KR20100108869A - Composition containing extract of herbal mixture for prevention or treatment of diabetes - Google Patents

Abstract

PURPOSE: A composition containing a medicinal herb extract mixture for preventing and treating diabetes is provided to reduce blood glucose and to enhance insulin secretion. CONSTITUTION: A composition for preventing and treating diabetes contains a medicinal herb extract mixture of Scutellaria baicalensis, Phellodendron amurensis, Gardeniae Fructus, Coptis chinensis, Schizandrae Fructus, and Polygonatum Officinale. The extract is isolated using water, alcohol or a mixture thereof as a solvent at 50-90°C for 1-24 hours. The diabetes is type 2 diabetes. A health food for preventing and treating diabetes contains a medicinal herb extract mixture of Scutellaria baicalensis, Phellodendron amurensis, Gardeniae Fructus, Coptis chinensis, Schizandrae Fructus, and Polygonatum Officinale.

Description

Composition containing extract of herbal mixture for prevention or treatment of diabetes}

The present invention relates to a composition for preventing and treating diabetes comprising a mixed extract of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade.

Diabetes is a non-communicable chronic disease. Insulin secreted by the pancreatic beta cells (β-cell) is insufficient or does not function properly, and glucose is not used as an energy source in the body and remains high in blood. Is a disease that is excreted in the urine.

Diabetes is one of the important adult diseases all over the world. In Korea, the prevalence rate of diabetes mellitus reaches 7-8% with rapid economic growth, and it is an important cause of death for people in their 60s and 70s.

Diabetes is classified into insulin dependent diabetes mellitus (IDDM) and non-insulin dependent diabetes mellitus (NIDDM), which are largely insulin dependent, depending on the cause of the disease and the method of treating the symptoms.

Type 1 diabetes is a state in which the function of the pancreatic beta cells is reduced due to genetic causes, viral infections, etc., almost no secretion of insulin, and suddenly occurs in the 10s to 20s.

Type 2 diabetes is caused by family history, obesity, stress, eating errors, or drugs that antagonize insulin, etc., and appears well after the age of 40, also called adult diabetes. In fact, the majority of all diabetics are Type 2 diabetics.

Type 2 diabetes is associated with a decrease in insulin activity in peripheral tissues such as liver, muscle, and fat cells, resulting in decreased glucose utilization, and in particular, increased production of glucose in the liver, resulting in elevated blood glucose levels in the beta cells of the pancreas. It is a disease that occurs when there is not enough insulin secretion to control it. In other words, type 2 diabetes occurs because insulin secretion does not follow insulin resistance with reduced insulin action.

In type 2 diabetes, insulin is secreted, but in the peripheral tissues such as liver, muscle, and fat cells where insulin acts, insulin activity decreases and glucose utilization decreases, thereby increasing blood sugar and responding to the pancreatic beta cells continuously. It is accompanied by hyperinsulinemia with insulin secretion [Kadowaki T, Hara K, Yamauchi T, Terauchi Y, Tobe K, Nagai R. 2003. Molecular mechanism of insulin resistance and obesity. Exp Biol Med (Maywood) 228: 1111-1117.

However, even in type 2 diabetes, insulin secretion may not be sufficiently made to increase blood glucose levels in the beta cells of the pancreas when blood sugar levels rise. Type 2 diabetes in Korea corresponds to this case, and in this case, both insulin action and insulin secretion are low, and the symptoms of diabetes are more severe and obesity than in the West [Kim J, Choi S, Kong B, Oh Y, Shinn S. 2001. Insulin secretion and sensitivity during oral glucose tolerance test in Korean lean elderly women. J Korean Med Sci 16: 592-597].

Therefore, for the prevention and treatment of type 2 diabetes in Korea, it is known that it is important to improve insulin action and also increase insulin secretion ability by glucose stimulation.

The first drugs used to treat type 2 diabetes are drugs that promote insulin secretion. This is a drug of the sulfonylurea family, which acts directly on ATP-regulated potassium (KATP) channels in the pancreatic beta cell membranes, closing the channels, thereby depolarizing the cell membranes. And insulin secretagogues that promote insulin secretion by introducing Ca ²⁺ into cells [Krentz AJ, Bailey CJ. 2005. Oral antidiabetic agents: current role in type 2 diabetes mellitus. Drugs 65: 385-411; Quesada I, Soria B. 2004. Intracellular location of KATP channels and sulphonylurea receptors in the pancreatic beta-cell: new targets for oral antidiabetic agents. Curr Med Chem. 11: 2707-2716. However, this drug has a problem in that it can induce hypoglycemia by secreting insulin even if the blood sugar is low by closing the KATP channel regardless of the blood sugar level.

Recently, a new treatment for type 2 diabetes is insulin-sensitive substances that improve insulin action and help normalize blood sugar with small amounts of insulin. Insulin-sensitive substances are known to relieve insulin resistance, a fundamental problem of type 2 diabetes, to normalize blood sugar with a small amount of insulin, and ultimately reduce the amount of insulin required for blood sugar to eliminate hyperinsulinemia [Ciaraldi TP]. , Kong AP, Chu NV, Kim DD, Baxi S, Loviscach M, Plodkowski R, Reitz R, Caulfield M, Mudaliar S, Henry RR. 2002.Regulation of glucose transport and insulin signaling by troglitazone or metformin in adipose tissue of type 2 diabetic subjects. Diabetes 51: 30-36]. In other words, insulin-sensitive substances could alleviate the symptoms of insulin resistance syndrome as well as diabetes, helping to facilitate the signaling system that occurs in the cells after insulin binds to the insulin receptors. To improve.

The secretion of insulin from pancreatic beta cells is indirectly related to insulin / IGF-1 (Insulin like Growth Factor-1) signaling, and the degradation of beta cells is associated with decreased beta cell production and apoptosis. This increases the amount of beta cells, and consequently leads to diabetes by not meeting the need for insulin secretion.

