KR102149254B1 - Pharnaceutical composition for prevention or treatment of diabetes comprising the mixed extract of vegetable natural products having the effect on removal of swelling and health functional food for prevention or improvement of diabetes comprising the same - Google Patents

Abstract

The present invention relates to a pharmaceutical composition and health functional food for preventing or treating/improving diabetes (diabetes) containing as an active ingredient a mixed extract of a vegetable natural product having excellent swelling removal, immunity strengthening, and metabolism enhancing activity, and more detailed For example, beta-amylase (β-) containing as an active ingredient a hot water extract of a mixture of vegetable natural products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger, or an ethanol extract further obtained therefrom. amylase) and alpha-glucosidase (α-glucosidase) through the inhibitory activity of the prevention or treatment / improvement of diabetes pharmaceutical composition and health functional food. Mixed extracts of vegetable natural products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger as active ingredients of the antidiabetic composition of the present invention, as demonstrated through the examples of the present specification, total poly While the phenol and total flavonoid content is very high, the total sugar and reducing sugar content is very low, and it exhibits antidiabetic activity through beta-amylase and alpha-glucosidase inhibitory activity. In addition, the active ingredient of the present invention does not exhibit any hemolytic activity against human red blood cells, has excellent thermal stability, and does not show loss of beta-amylase and alpha-glucosidase inhibitory activity even in acidic conditions of pH 2 and plasma, It is a very useful invention in the pharmaceutical industry and the food industry because it can be prepared in a form that can be taken at any time such as extract, powder, pills, and tablets.

Description

Pharmaceutical compositions and health functional foods for preventing or treating diabetes containing mixed extracts of plant-based natural products that are effective in removing swelling. SWELLING AND HEALTH FUNCTIONAL FOOD FOR PREVENTION OR IMPROVEMENT OF DIABETES COMPRISING THE SAME}

The present invention relates to a pharmaceutical composition and health functional food for preventing or treating/improving diabetes (diabetes) containing as an active ingredient a mixed extract of a vegetable natural product having excellent swelling removal, immunity strengthening, and metabolism enhancing activity, and more detailed For example, beta-amylase (β-) containing as an active ingredient a hot water extract of a mixture of vegetable natural products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger, or an ethanol extract further obtained therefrom. amylase) and alpha-glucosidase (α-glucosidase) through the inhibitory activity of the prevention or treatment / improvement of diabetes pharmaceutical composition and health functional food.

Diabetes, one of the most common diseases in modern people, is a type of metabolic disease caused by insufficient secretion of insulin or the inability of insulin to perform its normal function, and is characterized by high blood glucose concentration. Causes symptoms and signs.

Diabetes is divided into type 1 and type 2. Type 1 diabetes is caused by the inability to produce insulin at all due to genetic effects, and type 2 diabetes is caused by poor insulin function and insulin resistance. ) Is known as the cause. In particular, type 2 diabetes is known to be largely affected by environmental factors such as high calorie, high fat, high protein diet, lack of exercise, and stress due to westernization of diet.

For the treatment of diabetes, insulin injection is essential in the case of type 1 diabetes, and lifestyle correction and drug treatment are necessary in the case of type 2 diabetes. Representative insulin secretion stimulators (e.g., repaglinide, mitigl) Nide) and a delaying agent for carbohydrate absorption in the small intestine (glucobi: ingredient name-acarbose, bason: ingredient name-voglibose), and the like are used.

Meanwhile, a variety of chronic diseases are increasing due to the advancement of society and instant food culture, and a lot of research is focused on disease prevention and improvement using natural medicinal crops whose safety has been confirmed rather than pharmaceuticals.

For example, Buttercup ( Oenanthe javanica (Blume) DC.) is a dicotyledonous plant, a perennial plant of the Aperaceae family. In oriental medicine, it is called carpuncle and swimming. In Donguibogam, it quenchs thirst, clears the head, removes alcohol poisoning, jaundice, and women's diseases. , It is recorded that it is effective for headache and vomiting after drinking. In addition, in recent studies, the effects of improving liver function, detoxification, and releasing heavy metals are known.

