 ~ 199 mg / dL as pre-hyperlipidemia.

2. Experimental Method

A schematic diagram of the experimental method is shown in Table 4 below.

Experiment period (days) 0 One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Blood sampling Blood sampling Blood sampling Blood sampling 100 ml of mixed extract after normal meal (breakfast, lunch, dinner) Normal meal (morning and evening), hyperlipidemia improvement meal (lunch) 100 ml of mixed extracts intake

The experiment was carried out for a total of 15 days. During the experiment, three meals were made every morning, lunch and dinner, and 100 mL of mixed extract was taken after each meal.

From the 11th day to the 15th day, normal meals were used in the morning and evening, and the lunch was used for the improvement of hyperlipemia (Table 5 and Fig. 1 ) Were used.

Blood samples were taken before and 7, 10, and 15 days after the experiment. 1 mL of the blood of the subject was immediately taken in the ice box with the help of a hospital specialist, and then transferred to the laboratory, centrifuged (X3,000 rpm, 20 min) and stored at -20 ° C.

The hyperlipidemic diet was taken at the designated time (12:00) after cooking at the Gourmet Office of Gwangju University. The diet of hyperlipidemia-refractory meals is summarized in Table 5 below.

food
Materials and Quantity (g) Grains group
Fishery group Vegetable group
Provincial
Milk group
Fruit group
that medium Brown rice Brown rice 60 2 - - - - - - Bean sprouts radish soup

No 20 0.4 Sprouts 35 0.5 The domestic anchovy 7.5 0.5 Grilled mackerel
Mackerel 50 One Edible oil 5 One Cabbage salad dressing
Cabbage 35 0.5 Mayonnaise 5 One Cucumber Cucumber 35 0.5 Apple Apple 80 0.5 One

The diets of Table 5 were prepared using the food exchange table. Foods used in the hyperlipidemia-improving diet were classified into six food groups having similar nutrient composition, namely, cereals group, fish group, vegetable group, fat group, milk group, The exchange units representing the nutrient content for each food group are listed.

The reference unit quantity of cereal group '1 exchange' is caloric value 100kcal, saccharide 23g and protein 2g. Therefore, the cereal group of the hyperlipemia-improving dietary diet shown in Table 5 is 2 exchange units, meaning that when the rice rice is consumed, it consumes 200 kcal of calories, 46 g of saccharides and 4 g of protein.

For reference, 1 exchange unit of low-fat (low) fish meat group is 50kcal of heat, 8g of protein, 2g of fat, 1 exchange unit of meat in stopper room (medium) is 75kcal of heat, 8g of protein, 5g of fat, Is a calorie of 20kcal, a saccharide of 3g, a protein of 2g. The fat 1 exchange unit is 45kcal of calories and 5g of fat. The exchange unit of fruit group 1 is 50kcal of calories and 12g of saccharide.

3. Measurement of total cholesterol, HDL-cholesterol and triglyceride in blood

Total cholesterol, HDL-cholesterol, and triglyceride were measured in the blood from the subjects before the experiment (AHLMCSJ0), 7 days after the start of the experiment (AHLMCSJ7), 10 days (AHLMCSJ10) and 15 days (AHLMCSJ15). The contents of total cholesterol, HDL-cholesterol, and triglyceride in the blood were quantitatively determined as an enzyme kit (Asan Pharmaceutical, Korea) prepared for commercial use, and the results are shown in Figs. 2 to 4.

Referring to FIGS. 2 to 4, the total cholesterol and triglyceride levels decreased as the intake period of the mixed extracts increased during the experimental period (FIGS. 2 and 4), and HDL-cholesterol HDL-cholesterol) levels increased (FIG. 3). The increase in triglyceride and total cholesterol has been shown to increase the incidence of cardiovascular diseases such as myocardial infarction and atherosclerosis, and the increase in HDL-cholesterol is known to help prevent it (J. Jensen., B. Anette., D. Steen., H. Kjeld, and H. Gunhild, The effect of palm oil, lard and puff-pastry on margarine on postprandial lipid and hormone responses in normal-weight and obese young women, J. Nutr, 82, 469 -479 (1999)).

The results of the above experiment showed that when the mixed extracts of Yeoju, Sansui, Omija and Jujube were administered to the subjects in the pre-hyperlipidemia, the effect of preventing and improving hyperlipemia was evaluated as follows: total cholesterol, HDL-cholesterol and triglyceride .

The total cholesterol and triglyceride levels were lower and the HDL-cholesterol level increased as the period of intake of the mixed extract increased. Adults with pre - hyperlipidemia did not develop hyperlipidemia and recovered to normals by ingestion of mixed extracts. And this effect tended to be more pronounced when mixed extracts were supplemented with a hyperlipidemic diet. Therefore, it is expected that hyperlipidemia can be prevented and improved even if mixed extract is ingested together with regular meal.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (7)

From 70 to 90% by weight of an extract of Yeast extract obtained by extracting and extracting at least one extraction solvent selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols (any one of butylene glycol, propylene glycol and pentylene glycol) 5 to 15% by weight of corn oil extract, 4 to 10% by weight of Omiza extract, and 1 to 5% by weight of jujube extract. delete delete From 70 to 90% by weight of an extract of Yeast extract obtained by extracting and extracting at least any one extraction solvent selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols (any one of butylene glycol, propylene glycol and pentylene glycol) 5 to 15% by weight of corn oil extract, 4 to 10% by weight of Omiza extract, and 1 to 5% by weight of jujube extract. delete delete The food composition for preventing and improving hyperlipidemia according to claim 4, wherein the food composition is a health functional food formed of any one selected from beverage, granule, powder and ring.

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