KR102499608B1 - Rejuvenating serum type cosmetic composition and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a rejuvenating serum-type cosmetic composition and a preparation method thereof and, more specifically, to a serum-type cosmetic composition and a preparation method thereof, which not only exhibits excellent antioxidant and anti-inflammatory activities, but also has skin moisturizing, skin regeneration, and skin irritation relieving effects, thereby showing rejuvenating efficacy. The serum-type cosmetic composition of the present invention comprises: a solvent including purified water, 1,2-hexanediol, diethoxyethyl succinate, and t-butyl alcohol; polyols including caprylyl glycol, butylene glycol, dipropylene glycol, and glycerin; allantoin; arginine; polydecene; calcium carbonate; an extract containing one or more selected from the group consisting of a Prunella extract, an edelweiss callus culture extract, a star anise extract, a hydrolyzed goldenrod nest extract, a bladderwrack extract, and combinations thereof; an emulsifier; and non-polar moisturizing oil.

Description

Rejuvenating serum-type cosmetic composition and manufacturing method thereof

The present invention relates to a rejuvenating serum-type cosmetic composition and a method for producing the same, and more particularly, to a rejuvenating serum-type cosmetic composition that exhibits excellent antioxidant and anti-inflammatory activities as well as skin moisturizing, skin regeneration, and skin irritation relief effects. It relates to a serum-type cosmetic composition capable of exhibiting a ting effect and a method for preparing the cosmetic composition.

As the average lifespan has been extended due to the development of medical technology, the desire to maintain youth as long as possible has become a common concern for men and women of all ages, and the desire to prevent skin aging, which is most easily revealed, is increasing. Accordingly, research on the development of materials capable of finding the cause of skin aging and suppressing it is being actively conducted. Skin aging is divided into three main categories depending on the cause.

The first is natural aging (intrinsic aging). As the body ages, the body's immune system and physiological activity decline, preventing smooth recovery of skin components such as hyaluronic acid and collagen, resulting in reduced skin elasticity and dryness, resulting in fine wrinkles, etc. is formed Hyaluronic acid is one of the components of the extracellular matrix and accounts for only 0.1 to 0.2% in terms of dry weight, but forms a network with other components of the dermis to connect cells to each other, deliver nutrients, and prevent evaporation of moisture from the skin. At the same time, strong bonding with water molecules enables the skin to maintain a certain amount of moisture. The decrease of hyaluronic acid in the skin starts from the age of 18 to 20, and decomposition is accelerated by aging or environmental pollution or exposure to ultraviolet rays. may become For this reason, approaches have been made to prevent aging by supplementing hyaluronic acid in the skin, which is reduced with age. In addition, since collagen accounts for 70 to 80% of the entire dermis, degradation of collagen is closely related to reduction of skin elasticity and formation of wrinkles.

Second, extrinsic aging is aging caused by external stress such as ultraviolet rays. Ultraviolet rays synthesize vitamin D in the body and have a role in sterilization, but also cause dermatitis, skin cancer, sunburn, skin aging, dryness, and fine wrinkles. Ultraviolet (UV) is divided into three types, A, B, and C, depending on the wavelength. Among them, UVC is blocked by the ozone layer, and UVA and UVB affect the skin.

In particular, medium-wavelength UVB (290-320 nm) causes serious skin damage when acutely or chronically exposed. Collagen, elastin, hyaluronic acid, etc. causes loss of skin elasticity, resulting in thick wrinkles. In addition, active oxygen generated by ultraviolet rays damages the intracellular signal transduction system and destroys collagen by increasing the expression of matrix metalloproteinase-1 (MMP-1), a collagen degrading enzyme in the skin.

Third, active oxygen that causes damage to skin cells can be mentioned. When excessively active oxygen is present, it breaks down the body's antioxidant defense system and damages cellular components such as proteins, lipids, and DNA. As a result, cell functions are altered and ultimately skin aging can be accelerated. In addition, active oxygen not only directly destroys collagen by acting on collagen metabolism, but also damages connective tissue by causing collagen deficiency. In addition, active oxygen has the ability to contain a large amount of water in the space between cells, maintaining skin elasticity and cutting the hyaluronic acid chain, which plays a role in absorbing shock, reducing skin elasticity and skin moisture. and thereby accelerate skin aging.

These active oxygen are generated not only from various factors that threaten our health, such as smoking, stress, excessive exercise, skin disease, viral infection, and ultraviolet rays, but also during the process of breathing and digestion, which are essential for maintaining life.

Therefore, it is required to develop a new cosmetic composition to protect the skin from the aging factors and improve damaged skin to maintain clear and elastic skin.

Republic of Korea Patent Publication No. 10-2013-0015339

The present invention is to solve the above problems, and provides a serum-type cosmetic composition capable of imparting excellent anti-inflammatory and antioxidant effects to the skin and exhibiting a rejuvenating effect through skin moisturizing, skin regeneration, and skin elasticity improvement want to do

The above and other objects and advantages of the present invention will become apparent from the following description of preferred embodiments.

The above object is a solvent containing purified water and 1,2-hexanediol; polyols including caprylyl glycol and glycerin; Diisostearyl maleate, silica silylate, cetylethylhexanoe The above object is a solvent containing purified water, 1,2-hexanediol, diethoxyethyl succinate, and t-butyl alcohol; polyols including caprylyl glycol, butylene glycol, dipropylene glycol, and glycerin; allantoin; arginine; polydecene; calcium carbonate; An extract containing at least one selected from the group consisting of Lamiaceae extract, edelweiss callus culture extract, star anise extract, hydrolyzed golden snake nest extract, bladderwrack extract, and combinations thereof; emulsifier; It can be achieved by a serum-type cosmetic composition comprising; and non-polar moisturizing oil.

