KR20150063183A - Cosmetic or food composition comprising natural plants fermented extracts for reducing abdominal obesity - Google Patents

Abstract

The present invention relates to a natural plant fermented extract exhibiting an abdominal slimming effect and, more specifically, to a composition for reinforcing an abdominal slimming effect, wherein licorice and dried orange peel fermented extracts are included to control the decomposition and differentiation of fat cells.

Description

[0001] The present invention relates to a cosmetic or food composition for reducing abdominal obesity containing a natural plant fermentation extract,

The present invention relates to a natural plant fermentation extract showing abdominal slimming efficacy, and more particularly, it relates to a plant fermentation extract which exhibits an abdominal slimming effect, and more particularly, ≪ / RTI >

Abdominal obesity is easily manifested when there is a decrease in basal metabolism due to erroneous dietary life, uneventful life, excessive stress, and lack of exercise. Clinically, the degree of obesity is measured by the ratio of waist to hip ratio. In males, the W / H ratio is classified as abdominal obesity when the ratio is 0.9 or more, and when it is 0.85 or more, it is classified as abdominal obesity . In males, visceral fat is 2 ~ 3 times more than females basically, and abdominal obesity is likely to occur because they are often consumed by drinking and smoking and high fat foods. In women, postmenopausal follicular atresia results in decreased estrogen synthesis and secretion, and increased gonadal hormone secretion from the pituitary gland, leading to increased abdominal fat tissue (Choi Y , Yoon et al. Korean J. Nutr. 34 (3): 322-329, 2001 and Turcato E. et al. J Intern Med 241 (5): 363-372, 1997).

It is important to prevent the accumulation of fat in the body by reducing food intake and increasing energy consumption, although obesity is being actively and diverse in many countries. Particularly, it is known that exercise is the most effective way to increase energy consumption. However, due to the development of traffic communication, the amount of exercise is gradually decreasing and it is difficult to exercise regularly every day in modern life. Also, improvement of obesity through dietary control is not easy to reduce the amount of food, and it is not easy due to frequent eating out and overfilling of high-calorie instant food. Other therapies include pharmacotherapy and surgery, but both are difficult to implement in terms of safety and economics. Therefore, interest in slimming products and slimming foods is continuously increasing due to safety and relative ease of carrying out.

Korean Laid-Open Patent Publication No. 2013-0099572 (Publication date: 2013.09.06)

The inventors of the present invention discovered that the mixture of fermented extract of natural plant, fermented licorice and dermis, and the mixture of fermented licorice fermented extract and fermented dermis extract were superior in fat lipid degradation and adipocyte differentiation inhibition in fat cells, and completed the present invention .

Accordingly, an object of the present invention is to provide a natural plant fermentation extract which alleviates abdominal obesity by inhibiting lipolysis in adipocytes and differentiation of adipocytes.

In order to achieve the above object, the present invention provides a cosmetic composition or a food composition containing a mixture of licorice fermented extract and dermis fermented extract.

The use of the natural plant fermentation extract of the present invention can inhibit lipolysis in adipocytes and differentiation of adipocytes. Accordingly, the natural plant fermentation extract can be used as an effective ingredient of a cosmetic composition or a food composition for abdominal slimming

The present invention relates to a natural plant fermentation extract showing abdominal slimming efficacy and a cosmetic composition or food composition containing the same.

Hereinafter, the present invention will be described more specifically.

The " licorice ( Glycyrrhiza uralensis Fischer ) is a perennial plant of soybean with roots and rootstocks of licorice; the root of the rootstock is the licorice root; the end of root or the root of root is licorice root; , Licorice, which is a part of a material that appears like a dark brown resin contained in roots or rootstocks, and is included in this application.

The term "dermal" in this invention means the jingyul (Citrus proclamation Hort . ex Tanaka ) is a tangerine tree belonging to the mulberry tree, which is distributed in Jeju Island. The fragrance and flavor of the fruit is unique and belonged to the commodity among the local specialties that were offered as tax in the past. It can not be concluded when the citrus began to grow on Jeju Island, but it is said in the literature on citrus as 1052 (Goryeongjong 6) It is presumed that it has been developed and therefore the citrus has been cultivated before that, so the cultivation history of Jeju citrus can be regarded as very old. Traditionally, the old peels of citrus (Chen Tachibana - Jeju Citrus Nutrition Materials, Sanchung - Chosun Dynasty Annals, Products - Jeju dialect, Sanchung - China, Sunki - Japanese pronunciation, Citrus sunki Hort. Ex Tanaka) come. The mandarin bark of mandarin duck is described in the mandarin bark of mandarin duck in the Joseon Dynasty. The "mandarin bark" is recorded as "the bark of mandarin orange."

The term " licorice fermentation " or " dermis fermentation " of the present invention means that the licorice or dermis is replaced with an alpha-amylase, a beta -amylase, a gluco-amylase, protease, cellulase, pectinase, alpha-glucosidase, beta-galactosidase, 1,4-beta-D-glucanase (1,4-β-D-glucanase) or a mixed enzyme thereof to obtain a fermented product.

