KR102203657B1 - A composition comprising the complex extract for antiobesity of women - Google Patents

Abstract

The present invention relates to a composition for anti-obesity containing a complex extract, and more specifically, when ingesting a complex extract of Ogapi stem, arrowroot root, dulcis stem, bokryeong, deodeok root and jujube fruit, body fat mass and abdominal fat percentage are significant. Since it was confirmed that the effect of reducing internally, inhibiting the accumulation of visceral fat and subcutaneous fat, and reducing blood cholesterol, the composite extract of the present invention is used as a composition or functional material for preventing or treating metabolic diseases including obesity. It can be useful.

Description

A composition comprising the complex extract for antiobesity of women}

The present invention relates to a composition for anti-obesity containing a complex extract, more specifically for preventing or treating metabolic diseases including obesity containing a complex extract of Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube as an active ingredient It relates to the composition.

Metabolic diseases include impaired glucose tolerance due to chronic metabolic disorders (pre-diabetes, fasting blood sugar higher than 100mg/dL, conditions that can be restored to normal by proper diet and exercise therapy), hypertension, hyperlipidemia, obesity, It consists of various diseases such as atherosclerosis of the cardiovascular system.

In particular, in women, obesity increases the risk of cardiovascular disease and sleep apnea, such as high blood pressure, insulin resistance, dyslipidemia, and an increase in chronic inflammatory markers, as in men. In addition, in women, diseases related to reproductive or some hormone-dependent tumors related to pregnancy and childbirth, such as polycystic ovary syndrome related to ovulation disorder and infertility, endometrial cancer, breast cancer, colon cancer, and kidney cancer. It is known to increase the risk of tumors. According to the 2008 National Health and Nutrition Survey, the prevalence of obesity with a body mass index of 25 kg/m ² or higher was 14.1% for women in their 20s, 17% for women in their 30s, and 27.7% for women in their 40s. The prevalence of obesity was significantly lower than that of men, but unlike men, the prevalence of obesity was found to increase rapidly after their 40s.

Among obesity, there are people with normal weight but metabolic obesity, and their common feature is that they are abdominal obesity with a stomach out. In other words, even if the weight is normal, the risk of developing diseases such as hypertension, diabetes, and dyslipidemia increases when the belly comes out. Such abdominal obesity usually occurs in people with insufficient activity, and mainly due to an increase in visceral fat mass, abdominal obesity often occurs. In addition, since abdominal obesity patients often have little muscle mass, it is important to maintain muscle mass by exercising concurrently.

On the other hand, in a complex modern society, modern people who are exposed to deterioration of the living environment due to environmental pollution, increased mental stress, lack of activity, etc. are increasingly interested in health promotion. Exercise is the most effective and economical as a preventive measure for adult diseases or aging, but modern people who cannot manage health due to busy daily life and fatigue are interested in taking various functional foods as an alternative to exercise. However, the misuse of functional foods and temporary excessive exercise are at risk of having fatal side effects to health. Accordingly, in recent years, there is a need for the development of functional supplements using natural products with guaranteed safety such as plant extracts.

Accordingly, the present inventors have endeavored to develop a material that can improve obesity, especially abdominal obesity, and prevent side effects caused by indiscriminate diet, excessive exercise therapy, and misuse of drugs, and as a result, Ogapi stems, arrowroot roots, dulcis stems, bokryeong , Deodeok root and hot water complex extract prepared by mixing jujube fruit significantly reduces body fat mass and abdominal fat percentage, inhibits the accumulation of visceral fat and subcutaneous fat, and reduces blood cholesterol. By confirming the appearance, the present invention was completed by revealing that the complex extract of the present invention can be used as a composition or functional material for preventing or treating metabolic diseases including obesity.

Republic of Korea Patent Publication No. 2015-0063183

Seunghyun Lee, Youngchae Jo. 2011. Assessment of obesity based on BMI and body fat percentage of health examination examinees and their relationship with coronary artery disease risk factors. Journal of the Korean Industrial-Academic Technology Society 12: 746-752. Lee Seung-gyo, Won Hyang-rye, and Jung Eun-hee. Life Cycle Nutrition p.220 (2013) Life Science. Yeon-sook Lee, Jae-ok Koo, Hyun-sook Lim, Young-hee Kang, and Jong-suk Kwon. Easy-to-understand human physiology p.37 (2016) Powerbook. Seong Yuna. 2010. Absence of obesity in women. Korean Journal of Internal Medicine 79(2): S471-472.

