KR101536974B1 - Composition for preventing and improving diabetes comprising extracts of Momordica charantia, Corni fructus, Schizandra chinensis and Jujube - Google Patents

Abstract

The present invention relates to a composition for preventing and improving diabetes comprising extracts of momordica charantia, corni fructus, schizandra chinensis, and jujube, and more specifically, to a composition which decrease fasting blood sugar and inhibits a rapid increase in blood sugar after a meal to prevent and improve diabetes. The composition contains extracts of 70-90 wt% of momordica charantia, 5-15 wt% of corni fructus, 4-10 wt% of schizandra chinensis, and 1-5 wt% of jujube, the extracts extracted with an extraction dissolvent, which is either a polyhydric alcohol, which is one from water, low-grade alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, and pentylene glycol, or a mixture thereof. The composition is prepared into one formulation, which is selected from a drink, a granule, a powder, and a pill.

Description

(Composition for preventing and improving diabetes mellitus comprising extracts of Momordica charantia, Corni fructus, Schizandra chinensis and Jujube)

The present invention relates to a composition for prevention and improvement of diabetes mellitus containing mixed extracts of Yeoju, Sansui, Omija and Jujube. More particularly, the present invention relates to a composition for preventing and improving diabetes mellitus by using mixed extracts of Yeoju, And a composition for prevention and improvement of diabetes containing a mixed extract of Yeoju, Sansui, Omija and Jujube, which can prevent and improve diabetes by inhibiting the increase of blood sugar.

Diabetes, one of the largest diseases in the world, has increased more than 12 times in the past 20 years in Korea. In 2025, one in four people are likely to become diabetic, 30% of men in their 50s and 20% (Pre-diabetes), which can lead to diabetes, and the prevalence of diabetes is increasing in women in their twenties due to westernized eating patterns.

Diabetes mellitus is a group of disorders in which metabolic disturbance of glucose occurs due to lack of insulin action. Diabetes can be divided into insulin-dependent diabetes, type 1 diabetes mellitus and type 2 diabetes, which is noninsulin dependent diabetes mellitus.

Type 1 diabetes is caused by insufficient absolute amounts of insulin and type 2 diabetes is hyperinsulinemic due to increased resistance to insulin. Type 1 diabetes accounts for about 10% of all cases, and it is called pediatric diabetes because it usually occurs in younger children under 20 years of age. It can not control blood sugar because of the loss of beta (?) Cells that produce insulin in the body, and type 2 diabetes mainly develops after 40 years of age. This is because insulin levels in the blood are normal or hyperinsulinemic, resulting in hyperglycemia due to abnormal glucose metabolism due to high insulin resistance in the peripheral tissues.

Diabetes is a genetic factor and is affected by obesity, dietary habits, lack of exercise and stress. Diabetes is not a big disease in itself, but complications that arise from a long-term illness, For example, patients suffering from diabetic neuropathy, retinopathy, cataract, and nephropathy may not only be able to lead normal lives, but may also cause fatal consequences. .

The most important goal of diabetes therapy is to adjust blood glucose levels as close as possible to normal levels. After fasting blood sugar, blood glucose control after meals is important for the improvement of diabetic symptoms and prevention and treatment of complications. Therapeutic methods include drug therapy, diet , And exercise therapy.

Diet therapy and exercise therapy are needed to control blood sugar in diabetic patients, and drug therapy is used when these methods do not improve them. Although the postprandial blood glucose is well controlled by the drug, side effects and drug tolerance of the drug are problematic without boiling.

Prevention is the most important because diabetes is a chronic disease that can not be cured by current therapies. Therapeutic therapies lacking scientific evidence are also often used. Therefore, it is urgent to develop natural antidiabetic materials and health foods that can minimize side effects with scientific evidence.

Prevention of diabetes mellitus and its complications are the most important, so it is a wise choice to prevent diabetes by developing foods that can be taken on a daily basis with food that has been ingested since the past. In addition, the intake of foods developed using meals or materials used for edible purposes can minimize the likelihood of side effects or resistance to drugs.

