KR20230130489A - Cosmetic materials for skin-whitening and anti-wrinkle, Cosmetics containing the same, and Manufacturing method thereof - Google Patents

Abstract

The present invention relates to cosmetics using vegetable skin conditioning agents using vegetable extracts, cosmetics using the same, and methods for manufacturing them. More specifically, the present invention relates to cosmetics using vegetable extracts, cosmetics using the same, and methods for producing them. More specifically, the present invention relates to cosmetics that have the effect of suppressing or improving the occurrence of skin wrinkles and have excellent skin improvement effects such as skin whitening. It relates to cosmetics, cosmetics manufactured using them, and methods for manufacturing them.

Description

Skin whitening and skin wrinkle improvement cosmetics, cosmetics containing the same, and manufacturing method thereof {Cosmetic materials for skin-whitening and anti-wrinkle, Cosmetics containing the same, and Manufacturing method thereof}

The present invention relates to a cosmetic that has an effect of suppressing or improving the occurrence of skin wrinkles and has excellent skin improvement effects such as skin whitening, cosmetics manufactured using the same, and a method of manufacturing the same.

The function of cosmetics is not only cleanliness and aesthetic function, but also free radicals caused by external factors such as moisture, fine dust, pollutants, stress, ultraviolet rays, etc., and internal factors such as decreased immune cell function and decreased cell activity. It blocks or suppresses the occurrence of skin aging such as destruction of proteins, nucleic acids, and cell membrane lipids caused by radicals, active oxygen, peroxides, etc.

In addition, cosmetics with whitening, skin wrinkle improvement and suppression, and ultraviolet ray blocking functions are continuously being developed to satisfy consumers' desire for high-functional cosmetics. Among them, products that improve and suppress skin wrinkles are receiving a lot of attention from consumers who want to keep their skin young.

In general, as human skin ages, it loses moisture, loses elasticity, loses color and luster, and becomes rough due to internal or external environmental factors. This is called skin aging, and is a process in which wrinkles appear on the skin and spots, freckles, etc. increase. As skin ages, the thickness of the epidermis, dermis, and subcutaneous tissue layers, which are components of the skin, becomes thinner, and the extracellular matrix (ECM), which gives skin elasticity, changes.

Collagen accounts for approximately 70-80% of the total skin dry weight among the extracellular matrix and is composed of elastin (elastic fiber), proteoglycans, glucosaminoglycan, laminin, and fibro. It maintains elasticity along with nectin (fibronectin), but as you age, the ability to produce collagen decreases rapidly, and as skin cells become oxidized, the skin loses elasticity and excessive wrinkles form, turning it into senile skin.

When you are young, you have a lot of collagen in your body, so your whole body is smooth, but as you get older, collagen is lacking or its quality deteriorates, causing your skin to lose elasticity and wrinkles to form. Wrinkles on the skin are affected by various factors such as skin moisture content, collagen content, and immune function, and it is known that collagen content has the greatest influence.

In vivo, the synthesis and decomposition of extracellular matrix such as collagen is properly regulated, but as aging progresses, the synthesis decreases, and the expression of matrix metalloproteinase (MMP), an enzyme that decomposes collagen, is promoted, thereby promoting skin elasticity. Elasticity decreases and wrinkles form. In addition, the elasticity of the skin decreases due to a decrease in cell activity due to natural aging, internal factors due to the activity of collagenase, and an increase in active oxygen and free radicals due to external factors such as pollution, ultraviolet rays, and stress. do.

There is an urgent need to develop cosmetics that are safe for the skin, easy to use, have high stability and excellent formulation, and have an effect of promoting collagen production.

Korean Patent Publication No. 10-2005-0114269 (December 5, 2005) Korean Patent No. 10-1874467 (2018.06.28.)

The present invention investigated and conducted various physiological activity experiments on natural flora in order to suppress and improve skin aging caused by the external environment and maintain the skin in a healthy state. As a result, among natural plant extracts, Phytoplacenta extract was found to be It has excellent skin wrinkle improvement, skin whitening, and antioxidant effects, and when used together with aloe vera leaf extract, green tea extract, and mixed extracts of pine needles, citron, and artemisia, cola, a proteolytic enzyme that leads to antioxidant properties and skin aging, This invention was completed after it was discovered that the effect of inhibiting the secretion of genase and elastase and promoting collagen biosynthesis was increased. That is, the present invention seeks to provide cosmetics with excellent effects such as skin whitening and skin wrinkle improvement, cosmetics using the same, and methods for manufacturing them.

The present invention to solve the above problems relates to a cosmetic for suppressing and improving skin wrinkles, and includes a vegetable conditioning agent including Phytoplacenta extract, Aloe vera leaf extract, green tea extract, and mixed extract. And, the mixed extract is an extract of a mixture of pine needles, citron, and artemisia.

In addition, the present invention relates to a functional cosmetic with excellent effects of suppressing and improving skin wrinkles, and includes a cosmetic prepared by the method described above, and the cosmetic may be of various formulations such as skin formulation, lotion formulation, emulsion formulation, and serum formulation. You can.

Additionally, the present invention relates to a method for producing the above cosmetics and/or cosmetics.

The cosmetic for suppressing and improving skin wrinkles of the present invention has an antioxidant effect and skin whitening effect that suppresses free radicals generated by the external environment, has excellent moisturizing properties, suppresses and improves the occurrence of skin wrinkles, and slows down skin aging. It has the effect of

Hereinafter, the present invention will be described in more detail.

The cosmetic of the present invention contains purified water, first liquid, second liquid, vegetable conditioning agent, emulsifier, emulsion stabilizer, antioxidant, and chelating agent, preferably 2.0 to 3.0% by weight of the first liquid and 16 to 16% by weight of the second liquid. 20% by weight, 5.0 to 10.0% by weight of vegetable conditioning agent, 0.5 to 1.2% by weight of emulsifier, 0.3 to 1.0% by weight of emulsion stabilizer, 0.001 to 0.050% by weight of antioxidant, 0.01 to 0.05% by weight of chelating agent and the remainder of the total weight%. Contains purified water, more preferably 2.35 to 2.80% by weight of the first liquid, 17.0 to 19.5% by weight of the second liquid, 5.5 to 8.2% by weight of vegetable conditioning agent, 0.5 to 1.0% by weight of emulsifier, and 0.4 to 0.8% by weight of emulsion stabilizer. %, antioxidant 0.002 to 0.040 wt %, chelating agent 0.01 to 0.04 wt %, and the remaining amount of purified water among the total weight %.

Among the cosmetic ingredients, the first and second liquids contain conditioning agents and play a role in providing effects such as skin homeostasis, moisturizing, skin tone improvement, and skin soothing. Together with the vegetable conditioning agent, they have beneficial effects on the skin. To maximize it, it is best to use it within the above content range.

