KR101415990B1 - Composition of skin external application containing green tea flower essential oil - Google Patents

Abstract

The present invention relates to an external preparation for skin containing an essential oil of green tea as an active ingredient. More specifically, the present invention relates to an external preparation for skin, which has an effect of improving wrinkles, improving elasticity and improving moisturization, It contains green tea essential oil, which protects the skin by eliminating the toxicity caused by environmental harmful factors, reduces the pigmentation, maintains the intrinsic fragrance of green tea flowers without side effects and has excellent skin effect. Skin external composition.

Green tea flower flavor, green tea essential oil, wrinkle improvement, skin elasticity improvement, moisturizing improvement, fine inflammation control, detoxification cell damage protection, pigmentation control

Description

[0001] The present invention relates to a composition for external application for skin containing green tea flower essential oil,

The present invention flower tea (Camellia sinensis there is . sinenis cv. The present invention relates to a composition for external application for skin containing essential oil as an active ingredient, and more particularly to a composition for external application for skin, which contains essential oils of green tea, including green tea flower components, the present invention relates to a composition capable of preventing cell damage, reducing inflammation of the skin to delay aging, suppressing wrinkle formation, and preventing skin elasticity from dropping.

Skin is the primary barrier of the human body and functions to protect the internal organs from external stimuli such as temperature and humidity changes, ultraviolet rays, and pollutants. However, internally, as the age increases, secretion of various hormones that regulate metabolism decreases, and the function of immune cells and the activity of cells are lowered, so that the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, , The normal function of the skin is weakened, aging is promoted, and pigmentation occurs due to the physical and chemical stimuli and stress caused by the increase of ultraviolet rays, free radicals and active oxygen due to the environmental pollution such as ozone layer destruction. In order to prevent this phenomenon and to maintain healthier and more beautiful skin, physiologically active substances obtained from various known animals, plants, microorganisms, etc. are added to cosmetics to maintain the inherent functions of the skin and activate skin cells Efforts have been made to effectively inhibit aging.

Green tea tree ( Camellia sinensis there is . sinenis cv. Shuixian and Maoxie) leaves have long been interested in green tea extracts and their components. Green tea extracts and green tea catechins are known to have many effects on skin aging. However, the value of the green tea is not known, and it is not useful for the production of green tea leaves. In recent years, green tea flowers have attracted attention as a fragrant plant with distinctive fresh fragrance, and thus, a fancy composition that reproduces the fragrance of excellent green tea flowers has been developed (Korean Patent No. 10-0645879: fragrance composition reproducing green tea flower fragrance {Perfume composition for expressing the fragrance of a green tea flower}. However, there is still no known effect on the skin's efficacy, such as improvement of skin wrinkles, improvement of elasticity, control of micro-inflammation, and skin moisturization of tea flower essential oil.

Accordingly, the present inventors have conducted studies on ingredients that protect skin from external harmful environment or ultraviolet rays and delay skin aging, and have found that when the composition contains green tea flower essential oil (essential oil) as an active ingredient in a composition for external application for skin, (ROS) and protects skin cells by controlling the micro-inflammation caused by external stimuli. By delaying skin aging, it suppresses wrinkle formation and reduction of skin elasticity, promotes the differentiation of keratinocytes, Promoting the production of factors, enhancing skin moisturization and reducing pigmentation.

It is an object of the present invention to provide a composition for external application for skin which contains green tea essential oil to inhibit skin aging, enhance skin moisturization, and improve overall skin condition.

In order to achieve the above object, the present invention provides a composition for external application for skin, which contains essential oil of green tea as an active ingredient.

In the present invention, the green tea flower essential oil (essential oil) is a fragrance component obtained by steam distillation after extracting green tea flowers, and the green tea flower fragrance component is 4,8-dimethy-1,3 (E) - Nonatriene (E-4,8-dimethyl-1,3,7-nonatriene) and lilac alcohol.