As a study on the treatment of diabetes using natural products, Korean Patent No. 10-0894029 discloses a pharmaceutical composition for prevention of diabetes mellitus and a pharmaceutical composition for treatment using ginseng flower as a raw material, and Korean Patent No. 10-1126677 Discloses a composition for prevention or treatment of diabetes or diabetic complications which comprises the extract of Rootgrass as an active ingredient, and Japanese Patent No. 10-0237974 discloses a confectionery composition for diabetic patients containing the extract of Root Bark, as a main component.

However, there have been no reports on the prevention and improvement of diabetic effects of Yeoju, Sansui, Omija, and Jujube on their extracts.

Accordingly, the present inventors have completed the present invention by confirming that pregestion of diabetic pre-diabetics reduces fasting blood glucose and suppresses rapid postprandial increase in blood glucose when mixed extract of Yeoju, Sansui, Omija and Jujube is ingested.

Accordingly, it is an object of the present invention to provide a composition which can prevent and improve diabetes without adverse effects on the human body by using natural materials such as Yeoju, Sansui, Omija and Jujube.

In order to achieve the above object, the composition for preventing and improving diabetes according to one embodiment of the present invention comprises water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol such as butylene glycol, propylene glycol, or pentylene glycol, 70 to 90% by weight of the extract obtained by extracting at least one extraction solvent selected from the group consisting of a mixture of the extracts of 5 to 15% by weight of the extract of corn oil, 4 to 10% by weight of the extract of Omija and 1 to 5% , Granule, powder, and ring.

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It has been experimentally confirmed that dietary supplementation of Yeoju, Ssangyu, Omija and Jujube can prevent and improve diabetes by lowering fasting blood sugar and 2-hour postprandial blood glucose in adults who are pre-diabetic and suppressing rapid blood sugar increase by eating . In the oral glucose tolerance test, the increase of blood glucose level was delayed by the mixed extract intake and the recovery rate to normal blood glucose was fast.

In particular, the present invention has confirmed that the main active substance of the mixed extract, which results in the blood glucose lowering effect, is carantine.

Accordingly, the present invention can provide a pharmaceutical composition and a food composition effective for prevention and improvement of diabetes using a mixed extract, and can be utilized as a functional natural material in various fields.

FIG. 1 is a summary of the diet of the anti-diabetic diet consumed for 6 days by the ADMCSJ group during the anti-diabetic experiment,
FIG. 2 is a photograph showing the diet of the anti-diabetic meal summarized in FIG. 1,
FIG. 3 is a graph showing fasting blood glucose levels measured on the 7th day (MCSJ 7), 15th day (MCSJ 15), and 23 days (MCSJ 23) of the ADMCSJ group before and after the start of the anti-diabetic test,
FIG. 4 is a graph showing the fasting blood glucose level measured by the Glucose kit on the 23rd day after the start of the experiment in the ADMCSJ group and NDW group,
FIG. 5 is a graph showing the blood glucose level measured by the blood glucose meter within 10 minutes after the meal on the 23rd day after the start of the experiment in the ADMCSJ group and the NDW group,
FIG. 6 is a graph showing the blood glucose level measured by the blood glucose meter after 2 hours from the end of the experiment on the 23rd day after the start of the experiment in the ADMCSJ group and the NDW group,
FIG. 7 is a graph showing the result of oral glucose tolerance test in the fasting state on the 15th day after the start of the experiment in the ADMCSJ group and the NDW group,
FIG. 8 is a graph showing the result of oral glucose tolerance test in the fasting state on the 23rd day after the start of the experiment in the ADMCSJ group and the NDW group,
FIG. 9 is a graph showing the results of analysis of major antidiabetic substances of the mixed extract. FIG.

Hereinafter, the composition for preventing and improving diabetes mellitus according to a preferred embodiment of the present invention will be described in detail.

The composition for preventing and improving diabetes mellitus according to one embodiment of the present invention is a pharmaceutical composition.