In addition, GLP-1 is a peptide secreted by L-cells of small intestine cells in vivo, and promotes the secretion of insulin by glucose stimulation in beta cells, and increases the amount of beta cells by promoting the growth and suppression of beta cells. It is known to improve the function of beta cells.

Insulin receptor substrate-2 (IRS2), pancreas duodenum homeobox-1 (PDX-1) in isolated Langerhans as a method for improving pancreatic beta cell survival and function And a method of increasing the amount of mRNA of glucokinase. Induction of IRS2 expression is known to be caused by the activation of Min6 cells and the cAMP response element-binding protein (CREB) of Langerhans Island (Hennige, AM et al., J. Clin. Invest. 112: 1521-1532, 2003 Jhala, US et al., Genes Dev. 17: 1575-1580, 2003; Park, S. et al. J. Biol. Chem. 281: 1159-1168, 2006).

A recently developed glucose-stimulated insulin secretagogue is the glucagon like peptide-1 (GLP-1) receptor agonist, exendin-4 (exendin-4, exenatide). There is).

Exendin-4, unlike drugs of the sulfonylurea family, does not act directly on the KATP channel, but binds to the GLP-1 receptor present in the cell membrane and raises the cAMP concentration in the cell, thereby enhancing protein kinase A (PKA). ) Is activated. Not only the active PKA not increase the Ca ²⁺ influx into the cell, when glucose is increased glucose is absorbed into the beta-cell As via glycolysis (glycolysis) due to high concentrations of ATP / ADP is PKA is activated, Ca ²⁺ Increased influx to promote insulin secretion [Drucker DJ. 2001. Development of glucagon-like peptide-1-based pharmaceuticals as therapeutic agents for the treatment of diabetes. Curr Pharm Res 7: 1399-1412]. Therefore, exendin-4 does not promote insulin secretion when blood sugar is normal, but acts as a glucose-stimulating insulin secretagogue to promote insulin secretion only when blood glucose is elevated. It is also known to improve insulin secretion as well as to improve insulin action.

Another drug is the acarbose family of drugs that interferes with the digestion and absorption of carbohydrates and prevents elevated blood sugar after meals. Acarbose is a drug that reversibly inhibits α -glucoamylase activity and is known to inhibit the activity of maltase in the mucosa of small intestine cells [Carrascosa JM, Molero JC, Fermin Y, Martinez C, Andres] A, Satrustegui, J. 2001. Effects of chronic treatment with acarbose on glucose and lipid metabolism in obese diabetic Wistar rats. Diabetes Obes Metab 3: 240-248].

In addition, there are drugs of the pioglitazone line that enhance insulin action, and there are metformin lines that reduce your biosynthesis in the liver.

In addition, in Korea and in Asia, various medicinal herbs have been used for the treatment of diabetes and various diseases as folk remedies. Krenisky et al. Reported the separation of bakuchiol from the Peruvian traditional plant, Otholobium pubescens , as a substance involved in insulin sensitivity [Krenisky JM, Luo J, Carney JR. Isolation and antihyperglycemic activity of bakuchiol from Otholobium pubsecens (Fabaceae), a peruvian medicinal plant used for the treatment of diabetes. Biol Pharm Bull 22: 1137-1140], Hong et al. Reported that a mixed prescription consisting of ginseng, astronomical dong, gold, phalanges, baekbaek and munmundong increased glucose uptake in 3T3-L1 adipocytes [Hong SJ, Fong JC. Hwang JH. 2000. Effect of crude drugs on glucose uptake in 3T3-L1 adipocyte. Gaxiong Yi Xue Ke Xue Za Zhi 16: 445-451].

To date, known to be effective in diabetes, Hubak (Korea Patent Publication No. 2003-0039241), Ginseng leaves (Korea Patent Publication No. 2005-0054905), Hwangyeon (Korea Patent Publication No. 2004-0014556) have. In addition, herbal medicines that have an insulin-sensitive effect that enhances the action of insulin, an insulin-like effect that acts like insulin, and an α -glucoamylase inhibitory effect that interferes with the digestion and absorption of carbohydrates include Yulmu, Morus bark, Roots, and Schisandra chinensis. Patent 2004-0016145) and fractions of Mokdanpi (Korean Patent Publication No. 2003-0100558) have already been found to contain insulin sensitive substances. In particular, the extract of Rhubarb Root showed the effect of lowering blood glucose by increasing glucose utilization in the body by improving insulin sensitivity in 90% of the pancreas from which the pancreas was removed [Choi SB, Park S. 2002. The effects of water extract of Polygonatum Odoratum ( Mill) Druce on insulin resistance in 90% pancreatectomized rats. J Food Sci 67: 2375-2379. It has also been shown that steroidal glycosides contained in the roots act as insulin-sensitive substances in 90% of pancreas-removed white papers [Choi BS, Park S. 2002. A Steroidal Glycoside from Polygonatum odoratum (Mill. ) Druce. Improves Insulin Resistance but does not Alter Insulin Secretion in 90% Pancreatectomized Rats. Biosci Biotech Biochem 66: 2036-2043.

However, for many herbs that are used as folk remedies, the effect is still not clear.

Accordingly, the present inventors have found a herbal medicine that improves insulin secretion by glucose stimulation in beta cells and increases GLP-1 secretion in colorectal cancer cells among various herbal medicines, and the mixed extract including such herbal medicines has a hypoglycemic effect in vivo. And increase insulin secretion, increase insulin action and decrease insulin resistance in vivo, increase insulin secretion in vivo, and increase expression of IRS2 in island of Langerhans, thus preventing and treating diabetes. The present invention was completed.

The present invention is to provide a composition for preventing and treating diabetes and health food for preventing and improving diabetes comprising a mixed extract of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge.

In order to achieve the above object, the present invention provides a composition for preventing and treating diabetes comprising a mixed extract of gold, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade.

In addition, the present invention provides a health food for preventing and improving diabetes comprising a mixed extract of gold, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade porridge.

