KR20160139953A - Anti-obesity composition comprising Cirsium japonicum flower extract as effective component - Google Patents

Abstract

The present invention relates to a composition for preventing or treating obesity which contains a hot water extract of Cirsium japonicum flower as an active ingredient. The composition containing an extract of Cirsium japonicum flower as an active ingredient of the present invention reduces weight and visceral fat as well as blood triglycerides and LDL cholesterol level such that the composition is effective in inhibiting obesity, and thus, the composition can be effectively used in the pharmaceutical industry and food industry for obesity inhibition.

Description

The present invention relates to an anti-obesity composition comprising Cirsium japonicum flower extract as an active ingredient,

The present invention relates to a method for the treatment of cirsium The present invention relates to a composition for preventing, ameliorating or treating obesity, which contains, as an active ingredient, a hydrothermal extract of Japonica japonicum .

In recent years, the modernization of the economy has led to a rise in the level of living standards, which leads to an increase in the intake of fat and sugar and a decrease in the consumption of fiber. In particular, It is a reality that the obesity population is rapidly increasing due to the lifestyle of modern people who hardly exist. Obesity, especially abdominal obesity, is emerging as a serious social problem as the intake of high calorie foods increases and the intake of fiber decreases. Obesity refers to the state of overgrowth of fat tissue, and the increase in body weight due to obesity is mostly due to the increase in fat.

The hazard of obesity is that obesity itself causes constipation, indigestion and gastrointestinal disturbance due to the pressure of abdomen caused by adipose tissue, which is a risk factor for many adult diseases. Obesity is known to be the source of all diseases including cancer, arteriosclerosis, heart disease, hypertension, diabetes, and other diseases and complications. In 1996, the World Health Organization (WHO) warned that obesity is a disease that requires treatment. A study of 750,000 people found that men and women over 40% of their average body weight had a 1.9-fold increase in mortality rates compared to those of normal weight.

Obesity is an accumulation of excess fat in the body, the cause of fat accumulation in the body is to eat excess carbohydrates (carbohydrates) or to overdose. The mechanism that reaches obesity is that the sugar contained in the food is digested and becomes monosaccharide, absorbed into the body through the small intestine, and the blood sugar is elevated and insulin secreted by the stimulus acts on the adipocyte so that monosaccharide Fat cells will be accepted and converted to fat. In addition, triglyceride, which is the highest calorie of foods, is decomposed by lipase of the pancreas and absorbed through the small intestine, resulting in increased storage calories due to excessive intake of calories. That is, obesity is caused by excessive fat intake.

Currently, there are appetite suppressants, body heat stimulants, diuretics, and so on. Most of the obesity drugs are used as appetite suppressants in the US market for 100 million dollars a year. However, since appetite suppressants for obesity treatments exhibit side effects such as elevated blood pressure, diarrhea, constipation, insomnia, and anxiety, there is a growing interest in using various food materials that have been proven for safety and physiological function for many years to suppress obesity. Currently, dietary fiber, herbal medicines, and various food products have been developed for obese people. However, when eating these foods, it is difficult to live a normal life due to the lack of nutrients due to lack of nutrients. It is known to quickly become obese again.

Cirsium I have japonicum . ussuriense ) is a perennial herb that belongs to the Compositae family, and has been used as a medicinal herb in the herbaceous area or under the ground (root). Flowers, stems, and seeds are collected from May to June in the flowering season, and the roots are collected in autumn, dried and extracted with hot water or alcohol to be used for treatment of hematemesis, hematuria, hepatitis, and hypertension I have been.

Korean Patent No. 1266998 discloses a composition for prevention and treatment of weight loss and obesity, which contains an extract as an active ingredient, and Korean Patent No. 1177379 discloses a composition for preventing and treating weight loss and obesity, An anti-obesity composition containing an extract and a bean sprout extract 'is disclosed, but no anti-obesity composition containing the thistle flower extract of the present invention as an effective ingredient has been disclosed.

The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a method for the treatment of obesity, which comprises the step of reducing the amount of triglyceride and LDL cholesterol in blood, Completed.

In order to solve the above problems, the present invention thistle (Cirsium The present invention provides a health functional food composition for preventing or ameliorating obesity, which contains, as an active ingredient, a hydrothermal extract of japonicum flower.

In addition, the present invention provides a pharmaceutical composition for preventing or treating obesity containing Cirsium japonicum flower hydrothermal extract as an active ingredient.

Thistle of the present invention (Cirsium japonicum ) as an active ingredient is useful as a pharmaceutical and food industry for the prevention of obesity because it reduces weight and visceral fat as well as reduces triglyceride and LDL cholesterol content in blood have.