The pharmaceutical composition for prevention and improvement of diabetes according to the present invention contains as an active ingredient, a mixed extract obtained by mixing Yeast extract, Sansori oil extract, Omiza extract and Jujube extract.

Momordica charantia ) is a fruit of the vine plant of cucurbitaceae, also called bitter melon in English name. Momordicin, conjugated linoleic acid, etc. belonging to plant sterols are known as biologically active substances of Yeoju (Sathishsekar D and Subramanian S. 2005. Biol. Pharm. Bull. 28, 978-983 ). In the case of China, India, and East Asia, Yeoju is used as a major functional vegetable (Park et al., 2007. Korean J. Medicinal Crop., 15, 56-61, Ji et al. Nutr 39, 203-209).

Corni fructus ) is a long elliptical fruit that changes from green to red in August-October, with a slight sweetness and strong acidity. Flesh and seed are separated, and meat is used as a material for alcohol, tea, and Chinese medicine. Flesh contains fructose such as cornin, morroniside, loganin, tannin, and saponin and organic acids such as wine, malic acid and tartaric acid. It also contains vitamin A and a large amount of sugars.

Schizandra chinensis ) is a fruit of magnolia and plants, and is processed mainly as herbal medicine materials, car or tea materials in summer or summer, and starch desserts and omija using those pigments. Omija contains a large amount of organic acids and anthocyanin pigments (Kim KI et al. 1973. Korean J. Food Sci. Technol. 5: 178-182), aging inhibitory activity (Nishiyama et al. Pharm. Bull. 19, 388-393), immunomodulatory and antimicrobial activities (Li et al., 1990. Fee Radic.

Jujifus ( Zizyphus jujuba ) is a fruit of the deciduous broad-leaved arboreous tree of the Zizyphus of Rhamnace, and it is the origin of North Africa and Western Europe, and there are about 40 species. As the medicinal component of jujube, various sterols, alkaloids, saponins, vitamins, serotonins, organic acids, fatty acids, polyphenols, hydroxymethylpuppurals, flavonoids and amino acids have been reported Okamura, N., et al. (1981) Chem. Pharm. Bull., 29, 675-683) . Jujube has been used as a health food for a long time because it is rich in nutritional ingredients and its medicinal effects such as emollients, diuretics, tonic, bile, gangjeong, recovery of strength, expectorant, and antiinflammatory drug have been reported.

As described above, Yeoju, Sansui oil, Omija, and Jujube used in the present invention are natural ingredients that do not cause side effects and tolerance to patients even for long-term ingestion because they are used as foods for a long time.

The present invention uses a mixture of Yeoju extract, Cornus japonica extract, Omiza extract and Jujube extract so that each effect of Yeoju, Sansui, Omija, and Jujube is added to further improve the prevention and improvement of diabetes.

An example of the mixed extract is a mixture of 70% to 90% by weight of the extract, 5% to 15% by weight of the extract of corn oil, 4% to 10% by weight of the extract, and 1% to 5% by weight of the jujube extract. Within the above range, the mixture of Yeast Extract, Sanswort Extract, Omiza Extract, and Jujube Extract has excellent diabetes prevention and improvement effect.

Extracts can be extracted in a variety of ways. For example, in order to obtain an extract of Yeoju, an extraction solvent may be added to the Yeoju to extract hot water, cold-water or warm-up extract. In this case, the extractant is mixed with the extraction solvent at a weight ratio of 2 to 20 times, and the mixture is extracted at 10 to 150 ° C for 1 to 24 hours. The extraction solvent may be at least one selected from water, lower alcohols having 1 to 4 carbon atoms, polyhydric alcohols, and mixtures thereof. As the lower alcohol having 1 to 4 carbon atoms, methanol, ethanol and the like can be used. As the polyhydric alcohol, butylene glycol, propylene glycol, pentylene glycol and the like can be used. Mixtures of water and lower alcohols, mixtures of water and polyhydric alcohols, mixtures of lower alcohols and polyhydric alcohols, or mixtures of water and lower alcohols and polyhydric alcohols can be used as the mixture.