The green tea essential oil is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition.

In the present invention, the composition may be characterized by removing reactive oxygen species (ROS).

The present invention also provides a skin external composition for preventing skin aging comprising green tea essential oil (an essential oil) as an active ingredient.

In addition, the composition for external application for skin containing green tea flower essential oil according to the present invention has effects of prevention of skin aging, prevention and improvement of skin inflammation, prevention and improvement of skin wrinkle formation, improvement of skin elasticity, skin whitening and skin moisturizing.

The composition for external application for skin containing essential oil of green tea of the present invention protects skin cells, improves wrinkles, suppresses skin elasticity, inhibits pigmentation and so on, is superior in delaying skin aging, The effect of strengthening is excellent.

The composition for external application for skin of the present invention contains green tea essential oil (essential oil) as an active ingredient.

The essential oil of green tea used in the present invention is a green tea flower extract containing the fragrance component of green tea flower essential oil. It is an oil component obtained by steam distillation after extracting green tea flower, and compared with other essential oils, 4,8- Which provides a unique fragrance and efficacy containing E-1, 3 (E), E-4,8-dimethyl-1,3,7-nonatriene and lilac alcohol Essential oil).

In addition to the above two specific components, the essential oil of green tea is selected from the group consisting of acetophenone, alpha-methylbenzyl alcohol, 2-phenylethyl alcohol and the like, which are also contained in rose essential oil and geranium oil. alcohol, benzyl alcohol, and the like.

However, the essential oil of green tea in the present invention is not particularly limited in its extraction method for the purpose of using green tea flowers, and it is not necessary to use the green tea flower essential oil (essential oil) It can change according to purpose.

720g of green tea flowers bloomed in Seo-kwangdae in Jeju Island were divided into three 1-liter Erlenmeyer flasks, and then 1.5 liters of ethyl ether (Ethyl Ether, purity 99.0%, Fisher Scientific) was poured and immersed for 4 hours and 15 minutes . The precipitate was filtered with Advan Batch No. 2 filter paper (Advantec No. 2, 185 mm in diameter) and the flower was discarded. The extract was washed with 100 g of anhydrous sodium sulfate (Na ₂ SO _{4 ,} sodium sulphate, purity 99.7%, Sigma Aldrich) And then filtered using filter paper No. 2 of Ad-Bantech. Then, the solvent ethyl ether was volatilized in a water bath at 38 ° C to obtain a residue. 100 ml of distilled water was added to the residue, and the mixture was further subjected to steam distillation under reduced pressure while maintaining the temperature at 30 ° C to obtain 13.2 mg of a perfume concentrate. The fragrance concentrate was placed in an uncontaminated glass bottle for analysis and the lid was closed and sealed and stored in the refrigerator.

The solvent ethyl ether was volatilized and a residue was obtained. 100 ml of distilled water was added to the residue, and the mixture was further subjected to steam distillation under reduced pressure while maintaining the temperature at 30 ° C to obtain 13.2 mg of a perfume concentrate.

In order to obtain the desired effect of the present invention, the green tea essential oil is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition. When the content of the component is less than 0.0001% by weight, the action as an active ingredient is difficult to expect. When the content exceeds 30.0% by weight, there is a problem in formulation stability.

According to the present invention, the composition for external application for skin containing green tea flower essential oil has an effect of improving the skin condition by removing reactive oxygen species (ROS) generated by ultraviolet rays and detoxifying it.

In addition, the composition for external application for skin containing green tea flower essential oil according to the present invention has effects of prevention of skin aging, prevention and improvement of skin inflammation, prevention and improvement of skin wrinkle formation, improvement of skin elasticity, skin whitening and skin moisturizing.

The composition for external application for skin according to the present invention is not particularly limited in its formulation. For example, cosmetic compositions having formulations of softening longevity, nutritional lotion, massage cream, nutritional cream, pack, gel, essence or skin- Or a transdermal dosage form such as a lotion, ointment, gel, cream, patch or spray.