The "licorice fermentation extract" or "dermis fermentation extract" may be prepared by dissolving a percolated fermented extract of licorice or mandarin orange, which is a target to be extracted, with an alcohol having 1 to 4 carbon atoms such as methanol and ethanol, 70% ethanol (alcohol), acetone, ethyl acetate, Chloride, water, or a mixed solvent thereof, and fractions further purified with the above-listed solvents in the extracts.

In the present invention, the licorice fermentation extract or the dermis fermentation extract extracted from the fermentation extract of licorice or dermis produced by the above method may be contained in an amount of 0.000001 to 30.0 wt%, respectively, based on the total weight of the composition, Or dermis fermentation extract in an amount of 0.01 to 5.0% by weight based on the total weight of the composition. If the licorice fermentation extract or the dermis fermentation extract is contained in an amount of less than 0.000001 wt%, the effect of the ingredient is insufficient, and if it is contained in an amount of more than 30.0 wt%, problems may arise.

In the present invention, the licorice fermentation extract and the dermis fermentation extract are mixed in a weight ratio of 0.01 to 100: 1. In order to maximize the synergistic effect of the combination of the two components, the licorice fermentation extract and the dermis fermentation extract may be mixed in a weight ratio of 0.1 to 10: 1.

The composition of the present invention may be formulated as an external preparation for skin, in particular as a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any form suitable for topical application, for example, as a solution, an emulsion obtained by dispersing an oil phase in water phase, an emulsion obtained by dispersing water phase in water phase, a suspension, a solid, a gel, Pastes, foams, or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, to the extent that the main effect is not impaired. The composition according to the present invention may further comprise a humectant, an emollient, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by a person skilled in the art within the range not impairing the object and effect of the present invention, and the amount thereof may be from 0.01 to 5% by weight, specifically from 0.01 to 3% by weight based on the total weight of the composition .

In addition, the composition of the present invention may be prepared by using the composition of the present invention optionally in combination with other excipients, and may be appropriately formulated into a dry powder, granules, capsules, tablets, pills, beverages, emulsion liquids, diet bars, Or confectionary, and can be used as health functional foods and general foods.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Preparation Example 1] Preparation of licorice fermented extract

1 kg of dried licorice was pulverized with a blender, 5 L of water was added, stirred and extracted, and then immersed at 15 캜 for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through filter cloth, PEXTINEX 0.1% was added to the separated filtrate, and the mixture was stirred at 40 ° C for 3 days. After centrifugation, the supernatant was removed and the precipitate was extracted with 99% ethanol. This was centrifuged again, and only the supernatant was separated and concentrated under reduced pressure to obtain 125 g of licorice fermented extract.

[Preparation Example 2] Preparation of dermal fermentation extract

500 g of completely dried Tangerine peel was crushed to 10 mesh and 950 g of distilled water was added and 15 g of? -Amylase was treated. The mixture was fermented in a shaking incubator (LSB.045S, KOR) for 20 hours in a stirred incubator at a temperature of 70 ° C. The fermentation broth was frozen at -80 ° C for 24 hours in a cryogenic freezer (ilshinbiobase 905, KOR) and lyophilized for 48 hours using a freeze dryer (ilshinbiobase PVTFD20R, KOR) to produce a fermented powder. The fermented powder was extracted with 20% by volume of 70% ethanol while stirring at room temperature for 20 hours. Filtration was performed using Hyundai Micro NO. 100 (3um) filter paper, the solvent in the filtrate was distilled off using a vacuum concentrator, and lyophilized to obtain 63 g of a dermis fermentation extract.

[Experimental Example 1] Evaluation of lipid differentiation inhibitory effect in 3T3-L1 adipocytes

The mouse preadipocyte 3T3-L1 (CL-173) used in the experiment was purchased from ATCC (American Type Culture Collection, Manassas, Va., USA) and the cells were inoculated into 10% fetal bovine calf serum It was inoculated on adding a DMEM (Dulbecco's modified Eagle's medium ) culture medium, and cultured at 37 ℃, the conditions of 10% CO _2. 3T3-L1 progenitor adipocytes were plated on 12 or 24 well plates and plated at 100% confluency with MDI (0.5 mM 3-isobutyl-1-methylxanthine (IBMX), 1 μM dexamethasone, 1 μg / mL insulin) for 2 days, and then cultured for 2 days with 10% FBS DMEM containing 1 μg / mL insulin. The cells were then replaced with 10% FBS DMEM and cultured for 2 days. During the induction of adipocyte differentiation, the experimental material, Example 1 and Comparative Examples 1 to 4, were treated with each culture. DMSO was used as a negative control, and 1 μg / mL and 3 μg / mL of retinoic acid, which are known standard substances for inhibiting lipid differentiation, were used as a positive control. At the completion of differentiation, the degree of adipocyte differentiation was observed through Oil-Red O staining. That is, after differentiation, the medium was removed, washed with PBS, and the cells were fixed with 10% formaldehyde solution for 30 minutes at room temperature. Then, the solution was removed, washed twice with PBS, washed twice with 70% alcohol, and stained with Oil Red O solution. After staining the cells with a microscope and dissolving them in isopropyl alcohol, absorbance was measured at 490 nm.