The first liquid is It is prepared by mixing a first conditioning agent with 1,2-hexanediol as a solvent, and the first conditioning agent is RH-oligopeptide-1, adenosine, and SH-polypeptide-1 (SH-Polypeptide-3). and ethylhexylglycerin. And, the amount of these used is 0.2 to 2.0 parts by weight of RH-oligopeptide-1, 1.0 to 3.5 parts by weight of adenosine, and 0.1 to 2.0 parts by weight of SH-polypeptide-1, based on 100 parts by weight of 1,2-hexanediol. and 1.5 to 5.0 parts by weight of ethylhexylglycerin, more preferably 0.3 to 0.8 parts by weight of RH-oligopeptide-1, and 1.2 to 2.8 parts by weight of adenosine, based on 100 parts by weight of 1,2-hexanediol. , 0.2 to 1.5 parts by weight of SH-polypeptide-1 and 1.8 to 3.5 parts by weight of ethylhexylglycerin.

At this time, if the amount of RH-oligopeptide-1 used is less than 0.2 parts by weight, there may be a problem of insufficient skin elasticity and skin texture improvement effects due to its use, and even if used in excess of 2.0 parts by weight, there may be no additional skin improvement effect. Therefore, it is recommended to use it within the above range. In addition, if the amount of adenosine used is less than 1.0 parts by weight, the synergistic effect of improving skin wrinkles due to its use may be minimal, and if it is used in excess of 3.5 parts by weight, there may be a problem of reducing the skin moisturizing properties of the cosmetic. In addition, if the amount of SH-polypeptide-1 used is less than 0.1 parts by weight, there may be no anti-aging effect, and if it is used in excess of 2.0 parts by weight, it may cause skin problems, so it should be within the above range. It's good to use. In addition, if the amount of ethylhexylglycerin used is less than 1.5 parts by weight, the skin moisturizing effect may be minimal, and if it is used in excess of 5.0 parts by weight, the miscibility between the components of the first liquid and the solubility in the solvent decrease, resulting in phase separation. There may be a problem.

Next, the second liquid is prepared by mixing butylene glycol with a second conditioning agent, pH adjuster, and moisturizer, and contains 150 to 200 parts by weight and 10 to 20 parts by weight of pH adjuster based on 100 parts by weight of butylene glycol. and 30 to 50 parts by weight of a humectant, more preferably 165 to 190 parts by weight, 10.5 to 16.5 parts by weight of a pH adjuster, and 37 to 46 parts by weight of a humectant, based on 100 parts by weight of butylene glycol. . At this time, if the second conditioning agent is less than 150 parts by weight, the skin improvement effect may be insufficient and marketability may be lowered, and if it exceeds 200 parts by weight, it may actually cause skin problems, so it is better to use it within the above range. In addition, if the amount of the pH adjuster used is outside the above range, the pH of the second liquid may be too high or too low, which may cause problems with poor compatibility with other cosmetic ingredients. If the amount of the moisturizer used is less than 30 parts by weight, the use of the moisturizer may be harmful. The effect of improving the moisturizing properties of cosmetics may be minimal, and if used in excess of 50 parts by weight, the viscosity of the second liquid may become too high, resulting in poor miscibility with other cosmetics.

The second conditioning agent is arginine, niacinamide, Bis-PEG-18methyl ether dimethyl silane, Glycereth-26, and panthenol. (panthenol), sodium hyaluronate, hydrogenated polydecene, and hydrolyzed collagen. The arginine plays a role in maintaining skin homeostasis through moisturizing and moisture replenishment, and the niacinamide plays a role in imparting a skin whitening effect to cosmetics. In addition, the bis-PEG-18methyletherdimethylsilane plays a role in increasing the miscibility and solubility between the moisturizing and second conditioning agent components, glycereth-26 plays a role in increasing skin moisturization, and panthenol serves as a skin moisturizing ingredient. It helps in absorption. In addition, sodium hyaluronate serves to moisturize the skin, hydrogenated polydecene blocks evaporation of skin moisture at ℃ and improves skin exfoliation, and hydrolyzed collagen serves to increase skin elasticity.

And, the amount of each second conditioning agent used is expressed based on butylene glycol, based on 100 parts by weight of butylene glycol. Arginine 50 to 65 parts by weight, niacinamide 30 to 45 parts by weight, Bis-PEG-18methyletherdimethylsilane 30 to 40 parts by weight, Glycereth-26 20 to 30 parts by weight, panthenol 5 to 15 parts by weight, sodium 0.1 to 2.0 parts by weight of hyaluronate, 3.0 to 10.0 parts by weight of hydrogenated polydecene, and 1.0 to 4.0 parts by weight of hydrolyzed collagen, preferably based on 100 parts by weight of butylene glycol. Arginine 52 to 60 parts by weight, niacinamide 35 to 45 parts by weight, Bis-PEG-18methyletherdimethylsilane 35 to 40 parts by weight, Glycereth-26 22 to 28 parts by weight, panthenol 7 to 13 parts by weight, sodium 0.1 to 1.0 parts by weight of hyaluronate, 4.0 to 9.0 parts by weight of hydrogenated polydecene, and 1.5 to 3.0 parts by weight of hydrolyzed collagen.

Among the second liquid components, the pH adjuster may be a general pH adjuster used in the industry, and citric acid may be preferably used.

also. Among the second liquid components, the moisturizer may be a general moisturizer used as a cosmetic moisturizing component, preferably at least one selected from PEG-32 and glycerin, and more preferably PEG-32 and glycerin. It is recommended to mix and use at a weight ratio of 1:2.00 to 3.00.

Next, the vegetable conditioning agent in the cosmetic composition includes Phytoplacenta extract, Aloe vera leaf extract, green tea extract, and mixed extract.

The Dolkong Phytoplacenta extract includes the first step of cultivating natural sprouted soybeans; and a second step of separating and obtaining Phyto placenta by stripping the embryos (germs) of cultured natural sprouted soybeans; Step 3 of obtaining an extract by performing an extraction process on the separated phytoplacenta with an ethanol aqueous solution at a concentration of 40 to 55% by volume; and 4 steps of filtering and vacuum concentrating the obtained extract.

Placenta means placenta in English, and the placenta is rich in essential nutrients for the maturation of the fetus. Animal placenta has a risk of disease and infection, but plant-based placenta is not only safe but also contains many bioactive substances. In the cosmetic of the present invention, the soybean phytoplacenta is rich in nutrients such as essential amino acids such as leucine, lysine, valine, soleonine, and isoleucine, as well as dozens of amino acids and various enzymes, minerals, active peptides, proteins, and vitamins necessary for cell production. It contains a variety of antioxidants and plays a role in providing antioxidant, anti-wrinkle, skin regeneration, skin elasticity, skin soothing, and skin whitening effects.

