KR20190066886A - Cosmetic composition with anti oxidant and skin whitening effect which contains ajuga spectabilis extract as a effective ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition having antioxidant and whitening effects, comprising an extract of Ajuga spectabilis as an active ingredient. More specifically, the cosmetic composition is based on the DPPH radical scavenging ability and tyrosinase inhibitory activities that the extract of Ajuga spectabilis has. The cosmetic composition has excellent DPPH radical scavenging ability and tyrosinase inhibitory activities and is simple to produce, and thus is cost-effective.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition having an antioxidant and whitening effect, which contains an extract from a plant of the genus Escherichia coli,

The present invention relates to a cosmetic composition having antioxidative and whitening effects, and more particularly, to a cosmetic composition having excellent DPPH radical scavenging activity and tyrosinase inhibitory activity, containing an overgrown extract.

When human skin reacts to external stimuli such as ultraviolet rays, environmental pollution or stress, melanin is overexpressed and is not released out of the skin. It is transferred to keratinocyte and accumulates in the skin surface layer, Not only cause serious cosmetic problems such as aging, but also promotes skin aging and skin cancer.

Methods for controlling melanin pigment formation include a method of decolorizing melanin pigments by reducing them and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment. Tocopherol, which is used for reducing melanin pigment, Or vitamins, it is known that the decolorizing effect of the melanin pigment is very small. Thus, an inhibitor that inhibits the activity of tyrosinase has been attracting attention.

As a whitening ingredient in the conventional cosmetics field, for example, substances inhibiting tyrosinase enzyme activity such as kojic acid and arbutin, hydroquinone, L-ascorbic acid, And derivatives thereof and various plant extracts have been used. However, the use thereof is limited due to poor stability in the prescription system and coloration, generation of odor, efficacy at a living body level, unclear effects, and safety problems.

On the other hand, antioxidative capacity is a problem in skin health and aging. In the above antioxidant ability, since the action of preventing the oxidation or aging of cells in the body by removing active oxygen is the key, the active oxygen scavenging Ability can be related to skin protection.

From this point of view, research and development on cosmetics having excellent whitening effect and antioxidant ability are underway. However, cosmetics should be free of side effects, be human-friendly, and be safe to use for specific skin, atopic or sensitive skin. Thus, plant natural products are not only safe, but also have various components beneficial to the human body, and thus they are preferred as ingredients that provide moisturizing, whitening, and anti-aging effects. Accordingly, there has been an effort to find extracts which are excellent in safety from various herbal medicines, vegetables, fruits, flowers and the like, which have functions of suppressing skin aging and whitening function, and providing various functions.

Korean Patent Laid-Open No. 2003-0064059 (extract of Momordica japonica), Korean Patent Publication No. 2003-0064059 (extract of rose flower leaf) has been used as a prior art for a cosmetic composition having antioxidant and whitening effect including a natural extract, And the like. In addition, Japanese Patent Laid-Open No. 10-1766233 discloses a method for extracting a composition for preventing neuropathy from a plant-grown extract. Korean Patent Publication No. 2003-0064059 has only anti-aging function, Korean Patent Publication No. 2003-0064059 has only skin whitening function, and one extract has antioxidant, whitening effect, wrinkle improving effect, There is a limitation that it can not show various effects at the same time. In addition, since Patent Literature 10-1766233 relates to a method for preparing luteolin 5-glucoside or a pharmaceutically acceptable salt thereof for preventing neurological diseases from the herbal extract, far.

Therefore, it is urgent to research and develop a cosmetic composition having a whitening effect and antioxidative effect, which can be easily manufactured using raw materials that are more readily available.

Korea Patent Publication No. 2003-0064059 Korea Patent Publication No. 2003-0064059

The present invention provides a cosmetic composition having antioxidative and whitening effects.

In order to solve the above-mentioned problems, the present invention provides a cosmetic composition having antioxidant and whitening effect containing a prolonged extract as another problem to be solved.

The cosmetic composition containing the prolonged extract prepared according to the present invention is characterized by excellent DPPH radical scavenging activity and tyrosinase inhibiting activity.

According to an aspect of the present invention,

Wherein the cosmetic composition has excellent DPPH radical scavenging activity and tyrosinase inhibitory activity. The cosmetic composition according to claim 1, wherein the cosmetic composition is a cosmetic composition.

The cosmetic composition prepared by the present invention is excellent in DPPH radical scavenging activity and tyrosinase inhibiting activity, and is easy to manufacture and economical.