It is an object of the present invention to provide a pharmaceutical composition for preventing or treating metabolic diseases, containing as an active ingredient a complex extract of Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube.

Another object of the present invention is to provide a health functional food for preventing or improving metabolic diseases, containing as an active ingredient a complex extract of Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube.

In order to achieve the object of the present invention, the present invention provides a pharmaceutical composition for preventing or treating metabolic diseases, containing as an active ingredient a complex extract of Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube.

In addition, the present invention provides a health functional food for preventing or improving metabolic diseases, containing as an active ingredient a complex extract of Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube.

In the present invention, a hot water complex extract was prepared by blending a cucumber stem, arrowroot root, dulcis stem, bokryeong, deodeok root and jujube fruit, and the effect of significantly reducing body fat mass and abdominal fat percentage when ingesting the hot water complex extract , Since it was confirmed that the effect of inhibiting the accumulation of visceral fat and subcutaneous fat and the effect of reducing blood cholesterol is shown, the composition containing the complex extract of the present invention as an active ingredient is a composition or functional composition for preventing or treating metabolic diseases including obesity. It can be used as a material.

1 is a diagram showing the size of adipocytes of each experimental animal group prepared in an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for preventing or treating metabolic diseases, containing as an active ingredient a complex extract of Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube.

The complex extracts of the active ingredients of the present invention, Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube are preferably prepared by a method comprising the following steps, but are not limited thereto:

1) extracting by mixing Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube, and adding water including purified water;

2) filtering the extract of step 1);

3) concentrating the filtrate of step 2) under reduced pressure; And

4) drying the concentrate of step 3).

In the manufacturing method of the present invention, in the step 1), the Ogapi, Arrowroot, Hutgae, Bokryeong, Deodeok and Jujube may be grown or commercially available without limitation. In addition, the Ogapi, Arrowroot, Hutgae Tree, Deodeok and Jujube may use one or more portions selected from the group consisting of leaves, stems, roots and fruits thereof, and more specifically Ogapi stem, Arrowroot, Hutgae tree trunk, Deodeok Roots and jujube fruits can be used.

In addition, in step 1), 20 to 40 parts by weight of Ogapi, 15 to 35 parts by weight of arrowroot, 10 to 30 parts by weight of dulcis, 1 to 20 parts by weight of Bokryeong, 1 to 20 parts by weight of Deodeok, and jujube It is preferable to blend in 0.5 to 15 parts by weight, and 25 to 35 parts by weight of Ogapi, 20 to 30 parts by weight of arrowroot, 15 to 25 parts by weight of walnut trees, 5 to 15 parts by weight of Bokryeong, 5 to 15 parts by weight of Deodeok It is more preferable to mix 1 to 10 parts by weight of a part by weight, and jujube, and 28 to 32 parts by weight of Ogapi, 23 to 27 parts by weight of arrowroot, 18 to 22 parts by weight of birch, 8 to 12 parts by weight of Bokryeong It is more preferable to mix 8 to 12 parts by weight of Deodeok, and 3 to 7 parts by weight of jujube, and 30 parts by weight of Ogapi, 25 parts by weight of arrowroot, 20 parts by weight of halter tree, 10 parts by weight of Bokryeong, It is most preferable to mix 10 parts by weight of deodeok and 5 parts by weight of jujube, but is not limited thereto.

In the production method of the present invention, it is preferable to use water or alcohol as the extraction solvent in step 1), and more preferably, water is used. It is preferable to use a C ₁ to C ₄ lower alcohol as the alcohol, and it is preferable to use ethanol or methanol as the lower alcohol. The extraction method is preferably shaking extraction, Soxhelt extraction, or reflux extraction, but is not limited thereto. It is preferable to extract by adding 1 to 10 times the weight of the mixture to the extraction solvent, and more preferably to extract by adding 2 to 3 times. The extraction temperature is preferably 90 to 130 °C, more preferably 95 to 125 °C, but is not limited thereto. Further, the extraction time is preferably 1 to 48 hours, more preferably 2 to 10 hours, and even more preferably 2 to 8 hours, but is not limited thereto.

