KR20090011226A - Composition of skin external application containing green tea flower essential oil - Google Patents

Abstract

A composition for skin external application containing green tea flower essential oil is provided to protect skin cell, to improve wrinkle, to suppress deterioration of skin elasticity and pigmentation and to remove active oxygen species. A composition for skin external application comprises green tea flower essential oil to active ingredient. The green tea flower essential oil is a green tea fragrance component obtained through a steam distillation after extracting a solvent of the green tea flower. The green tea flower fragrance component contains 4,8-dimethyl-1,3(E), 7-nonatriene and Syringa vulgaris alcohol.

Description

Composition for skin external application containing green tea flower essential oils

The present invention green tea flower ( Camellia sinensis var . sinenis cv. Shuixian and Maoxie) A skin external preparation composition containing essential oils as an active ingredient, more specifically, reactive oxygen species generated by ultraviolet rays by containing green tea flower essential oils containing green tea flower fragrance ingredients. The present invention relates to a composition capable of preventing cellular damage by removing species, ROS), reducing inflammation of the skin, delaying aging, inhibiting wrinkle formation, and preventing skin elasticity.

The skin is the body's primary barrier, protecting body organs from changes in temperature and humidity, ultraviolet rays, and pollutants. However, internally, as hormones age, the secretion of various hormones that regulate metabolism decreases, and the function and activity of immune cells decreases, resulting in decreased biosynthesis of immune proteins and bioconstituent proteins necessary for living organisms. For example, physical and chemical irritation and stress caused by an increase in ultraviolet rays, free radicals and free radicals due to environmental pollution such as ozone layer destruction, weaken the normal function of the skin, promote aging and pigmentation. In order to prevent this phenomenon and maintain a healthier and more beautiful skin, by using physiologically active substances obtained from various known animals, plants, microorganisms, etc. in cosmetics, it maintains the unique function of the skin and activates skin cells. Efforts have been made to effectively inhibit aging.

Green Tea Tree ( Camellia) sinensis var . sinenis cv. The leaves of Shuixian and Maoxie have been interested for a long time and have used green tea extract and each component importantly, and the catechins, which are a component of green tea extract and green tea, have many effects on skin aging. However, green tea flowers are not known for their useful value and do not help the production of green tea leaves. Recently, green tea flowers have a distinctive fresh scent, and the value as a flavoring plant has attracted attention, and a pharmaceutical composition that reproduces the scent of green tea flowers has begun to be developed (Korean Patent No. 10-0645879: Flavor Composition Reproducing Scent of Green Tea Flower). {Perfume composition for expressing the fragrance of Green tea flower}). However, it is still unknown about the skin efficacy of tea flower essential oil (Tea flower essential oil), such as improving the wrinkles of the skin, improve elasticity, strengthen the skin moisturizing control of micro-inflammation.

Thus, the present inventors, through the study of ingredients that protect the skin from external harmful environment or ultraviolet rays and delay skin aging, when the green tea flower essential oil contains the essential oil (精油) as an active ingredient in the skin external preparation composition is produced by ultraviolet rays Natural moisturizing while eliminating reactive oxygen species (ROS) and controlling fine inflammation produced by external stimuli, protecting skin cells, delaying skin aging, suppressing wrinkle formation and skin elasticity and promoting differentiation of keratinocytes It has been found to promote factor production, enhancing skin moisturization and reducing pigmentation.

After all, it is an object of the present invention to provide a skin external preparation composition containing green tea flower essential oil to inhibit skin aging and enhance skin moisturizing as well as to improve the condition of the skin as a whole.

In order to achieve the above object, the present invention provides a skin external preparation composition containing the green tea flower essential oil (精油) as an active ingredient.

In the present invention, the green tea flower essential oil (essential oil) is a fragrance component obtained by steam distillation after extracting the green tea flower, and the green tea flower fragrance component is 4,8-dimethyl-1,3 (E), 7 Nonatriene (E-4,8-dimethyl-1,3,7-nonatriene) and lilac alcohol (lilac alcohol) is the main ingredient.

In addition, the tea flower essential oil (精油) is contained 0.0001 to 30.0% by weight based on the total weight of the composition.

In the present invention, the composition may be characterized in that to remove the reactive oxygen species (ROS).

In another aspect, the present invention provides a skin external composition for preventing skin aging containing green tea flower essential oil (精油) as an active ingredient.

In addition, the external composition for skin containing the green tea flower essential oil according to the present invention has the effect of preventing skin aging, preventing and improving skin inflammation, preventing and improving skin wrinkle formation, improving skin elasticity, skin whitening, and skin moisturizing.

The external composition for skin containing the essential oil of green tea flowers of the present invention protects skin cells, improves wrinkles, suppresses skin elasticity, pigmentation, etc., and has excellent effects of delaying skin aging and moisturizing the skin. The strengthening effect is excellent.

The external preparation composition for skin of the present invention contains green tea flower essential oil (精油) as an active ingredient.

Green tea flower essential oil used in the present invention (精油) is a green tea flower extract containing the fragrance component of the green tea flower essential oil, the oil component obtained by steam distillation after extracting the green tea flower 4,8- dimethyl Essential oils that provide a distinctive flavor and potency, containing -1,3 (E), 7-nonatriene and lilac alcohol精油) is called.