Specifically, the emulsifier is lysolecithin, glyceryl stearate, polyglyceryl-2 stearate, hydrogenated grape seed alcohol, stearic acid, stearyl alcohol, caprylyl/capryl glucoside, hydrogenated lecithin , hydrolyzed jojoba esters, glyceryl caprylates, alkylamidoalkylamines, and combinations thereof.

Specifically, the non-polar moisturizing oil contains at least one selected from the group consisting of hydrogenated olefin polymer, squalane, hydrogenated polydecene, caprylic/capric triglyceride, dicaprylyl ether, and combinations thereof. It can be characterized by including.

Preferably, the cosmetic composition further comprises one or more additives selected from the group consisting of preservatives, skin conditioning agents, thickeners, antioxidants, chelating agents, defoaming agents, pigments, and combinations thereof. can do.

Specifically, the skin conditioning agent is selected from the group consisting of sodium DNA, sodium hyaluronate, ethylhexyl glycerin, copper tripeptide-1, glycereth-25 PCA isostearate, adenosine, and combinations thereof It may be characterized by including one or more that are.

Specifically, the thickener is acrylate/C10-30 alkyl acrylate crosspolymer, xanthan gum, agar, guar gum, ammonium acryloyldimethyltaurate/Vpicopolymer, dextrin, carbomer, dimethicone/vinyl Consisting of dimethicone crosspolymer, dimethicone crosspolymer, vinyl dimethicone, hydroxy methyl cellulose, hydroxy ethyl cellulose, hydroxy propyl methyl cellulose, sodium polyacryloyl dimethyl taurate, and combinations thereof It may be characterized by including one or more selected from the group.

Specifically, the antioxidant includes at least one selected from the group consisting of tocopheryl acetate, sodium ascorbyl phosphate, pentaerythrityltetra-di-t-butylhydroxyhydrocinnamate, and combinations thereof. can be characterized.

Specifically, the chelating agent is disodium EDTA, sodium diphosphate, sodium triphosphate, 1-hydroxyethane-1,1-diphosphoric acid, nitrilotrimethylenephosphonic acid, nitrilotriacetic acid, ethylene It may be characterized in that it includes at least one selected from the group consisting of diaminetetraacetate, 1,2,3,4-cyclopentanetetracarboxylic acid, and combinations thereof.

According to the present invention, when applied to the skin, it has excellent antioxidant activity and anti-inflammatory activity without causing safety problems such as irritation and redness as well as cytotoxicity, and has a rejuvenating effect by promoting skin moisturizing, skin regeneration, and skin elasticity can represent

In addition, the serum-type cosmetic composition according to the present invention has high skin absorption, so it quickly provides a moisturizing feeling to the skin, but does not show a heavy finish, so it has an excellent feeling of use, and can quickly soothe and improve problem skin.

However, the effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

1 is a graph showing the results of comparison of percutaneous permeability by type of sodium DNA containing polydeoxyribonucleotide among the components of a cosmetic composition according to an embodiment of the present invention.
2 (a) to (d) are graphs showing the rejuvenating effect of sodium DNA containing polydeoxyribonucleotide among the components of a cosmetic composition according to an embodiment of the present invention.
3 (a) and (b) are skin images showing the rejuvenating effect of sodium DNA containing polydeoxyribonucleotide among the components of a cosmetic composition according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail with reference to embodiments and drawings of the present invention. These examples are only presented as examples to explain the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples. .

In addition, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of skill in the art to which this invention belongs, and in case of conflict, this specification including definitions of will take precedence.

In order to clearly explain the proposed invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification. And, when a certain component is said to "include", this means that it may further include other components without excluding other components unless otherwise stated. Also, a “unit” described in the specification means one unit or block that performs a specific function.

In each step, the identification code (first, second, etc.) is used for convenience of description, and the identification code does not describe the order of each step, and each step does not clearly describe a specific order in context. It may be performed differently from the order specified above. That is, each step may be performed in the same order as specified, may be performed substantially simultaneously, or may be performed in the reverse order.

As used herein, the term "extract" refers to an extract obtained by extraction of raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, The extract itself and extracts of all formulations that can be formed using the extract may be included. The extract of the present invention may be extracted from natural, hybrid or mutant plants of each corresponding plant, and may also be extracted from plant tissue culture.

In the extraction of the raw material of the present invention, a method for extracting the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods.

In the present invention, the type of extraction solvent used to extract the raw material is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as ethyl acetate; Or a mixture thereof may be used, and preferably, purified water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more.

In the present invention, the extract obtained by hot water extraction or cold brewing is filtered to remove floating solid particles, for example, by filtering out particles using nylon or the like, or by using cryofiltration, and then used as is or freeze-dried or hot-air-dried. , spray drying, etc. can be used after drying.

Hereinafter, embodiments and embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. However, it may not be limited to these implementations and examples and figures herein.

One aspect of the present application, a solvent containing purified water, 1,2-hexanediol, diethoxyethyl succinate, and t-butyl alcohol; polyols including caprylyl glycol, butylene glycol, dipropylene glycol, and glycerin; allantoin; arginine; polydecene; calcium carbonate; An extract containing at least one selected from the group consisting of Lamiaceae extract, edelweiss callus culture extract, star anise extract, hydrolyzed golden snake nest extract, bladderwrack extract, and combinations thereof; emulsifier; It provides a serum-type cosmetic composition comprising a; and non-polar moisturizing oil.