In the manufacturing method of the present invention, the vacuum concentration in step 3) is preferably a vacuum vacuum concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, the drying is preferably vacuum drying, vacuum drying, boiling drying, spray drying, or freeze drying, but is not limited thereto.

In the preparation method of the present invention, steps 3) and 4) may be omitted depending on the use of the composite extract of the present invention.

In the present invention, the metabolic disease may be obesity, hyperlipidemia or hypercholesterolosis, but is not limited thereto.

In addition, the obesity may be specifically abdominal obesity, and the abdominal obesity may be subcutaneous fat type abdominal obesity, visceral fat type abdominal obesity or abdominal muscle separating type abdominal obesity, and more specifically, subcutaneous fat type abdominal obesity or visceral fat type. It could be abdominal obesity.

The term "obesity" refers to a state in which adipose tissue is excessive in the body. At the time of diagnosis, if the body mass index (body mass index: weight (kg) divided by the square of height (m)) is 25 or more, it is defined as obesity. More specifically, in Lee and Cho, if the BMI is less than 25 and the body fat percentage is less than 20%, it is "normal", and if the BMI is 25 or more and the body fat percentage is less than 20%, it looks fat, but the body fat is in the normal range of "appearance obesity" and BMI is If the body fat percentage is less than 25 and more than 20%, it is classified as "obesity". Such obesity increases the likelihood of diabetes and hyperlipidemia, and increases the risk of sexual dysfunction, arthritis, cholelithiasis, and cardiovascular disease.

In addition, the term "abdominal obesity" refers to a case in which fat is excessively accumulated in the internal organs of the abdomen, unlike obesity due to subcutaneous fat. The fat in the abdomen is closer to the liver than the fat distributed elsewhere, so it is likely to have a systemic effect. In addition, abdominal obesity has a subcutaneous fat type, visceral fat type, or abdominal muscle separation type depending on its shape. The subcutaneous fat-type abdominal obesity is a type with a large amount of subcutaneous fat in the abdomen, and is mainly found in women after childbirth or after menopause, and can be treated by ultrasound liposuction or systemically reducing body weight. The abdominal muscle separation type abdominal obesity is a type in which the stomach protrudes even if there is no fat, and can be easily treated by endoscopic surgery. The visceral fat-type abdominal obesity is a form of obesity that is very dangerous to health in the form of a lot of fat in the viscera of the abdomen, and liposuction or abdominal muscle strength exercises, which are general obesity treatment methods, do not help.

In a specific embodiment of the present invention, the present inventors obtained a hot water complex extract of Ogapi stem, arrowroot root, dulcis stem, Bokryeong, Deodeok root and jujube fruit, and ingested the hot water complex extract for 8 weeks for obese women As a result of measuring changes in body composition and physical strength, it was confirmed that not only body fat mass but also abdominal fat percentage significantly decreased after ingestion than before ingesting the hot water complex extract.

In addition, the present inventors measured the change in body composition after orally administering the hot water complex extract to experimental animals on a high fat diet for 8 weeks, compared to the high fat diet group, in the high fat diet group administered the hot water complex extract orally. It was confirmed that the weight gain decreased and the accumulation of visceral fat and subcutaneous fat was suppressed.

In addition, the present inventors measured the change in blood cholesterol after orally administering the hot water complex extract to experimental animals on a high fat diet for 8 weeks, compared to the high fat diet group, in the high fat diet group administered the hot water complex extract orally. It was confirmed that the total cholesterol content decreased.

Therefore, the present inventors have confirmed that the effect of significantly reducing body fat mass and abdominal fat percentage, the effect of inhibiting the accumulation of visceral fat and subcutaneous fat, and the effect of reducing blood cholesterol, the present invention The complex extract of can be usefully used as an active ingredient in a composition for preventing or treating metabolic diseases including obesity, especially abdominal obesity.

The pharmaceutical composition according to the present invention may further include suitable carriers, excipients, and diluents commonly used in the preparation of pharmaceutical compositions.

The pharmaceutical composition according to the present invention can be administered orally or parenterally, and when parenterally administered, the method of injecting skin external or intraperitoneal injection, rectal injection, subcutaneous injection, intravenous injection, intramuscular injection or intrathoracic injection is selected. It is preferable, but is not limited thereto.