The mixed extracts of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention showed a hypoglycemic effect and an insulin secretion increasing effect in vivo, and an effect of increasing insulin action and decreasing insulin resistance in vivo. In particular, it increased insulin secretion in vivo, increased expression of IRS2 in the island of Langerhans, and showed no acute toxicity in cells and animals. Therefore, the composition of the present invention comprising a mixed extract of gold, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade according to the present invention can be usefully used as a composition for preventing and treating diabetes.

Hereinafter, the present invention will be described in detail.

Golden ( Scutellaria baicalensis ) is a perennial herb used to dry golden roots. The roots of gold contain baicalein, baicalin, wogonin and neobaiicalein. Physiological activity and pharmacological action of gold, antipyretic, anti-inflammatory, anti-allergic action, and expands blood vessels, and further lowers blood pressure and inhibits atherosclerosis. In particular, Baikalin contained in gold is sedating and hemostasis (止血) because it reduces the permeability of capillaries. In addition, by strengthening the mast cell membrane to suppress the release of chemical transport material, it has anti-allergic effect, in particular to improve hepatitis caused by allergies and inhibit vascular permeability (Jung Bo-seop and Shin, Min-Kyo; Hyangjedae, Yeonglimsa, pp864- 865, 1998), anti-inflammatory and hyperlipidemic effects (Uchiyama et al., J. Med. Pharm. Soc. Wakan-Yaku, 6, 158-164, 1989; Kubo et al., Chem. Pharm Bull., 32, 2724-2729, 1984).

Coptis chinensis is a perennial herbaceous plant belonging to the family Ranuculaceae , and its roots are dried in the sun. The roots of nasturtium contain alkaloids such as berberine, coptisine, warenine, and palmatine as active ingredients. Lactobacillus has antimicrobial action and anti-pathogenic action, especially berberine enhances the action of acetylcholine, relaxes vascular smooth muscle (Jung Bo-seop and Shin Min-kyo; Yaksaimsa, Yeonglimsa, pp490-493, 1998), anti-inflammatory Action (Ko et al., Eur. J. Pharmacol., 399, 187-196, 2000), antioxidant inhibitory activity (Yokozawa et al., J. Pharm. Pharmacol., 56, 547-556, 2004) and lowering blood pressure It is known to be effective.

Hwangbaek ( Phellodendron amurensis ) is a term that refers to the bark of the yellow bark as a medicinal herb, also known as hwanggyeongpi . It is a deciduous broad-leaved arboreous tree, growing up to 10m in height. The leaves are opposite each other. The pinnate leaves are 5 ~ 13 sheets long, egg-shaped lanceolate or long round. The leaf edge has small jagged and soft hairs, and the base of the middle vein has some long hairs. Yellow flowers bloom in May-June, which is a monolith of the male sex family. The cone-shaped stamen is a long circle, with five stamens in the male flower, and the degenerated stamens in the female flower are scaly. It has 5 rooms, with short pistils and 5 pistils. The fruit has a lot of berry and has a hard core in the center, and when rounded, it is purple-black. In Korea, it is distributed in all regions except Jeju and Jeonnam, and is distributed in Japan, Manchuria, China, Amur, and Ussuri, and grows in scrub forests or valleys in the mountains. Peel off the bark and remove the skin (조: rough bark) or cut and dried in the sun, this is the white pill is used as a medicine to lower blood sugar. In addition, pneumococcal pneumoniae, pneumococcal tuberculosis, staphylococcus, etc., while inhibiting the development of tumor cells, and inhibits the growth and sterilization. When taken, palpation of gastric juice is promoted by palpation of taste reflexes, which also leads to an increase in appetite. In addition, since the general alkaloids do not have a systemic effect, even if administered in large amounts, there are no side effects, so it can be used as a formal agent as well as a stomach agent. In addition, since the resistance of various bacteria to this medicine does not occur, it can be used as a face wash disinfectant when a pandemic eye disease is epidemic. In addition, blood pressure lowering, central nervous system inhibition, anti-inflammatory effects have been reported, and is used in oriental medicine such as Hwangnyeonhaedoktang, Shihocheonggantang, Hyeonggaeyeongyotang.

Gardenia ( Fructus ) is the fruit of Gardenia jasmoides Ellis or other cohorts belonging to the Rubiaceae family, and has been used as a food coloring agent since ancient times. In oriental medicine, it is used for anti-inflammatory, diuretic, hemostatic, antipyretic, soothing, antibacterial, bile secretion, hepatitis, jaundice, hemostasis, and is applied as a sedative. The pharmacological action of gardenia was reported to promote bile secretion and protect against drug-induced hepatic disorders (Yamauchi et al. Plant ed. 1974, 25, 219, 285; Journal of Pharmacognosy et al. 1994, 25, 368), serum trigly Decreases triglycerides, phospholipids, lipid peroxides, blood sugar and free fatty acids (Kimura et al. Chem. Pharm. Bull. 1982, 30, 4444), suppresses gastric motility and decreases tension (Aburada et al. J. Pharm. Dyn. 1980, 3, 423), suppresses the increase in the amount of bilirubin in the blood, and has been reported to be effective in preventing atherosclerosis (Gainer et al. Experimentia 1975, 31, 548; Nishizawa et al. Chem. Pharm. Bull. 1987, 35, 2133).

Schizandrae Fructus is a deciduous broad-leaved vine, leaves alternate, ovate or obovate, with acutely pointed, jagged, and somewhat hairy on the back vein. Flower is reddish white and blooms in June ~ July. Fruits are used for food and hang on the ends of branches. They ripen red in August-September. Fruits are called Schisandra (Fructus) and Schizandrae Fructus. 200 ~ 1,600m above sea level Jirisan, Jeonbuk, and Gangwon-do are the main production areas, and are wild throughout the country except Chungnam and Chungbuk, and geographically distributed in Japan, Sakhalin, Manchuria, and China. Fruits include chizandrol, chizandrin A, B, C, angeloylgomisin O, P, epigomisin, pregomoisin, deoxyschizandrin ), And the like. Schisandra chinensis has long been used in herbal medicine as a raw material of medicinal herbs and has the effects of astringent, nourishment, tonic, ginhae medicine, haejudok, thirsty, convergent shovel, ripe ginjin, bosin dyed heart. Human stability with ingestion has already been confirmed.