Sample information Low performance (%) Negative control group DMSO 12.4 Positive control 1 Retinoic acid 1 μg / ml 25.8 Positive control 2 Retinoic acid 3 μg / ml 33.0 Comparative Example 1 Licorice fermented extract 10 μg / mL 17.2 Comparative Example 2 Dermis fermented extract 10 μg / mL 29.4 Comparative Example 3 Licorice fermented extract 20 μg / mL 25.8 Comparative Example 4 Dermal fermentation extract 20 μg / mL 31.1 Example 1 Licorice fermentation extract + dermis fermentation extract 10 μg / mL 46.6

As shown in Table 1, it was confirmed that the licorice fermented extract of Comparative Example 1 and the dandruff fermentation extract of Comparative Example 2 inhibited adipocyte differentiation. In particular, it was found that when the licorice fermented extract of Example 1 and the dandruff fermentation extract were mixed at a ratio of 1: 1, they had an excellent effect as compared with Comparative Examples 1 and 2. In addition, in Example 1, the liposome fermentation extract and the dermis fermentation extract showed more excellent inhibitory effect on the differentiation of the adipocyte compared to the case of the 2-fold concentration of Comparative Example 3, which is synergistic effect when mixed with the licorice fermentation extract and the dermis fermentation extract I could confirm.

[Experimental Example 2] Evaluation of lipolysis effect in 3T3-L1 adipocytes

3T3-L1 adipocytes completely differentiated in 12- or 24-well plates were treated with DMEM containing 2% BSA (fatty acid-free bovine serum albumin) in Example 1 and Comparative Examples 1 to 4 for 4 hours Lt; / RTI > The amount of glycerol liberated by neutral lipid degradation was determined by reacting the cell supernatant (culture medium) with free glycerol reagent (Sigma, USA) for 15 minutes. DMSO was used as a negative control, and isoproterenol and caffeine 10 μg / mL were used as a positive control. For the determination of glycerol, 25 μg / 10 μL of a standard glycerol (standard glycerol; Sigma) was reacted in the same manner as above to measure the absorbance at 540 nm. The content of glycerol was calculated by the following formula.

Sample information Glycerol O.D Control group DMSO 0.092 Comparative Example 1 Licorice fermented extract 10 μg / mL 0.119 Comparative Example 2 Dermis fermented extract 10 μg / mL 0.130 Comparative Example 3 Licorice fermented extract 20 μg / mL 0.178 Comparative Example 4 Dermal fermentation extract 20 μg / mL 0.195 Example 1 Licorice fermentation extract + dermis fermentation extract 10 μg / mL 0.249

As shown in Table 2, the fermented licorice extract of Comparative Example 1 and the fermented dandelion extract of Comparative Example 2 showed lipolytic effect. In particular, it was found that when the licorice fermentation extract of Example 1 and the dandruff fermentation extract were mixed and treated at a ratio of 1: 1, they had an excellent effect as compared with Comparative Examples 1 and 2. In addition, Example 1 exhibited a better lipolytic effect than the case of the licorice fermentation extract and dermis fermentation extract of Comparative Example 3, which was twice the concentration of licorice, showing synergistic effects when the licorice fermentation extract and the dermis fermentation extract were mixed there was.

From the above results, it was confirmed that the composition containing the extract extracted from the fermented product of licorice and dermis according to the present invention is a useful raw material for abdominal slimming efficacy because it has remarkably excellent fat decomposition effect and lipid differentiation inhibiting effect in fat cells.

Claims (6)

A composition for external application for skin for reducing abdominal obesity comprising an extract of fermented licorice and a fermented extract of dermis as an active ingredient. [Claim 3] The composition for external application for skin of abdominal obesity according to claim 1, wherein the licorice fermentation extract and the dermis fermentation extract are contained in an amount of 0.000001 to 30.0 wt% based on the total weight of the composition. The composition for external application for skin of abdominal obesity according to claim 1, wherein the licorice fermentation extract and the dermis fermentation extract are mixed in a weight ratio of 0.01 to 100: 1. A fermented extract of licorice and a fermented extract of dermis as an active ingredient. The food composition according to claim 4, wherein the licorice fermentation extract and the dermis fermentation extract are contained in an amount of 0.000001 to 30.0% by weight based on the total weight of the composition, respectively. The food composition according to claim 4, wherein the licorice fermentation extract and the dermis fermentation extract are mixed in a weight ratio of 0.01 to 100: 1.