KR20030064059A - Cosmetic composition containing paeonia suffruticosa andrews extract and albizzia julibrissin dura extract having anti-ageing effect - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Paeonia suffruticosa Andrews extract and an Albizzia julibrissin Duraz. extract having anti-aging effect is provided. Therefore, the composition has excellent skin elasticity-enhancing effect, antioxidant effect, hyaluronase inhibiting effect, product stability and safety to the skin. CONSTITUTION: An anti-aging cosmetic composition contains a Paeonia suffruticosa Andrews extract and an Albizzia julibrissin Duraz. extract in a ratio of 1:0.001 to 1:20. The extract of Paeonia suffruticosa Andrews and Albizzia julibrissin Duraz. is obtained by extracting in conventional solvents, preferably a mixture of C1-4 anhydrous or hydrous lower alcohol, propylene glycol and distilled water in a ratio of 2:1:1 at 60 to 85deg.C for 5 to 7hr. The use of two herbal extracts having effective anti-aging and antioxidant effects exhibits a synergistic prevention effect on skin aging.

Description

Anti-aging cosmetic composition containing the extract of the bark and the extract of Albizia julibrissin

The present invention relates to a cosmetic composition having an anti-aging effect, and more particularly, to an anti-aging cosmetic composition containing a extract of bark and extracts.

As for the skin aging mechanism that is the basis of anti-aging cosmetics development, the study of local exogenous photoaging, which is progressed by long-term ultraviolet exposure in parallel with systemic and endogenous physiological aging according to the increase of age, has been focused. In particular, the effects of photoaging on the formation of wrinkles on the face, head, etc., which are important for beauty, have been found to be large, and research on photoaging and wrinkle formation using human skin or animal models has been actively conducted as a basic research for developing anti-aging cosmetics. It is becoming.

As a change in the internal structure of the skin due to photoaging, epidermis, thickening of the dermis, accumulation of elastin-like substances, or the change of the three-dimensional structure of the elastic fibers have been reported. In addition, in relation to collagen, which is a major constituent skeleton of skin structure, microfiberization and weakening of fibrous form of collagen fibers have been revealed (Zheng P. and Kligman LH, uvA-induced ultrastructural changes in hairless mouse skin: A comparison to uvB-induced damage., J. Invest.dermatol., 100, 194-199 (1993))

However, the collagen fiber bundles formed by binding these are known to have a great influence on the toughness of the skin through the study of skin wound healing process, etc., but the effects on the structural change, the physical properties of the skin and the aging formation due to photoaging There has not been enough review so far.

The mainstream of the recent skin aging research is the study on the direct and indirect effects of sunlight due to the destruction of the ozone layer, and the effects on the collagen fiber using various substances to measure the effect of wrinkle formation caused by the light. Is actively researching.

The albizzia julibrissin Dura, on the other hand, is also called cassia, wild beast, lignum, or lumber. The leafy branch is called a tall tree, and the leaf is a double leaf with a knife-like small leaf. The leaves stick together at night and spread out during the day. Pink flowers gather at the ends of branches, and the fruit is shaped like a bean pod. It grows in mountains and fields 500 ~ 700m above sea level in central and south, and is planted around parks and houses in various places. In the evening, because the leaves are facing each other, called Yahab, marriage. Ingredients include alkaloids, tannins and saponins in the bark, cuercitrin in the leaves, vitamin C in the young leaves, alkaloids in the seeds, and glycoside albirosine. Pharmacological effects include sedatives, urinary medicines, pain medicines, insect insect medicines, when the end of the tooth is sore and unable to sleep, it is used for fractures and swellings (primary Korean herbal painting, Kyohaksa, 1999).

In this regard, Korean Patent Application Publication No. 84-3418 discloses an anticancer agent and leukemia treatment drug using a liquid selected from a hot body after maintaining a silk tree for 30 days after a predetermined treatment process, and A manufacturing method is disclosed.

In addition, Paeonia suffruticosa Andrews is also called Peony, Peony, Peony. The height of the tree is about 1.5m, the leaves are 1 ~ 2 times 3 leafs, and the leaves are 3 ~ 5 pieces. Large flowers bloom early in the summer, and the flowers come in many varieties. Root bark and cores include phenonol glycosides, phonosides (phenonol-β-D-glucoside), phenonides (phenonol-β-rhamnoglucoside), and peonyflorin. Others include benzoic acid, β-sitosterol, camphorsterol, and phenool. Phenolic glycosides are decomposed when the drug is stored, forming crystals of pheonol (C ₉ H ₁₀ O ₃ : melting point 52 ° C, d ₁₈ 1.310, n ₁₈ 1.5432, soluble in alcohol, ether, chloroform) on the surface of the medicine do. Total pheonol content is from 1.8 to 1.9% in the root bark. There is no pheonol in the stem bark, but there are pheonolides and peonyflorin. Pharmacological substances are reported as pheonol and its glycosides, and they have central inhibitory effects such as sedation, heat lowering, soreness, and myrrhosis, as well as anti-inflammatory and bloody action ( Use, Membership, 1984).