The cosmetic composition of the present invention may be prepared by mixing a cosmetic composition with a moisturizer, an emollient, a surfactant, an organic powder, an ultraviolet absorber, a pH adjuster, an alcohol, a coloring agent, a perfume, a blood circulation accelerator, Various makeup cosmetics such as lotion, lotion, lotion, lotion, shampoo, rinse, etc., soap, manicure, ode, various cosmetics such as foundation cosmetics such as lotion, lotion, Cologne, as well as lotions, emulsions, ointments, sols, gels, powders, sprays, solids and the like.

Figure 1 shows the DPPH radical scavenging ability of the grown extract.
Fig. 2 shows tyrosinase inhibitory activity of the ginseng extract.

Hereinafter, the present invention will be described in more detail with reference to examples. The present invention can be variously modified and can have various embodiments, and the claims can be divided into a case where the contents of the description are narrowed down, a case where the description is expanded, .

The scope of the invention is narrower than the description in the description of the invention. In contrast to the case of the reduced substrate, the extended substrate is deemed to be included in the claim scope rather than the matters described in the detailed description of the invention. This is a case where a technical scope is broadly described. The same description refers to the case where the matters described in the detailed description of the invention are similarly described in the claims.

It should be apparent that the invention is not to be construed as limited in scope.

It is to be understood that the invention is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

The terms first, second, A, B, etc. may be used to describe various elements, but the elements should not be limited by the terms.

The terms are used only for the purpose of distinguishing one component from another.

For example, without departing from the scope of the present invention, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component.

And / or < / RTI > includes any combination of a plurality of related listed items or any of a plurality of related listed items.

It is to be understood that when an element is referred to as being "connected" or "connected" to another element, it may be directly connected or connected to the other element, .

On the other hand, when an element is referred to as being "directly connected" or "directly connected" to another element, it should be understood that there are no other elements in between.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention.

The singular expressions include plural expressions unless the context clearly dictates otherwise.

In the present application, the terms "comprises" or "having" are used to specify that a feature, temperature range, step, time, or combination thereof is described in the specification, and that the presence of one or more other features, Steps, times, or combinations or combinations thereof in any manner whatsoever without departing from the spirit and scope of the invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The present invention relates to a cosmetic composition having an antioxidative, whitening effect containing an overgrown extract.

Ajuga spectabilis belongs to the family Lamiaceae and is a perennial herbaceous plant that grows in the mountains of southern central part of Korea. The growth environment is rich in lateral leaf growth and grows under the tree of a semi-sagittal or bilge. It is about 50cm tall. Leaves are slightly hairy on the surface and back vein. There are irregular sawtooth and hairs on the edge, about 17cm in length and about 9cm in width. The flower is attached to the end of stem or petiole, purple, and runs about 3 cm in length. The fruit runs around August and is round and wrinkled. It is mainly used for ornamental purposes.

Using the above-mentioned hot water and ethanol extract, the cosmetic composition having antioxidative and whitening effect is prepared.

Production Example 1. Preparation of the Extract of Extracts

Extracts were prepared by mixing distilled water and ethanol in proportions, and each extract was fractionated with ethyl acetate (EtOAc) to obtain an EtOAc fraction extract.

1) Extraction of distilled water

(1) Distilled water was added in an amount of 10 times to 2,000 g of the autoclave and heated at 80 to 100 캜 for 3 hours, followed by filtration, and the filtrate was vacuum dried to obtain 439.5 g of a dried product.

② The procedure of ① was vacuum-dried, and the extract was dissolved in 3,900 mL of distilled water and the same amount of EtOAc was added to the reaction mixture. The EtOAc fraction extract and the distilled water fraction were obtained twice.

2) 30% ethanol extract

1) 30 g of 30% ethanol was added to 2,000 g of the grown solution, heated at 80 to 100 ° C for 3 hours, filtered, and the filtrate was vacuum dried to obtain 409.2 g of a dried product.

② The process of vacuum drying in step ①, dissolving the extract in 3,600 mL of distilled water and partitioning by adding the same amount of EtOAc was repeated twice. 17.4 g of EtOAc extract and 317 g of distilled water fraction were obtained.

3) 70% ethanol extract

(1) 10 g of 70% ethanol was added to 2,000 g of the grown solution, heated at 80 to 100 ° C for 3 hours, filtered, and the filtrate was vacuum dried to obtain 436.7 g of a dried product.

② The procedure of ① was vacuum-dried, and the extract was dissolved in 3,800 mL of distilled water. The same amount of EtOAc was added to the mixture to separate the fractions twice. 31.5 g of EtOAc extract and 342.6 g of distilled water fraction were obtained.