Jadeok ( Polygonatum Officinale ) is medicinal as the root of the round, medicinal tinnitus (異 名) is called as Jijeol (地 節), Hwangji (黄 地), skinny (女 委) and so on. Dungle, the origin of jade, is a perennial herb. The root of this plant, jade, is harvested in the fall or early spring to remove stems and beard roots, dried in the shade, or steamed and sun-dried in the sun. This jade contains components such as steroidal saponin (convallamarin) and convallarin (steroidal saponin) and a number of flavonoids (flavonoids), cardiovascular action, corticosteroid-like action, It is known to have a thirst quenching action, antioxidant action, and skin aging action. In oriental medicine, the properties of this medicine are flat and sensation, nourishment, lubrication, and jigal, and it is effective for weak constitution, bungalow, diabetes, angina pain. Take it as a pill or powder.

The preparation process of the extract according to the present invention is as follows.

Golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge are washed to remove foreign substances, and then dried in the shade. Dried golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis, Jade porridge or these are mixed and ground in a suitable ratio, preferably in a weight ratio of 1: 1: 1: 1: 2: 2. Appropriate amount of solvent is added to the pulverized golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis, jade porridge or mixtures thereof so as to be completely immersed. The extraction solvent is preferably a solvent selected from water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, more preferably water. The amount of the extraction solvent is preferably 5 to 20 times, preferably 10 times, the dry weight of golden, yellowish white, gardenia, yellow lotus, schizandra, jade porridge or mixtures thereof. The extraction method can be impregnated at room temperature or warmed, preferably hot water extraction. The prepared golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis, jade porridge or a mixed extract thereof may be used through filtration, concentration and / or lyophilization.

Oral administration of glucose compared to the control group in the 90% pancreas-removed rat group showing type 2 diabetes mellitus administered with a mixed extract (extract B) of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention for 2 months The serum glucose level was significantly lower, indicating that the effect of lowering blood glucose was excellent, and insulin secretion was also increased (see 3a and 3b). At this time, the extract A is a mixed extract of golden, yellow white, gardenia and yellow lotus, extract B is a mixed extract of golden, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade.

In addition, in the hyperinsulinemic euglycemic clamp experiment for measuring insulin resistance in vivo, the group administered with extract B showed a significant increase in glucose infusion rate and increased insulin sensitivity (see FIG. 4A). It was found that the glucose production rate in the liver in the high insulin state was effectively decreased (see FIG. 4B), and the serum insulin concentration in the hyperglycemic state was also increased in the first and second stages. It can be seen that the control group and other groups appear higher (see FIGS. 5A and 5B).

In addition, extract B induces the expression of IRS2 in the Langerhans islet, the first signal of insulin / IGF-1 signaling (see FIG. 6), thereby increasing the function and viability of beta cells to enhance blood glucose lowering action. there was.

Since the above effects were superior to the use of gold, yellowish white, gardenia, yellow lotus, Schisandra chinensis, and jade extract alone, the extract according to the present invention had an unexpected and unexpected effect different from the results expected due to the mixing of the herbal medicines. Appeared. That is, the Schizandra chinensis extract did not increase insulin secretion as shown in FIGS. 3b and 5a, and did not increase the expression of IRS2 as shown in FIG. 6. When mixed with and obtained a new herbal mixed extract according to the present invention, the extract B shows an excellent effect than the conventional extract of Schizandra chinensis or the herbal extracts consisting of golden, yellow, white, gardenia and yellow lotus in the insulin secretion and insulin action enhancement activity Confirmed.

Extract according to the present invention is considered to be a safe substance as a mixed extract of herbal medicines that have been used in the past.

The composition of the present invention can be usefully used for the treatment and prevention of diabetes, the diabetes is characterized by type 2 diabetes.

Therefore, the mixed extracts of the golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge of the present invention exhibits an effective hypoglycemic action and insulin secretion action, decreases insulin resistance in vivo and increases the expression of IRS2, thus containing the extract. The composition of the present invention can be usefully used as a composition for preventing and treating diabetes.

The composition of the present invention may contain one or more active ingredients exhibiting the same or similar functions in addition to the mixed extract of gold, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade.

The composition of the present invention can be prepared by including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients for administration. Pharmaceutically acceptable carriers may be used in combination with saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components, if necessary, as antioxidants, buffers Other conventional additives such as bacteriostatic agents can be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Furthermore, it may be preferably formulated according to each disease or component by a suitable method in the art or using a method disclosed in Remington's Pharmaceutical Science (Mack Publishing Company, Easton PA, 18th, 1990).

The compositions of the present invention may be administered parenterally (eg, intravenously, subcutaneously, intraperitoneally or topically) or orally, depending on the desired method, and the dosage may be based on the weight, age, sex, health status, The range varies depending on the diet, the time of administration, the method of administration, the rate of excretion and the severity of the disease. The daily dose is 10 to 500 mg / kg, preferably 100 mg / kg, in an amount of lyophilized extract according to the present invention, preferably administered once to several times a day.

The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy and biological response modifiers for the prevention and treatment of diabetes.

The composition of the present invention can be added to health food for the purpose of preventing and improving diabetes. When using the mixed extract of the golden, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention as a food additive, the mixed extract of the golden, yellow white, gardenia, yellow lotus, schisandra chinensis and jade porridge is added as it is or with other food or food ingredients It can be used together and can be appropriately used according to a conventional method. The blending amount of the active ingredient can be suitably determined according to the purpose of its use (prevention, health or therapeutic treatment). However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. Is sure.

