KR20110090443A - Cosmetic composition containing omandungui glycosaminoglycan extracts for skin regeneration - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a glycosaminoglycan composite is provided to ensure skin regeneration effect without skin irritation and to suppress skin aging by moisturizing. CONSTITUTION: A cosmetic composition 0.01-30 weight% of glycosaminoglycan isolated from Styela plicata. The glycosaminoglycan composite is prepared by heating Styela plicata in 1-10 times of water at 100-130°C for 2-5 hours, treating with 50-80% of ammonium persulfate to remove proteins, and treating with 3-7 times of alcohol to dry precipitate. The composition is manufactured in the form of lotion, nutrition cream, massage cream, massage pack, or foundation of emulsifying type.

Description

         Cosmetic composition containing omandungui glycosaminoglycan extracts for skin regeneration}

The present invention relates to a skin regenerating cosmetic composition containing a glycosaminoglycan complex extracted from an oar, and cosmetics using marine life include chitin chitosan using crustaceans among marine life and cosmetic materials using marine invertebrates. One that has recently gained attention is glycosaminoglycans.

Glycosaminoglycans are acidic mucopolysaccharides contained in the connective tissues of animals and consist of a repeating structure of D-glucouronic acid and disaccharides of sulfated N-acetylgalactosamine. There are many kinds of different isomers. Glycosaminoglycans include hyaluronic acid, dermatan sulfate, chondroitin sulfate, and heparin according to these structural differences. Proteoglycans, including chondroitin sulfate, have been used as food additives and medicines because they have been found to prevent and treat degenerative arthritis, to prevent aging and antitumor. Chondroitin sulfate has the property of accumulating moisture and nutrients in skin, organs, muscles, etc., and can be an excellent material that can delay skin aging by inhibiting the decrease of skin moisture and nutrient content that causes skin aging.

(Document 1) Method for preparing sea cucumber extract containing chondroitin sulfate (Korea Patent 0167831)

(Document 2) Extraction and Purification of Sulfate Polysaccharides from Off-White Peel (Korean Patent 10-0229948)

As a feedstock for chondroitin sulfate, most products are extracted from cartilage of terrestrial mammals such as cattle and pigs, but they are avoided by consumers due to diseases such as mad cow disease and foot and mouth disease. For this reason, many studies have been conducted to obtain glycosaminoglycans from other materials. Thus, the present inventors have attempted to develop glycosaminoglycans contained in the nests of the marine invertebrates produced in Korea as a sample and develop them as raw materials for anti-aging cosmetics.

Therefore, the present inventors grow in the clean area of the south coast of Korea, the growth rate is very fast, the production volume is high, and the price is also cheap compared to other marine invertebrates. Glycosaminoglycans were extracted from these halves.

It is an object of the present invention to provide a novel use of glycosaminoglycan complexes extracted from halves of marine invertebrates.

In another aspect, it is an object of the present invention to provide a cosmetic composition containing a glycosaminoglycan complex extracted from a snag as an active ingredient.

In another aspect, an object of the present invention is to provide a cosmetic composition having a skin regeneration, moisturizing effect.

As described above, Omani GAG extract prepared by the present invention is excellent in skin regeneration effect, excellent skin moisturizing effect and exhibits the effect of delaying skin aging. In addition, Omani GAG extract from Tongyeong, a clean area of the south coast, has excellent skin stability and has no merit for skin.

         The present invention extracts the glycosaminoglycan complex from the snag and uses the extract as a cosmetic composition. As used herein, the term "glycosaminoglycan complex" refers to a substance comprising glycosaminoglycan and other substances. In the present invention, other substances are substances consisting of "saccharides, proteins and ash" and the like.

Glycosamino complexes extracted from the halves may be included in an amount of 0.1 to 30% by weight based on the total weight of the composition. When the content of the glycosamino glycan complex is less than 0.01% by weight, there may be a problem in that a skin regeneration effect, a moisturizing effect, etc. are hardly shown. If it exceeds 50% by weight, a problem may arise that the manufacture of cosmetics is not suitable.

The cosmetic composition of the present invention may be prepared in any form of formulation commonly prepared in the art. For example, the formulation may be selected from the group consisting of skin cosmetics based on flexible skin lotion, astringent lotion, nourishing lotion, nourishing cream, eye cream, massage cream, essence, powder and pack, but is not limited thereto. It is not.