Burdock ( Arctium lappa ) is a dicotyledonous plant, a biennial plant of the Asteraceae family, and its origin is Europe, and is widely distributed in Siberia, Manchuria, and Korea. In Korea, it is cultivated mainly in Gyeongnam, and it is a root vegetable that is boiled or eaten by roots because of its characteristic aroma and chewy taste. It is usually not eaten raw, but is salted, boiled in vinegar, or boiled in soy sauce for consumption. In oriental medicine, the root is called woobanggeun, the fruit is called woobangja, and the stem is called Aksilkyung, and its uses are strictly classified. The burdock basement, which is mainly used for food, has a viscous and bitter taste, so it is mainly boiled in soy sauce and edible.Inulin, vitamin B1, acetic acid, arctigenin, beta-carotene, butyric acid, coffee acid, chlorogenic acid, caustic acid, isovare It is known to contain a large amount of minerals such as ic acid, lauric acid, lignin, myristic acid, propionic acid, sitosterol, stigmasterol and copper, chromium, manganese, phosphorus, selenium, silicon, and zinc. Therefore, burdock is known to be excellent in antioxidant, toxin removal, cancer cell growth inhibition, blood sugar control and weight loss.

Pumpkin ( Cucurbita moschata ) is a vine-like annual plant of the gourd family, native to tropical and South America, but is widely cultivated in Korea. Mature pumpkin contains a large amount of vitamins A and vitamins B and C, so it is very important as a source of vitamins. It contains beta carotene to help prevent aging, and it contains a large amount of dietary fiber to help improve intestinal health and constipation.

The leaf of Plantago asiatica L. is a perennial plant of the Plantago asiatica family. It is called plantain because of its tough leaves, and leaves and seed barks (Psyllium husk) are used for edible and medicinal purposes. In oriental medicine, it is used for diuresis, diarrhea, cystitis, urinary tract, etc., and is known to have a function of activating liver function.

Red beans ( Phaseolus radiatus L.) is an annual plant of the rosewood leguminous family and is cultivated mainly in Northeast Asia such as Korea, China, and Japan, and is the second most demanded bean in Korea after beans. Red beans are similar in shape and structure to beans, but have a red luster and, unlike beans, have a white band in the center, and contain 8-9% moisture, 68% carbohydrates, and 20% protein. Very good nutritionally. In particular, red beans contain a large amount of various vitamins including vitamin B1, minerals such as potassium, calcium, and phosphorus, and lysine, which is insufficient in rice, so it is effective in recovering from beriberi disease and fatigue, and helping protein production by resolving amino acid shortages. It has a beneficial function of promoting carbohydrate metabolism and is used as a food material for nutritional supplementation. Red beans have a heat-coagulant protein outside the cell wall uniquely among beans, and since they are preferentially thermally denatured when heated, they do not become like grass and have a peculiar structure in which sediments can be made.Mainly used for making red bean porridge, red bean sediment, yokan, and frozen dessert. It has been used as, and in recent years, it has been developed as a variety of processed foods such as red bean beverages and fermented paste foods using red beans. In oriental medicine, red beans are called Jeoksodu and are used to treat diarrhea, dysentery, swelling, and obesity. Boiled red beans have been used habitually as a diet drink and for removing swelling through diuresis and facilitation in the private sector.

Black beans ( Glycine max (L.) Merr) are called black beans, seoritae, black beans, etc., and have a large amount of protein, vitamins and aging inhibitors such as isoflavones, and hair loss prevention ingredients such as cysteine. In addition, black beans have been used for vision enhancement, detoxification, and treatment of sogal (消渴), and they are positioned as important functional materials in traditional Korean food culture as they are called'Yak beans'. As a result of recent research, it is known that it is effective in preventing hair loss and removing edema.

Jujube ( Zizyphus jujuba ) is the fruit of the jujube tree of the buckthorn family and is called wood wheat, millet, and hongjo. It is an essential fruit for domestic ceremonial occasions. The flesh mainly contains sugar, and also contains mucilage, neunggeumsan, and juseoksan. Seeds contain betulin, beturic acid, and fat. Jujube is not only edible raw, but also dried and used for confectionery, cooking, and medicinal purposes. In addition, it is processed and used as jujube liquor, jujube tea, jujube vinegar, jujube porridge, etc., especially in oriental medicine, it is used to treat and relieve diuresis, tonic and soothing and reliever, pneumonia, bronchitis, and cough.