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and the like and include all of the health foods in the conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The proportion of said natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, And a carbonation agent used for the carbonated beverage. In addition, the composition of the present invention may contain a flesh for preparing natural fruit juice, fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

EXAMPLES Preparation of Mixed Extracts of Gold, Yellowish White, Gardenia, Rhododendron, Schisandra chinensis and Jade Rice

Golden, yellow white, gardenia, yellow lotus, schizandra and jade porridge were washed and dried in the shade. Dried Mixture A (Golden: Yellow White: Gardenia: Ranum = 1: 1: 1: 1 Weight Ratio), Mixture B (Golden: Yellow White: Gardenia: Russium: Schisandra: Jeju = 1: 1: 1: 1: 2: 2 Weight Ratio ), Schisandra chinensis and Gold were ground each 1.2 kg. 8 L of distilled water was added to the pulverized material, and extracted using an ultrasonic mixer (Sony Medi, Korea) for 70 minutes at 90 ° C., and filtered through a filter twice (No 2. filter) after refrigeration for 12 hours. The filtrate was centrifuged at 450 × g (Megafuge 1.0R, USA) to recover the supernatant, which was concentrated under reduced pressure at 35 ° C. with a rotary vacuum concentrator (Buchi R-114, USA) and freeze-dried (Ilshin Freeze dryer, Korea) to prepare each extract in powder form. At this time, the yield was 8%.

The powder produced by concentrating the extract was dissolved in sterile dimethylsulfoxide (DMSO; Sigma Co., St. Louis, USA) and re-diluted with a culture solution to obtain a final concentration of 50ug / mL.

Experimental Example 1 Measurement of GLP-1 Secretion in Colorectal Cancer Cell Lines

1. Cell Culture

The NCI-H716 cell line used for the experiment was purchased from the American Cell Line Bank (ATCC), 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 IU / ml penicillin, 100 Suspension cells were cultured in Roswell Park Memorial Institulete (RPMI) medium containing μg / ml of streptomycin and the like. Cultured cells were transferred to dishes coated with Matrigel (Becton Dickinson, Bedford, Mass., USA) and used for GLP-1 secretion experiments.

Two days before the experiment, 1 x 10 ⁶ cells / ml of differentiated cells were transferred to Matrigel coated 12-well culture plates, followed by 10% FBS, 2 mM el-glutamine, 100 IU / ml penicillin, The cells were cultured in RPMI medium to which 100 µg / ml of streptomycin and the like were added.

2. GLP-1 Secretion Measurement Experiment

The culture medium of the plate in which the differentiated cells were cultured was prepared with KRB buffer solution containing 0.2% (w / v) bovine serum albumin (BSA) and extracts of each of golden, yellow, white, gardenia, barberry, schisandra chinensis and jade. bicarbonate, pH 7.2) and incubated for 2 hours. Collect KRB solution from each well and add it to -80 ° C until 50 μg / ml of phenylmethylsulphonyl fluoride (PMSF) and 34 μg / ml of diprotin-A were added to measure GLP-1 concentration. Stored in.

The cells in the wells were scraped and scraped with 1 M hydrochloric acid (HCl), with 1% (v / v) trifluoroacetic acid, including homogenization buffer (5% (v / v) formic acid (HCOOH). ) And 1% (w / v) sodium chloride) and the digested intracellular peptides were extracted by alcohol extraction method.

Radioimmunoassay using the Linco GLP-1 Kit (GLP-1 (736) Active RIA Kit, Linco Research Inc., St. Charles) for the release of GLP-1 in KRB solution and the amount of GLP-1 in cells In order to correct the amount of cells, the protein amount of the cell homogenate was quantified by the Bradford protein assay (Bio-Rad, Inc.).

3. Effect of each extract of herbal medicine on GLP-1 secretion of NCI-H716 colorectal cancer cells

As a result of measuring the secretion of GLP-1 in the NCI-H716 cell line, among the extracts of gold, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade, the gold and yellow white increased GLP-1 secretion (see FIG. 1A).

All results were expressed as mean ± standard deviation (SD), and ^* in the figure indicated that the one-way analyses of variance (ANOVA) showed a remarkable difference from other groups at P <0.05 level. ^{The a and b} values are P <0.05 by Tukey's test, and the same terms It showed a difference. Statistical analysis in all the following Experimental Examples were as described above.

Experimental Example 2 Insulin Secretion Measurement in Pancreatic Beta Cell Line

1. Cell Culture

The Min6 cell line used in the experiment was purchased from the American Cell Line Bank and contained high concentrations of DMEM (Dulbecco's) containing 15% FBS, 2 mM el-glutamine, 100 IU / ml penicillin, and 100 μg / ml streptomycin. cultured in modifiedEagle's medium).

Two days before the experiment, 1 x 10 ⁶ cells / ml of cells were transferred to a 12-well plate, followed by high concentration of 10% FBS, 2 mM el-glutamine, 100 IU / ml of penicillin, and 100 μg / ml of streptomycin. Cultured in glucose DMEM medium, the next day was incubated with a low concentration of glucose DMEM medium containing 0.2% BSA.

2. Insulin Secretion Measurement Experiment

The culture medium of the plate in which the cells were cultured was changed to KRB solution (pH 7.2) containing 0.2% (w / v) BSA and extracts of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade, and incubated for 2 hours. KRB solutions from each well were collected and stored at -80 ° C until insulin concentration was measured.

The cells in the wells were scraped and digested with the grinding buffer, and the peptides in the digested cells were extracted by alcohol extraction.

Radioimmunoassay using Linco Research Inc. (St. Charles) was used to quantify the amount of insulin secreted from the KRB solution and the amount of insulin present in the cells. Quantification by protein quantification.

3. Effect of each extract of herbal medicine on insulin secretion of Min6 cells

As a result of measuring insulin secretion in the Min6 cell line, among the extracts of gold, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade, the gold and yellow white increased insulin secretion (see FIG. 1B).

Experimental Example 3 Preparation of Experimental Animals and Administration of Herbal Extracts

1. Production of experimental animals showing diabetes symptoms

90% of pancreas of 8-week-old male SD rats (Sprague Dawley rats) were removed by Hosokawa et al. To produce experimental animals with mild diabetes (90% pancreatectomized rats). Experimental animal controls (sham operated rats) were sutured after touching the fingers with the pancreas without excision by the same operation as the surgery for pancreas resection.