In the third step of the phytoplacenta extract manufacturing process, 10 to 40 parts by weight, preferably 20 to 40 parts by weight, more preferably 25 to 35 parts by weight, are mixed with 100 parts by weight of the ethanol aqueous solution, It is better to obtain the extract by stirring slowly at 25 to 45°C for 48 to 96 hours, preferably at 30 to 45°C for 48 to 84 hours, more preferably at 35 to 45°C for 52 to 76 hours. At this time, if the extraction process temperature exceeds 45°C or the concentration of the ethanol aqueous solution is too high, the beneficial components of phytoplacenta may be deformed, so it is recommended to perform the extraction in the above temperature range.

In addition, the filtration can remove foreign substances in the extract using a general extraction filtration method, and the concentration process is preferably performed under 25 to 45°C using a vacuum concentrator.

In addition, the Aloe vera leaf (Aloe Barbadensis Leaf) extract is an extract obtained by performing hot water extraction or alcohol extraction on dried Aloe vera leaves and then performing an ultrasonic extraction process. For example, in a preferred embodiment, a first step of mixing dried aloe vera leaves with an ethanol aqueous solution at a concentration of 50 to 70% by volume and performing a reflux extraction process at 40 to 60° C. for 2 to 6 hours; Step 2: performing ultrasonic extraction of the refluxed extract; It can be used as prepared by performing a process including three steps: filtering the ultrasonic extract and then vacuum concentrating it.

In the present invention, the aloe vera leaf extract has excellent sterilizing power and provides the effect of relieving heat by providing a refreshing sensation to the skin, alleviating moisture loss, improving skin condensation by supplying moisture, supplying skin nutrition, and strengthening the skin barrier by enhancing immunity, It also serves to reduce the cytotoxicity of plant-based conditioning agents. In addition, the amount of aloe vera leaf extract used is 0.02 to 0.12 weight ratio, preferably 0.04 to 0.10 weight ratio, based on 1 weight ratio of the above-mentioned phytoplacenta extract. When used at less than 0.02 weight ratio, problems with cytotoxicity of cosmetics may occur, and 0.12 weight ratio is used. Using it in excess is an excessive use, and there is no additional skin whitening or skin immunity enhancement effect due to its use. Rather, the amount of other extracts used is relatively small, so it is better to use it within the above range.

In addition, the green tea (Camellia Sinensis Leaf) extract is an extract prepared by hot water extraction or ethanol extraction of dried green tea. For example, in a preferred embodiment, a first step of mixing an aqueous ethanol solution with a concentration of 50 to 70% by volume and dried green tea, then performing a reflux extraction process at 40 to 60° C. for 2 to 6 hours to obtain an extract; Step 3: distillation under reduced pressure of the obtained extract; and 4 steps of drying and powdering.

In the present invention, the green tea extract provides an anti-freckle effect by inhibiting melanin production from antioxidant and ultraviolet rays. In addition, the appropriate amount of green tea extract in the vegetable conditioning agent is 0.02 to 0.10, preferably 0.04 to 0.08, based on 1 weight of the Phytoplacenta extract. If the amount of green tea extract is less than 0.02, melanin production is inhibited by its use. It is not effective, and even if used in excess of 0.10 weight ratio, there may be no additional effect of suppressing melanin production, so it is recommended to use within the above range.

In addition, the mixed extract is a mixed extract of pine leaves (Pinus Densiflora Leaf), citrus (Citrus Junos Fruit), and Artemisia Annua. After preparing dried pine leaves, dried citron, and dried Artemisia Annua, they are mixed. Step 1: prepare the mixture by mixing at a weight ratio of 1:0.5 ~ 2:0.5 ~ 2; and a second step of mixing the mixture with an aqueous ethanol solution at a concentration of 50 to 70% by volume and performing a reflux extraction process at 40 to 60° C. for 2 to 6 hours; Step 3: performing ultrasonic extraction of the refluxed extract; It can be used as prepared by performing a process including the fourth step of filtering the ultrasonic extract and then vacuum concentrating it.

In the present invention, the mixed extract is antibacterial, anti-inflammatory, inhibits sebum secretion, has skin improvement effects such as acne, and serves to increase the wrinkle improvement effect of the Phytoplacenta extract.

In addition, the amount of mixed extract used in the vegetable conditioning agent is 0.10 to 0.50 weight ratio, preferably 0.15 to 0.36 weight ratio, based on 1 weight ratio of the Phytoplacenta extract, and if the mixed extract used is less than 0.10 weight ratio, its use increases the antioxidant properties of cosmetics. The effect of inhibiting elastase activity, inhibiting collagenase secretion, and increasing collagen biosynthetic performance may be minimal, and if used in excess of 0.50 weight ratio, it may cause skin cytotoxicity problems and skin troubles, so it is recommended to use within the above range. .

In addition, if the content of the vegetable conditioning agent described above is less than 5.00% by weight of the total weight% of the cosmetic, the skin wrinkle suppression and skin whitening effect may be significantly insufficient, and even if used in excess of 10.00% by weight, the effect of improving skin wrinkles and/or whitening is increased. It is better to use it within the above range as it may no longer exist or may actually cause skin problems.

Next, the emulsifier in the cosmetic composition dissolves the first liquid, the second liquid, and the vegetable conditioning agent well in purified water, and serves to increase miscibility between cosmetic compositions. The emulsifier is glycol stearate SE and hydrogel. It may contain one or more types selected from nate lecithin, preferably glycol stearate SE and hydrogenate lecithin in a weight ratio of 1:0.5 to 3.0, more preferably glycol stearate SE and hydrogenate lecithin. It can be used by mixing at a weight ratio of 1:0.5 to 1.2.

In addition, if the content of the emulsifier is less than 0.5% by weight of the total weight% of the cosmetic, there may be a problem that the cosmetic composition does not dissolve well in purified water, which is a solvent, and using more than 1.2% by weight is excessive use, which can actually cause skin problems. This may cause it, so it is best to use it within the above range.

Next, the emulsion stabilizer in the cosmetic composition prevents sedimentation from occurring when the cosmetic is stored, and may include one or more selected from cetearyl alcohol, PVM/MA copolymer, and stearic acid, Preferably, the emulsion stabilizer can be used by mixing cetearyl alcohol, PVM/MA copolymer, and stearic acid in a weight ratio of 1:0.10 to 0.50:1.00 to 2.00, and more preferably, cetearyl alcohol, PVM/MA copolymer and stearic acid can be mixed and used at a weight ratio of 1:0.20 ~ 0.30: 1.20 ~ 1.80. In addition, if the emulsion stabilizer content is less than 0.3% by weight of the total weight% of the cosmetic, the long-term storage stability of the cosmetic may decrease and sediment may occur. Using more than 1.0% by weight is excessive use and can actually damage the skin due to the excessive emulsion stabilizer. It is recommended to use within the above range as it may cause problems.

