KR101525876B1 - Method for making glue from donkey skin and cosmetics containing the same - Google Patents

Abstract

The present invention relates to a method for manufacturing glue from donkey skin and a cosmetic composition comprising the glue as an active ingredient and, more specifically, to a technique for manufacturing a cosmetic product for skin improvement using the same, regarding a manufacture of glue by extracting donkey skin, using seashell powder during an extraction process to increase a glue production rate, and manufacturing a glue which is excellent in skin moisture, relief of inflammatory skin, and regenerative activity of skin cells.

Description

TECHNICAL FIELD The present invention relates to a method for producing a glue from a donkey skin and a cosmetic composition containing the glue from a donkey skin and cosmetics,

The present invention relates to a method for producing a glue from a donkey's skin and a cosmetic composition containing the glue as an active ingredient. More particularly, the present invention relates to a cosmetic composition comprising glue shell powder The present invention relates to a technique for producing a glue which is excellent in skin moisturizing, skin irritation alleviation and skin cell regeneration activity, and which is used in the production of cosmetics for skin improvement.

The function of the skin is degraded due to external factors such as ultraviolet rays, environmental pollution, aging caused by internal factors such as age increase and stress. Among these, ultraviolet rays cause wrinkles of skin, decrease of elasticity, decrease of skin moisture, and the like, which causes symptoms such as loss of skin flexibility, skin dryness, and skin barrier weakness. In addition, such skin damage is accompanied by skin inflammation, and when a skin inflammation reaction occurs, gene mutation and nerve damage in related cells are promoted to promote an inflammatory reaction.

In studying various substances having anti-inflammatory activity, the present inventors studied the ingredients contained in the donkey skin. The present inventors have found that the donkey skin contains the glutinous component having superior skin moisturizing effect, Regenerating effect and the like, and thus it can be used as a moisturizing and anti-inflammatory cosmetic. The present inventors have further found that when using the shell shell powder during the extraction process in extracting the glue from the donkey shell through the additional study, the yield is increased and the skin moisturizing, skin irritation mitigation and skin cell regeneration activity are increased Thus completing the present invention.

Korean Patent Laid-Open Publication No. 2014-0059479 entitled " Method for producing functional gelatin concentrate using donkey bark extract and donkey bark extract, "discloses the use of the donkey bark extract, glue as a food product, and its use as a cosmetic product is disclosed It is not.

The object of the present invention is to provide a method for producing a glue having increased skin-protecting activity and skin-protecting activity by using a shell-shell powder in preparing a glue by extracting donkey skin.

Another object of the present invention is to provide a cosmetic composition containing a glue component which is produced by the above-described method and is excellent in skin moisturizing, skin irritation alleviation, and skin cell regeneration.

According to an aspect of the present invention, there is provided a donkey skin pretreatment method comprising the steps of: (A) removing donkey skin fines and foreign substances and removing a donkey smell; (B) at least one clamshell selected from the group consisting of abalone shells, oyster shells, shell shells, carrot shells, scallop shells and pearl shells are heated at 800 to 1500 DEG C for 2 to 5 hours, Peel powder manufacturing step; (C) mixing the donkey shell and the shell shell powder at a weight ratio of 2: 4: 1, stirring and heat-treating the mixture; And (D) extracting the mixture to prepare a glue.

At this time, it is more preferable that the shell shell powder is a pearl shell powder.

The heat treatment is preferably performed by mixing the donkey shell and the shell shell powder and stirring at 80 to 100 ° C for 15 to 30 minutes.

The extraction is hot water extraction, supercritical extraction or steam extraction, more preferably steam extraction.

According to another aspect of the present invention, there is provided a cosmetic composition comprising 0.1 to 10 wt% of a glue prepared by the above-described method, based on the total weight of the cosmetic composition.

The cosmetic composition can be used for skin wrinkle improvement, skin moisturization, skin inflammation relief, and skin regeneration.

The glue prepared according to the present invention has a high yield and is excellent in skin moisturizing, skin irritation and skin cell regeneration effects, and is useful for improving skin wrinkles, skin moisturizing, skin irritation, and skin regeneration .

Hereinafter, the present invention will be described in more detail.

Donkeys have spread all over the world and are distributed in Africa, Central America, and Asia, and have been used primarily as transportation for children and women. Donkeys are easy to breed because they have a high survival rate even in the climatic conditions. They are resistant to diseases and are registered as livestock products in Korea. According to Donguibogam, the effects of aging and bone health on donkey bone and leather extracts are explained.