In addition, the extract can be obtained by using reflux cooling extraction, ultrasonic extraction, supercritical fluid extraction, or the like. In addition to the above-described extraction method, an extract obtained through a conventional purification process is also included. For example, an active fraction obtained through various purification methods, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies, etc., is also included in the extract.

The extract of corn oil, Omiza extract, and jujube extract can be extracted in the same manner as described above.

The above-mentioned mixed extracts were obtained by extracting Yeoju extract, Corn oil extract, Omiza extract and Jujube extract and mixing them at a certain ratio. However, in case of mixing Yeoju, Sansui, Omija and Jujube at a certain ratio, Of course. For example, an extraction solvent may be added to a mixture of 70 to 90% by weight of flax, 5 to 15% by weight of marine oil, 4 to 10% by weight of omija and 1 to 5% by weight of jujube, and the mixture may be subjected to hot water extraction, In this case, the mixture may be mixed with the extraction solvent at a weight ratio of 2 to 20 times, and the mixture may be extracted at 10 to 150 ° C for 1 to 24 hours to obtain a mixed extract. The extraction solvent and extraction method are the same as described above.

The pharmaceutical composition for prevention and improvement of diabetes according to the present invention can be administered to mammals including livestock, human and the like by various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

The pharmaceutical composition of the present invention may be prepared by adding appropriate pharmaceutically acceptable carriers, excipients, diluents and the like to the mixed extract to prepare oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions and syrups, injections, inhalants, It can be used in the form of a patch or the like. In this case, the mixed extract may be contained in an amount of 0.01 to 99% by weight based on the total weight of the pharmaceutical composition. Preferably 0.05 to 50% by weight.

When prepared for oral administration, for example, binders (e.g., pregelatinized corn starch, polyvinylpyrrolidone, hydroxypropylmethylcellulose, etc.); Fillers such as lactose, microcrystalline cellulose, calcium phosphate, and the like; Lubricants such as magnesium stearate, talc, silica, etc., disintegrants such as potato starch, sodium starch glycolate, and the like; Or a pharmaceutically acceptable excipient such as a wetting agent (e.g., sodium lauryl sulfate and the like). Typically, it is in the form of solid dosage forms such as granules, powders, tablets, capsules, etc. Tablets or capsules can be coated by methods known in the art.

Liquid preparations for oral administration may take the form of, for example, solutions, syrups, emulsions, or suspensions, and may be presented as a dry product for constitution with water or other suitable vehicle before use.

Such liquid preparations may optionally contain pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol syrup, methylcellulose, hydroxy-propylmethylcellulose, hydrogenated edible fats and the like); Emulsifiers (e.g., lecithin, gum arabic, etc.); Non-aqueous vehicles such as almond oil, oily esters, ethyl alcohol, and the like; Preservatives (e.g., methyl or propyl p-hydroxybenzoate, sorbic acid, etc.), and may be prepared by conventional methods. Pharmaceutically acceptable sweeteners preferably include at least one sweetener such as saccharin, sodium saccharin, calcium saccharin, asphaltene, acesulfame potasium, sodium cicamate, alitam, dihydrochalcone sweetener, monelin, Stevioside or sucralose (4,1 ', 6'-trichloro-4,1', 6'-trideoxygalactosucrose), any bulk sweetener such as sorbitol, mannitol, fructose, Such as sucrose, sodium saccharin, calcium saccharin, and any bulk sweetener such as sorbitol, mannitol, fructose, and the like, such as sucrose, glucose, hydrogenated glucose syrup, xylitol, caramel or honey, . ≪ / RTI >

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art. For example, 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg per day. The administration may be carried out once a day or divided into several times.

Meanwhile, another embodiment of the present invention is a food composition having diabetes prevention and improvement effect by containing a mixed extract extracted from a mixture of Yeoju, Sansui, Omija and Jujube as an active ingredient.

The amount of the mixed extract may be 0.1 to 90% by weight of the total food composition, and may be less than the above range for health and hygiene purposes or long-term intake for health control purposes. It is not limited to the above range since it can be used in an amount of more than the above range because there is no problem.