Two weeks after the operation, the animals were fed with solid feed, and those with poor surgical procedures and those with less than 9.4 mmol / l of blood sugar were excluded from the test subjects. About 40-50% of the diabetic model animals were manufactured. It turned out to be symptomatic.

The experimental animal control group did not show diabetes.

2. Determination of Blood Glucose and Serum Insulin Levels on Fasting and After Meal

In order to measure fasting blood glucose, the animals were fasted from 6 pm to 10 am the following day once a week, and blood was collected from the femoral vein. Plasma of the collected blood was separated and blood glucose was measured by a glucose analyzer (Glucose Analyzer, Beckman Instruments, Fullerton, CA).

Post-prandial blood glucose was measured at 10 am once a week by taking blood from experimental animals given normal diet.

Serum insulin concentrations were quantified by radioimmunoassay using a Rat Insulin Specific RIA kit, Linco Research Inc., St. Charles, USA.

3. Administration of Herbal Extracts to Experimental Animals with Diabetic Symptoms

The following experiment was performed after oral administration of the control group (DMSO), the extract A obtained from the mixture A, the extract B obtained from the mixture B, the golden extract and the Schizandra chinensis extract for 2 months.

The dosage of each extract was 600 mg / kg body weight / day of golden, yellow white, gardenia and yellow lotus in extract A, and 300 mg / kg body weight / day of golden, yellow white, gardenia and yellow lotus in extract B, respectively. It was administered to / kg body weight / day. The gold extract was orally administered to the experimental animals such that 2,400 mg / kg body weight / day and Schisandra chinensis extract was 2,400 mg / kg body weight / day.

4. Changes in dietary intake, body weight and visceral fat content of experimental animals

While administering each composition as described above for 8 weeks, the daily dietary intake was recorded at regular intervals, and the body weight and visceral fat level after completion of the administration were measured.

Daily dietary intake, body weight and visceral fat were not significantly different in the control group, extract A treatment group, extract B treatment group, golden treatment group and Schizandra treatment group. It seems that the results of the daily dietary intake were reflected in the body weight and visceral fat (epidydimal fat pads) (see Figure 2).

<Experiment 4> Effects of herbal extracts on hypoglycemic effect and insulin secretion in vivo

The control animals (DMSO), extract A, extract B, golden extract, Schisandra chinensis extract were administered to experimental animals showing type 2 diabetes symptoms for 2 months.

In order to perform the oral glucose tolerance test (OGTT) on these test animals, glucose and insulin secretion were measured after oral administration of 2 g of glucose per kg of body weight. Thereafter, after 0, 10, 20, 30, 45, 60, 90 and 120 minutes, blood was collected from the tail to measure blood glucose levels, and the area of the lower portion of the curve was calculated. ^* Was significantly different from other groups with P <0.05, and ^{a, b, c} of the rods by Tukey test The value is P <0.05, which is remarkable Showed a difference.

The concentration of glucose in serum is shown in FIG. 3A, and the AU (Artificial Unit) of glucose and insulin in serum is calculated and shown in FIG. 3B. At this time, AU means the average concentration for 1 minute from the time which shows the highest concentration in serum. AU of FIG. 3b was used as an indicator of the degree of decrease in glucose and increase of insulin secretion in serum.

As shown in the results, the blood glucose levels were low in the group treated with Extract A and Schisandra chinensis extract, but not statistically significant, and only the extract B treated group showed a statistically significant effect of lowering blood glucose compared to the control group (Fig. 3a).

Insulin secretion was significantly higher than the control group in the extract A, extract B, golden extract treatment group (see Figure 3b). In particular, it showed the highest insulin secretion in the group treated with the extract B of the present invention, suggesting that it is related to the lowest blood glucose value in the group treated with the extract B.

Experimental Example 5 Effect of Medicinal Herb Extracts on Insulin's Action and Insulin Resistance

1. Extract Administration and Surgery for Experimental Animals

To measure insulin sensitivity, a hyperinsulinemic euglycemic clamp study was performed. In the same manner as in <Experiment 3>, the following experiment was performed after oral administration of extract A, extract B, golden extract and Schizandra extract for 2 months to rats of each group.

A catheter (PE-10, Intramedic, Clay Adams, Parsippany, NJ) was inserted into the jugular vein on the right side and the carotid artery on the left side for substance administration and blood collection. After 1 week of recovery.

2. Hyperinsulinemia Normal Glucose Clamp Experiment to Measure Insulin Resistance

The recovered rats were infused with labeled glucose ([3- ³ H] glucose) at a rate of 0.15 mCi / min for 1 hour, followed by continued infusion of labeled glucose with 12 mU / kg of insulin and 25% glucose solution. Intravenously injected at a rate of / min to maintain a steady-state in which normal blood glucose (euglycemia) is maintained.

Glucose infusion rate (GIR) was determined by collecting blood from the arteries at 5 minute intervals during this period to maintain blood glucose at 90-100 mg / dl. For blood loss during the experiment, blood was collected from other rats and supplemented by intravenous dilution with 1: 1 (v: v) in heparinized saline (10 U / ml).

The glucose infusion rate was expressed as the glucose content injected per minute, per unit weight, and injected per minute by multiplying the glucose concentration of the solution to be injected to the glucose infusion rate required to maintain blood sugar at 90-100 mg / dl (Figure 4a). Reference).

As shown in the results, compared with the control group, the glucose injection rate of the group treated with the extract B and Schisandra chinensis extract statistically significantly increased, especially the group treated with extract B of the present invention showed the highest glucose injection rate.

3. Measurement of glucose specific activity

The specific activity of labeled glucose in serum was measured using Somogi method. The serum protein was precipitated with barium hydroxide (Ba (OH) ₂ ) and lead sulfate (ZnSO ₄ ), and the supernatant was separated and dried at 55 ^° C. to remove ³ H ₂ O. The obtained dried product was dissolved in 0.1 ml of distilled water, and then 3 ml of a radiation solution (scintiverse) was added thereto, and the radioactivity (dpm) was measured by a liquid scintillation counter. The radioactivity (dpm) of ³ H ₂ O in serum was calculated as the difference between the radioactivity measurement value of the undried supernatant after serum protein removal and the radioactivity measurement value after drying the supernatant.