Further, in the respective formulations of the composition for external application for skin, other components other than the above-mentioned essential components may be selected and mixed without difficulty by those skilled in the art depending on the formulation or purpose of use of other external preparation for skin.

Hereinafter, the present invention will be described in more detail with reference to Test Examples and Examples.

[Referential Example 1] Production of green tea essential oil (essential oil) and identification of components

To extract green tea essential oil, 720 g of green tea flowers bloomed in Seo Kwangdae in Jeju Island were divided into three 1-liter Erlenmeyer flasks. Ethyl ether (purity: 99.0%, Fisher Scientific) was poured and immersed for 4 hours and 15 minutes. The precipitate was filtered with Advan Batch No. 2 filter paper (Advantec No. 2, 185 mm in diameter) and the flower was discarded. The extract was washed with 100 g of anhydrous sodium sulfate (Na ₂ SO _{4 ,} sodium sulphate, purity 99.7%, Sigma Aldrich) After filtration, the filtrate was filtered using filter paper No. 2 of Adbatech Tech. Then, ethyl ether as a solvent was volatilized in a water bath at 38 캜, and further subjected to steam distillation under reduced pressure to obtain 13.2 mg of a perfume concentrate. The fragrance concentrate was placed in an uncontaminated glass bottle for analysis and the lid was closed and sealed and stored in the refrigerator.

[Reference Example 2] Preparation of green tea leaf extract

One kilogram of green tea leaves obtained from Seokwangsaewon, Jeju Island was extracted with 5L of hexane or similar organic solvent, and the organic solvent layer was separated and removed. The above process was repeated 3 times or more to obtain a green tea leaf completely removed from the oil. After the oil-removed green tea leaves were added with an organic solvent (ethanol, methanol, butanol, ether, ethyl acetate, croform, etc.) containing water and water or 5 L of organic solvent, the mixture was extracted three times at room temperature, Lt; / RTI > The extract was filtered twice with Advanetech No. 2 filter paper (Advantec No. 2, 185 mm in diameter), and the filtrate was concentrated in a vacuum concentrator maintaining a temperature of 40 ° C or lower 89 g of dry solid were obtained.

[Reference Example 3] Preparation of green tea flower extract

To extract green tea flower extract, 1Kg of green tea flowers bloomed in Seo Kwangdae in Jeju Island were poured with 3L of ethanol or similar organic solvent and immersed for 15 hours. The precipitate was filtered through filter paper No. 2 (Advantec No. 2, 185 mm in diameter), and the flower was discarded. The filtrate was concentrated in a vacuum concentrator maintaining a temperature of 40 ° C or lower to obtain 12.5 g of dry product.

[Analysis Example 1]

The green tea flower essential oil (essential oil) obtained in Referential Example 1 was poured into a GC injection port and subjected to GC-MS analysis. GC-MS analysis conditions are as follows.

Analysis condition

. Analytical instrument: HP 6890 II GC. Detector: HP 5973 MSD

. Column: PEG-20M (60mX0.25mmX0.25um). Carrier Gas: He

. Injection Temperature: 250 ° C. Detector Temperature: 280 ° C

. Oven Temperature: 70 ° C to 220 ° C (3 ° C / min). Ionization Voltage: 70eV

. Injection Volume: 1ml. Split Ratio: 1:40

As a result, the fragrance components of the green tea essential oil (essential oil) obtained by the steam distillation method are shown in Table 1 below.

Flavor Name content(%) 4,8-Dimethyl-1,3 (E), 7-nonatriene 0.008 Lilac alcohol 0.048 Acetophenone 31.101 Alpha-methylbenzyl alcohol 23.600 2-phenylethyl alcohol 10.150 Linerul 3.530 Zerrani 1.941 Linalool oxide 2.250 Methyl salicylate 0.110 Indole 0.050 vanillin 0.040 Etc 27.172 Total content 100,000

As shown in Table 1, the essential oil of green tea has the unique constituents 4,8-dimetyl-1,3 (E), 7-nonatriene (E-4,8-dimethyl-1,3,7 alpha-methylbenzyl alcohol, 2-phenylethyl alcohol, and the like, which are distinguished from other commonly used essential oils such as rose essential oil and geranium oil, which contain lignocellulose, lauric alcohol, . These major components account for 64.91% of the total.