The term "food composition" as used herein means a natural product or a processed product containing one or more nutrients. Preferably, the food composition is in a state in which it can be directly eaten through a certain processing step. , Food, food additives, functional foods and beverages.

Foods according to the present invention include, for example, various foods, beverages, gums, tea, vitamin complexes, and functional foods. In addition, the food may be selected from the group consisting of special nutritional foods (eg crude oil, milk, baby food, etc.), meat products, fish products, tofu, jelly, noodles (Eg soy sauce), candy, chocolate, gum, ice cream, dairy products (eg fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (E.g., various kinds of kimchi, pickles, etc.), beverages (e.g., fruit drinks, vegetable beverages, beverages, fermented beverages, etc.) and natural seasonings (e.g., ramen soup and the like). The food can be prepared by a conventional production method.

Furthermore, the present invention provides a flavor enhancer and a flavor enhancer which can be suitably used as flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and fillers (cheese, chocolate etc.), pectic acid and its salts, alginic acid and its salts, Stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages, etc. These components may be used independently or in combination.

Preferably, the food composition can be provided as a health functional food. Here, "health functional food" refers to a food group that gives added value to the function of the food by using physical, biochemical, biotechnological techniques, etc. to control the function of the food, Means a food which is designed and processed so that the body's control function regarding recovery and the like is sufficiently expressed in living bodies.

The health functional food may include a food-acceptable food-aid additive, and may further include suitable carriers, excipients and diluents conventionally used in the production of functional foods.

The health functional food can be formulated into various forms, and the form thereof is not particularly limited. Preferably, the health functional food may be formed into any one form selected from beverage, granule, powder, and ring. Healthy functional foods having such beverages, granules, powders, and ring formulations are easy to carry and are easily ingested at any time and anywhere.

As one example of the formulation of the health functional food, the beverage may be 1-80% by weight of the mixed extract and 20-99% by weight of the purified water. Further, taurine, citric acid, vitamin C, carbohydrates and the like may be further added as an additive to beverages. The carbohydrate may be a monosaccharide such as glucose, fructose, etc .; Disaccharides such as maltose, sucrose and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and xylitol, sorbitol, erythritol, and the like. In addition, various flavorings such as ordinary beverages may be contained as additional ingredients. Natural flavoring agents such as tau martin and stevia extract can be used as flavorings.

The formulations such as granules, powders, and rings contain 10 to 80% by weight of the mixed extract, and may further contain other adhesive, spice, vitamin, carbohydrate and the like.

For example, a mixture of 40% by weight of a ring-mixed extract, 50% by weight of a pressure-sensitive adhesive, 8% by weight of a carbohydrate and 2% by weight of a spice is kneaded and kneaded in a conventional ventilator, And dried. As the pressure-sensitive adhesive, starch syrup, honey, and flour paste may be used.

It is of course possible to form granules having a diameter of 0.5 to 1.5 mm by using a granulator instead of the ventilation. In addition, the granules may be extruded and granulated in an appropriate amount to prepare tablets, or they may be filled into capsules to form capsules.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

<Selection of mixed extract>

The reactive oxygen species (ROS) is increased by the causes of cell senescence, smoking, obesity, insulin resistance, etc., resulting in overall cytotoxicity, leading to aging and various disease pathologies (Valko, M. et al., Free radicals, metals and antioxidants in oxidative stress-induced cancer. 10, pp. 1-40, 2006). Thus, more than 90% of the damage caused by reactive oxygen species causes various diseases such as various cancers, strokes, arteriosclerosis, diabetes, general inflammation, and Parkinson's disease. Therefore, the antioxidant activity of extracts of Yeoju, Sansui, Omija, and Jujube extracts and their mixed extracts were measured to find the best antidiabetic extracts.

Each of water, milk powder, omija, and jujube was added 10 times by weight of water, followed by hot water extraction at 110 ° C for 4 hours, followed by filtration through a filter paper to obtain Yeast extract, Corn oil extract, Omiza extract and Jujube extract.