In this case, the glucose uptake rate is a value representing the degree of glucose inflow into the vascular system, and the glucose disposal rate is a value indicating the degree of glucose loss from the vascular system due to the action of insulin. Intake rate and glucose loss rate are the same. Glucose loss rate was calculated as the infusion rate of labeled glucose (dpm / min) divided by the specific activity of labeled glucose (dpm / mg) at steady state, which means the glucose intake rate (see FIG. 4A). .

As described above, the glucose infusion rate showed the highest value in the group treated with Extract B, but the glucose intake rate was not different in the Extract A, Extract B, Golden Extract, and Schisandra extract treated groups as shown in FIG. I can see that. That is, in the group treated with the extract B, the extract of the present invention, the amount of glucose introduced into the blood vessel is constant despite the absolute amount of glucose injected into the experimental animal, and consequently, glucose consumption is increased in organs other than the blood vessel in vivo. It's big.

4. Effect on Hepatic Insulin Resistance

Labeled glucose was injected into the experimental animals subjected to the above surgery at a constant rate for 40 minutes to maintain the specific state of the labeled glucose in serum.

Glucose production rate in the liver in hyperinsulinemia (hyperglycemic normal glucose clamp test) was calculated by subtracting the glucose intake from the outside from the glucose intake into the vasculature and basal glucose production rate in the liver. The production rate in hyperinsulinemia is shown in Figure 4b.

As shown in Figure 4b, when the blood insulin concentration is high, the glucose production rate of the group treated with the extract B and Schizandra extract statistically significantly decreased, especially the lowest glucose production rate in the group treated with the extract B of the present invention Indicated. From these results, it can be seen that the extract B of the present invention effectively reduces insulin resistance in the liver. The fasting state showed the lowest glucose production rate in the group treated with the extract B of the present invention, but was not statistically significant.

Experimental Example 6 Effect of Medicinal Herb Extracts on Insulin Secretion of Pancreatic Beta Cells

To measure insulin secretion, a hyperglycemic clamp test was performed. The catheter was inserted into the jugular vein on the right side and the carotid artery on the left side for the administration of the substance and blood collection, and recovered for 1 week after the surgery. After recovery, the body returned to normal fasting for 12 hours, and then the hyperglycemic clamp test was performed while moving freely.

Blood was collected every 10 minutes while injecting 25% glucose into the experimental animals to adjust the glucose infusion to maintain blood glucose at 230 mg / dL. Serum of 0, 2, 5, 10, 60, 90, 120 minutes of the collected blood was stored at -70 ^o C and serum insulin concentration was measured.

The serum insulin concentration over time is shown in FIG. 5A and the AU of insulin in the first and second stages is shown in FIG. 5B. In this case, the AU of the first stage and the second stage refers to the average concentration for one minute from the time indicating the highest concentration of the first peak and the highest concentration of the second peak in the serum insulin concentration curve, respectively.

As shown in Figure 5a, the concentration of insulin in the serum was high in the group treated with extract B of the present invention.

In addition, as shown in Figure 5b in the first step was statistically significantly increased in the group treated with extract B, and in the second step was significantly increased in the group treated with extract A and extract B. In particular, the group treated with the extract B of the present invention showed the highest insulin concentration value in the first and second stages.

Experimental Example 7 Effects of Medicinal Herb Extracts on the Expression of IRS2 in Pancreatic Beta Cells

The expression level of IRS2 was examined by measuring the expression of glucokinase and GLUT2, which are closely related to beta cell function and whether IRS2 expression was increased in beta cells.

After treatment with each of the extracts on the isolated island of Langerhans and incubated for 8 hours, total RNA was isolated from the island of Langerhans treated with a control or trizol (GIBCO BRL, Life Technologies, Inc., Grand Island, New York). CDNA was synthesized with the RETRO script kit (Ambion Inc., Austin, Texas) using oligo dT on the isolated RNA. And quantitative Realtime PCR [4 minutes at 94 ° C., 30 minutes at 94 ° C., 55 minutes] using iCycler PCR instrument (Bio-Rad Laboratories, Hercules, California) and QuantiTect SYBR Green PCR kit (Qiagen Inc., Valencia, California). 30 seconds at 0 ° C., 30 seconds at 72 ° C.) × n and 5 minutes at 72 ° C.].

Quantitative analysis was performed by calculating the relative ratios of the internal control cyclophilin and the gene PCR product of interest.

The results are shown in FIG.

As shown in Figure 6, the expression of IRS2 was significantly increased in the group treated with Extract A, Extract B and golden extract.

Preparation Example 1 Preparation of Pharmaceutical Formulation

1-1. Manufacture of powder

2g herbal medicine mixed extract according to the present invention

1g lactose

The above components were mixed and packed in airtight bags to prepare powders.

1-2. Manufacture of tablets

100 mg herbal extract extract according to the present invention

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

1-3. Preparation of Capsules

100 mg herbal extract extract according to the present invention

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

1-4. Preparation of Injection

10 ㎍ / ㎖ herbal medicine extract

Dilute hydrochloric acid BP until pH 3.5

Injectable sodium chloride BP up to 1 ml

Dissolve the herbal mixture extract according to the present invention in a suitable volume of injectable sodium chloride BP, adjust the pH of the resulting solution to pH 3.5 with dilute hydrochloric acid BP, adjust the volume with injectable sodium chloride BP and mix well It was. The solution was filled into a 5 ml Type I ampoule made of clear glass, encapsulated under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120 ° C. for at least 15 minutes to prepare an injection solution.

Preparation Example 2 Preparation of Food

Food products containing a mixed extract of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention were prepared as follows.

2-1. Manufacture of flour food products

Add 0.5 ~ 5.0% by weight of the mixed extract of golden, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention to flour, and prepare bread, cake, cookies, crackers and noodles using this mixture to improve health Was prepared.