On the other hand, clamshells are not separated into food waste, so there are many difficulties in the treatment process. If they are treated by incineration or the like, they cause environmental pollution due to dust or harmful gas emission. However, the calcium carbonate contained in the shell-shell powder has a sterilizing ability and the useful ingredient contained in the shell-shell powder, among which conchiolin is known to promote blood circulation.

The present invention is characterized not only by increasing the yield of glue by using the shell shell powder in the preparation of glue from the donkey shell, but also by increasing the skin protection activity of the glue itself.

According to one embodiment of the present invention, glue is manufactured by the following manufacturing method.

(A) a pretreatment step of a donkey shell to remove foul hair and foreign matter from the donkey's skin and remove the donkey's smell; (B) at least one clamshell selected from the group consisting of abalone shells, oyster shells, shell shells, carrot shells, scallop shells and pearl shells are heated at 800 to 1500 DEG C for 2 to 5 hours, Peel powder manufacturing step; (C) mixing the donkey shell and the shell shell powder at a weight ratio of 2: 4: 1, stirring and heat-treating the mixture; And (D) extracting the mixture to prepare a glue.

First, before extracting the donkey skin, remove fine hairs and foreign matter, and perform pretreatment to remove the smell of the donkey. After pretreatment, cut into appropriate size and store in refrigerator.

The shell shell powder is prepared by heating the shell shell at 800 to 1500 ° C. for 2 to 5 hours and then pulverizing the shell shell. There is no limitation to the shell used at this time, but it is more preferable to use the shell of pearl shell. The effect of removing the adhering substances attached to the pearl shell was most excellent when the shell layer and the pearl layer were remained to prepare the shell shell powder.

Next, the prepared donkey shell and shell shell powder are mixed and heat-treated. The donkey shell and the shell shell powder are mixed at a weight ratio of 2: 4: 1, more preferably 2: 1, and heat-treated while stirring. According to one embodiment of the present invention, the donkey shell prepared by cutting to a suitable size of 2 to 5 cm in width and length, and the shell shell powder are mixed and stirred at 80 to 100 ° C for 15 to 30 minutes while stirring.

The mixture thus heat-treated is extracted. Extraction with hot water, extraction with solvent, supercritical extraction or steam extraction, and it is more preferable to perform the extraction by the steam extraction method. The solvent may be at least one selected from the group consisting of water, ethanol, methanol, butanol, propanol, butylene glycol, glycerin, chloroform, ethyl acetate, dichloromethane, hexane, acetone, acetonitrile, petroleum ether and diethyl ether Can be used.

The glue component thus prepared exhibited a superior glutathione production rate (Test Example 1) as compared with the conventional hot-water or solvent extract due to synergy caused by the pearl shell powder during the mixing and heat-treatment of the donkey shell and the pearl shell- , Increase of collagen expression (Test Example 2), increase of Hyaluronan Synthase-3 expression (Test Example 3), increase of Fliaggrin expression (Test Example 4), increase of Aquaporin-3 expression (Test Example 5) (Test Example 6), a skin cell regeneration effect (Test Example 7), and a skin irritation alleviating effect (Test Example 8).

The glue as the active ingredient is contained in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. The cosmetic composition containing the glue component extracted from the donkey skin of the present invention can be manufactured into any of the commonly used formulations, and examples thereof include lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, Oil, body gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, mascara, make-up base and the like.

[Example]

Hereinafter, the present invention will be described in detail by way of the following examples and test examples, but the present invention is not limited to these examples.

Preparation Example 1: Pretreatment of donkey shell

The donkey shell was used in Taesan Don Kipam in Yongin, Gyeonggi Province. After removing the fine hairs and foreign matter from the donkey shell, the flour and coarse salt were mixed 1: 1 and washed three times to remove the donkey smell. 2 kg of donkey skin was cut into 2 cm in width and 2 cm in length and stored in a refrigerator.

Comparative Example 1: Production of hot-water extract of glue component from donkey skin

200 g of the pretreated donkey shell was mixed with 1000 g of a mixed solvent of ethanol and purified water at a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and extracted with an extractor equipped with a cooling condenser at 100 DEG C for 8 hours. After filtration using a filter paper (Qualitative Filter Papers No. 2), the filtrate was concentrated under reduced pressure at 60 ° C or lower to obtain 4.2 g in powder form.