In this regard, Korean Patent Application Publication No. 2000-0067187 discloses a method for manufacturing cosmetics by extracting woody skin and adding it to cosmetics after a predetermined treatment process, Japanese Patent Application Laid-Open No. 10-306031, 06-279256. No. 10-310532 discloses herbal preparations and external skin preparations containing the extracts.

However, the cosmetics containing only the extract of the bark as described above, although the antioxidant effect, the anti-aging effect, etc. can be implemented to some extent, but the effect is not so excellent there is a problem in that it does not actually see a great effect.

The present inventors have tried to mix with various substances based on the bark skin extract in order to obtain a cosmetic having a more effective anti-aging effect, and found that synergistic effect of anti-aging appears when combining the extract of the almond tree to complete the present invention. It was.

Therefore, an object of the present invention is to obtain a cosmetic composition excellent in antioxidant and anti-aging effects.

In order to achieve the above object, according to the present invention, anti-aging cosmetics, characterized in that it contains 0.001 to 20% by weight of the total weight of the composition of the mixture of the bark skin extract and the extract of the silk tree in a weight ratio of 1: 0.001 to 1:20 A composition is provided.

Hereinafter, the content of the present invention will be described in more detail.

To obtain the bark extract, root bark, bark and stem bark of bark bark may be used, and to obtain bark tree extract, bark, leaves and seeds of the bark tree may be used.

Bark extract and silk tree extract are obtained as follows.

Bark bark and silk tree are pulverized after complete drying, respectively, and extracted by adding an extraction solvent to the pulverized product. As the extraction solvent, a conventional extraction solvent may be used, and a mixed solution of anhydrous or hydrous lower alcohol, propylene glycol, and distilled water having 1 to 4 carbon atoms is preferable, and these are mixed and used in a weight ratio of 2: 1: 1. Is particularly preferred. At this time, the ratio of the pulverized product and the extraction solvent mixture is set to a weight ratio of 1:10 to 1:30, preferably to a weight ratio of 1:20. It is extracted for 5-7 hours at 60-85 ° C. in an extractor equipped with a reflux condenser and filtered to remove insoluble matters. It was concentrated under reduced pressure, and then lyophilized to obtain extracts of the bark and the extract of Albizia julibrissin.

Subsequently, the extract of the bark and the silk tree obtained as described above are mixed. The mixing ratio of the bark bark extract and the silk tree extract is 1: 0.001 to 1:20 by weight, preferably 1: 0.01 to 1:10 by weight. When the ratio of the silk tree extract to the bark extract is lower than 0.001, no obvious anti-aging effect can be expected, and when the ratio is higher than 20, the effect increase due to the increase in content in terms of anti-aging and product stability is insignificant.

The mixture can be added to all cosmetics related to anti-aging, such as softening cream, nutrient cream, nourishing cream, essence, massage cream, etc. The amount is 0.001-20% by weight, preferably 0.01-10% by weight, based on the total weight of the cosmetic composition. It is enough. If the added amount is less than 0.001% by weight can not be expected anti-aging effect, in the case of more than 20% by weight it is uneconomical because there is no significant change in the anti-aging effect and product stability even if the content is increased.

Hereinafter, the present invention will be described in detail with reference to Examples. However, these examples are for illustrating the present invention, and the present invention is not limited to these examples.

Example

(Preparation of Bark Extract)

Commercially available bark skins were dried in a cool place free from sunlight until there was no change in weight, and then ground with a homogenizer (AM-7, Hyronchem).

To 50 g of the pulverized product, 1000 g of a mixed solution mixed with methanol, propylene glycol and distilled water in a weight ratio of 2: 1: 1 were added, and the mixture was extracted at 75 ° C for 5 hours. Filtration was carried out with a 200 mesh filter cloth (Cheongggye Co., Ltd.), and the extract was filtered through whatman No. 2 filter paper at room temperature to remove insoluble matters. This was concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling capacitor and then lyophilized to obtain a dry weight of 7.5 g of the bark extract.

(Preparation of Alder Extract)

In the same manner as the method for preparing the extract of bark skin, a weight of 8.2 g of the extract of Albizia julibrissin was obtained.

(Example 1)

The extract obtained from the bark and the extract of Albizia julibrissin were mixed in a weight ratio of 1: 0.5 using a stirrer (Songdang parts company, SSD-811A).

(Example 2)

The obtained bark bark extract and the extract of Albizia julibrissin were mixed in a weight ratio of 1: 1 using a stirrer (Songdang parts company, SSD-811A).