2-2. Preparation of soups and gravy

0.1 to 1.0 parts by weight of the mixed extracts of golden, yellow and white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention were added to soups and broth, respectively, to prepare meat products for health promotion, soups and broths in a conventional manner.

2-3. Preparation of Ground Beef

10 parts by weight of the mixed extracts of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade in accordance with the present invention were added to ground beef to prepare ground beef for health promotion in a conventional manner.

2-4. Manufacture of dairy products

5-10% by weight of the mixed extract of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

2-5. Manufacture of wire

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted to prepare a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The extract of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention was concentrated under reduced pressure in a vacuum concentrator, and dried by spraying and drying with a hot air dryer to pulverize the dried product to a particle size of 60 mesh to obtain a dry powder.

The dry powders of the grains, seeds and the extracts of the golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porcine according to the present invention were prepared by blending in the following ratios.

Cereals (30% by weight brown rice, 15% by weight radish, 20% by weight barley),

Seeds (7% by weight perilla, 8% by weight black beans, 7% by weight black sesame),

Dry powder (3% by weight) of the mixed extract of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention,

Ganoderma lucidum (0.5% by weight),

Foxglove (0.5 wt%)

Preparation Example 3 Preparation of Beverage

3-1. Preparation of Carbonated Drinks

5-10% of sugar, 0.05-0.3% citric acid, 0.005-0.02% caramel, 0.1-1% of vitamin C are mixed, and 79-94% purified water is mixed to make syrup, and the syrup is 85-98 Sterilizing at 20 ℃ for 180 seconds and mixed with a cooling water at a ratio of 1: 4, and then inject 0.5 ~ 0.82% of carbon dioxide gas, containing a mixed extract of gold, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade according to the present invention Carbonated drinks were prepared.

3-2. Manufacture of health drinks

Of subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%), and gold, yellowish white, gardenia, yellow lotus, schizandra and jade porridge according to the present invention. The mixed extract was homogeneously blended and sterilized immediately and then packaged in a small packaging container such as a glass bottle or a plastic bottle to prepare a healthy beverage.

3-3. Preparation of Vegetable Juice

5 g of a mixed extract of golden, yellowish white, gardenia, yellow lotus, Schisandra chinensis and jade porridge according to the present invention was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice for health promotion.

3-4. Preparation of Fruit Juice

1 g of a mixed extract of golden, yellow and white, gardenia, yellow lotus, Schisandra chinensis and jade in accordance with the present invention was added to 1,000 ml of apple or grape juice to prepare a fruit juice for health promotion.

Figure 1a is a diagram showing the effect of GLP-1 secretion of NCI-H716 colorectal cancer cells extract of gold, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade porridge.

Figure 1b is a diagram showing the effect of each extract of gold, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade porridge on insulin secretion of Min6 beta cells.

Figure 2 is a diagram showing the daily dietary intake, body weight and degree of epididymal fat pad of the control animals, extract A, extract B, golden extract and Schizandra extract.

Figure 3a is a diagram showing the concentration of glucose in serum with time after administration of glucose to the experimental animal groups to which the control group, extract A, extract B, golden extract and Schizandra extract.

Figure 3b is a diagram showing the AU of glucose and insulin concentrations in the serum group of the control group, extract A, extract B, golden extract and Schizandra extract (wherein AU (Artificial Unit) represents the highest concentration in the serum) Mean concentration for 1 minute from time, and extract A is a mixed extract of golden, yellow white, gardenia and yellow lotus, and extract B is a mixed extract of golden, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade.)

Figure 4a is a diagram showing the glucose infusion rate and glucose intake rate of the group administered the control group, extract A, extract B, golden extract and Schizandra extract.

Figure 4b is a diagram showing the baseline glucose production rate in the liver of the control group, extract A, extract B, golden extract and Schisandra chinensis extract and glucose production rate in high insulin state.

Figure 5a is a diagram showing the concentration of insulin in the serum after administration of glucose for the group administered the control group, extract A, extract B, golden extract and Schizandra extract.

Figure 5b is a diagram showing the AU of the first step and the second step of the group administered the control group, extract A, extract B, golden extract and Schizandra extract.

Figure 6 is a diagram showing the expression level of IRS2 in the group administered with the control, extract A, extract B, golden extract and Schizandra extract.

Claims (10)

A composition for preventing and treating diabetes comprising a mixed extract of golden, yellowish white, gardenia, yellow lotus, schisandra chinensis and jade porridge The method of claim 1, wherein the mixed extract is a composition for preventing and treating diabetes, characterized in that extracted by mixing in a weight ratio of 1: 1: 1: 1: 2: 2 of gold, yellow white, gardenia, yellow lotus, Schisandra chinensis. The method for preventing and treating diabetes of claim 1, wherein the mixed extract is extracted with a solvent that is water, alcohol or a mixture thereof. According to claim 3, wherein the alcohol is a lower alcohol of C ₁ to C ₄ composition for the prevention and treatment of diabetes. 5. The composition for preventing and treating diabetes of claim 4, wherein the lower alcohol is ethanol. The method of claim 3, wherein the mixed extract is a composition for preventing and treating diabetes, characterized in that extracted at 1 ~ 24 hours conditions at 50 ~ 90 ℃. [Claim 2] The composition for preventing and treating diabetes of claim 1, wherein the mixed extract has insulin secretion enhancing or insulin action enhancing activity. 2. The composition for preventing and treating diabetes of claim 1, wherein the diabetes is type 2 diabetes. Health food for the prevention and improvement of diabetes comprising a mixed extract of golden, yellow white, gardenia, yellow lotus, Schisandra chinensis and jade porridge. 10. The method for preventing and improving diabetes of claim 9, wherein the mixed extract is extracted by mixing golden, yellow and white, gardenia, yellow lotus, Schisandra chinensis and jade porridge in a weight ratio of 1: 1: 1: 1: 2: 2. .

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