[Test Example 1] Measurement of inhibitory effect of ultraviolet rays on the production of reactive oxygen species (ROS)

The cell lines used in the experiments were the Dr. Haekaet cell line received from the pre-sale Fusenig (Human kerationocyte HaCaT cell line) by a ball (well) per 2 × 10 ⁴ of a concentration of 96 to 37 ℃ Plate incubator for fluorescence measurement, 5% CO ₂ Lt; / RTI > for 1 day. For comparison of the efficacy with the green tea essential oil obtained from Reference Example 1, the green tea leaf extract of Reference Example 2 and the green tea flower extract of Reference Example 3 were treated for 24 hours as shown in Table 2 below.

100 μl of 20 μM DCFH-DA (2 ', 7-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc.) was added to HCSS (HEPES buffered control salt solution) and incubated at 37 ° C. and 5% CO ₂ for 20 minutes And washed with HCSS. The fluorescence intensity of DCF (dichlorofluorescein) oxidized with ROS at the initial stage after adding 100 μl of HCSS containing the same sample pretreated for 24 hours was measured with a fluorescent plate reader (Ex = 485 nm, Em = 530 nm). The fluorescence intensity of UVB (30 mJ / cm ² ) and immediately after treatment and up to 3 hours of treatment was measured with a fluorescent plate reader (Ex = 485 nm, Em = 530 nm). The results are shown in Table 2 below, in which the untreated control group (ultraviolet control group) inducing ROS production by ultraviolet rays is 100.

Concentration used Rate of ROS generation after 3 hours (%) Ultraviolet control - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 56 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 75 Green tea flower simple extract treated group (Reference Example 3) 0.1 ppm 68

From Table 2, it was confirmed that the green tea essential oil of the present invention effectively reduced the production of reactive oxygen species (ROS) by ultraviolet rays. The green tea oil essential oil of the present invention can effectively protect against oxidative stress and is effective not only in ultraviolet rays but also in harmful environments such as air pollution, It can be understood that the cell can be detoxified from the cell.

[Test Example 2] Measurement of inhibitory effect on biosynthesis of type I collagenase (MMP-1) by ultraviolet light

The human fibroblasts were cultured in a 48-well plate incubator at a concentration of 10 ⁴ , irradiated with UVA A at a rate of 15 J / cm ² after 24 hours, and then added with a green tea leaf simple extract And green tea flower simple extract were replaced with a medium containing the following ingredients as shown in Table 3 below. On the second day of culture, the supernatant was harvested and the amount of type I collagenase produced using the ELISA (AP biotech RPN2610) method was quantitated. The results are shown in Table 3, in which the ultraviolet control group was set at 100 and the comparative values are shown in Table 3.

Concentration used Type I collagenase biosynthesis (%) Ultraviolet control - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 60 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 85 Green tea flower simple extract treated group (Reference Example 3) 0.1 ppm 78

From the above Table 2, it can be seen that the green tea essential oil of the present invention effectively reduces the biosynthesis of type I collagenase by ultraviolet rays, and the effect is greater than that of the green tea leaf extract and the green tea flower extract . Therefore, it can be seen that the expression of collagenase can prevent or suppress the wrinkles generated by decomposition of normally generated collagen in the skin.