Comparative Example 2: Preparation of supercritical extract of glue component from donkey skin

200 g of the pretreated donkey shell was filled into an extractor of an SC-CO ₂ extraction system (Ilshin Autoclave Co., Ltd., Korea), and then carbon dioxide, which is a saturated pressure state, . At this time, temperature and pressure conditions were fixed at 40 ° C and -400 bar and then extracted for 1 hour. The mixture was extracted and put in a vacuum concentrator to remove residual carbon dioxide to obtain 8 g in powder form.

Comparative Example 3: Preparation of glutinous steam extract from donkey skin

200 g of the pretreated donkey shell was mixed with 1000 g of a mixed solvent of ethanol and purified water at a ratio of 3: 7 (w / w), placed in an inner container of the extractor, placed in an outer container filled with water, And then extracted with high temperature and high pressure steam. After filtration using a filter paper (Qualitative Filter Papers No. 2), the filtrate was concentrated under reduced pressure at 60 ° C or less to obtain 6 g in powder form.

Production Example 2: Preparation of pearl shell powder

The pearl shell was heated at 800 ° C for 2 hours and pulverized to prepare a shell shell powder. The adhered material on the shell was removed, and the shell layer and the pearl layer were left to produce the shell shell powder.

Example 1: Preparation of hot water extract of glue component from donkey skin

The donkey shell of Preparation Example 1 and the pearl shell shell powder of Preparation Example 2 were mixed and the donkey shell (200 g) and the pearl shell shell powder (100 g) were mixed at a weight ratio of 2: 1 and stirred at 80 캜 for 15 minutes Heat treated. After the heat treatment, the mixture was mixed with 1000 g of a mixed solvent of ethanol and purified water at a ratio of 3: 7 (w / w). The mixture was immersed for 3 days and extracted with an extractor equipped with a cooling condenser at 100 DEG C for 8 hours. After filtration using a filter paper (Qualitative Filter Papers No. 2), the filtrate was concentrated under reduced pressure at 60 ° C or lower to obtain 16 g in powder form.

Example 2: Preparation of supercritical extract of glue component from donkey skin

The donkey shell of Preparation Example 1 and the pearl shell shell powder of Production Example 2 were mixed and the donkey shell (200 g) and the pearl shell shell powder (100 g) were mixed at a weight ratio of 2: 1 and stirred at 80 캜 for 15 min Lt; / RTI > After the heat treatment, it is filled in an extractor of supercritical fluid extractor (SC-CO ₂ extraction system, Ilshin Autoclave Co., Ltd., Korea), and then carbon dioxide in a saturated pressure state is injected into the apparatus. At this time, temperature and pressure conditions were fixed at 40 ° C and -400 bar and then extracted for 1 hour. After the extraction, it was put in a vacuum concentrator to remove residual carbon dioxide to obtain 14.2 g in powder form.

Example 3: Preparation of glucomannan steam extract from donkey skin

The donkey shell of Preparation Example 1 and the pearl shell shell powder of Production Example 2 were mixed and the donkey shell (200 g) and the pearl shell shell powder (100 g) were mixed at a weight ratio of 2: 1 and stirred at 80 캜 for 15 min Lt; / RTI > After heat treatment, it was mixed with 1000g of mixed solvent of ethanol and purified water at a ratio of 3: 7 (w / w), placed in an inner container of the extractor, placed in an external container filled with water, completely sealed and then subjected to high temperature and high pressure steam treatment at 130 ° C for 3 hours Respectively. After filtration using a filter paper (Qualitative Filter Papers No. 2), the filtrate was concentrated under reduced pressure at 60 ° C or lower to obtain 20 g in powder form.

Test Example 1: Measurement of glue yield

The donkey bark extract prepared by the method of the present invention was dried and the yield of glue was measured.

Name of sample Yield (%) Comparative Example 1 2.1 Comparative Example 2 4 Comparative Example 3 3 Example 1 8 Example 2 7.1 Example 3 10

As can be seen from the above Table 1, the overall yields of Examples 1 to 3 of the present invention were high.

Test Example 2: Confirmation of Increasing Effect of Collagen Expression

Fibroblast (CCD-986Sk) was adjusted to 5 × 10 ⁵ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After culturing, the samples of the above Examples and Comparative Examples were treated and cultured for 24 hours. RNA was extracted from the cultured cells and the increase rate of collagen expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The increase rate of collagen expression was calculated by the following formula, and the results are shown in Table 2 below. As a control group, adenosine was used.