The serum-type cosmetic composition according to the present invention has excellent antioxidant activity and anti-inflammatory activity without causing safety problems such as irritation and redness as well as cytotoxicity when applied to the skin, and promotes skin moisturizing, skin regeneration, and skin elasticity. By doing so, a rejuvenating effect can be exhibited. In addition, it has a high skin absorption power, so it has an excellent feeling of use because it does not show a heavy finish while quickly providing a moisturizing feeling to the skin, and has the advantage of quickly soothing and improving problematic skin.

In one embodiment, the cosmetic composition contains at least one selected from the group consisting of Lamiaceae extract, edelweiss callus culture extract, star anise extract, hydrolyzed golden snake nest extract, bladderwrack extract, and combinations thereof By including the extract, pharmacological effects of the extract can be exerted. Preferably, the cosmetic composition may include all of Lamiaceae extract, edelweiss callus culture extract, star anise extract, hydrolyzed pigeon nest extract, and bladderwrack extract.

In one embodiment, Lamiaceae extract, which is one of the components of the cosmetic composition, may mean that it is extracted from the outpost of Lamiaceae. The Lamiaceae extract can soothe problem skin by containing rosemary acid, urolic acid, rutin, etc. as active ingredients, and can exhibit a rejuvenating effect by promoting the synthesis of collagen and elastin. In addition, it can help prevent deterioration and decay of cosmetics.

If the Lamiaceae extract is included in less than about 0.0001% by weight, the effect resulting from the inclusion of the Lamiaceae extract may not be sufficiently exhibited, and if it exceeds about 0.01% by weight, mixing with other ingredients may be impaired. .

In one embodiment, the edelweiss callus culture extract, one of the constituents of the cosmetic composition, cut all or part of the stem, flower or leaf of edelweiss, one of the alpine plants native to the European Alps, cultured and extracted in an induction medium It can mean what was obtained through. The edelweiss callus culture extract exhibits high antioxidant and anti-inflammatory effects, and thus can exert a rejuvenating effect when included in a cosmetic composition.

Here, induction of culture relates to plant tissue culture, and is to propagate plant cells, organs, and plants by asepticly culturing living plant tissues in vitro. Since plant tissue culture uses the basic characteristics of plants, it is based on the ability to regenerate plants from somatic cells of plants, which is a unique feature of plants.

In one embodiment, the callus culture refers to a plant cell culture, which occurs when tissue cut from a plant is cultured in a medium containing auxin, or when some kind of plant is injured or the upper part is treated with auxin. It means a special tissue or cell mass. Callus is an amorphous tissue or cell mass that has lost the ability to induce normal organogenesis or tissue differentiation, and is mostly composed of vegetative cells, and may also include plant tumor tissue.

In one embodiment, the edelweiss callus culture extract is prepared by putting dried edelweiss callus in purified water, performing primary extraction while stirring at about 50 ° C. for about 8 hours, and hot water extraction at about 98 ° C. for about 10 minutes, or ultrasonic It may be extracted using an extraction method or may be a fermentation extract.

In one embodiment, the edelweiss callus culture extract may be included in about 0.01 to 0.1% by weight of the cosmetic composition. If the Edelweiss callus culture extract is included in less than about 0.01% by weight, the effect resulting from the inclusion of the Edelweiss callus culture extract may not be sufficiently exhibited, and if it exceeds about 0.1% by weight, mixability with other components may be reduced. may be hindered.

In one embodiment, the star anise extract, which is one of the components of the cosmetic composition, may mean an extract from star anise, which is mainly used as a spice and is also called octagonal fennel. As the star anise extract contains anethol as an active ingredient, it can remove substances harmful to the skin and protect the skin from harmful oxygen to improve problem skin and stabilize inflammatory reactions to exhibit a rejuvenating effect.

In one embodiment, the star anise extract may be included in about 0.01 to 0.1% by weight of the cosmetic composition. If the star anise extract is included in an amount of less than about 0.01% by weight, the effect resulting from the inclusion of the star anise extract may not be sufficiently exhibited, and if it exceeds about 0.1% by weight, mixing with other ingredients may be impaired. can

In one embodiment, the hydrolyzed gold snake nest extract, which is one of the components of the cosmetic composition, is obtained by treating a nest made by a gold snake, also called sea nest or swift, with an acid, enzyme, or other hydrolysis method may mean an extract. The hydrolyzed chinensis extract is rich in protein and amino acids, so it can not only promote skin collagen synthesis, but also help promote fibroblasts, showing skin regeneration and anti-wrinkle effects, and exhibiting anti-inflammatory effects, resulting in a rejuvenating effect can represent

In one embodiment, the hydrolyzed chinen nest extract may be included in the cosmetic composition in an amount of about 0.001 to 0.1% by weight. If the hydrolyzed golden snake nest extract is included in less than about 0.001% by weight, the effect shown by including the hydrolyzed golden snake nest extract may not be sufficiently exhibited, and if it exceeds about 0.1% by weight, other Mixability with ingredients may be impaired.

In one embodiment, the bladderwrack extract, which is one of the components of the cosmetic composition, may mean that it is extracted from bladderwrack, a type of seaweed. The bladderwrack extract is rich in calcium, iodine, magnesium, potassium, sodium, zinc, vitamins as well as minerals, and contains prolotanin and fucoxanthin that lower oxidative stress, thereby exhibiting high antioxidant and anti-inflammatory effects.