Ginger ( Zingiber officinale ) is one of the subtropical and tropical perennial herbaceous plants of the Zingiberaceae family, and the basement rhizome has a peculiar taste and aroma, and is used as a three major spice along with garlic and red pepper in Korea. Ginger is also used as a food, and it is also used as a seasoning in various food industries such as kimchi, salted fish, and confectionery. In addition, it is processed into products such as dry ginger (healthy or dry powder), oleoresin (ginger-containing paper), and essential ginger oil, and is used as a food material and cosmetic material (Seonhee Kim 2013, Ph.D. thesis at Hanyang University). In fact, ginger is known to have various physiological activities that are described as preventing white sand (hundred diseases) in the herbaceous gangmok, and in oriental medicine, it is known that it has excellent effects on dry placebo, indigestion, vomiting, and pain. The herbal name of ginger is [Health], and the general public often calls it [Saeang]. In the Goryeo Dynasty, it was novel enough to be used as a royal tribute, and it is introduced as a'remedy from God' in the ancient medical book of India [Ayurdeva].

Studies related to the antidiabetic activity of a single plant-based natural product include "Effects of Onion, Apple, Water Parsley, and Cabbage on Blood Sugar Control in Diabetic Patients" (Song Mi-kyung, 2012, Master's Thesis at Gyeonggi University), "Burdock Root Ethanol Extract Effect of this Streptozotocin-Induced Diabetic Rat on Antioxidation" (Ok-Kyung Kim, 2016, Journal of the Korean Oil Chemists, 33, 435-440), "Effect of Dietary Pumpkin Powder on Growth and Lipid Oxidation in Diabetic Rats" (Kyung-soon Lee et al., 2003. The Korean Nutrition Society Conference 308), "Effects of Lowering Blood Glucose and Insulin Sensitivity of Rat Eye Bean Powder Using Streptozotocin-Induced Diabetes Model" (Daehoon Lee et al., Journal of the Korean Society of Food Science and Nutrition, 2004, 33, 1618-1625), " The Effect of Fermented Extracts of Jujube Pulp and Leaves on Hyperlipidemia and Diabetes" (Yoonhee Choi, 2009, Ph.D. thesis at Wonkwang University), and "The effect of shogaol on various organs of diabetic mice" (Hyeyoon Choi, 2017. Master's thesis at Kyungpook National University) ), and the like, and related patents include Korean Patent No. 10-0194375, "Method for manufacturing health supplement foods containing herbal medicines using pumpkin extract as an active ingredient", and Patent No. 10-1190624 "Anthocyanin extracted from the skin of black beans. A pharmaceutical composition for preventing and treating diabetes containing" and the like are known.

In addition, as an extract targeting a complex composition of various natural substances, Korean Patent Registration No. 10-1536974 "A composition for preventing and improving diabetes containing a mixed extract of Yeoju, Cornus, Schisandra chinensis", Registered Patent No. 10-0956278 No. "Composition for the treatment or prevention of diabetes or diabetic complications using a complex herbal extract as an active ingredient", consisting of red beans, sticky black rice and sticky barley rice in Registration Patent No. 10-1001705, or with black tae, halmac, brown rice and glutinous rice Consisting "composition for the prevention and improvement of obesity, hyperlipidemia and diabetes" is disclosed, and in Korean Patent Publication No. 10-2002-0094179, Cheoncho, Sukjihwang, Ginger, Cornus, Sanyak, Mokdanpi, Maekmundong, Cheonhwabun, Taeksa, Baekbokryeong, Hwangnyeon , "Health Supplements for Diabetes" containing powders such as black beans, and Korean Patent Publication No. 10-2006-0025914, Cordyceps, Tosaja, Maekmundong, Jimo, Sukjihwang, Seontoe, Gucheok, Jahwanggi, Red Soybean, Sanyak, Dansam and Sesuoro A composition for preventing and treating diabetes comprising "a complex herbal extract (divided number)" is known.

However, until now, there are no patents or studies on the organoleptic and antidiabetic activity of extracts prepared using natural vegetable products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, soybeans, and ginger at the same weight ratio. .