The pharmaceutical composition according to the present invention is formulated in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories and sterile injectable solutions according to a conventional method. Can be used. Carriers, excipients and diluents that may be included in the composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oils. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and these solid preparations include at least one excipient, such as starch, calcium carbonate, and sucrose. ) Or lactose (lactose), gelatin, etc. are mixed to prepare. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, liquid solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweetening agents, fragrances, and preservatives may be included. . Preparations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like may be used.

The preferred dosage of the pharmaceutical composition according to the present invention varies depending on the condition and weight of the patient, the degree of the disease, the drug form, the route and duration of administration, but may be appropriately selected by those skilled in the art. However, for a desirable effect, the composition is preferably administered at 0.00001 to 1 g/kg per day, preferably 0.001 to 200 mg/kg, but is not limited thereto. The administration may be administered once a day, or may be divided several times. The above dosage does not in any way limit the scope of the present invention.

In addition, the present invention provides a health functional food for preventing or improving metabolic diseases, containing as an active ingredient a complex extract of Ogapi, Arrowroot, Hutgae Tree, Bokryeong, Deodeok and Jujube.

In the present invention, the complex extract is preferably extracted with water, C ₁ to C ₄ lower alcohol or a mixed solvent thereof, and more specifically, one selected from the group consisting of water, methanol, ethanol and a mixed solvent thereof It is preferably extracted with the above solvent, and more specifically, it is preferably extracted with water, but is not limited thereto.

The complex extract is 20 to 40 parts by weight of Ogapi, 15 to 35 parts by weight of arrowroot, 10 to 30 parts by weight of dulcis tree, 1 to 20 parts by weight of Bokryeong, 1 to 20 parts by weight of Deodeok, and 0.5 to It is preferable to be blended in 15 parts by weight, and 25 to 35 parts by weight of Ogapi, 20 to 30 parts by weight of arrowroot, 15 to 25 parts by weight of dulcis, 5 to 15 parts by weight of Bokryeong, 5 to 15 parts by weight of Deodeok, And it is more preferable that the jujube is blended in 1 to 10 parts by weight, and 28 to 32 parts by weight of Ogapi, 23 to 27 parts by weight of arrowroot, 18 to 22 parts by weight of birch, 8 to 12 parts by weight of Bokryeong, Deodeokyi It is more preferable that 8 to 12 parts by weight, and 3 to 7 parts by weight of jujube are blended, and 30 parts by weight of Ogapi, 25 parts by weight of arrowroot, 20 parts by weight of birch, 10 parts by weight of Bokryeong, 10 parts by weight of Deodeok It is most preferable to be blended in parts by weight, and 5 parts by weight of jujube, but is not limited thereto.

In addition, the Ogapi, Arrowroot, Hutgae Tree, Deodeok and Jujube may use one or more portions selected from the group consisting of leaves, stems, roots and fruits thereof, and more specifically Ogapi stem, Arrowroot, Hutgae tree trunk, Deodeok Roots and jujube fruits can be used.

In the present invention, the metabolic disease may be obesity, hyperlipidemia or hypercholesterolosis, but is not limited thereto.

In addition, the obesity may be specifically abdominal obesity, and the abdominal obesity may be subcutaneous fat type abdominal obesity, visceral fat type abdominal obesity or abdominal muscle separating type abdominal obesity, and more specifically, subcutaneous fat type abdominal obesity or visceral fat type. It could be abdominal obesity.

The complex extract of the present invention exhibits the effect of significantly reducing body fat mass and abdominal fat percentage, inhibiting the accumulation of visceral fat and subcutaneous fat, and reducing blood cholesterol, and therefore obesity, especially metabolic diseases including abdominal obesity It can be usefully used as an active ingredient in health functional foods for prevention or improvement.

The health functional food of the present invention may be added as it is to the complex extract or used with other foods or food ingredients, and may be appropriately used according to a conventional method.

There is no particular limitation on the type of food. Examples of such foods include drinks, meat, sausage, bread, biscuits, rice cakes, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, alcoholic beverages and vitamins. There is a combination drug, etc., and includes all health foods in the usual sense.

The mixing amount of the complex extract according to the present invention may be appropriately determined according to the purpose of use (for prevention or improvement). In general, the amount of the complex extract in the health food may be added in 0.01 to 15% by weight of the total food weight. However, in the case of long-term intake for the purpose of health and hygiene or for the purpose of health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

The health functional beverage composition of the present invention is not particularly limited to other ingredients other than those containing the above complex extract as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates, etc. have. Examples of the above-described natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, and the like; And polysaccharides, for example, common sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (taumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. .