In one embodiment, the bladderwrack extract may be included in about 0.0001 to 0.01% by weight in the cosmetic composition. If the bladderwrack extract is included in an amount of less than about 0.0001% by weight, the effect resulting from the inclusion of the bladderwrack extract may not be sufficiently exhibited, and if it exceeds about 0.01% by weight, mixing with other components may be inhibited. can

In one embodiment, the solvent containing the purified water, 1,2-hexanediol, diethoxyethyl succinate, and t-butyl alcohol may be included in the cosmetic composition at about 85 to 95% by weight. If the solvent is included in less than about 85% by weight, the components may not be sufficiently dissolved, and if it exceeds about 95% by weight, the rejuvenating effect of the cosmetic composition may not be sufficiently exhibited.

In one embodiment, the polyol containing caprylyl glycol, butylene glycol, dipropylene glycol, and glycerin is included for the skin moisturizing and skin improving effect of the cosmetic composition, It may be included in about 1 to 10% by weight. If the polyol is included in less than about 1% by weight, the effect resulting from the inclusion of the polyol may not be sufficiently exhibited, and if it exceeds about 10% by weight, the feeling of use may decrease.

In one embodiment, allantoin is a substance extracted from the shoots of comfrey and wheat, which are annual grasses, and exhibits high anti-inflammatory efficacy, so that when included in the cosmetic composition, allantoin can exert a skin soothing and alleviating effect.

In one embodiment, the allantoin may be contained in about 0.01 to 01% by weight of the cosmetic composition. If the allantoin is included in less than about 0.01% by weight, the skin soothing and alleviating effect due to the allantoin may not be sufficiently exhibited, and if it exceeds about 0.1% by weight, mixing with other ingredients may be inhibited.

In one embodiment, the arginine is a natural amino acid, which is added to the cosmetic composition to serve to fix moisture, thereby preventing the skin from drying out and helping to keep it moist. The arginine may be included in an amount of about 0.0000001 to 0.0001% by weight in the cosmetic composition, and if the arginine is included in an amount of less than about 0.0000001% by weight, the effect of including the arginine may not be sufficiently exhibited, When it exceeds about 0.0001% by weight, miscibility with other components may be impaired.

In one embodiment, the polydecene may be added to the cosmetic composition to exhibit an effect of blocking moisture evaporation. The polydecene may be included in about 1 to 10% by weight in the cosmetic composition, and if the polydecene is included in less than about 1% by weight, the effect of including the polydecene may not be sufficiently exhibited, If it exceeds about 10% by weight, mixability with other components may be impaired.

In one embodiment, the calcium carbonate may be added to the cosmetic composition to exhibit an opacifying effect with a pH buffering effect. The calcium carbonate may be included in about 0.0000001 to 0.0001% by weight in the cosmetic composition, and if the calcium carbonate is included in less than about 0.0000001% by weight, the effect of including the calcium carbonate may not be sufficiently exhibited, If it exceeds about 0.0001% by weight, skin irritation may appear strong.

In one embodiment, the emulsifier is added to easily mix the components included in the cosmetic composition, for example, lysolecithin, glyceryl stearate, polyglyceryl-2 stearate, hydrogenity consisting of drape seed alcohol, stearic acid, stearyl alcohol, caprylyl/capryl glucoside, hydrogenated lecithin, hydrolyzed jojoba ester, glyceryl caprylate, alkylamidoalkylamine, and combinations thereof It may contain one or more selected from the group.

Preferably, the emulsifier may include both lysolecithin and hydrogenated lecithin, but is not limited thereto.

In one embodiment, lysolecithin and hydrogenated lecithin included in the emulsifier may be included in an amount of about 0.001 to 0.1% by weight, respectively, in the serum-type cosmetic composition. If lysolecithin and hydrogenated lecithin included in the emulsifier are included in an amount of less than about 0.001% by weight, respectively, the components of the cosmetic composition may not be easily mixed with each other, resulting in aggregation or sinking, and about 0.1% by weight Excessive foaming may occur. For example, lysolecithin and hydrogenated lecithin included in the emulsifier may be included in about 0.001 to 0.1% by weight, about 0.001 to 0.01% by weight, or about 0.01 to 0.1% by weight of the serum-type cosmetic composition, respectively. Not limited to this.

In one embodiment, the non-polar moisturizing oil refers to hydrocarbon oils consisting only of hydrocarbons, and at least one of them is selected and used. For example, it may include one or more selected from the group consisting of hydrogenated olefin polymer, squalane, hydrogenated polydecene, caprylic/capric triglyceride, dicaprylyl ether, and combinations thereof. . The non-polar moisturizing oil may exhibit skin elasticity improvement and skin soothing effects along with an excellent moisturizing effect, and thus may exhibit a rejuvenating effect by being added to the cosmetic composition. Preferably, the non-polar moisturizing oil may include caprylic/capric triglyceride, but is not limited thereto.

In one embodiment, the caprylic / capric triglyceride may be included in the cosmetic composition in about 0.01 to 1% by weight. If the non-polar moisturizing oil is included in an amount of less than about 0.01% by weight, the effects of moisturizing, improving skin elasticity, and soothing the skin may not be sufficiently exhibited due to the inclusion of the non-polar moisturizing oil, and if it exceeds about 1% by weight, the layer Separation may occur.

In one embodiment, the cosmetic composition may further include one or more additives selected from the group consisting of preservatives, skin conditioning agents, thickeners, antioxidants, chelating agents, defoaming agents, pigments, and combinations thereof. can

In one embodiment, the skin conditioning agent consists of sodium DNA, sodium hyaluronate, ethylhexylglycerin, copper tripeptide-1, glycereth-25 PCA isostearate, adenosine, and combinations thereof. It may contain one or more selected from the group.