Collagen expression rate (%) = (sample collagen expression level / control collagen expression level) × 100

Name of sample Treatment concentration (w / v%) Collagen expression rate (%) Comparative Example 1 0.5 81.7 Comparative Example 2 0.5 80.9 Comparative Example 3 0.5 82.5 Example 1 0.5 86.9 Example 2 0.5 85.7 Example 3 0.5 91.1 Adenosine 0.05 83.8

As shown in Table 2, the donkey bark extracts of Examples 1 to 3 exhibited better collagen expression than the donkey bark extracts of Comparative Examples 1 to 3 at the same concentration, and showed equivalent or superior effects compared with adenosine Respectively. In particular, Example 3 showed the most excellent effect.

Test Example 3: Confirmation of Increase of Hyaluronan Synthase-3 Expression

Fibroblast (CCD-986Sk) was adjusted to 5 × 10 ⁵ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After culturing, the samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells and the expression of Hyaluronan Synthase-3 (HAS-3) was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The HAS-3 expression increase rate was calculated by the following equation, and the results are shown in Table 3 below. As a control group, hyaluronic acid was used.

HAS-3 expression ratio (%) = (expression amount of sample HAS-3 / expression amount of control HAS-3) × 100

Name of sample Treatment concentration (w / v%) Hyaluronan Synthase-3 Expression Rate (%) Comparative Example 1 0.5 81.4 Comparative Example 2 0.5 79.6 Comparative Example 3 0.5 82.4 Example 1 0.5 82.9 Example 2 0.5 86.7 Example 3 0.5 90.4 Hyaluronic acid 0.05 87.8

As shown in Table 3, the donkey bark extracts of Examples 1 to 3 exhibited a superior effect on hyaluronan Synthase-3 expression compared to the donkey bark extracts of Comparative Examples 1 to 3 at the same concentration treatment, Or showed excellent effect. In particular, Example 3 showed the most excellent effect.

Test Example 4: Confirmation of Increasing Effect of Filaggrin Expression

Fibroblast (CCD-986Sk) was adjusted to 5 × 10 ⁵ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After culturing, the samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells and the increase rate of filaggrin expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The increase rate of filaggrin expression was calculated by the following equation, and the results are shown in Table 4 below. As a control group, hyaluronic acid was used.

Filaggrin Expression Rate (%) = (sample filaggrin expression amount / control filaggrin expression amount) × 100

Name of sample Treatment concentration (w / v%) Filaggrin Expression Rate (%) Comparative Example 1 0.5 80.7 Comparative Example 2 0.5 83.4 Comparative Example 3 0.5 82.9 Example 1 0.5 84.5 Example 2 0.5 87.6 Example 3 0.5 92.7 Hyaluronic acid 0.05 85.8

As shown in Table 4, the donkey bark extracts of Examples 1 to 3 exhibited better filaggrin expression than the donkey bark extracts of Comparative Examples 1 to 3 at the same concentration treatment, and showed equivalent or superior effects compared to hyaluronic acid Respectively. In particular, Example 3 showed the most excellent effect.

Test Example 5: Confirmation of Increase in Aquaporin-3 Expression

Fibroblast (CCD-986Sk) was adjusted to 5 × 10 ⁵ cells / well using IMDM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After culturing, the samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells and reverse transcription polymerase chain reaction (RT-PCR) was performed to confirm the increase of expression of Aquaporin-3. The increase rate of Aquaporin-3 expression was calculated by the following equation, and the results are shown in Table 5 below. As a control group, hyaluronic acid was used.

Expression rate of Aquaporin-3 (%) = (amount of expressed Aquaporin-3 / amount of expressed Aquaporin-3 of control) × 100

Name of sample Treatment concentration (w / v%) Aquaporin-3 Expression Rate (%) Comparative Example 1 0.5 82.5 Comparative Example 2 0.5 84.6 Comparative Example 3 0.5 83.3 Example 1 0.5 84.5 Example 2 0.5 85.5 Example 3 0.5 92.4 Hyaluronic acid 0.05 88.6

As shown in Table 5, the donkey bark extracts of Examples 1 to 3 exhibited better aquaporin-3 expression than the donkey bark extracts of Comparative Examples 1 to 3 at the same concentration treatment, It showed equal or superior effect compared with Lonic acid. In particular, Example 3 showed the most excellent effect.