KR 10-1536974 B KR 10-0956278 B KR 10-2006-0025914 A KR 10-2002-0094179 A

Lee, Gyeong-soon et al., 2003, Effect of Pumpkin Powder-Added Diet on Growth and Lipid Oxidation in Diabetic Rats, Korean Nutrition Society Conference, 308 Mikyung Song, 2012, Effect of Onion, Apple, Parsley, Cabbage on Blood Glucose Control in Diabetic Patients, Master Thesis, Kyunggi University

The present invention was devised to solve the problems of the prior art as described above, and the problem to be solved in the present invention is a mixed extract of vegetable natural substances including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, soybeans and ginger It is intended to provide a pharmaceutical composition and health functional food for the prevention or treatment and improvement of diabetes, including.

In order to solve the above problems, the present invention is a pharmaceutical for the prevention or treatment of diabetes containing as an active ingredient a mixed extract of vegetable natural products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger The composition is provided.

The mixed extract as an active ingredient of the pharmaceutical composition of the present invention is preferably obtained by extraction with hot water.

The mixed extract as an active ingredient of the pharmaceutical composition of the present invention is preferably obtained by extracting with hot water and then with ethanol.

In addition, the present invention provides a health functional food for preventing or improving diabetes, containing a mixed extract of vegetable natural products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger as an active ingredient.

The mixed extract as an active ingredient of the health functional food of the present invention is preferably obtained by extracting with hot water.

The mixed extract as an active ingredient of the health functional food of the present invention is preferably obtained by extracting with hot water and then with ethanol.

Mixed extracts of vegetable natural products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger as active ingredients of the antidiabetic composition of the present invention, as demonstrated through the examples of the present specification, total poly While the phenol and total flavonoid content is very high, the total sugar and reducing sugar content is very low, and it shows antidiabetic activity through beta-amylase and alpha-glucosidase inhibitory activity. In addition, the active ingredient of the present invention does not exhibit any hemolytic activity against human red blood cells, has excellent thermal stability, and does not show loss of beta-amylase and alpha-glucosidase inhibitory activity even in acidic conditions of pH 2 and plasma, It is a very useful invention in the pharmaceutical industry and the food industry because it can be prepared in a form that can be taken at any time such as extract, powder, pills, and tablets.

1 shows the supernatant of the present invention (a) extract powder and (b) 10% aqueous solution,
2A and 2B show the degree of inhibition of β-amylase and α-glucosidase of the extract of the present invention, 1: active control (acarbose, 0.5mg/ml), 2: hot water extract of the present invention (0.5mg/ml) , 3: ethanol extract of the present invention (0.5 mg/ml), 4: commercial product 1 (0.5 mg/ml) described in Example 1 of the present invention, 5: commercial product 2 (0.5 mg/ml) described in Example 1 of the present invention mg/ml) respectively.

Hereinafter, the present invention will be described in detail.

In order to test the antidiabetic activity of a mixture of parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger, the inventors of the present invention include parsley, burdock, pumpkin, plantain leaves, red beans, black beans, dates and ginger. Each was added in the same weight ratio, watered, extracted at high temperature for 16 hours or more to prepare an extract, and spray-dried to prepare a powdery extract, and the organoleptic and antidiabetic activity were evaluated to obtain the extract. It was confirmed that the antidiabetic activity was excellent. The extract was to be used as a pharmaceutical composition and health functional food for preventing or treating diabetes and improving diabetes by confirming that the extract does not exhibit any hemolytic activity on human red blood cells, but has excellent thermal stability and acid stability. .

Specifically, the present inventors use parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger, which are known to have various physiological activities in the private sector, to develop an antidiabetic composition, the 7 kinds of edible or medicinal vegetable Each natural product was mixed at the same weight ratio, added a 15-fold weight ratio thereto, and extracted at a high temperature of 100° C. for 16 hours or longer to prepare an extract, which was spray-dried to prepare the extract of the present invention. It was confirmed that the extract exhibited excellent organoleptic and antidiabetic activity, and did not show any hemolytic activity against human red blood cells even at a concentration of 1mg/ml, and thus did not induce acute toxicity.