Preferably, the skin conditioning agent may include all of sodium DNA, sodium hyaluronate, ethylhexyl glycerin, copper tripeptide-1, glycereth-25 PCA isostearate, and adenosine.

In one embodiment, sodium DNA, sodium hyaluronate, ethylhexyl glycerin, copper tripeptide-1, glycereth-25 PCA isostearate, and adenosine included in the skin conditioning agent are the serum-type cosmetic In the composition, each may be included in about 0.000001 to 1% by weight. If sodium DNA, sodium hyaluronate, ethylhexyl glycerin, copper tripeptide-1, glycereth-25 PCA isostearate, and adenosine contained in the skin conditioning agent are each less than about 0.000001% by weight Moisturizing, skin elasticity improvement, and skin soothing effects that appear according to the inclusion of the skin conditioning agent may not be sufficiently exhibited, and when it exceeds about 1% by weight, mixing with other ingredients may be inhibited. For example, sodium DNA, sodium hyaluronate, ethylhexyl glycerin, copper tripeptide-1, glycereth-25 PCA isostearate, and adenosine included in the skin conditioning agent are each about 0.000001 to 1% by weight, about 0.000001 to 0.1% by weight, about 0.000001 to 0.01% by weight, about 0.000001 to 0.001% by weight, about 0.000001 to 0.0001% by weight, about 0.000001 to 0.00001% by weight, about 0.000001 to 1% by weight to 0.1 wt%, about 0.00001 to 0.01 wt%, about 0.00001 to 0.001 wt%, about 0.00001 to 0.0001 wt%, about 0.0001 to 1 wt%, about 0.0001 to 0.1 wt%, or about 0.0001 to 0.01 wt%, about 0.0001 to 0.001% by weight, about 0.001 to 1% by weight, about 0.001 to 0.1% by weight, about 0.001 to 0.01% by weight, about 0.01 to 1% by weight, about 0.01 to 0.1% by weight, or about 0.1 to 1% by weight; , but not limited thereto.

In one embodiment, sodium DNA, one of the components included in the cosmetic composition, may contain polydeoxyribonucleotide (PDRN) as a main active ingredient. The polydeoxyribonucleotide is a low-molecular-weight DNA complex extracted from sodium DNA of sturgeon testis, and refers to an injectable pharmaceutical ingredient approved by the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA). The polydeoxyribonucleotide exhibits high safety and stability, so it does not show side effects on the skin, is absorbed quickly, can induce angiogenesis by promoting VEGF, and can selectively bind to the A2 receptor to exhibit anti-inflammatory effects It exhibits anti-thermal properties, is not destroyed by high-temperature sterilization, maintains pharmacological activity, secretes growth factors to activate fibroblasts, etc., and has an excellent regeneration effect of damaged skin. In addition, polydeoxyribonucleotides used in the present invention can have very high purity by including those extracted from live sturgeon testicles, unlike polydeoxyribonucleotides extracted from eggs or testis of dead salmon.

In one embodiment, the polydeoxyribonucleotide may be included in the form of a transfersome prepared using an encapsulation system. As the polydeoxyribonucleotides are included in the form of transfersomes, they have higher flexibility than general liposomes and can be subjected to extreme deformation, so that they can easily penetrate through small gaps in the horny layer of the skin.

In one embodiment, the thickener is acrylate / C10-30 alkyl acrylate crosspolymer, xanthan gum, agar, guar gum, ammonium acryloyldimethyltaurate / Vpicopolymer, dextrin, carbomer, dimethine Thicone/vinyldimethicone crosspolymer, dimethicone crosspolymer, vinyldimethicone, hydroxy methylcellulose, hydroxyethylcellulose, hydroxypropylmethylcellulose, sodium polyacryloyl dimethyltaurate, and combinations thereof It may include one or more selected from the group consisting of.

In one embodiment, the thickener is added to improve the feeling of use and skin moisturizing power of the cosmetic composition, preferably xanthan gum, agar, guar gum, ammonium acryloyldimethyltaurate / Vpicopolymer, It may include both dextrin and hydroxypropylmethylcellulose.

In one embodiment, xanthan gum, agar, guar gum, ammonium acryloyldimethyltaurate / Vpicopolymer, dextrin, and hydroxypropylmethylcellulose included in the thickener are each about 0.0000001 to 1 in the cosmetic composition It may be included in weight percent. If xanthan gum, agar, guar gum, ammonium acryloyldimethyltaurate/Vpicopolymer, dextrin, and hydroxypropylmethylcellulose contained in the thickener are each less than about 0.0000001% by weight, the thickening effect is not sufficiently exhibited. It may not be, and when about 1% by weight is exceeded, the feeling of use may rather decrease. For example, xanthan gum, agar, guar gum, ammonium acryloyldimethyltaurate/Vpicopolymer, dextrin, and hydroxypropylmethylcellulose included in the thickener are each about 0.0000001 to 1% by weight in the cosmetic composition. , about 0.0000001 to 0.1 wt%, about 0.0000001 to 0.01 wt%, about 0.0000001 to 0.001 wt%, about 0.0000001 to 0.0001 wt%, about 0.0000001 to 0.00001 wt%, about 0.0000001 to 0.00000 0.0000001 to 0.00000 0,01 wt% About 0.000001 to 0.1% by weight, about 0.000001 to 0.001% by weight, about 0.000001 to 0.0001% by weight, about 0.000001 to 0.00001% by weight, about 0.00001 to 1% by weight, about 0.00001 to 0.1% by weight, about 0.00001 to 0. 0.00001 to 0.001 wt%, about 0.00001 to 0.0001 wt%, about 0.0001 to 1 wt%, about 0.0001 to 1 wt%, about 0.0001 to 0.1 wt%, about 0.0001 to 0.01 wt%, about 0.0001 to 0.001 wt%, about 0.001 to 1% by weight, about 0.001 to 0.1% by weight, about 0.001 to 0.01% by weight, about 0.01 to 1% by weight, about 0.001 to 0.1% by weight, or about 0.1 to 1% by weight, but is not limited thereto.