In order to investigate various pharmacological effects of Yeoju, Yeoju extract was selected as a main ingredient, and then mixed extracts were prepared by adding at least one extract of corn oil extract, Omiza extract and jujube extract. The mixed extracts prepared by various blends and ratios were analyzed by sensory evaluation, and then the following three mixed extracts with favorable taste were used for the experiment.

The first mixed extract was prepared by mixing 80% by weight of Yeast extract, 10% by weight of Sansui oil extract and 10% by weight of distilled water, and the second mixed extract contained 80% by weight of Yeast extract, 10% by weight of Sansui oil extract, 10% , And the third mixed extract was prepared by mixing 80% by weight of Yeast Extract, 10% by weight of Sansui Oil Extract, 7% by weight of Omiza Extract, and 3% by weight of Jujube Extract.

The antioxidative activities of the extracts of Yeoju, Sanswort, Omija, Jujube, 1st, 2nd and 3rd mixed extracts were investigated.

Each sample was filtered through a microfiltration filter and a filter paper (Whatman, No. 2) to remove solids, and then concentrated to 50 mL (Tokyo rkiakikai, N-1000, Japan), diluted with distilled water to a concentration of 2 mg / mL .

1.2 ml of DPPH (1,1-diphenyl-2-picryl hydrazyl) of 1.5 × 10 ^-4 M was added to 1.8 ml of the sample, reacted at 37 ° C. for 30 minutes, and the absorbance was measured at 517 nm. The radical scavenging activity was expressed by the absorbance reduction rate of the sample and the control. The control group used distilled water instead of the sample. The radical scavenging activity was calculated by the following equation and is shown in Table 1 below.

Radical scavenging activity (%) = (1-absorbance of sample added / absorbance of control) × 100

division Radical scavenging performance (%) Yeoju extract 5.5 Corn oil extract 67.1 Omija extract 42.6 Jujube extract 86.3 First mixed extract 83.9 Second mixed extract 89.6 Third mixed extract 92.5

The results of Table 1 show that the radical scavenging activity of Yeoju extract, Cornus japonica Extract, Omiza extract and Jujube Extract was 5.5, 67.1, 42.6 and 86.3%, respectively. The extracts showed the lowest antioxidative effect and the highest extract of jujube extract.

The radical scavenging activity of the first mixed extract was 83.9% and the radical scavenging activity of the second mixed extract was 89.6%. The radical scavenging activity of the third mixed extract was 92.5%. From the above experiment, the antioxidative effect of the third mixed extract was most excellent among the three mixed extracts. In addition, the antioxidative effect of mixed extracts was higher than that of Yeoju extract, Sansori oil extract, Omiza extract and Jujube extract. In particular, the third mixed extract had the highest antioxidative effect compared to the extracts of the respective alone and the first and second mixed extracts, even though the extract contained 80 wt% of the lowest extract. It is presumed that mixing four kinds of extract greatly increases antioxidant activity by synergy effect.

Hereinafter, the experiment was conducted using the third mixed extract, which was confirmed to have the highest antioxidant activity.

<Antidiabetic test>

In this experiment, we observed the change of blood glucose level of mixed extract (MCSJ) composed of 80% by weight of Yeoju Extract, 10% by weight of Sansui Extract, 7% by weight of Omiza Extract and 3% by weight of Jujube Extract, .

1. Subject

Subjects were 12 adult volunteers with pre-diabetes (100-125 mg / dL). The subjects were divided into 2 groups of 6 persons. That is, the first group, the normal diet + water intake group (NDW group), and the second group, anti-diabetic meal + mixed extract intake group (ADMCSJ group).

2. Experimental Method

A schematic diagram of the experimental method is shown in Table 2 below.