Accordingly, the present invention provides a pharmaceutical composition for the prevention or treatment of diabetes containing a mixed extract of vegetable natural products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger as an active ingredient.

The mixed extract as an active ingredient of the pharmaceutical composition of the present invention is preferably obtained by extraction with hot water.

The mixed extract as an active ingredient of the pharmaceutical composition of the present invention is preferably obtained by extracting with hot water and then with ethanol.

In addition, the present invention provides a health functional food for preventing or improving diabetes, containing a mixed extract of vegetable natural products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger as an active ingredient.

The mixed extract as an active ingredient of the health functional food of the present invention is preferably obtained by extracting with hot water.

The mixed extract as an active ingredient of the health functional food of the present invention is preferably obtained by extracting with hot water and then with ethanol.

Hereinafter, a method for preparing a mixed extract of natural vegetable products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger according to the present invention will be described in more detail.

The inventors of the present invention, in order to achieve the object of the present invention, the step of slicing selected, washed parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger: 100° C. Extracting by heat treatment in a sealed condition for at least 16 hours; Then preparing an extract from the hot water extract; Experiments in the steps of evaluating the functional and antidiabetic activity of the composition and the stability of the active substance were performed.

The seven kinds of natural products may be mixed at the same weight, and the hot water extract may be prepared as an extract in powder form through a conventional powdering process such as vacuum drying, freeze drying, or spray drying. In addition, the powdery extract prepared from the hot water extract may be additionally extracted with ethanol to obtain an ethanol extract.

In addition, the extract of the present invention is not decomposed by various degrading enzymes in plasma, and maintains activity even at a heat treatment of 100° C. and a pH in the stomach of the human body at pH 2.

The extract of the present invention exhibits potent β-amylase and α-glucosidase inhibitory activity against starch degrading enzymes, and thus prevents and treats diabetic complications such as type 1 diabetes, type 2 diabetes or diabetic retinopathy, diabetic nephropathy, etc. It can be used as a material for pharmaceutical compositions and health functional foods related to improvement.

Pharmaceutical compositions containing the active ingredients of the present invention are oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., according to a conventional method for each purpose of use, and injections of sterile injectable solutions It may be formulated and used in various forms such as, and may be administered orally or administered through various routes including intravenous, intraperitoneal, subcutaneous, rectal, and topical administration.

Such pharmaceutical compositions may further include a carrier, excipient, or diluent, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, Starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, amorphous cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil And the like. In addition, the pharmaceutical composition of the present invention may further include a filler, an anti-aggregating agent, a lubricant, a wetting agent, a fragrance, an emulsifying agent, a preservative, and the like.

In a preferred embodiment, solid formulations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid formulations include at least one excipient, such as starch, calcium carbonate, and the like, in the pharmaceutical composition. It is formulated by mixing sucrose, lactose, gelatin, and the like. Further, in addition to simple excipients, lubricants such as magnesium stearate and talc may be used.

As a preferred embodiment, as a liquid formulation for oral use, a suspension, a liquid formulation, an emulsion, a syrup, and the like may be exemplified, and various excipients other than water and liquid paraffin, which are commonly used simple diluents, such as wetting agents, sweetening agents, Fragrances, preservatives, etc. may be included.

As a preferred embodiment, the preparation for parenteral administration may include a sterile aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a lyophilized agent, a suppository, and the like. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyloleate. Injectables may contain conventional additives such as solubilizing agents, isotonic agents, suspending agents, emulsifying agents, stabilizing agents, and preservatives.

The active ingredient of the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" refers to an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is the type of disease, severity, drug activity, Sensitivity to drugs, time of administration, route of administration and rate of excretion, duration of treatment, factors including drugs used concurrently, and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with a conventional therapeutic agent, and may be administered single or multiple. It is important to administer an amount capable of obtaining the maximum effect in a minimum amount without side effects in consideration of all the above factors, and this can be easily determined by a person skilled in the art.

As a preferred embodiment, the effective amount of the active ingredient in the pharmaceutical composition of the present invention may vary depending on the age, sex, and body weight of the patient, and generally 1 to 5,000 mg, preferably 100 to 3,000 mg per kilogram of body weight daily Alternatively, it may be administered every other day or divided into 1 to 3 times a day. However, since it may increase or decrease depending on the route of administration, the severity of the disease, sex, weight, age, etc., the dosage amount is not limited by any method.