In one embodiment, the antioxidant is at least one selected from the group consisting of tocopheryl acetate, sodium ascorbyl phosphate, pentaerythrityltetra-di-t-butylhydroxyhydrocinnamate, and combinations thereof. It may contain, preferably it may contain sodium ascorbyl phosphate.

In one embodiment, the sodium ascorbyl phosphate may be included in about 0.001 to 0.01% by weight of the cosmetic composition. If the sodium ascorbyl phosphate is less than about 0.001% by weight, respectively, the antioxidant effect may not be sufficiently exhibited, and if it exceeds about 0.01% by weight, skin irritation may occur.

In one embodiment, the chelating agent is disodium EDTA, sodium diphosphate, sodium triphosphate, 1-hydroxyethane-1,1-diphosphonic acid, nitrilotrimethylenephosphonic acid, nitrilotri It may contain at least one selected from the group consisting of acetic acid, ethylenediaminetetraacetate, 1,2,3,4-cyclopentanetetracarboxylic acid, and combinations thereof, preferably disodium idithio may include this.

In one embodiment, the chelating agent may be included in about 0.01 to 0.1% by weight of the cosmetic composition. If it is less than about 0.01% by weight, stability of the composition may decrease, and if it exceeds about 0.1% by weight, skin irritation may occur.

In one embodiment, the serum-type cosmetic composition may further include Portulaca oleracea extract as an additive.

As the additive, the portulaca extract means that it is extracted from portulaca, which is named to resemble a horse's teeth. The portulaca is also called purslane or jangmyeongchae, and can exhibit an effect of healing wounds and relieving irritation without exhibiting skin toxicity. Therefore, the serum-type cosmetic composition may exhibit skin irritation relieving and wound healing effects as it contains portulaca oleracea extract as an additive.

In one embodiment, the portulaca extract may be contained in about 0.01 to about 0.1% by weight. If the Portulaca oleracea extract is included in an amount of less than about 0.01% by weight, the irritation relief and wound healing effect resulting from the inclusion of the Portulaca oleracea extract may not be sufficiently exhibited, and if it exceeds about 0.1% by weight, the feeling of use may decrease.

In one embodiment, the serum-type cosmetic composition may improve formulation stability by further including L-arginine. The L-arginine is a pH adjusting agent, and formulation stability may be improved as the serum-type cosmetic composition includes about 0.001 to about 0.1% by weight of L-arginine. If the L-arginine is included in less than about 0.001% by weight, the effect of improving formulation stability may not be sufficiently exhibited, and if it exceeds about 0.1% by weight, manufacturing cost may be excessively increased.

In one embodiment, the serum-type cosmetic composition may further include a linseed mucus extract. The linseed mucus extract is excellent in promoting differentiation of skin keratinocytes, restoring skin barrier function, moisturizing effect, and improving the feeling of use, and thus, the cosmetic composition may exhibit a rejuvenating effect by including the linseed mucus extract.

In one embodiment, the linseed mucus extract may be obtained by a physical separation method such as scraping from linseed, according to a conventional method known in the art, that is, under normal temperature and pressure conditions, in a conventional solvent It can also be obtained in the form of an extract from flaxseed using.

In one embodiment, the linseed mucus extract may be contained in about 0.001 to about 0.01% by weight. If the linseed mucus extract is included in less than about 0.001% by weight, the rejuvenating effect may not be sufficiently exhibited, and if it exceeds about 0.01% by weight, the feeling of use may be reduced.

In one embodiment, the serum-type cosmetic composition may further include a dapssari fruit extract. The Dapsari fruit extract means that it is obtained by hot water extraction of the fruit of Dapsarii, an annual plant of the Pollackaceae family, in an aqueous ethanol solution. The Dapssari fruit extract exhibits a high moisturizing effect without causing skin irritation, so it can be added during the preparation of a cosmetic composition to give an effect that prevents skin from pulling.

In one embodiment, the dapssari fruit extract may be contained in about 0.01 to about 0.1% by weight. If the Dapssari fruit extract is included in less than about 0.01% by weight, the effect of including the extract may not be sufficiently exhibited, and if it exceeds about 0.1% by weight, mixing with other components may be inhibited. .

In one embodiment, the serum-type cosmetic composition may further include Dalbergia olive berry extract. The Dalbergia olive berry extract means obtained by hot water extraction of the outpost of Dalbergia Oliveri, a kind of rose tree found in Myanmar, Thailand, Laos, Cambodia and the like, in a dichloromethane solvent. The Dalbergia olive berry extract has an excellent fragrance and exhibits a skin moisturizing effect, so it can be used as a natural fragrance and skin protectant when used in cosmetic compositions.

In one embodiment, the dalbergia olive berry extract may be contained in about 0.01 to about 0.1% by weight. If the Dalbergia olive berry extract is included in an amount of less than about 0.01% by weight, the effect obtained by including the extract may not be sufficiently exerted, and if it exceeds about 0.1% by weight, skin irritation may occur.

In one embodiment, the above-described effect is exerted by organic interaction as all of the above-described components are included, and the above effect may not be achieved when the active ingredient is selectively included. .