Test Example 6: Confirmation of TNF-α expression inhibitory effect

RAW 264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% FBS, and then inoculated on a 12-well plate and cultured for 18 hours. After incubation, starvation was maintained for 12 hours, LPS 10 ng / mL and samples were treated and incubated for 24 hours. RNA was extracted from the cultured cells and the inhibition rate of TNF-α expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR). The inhibition rate of TNF-α expression was calculated by the following equation, and the results are shown in Table 6 below. Dexamethasone was used as a control.

Inhibition rate (%) of TNF-α expression = 100 - [(expression amount of sample TNF-α / expression amount of control group TNF-α) × 100]

Name of sample Treatment concentration (w / v%) TNF-α expression inhibition rate (%) Comparative Example 1 0.5 75.9 Comparative Example 2 0.5 80.4 Comparative Example 3 0.5 82.5 Example 1 0.5 84.3 Example 2 0.5 85.3 Example 3 0.5 88.7 Dexamethasone 0.05 85.4

As shown in Table 6, the donkey bark extracts of Examples 1 to 3 showed an excellent TNF-α inhibitory effect compared to the donkey bark extracts of Comparative Examples 1 to 3 at the same concentration, and were equivalent to dexamethasone And showed excellent effects. In particular, Example 3 showed the most excellent effect.

Test Example 7: Confirmation of skin cell regeneration effect

A wound healing assay was performed to confirm the skin regeneration effect of the donkey bark extract. The keratinocyte (HACAT) was adjusted to 5 × 10 ⁵ cells / well using DMEM supplemented with 10% FBS, and then inoculated on a 6-well plate and cultured for 18 hours. The cells grown on the 6 well plate were scratched to remove a part of the cells from the bottom of the 6-well plate, and the samples of the above-mentioned Examples and Comparative Examples were treated and cultured for 18 hours. The degree of cell regeneration was confirmed, and the results are shown in Table 7 below.

Cell regeneration was calculated by the following equation, and dexamethasone was used as a control.

Cell regeneration rate (%) = (sample cell regeneration / control cell regeneration) x 100

Name of sample Treatment concentration (w / v%) Skin cell regeneration (%) Comparative Example 1 0.5 80.1 Comparative Example 2 0.5 83.2 Comparative Example 3 0.5 84.6 Example 1 0.5 88.5 Example 2 0.5 88.4 Example 3 0.5 92.7 Dexamethasone 0.05 85.2

As shown in Table 7, the donkey bark extracts of Examples 1 to 3 exhibited superior skin cell regeneration effects compared to the donkey bark extracts of Comparative Examples 1 to 3 at the same concentration treatment, and showed equivalent or superior effects compared with dexamethasone Respectively. In particular, Example 3 showed the most excellent effect.

Example 4: Production of a cream containing a glue component obtained from donkey skin

The creams containing the glue components obtained from the donkey shells prepared in the above examples were prepared according to the conventional methods with the compositions and contents shown in Table 8 below.

(weight%) Control Example Example 4 Donkey skin steam extract (Example 3) - 2 Ethylenediamine acetic acid, disodium salt 0.02 0.02 Guar hydroxypropyltrimonium chloride 0.1 0.1 glycerin 2.0 2.0 Panthenol 0.05 0.05 Cocamidopropyl betaine 2.0 2.0 Ammonium lauryl sulfate 5.0 5.0 Laura Maid Diai 2.0 2.0  Glycollosteralate 1.0 1.0 Sodium lauryl sulfate 15.0 15.0 Sodium lauryl sulfate (30%) 20.0 20.0 Chloride set remnium 0.5 0.5 Methylchloroisothiazolinone (1.05-1.2%) 0.01 0.01 Methylisothiazolinone (0.3 ~ 0.45%) 0.01 0.01 Magnesium chloride (0.5 to 1.0%) 0.01 0.01 Magnesium nitrate (21.0 to 23.5%) 0.01 0.01 Citric acid 0.07 0.07 Dimethicone (55 to 60%) 0.5 0.5 Laureth -3 (2-4%) 0.5 0.5 Laureth-20 (2-4%) 0.5 0.5 incense 0.15 0.15 Purified water To 100 To 100

Test Example 8: Confirmation of skin irritation mitigation effect

After 10 stimuli were induced by tape stripping method on the inner part of the lower arm of 10 men and women in their 20s and 30s who had no skin allergic symptoms, the donkey skin using the pearl shell powder according to the present invention The degree of the stimulation induced by applying the cosmetic composition containing steam extract and the cosmetic composition not containing the steam extract was evaluated relative to each other. First, taping was repeated 30 times with a scotch tape on both sides of the underbody to cause skin erythema. After erythema induction, the cosmetic composition containing the donkey skin steam extract using the pearl shell powder according to the present invention was applied and the cosmetic composition containing no glue component according to the present invention was applied. The degree of skin irritation was evaluated 3 hours after application.