In addition to the above, the food of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickeners (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, and the like may be contained. In addition, the complex extract of the present invention may contain flesh for the manufacture of natural fruit juice and fruit juice beverage and vegetable beverage. These components may be used independently or in combination. The proportion of these additives is not so important, but is generally selected from 0 to about 20 parts by weight per 100 parts by weight of the complex extract of the present invention.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Preparation Examples.

However, the following Examples, Experimental Examples, and Preparation Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples, Experimental Examples, and Preparation Examples.

< Example 1> Ogapi, arrowroot, Hazel tree , Bokryeong, Deodeok and the manufacture of complex extract containing jujube

Ultra-high-speed vacuum extractor (COSMOS-660, Kyungseo machine, Incheon, Korea) 30% by weight (1.65 kg) of Ogapi stems, 25% by weight of arrowroot roots (1.375 kg), 20% by weight (1.1 kg) of dulcis stems, Bokryeong 10 60 L of purified water was added to wt% (0.55 kg), 10 wt% (0.55 kg) of deodeok roots, and 5 wt% (0.275 kg) of jujube fruits, followed by extraction at 103° C. for 5 hours. After extraction, it was packaged using an automatic spout pouch packaging machine (SPS-A430, Sungil system corp, Daegu, Korea) and used for the experiment.

< Comparative example 1> Preparation of Ogapi extract

Into an ultra-high-speed vacuum low-temperature extractor (COSMOS-660, Kyungseo machine, Incheon, Korea), 5.5 kg of Ogapi stems and 60 L of purified water were added and extracted in the same manner as described in <Example 1> to prepare Ogapi extract.

< Experimental example 1> Confirmation of the effect of improving obesity of complex extract

In order to confirm the effect of improving obesity of the complex extract prepared in <Example 1>, the body composition changes before and after ingestion of the complex extract prepared in <Example 1> were measured for women in their 40s to 50s. I did.

Specifically, the test was conducted on 21 women in their 40s to 50s with good health. Before performing the test, using InBody720 (InBody Co., Ltd., Cheonan, Korea), the subjects' body weight, body water, lean mass, body fat mass, abdominal fat percentage, BMI (body mass index: body weight (kg), height (m)) Body composition factors including the value divided by the square of (kg/m ² )) were measured, and the measurement time was measured in the morning (10:00 ~ 12:00) before meals in consideration of the subject's physical condition. Among the above body composition factors, BMI is the most widely used obesity index due to its high correlation with body fat percentage among physique indexes. In addition, in Lee and Cho, if the BMI is less than 25 and the body fat percentage is less than 20%, it is "normal", and if the BMI is 25 or higher and the body fat percentage is less than 20%, it looks fat, but the body fat is in the normal range of "appearance obesity" and BMI is 25 If the body fat percentage is less than 20%, it is classified as "obesity". Therefore, for more accurate analysis, body moisture, lean mass, body fat mass, body fat percentage, and abdominal fat percentage were checked. Here, it is known that the amount of body water and body fat are in an inverse proportion, and the water content is largely dependent on the fat content. In addition, fat-free mass refers to a value excluding fat mass from body weight.

By measuring the BMI and body fat percentage before performing the test, it was confirmed that among 21 subjects, 11 were in the body fat-type obese group and 10 were in the obese group.

Then, the subjects who completed the measurement were allowed to consume 100 mL of the complex extract prepared in Example 1 each for 8 weeks in the morning and evening, and then the body composition factors were measured in the same manner as described above. It was compared with the change in body composition before ingestion. On the other hand, all subjects participating in the test were given the same dietary regimen as usual during the test, but drug use and injection were prohibited.

In addition, the mean and standard deviation of each body composition factor was obtained using the SPSS v12.0 (SPSS Inc., Chicago, IL, USA) statistical program, and the comparison before and after consumption was t-test with p<0.05, Significance was verified at the level of p<0.01 and p<0.001.