Next, antioxidants in cosmetic compositions serve to provide additional antioxidant effects in addition to the antioxidant effects provided to cosmetics by first and second conditioning agents and/or vegetable conditioning agents, and common antioxidants used in the industry can be used. Preferably, ascorbic acid, tocopherol, sodium ascorbyl phosphate, etc. can be used. In addition, if the antioxidant content exceeds 0.050% by weight of the total weight% of the cosmetic, the effect of the skin conditioning agent in the cosmetic may be reduced, so it is better to use it within the above range.

Next, the chelating agent in the cosmetic composition plays a role in maintaining the dispersion stability of the cosmetic, and a general chelating agent used in the industry can be used, preferably disodium EDTA. there is. In addition, if the chelating agent content in the cosmetic is less than 0.01% by weight, the dispersion stability of the cosmetic may be reduced due to its use and the coagulation effect may not be effective. If it is used in excess of 0.05% by weight, skin problems may occur due to unnecessary overuse. Since there may be problems, it is best to use it within the above range.

The cosmetic of the present invention described above includes a first step of preparing a first liquid and a second liquid, respectively; Step 2 of preparing a mixed solution by mixing and stirring the second liquid in the mixed liquid of purified water and the first liquid; and a third step of mixing and stirring a vegetable conditioning agent, an emulsifier, an emulsion stabilizer, an antioxidant, and a chelating agent in the mixed solution of step two.

The first liquid can be prepared by adding 1,2-hexanediol to the mixer, heating to 38 to 45°C, adding the first conditioning agent, and stirring at 38 to 45°C. .

The second liquid is heated to 45 to 55°C after adding butylene glycol to the mixer, then adding the second skin conditioning agent, pH adjuster and moisturizer, and then heating at 45 to 55°C. It can be prepared by performing stirring.

The specific ingredients and appropriate contents of purified water, first liquid, second liquid, vegetable conditioning agent, emulsifier, emulsion stabilizer, antioxidant, and chelating agent used in the production of cosmetics are as described above.

The present invention can produce cosmetics of various formulations such as suspension, emulsion, paste, skin, serum, essence, lotion, and cream using the cosmetics described above.

A method of manufacturing serum-type cosmetics using the cosmetic of the present invention will be described as follows.

After producing a cosmetic by performing the steps 1 to 3 described above, a step 4 of mixing the cosmetic with a viscosity enhancer, tea tree leaf oil, and fragrance can be performed to produce a serum-type functional cosmetic. there is. The appropriate usage amounts of the viscosity enhancer, tea tree leaf oil, and fragrance are shown in Table 11 below, which is an example and does not limit the present invention thereto.

division Usage (parts by weight) cosmetics 100 parts by weight Viscosity enhancer 1.0 to 4.0 parts by weight, preferably 1.5 to 2.5 parts by weight preservative 0.005 to 0.040 parts by weight, preferably 0.005 to 0.025 parts by weight. tea tree leaf oil 0.020 to 0.150 parts by weight, preferably 0.040 to 0.100 parts by weight. Spices 0.005 to 0.100 parts by weight, preferably 0.010 to 0.060 parts by weight.

Preferred examples of the viscosity enhancer include carbomer, glyceryl acrylate/acrylic acid copolymer, sodium polystyrene sulfonate, and xanthan gum, and preferably carbomer and glyceryl acrylate. / It may contain three or more types selected from acrylic acid copolymer, sodium polystyrene sulfonate, and xanthan gum, and more preferably four types of carbomer, glyceryl acrylate/acrylic acid copolymer, sodium polystyrene sulfonate, and xanthan gum. Can be used by mixing.

In addition, the preservative may be a general preservative used in the cosmetics industry, for example, phenoxyethanol may be used.

In addition, tea tree leaf oil acts as an antioxidant as well as moisturizing and scenting.

In addition, the fragrance may be used depending on the main user of the cosmetics to be manufactured, and may preferably include one or more types selected from cornmint oil and limonene.

In addition, various additives other than the ingredients in Table 11 may be used as long as they do not reduce the effectiveness of the cosmetics of the present invention.

The functional cosmetics of the present invention have excellent antioxidant effects, skin whitening effects, proteolytic enzyme elastase inhibitory activity, collagenase inhibitory activity, and collagen biosynthesis enhancement effects, and are excellent in improving and suppressing skin wrinkles. It can inhibit aging.

The present invention will be described in more detail below through production examples. The following production examples are intended to aid understanding of the present invention, and should not be construed as limiting the scope of the present invention by the production examples.

[Manufacturing example]

Example 1: Preparation of vegetable skin conditioning agent

(1) Preparation of D. phytoplacenta extract

Naturally sprouted soybeans were cultured for 52 hours under 25°C conditions. Next, Phyto placenta was isolated by stripping embryos (germs) of cultured natural sprouted soybeans.

Next, 32 parts by weight of the obtained phytoplacenta was added to 100 parts by weight of an ethanol aqueous solution with a concentration of 50% by volume, and then an alcohol extraction process was performed at 42° C. for 64 hours while stirring very slowly.

Next, after completion of extraction, the extract was filtered through an 80 mesh, 1.2㎛ filter, and the filtrate was vacuum concentrated using a vacuum concentrator under conditions of approximately 35°C to obtain a concentrate, which was then dried and ground. A powdered phytoplacenta extract was obtained.

(2) Preparation of aloe vera leaf extract

After mixing 25 parts by weight of dried aloe vera leaves with 100 parts by weight of ethanol aqueous solution with a concentration of 60% by volume, reflux extraction was performed at 50℃ for 4 hours, and then again at 20℃ under the conditions of 50KHz and 300 Watt. Ultrasonic extraction was performed for 4 hours to obtain an extract.

After filtering the extract, the filtrate was concentrated under reduced pressure at 40°C or lower, freeze-dried, and then pulverized to prepare a powdered aloe vera leaf extract.

(3) Preparation of green tea extract

After mixing 50 parts by weight of dried green tea with 100 parts by weight of ethanol aqueous solution with a concentration of 50% by volume, reflux extraction was performed at 50℃ for 2 hours, and then again at 20℃ for 2 hours under the conditions of 50KHz and 300 Watt. Ultrasonic extraction was performed to obtain the extract.

The extract was filtered, the filtrate was distilled under reduced pressure, and the obtained extract was dried and ground to prepare a powdered green tea extract.