4) 95% ethanol extract

(1) 10 g of 95% ethanol was added to 2,000 g of bovine serum, heated at 80 to 100 ° C for 3 hours, filtered, and the filtrate was vacuum-dried to obtain 284.8 g of a dried product.

② The procedure of ① was vacuum-dried, and the extract was dissolved in 2,300 mL of distilled water and the same amount of EtOAc was added to the reaction mixture twice. The EtOAc extract (35.3 g) and distilled water fraction (177.8 g) were obtained.

The list and yield of the extracts obtained as a result of the extraction are summarized in Table 1 below.

Sample number List yield(%) One Extracted freshly distilled water 23.44 2 Growth 30% ethanol extract 25.78 3 Extracted 70% ethanol extract 28.55 4 Growing 95% ethanol extract 22.40 5 Freshly distilled water Extract EtOAc fraction 8.09 6 Freshly grown 30% ethanol extract EtOAc fraction 6.44 7 Freshly grown 30% ethanol extract EtOAc fraction 12.77 8 Freshly grown 30% ethanol extract EtOAc fraction 15.42

Experimental Example 1 Determination of DPPH Radical Scavenging Ability of the Extracts of Overgrown Extracts

The DPPH assay is one of the most common experiments to measure antioxidant efficacy. The principle of DPPH assay is that Diphenylpicrylhydrazyl which is deep violet is reduced to Diphenylpicrylhydrazine by antioxidant and turns into light yellow. It becomes closer to pale yellow with strong antioxidant power of antioxidant. The experimental procedure is as follows.

 Three groups of 0.1 mM DPPH solution (Diphenylpicrylhydrazyl) were prepared. Group 1 was treated with 100 μM ascorbic acid as a positive control. In the other two groups, the hot water extract of Sample No. 1 and the 30% ethanol extract of Sample No. 2 were injected at a dose of 100 μg / mL. As a result, 35% of the hot water extract and 57% of the EtOH extract showed DPPH radical scavenging ability. The results are shown in Fig.

Experimental Example 2 Determination of tyrosinase inhibitory activity of the ginseng extract

The inhibitory effect of tyrosinase production in the cells was measured for Sample Nos. 1 and 2 extracted in the above Production Example.

Fetal bovine serum (FBS) and Dulbecco's Modified Eagle Medium (DMEM) used in the cell experiments were purchased from Gibco (USA), and the cell line used was mouse-derived B16F10 (melanoma cell) purchased from Korean Cell Line Bank.

Cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco) and 1% antibiotic-antimycotic (Gibco) at 37 ° C in a 5% CO2 incubator and subcultured at 3-4 days intervals. The cultured B16F10 cells were detached with 0.05% trypsin-EDTA, inoculated in the same number (1.0X105 cells / well) to a 24-well plate, and then replaced with serum-free medium for 16 hours. After additional incubation for 6 hours, α-MSH (100 nM) and test drug were treated and cultured for 3 days. After 3 days of incubation, the cells were washed twice with cold PBS (phosphate buffered saline), and then treated with RIPA buffer (50 nM Tris-HCl, 150 nM NaCl, 2% NP-40, 0.5% SDS, pH 7.4), and the cells were disrupted. The recovered pulverized solution was centrifuged at 4 ° C for 30 minutes at a rate of 13,000 rpm to recover only the supernatant containing the protein. The recovered supernatant was quantified by bradford assay. 198 200 of protein at 200 / / ml and 2 2 of 2 ㎎ / ㎖ L-DOPA, and incubated in a 37 ° C CO2 incubator for 1 hour. The absorbance was then measured at 405 nm. Arbutin was used as a control, and the formula was as follows.

Intracellular tyrosinase activity rate (%) = (sample group / control group) * 100

As a result, tyrosinase inhibitory activity was 31% for hot water extract and 43% for EtOH extract. The results are shown in Fig.

As shown in FIGS. 1 and 2, the cosmetic composition containing the herbal extract has excellent antioxidative and whitening efficacy because it has excellent DPPH radical scavenging activity and tyrosinase inhibitory activity. Therefore, the cosmetic composition containing cream, milk powder, lotion, Lotions, emulsions, and cosmetics such as various cosmetics, foundation, lipstick, lotion, white powder, hair lotion, various hair cosmetics such as shampoo and rinse, soap, manicure oil, Ointment, sol, gel, powder, spray, solid, and the like.

Claims (3)

A cosmetic composition containing an extruded hot water extract A cosmetic composition containing an overgrown ethanol extract 3. The method according to claim 1 or 2,
Wherein the cosmetic composition is excellent in DPPH radical scavenging activity and tyrosinase inhibiting activity

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