As a result, as shown in Table 1, when the complex extract of the present invention was ingested for 8 weeks, there was no significant difference in body weight change, but body fat mass significantly decreased from 20.17±6.57 kg to 19.40±6.48 kg, and The body fat percentage was also significantly decreased from 32.45±6.29% to 31.46±6.40%. In particular, it was confirmed that the percentage of abdominal fat was significantly reduced from 0.88±0.06 before ingestion to 0.86±0.05 even though subjects did not exercise regularly while ingesting the complex extract of the present invention. Therefore, through the above results, it was confirmed that the complex extract of the present invention has the effect of improving obesity, especially abdominal obesity.

Before intake 8 weeks after consumption Change t-value p-value Age (years) 49.81±4.06 Height(cm) 156.38±4.86 156.53±5.05 - -1.245 0.228 Weight (kg) 60.72±9.58 60.18±9.50 - 1.537 0.140 BMI(kg/m2) 24.77±3.44 24.51±3.42 - 1.613 0.122 Body moisture (L) 29.77±3.12 29.92±3.14 0.15±0.64 -1.096 0.286 Fat free (kg) 40.56±4.29 40.78±4.32 0.22±0.85 -1.183 0.251 Body fat mass (kg) 20.17±6.57 19.40±6.48 -0.77±1.29 2.724 ^* 0.013 Body fat percentage (%) 32.45±6.29 31.46±6.40 -0.99±1.30 3.495 ^** 0.002 Abdominal fat percentage 0.88±0.06 0.86±0.05 -0.06±0.20 3.894 ^** 0.001

^* p<0.05, ^** p<0.01

< Experimental example 2> Confirmation of the effect of improving visceral fat type or subcutaneous fat type abdominal obesity of the complex extract

<2-1> Production of experimental animals

Twenty four five-week-old C57BL/6J mice (Daehan Biolink, Chungbuk) were purchased and stabilized while maintaining a 12-hour interval at 24±2° C. for one week. Then, each of 6 animals was randomly divided into the experimental groups of the following [Table 2]. Distilled water, the complex extract prepared in <Example 1>, or the Ogapi extract prepared in <Comparative Example 1> was orally administered to each experimental group once a day for 8 weeks. In addition, the normal group was supplied with a normal diet, and the remaining experimental groups were supplied with a high fat diet containing 45% fat (Research D12451) for 8 weeks.

Experimental group Sample administered Diet Normal 1 distilled water for 8 weeks Normal diet High fat diet. 1 distilled water for 8 weeks 45% high fat diet High fat diet + Oga skin extract administration group Oral administration of Ogapi extract 18 mg/kg/day for 8 weeks 45% high fat diet High fat diet + complex extract administration group Orally administered complex extract 18 mg/kg/day for 8 weeks 45% high fat diet

<2-2> Confirmation of weight loss due to complex extract

For each of the experimental groups of Experimental Example <2-1>, the body weight was measured before oral administration of the sample and after oral administration for 8 weeks.

As a result, as shown in Table 3, it was confirmed that the weight gain of the high fat diet group was the highest at 5.36±0.31 g. On the other hand, it was confirmed that the weight gain in the high fat diet + complex extract group showed lower weight gain than the high fat diet group. In addition, the dietary intake of the high-fat diet group and the high-fat diet + complex extract administration group appeared similar, and it was confirmed that the dietary intake did not affect the anti-obesity effect of the complex extract.

Experimental group Initial weight
(g) Final weight
(g) Weight gain
(g) Dietary intake
(g/day/mice) Normal 16.48±0.71 19.93±0.44 3.51±0.34 2.51±0.07 High fat diet. 16.53±0.68 22.04±1.29 5.36±0.31 2.27±0.03 High fat diet + Oga skin extract administration group 16.52±0.61 22.65±1.60 5.06±0.92 2.31±0.27 High fat diet + complex extract administration group 16.50±0.57 20.34±0.57 3.94±0.10 2.29±0.17

<2-3> Confirmation of visceral fat reduction by complex extract

After oral administration of the sample to each of the experimental groups of Experimental Example <2-1> for 8 weeks, changes in visceral fat were confirmed.

Specifically, samples were orally administered to each of the experimental groups of Experimental Example <2-1> for 8 weeks, and then fasted for 12 hours before sacrifice. On the last day of the experiment, ether was inhaled and heart blood was collected under anesthesia. In order to examine the weight of adipose tissue, visceral fat was extracted, washed with physiological saline, and water was removed with a filter paper, and the weight was measured.