(4) Preparation of mixed extract

After preparing dried pine needles, dried citron, and dried artemisia, a mixture was prepared by mixing them in a weight ratio of 1:1:1.

After mixing 40 parts by weight of the above mixture with 100 parts by weight of an aqueous ethanol solution with a concentration of 60% by volume, reflux extraction was performed at 50°C for 4 hours, and then ultrasonicated at 20°C for 4 hours at 50KHz and 300 Watt. Extraction was performed to obtain an extract.

After filtering the extract, the filtrate was concentrated under reduced pressure and freeze-dried at 40°C or lower, then pulverized to prepare a powdered mixed extract.

(5) The plant-based skin conditioning agent was prepared by mixing the previously prepared D. phytoplacenta extract, aloe vera leaf extract, green tea extract, and mixed extract at a weight ratio of 1:0.04:0.06:0.18.

Examples 2 to 3 and Comparative Examples 1 to 4

A vegetable skin conditioning agent was prepared in the same manner as in Example 1, but each vegetable skin conditioning agent was prepared with the composition and ratio shown in Table 1 below, and Examples 2 to 3 and Comparative Examples 1 to 4 were performed, respectively.

division
(weight ratio) Example 1 Example 2 Example 3 Comparative Example 1 Comparative example 2 Comparative example 3 Comparative example 4 Soybean Phytoplacenta Extract One One One - One One One Aloe Vera Leaf Extract 0.04 0.09 0.09 One 0.04 0.04 - green tea extract 0.06 0.04 0.04 1.5 0.06 0.06 0.06 mixed extract 0.18 0.36 0.10 4.0 0.05 0.65 0.18

Experimental Example 1: MTT cytotoxicity evaluation

Cytotoxicity evaluation was performed using Mosman's method of measuring cell viability using MTT [3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide] reagent.

HDF (Human Derman Fibroblast) was dispensed into a 96-well plate at a concentration of 1×10 ⁴ cells/well and cultured for 24 hours at 37°C in a 5 vol% CO ₂ atmosphere. After re-incubating the sample for 24 hours, the medium was removed and washed twice with PBS (Phosphate buffered saline), and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromid) was added for 5 days. Dissolve in PBS at mg/mL, add 50 μL, and culture for 2 hours at 37°C, 5 vol% CO ₂ .

Afterwards, 100 μL of DMSO (Dimethyl sulfoxide) was added to each well, Examples 1 to 3 and Comparative Examples 1 to 2 were added at different concentrations, left for 20 minutes, and the absorbance was measured at 570 nm. The presence or absence of cytotoxicity was evaluated for each sample concentration, and the results are shown in Table 2 below. The presence or absence of cytotoxicity was evaluated by cell viability based on the control group (see Equation 1).

[Equation 1]

Cell viability (%) = {(Absorbance of extract-treated sample - Absorbance of untreated sample)/Absorbance of untreated sample}

division
(weight ratio) Example 1 Example 2 Example 3 Comparative Example 1 Comparative example 2 Comparative Example 3 Comparative example 4 100㎍/㎖ 100.3 100.1 99.8 100.5 101.3 101.9 100.2 300㎍/㎖ 100.8 100.7 100.2 100.5 100.7 98.7 99.8 600㎍/㎖ 99.9 100.3 99.8 101.5 100.1 91.8 93.1 1,000㎍/㎖ 99.2 99.7 98.6 99.8 99.4 79.4 83.3

As a result of cytotoxicity measurement, it was confirmed that Examples 1 to 3 and Comparative Examples 1 to 2 showed almost no cytotoxicity up to 1000 μg/ml, and could be stably used on the skin. However, in the case of Comparative Example 3, where the mixed extract of pine needles, citron, and artemisia was used in a weight ratio exceeding 0.50, there was a cytotoxicity problem in which the cell viability was greatly reduced starting from 600 ㎍/㎖.

In addition, Comparative Example 4, which did not use aloe vera leaf extract, also showed cytotoxicity problems as the concentration increased, like Comparative Example 3.

Experimental Example 2: Antioxidant efficacy activity evaluation

In the MTT cytotoxicity evaluation performed in Experimental Example 1, the antioxidant efficacy activity of the vegetable skin conditioning agents of Examples 1 to 3 and Comparative Examples 1 to 2, which had no cytotoxicity problems, was evaluated.

To measure the antioxidant effect, L-ascorbic acid was used as a control under laboratory conditions, and the antioxidant activity was measured using the DPPH method.

The DPPH method measured the degree of antioxidant activity by reducing power using a free radical called DPPH (2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl) free radical. The degree to which DPPH was reduced by the test substance and the absorbance decreased was compared with the absorbance of the blank test solution, and the free radical scavenging rate was measured at a wavelength of 515 nm.

The reagent used was a 0.2 mM solution of DPPH free radical (Sigma Aldrich, MW=618.76) dissolved in methanol to make 100 ml.

In the measurement method, 0.15 ml of 0.2 mM DPPH solution and 0.15 ml of sample solution were added to a 96-well plate, quickly stirred, and aging was started at 25°C for 10 minutes. Afterwards, the absorbance at 515 nm was measured using a microplate reader (BioTek, USA), and the DPPH radical removal rate (%) was calculated using Equation 2 below. At this time, 1,000 μg/ml of ascorbic acid was used as a positive control to remove radicals, and 1,000 μg/ml of vegetable skin conditioning agent was also used.

[Equation 2]

DPPH radical scavenging rate (%) = (A - B)/A×100%

In Equation 2 above, A is the absorbance obtained from the blank test solution, and B is the absorbance obtained from the test solution.

The DPPH radical removal rate (%) measurement results are shown in Table 3 below.

division DPPH radical removal rate (%) positive control
(Ascorbic acid 1,000 ㎍/ml) 96.2 Example 1 83.1 Example 2 85.4 Example 3 80.2 Comparative Example 1 57.9 Comparative Example 2 75.6

Looking at the DPPH radical removal rate measurement results in Table 3, it was confirmed that Examples 1 to 3 showed a high DPPH radical removal effect of more than 80%, although it was lower than that of the control group, ascorbic acid.

On the other hand, in the case of Comparative Example 1 in which the DPPH radical removal effect was not used, the DPPH radical removal effect was significantly lower, and it was confirmed that the DPPH radical extract was significantly contributed to the antioxidant effect.

In addition, in the case of Comparative Example 2, in which the mixed extract was used at a weight ratio of less than 0.10, compared to Example 3, the antioxidant enhancement effect due to use together with the Rhododendron phytoplacenta extract was significantly reduced.

Experimental Example 3: Evaluation of efficacy of inhibiting elastase activity

An evaluation of the elastase activity inhibitory effect of the vegetable skin conditioning agents of Examples 1 to 3 and Comparative Examples 1 to 2 was performed.