FIG. 1 is a graph comparing weight gain of experimental animals according to administration of thistle root, leaf or flower extract. FIG. normal control; Standard diet group, HF control; High-bay mode, apigenin; Positive control.
2 is a graph comparing visceral fat contents of experimental animals according to administration of thistle root, leaf or flower extract. normal control; Standard diet group, HF control; High-bay mode, apigenin; Positive control.
3 is a graph comparing blood triglyceride (TG) contents of experimental animals with thistle root, leaf or flower extract administration. normal control; Standard diet group, HF control; High-bay mode, apigenin; Positive control.
FIG. 4 is a graph comparing HDL (high density lipoprotein) cholesterol contents in blood of experimental animals according to the administration of thistle root, leaf or flower extract. normal control; Standard diet group, HF control; High-bay mode, apigenin; Positive control.
FIG. 5 is a graph comparing the LDL (low density lipoprotein) cholesterol content in blood of an experimental animal according to administration of thistle root, leaf or flower extract. normal control; Standard diet group, HF control; High-bay mode, apigenin; Positive control.

According to an aspect of the invention, the invention thistle (Cirsium The present invention provides a health functional food composition for preventing or ameliorating obesity, which contains, as an active ingredient, a hydrothermal extract of japonicum flower.

In the composition of the present invention, the thistle flower hot-water extract may be an extract prepared by adding water to dried thistle flower and then hot-extraction at 80 to 120 ° C for 0.5 to 4 hours, more preferably dried Which is prepared by adding water to thistle flowers and then hot-extraction at 100 ° C for 1 hour, but the present invention is not limited thereto.

The thistle flower hydrothermal extract may reduce the body weight and visceral fat, reduce the content of blood triglyceride and LDL cholesterol, and increase the content of HDL cholesterol, but the present invention is not limited thereto.

Preferably, the thistle flower hydrothermal extract is contained in an amount of 0.1 to 100% by weight based on the weight of the composition, but the present invention is not limited thereto, and the composition may be any one selected from a beverage, a ring, a tablet, a capsule, But the present invention is not limited thereto. The health functional food composition of the present invention may be added as it is or may be prepared together with other food or food ingredients, and may be suitably prepared according to a conventional method. Examples of foods to which the health functional food composition of the present invention can be added include dairy products including caramel, meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, , Beverage, tea, drink, alcoholic beverage, and vitamin complex, and includes all the health foods in a conventional sense. That is, there is no particular limitation on the kind of the food.

The health functional food is not particularly limited as long as it can be ingested to prevent or improve obesity.

The food may contain various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alkynic acid and its salts, Stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices and vegetable drinks. The above components can be used independently or in combination. In addition, the health functional food composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient. The natural carbohydrates may be selected from the group consisting of glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, Polysaccharides such as cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.

The invention also thistle (Cirsium The present invention provides a pharmaceutical composition for preventing or treating obesity, which contains, as an active ingredient, a hydrothermal extract of japonicum flower.

Preferably, the thistle flower hydrothermal extract may be an extract prepared by adding water to dried thistle flowers and then subjecting to hot water extraction at 80 to 120 ° C for 0.5 to 4 hours. More preferably, water is added to the dried thistle flower And then extracted with hot water at 100 ° C for 1 hour. However, the present invention is not limited thereto.

The thistle flower hydrothermal extract may reduce the body weight and visceral fat, reduce the content of blood triglyceride and LDL cholesterol, and increase the content of HDL cholesterol, but the present invention is not limited thereto.

The pharmaceutical composition may be used to prevent, ameliorate, or treat various metabolic diseases such as diabetes, hyperlipidemia, heart disease, stroke, arteriosclerosis, fatty liver, etc. derived from obesity-related diseases such as obesity and obesity.

The thistle flower hydrothermal extract is preferably contained in an amount of 0.1 to 100% by weight based on the weight of the composition, but is not limited thereto.

The compositions of the present invention may comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions. The pharmaceutically acceptable carrier to be contained in the pharmaceutical composition of the present invention is one which is usually used at the time of formulation and includes saline solution, sterilized water, Ringer's solution, buffered saline solution, dextrose solution, maltodextrin solution, glycerol, ethanol, But are not limited to, sucrose, sucrose, sucrose, sorbitol, mannitol, starch, acacia, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, syrup, methylcellulose, methylhydroxybenzoate, Hydroxybenzoate, talc, magnesium stearate and mineral oil, and the like.

The pharmaceutical composition of the present invention may further contain an antioxidant, a buffer, a bacteriostatic agent, a diluent, a surfactant, a binder, a lubricant, a wetting agent, a sweetener, a flavoring agent, an emulsifier, a suspending agent or a preservative.

The pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably administered orally. A suitable dose of the pharmaceutical composition of the present invention may be variously prescribed depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, .