≪ Evaluation of degree of skin irritation (erythema) >

0 (no response) 1 (very mild erythema) 2 (faint or mild erythema)

3 (clear erythema but weak erythema) 4 (pronounced erythema) 5 (severe erythema)

Name of sample Degree of skin irritation Left Right Control Example One One Example 4 0 0

As can be seen in Table 9, the cream composition containing the donkey skin-shell extract using the present pearl shell powder showed better skin irritation mitigation effect than the control without the donkey skin-shell extract using the pearl shell powder Respectively.

Example 5: Flexible lotion preparation

A softening lotion containing a donkey skin steam extract using the pearl shell powder in the composition shown in Table 10 below was prepared by a conventional method.

ingredient Content (% by weight) Donkey skin steam extract (Example 3) 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl vinyl polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example 6 Production of Nourishing Lotion (Milk Lotion)

A nutritional lotion containing a donkey skin steam extract using the pearl shell powder in the composition shown in Table 11 was prepared by a conventional method.

ingredient Content (% by weight) Donkey skin steam extract (Example 3) 0.5 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia nut oil 2.5 Polysorbate 20 1.6 Sorbitan sesquiterate 1.6 Propylparaben 0.6 Carboxyl vinyl polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example 7: Preparation of nutrition cream

Nutritive creams containing donkey skin steam extracts using the pearl shell powder in the compositions listed in Table 12 below were prepared by conventional methods.

ingredient Content (% by weight) Donkey skin steam extract (Example 3) 0.5 Pro-type glycerin monostearate 2.0 Stearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Purified vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Preservative, fragrance, pigment a very small amount Purified water Balance Sum 100

Example 8: Massage cream production

Massage cream containing donkey skin steam extract using pearl shell powder in the composition shown in Table 13 below was prepared by a conventional method.

ingredient Content (% by weight) Donkey skin steam extract (Example 3) 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl vinyl polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example 9: Pack manufacture

A pack containing a donkey shell steam extract using the pearl shell powder in the composition shown in Table 14 below was prepared by a conventional method.

ingredient Content (% by weight) Donkey skin steam extract (Example 3) 0.5 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 glycerin 4.5 Carbopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Balance system 100

Claims (11)

(A) a pretreatment step of a donkey shell to remove foul hair and foreign matter from the donkey's skin and remove the donkey's smell;
(B) at least one clamshell selected from the group consisting of abalone shells, oyster shells, shell shells, carrot shells, scallop shells and pearl shells are heated at 800 to 1500 DEG C for 2 to 5 hours, Peel powder manufacturing step;
(C) mixing the donkey shell and the shell shell powder at a weight ratio of 2: 4: 1, stirring and heat-treating the mixture; And
(D) extracting the mixture to prepare a glue. The method of claim 1, wherein the shell shell powder is made of pearl shell. The method of claim 1, wherein the heat treatment is performed by mixing the donkey shell and the shell shell powder with stirring, and at 80 to 100 ° C for 15 to 30 minutes. The method of claim 1, wherein the extraction is hot water extraction, supercritical extraction, or steam extraction. A cosmetic composition comprising the glue prepared by any one of claims 1 to 4 in an amount of 0.1 to 10% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 5, wherein the cosmetic composition is for improving skin wrinkles. The cosmetic composition according to claim 5, wherein the cosmetic composition is for moisturizing the skin. The cosmetic composition according to claim 5, wherein the cosmetic composition is for alleviating skin inflammation. 6. The cosmetic composition according to claim 5, wherein the cosmetic composition is for skin regeneration. The cosmetic composition according to claim 5, wherein the cosmetic composition is for alleviating skin irritation. The cosmetic composition according to claim 5, wherein the cosmetic composition is at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, Wherein the cosmetic composition has one of the formulations selected from the group consisting of shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, milky lotion, press powder, loose powder and eye shadow.