[Test Example 3] Measurement of inhibitory effect on alpha biosynthesis of tumor necrosis factor by ultraviolet rays

The human keratinocytes were cultured in a 12-well plate incubator at a concentration of 10 ⁵ , irradiated with ultraviolet ray B at 30 mJ / cm ² after 24 hours, and then treated with green tea leaf simplification The extracts and simple extracts of green tea flowers were replaced with the medium containing as shown in Table 4 below. Cells were harvested 6-24 hours after incubation and the amount of tumor necrosis factor alpha (TNF-a) produced was quantitated using the ELISA (Pharmingen 555212) method. Tumor necrosis factor alpha is an indicator of the microinflammatory response of the skin and is known to mediate the reduction of skin matrix substances and the like. The results are shown in Table 4, in which the ultraviolet control group is set to 100 and the comparative values are shown in Table 4 below.

Concentration used TNF-α biosynthesis (%) Ultraviolet control - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 57 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 75 Green tea flower simple extract treated group (Reference Example 3) 0.1 ppm 78

It can be seen from Table 4 that the green tea essential oil of the present invention effectively reduces the biosynthesis of tumor necrosis factor alpha caused by ultraviolet rays and has a greater effect than the simple green tea leaf extract and green tea flower simple extract Able to know. Therefore, it can be seen that skin micro-inflammation which can be caused by ultraviolet rays, reduction of skin substance due to such skin micro-inflammation, reduction of elasticity, wrinkle formation, etc. can be effectively prevented or improved.

[Test Example 4] Measurement of collagen biosynthesis activity of skin cells

The human fibroblasts were cultured in a 48-well plate incubator at a concentration of 10 ⁴ , and then a green tea leaf simple extract and a green tea flower simple extract were added to a medium containing essential oil as shown in Table 5 for comparison with the essential oil of Essential Example 1 . After 24 hours of incubation, the supernatant was harvested and the amount of procollagen produced was quantitated using the ELISA (Takara MK101) method. The results are shown in Table 5 below.

Concentration used Collagen biosynthesis (%) Untreated control group - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 135 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 98 Green tea flower simple extract treated group (Reference Example 3) 0.1 ppm 104

From Table 5, it was confirmed that the essential oil of green tea of the present invention effectively increased the biosynthesis of type I collagen. Thus, it can be seen that the generation of new collagen is normally promoted to the skin where collagen production decreases with aging, and wrinkles can be prevented or suppressed.

[Test Example 5] Measurement of tropoelastin and fibrinogen production in human skin fibroblasts

The human fibroblasts were cultured in a 48-well plate incubator at a concentration of 10 ⁴ , and then the green tea leaf simple extract and the green tea flower simple extract were added to the medium containing the green tea leaf essential oil (essential oil) . Western blotting was used to quantitate tropoelastin and fibrilin by harvesting the supernatant after 24 hours of incubation. The results are shown in Table 6, where the control group was 100 after image analysis and the comparative values are shown in Table 6.

Concentration used Tropoelastin production (%) Fibril Production (%) Untreated control group - 100 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 125 135 Green tea leaf extract treated group
(Reference Example 2) 0.1 ppm 100 98 Green tea flower simple extract treated group
(Reference Example 3) 0.1 ppm 101 104

From Table 6, it was confirmed that the essential oil of green tea of the present invention effectively increased the biosynthesis of tropoelastin and fibril, two proteins important for skin elasticity. Therefore, it can be seen that the skin elasticity is maintained and the elasticity of the skin elasticity is strengthened by promoting the generation of the new elastic fiber constituting protein normally on the weakened skin according to aging.

[Test Example 6] Differentiation induction test of human keratinocytes

In order to confirm the skin barrier function and the skin moisturizing ability, human keratinocytes cultured in the primary culture by absorbance test were placed in a culture flask and adhered to the bottom. Then, the effect of the green tea flower essential oil (essential oil) of Reference Example 1 , Green tea leaf extract and green tea flower extract were added to the culture solution as shown in Table 7, and the cells were cultured for 5 days until the cells reached 70 ~ 80% of the bottom area. The cells were harvested and washed with PBS (phosphate buffered saline). The cells were washed with 10 mM Tris-HCl buffer (Tris-HCl buffer, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) , pH 7.4), sonication was performed for 3 minutes, and the mixture was boiled for 10 minutes. The precipitate was centrifuged at 1200 rpm for 30 minutes, and the precipitate was suspended in 1 ml of PBS and the absorbance at 340 nm was measured.

Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a criterion when evaluating the degree of cell differentiation. The test results are shown in Table 7 below, in which low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were used as negative / positive control groups and low calcium concentrations were added.

Concentration used The ability to differentiate in keratinocytes (%) Control group Low Ca < ^{2 +} & gt ^; (0.03 mM) 100 Hign Ca < ^{2 +} & gt ^; (1.2 mM) 210 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 124 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 98 Green tea flower simple extract treated group (Reference Example 3) 0.1 ppm 108

As shown in Table 7, the amount of CE (cornified envelope) produced upon keratinocyte differentiation was measured to compare the cell differentiation promoting effect It can be seen that the essential oil of green tea according to the present invention promotes differentiation in keratinocytes. Therefore, it can be seen that the barrier function of the skin is strengthened and the skin moisturizing ability is enhanced.

[Test Example 7] Measurement of filaggrin expression by RT-PCR analysis

The cell lines used in the experiments were the Dr. As the human kerationocyte HaCaT cell line from Fusenig, the cells were divided into 1 × 10 ⁵ cells in a 60 mm dish and cultured for 1 day. Then, the cells were cultured for 1 day in order to compare the efficacy with the green tea essential oil of Reference Example 1 Green tea leaf simple extract and green tea flower simple extract were treated as shown in Table 8 and cultured for 24 hours.

Total RNA was extracted from the cells cultured and tested as described above using triazole (Trizol, Gibco Laboratories, USA), and then the total RNA was extracted by one step RNA PCR kit (AMV) (Takara Bio Inc., Japan) RT-PCR (reverse transcriptional polymerase chain reaction) was performed. Primers for filaggrin (Bioneer, Korea) were used as primers and beta-actin was used as an internal control for comparison. After the image analysis, the control group was set to 100, and the comparative values are shown in Table 8 below.

Concentration used Pillared green production (%) Untreated control group - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 180 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 112 Green tea flower simple extract treated group (Reference Example 3) 0.1 ppm 109

As shown in Table 8, it can be seen that the essential oil of green tea according to the present invention promotes the expression of pilgar green in keratin-forming cells. Therefore, it can promote the production of natural moisturizing factor of the skin and enhance the skin moisturizing ability.

[Test Example 8] Measurement of inhibitory effect on melanin production using mouse pigment cells

Cells were cultured in a 24-well plate incubator at a concentration of 10 < ⁵ > cells of C57BL / 6 mouse-derived rat melanoma cells. Hydroquinone was continuously administered for three consecutive days from the second day, ) As a test substance and cultured. The hydroquinone used herein was a component which inhibits the formation of melanin pigment and has a very strong decolorizing effect. The hydroquinone-treated group was used as a positive control. Then, the culture medium was removed, washed with PBS, and the cells were dissolved with 1 N sodium hydroxide, and the absorbance at 400 nm was measured. The inhibition rate of melanin production was calculated according to the following formula 1, and the results are shown in Table 9 below (Dooley's method).

Concentration used Melanin production inhibition rate (%) Hydroquinone control group 5 [mu] M 56.4 Green tea flower essential oil treatment group 0.1 ppm 34.5 Green tea flower essential oil treatment group 1 ppm 51.6

As shown in Table 9, it was confirmed that the green tea flower essential oil (essential oil) of the present invention exhibited excellent melanin production inhibition rate as compared with hydroquinone. It can be confirmed that it can inhibit pigmentation of skin through this, and it is confirmed that it is effective for skin whitening.