Elastase is an enzyme that decomposes elastin, an important substrate for maintaining skin elasticity in the dermis. Therefore, skin aging can be suppressed by reducing the activity of elastase, which is one of the main causes of skin aging.

Using N-succinyl-(L-Ala)3-p-nitroanilide (S4760 from Sigma) as a substrate, the amount of p-nitroanilide produced from the substrate was measured at 445 nm for 20 minutes at 37°C. Prepare each test solution to a certain concentration, take 0.5 mL each into a test tube, and add 0.5 mL of porcine pancreatic elastase (Sigma Co, 2.5 U/mL) solution dissolved in 50 mM tris-HCl buffer (pH 8.6). As a substrate, N-succinyl-(L-Ala)3-p-nitroanilide (0.5 mg/mL) dissolved in 50 mM tris-HCl buffer (pH 8.6) was added and reacted for 20 minutes to measure the results. It is shown in Table 4. Oleonolic acid was used as a positive control, the amount of oleonolic acid injected was 0.05% by weight, and the amount of vegetable skin conditioning agent injected was 1,000 ㎍/ml.

division Elastase activity inhibition rate (%) Positive control group
(oleonolic acid 0.05%) 74.1 Example 1 61.5 Example 2 65.2 Example 3 59.7 Comparative Example 1 34.8 Comparative Example 2 54.2

Looking at the elastase activity inhibition evaluation results in Table 4 above, it was confirmed that Examples 1 to 3 and Comparative Example 2 using the Phytoplacenta extract of D. soybean had a significantly higher elastase activity inhibition effect than Comparative Example 1.

In addition, in the case of Comparative Example 2, in which the mixed extract in the vegetable skin conditioning agent was used at a weight ratio of less than 0.10, compared to Example 3, the effect of inhibiting elastase activity due to use together with the Soybean phytoplacenta extract was significantly reduced.

Experimental Example 4: Evaluation of collagenase secretion inhibition efficacy

The collagenase secretion inhibition efficacy of the vegetable skin conditioning agents of Examples 1 to 3 and Comparative Examples 1 to 2 was evaluated at each concentration (300ppm, 1000ppm).

Collagenase is an enzyme that decomposes collagen, an important substrate for suppressing skin wrinkles and maintaining elasticity in the dermis. Therefore, secretion of collagenase accelerates skin aging and causes wrinkles.

To evaluate collagenase secretion (activity) inhibition, 4-phenylazobenzyloxycarbonyl-Pro-LeuGly-Pro-D-Arg (0.3mg/mL) was added to the 0.1 M tris-HCl buffer (pH 7.5) reaction zone by adding 4 nM CaCl ₂ . Add 0.15 mL of collagenase (0.2 mg/mL, Sigma Co) to a mixture of 0.25 mL of dissolved substrate solution and 0.1 mL of sample solution, leave it at room temperature for 20 minutes, and add citric acid at a concentration of 6% by weight. After stopping the reaction by adding 0.5 mL, 1.5 mL of ethyl acetate was added and the absorbance was measured at 320 nm. The results are shown in Table 5 below.

Collagenase secretion inhibition rate (%) 300 ppm 1000ppm Positive control group (ascorbic acid) 60.9 73.2 Example 1 44.7 62.1 Example 2 45.3 65.4 Example 3 39.8 59.3 Comparative Example 1 16.5 38.9 Comparative Example 2 28.7 51.0

Looking at the results of the evaluation of collagenase secretion (activity) inhibition in Table 5 above, it can be seen that Examples 1 to 3 and Comparative Example 2 using the Phytoplacenta extract of D. soybean have a significantly higher collagenase secretion inhibition effect than Comparative Example 1. This trend was the same as Experimental Example 3.

In addition, in the case of Comparative Example 2, in which the mixed extract in the vegetable skin conditioning agent was used at a weight ratio of less than 0.10, the effect of inhibiting collagenase secretion was significantly reduced compared to Examples 1 to 3.

Comparative Example 5

A vegetable skin conditioning agent was prepared in the same manner as in Example 1, except that when preparing the mixed extract, mugwort was not used, and only dried pine needles and dried citron were mixed in a 1:1 weight ratio to prepare a mixed extract. A vegetable skin conditioning agent was prepared by mixing phytoplacenta extract, aloe vera leaf extract, green tea extract, and the above mixed extract in a weight ratio of 1:0.04:0.06:0.18.

Experimental Example 5: Evaluation of collagen biosynthetic performance (Collagen Contents)

Collagen biosynthetic performance was measured at each concentration (500 ppm, 1000 ppm) for each of the vegetable skin conditioning agents in Examples 1 to 3 and Comparative Examples 1 to 5, and the results are shown in Table 6 below. The evaluation method is as follows. Same as

HDF (Human Dermal Fibroblast, ccd 986sk) cells were distributed at 1×10 ⁵ cell/well in a 24-well plate and then cultured in DMEM (DMEM) containing 10% FBS, penicillin (50U/ml), and streptomycin (50/ml). Dulbecco's Modified Eagle's Medium, Gibco, USA) was cultured in a 37°C, 5% CO ₂ incubator for 24 hours. After removing the medium and washing with PBS, heat stimulation was applied at 12.5 mJ at a UV wavelength of 312 nm, and then each extract was treated using serum-free medium, and further cultured for 48 to 72 hours. . Afterwards, the amount of collagen synthesis was measured in the cell culture medium by enzyme-linked immunosorbent assay (ELISA) using the Pro-collagen Type I C-peptide EIA Kit (Takara, Japan).

200 ppm of Oleanolic acid was used as a positive control, and 500 ppm and 1000 ppm were used in Examples 1 to 3, respectively. The collagen synthesis rate was expressed as a relative collagen synthesis rate based on the amount of collagen synthesis in the positive control group. did.

Collagen synthesis rate (%) 500ppm 1000ppm Positive control group (oleonolic acid) 100% 100% Example 1 87.7% 98.5% Example 2 88.9% 102.7% Example 3 85.2% 93.7% Comparative Example 5 68.8% 87.4%

Through the experimental results in Table 6, it was confirmed that the vegetable skin conditioning agents of Examples 1 to 3 showed an increase in the collagen synthesis rate in a concentration-dependent manner, and it was confirmed that the complex extract fermentation product fermented thereof significantly increased collagen biosynthetic performance. .

However, Comparative Example 5, a vegetable skin conditioning agent prepared using mixed extracts without Artemisia, showed low collagen biosynthetic performance compared to Examples 1 to 3.