In one embodiment, the cosmetic composition, in addition to the components described above, may additionally further include components typically required for preparing a cosmetic composition, but may not be limited thereto.

In one embodiment, the formulation of the cosmetic composition may be a serum or essence formulation in the form of a paste, cream, or gel.

In one embodiment, when the formulation of the cosmetic composition is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc as a carrier component Alternatively, zinc oxide may be included, but may not be limited thereto.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

[Experimental Example 1]

In order to confirm percutaneous permeability according to the form of sodium DNA containing polydeoxyribonucleotide as a main active ingredient, sodium DNA was synthesized in the form of liposomes and transfersomes, respectively. Transdermal permeability of the synthesized liposome sodium DNA and transfersome sodium DNA was measured, and the results are shown in FIG. 1 .

As shown in FIG. 1 below, it was confirmed that sodium DNA synthesized as a transfersome through an encapsulation system can exhibit a high skin absorption rate with a percutaneous permeability of about 18.3 times or more compared to sodium DNA synthesized as a liposome.

[Experimental Example 2]

The anti-aging effect of sodium DNA containing polydeoxyribonucleotide as a main active ingredient was measured. The measurement method was to apply a sodium DNA composition containing polydeoxyribonucleotide as the main active ingredient to 2 points around the eyes to 20 Caucasian women between the ages of 30 and 60, twice a day for 8 weeks. After weeks, skin moisture level, skin thickness, skin wrinkles, and skin elasticity were measured and shown in FIGS. 2 and 3 .

As shown in FIGS. 2 and 3, as a result of applying the sodium DNA composition containing polydeoxyribonucleotide as a main active ingredient to the skin, the moisture content of the skin increased by about 5%, and the thickness of the skin increased by about 8.7%. , The wrinkles of the skin were reduced by about 4% and the elasticity of the skin was increased by about 25.6% (FIG. 2). In addition, it was confirmed that a noticeable skin improvement effect was shown in the result of taking images of the skin (FIG. 3).

[Examples 1 to 3]

Serum-type cosmetic compositions of Examples 1 to 3 were prepared by uniformly mixing the ingredients listed in Table 1 below using an emulsifier.

[Comparative example]

The components listed in Table 1 below were uniformly mixed using an emulsifier to prepare a serum-type cosmetic composition, which was named a comparative example.

Ingredients Example 1 Example 2 Example 3 comparative example 1,2-Hexanediol 1.290200 1.290200 1.290200 1.500000 t-butyl alcohol 0.020000 0.020000 0.020000 0.020000 Diethoxyethylsuccinate 0.050000 0.050000 0.050000 0.000000 Purified water 88.892512 88.892512 88.892512 87.133000 glycerin 0.006550 0.006550 0.006550 0.010000 Dipropylene Glycol 2.000000 2.000000 2.000000 2.500000 Butylene Glycol 2.315000 2.315000 2.315000 2.500000 caprylyl glycol 0.120000 0.120000 0.120000 0.000000 Lysolecithin 0.001000 0.001000 0.001000 0.001000 Hydrogenated Lecithin 0.006001 0.006001 0.006001 0.000000 guar gum 0.112500 0.112500 0.112500 0.200000 dextrin 0.000001 0.000001 0.000001 0.000000 baby 0.060750 0.060750 0.060750 0.100000 xanthan gum 0.002500 0.002500 0.002500 0.000000 Hydroxypropyl Methyl Cellulose 0.000001 0.000001 0.000001 0.000000 honey grass extract 0.000500 0.000500 0.000500 0.000000 sodium ascorbyl phosphate 0.001100 0.001100 0.001100 0.010000 polydecene 4.045500 4.045500 4.045500 5.000000 calcium carbonate 0.000001 0.000001 0.000001 0.000000 Caprylic/Capric Triglyceride 0.020000 0.020000 0.020000 0.020000 Ammonium acryloyldimethyltaurate/VP copolymer 0.480000 0.480000 0.480000 0.500000 Bladder Wrack Extract 0.000500 0.000500 0.000500 0.000000 Star Anise Extract 0.030000 0.030000 0.030000 0.100000 arginine 0.000001 0.000001 0.000001 0.000000 Edelweiss Callus Culture Extract 0.098000 0.098000 0.098000 0.000000 Hydrolyzed Golden Snake Nest Extract 0.001000 0.001000 0.001000 0.000000 Disodium EDT 0.040020 0.040020 0.040020 0.050000 Glycereth-25PCA Isostearate 0.300000 0.300000 0.300000 0.300000 sodium DNA 0.010000 0.010000 0.010000 0.010000 sodium hyaluronate 0.005501 0.005501 0.005501 0.005000 allantoin 0.050500 0.050500 0.050500 0.000000 Ethylhexylglycerin 0.000360 0.000360 0.000360 0.001000 Copper tripeptide-1 0.000005 0.000005 0.000005 0.000000 adenosine 0.040000 0.040000 0.040000 0.040000 L-Arginine 0.000000 0.000000 0.050000 0.000000 Dalbergia Olive Berry Extract 0.000000 0.010000 0.010000 0.000000 Dabshari fruit extract 0.000000 0.008000 0.008000 0.000000 portulaca extract 0.000000 0.000000 0.030000 0.000000 Linseed mucus extract 0.000000 0.020000 0.020000 0.000000 Sum 100.00 100.00 100.00 100.00

[Experimental Example 1: Measurement of MMP-1 production]