[Test Example 9] Measurement of wrinkle improvement effect

In order to confirm the effect of improving the wrinkles in human by the composition of the present invention, Example 1 was prepared according to the composition ratios shown in Table 10 below, using the external preparation as a nutritional cream. The unit of the content ratio of the following ingredients is% by weight.

Compounding ingredient Production Example 1 Comparative Example 1 Purified water To 100 To 100 Green tea flower essential oil (Reference Example 1) 0.1 - Vegetable hydrogenated oil 1.50 1.50 Stearic acid 0.60 0.60 Glycerol stearate 1.00 1.00 Stearyl alcohol 2.00 2.00 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 Caprylic / carboxy triglyceride 11.00 11.00 Cyclomedicone 6.00 6.00 Preservative, incense Suitable amount Suitable amount Triethanolamine 0.1 0.1

In order to confirm the effect of improving the wrinkles in Comparative Example of Table 10 and Production Example 1, the following evaluation was made. 80 healthy women in 30 groups were divided into two groups of 20 persons for each of the two groups of Comparative Example 1 and Preparation Example 1 to apply the nutritional cream to the face for one week for 8 weeks each day, The state was measured with a visiometer (SV600, Courage + Khazaka electronic GmbH, Germany) and the images were analyzed. The results are shown in Table 11 below. The values in Table 11 below are the average values obtained by subtracting the pre-application parameter values from the respective parameter values after 8 weeks of application.

Clinical results after 8 weeks of use R1 R2 R3 R4 R5 Comparative Example 1 0.27 0.26 0.21 0.03 0.03 Production Example 1 -0.22 -0.21 -0.12 -0.04 -0.03

R1: Difference between the maximum value and the minimum value of the wrinkle contour line

R2: The wrinkle contour line is arbitrarily divided into 5 squares, and the average of R1 values

R3: the highest value of R1 divided by 5

R4: Average value obtained by subtracting the value of each apex and valley from the baseline of the wrinkle contour line

R5: Difference between the baseline of the wrinkle contour line minus the outline of each wrinkle

As shown in Table 11, it was found that the external preparation composition of Preparation Example 1 had a very excellent skin wrinkle-reducing effect. It can be seen that Production Example 1 containing the essential oil of green tea of the present invention has an effect of improving wrinkles as compared with the group of Comparative Example 1 applied.

[Test Example 10] Measurement of skin elasticity improvement effect

In order to confirm the effect of improving the skin elasticity in human by the composition of the present invention, Example 1 of the Test Example 9 and Comparative Example 1 were evaluated as follows.

40 healthy women in 30 groups were divided into two groups of 20 persons for each of the two groups of Comparative Example 1 and Example 1 and the nutritional cream was applied twice daily for 8 weeks at a temperature of 24-26 ° C and a humidity of 75% The skin elasticity was measured using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany). The results are shown in Table 12 below. The results in Table 12 are reported as ΔR8 (R8 (left) -R8 (right)) of Cutometer SEM 575, where the R8 value indicates the nature of the viscoelasticity of the skin.

Clinical results after 8 weeks of use Skin elasticity effect Comparative Example 1 0.10 Production Example 1 0.43

As shown in Table 12, it can be seen that the skin elasticity of Preparation Example 1 containing the essential oil of green tea of the present invention is higher than that of the composition of Comparative Example 1.

At the end of the test, subjects were asked to fill out questionnaires and perform subjective evaluation of efficacy at the same time as the device evaluation. The results are shown in Table 13 below.

Survey results after 8 weeks of use Number of respondents (in) Very good Good usually Inadequate Comparative Example 1 2 5 5 8 Production Example 1 6 9 5 0

As shown in Table 13, the skin elasticity was improved when the composition according to the present invention was applied through the questionnaire.

[Test Example 11] Measurement of skin moisturizing effect

In order to confirm the skin moisturizing effect in humans by the composition of the present invention, Production Example 1 and Comparative Example 1 of Test Example 9 were evaluated as follows.