Preparation Example 1: Preparation of skin whitening and skin wrinkle improvement cosmetics and serum formulation cosmetics

(1) Preparation of first liquid

After adding 1,2-hexanediol to the mixer, heating until it reaches 40°C, then adding RH-oligopeptide-1, adenosine, SH-polypeptide-1, and ethylhexylglycerin in order. , The first liquid was prepared by slowly stirring at 40°C at a speed of about 200 rpm. At this time, the content of each composition in the first liquid composition is shown in Table 6 below.

(2) Preparation of second liquid

After adding butylene glycol to the mixer, heating it to 50℃, then adding the second skin conditioning agent arginine, niacinamide, bis-PEG-18methyletherdimethylsilane, glycereth-26, panthenol, Sodium hyaluronate, hydrogenated polydecene, and hydrolyzed collagen were added sequentially. Next, the pH adjuster citric acid and moisturizer (glycerin and PEG-32 mixed at a weight ratio of 1:2.29) were added here, and then stirred at a speed of about 300 rpm at 50°C to prepare the second liquid. was manufactured. At this time, the content of each composition in the second liquid composition is shown in Table 7 below.

(3) Manufacturing of cosmetics and serum-type cosmetics

Purified water was added to the reactor, and then the second liquid was added to the mixer containing the previously prepared first liquid, and then stirred for 10 minutes at about 27°C at a speed of about 300 rpm to prepare a mixed solution.

Next, the vegetable conditioning agent prepared in Example 1, glycol stearate SE (emulsifier), hydrogenate lecithin (emulsifier), cetearyl alcohol (emulsification stabilizer), and PVM/MA copolymer were added to the mixed solution. (emulsion stabilizer), stearic acid (emulsion stabilizer), sodium ascorbyl phosphate (antioxidant), and disodium EDTA (chelating agent) were added, then stirred for 60 minutes at about 27°C at a speed of about 300 rpm to prepare cosmetics. was manufactured.

As an emulsifier, glycol stearate SE and hydrogenate lecithin were used in a weight ratio of 1:1, and as an emulsification stabilizer, cetearyl alcohol, PVM/MA copolymer, and stearic acid were used in a weight ratio of 1:0.24:1.50. did.

Next, in the reactor where the cosmetic was manufactured, based on 100 parts by weight of the cosmetic, 0.2036 part by weight of carbomer (viscosity enhancer), 0.5091 part by weight of glyceryl acrylate/acrylic acid copolymer (viscosity enhancer), and sodium polystyrene sulfonate (viscosity enhancer) were added to the reactor. Enhancer) 0.3054 parts by weight, xanthan gum (viscosity enhancer) 0.7127 parts by weight, phenoxyethanol (preservative) 0.0102 parts by weight, tea tree leaf oil (antioxidant and moisturizing) 0.0611 parts by weight, cornmint oil (fragrance) 0.0402 parts by weight, and limonene (Fragrance) 0.0007 parts by weight was added and stirred to prepare serum-type cosmetics. The amounts used are shown in Table 8 below and expressed as parts by weight for 100 parts by weight of cosmetics.

Comparative Manufacturing Example 1

Cosmetics and serum-type cosmetics were prepared using the same method, composition, and content as Example 1 and Preparation Example 1, but no vegetable conditioning agent was used.

division 1st liquid
(part by weight) 2nd liquid
(part by weight) 1,2-hexanediol 100 - RH-Oligopeptide-1 0.4 - Adenosine 1.6 - SH-polypeptide-1 0.4 - Ethylhexylglycerin 2 - Butylene glycol - 100 arginine - 55.6 Niacinamide - 37.0 Bis-PG-18methyletherdimethylsilane - 37.0 Glycereth-26 - 27.8 panthenol - 11.1 Sodium Hyaluronate - 0.7 Hydrogenated polydecene - 5.6 Hydrolyzed Collagen - 1.9 Citric Acid (pH adjuster) - 13.0 PEG-32, glycerin (moisturizer) - 42.6

division Cosmetics (% by weight) Serum formulation
cosmetics Manufacturing Example 1 Comparative Manufacturing Example 1 Based on 100 parts by weight of cosmetics Purified water 71.57 74.09 - 1st liquid 2.65 3.52 2nd liquid 18.07 21.00 vegetable conditioning agent Example 1 6.52 - emulsifier 0.60 0.71 Emulsion stabilizer 0.56 0.65 antioxidant Sodium Ascorbyl Phosphate 0.01 0.01 Chelating agent Disodium EDTA 0.02 0.02 Total cosmetics 100% by weight 100% by weight Viscosity enhancer
(Carbomer, glyceryl acrylate/acrylic acid copolymer, sodium polystyrene sulfonate, xanthan gum) 1.7308 parts by weight Preservative (phenoxyethanol) 0.0102 parts by weight tea tree leaf oil 0.0611 parts by weight Fragrance (corn mint oil, limonene) 0.0409 parts by weight

Experimental Example 6: Evaluation of tyrosinase enzyme activity inhibition ability

The whitening activity effect of the cosmetic of Preparation Example 1 was evaluated through the ability to inhibit tyrosinase enzyme activity. Here, cosmetics refer to cosmetics that do not contain viscosity enhancers, preservatives, tea tree leaf oil, or fragrance.

Mix 1 mL of 0.1M sodium phosphate buffer (pH 6.9), 1 chemical sample prepared at various concentrations, and 30 ㎕ of mushroom tyrosinase (1 KU/mL), react at 37°C for 10 minutes, and add 1.5mM L-tyrosine 30 as a substrate. ㎕ was mixed and reacted at 25°C for 2 minutes. DOPA chrome produced in the reaction solution was measured at 475 nm, and the results are shown in Table 9. The absorbance of the control group was referred to as Ab _control and the absorbance of the sample was taken as Ab _sample , and was calculated according to Equation 3 below.

[Equation 3]

Tyrosinase enzyme activity inhibition rate (%) = (1-Ab _sample )/Ab _control ×100%

division
(Cosmetic sample concentration, sample) Tyrosinase enzyme activity inhibition rate (%) 0.1mg/ml 8.5% 0.3mg/ml 17.5% 0.5mg/ml 21.3% 0.7mg/ml 28.5% 1.0mg/ml 33.8% 1.5mg/ml 36.1%

Through the evaluation of the tyrosinase enzyme activity inhibition rate in Table 9 above, it was confirmed that the cosmetic of the present invention has a skin whitening effect through inhibiting the activity of the tyrosinase enzyme.

Experimental Example 7: Measurement of skin elasticity improvement effect

The effect of improving skin elasticity before and after use of the serum formulation cosmetics of Preparation Example 1 and Comparative Preparation Example 1 was measured. The test area was measured three times using a Cutometer (Courage and Khazaka Electronic, Germany), and the average value was calculated using the three values. Saved.