Experiment period (days) 0 One 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 ADMCSJ group A diet B diet A diet NDW County C diet

The experiment was performed for a total of 23 days. The NDW group was fed C for 0 (starting the experiment) to 23 days. The ADMCSJ group received A diet from 0 to 15 days, B diet from 16 to 21 days, and A diet from 22 to 23 days. A dietary intake of 100 mL of the mixed extract was obtained after the normal meal (breakfast, lunch, dinner), and the B diet was 100 mL of the mixed extract after the normal meal (morning and evening) and the anti diabetic meal (lunch) , Lunch, dinner), and then taken 100 mL of water. The diabetic diet for 6 days varied from day to day and was taken after cooking at the Gourmet Office, Gwangju University, at the appointed time (12:00). The diet of 6-day anti-diabetic meal is summarized in Fig. 1 and Fig.

3. Fasting blood glucose measurement with blood glucose meter

(MCSJ 0), and on the 7th day (MCSJ 7), 15th day (MCSJ 15) and 23rd day (MCSJ 23) of the experiment, fasting glucose was measured in the ADMCSJ group Lt; SEP &gt; The measurement results are shown in Fig. In FIG. 3, 'MCSJ 0' is the blood glucose measurement value of the ADMCSJ group at the beginning of the experiment, 'MCSJ 7' is the blood glucose measurement value at the 7th day after the start of the experiment of the ADMCSJ group, And 'MCSJ 23' is the blood glucose measurement value at 23 days after the start of the experiment in the ADMCSJ group.

3, the fasting blood glucose level after the start of the experiment was compared with the start date of the experiment (MCSJ 0). As a result, the blood glucose level was remarkably decreased on the 7th day (MCSJ 7), 15th day (MCSJ 15), and 23 days And blood glucose decreased as the duration of experiment increased.

4. Fasting blood glucose measurement with Glucose kit

On the 23rd day after the start of the experiment, fasting blood glucose was measured against the subjects of the ADMCSJ and NDW groups.

Blood samples were taken from each patient under the guidance of a specialist at Gyeonggi University Hospital, and 1 mL of blood was collected. The blood was immediately placed in an icebox, transferred to a laboratory, centrifuged (X3,000 rpm, 20 min) The fasting blood glucose was measured by Glucose kit (Asan Pharma). The measurement results are shown in Fig.

Referring to FIG. 4, fasting glucose levels in the ADMCSJ group consuming mixed extracts with anti-diabetic meal were significantly lower than those in the NDW group that consumed water with regular meals.

5. Measure blood glucose and postprandial 2-hour blood glucose change within 10 minutes after meal

On the 23rd day, the ADMCSJ group and NDW group received the same evening meal (rice, miso soup, kimchi, spinach, bean sprouts, salad, and anchovy) immediately and then mixed extract and water, respectively. The blood glucose was measured within 10 minutes after the meal and 2 hours after the meal with the blood glucose meter [SDICODE free blood glucose measurement system, SD). The measurement results are shown in Fig. 5 and Fig.

Referring to FIG. 5, the ADMCSJ group showed a significant decrease in blood glucose as compared with the NDW group. This result shows that the intakes of the mixed extract directly after the meal can suppress the rapid increase in blood glucose after the meal. In diabetic patients, sudden postprandial hyperglycemia is a serious problem. Mixed extracts can significantly inhibit blood glucose elevation.

Referring to FIG. 6, the ADMCSJ group showed a significant decrease in the blood glucose level 2 hours after the meal compared with the NDW group.

Patients with diabetes usually eat to avoid postprandial hyperglycemia or eat rice brown rice. However, even if diabetic patients eat regular meals, eating a post-meal mixed extract may inhibit rapid blood sugar increase by eating Respectively.

6. Oral glucose tolerance test

The subjects underwent oral glucose tolerance test (OGTT) on the 15th day and 23rd day after the start of the experiment on the subjects in the ADMCSJ and NDW groups. That is, the blood glucose level was measured with a blood glucose meter (SDCD-free blood glucose measurement system, manufactured by SODI Co., Ltd.) before the intake of 75 g of glucose (0 minute) and after 30 minutes, 60 minutes, 90 minutes and 120 minutes after glucose intake . The measurement results are shown in Fig. 7 and Fig.