(Comparative Examples 1 and 2)

The obtained bark bark extract and silk tree extract were Comparative Examples 1 and 2, respectively.

(Test Example 1)

Antioxidative effect

Antioxidant effect was measured by thiobutylate acetic acid (TBA) test using lipid peroxidation system by Fenton reaction.

0.1 ml of dodecylmethyl sulfate solution in which 0.01 g of the extracted sample was dissolved in 5 ml of 25 mM Tris buffer / 0.75 mM potassium buffer containing 10 µl of ethyl linoleate, 0.2% dodecyl sulfate, 10 mol of ferric chloride, and 2 mol of hydrogen peroxide. Was added. After 16 hours of incubation at 55 ° C, 50 µl of 4% butylhydroxy toluene was added and the reaction was stopped. 0.3 ml of this solution was placed in a test tube, 3.0 ml of 0.05 M hydrochloric acid and 4.0 ml of butanol were added thereto, and then the absorption wavelength was measured at 535 nm. The antioxidant effect was calculated by the following formula compared with the control without the sample.

The results are shown in Table 1 below.

sample Antioxidative effect Bark skin extract of Comparative Example 1 50.5 Silkworm Extract of Comparative Example 2 35.8 Bark skin extract + silk extract of Example 1 (weight ratio 1: 0.5) 76.4 Bark bark extract + silk tree extract of Example 2 (weight ratio 1: 1) 79.7 Vitamin E 43.1

As shown in Table 1, the sample combined with the extract of Albizia julibrissin showed the superior antioxidant effect than the other samples.

(Test Example 2)

Collagen Fiber Biosynthesis Effect

The cells obtained directly from humans were treated with the extracts obtained in Comparative Examples 1 and 2 and Examples 1 and 2 to measure collagen fiber biosynthesis effects. That is, normal human fibroblasts (ATCC CCL-28) were placed in a culture flask and cultured until 70-80% growth. After treatment with the extracts of Comparative Examples 1 and 2 and Examples 1 and 2, respectively, the collagen fiber biosynthesis measurement kit (BCA protein assay kit, pierce 23225) purchased commercially measured collagen fibers produced It was. At this time, the concentration of each extract added to the culture was 0.01% by weight, 0.10% by weight, 1.00% by weight. The results are shown in Table 2 below.

The kit for measuring collagen fiber biosynthesis, by using an antibody of collagen fibers, binds a chromophore having a specific color to a site bonded after collagen fibers, and measures the degree of color represented by the chromophore at 540 nm (Model name: μ Quant, Bio-Tek instruments, Inc., used the principle of measuring biosynthetic collagen fibers.

Concentration (% by weight) Collagen Fiber Biosynthesis (%) Comparative Example 1 Comparative Example 2 Example 1 Example 2 0.01 108 98 134 156 0.10 120 107 161 173 1.00 129 115 172 181 Control 93 93 93 93 Comparison 62 62 62 62 The control group was not treated with the extracts of Comparative Examples 1 and 2 and Examples 1 and 2, and was not irradiated with ultraviolet rays. The comparative group did not process the extracts of Comparative Examples 1 and 2 and Examples 1 and 2, Investigation.

As shown in Table 2, by combining the silk tree extract in the bark extract, it is possible to obtain a collagen fiber biosynthesis effect that exceeds the effect of collagen fiber biosynthesis of each of the bark extract and silk tree extract. This increased collagen fiber means that wrinkles caused by aging, in particular photoaging, can be prevented using the cosmetic composition of the present invention.

(Test Example 3)

Hyaluronase Inhibitory Effect

Inhibition effects on the activation step of inactive enzymes were measured using hyalurase (Sigma, Type 4). Hyaluronic acid is an enzyme that decomposes hyaluronic acid involved in cell water retention. If hyaluronic acid has an excellent inhibitory activity, it can be judged that hyaluronic acid can increase the stability of hyaluronic acid and increase the water retention capacity of the cell.

10 mg of the extracted sample was dissolved in 100 ml of 0.1 M acetic acid buffer (pH 4.0), placed in a test tube, and 0.1 mg of the enzyme dissolved in 50 ml of acetic acid buffer was added thereto and left at 37 ° C. for 20 minutes. Finally, 200 mg of sodium hyaluronate dissolved in 250 ml of acetic acid buffer was added and left at 37 ° C. for 40 minutes. 100 ml of 0.4 M sodium hydroxide was added to the mixture, followed by cooling, 100 ml of boric acid buffer solution (pH 9.0) was added thereto, followed by incubation at 37 ° C. for 20 minutes, and the absorbance was measured at 585 nm. As a control, an acetic acid buffer solution containing no extract sample solution was used. Inhibitory effect was calculated according to the following formula.

The results are shown in Table 3 below.