The pharmaceutical composition of the present invention can be administered to a subject through various routes. All modes of administration can be expected and may be administered by, for example, oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

In the present invention, "administration" means providing a predetermined substance to a patient by any suitable method, and the route of administration of the pharmaceutical composition of the present invention is oral or parenteral through all general routes as long as it can reach the target tissue. It can be administered orally. In addition, the composition of the present invention may be administered using any device capable of delivering an active ingredient to target cells.

In the present invention, the "object" is not particularly limited, but includes, for example, human, monkey, cow, horse, sheep, pig, chicken, turkey, quail, cat, dog, mouse, mouse, rabbit, or guinea pig And, preferably, a mammal, more preferably a human.

In addition, the health functional food of the present invention can be variously used for foods and beverages effective in preventing or improving thrombosis. Foods containing the active ingredient of the present invention include, for example, various foods, beverages, gum, tea, vitamin complexes, health supplements, and the like, and may be used in the form of powders, granules, tablets, capsules or beverages. .

In general, the active ingredient of the present invention may be added in an amount of 0.01 to 50% by weight of the total food weight, and the health beverage composition may be added in an amount of 0.02 to 50g, preferably 0.3 to 10g, based on 100ml.

The health functional food of the present invention may contain the compound as an essential component in the indicated ratio as an additional component, as well as food supplementary additives acceptable for food, such as natural carbohydrates and various flavoring agents.

Examples of the natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and conventional sugars such as polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol.

Taumatin as the flavoring agent; Natural flavoring agents such as stevia such as rebaudioside A or glysirhizin, and synthetic flavoring agents such as saccharin and aspartame may be used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention includes various nutrients, vitamins, minerals, synthetic flavoring agents and natural flavoring agents, and other flavoring agents, coloring agents and thickeners, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloids. It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health functional food of the present invention may contain pulp for the production of natural fruit juices, fruit juice drinks, and vegetable drinks. These components may be used independently or in combination. The proportion of these additives is generally selected from 0.01 to about 20 parts by weight per 100 parts by weight of the active ingredient of the present invention.

Hereinafter, the present invention will be described in more detail through examples. The following examples are only one preferred specific example of the present invention, and the scope of the present invention is not limited to the scope of the following examples.

[Example]

Example 1: Preparation and sensory evaluation of extract

In order to develop an antioxidant composition using natural edible or medicinal mixed extracts with secured safety, parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujubes and ginger were purchased, respectively, and then foreign substances were removed and selected. After slicing and adding the 7 kinds of natural products at the same weight ratio, respectively, 15 times the weight ratio thereof was added, and hot water extraction was performed at 100°C for 16 hours or more under sealed conditions. At this time, until extraction with hot water for less than 14 hours, the extraction was not performed properly because the decomposition of fibrin such as plantain leaves did not appear, and a characteristic green smell appeared, resulting in low sensory properties. Therefore, when extracted for more than 16 hours, the extract is highly enhanced with sufficient hot water extraction and sensory improvement. On the other hand, the mixed extract is a raw material extract of a product known under the brand name'Raon Tea'.

In order to compare the characteristics of the extracts of the present invention, two kinds of existing commercially available products manufactured from mixed extracts of medicinal or edible natural substances (commercial product 1:'Mokae' of Jeongjin Agricultural Corporation, containing Jocheong, pear, bellflower, and Schisandra chinensis. , Commercial product 2:'Gongmi' product of Mega Bio Forest Co., Ltd., containing dermis, bamboo leaves, Astragalus, Bokryeong, Jeonju, wheat, Gyeji, Yeonjayuk, Nogeun, Rehmannia, Jinju and corn beard) and extracts of the present invention Physicochemical properties and color difference analysis were performed, and the results are shown in Table 1. In addition, the extract powder and the 10% aqueous solution supernatant of the present invention are shown in FIG. At this time, color difference analysis was measured using a Hunter Color Difference meter (Super color SP-80 Colormeter, Tokyo Denshoku Co., Japan), and brightness (white 100 to 0 black), redness (red 100 to -80 green), Yellowness (yellow 70 ~ -80 black) was measured. At this time, the chromaticity of the standard white plate was set as 92.44 for L value, -0.06 for a value, and 1.35 for b value, and the average value was calculated by measuring three times per sample, and the color difference (△E) was calculated using the following equation. And calculated.