For the serum-type cosmetic compositions prepared in the above Examples and Comparative Examples, the amount of matrix metalloproteinase-1 (MMP-1) gene involved in wrinkle formation was measured. First, after dispensing and culturing HDFa cells at a concentration of 1×10 ⁴ cells/well in a 6 well plate, a control medium was used, 2% of each cosmetic composition sample was treated, and cultured for 12 hours. Thereafter, after culturing for 12 hours by treatment with 200 mJ UVB, the culture medium was centrifuged at 800 rpm for 5 minutes, and the amount of production was measured using the Human Pro-MMP-1 Quantikine ELISA Kit. The results are shown in Table 2 below.

division UVB Treatment concentration (w/v%) MMP-1 production (%) Control - - 100 Negative control + - 112.1 Example 1 + 2.0 87.6 Example 2 + 2.0 81.4 Example 3 + 2.0 77.7 Comparative Example 3 + 2.0 97.4

As confirmed in Table 2, in the case of the serum-type cosmetic composition according to the embodiment, compared to the cosmetic composition of the comparative example, it exhibited an excellent MMP-1 production inhibitory effect.

[Experimental Example 2: Stability Evaluation]

The stability of the serum-type cosmetic compositions prepared in Examples and Comparative Examples was evaluated. In the stability evaluation, it was visually determined whether component separation occurred when the prepared cosmetic compositions were left at room temperature (20° C.) or above room temperature (35° C.) for 3 months, respectively. The criterion was described as 'unstable' when the separation of components occurred, and 'stable' when the separation did not occur. The stability evaluation results are shown in Table 3 below.

temperature Example 1 Example 2 Example 3 comparative example 20℃ stability stability stability stability 35℃ stability stability stability Instability

As shown in Table 3, the serum-type cosmetic composition of Examples according to the present invention, unlike the cosmetic composition of Comparative Example, in which separation occurred when left at a temperature of 35 ° C. for 3 months, was separated even during long-term storage at room temperature and room temperature or higher. It was confirmed that it was stable without any symptoms.

[Experimental Example 3: Sensory Evaluation]

prepared in the above Examples and Comparative Examples Satisfaction including skin moisture absorption rate, moisture retention, skin regeneration and skin soothing effect of the serum-type cosmetic composition was confirmed through evaluation. The experiment was conducted on 90 men and women between the ages of 30 and 45 who met the selection criteria and were not included in the exemption criteria. They were divided into 15 people each and applied once a day for 4 weeks as a self-application method. The degree of preference felt by the subject was evaluated. The evaluation criteria were very good (5 points), excellent (4 points), average (3 points), poor (2 points), and very poor (1 point). Table 4 shows.

evaluation item Example 1 Example 2 Example 3 comparative example moisturizing skin 3.8 4.2 4.5 2.5 skin softness 4.0 4.5 4.7 3.2 No skin irritation 4.8 5.0 5.0 3.5 soothe skin 4.0 4.2 4.3 2.1 skin regeneration 3.2 3.5 3.8 1.5 product satisfaction 4.1 4.3 4.6 2.8

As shown in Table 4, in the case of the serum-type cosmetic composition prepared according to the present invention, unlike the cosmetic composition of Comparative Example, most of the subjects showed overall skin moisturization, absorption rate, skin regeneration and skin soothing, non-irritation, etc. Satisfaction was rated above average.

In this specification, only a few examples of various embodiments performed by the present inventors are described, but the technical spirit of the present invention is not limited or limited thereto, and can be modified and implemented in various ways by those skilled in the art, of course.

Claims (8)

85 to 95% by weight of a solvent including purified water, 1,2-hexanediol, diethoxyethylsuccinate, and t-butyl alcohol;
1 to 10% by weight of a polyol including caprylyl glycol, butylene glycol, dipropylene glycol, and glycerin;
0.01 to 0.1% by weight of allantoin;
0.0000001 to 0.0001% by weight of arginine;
1 to 10% by weight of polydecene;
0.0000001 to 0.0001% by weight of calcium carbonate;
Lamiaceae extract 0.0001 to 0.01% by weight, edelweiss callus culture extract 0.01 to 0.1% by weight, star anise extract 0.01 to 0.1% by weight, hydrolyzed pigeon nest extract 0.001 to 0.1% by weight, bladderwrack extract 0.0001 to 0.01% by weight, Dalber An extract containing 0.01 to 0.1% by weight of Gia olive berry extract, 0.01 to 0.1% by weight of Dapsaria fruit extract, and 0.001 to 0.01% by weight of linseed mucus extract;
0.001 to 0.01% by weight of an emulsifier including lysolecithin and hydrogenated lecithin;
0.01 to 1% by weight of caprylic/capric triglyceride;
0.0001 to 0.01% by weight of xanthan gum, 0.01 to 0.1% by weight of agar, 0.1 to 1% by weight of guar gum, 0.1 to 1% by weight of ammonium acryloyldimethyltaurate/Vpicopolymer, 0.000001 to 0.00001% by weight of dextrin, and hydride A thickener containing 0.000001 to 0.00001% by weight of hydroxypropylmethylcellulose;
0.001 to 0.01% by weight of sodium ascorbyl phosphate; and
0.01 to 0.1% by weight of disodium EDTA;
Containing, a serum-type cosmetic composition.
delete delete According to claim 1,
Characterized in that it further comprises a skin conditioning agent, a serum-type cosmetic composition.
The method of claim 4, wherein the skin conditioning agent,
Characterized in that it comprises at least one selected from the group consisting of sodium DNA, sodium hyaluronate, ethylhexyl glycerin, copper tripeptide-1, glycereth-25 PCA isostearate, adenosine, and combinations thereof To, a serum-type cosmetic composition.
delete delete delete

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