Example Hyaluronicase Inhibition Rate (%) Comparative Example 1 70 Comparative Example 2 65 Example 1 84 Example 2 87

As shown in Table 3, by combining the bark extract in the bark extract, the hyaluronase inhibitory effect that exceeds the inhibitory effect of each of the bark extract and the bark extract is obtained.

(Manufacture example 1)

Manufacture of nutritional cream

After dividing and measuring the water phase and the oil phase according to the composition, it was mixed and dissolved separately at 80 ± 2 ℃, the oil phase was mixed in the water phase. Subsequently, the raw material 15 which is a neutralizing agent was added and neutralized, and it cooled to 45 degreeC. After cooling, the raw material 16 was added and cooled to 35 ℃ to prepare a nourishing cream.

Comparative Example 1

A nutritious cream was prepared in the same manner as in Formulation 3 of Preparation Example 1, except that purified water was used instead of the bark extract + silk extract (weight ratio 1: 0.5) of Example 1.

(Test Example 4)

Skin elasticity effect

Thirty healthy women (average 29.5 years old) aged 20 to 35 years old (average 29.5 years old) were divided into 5 groups under the temperature of 20-25 ° C and 60% relative humidity. Formula 2 of Formulation 1, Formula 3 of Preparation Example 1 in Group C, Prescription 4 in Preparation Example 1 in Group D, and skin elasticity enhancing effect of the cosmetics of Comparative Preparation Example 1 in Group E were measured.

After the cosmetics were used for 12 weeks (2 times / day) around the eyes, skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM575, Courage + Khazaka). The measurement results were calculated through the following formulas, and their average values for each test group are shown in Table 4 below.

R5 is a value indicating a net elasticity coefficient, the closer to 1, the better the elasticity.

Experimental group Skin elasticity enhancing effect (△ R5) A group 0.08 B group 0.04 C group 0.14 D group 0.17 E group 0.02

As shown in Table 4, the skin elasticity of Group D (Preparation 4 of Preparation Example 1) incorporating the extract of the bark of the bark skin is 112.5%, compared to Group A (Prescription 1 of Preparation Example 1), B group (Preparation This is an increase of 325% compared to the prescription 2) of Example 1. Therefore, it was found that the cosmetics containing the extract of Albizia julibrissin effectively enhanced skin elasticity.

(Test Example 5)

The creamy sample of Preparation Example 1 was applied to the inner soft skin of the upper arm for an adult male and female (20 males and 20 females). The skin condition (skin irritation, erythema, itching, allergy, etc.) after 24 hours was observed, and once again after 24 hours.

The observation results are calculated as (number of subjects showing positive response / total number of subjects) and are shown in Table 1 below.

sample 24 hours later 48 hours later Cream containing 5% of the bark extract + silk extract (weight ratio 1: 0.5) obtained in Preparation Example 1 0/40 0/40 Cream containing 5% of the bark extract + silk extract (weight ratio 1: 1) obtained in Preparation Example 1 0/40 1/40

As shown in the table, the cream containing 5% of bark extract + silk tree extract, only 1 (48 hours) positive for cream containing bark extract + silk extract (weight ratio 1: 1) Only the rest did not show a positive response. Through this, it can be confirmed that the cosmetic composition containing the mixture according to the present invention is excellent in safety for human skin.

(Manufacture example 2)

Preparation of Flexible Lotion

The aqueous phase and the ethanol phase were separated and weighed according to the following composition, and then mixed and dissolved separately, and the ethanol phase was mixed with the aqueous phase. The additives were then added and mixed. This was filtered using a membrane filter (Samdeok Filtration Co., paper filter) to produce a flexible cosmetic water.

(Manufacture example 3)

Manufacture of nutritional lotion

After dividing and measuring the water phase and the oil phase according to the composition, it was mixed and dissolved separately at 80 ± 2 ℃, the oil phase was mixed in the water phase. Subsequently, the raw material 14 which is a neutralizing agent was added and neutralized, and it cooled to 45 degreeC. After cooling, the raw material 15 was added and cooled to 35 ℃ to prepare a nourishing lotion.

(Manufacture example 4)

Preparation of Essence

After dividing and measuring the aqueous phase and oil phase according to the following composition, it was mixed and dissolved separately at 80 ± 2 ℃, and mixed with putting the oil phase in the water phase. The essence was then prepared by adding the fragrance and then cooling to 35 ° C.

As described above, according to the present invention, it is excellent in antioxidant activity, collagen fiber biosynthesis effect as well as skin elasticity enhancing effect and hyaluronase inhibitory effect than the cosmetics containing the extract of pastoral bark, and because it is a natural extract, stability and It is possible to obtain a cosmetic containing a bark extract and silk tree extract having excellent safety.

Claims (1)

An anti-aging cosmetic composition characterized by containing 0.001 to 20% by weight, based on the total weight of the composition, of a mixture of bark extract and silk tree extract in a weight ratio of 1: 0.001 to 1:20.