[Table 1] Physicochemical properties and color difference analysis of extract

As shown in Table 1, in the case of the extract of the present invention, it exhibited a very high brix of 95% or more, and the pH was 4.9 similar to that of the existing commercial product 2. However, it can be seen that the acidity is relatively low, 0.8, so it can be easily consumed. Table 2 shows the results of functional evaluation after adjusting the existing product and the extract of the present invention to 9.5 brix. At this time, the sensory evaluation was evaluated on a 7-point scale by dividing into taste, aroma, and overall preference targeting 10 experts in food and nutrition majors and 40 consumers in their twenties.

[Table 2] Sensory evaluation of extract

As shown in Table 2, the extract of the present invention was found to have an overall preference increase of 1.36 points or more compared to the conventional natural compound extract, commercial product 1, and increased sensory properties in all of taste, aroma, throat and mouth residual. . In addition, the extract of the present invention has low irritation and low sugar content due to low acidity, and it is determined that continuous drinking is easy.

Example 2: Analysis of the components of the extract

The content of total polyphenols, total flavonoids, total sugars and reducing sugars was measured by component analysis of the extract of the present invention prepared in Example 1. For the total polyphenol content, 50 μl of Folin-ciocalteau and 100 μl of Na ₂ CO ₃ saturated solution were added to 400 μl of the extraction sample solution, and allowed to stand at room temperature for 1 hour, and the absorbance was measured at 725 nm. Tannic acid was used as a standard reagent. For the total flavonoid content, each sample was extracted with methanol for 18 hours, 4 ml of 90% diethylene glycol was added to 400 μl of the filtered extraction sample solution, 40 μl of 1N NaOH was added, and the absorbance was measured at 420 nm after 1 hour reaction at 37°C. Rutin was used as a standard reagent. Reducing sugar was quantified by DNS method and total sugar was quantified by phenol-sulfuric acid method. As a sample control, two kinds of commercially available concentrated stick products of similar natural compound extract were used, and the extract of the present invention was used by preparing a powder of 10% solution.

[Table 3] Results of component analysis of extract

As a result, as shown in Table 3, the total polyphenol content of the extract of the present invention was 2.10mg/g, the total flavonoid content was 0.915mg/g, and the total sugar and reducing sugar contents were 47.1mg/g and 8.2, respectively. It appeared in mg/g. In the case of the polyphenol content, compared to the existing commercial product 2, the level was 68%, but compared to the commercial product 1, the amount was 1.78 times higher. However, the total flavonoid content was 4.9-35.2 times higher than that of the existing product, and the total sugar and reducing sugar content contained 9-20% and 2.5-7.6%, respectively. Therefore, compared with existing commercial products, the extract of the present invention was characterized by having a high flavonoid content and a low sugar content.

Example 3: Antidiabetic evaluation of extract

The antidiabetic activity of the extract of the present invention prepared in Example 1 was evaluated as β-amylase inhibitory activity and α-glucosidase inhibitory activity, and the results are shown in Table 4 and FIGS. 2A and 2B.

First, β-amylase (4-alpha-D-glucan maltohydrolase) inhibitory activity was obtained by mixing 2.5 μL of sample and 25 μL of β-amylase (0.25 U/mL) diluted with 50 mM phosphate buffer (pH 6.8) at 37°C for 10 minutes. After the first reaction, 25 μL of 0.5% soluble starch (Samchun Chemicals Co., Korea) was added and the second reaction was performed at 37°C for 10 minutes, and then the reaction was stopped by heating at 100°C for 5 minutes, and 150 μL of DNS in the reaction solution (3,5-dinitrosalicylic acid, Sigma Co., st. Louis, USA) solution was added, heated at 100° C. for 5 minutes to develop color, and then allowed to cool at room temperature. The color developing solution was calculated by measuring the absorbance at 540 nm.