As a result, as shown in Table 4, it was confirmed that the visceral fat of the high fat diet group was significantly increased compared to the normal group. On the other hand, it was confirmed that the visceral fat of the high fat diet + complex extract administration group was 0.0906±0.0213 g, which was significantly reduced compared to the high fat diet group and the high fat diet + Oga skin extract administration group.

Experimental group Visceral fat (g) Normal 0.0478±0.0103 High fat diet. 0.2425±0.0701 High fat diet + Oga skin extract administration group 0.1562±0.0480 High fat diet + complex extract administration group 0.0906±0.0213

<2-4> Confirmation of the effect of reducing the size of fat cells by the complex extract

After oral administration of the sample to each of the experimental groups of Experimental Example <2-1> for 8 weeks, the size change of adipocytes was confirmed.

Specifically, after orally administering a sample to each of the experimental groups of Experimental Example <2-1> for 8 weeks, the visceral adipose tissue of mice in each experimental group was carefully removed and washed with PBS. For histological analysis, it was fixed in 10% formalin solution and embedded in paraffin. A section was cut to a thickness of 4 um, and the cells were stained with hematoxylin-eosin (HE). After that, adipocytes were observed with a slide scanner (Aperio AT2, Leica bio systems Ltd., Germany).

As a result, as shown in FIG. 1, it was confirmed that the size of the fat cells in the group administered with the high-fat diet + complex extract was smaller than that of the group administered with the high-fat diet and the high-fat diet + organic skin extract.

Through the above results, it was confirmed that the complex extract of the present invention can more effectively improve visceral fat-type or subcutaneous fat-type abdominal obesity by inhibiting the accumulation of visceral fat and subcutaneous fat.

< Experimental example 3> Hyperlipidemia and Hypercholesterolosis Checking the improvement effect

After oral administration of the samples to each of the experimental groups of Experimental Example <2-1> for 8 weeks, changes in triglyceride and cholesterol contents were confirmed.

Specifically, after orally administering a sample to each of the experimental groups of Experimental Example <2-1> for 8 weeks, on the last day of the experiment, all animals were cardiac blood sampled under ether inhalation anesthesia, and plasma was separated from the collected blood by centrifugation, The content of high density lipoprotein cholesterol (HDL), total cholesterol, and triglyceride in plasma was measured using a pre-prepared Asan kit (Asan Pharmaceutical, Korea).

As a result, as shown in Table 5, it was confirmed that the total cholesterol content was highest in the high fat diet group, while the content was significantly decreased in the high fat diet + complex extract administration group. In addition, it was confirmed that the HDL content of the high fat diet + complex extract administration group was similar to that of the high fat diet group, while the HDL content decreased in the high fat diet + Oga skin extract administration group.

Experimental group Total cholesterol
(mg/dl) Triglycerides
(mg/dl) HDL
(mg/dl) Normal 91.21±6.04 109.27±3.97 48.73±5.39 High fat diet. 122.19±15.77 139.47±8.01 55.12±5.61 High fat diet + Oga skin extract administration group 78.29±11.16 79.84±6.40 42.68±4.73 High fat diet + complex extract administration group 65.86±4.11 90.78±17.85 51.31±6.96

Through the above results, it was confirmed that the complex extract of the present invention can improve hyperlipidemia and hypercholesterolosis due to the content of blood cholesterol by reducing the total cholesterol content without affecting the content of HDL.

Hereinafter, examples of preparation for the composition of the present invention are illustrated.

< Manufacturing example 1> The complex extract of the present invention Preparation of pharmaceutical preparations containing as active ingredients

<1-1> Powdery Produce

Complex extract of the present invention 10 mg

1 g lactose

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

<1-2> Preparation of tablets

0.1 mg of the complex extract of the present invention

100 mg corn starch

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, tablets were prepared by tableting according to a conventional tablet preparation method.

<1-3> Preparation of capsules

0.1 mg of the complex extract of the present invention

100 mg corn starch

100 mg lactose

2 mg of magnesium stearate

After mixing the above ingredients, the gelatin capsules were filled according to a conventional capsule preparation method to prepare a capsule formulation.

<1-4> Preparation of ring

Complex extract of the present invention 1 mg

1.5 g lactose

1 g glycerin

0.5 g xylitol

After mixing the above components, it was prepared so as to be 4 g per pill according to a conventional method.