The ADMCSJ group showed lower blood glucose levels at all time points than the NDW group on both 15th and 23rd day. In particular, the ADMCSJ group had significantly lower blood glucose levels than the NDW group on both the 15th day and the 23rd day after 120 minutes of glucose intake.

<Major antidiabetic analysis of mixed extracts>

Charantin (a mixture of β-sitosterol-β-d-glucoside and 5,25-stigmastadien-3β-ol-β-d-glucoside) lowers insulin resistance-related blood glucose levels and plays a crucial role in insulin secretion It activates the cells and promotes the secretion of insulin, thereby lowering blood sugar. Carantin has been reported to be a mixture of sitosteryl glucoside and stigmasteryl glucoside.

Therefore, we investigated the antidiabetic effect of carantin in mixed extracts.

The carotene analysis was analyzed by HPLC (Alliance Waters, USA; Model Water 269) system equipped with a Phenomenex Luna 5u C18 (4.6 x 250 mm) column. Detectors were UV detector (Alliance Waters, USA) and pump (Model Water 1525). At a rate of 1.0 mL / min. Mixed extract samples were injected in 10 μl and mobile phase was detected in 90% MeOH and UV 205 nm. Charantin was used as a standard substance. The RT of the sample was compared with the retention time of the reference material and the mature time of the standard was compared.

The experimental results are shown in Fig. Figure 9 A is the result of the mixed extract and B is the result of the standard.

Referring to FIG. 9, two peaks were observed in the mixed extract sample. This is consistent with the presence of a mixture of sitosteryl glucoside and stigmasteryl glucoside. The RT of the mixed extracts was 19.855 min and 22.834 min, and RT was the same when compared with the standard material, Charantin. It was confirmed that carantin was contained in the mixed extract, The effect is thought to play an important role in carantin. Carantine was quantitatively determined to contain 1.25 μg / mg of carantine in the mixed extract.

Fasting blood glucose test, postprandial glucose test and oral glucose tolerance test are mainly used for diabetes diagnosis. In particular, the oral glucose tolerance test is used to confirm diabetes and gestational diabetes due to its high temporal resolution of metabolism and high diagnostic accuracy. In the oral glucose tolerance test, 75 g of glucose solution was administered within 5 minutes after 8 to 14 hours of fasting, and venous blood was collected for 2 hours at intervals of 30 minutes. The results were collected by the World Health Organization (WHO) and the American Diabetes Data Group (NDDG) It is a way to diagnose diabetes by reference.

The results of the above-mentioned experiment showed that 80% by weight of the extract, 10% by weight of the extract of corn oil, 7% by weight of the extract of Omija, 7% by weight of jujube extract 3 The effect of dietary supplementation on weight gain was confirmed by fasting glucose, postprandial glucose and oral glucose tolerance test.

Mixed extracts can reduce fasting blood glucose, 2 hour postprandial blood glucose and prevent rapid blood sugar increase by meals in pre-diabetic adults. In the oral glucose tolerance test, the increase in blood glucose level was slowed by the intake of M mixed extract and the recovery rate to normal blood glucose was fast. In addition, it was found that carantine was the main substance showing the effect of lowering the blood sugar of the mixed extract.

Accordingly, the present invention can provide a pharmaceutical composition and a food composition which are effective for prevention and improvement of diabetes using mixed extract of Yeast extract, mixed mint oil extract, Omiza extract and jujube extract, and is useful as a functional natural material in various fields Can be utilized.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation. Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (8)

delete delete delete delete 70 to 90% by weight of an extract of Yeast extract obtained by extracting at least one extraction solvent selected from water, a lower alcohol having 1 to 4 carbon atoms, a polyhydric alcohol selected from among butylene glycol, propylene glycol and pentylene glycol, or a mixture thereof, Characterized in that the food is a health functional food formed of any one form selected from beverage, granule, powder and ring mixed at a ratio of 5 to 15% by weight of an extract, 4 to 10% by weight of an Omija extract and 1 to 5% A composition for preventing and improving diabetes, comprising a mixed extract of Yeoju, Sansui, Omija and Jujube. delete delete delete

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