 Omani purchased from Tongyeong Central Market was washed with running water until clear water came out. The sample was placed in a high pressure steam cooker (Dongnam Industrial Co., DN-1001) and water 5-10 times the sample volume was added thereto. Then, the mixture was cooked for 2 to 5 hours at a temperature of 100 to 130 ° C. It was allowed to stand at room temperature and then centrifuged at 3,000 rpm for 20 minutes (Hanil Scentific Industrial, MF 600 PLUS, Large Capacity Centrifuge). The settled precipitate was discarded and the supernatant was taken and concentrated with a rotary evaporator (Hahn Shin Scentific, Model No. 20sp) until brix 10. 50-80% ammonium sulphate was added to remove the contained protein and left for 24 hours in refrigerated condition (0-10 ° C). It was centrifuged at 3,000 rpm for 20 minutes and filtered. The supernatant was dialyzed with distilled water until the salt content was less than 0.1%. This solution was concentrated to Brix 10. Three to seven times the concentrated amount of alcohol was added to precipitate glycosaminoglycans. The precipitate was centrifuged at 3,000 rpm for 20 minutes to be lyophilized (FDU-540, Tokyo Rikakikai Co., Ltd, Tokyo, Japan) to obtain Oman glycosaminoglycan extract. The yield of Omani glycosaminoglycan complex obtained through this process was 0.80%.

The content of glycosaminoglycans in the extract was about 10% according to the Carbazole assay, a method of measuring and converting uronic acid content according to the Food Code.

(Example)

Preparation of Serum Containing Glycosaminoglycans (hereinafter referred to as GAG) Extracted from Omanis

2 g and 3 g of Omani GAG lyophilisate prepared by the above method were added to 68 ml of 67 ml of purified water, respectively, and were dissolved at 65 ° C. at 350 rpm. 10 g of propylene glycol and 20 g of glycerin were added to the above solution, and a preservative (Gsilk-P350, GFC Co. Ltd, Korea) was added thereto and dissolved at 40 to 60 ° C. for 20 minutes at 1,200 rpm. After standing at room temperature, the filtrate filtered with 0.25um filter paper was used as 2% and 3% glycosaminoglycan cosmetic liquid.

 Production example of Omani GAG essence ingredient Control group Example 1
(2% GAG serum) Example 2
(3% GAG serum) end extract 0g 2 g 3g Purified water 70ml 68 ml 67 ml I Propylene glycol 10g 10g 10g glycerin 20g 20g 20g antiseptic A reasonable amount A reasonable amount A reasonable amount Sum 100 ml 100 ml 100 ml

(Test Example 1)

- toxicological testing of samples

To determine the toxicity of the sample, a toxicity test was conducted with RAW 264.7 cells. To determine the toxicity, Omani GAG complexes were prepared in medium at final concentrations of 1 ug / ml, 50 ug / ml, 100 ug / ml and 200 ug / ml, respectively. Rather, it is expected that the growth of the cells may be increased to promote the growth of the cells, thereby contributing to the regeneration effect of the skin cells, compared to the control without the sample.

(Test Example 2)

- Playback effects of skin fibroblasts

 Human dermal fibroblast cells (CCD986sk) were purchased from Korea Cell Line Bank and cultured with DMEM (Gibco) medium containing 15% calf serum (FBS; Gibco). The cells were dispensed at 4000 cells / well in 96-well plates, and after 24 hours, GAGs extracted from the hounds were treated with cell culture medium containing 10% FBS. Incubate for 7 days, replacing the medium containing the sample once every 2-3 days, and then determine the proliferation rate of the cells by MTS [3- (4,5-dimethyl-thiazol-2-yl) -5-carboxy-methoxyphenyl-2 -(4-sulfophenyl) -2H-tetrazolium] reagent was treated for 1 hour and absorbance was measured at 490 nm with a microplate reader (Perkin Elmer 1420 Multilabel Counter VICTORTM, USA). Cell viability was expressed as absorbance of the sample treated group compared to the control.