<1-5> Preparation of granules

0.15 mg of the complex extract of the present invention

50 mg soybean extract

200 mg glucose

600 mg starch

After mixing the above ingredients, 100 mg of 30% ethanol was added, dried at 60°C to form granules, and then filled into a cloth.

< Manufacturing example 2> Preparation of health food containing the complex extract of the present invention as an active ingredient

<2-1> Preparation of flour food

0.5 to 5.0 parts by weight of the complex extract of the present invention was added to flour, and bread, cake, cookies, crackers and noodles were prepared using this mixture.

<2-2> soup And the production of gravies

0.1 to 5.0 parts by weight of the complex extract of the present invention was added to soups and meat juices to prepare health-promoting meat products, noodle soups, and juices.

<2-3> Ground Of ground beef Produce

Ground beef for health promotion was prepared by adding 10 parts by weight of the complex extract of the present invention to ground beef.

<2-4> Manufacture of dairy products

5 to 10 parts by weight of the complex extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<2-5> Linear Produce

Brown rice, barley, glutinous rice, and adlay were gelatinized by a known method, dried, and then roasted, and then prepared into a powder having a particle size of 60 mesh with a grinder.

Black soybeans, black sesame seeds, and perilla seeds were also steamed and dried by a known method, and then roasted, and then prepared into powder having a particle size of 60 mesh by a grinder.

The composite extract of the present invention was concentrated under reduced pressure in a vacuum concentrator, sprayed and dried with a hot air dryer, and the resulting dried product was pulverized with a grinder to a particle size of 60 mesh to obtain a dry powder.

It was prepared by mixing the grains, seeds and the complex extract of the present invention prepared above in the following ratio.

Grains (30 parts by weight of brown rice, 15 parts by weight of barley, 20 parts by weight of barley)

Seeds (perilla 7 parts by weight, black soybean 8 parts by weight, black sesame 7 parts by weight),

Complex extract of the present invention (3 parts by weight),

Ganoderma lucidum (0.5 parts by weight),

Rehmannia (0.5 parts by weight)

< Manufacturing example 3> Containing the complex extract of the present invention as an active ingredient Health drink Produce

<3-1> Health drink Produce

Instant sterilization is performed by homogeneously mixing subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and 100 ml of the complex extract of the present invention. Then, it was prepared by packaging it in a small container such as a glass bottle or a plastic bottle.

<3-2> Preparation of vegetable juice

Vegetable juice was prepared by adding 100 ml of the complex extract of the present invention to 1,000 ml of tomato or carrot juice.

<3-3> Preparation of fruit juice

Fruit juice was prepared by adding 100 ml of the complex extract of the present invention to 1,000 ml of apple or grape juice.

Claims (9)

Containing 20 to 40 parts by weight of Ogapi, 15 to 35 parts by weight of arrowroot, 10 to 30 parts by weight of dulcis tree, 1 to 20 parts by weight of Bokryeong, 1 to 20 parts by weight of Deodeok, and 0.5 to 15 parts by weight of jujube as an active ingredient , Obesity, hyperlipidemia, and a pharmaceutical composition for preventing or treating metabolic diseases selected from the group consisting of hypercholesterolosis.
The pharmaceutical composition for preventing or treating metabolic diseases according to claim 1, wherein the complex extract is extracted with water, C ₁ to C ₄ lower alcohol or a mixed solvent thereof.
delete The pharmaceutical composition for preventing or treating metabolic diseases according to claim 1, wherein the Ogapi, Arrowroot, Hutgae Tree, Deodeok and Jujube are extracted from a site selected from the group consisting of leaves, stems, roots and fruits.
delete The pharmaceutical composition for preventing or treating metabolic diseases according to claim 1, wherein the obesity is abdominal obesity.
The pharmaceutical composition for preventing or treating metabolic diseases according to claim 6, wherein the abdominal obesity is selected from the group consisting of subcutaneous fat type abdominal obesity, visceral fat type abdominal obesity, and abdominal muscle separation type abdominal obesity.
Containing 20 to 40 parts by weight of Ogapi, 15 to 35 parts by weight of arrowroot, 10 to 30 parts by weight of dulcis tree, 1 to 20 parts by weight of Bokryeong, 1 to 20 parts by weight of Deodeok, and 0.5 to 15 parts by weight of jujube as an active ingredient , Obesity, hyperlipidemia, and a health functional food for preventing or improving metabolic diseases selected from the group consisting of hypercholesterolosis.
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