Forty of 30 healthy women were divided into two groups of 20 persons for each of the two groups of Comparative Example 1 and Example 1, and the nutritional cream was applied twice daily for 4 weeks at a temperature of 24-26 ° C and a humidity of 75% (24 ° C, relative humidity: 40%) after 2 weeks (total 6 weeks) after stopping application and before the start of application and after 1 week, 2 weeks and 4 weeks after application, ) Was used to measure skin moisture content with a coneometer. The results are shown in the following Table 14 as the percentage of increase in the measured value after treatment for a certain period based on the coneometer value measured just before the start of the test.

Clinical outcome Moisture growth rate (%) 1 week Two weeks 4 weeks 6 weeks Comparative Example 1 30 34 34 15 Production Example 1 34 41 42 33

As shown in Table 14, it was confirmed that the skin moisture content of Example 1 containing the essential oil of green tea of the present invention was higher than that of the group to which Comparative Example was applied. In addition, the values after 6 weeks after the measurement of the skin moisture after 2 weeks after stopping the application of the test substance were similar to those after 1 week to 2 weeks after the measurement, and even when the test substance was not applied, The amount of moisture retained by the skin was continuously maintained. Therefore, it can be understood that the essential oil of green tea of the present invention enhances skin moisturization.

Hereinafter, a formulation example of a composition for external application for skin containing essential oil of green tea according to the present invention will be described in more detail with reference to the above test examples, but the present invention is not limited to these formulation examples.

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient weight% Purified water To 100 Green tea flower essential oil (essential oil) 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient weight% Purified water To 100 Green tea flower essential oil (essential oil) 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 3] Nourishing cream

Compounding ingredient weight% Purified water To 100 Green tea flower essential oil (essential oil) 0.1 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 4] Massage cream

Compounding ingredient weight% Purified water To 100 Green tea flower essential oil (essential oil) 0.1 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 5] Pack

Compounding ingredient weight% Purified water To 100 Green tea flower essential oil (essential oil) 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Phage-12 nonylphenyl ether 0.3

Claims (12)

A composition for external application for skin comprising green tea flower essential oil (essential oil) as an active ingredient, which is a green tea flower fragrance ingredient obtained by a steam distillation method after solvent extraction of green tea flowers. delete The method according to claim 1, wherein the green tea flower component is selected from the group consisting of 4,8-dimetyl-1,3 (E), 7-nonatriene (E-4,8-dimethyl- A composition for external application for skin, which comprises an alcohol (lilac alcohol). [4] The composition for external application for skin according to claim 1 or 3, wherein the green tea essential oil is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the composition removes reactive oxygen species (ROS). A composition for external application to skin for preventing skin aging, which contains, as an active ingredient, green tea flower essential oil (essential oil), which is a green tea flower fragrance ingredient obtained through steam distillation after solvent extraction of green tea flowers. A composition for external application for skin for preventing and improving skin inflammation containing green tea flower essential oil (essential oil) as an active ingredient, which is a green tea flower fragrance ingredient obtained through steam distillation after solvent extraction of green tea flowers. A composition for external application for skin for prevention and improvement of skin wrinkle formation, which comprises, as an active ingredient, green tea flower essential oil (essential oil), which is a green tea flower fragrance ingredient obtained through steam distillation after solvent extraction of green tea flowers. A composition for external application for skin for improving skin elasticity comprising, as an active ingredient, green tea flower essential oil (essential oil), which is a green tea flower fragrance ingredient obtained through steam distillation after solvent extraction of green tea flowers. A composition for external skin application for skin whitening comprising, as an active ingredient, green tea flower essential oil (essential oil), which is a green tea flower fragrance ingredient obtained through steam distillation after solvent extraction of green tea flowers. A composition for external application for skin for moisturizing skin, which contains, as an active ingredient, green tea flower essential oil (essential oil), which is a green tea flower fragrance ingredient obtained through steam distillation after solvent extraction of green tea flowers. delete