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Manufacturing example  1: Thistle flower Heat number  Preparation of extract

Thistle used in the experiment (Cirsium I have japonicum . ussuriense ) was supplied from the Imsil Medicinal Herb Farming Association in Ohsu-myeon, Imsil County, Jeollabuk-do. Dried thistle roots, leaves and flowers were quantified as 400g, 400ml of distilled water was injected and boiled for 1 hour. The obtained extract was filtered using 0.45μm filter, and then lyophilized and recovered in powder form. The recovered extracts were stored at -20 ° C and used in experiments.

Experimental Method

A) Experimental animals

Four weeks old C56BL / 6 mice were purchased from Central Laboratories Inc. (Korea) and maintained for 1 week while adjusting the intensity of light in a 12 hour cycle maintaining the appropriate temperature (22 ± 2 ° C) and humidity (50 ± 5% . C56BL / 6 mice were divided into 6 control groups, normal control group, HF control group, 45% fat calories group, and high-fat diet group and thistle root, The high - fat diet and apigenin - treated group were fed for 12 weeks. The thistle extracts were administered by oral gavage daily at a dose of 10 ml / kg with 200 mg / kg body weight of roots, leaves and flowers, respectively, and apigenin was administered at a dose of 10 mg / kg body weight. The standard diet group and high fat diet group were orally administered the same amount of distilled water.

B) Weight measurement

Body weights were measured on the first day of the experiment and then once a week for 12 weeks until the end of the experiment.

C) Blood sampling and blood lipid concentration analysis

Blood was collected at 12 weeks after the end of the experiment for 12 hours and then anesthetized with ether. Euthanized blood was collected from the ophthalmic vein, euthanized, and heart, liver, pancreas, spleen, Of formalin. The blood was centrifuged at 2,500 rpm for 15 minutes to separate the serum and stored at -70 ° C until biochemical analysis of the serum.

(Total cholesterol), high-density lipoprotein (HDL) cholesterol, and high-density lipoprotein (HDL) cholesterol were measured by a kit (AM 202-K, AM 203KTC, AM 157S-K, Asan Pham, LDL (low density lipoprotein) cholesterol and TG (triglyceride) were measured. LDL content was calculated as {TC-HDL- (TG / 5)} by the formula of Friedewald et al.

D) Statistical processing

All data were expressed as means ± SD (mean ± SD). Statistical comparisons were made using student's t-test.

Example 1: Changes in body shape and visceral fat induced by administration of thistle extract

The body and visceral fat changes after 12 weeks of administration of thistle root, leaf and flower extracts are shown in FIGS. 1 and 2. As shown in Fig. 1, the thistle flower extract-treated group showed a significant change pattern and showed more effective weight loss than the positive control group, apigenin-treated group. As shown in FIG. 2, the visceral fat change after 12 weeks was significantly different from the thistle root and leaf extracts in the thistle flower extract-treated group, Respectively. From the above results, it can be seen that thistle flower extract is more effective for anti-obesity than root or leaf extract.

Example 2: Changes in blood lipid composition upon administration of thistle extract

As shown in FIG. 3, the content of triglyceride (TG) in the thistle root, leaf and flower extract groups decreased significantly after 12 weeks of administration of thistle extract. Especially, the triglyceride content of the thistle flower extract group was the lowest And was similar to the positive control group apigenin. In addition, the changes of HDL and LDL contents after 12 weeks of administration of thistle extract were significantly different in the thistle leaves or flower extract administration groups as shown in FIGS. 4 and 5. As shown in Fig. 4, all of the thistle root, leaf or flower extract administration groups significantly increased the HDL content. In the case of thistle root extract, there was no significant change in LDL content, whereas in the case of thistle leaf or flower extract-treated group, the effect of reducing LDL content was remarkable (FIG. 5). In particular, it was confirmed that the effect of reducing the LDL content in the group treated with thistle flower extract was most effective.

Claims (5)

Cirsium japonicum ) as an active ingredient for the prevention or amelioration of obesity. [Claim 2] The method according to claim 1, wherein the thistle flower hydrothermal extract is prepared by adding water to dried thistle flowers and then hot-extraction at 80 to 120 DEG C for 0.5 to 4 hours. Composition. The method according to claim 1, wherein the thistle flower hydrothermal extract lowers body weight and visceral fat, decreases blood triglyceride and LDL cholesterol content, and increases HDL cholesterol content. Functional food composition. Cirsium The present invention relates to a pharmaceutical composition for preventing or treating obesity, which contains, as an active ingredient, a hydrothermal extract of japonicum flower. The pharmaceutical composition for preventing or treating obesity according to claim 4, further comprising a carrier, an excipient or a diluent in addition to the active ingredient.

Images