 Proliferative Effect of Oman GAG Extract sample Proliferation rate (%) Control 100 ± 4.3 GAG 1 ug / ml 103.2 ± 2.9 GAG 50 ug / ml 104.3 ± 4.7 GAG 100 ug / ml  114.7 ± 7.5 * GAG 200 ug / ml   131.2 ± 5.4 **

* p <0.5, ** p <0.01

(Test Example 3)

- measurement of skin moisturizing effect

We measured the moisturizing effect of GAG on 10 men and women with healthy skin at room temperature 22 ~ 24 ℃, relative humidity 55% and no air flow. First, the details were marked with a circle of 2.5cm in diameter on the arm of the subject's wrist and the skin moisture content was measured before sample application. After the measurement, each of the marked areas was coated with a control group containing no hummingbird GAG, 2% cosmetic liquid containing hummingbird GAG and 40ul of 3% unsolvent. Immediately after application, 30 minutes later, and 1 hour later, 2 hours later, the water content of the skin was measured. The moisture content of the skin was measured using the skin diagnostic device Aramo-Ts (Aramhuvis, Korea) to measure the persistence of the moisturizing effect.

 Moisturizing Benefits of Omani GAG Application time sample Moisture content percentage(%) Before application Control 26.0 ± 0.9 100.0 ± 3.4 2% GAG Serum 25.7 ± 0.8 100.0 ± 3.1 3% GAG Serum 26.2 ± 1.0 100.0 ± 3.8 Immediately after application Control 43.2 ± 6.6 166.0 ± 25.4 ** 2% GAG Serum 41.7 ± 4.7 162.3 ± 18.1 ** 3% GAG Serum 42.2 ± 5.4 161.1 ± 20.7 ** 30 minutes after application Control 27.0 ± 1.6 103.8 ± 6.3 2% GAG Serum 28.0 ± 1.8 109.1 ± 7.0 * 3% GAG Serum 29.0 ± 1.8 110.8 ± 7.0 * 1 hour after application Control 26.8 ± 1.3 102.9 ± 4.8 2% GAG Serum 26.8 ± 0.5 104.2 ± 1.9 * 3% GAG Serum 28.3 ± 1.5 108.0 ± 5.8 * 2 hours after application Control 26.2 ± 1.6 100.6 ± 6.2 2% GAG Serum 26.8 ± 0.4 102.6 ± 4.7 3% GAG Serum 27.8 ± 1.2 106.4 ± 4.5 *

* p <0.5, ** p <0.01

(Test Example 4)

Skin irritation test

Human patch test was performed to investigate the skin irritation of the GAG serum. Ten healthy men and women were patched with control and 2% and 3% GAG for 24 hours. After 24 hours of patching it was removed and skin irritation measured and skin irritation was also measured after 24 and 48 hours. According to the results, it was proved that the skin irritation of the gigantic GAG essence liquid according to the present invention was very insignificant.

 Skin irritation test of GAG sample The number of the subjects When to investigate Judgment result Remarks ++ + - Control 10 people Immediately after removal - - 10 10 people 24 hours later - - 10 10 people 48 hours later - - 10 Preparation Example 1 10 people Immediately after removal - One 9 10 people 24 hours later - - 10 10 people 48 hours later - - 10 Preparation Example 2 10 people Immediately after removal - One 9 10 people 24 hours later - One 9 10 people 48 hours later - 0 10

++ badly reddened

 + Slightly reddish

-No response

Claims (5)

Cosmetic composition, characterized in that extracted from the invertebrates of marine invertebrates put water corresponding to 1 ~ 10 times of the sample in the extractor and heated for 5 hours at 100 ~ 130 ℃ 2 hours
Cosmetic composition containing the one obtained by treating with 50-80% ammonium sulfate to remove protein from the extract of claim 1
Cosmetic composition containing the obtained by drying the precipitate produced by the process of Claims 1 and 2 by 3-7 times alcohol.
Cosmetic composition, characterized in that it comprises 0.01 to 30% by weight of the gigantic glycosaminoglycan extract based on the total weight of claim 1, 2, 3
The cosmetic composition is a cosmetic composition characterized in that the formulation of the lotion, nourishing lotion, nourishing cream, massage cream, nourishing essence, massage pack or emulsified foundation (can I have no content?)