On the other hand, α-glucosidase inhibitory activity was evaluated using pNPG (p-nitrophenol glucoside; Sigma Co., USA), and α-glucosidase (0.25 U/ml) diluted with 2.5 μl of sample and 50 mM sodium acetate buffer (pH 5.6). ) 25 μl of the mixture was first reacted at 37° C. for 10 minutes, and 25 μl of a 1 mM pNPG solution was added, followed by a second reaction at 60° C. for 10 minutes. Thereafter, 25 μl of 1M NaOH was added to stop the reaction, and the inhibition rate was calculated by measuring the absorbance at 405 nm.

[Table 4] Antidiabetic activity of extract

As a result, as shown in Table 4, acarbose, an antidiabetic drug used in clinical practice, inhibited β-amylase and α-glucosidase by 55.5% and 69.7%, respectively, at a concentration of 0.5 mg/ml, confirming the basis for clinical use. The extract of the present invention also inhibited β-amylase and α-glucosidase by 12.7% and 8.9% at a concentration of 0.5mg/ml, respectively, showing very strong starch degradation inhibitory activity. In particular, when the powder of the hot water extract of the present invention was extracted again with ethanol, β-amylase and α-glucosidase were inhibited by 25.8% and 15.8%, respectively, at a concentration of 0.5mg/ml, showing very strong antidiabetic activity. On the other hand, commercially available natural product complex extract products did not show any antidiabetic activity at the same concentration.

Example 4: Evaluation of human red blood cell hemolytic activity of extract

Parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger, which are the raw materials of the extract of the present invention, have been used for edible and medicinal purposes since the past, and the extract is judged to have no specific toxicity. In order to evaluate the potential acute toxicity of the extract of the present invention, the hemolytic activity of human red blood cells was evaluated, and the results are shown in Table 5. At this time, the hemolytic activity was evaluated according to the previous report (In-Chang Jung, Ho-Yong Son, 2014, Korean J. Microbiol. Biotechnol. 42: 285~292), and simply 100 μl of human red blood cells washed three times with PBS was measured in 96-well. After adding 100 μl of sample solution of various concentrations to the microplate, the reaction was carried out at 37°C for 30 minutes.After that, the reaction solution was centrifuged for 10 minutes (1,500 rpm), and 100 μl of the supernatant was transferred to a new microtiter plate, and the degree of hemoglobin leakage due to hemolysis was measured. It was measured at 414 nm. DMSO (2%) was used as a solvent control for the sample, and Triton X-100 (1 mg/ml) was used as an experimental control for hemolysis of red blood cells. Hemolytic activity was calculated using the following formula.

[Table 5] Hemolytic activity of human red blood cells of the extract

First, it was confirmed that DMSO and distilled water used as control cells had no red blood cell hemolytic activity, and Triton X-100 hemolytic 100% red blood cells at a concentration of 1 mg/ml. In the case of the empoteracin ratio, which is used as an antifungal and anticancer agent, a strong hemolytic activity of 85.3% was observed even at a concentration of 0.025mg/ml. On the other hand, in the case of the commercial product 1 and the commercial product 2, which are the extract of the present invention and the commercial natural product complex extract, neither hemolytic activity appeared. Therefore, it is determined that the extract of the present invention will not exhibit a separate problem of causing acute toxicity.

Example 5: Plasma, acid and thermal stability evaluation of extract

Plasma stability, thermal stability, and acid stability for antioxidant activity were confirmed for the extract of the present invention prepared in Example 1 above. The extract exhibited high stability because there was no significant decrease in scavenging ability for DPPH anions and ABTS cations even after 1 hour heat treatment at 100 °C, 1 hour treatment at pH 2 (0.01M HCl), and 1 hour treatment in plasma. . Therefore, the extract of the present invention is expected to maintain excellent antioxidant activity during digestion and absorption process and food manufacturing process.

Claims (4)

A pharmaceutical composition for preventing or treating diabetes containing as an active ingredient a mixed extract obtained by extracting natural vegetable products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger with hot water, and then extracted with ethanol . delete delete A health functional food for preventing or improving diabetes, comprising a mixed extract obtained by extracting natural vegetable products including parsley, burdock, pumpkin, plantain leaves, red beans, black beans, jujube and ginger with hot water, and then extracted with ethanol.

Images