The measurement area was measured 3cm away from the corner of the eye. Cutometer applies negative pressure to the skin surface and measures the degree to which the skin is sucked into the probe using an optical system that can pass through the skin. The light used at this time is infrared, and the degree of elasticity is measured by the ratio of the intensity of light that decreases as it passes through the skin and the intensity of light that does not pass through the skin. R2 (A.U.) was selected as the Cutometer measurement variable for elasticity.

The variable R2 represents the ratio of the total contracted length of the skin to the maximum that the skin can stretch, and represents the overall elasticity, which is the re-deformability of the skin. The closer the value of this variable is to 1, the more elastic it is. The skin elasticity improvement rate was calculated according to Equation 3 below. Measurements were taken before use (0 weeks), after 2 weeks of use, and after 4 weeks of use.

No skin adverse events occurred due to the test product during the evaluation period, and the skin elasticity measurement results are shown in Table 10 below.

[Equation 4]

Skin elasticity improvement rate (%) = {(A-B)/B}×100%

In equation 4, A is the R2 measurement value after using the product, and B is the R2 measurement value before using the product.

division 0 weeks
R2 measure 2 weeks later
R2 measure 4 weeks later
R2 measure 4 weeks later
skin elasticity
Improvement rate (%) Comparative Manufacturing Example 1 0.6928 0.6993 0.7002 1.0681 Manufacturing Example 1 0.7012 0.7125 0.7232 2.0679

Looking at the results of measuring the skin elasticity improvement rate, the cosmetics of Preparation Example 1 using a vegetable skin conditioning agent showed a relatively very high skin elasticity improvement effect compared to Comparative Preparation Example 1 which did not use the vegetable skin conditioning agent.

Claims (10)

Contains purified water, first liquid, second liquid, vegetable conditioning agent, emulsifier, emulsion stabilizer, antioxidant, and chelating agent,
The vegetable conditioning agent includes phytoplacenta extract, aloe vera leaf extract, green tea extract, and mixed extract,
A skin whitening and skin wrinkle improvement cosmetic, characterized in that the mixed extract is an extract of a mixture of pine needles, citron, and artemisia.
The method of claim 1, wherein the first liquid includes a first skin conditioning agent and 1,2-hexanediol,
The first skin conditioning agent is a skin whitening and skin wrinkle improvement cosmetic comprising RH-oligopeptide-1, adenosine, SH-polypeptide-1, and ethylhexylglycerin.
The method of claim 1, wherein the second liquid includes a second skin conditioning agent, a pH adjuster, a moisturizer, and butylene glycol,
The second skin conditioning agent includes arginine, niacinamide, bis-PEG-18methyletherdimethylsilane, glycereth-26, panthenol, sodium hyaluronate, hydrogenated polydecene, and hydrolyzed collagen. And
A skin whitening and skin wrinkle improvement cosmetic, characterized in that the moisturizer includes glycerin and PEG-32.
The skin whitening method according to claim 1, wherein the vegetable conditioning agent includes phytoplacenta extract, aloe vera leaf extract, green tea extract, and mixed extract in a weight ratio of 1:0.02 to 0.12:0.02 to 0.10:0.10 to 0.50. and skin wrinkle improvement cosmetics.
The method of claim 1, wherein the emulsifier includes glycol stearate SE and hydrogenate lecithin,
A skin whitening and skin wrinkle improvement cosmetic, characterized in that the emulsion stabilizer includes cetearyl alcohol, PVM/MA copolymer, and stearic acid.
A skin whitening and skin wrinkle improvement cosmetic comprising the cosmetic of any one of claims 1 to 5.
The method of claim 6, wherein the cosmetic is a serum formulation cosmetic,
Skin whitening and skin wrinkle prevention, comprising 1.5 to 2.5 parts by weight of a viscosity enhancer, 0.005 to 0.025 parts by weight of a preservative, 0.040 to 0.100 parts by weight of tea tree leaf oil, and 0.010 to 0.060 parts by weight of fragrance, based on 100 parts by weight of the cosmetic. Improved cosmetics.
The skin whitening and skin wrinkle improvement cosmetic according to claim 7, wherein the viscosity enhancing agent includes one or more selected from carbomer, glyceryl acrylate/acrylic acid copolymer, sodium polystyrene sulfonate, and xanthan gum.
Step 1 of preparing the first and second liquids, respectively;
Step 2 of preparing a mixed solution by mixing and stirring the second liquid in the mixed liquid of purified water and the first liquid; and
The process includes a third step of mixing and stirring the vegetable conditioning agent, emulsifier, emulsion stabilizer, antioxidant, and chelating agent in the mixed solution of step two,
The first liquid is prepared by adding 1,2-hexanediol to the mixer, heating to 38 to 45°C, adding the first conditioning agent, and stirring at 38 to 45°C,
The second liquid is prepared by adding butylene glycol to the mixer, heating it to 45 to 55°C, adding the second skin conditioning agent, pH adjuster and moisturizer, and stirring at 45 to 55°C. It was done,
The first conditioning agent includes RH-oligopeptide-1, adenosine, SH-polypeptide-1, and ethylhexylglycerin,
The second conditioning agent includes arginine, niacinamide, bis-PEG-18methyletherdimethylsilane, glycereth-26, panthenol, sodium hyaluronate, hydrogenated polydecene, and hydrolyzed collagen. A method of manufacturing a skin whitening and skin wrinkle improvement cosmetic, characterized in that.
As a method for manufacturing serum formulation cosmetics,
Step 1 of preparing the first and second liquids, respectively;
Step 2 of preparing a mixed solution by mixing and stirring the second liquid in the mixed liquid of purified water and the first liquid;
Step 3 of producing cosmetics by mixing and stirring vegetable conditioning agents, emulsifiers, emulsion stabilizers, antioxidants, and chelating agents in the mixture of step 2; and
Performing a process including 4 steps of mixing a viscosity enhancer, tea tree leaf oil, and fragrance with the cosmetic,
The first liquid is prepared by adding 1,2-hexanediol to the mixer, heating to 38 to 45°C, adding the first conditioning agent, and stirring at 38 to 45°C,
The second liquid is prepared by adding butylene glycol to the mixer, heating it to 45 to 55°C, adding the second skin conditioning agent, pH adjuster and moisturizer, and stirring at 45 to 55°C. It was done,
The first conditioning agent includes RH-oligopeptide-1, adenosine, SH-polypeptide-1, and ethylhexylglycerin,
The second conditioning agent includes arginine, niacinamide, bis-PEG-18methyletherdimethylsilane, glycereth-26, panthenol, sodium hyaluronate, hydrogenated polydecene, and hydrolyzed collagen. A method for manufacturing skin whitening and skin wrinkle improvement cosmetics.