The green tea flower essential oil (精油) is acetophenone (acetophenone), alpha-methylbenzyl alcohol (α-methylbenzyl alcohol), 2-phenylethyl alcohol (2-phenylethyl alcohol), which are included in the essential oils such as rose essential oil or geranium in addition to the two specific components alcohol) and benzyl alcohol.

The method for obtaining green tea flower essential oil is as follows: However, the green tea flower essential oil in the present invention is not particularly limited in its extraction method for the purpose of using green tea flowers. It can change depending on the purpose.

720 g of green tea flowers in Jeju Island Seogwang Tea Garden were divided into three 1 liter triangle flasks, and 1.5 liters of ethyl ether (99.0% purity, Fisher Scientific) were poured into the flowers so that they were completely submerged and soaked for 4 hours and 15 minutes. . Deposits the ad room Tech then filtered through a No. 2 filter paper (Advantec No.2, diameter 185mm) flowers was discarded, extract with anhydrous sodium sulfate (Na ₂ SO _4, Sodium Sulfate, purity 99.7%, Sigma Aldrich) 100g of water It removed and filtered using Advantech No. 2 filter paper again. Then, the solvent ethyl ether was volatilized in a water bath at 38 ° C. to obtain a residue. 100 ml of distilled water was added to the residue, followed by steam distillation under reduced pressure while maintaining the temperature at 30 占 폚 to obtain 13.2 mg of a concentrated concentrate. The fragrance concentrate was placed in an uncontaminated glass jar for analysis, closed and sealed and stored in the refrigerator.

Ethyl ether, solvent, was volatilized to give a residue. 100 ml of distilled water was added to the residue, followed by steam distillation under reduced pressure while maintaining the temperature at 30 占 폚 to obtain 13.2 mg of a concentrated concentrate.

In order to obtain the desired effect in the present invention, the green tea flower essential oil (精油) is contained in 0.0001 to 30.0% by weight based on the total weight of the composition. If the content of the component is less than 0.0001% by weight it is difficult to expect to act as an active ingredient, if the content exceeds 30.0% by weight may cause problems in formulation stability.

According to the present invention, the external composition for skin containing the essential oil of green tea flower has an effect of improving the condition of the skin by removing the reactive oxygen species (ROS) generated by ultraviolet rays and having a detoxifying effect.

In addition, the external composition for skin containing the green tea flower essential oil according to the present invention has the effect of preventing skin aging, preventing and improving skin inflammation, preventing and improving skin wrinkle formation, improving skin elasticity, skin whitening, and skin moisturizing.

The external preparation composition for skin according to the present invention is not particularly limited in the formulation, for example, a cosmetic having a formulation of softening water, nourishing cosmetics, massage cream, nourishing cream, pack, gel, essence or skin adhesive cosmetic, Or in transdermal dosage forms such as lotions, ointments, gels, creams, patches or sprays.

In addition, in each formulation of the external preparation composition for skin, other components than the essential ingredients described above may be selected and blended by those skilled in the art without difficulty according to the formulation or purpose of use of the external preparation for skin.

Hereinafter, the present invention will be described in more detail with reference to test examples and examples.

Reference Example 1 Preparation of Green Tea Flower Essential Oil and Identification of Ingredients

To extract essential oils, 720g of green tea flowers in Seogwang Dawon, Jeju Island, were divided into three 1-liter Erlenmeyer flasks and ethyl ether (99.9% purity) to immerse the flowers completely. Scientific) 1.5 liters were poured and soaked for 4 hours and 15 minutes. Deposits the ad room Tech then filtered through a No. 2 filter paper (Advantec No.2, diameter 185mm) flowers was discarded, extract with anhydrous sodium sulfate (Na ₂ SO _4, Sodium Sulfate, purity 99.7%, Sigma Aldrich) 100g of water After removing, the resultant was filtered again using Advantech No. 2 filter paper. Then, ethyl ether as a solvent was evaporated in a water bath at 38 ° C., and vacuum distillation was carried out again to obtain 13.2 mg of a concentrated concentrate. The fragrance concentrate was placed in an uncontaminated glass jar for analysis, closed and sealed and stored in the refrigerator.

Reference Example 2 Preparation of Green Tea Leaf Extract

1 kg of green tea leaves obtained from Seogwang Dawon in Jeju Island were extracted using 5L of hexane or similar organic solvent, and the organic solvent layer was separated and removed. The above process was repeated three more times to obtain green tea leaves from which the oil was completely removed. 5 liters of organic solvent (ethanol, methanol, butanol, ether, ethyl acetate, chloroform, etc.) or organic solvent containing water and water was added to the green tea leaves, and the mixture was extracted at room temperature three times and left at 15 ° C. for one day. Was deposited. The above process was repeated twice to obtain an extract, and the extract was filtered through Advantec No. 2 filter paper (Advantec No. 2, diameter 185 mm), and the filtrate was concentrated in a vacuum concentrator maintaining a temperature of 40 ° C. or lower. 89 g of dried material was obtained.

Reference Example 3 Preparation of Green Tea Flower Extract

To extract the green tea flower extract, 3L of ethanol or a similar organic solvent was poured after 1Kg of green tea flowers blooming in Seogwang Dawon, Jeju Island, and deposited for 15 hours. The precipitate was filtered through Advantec No. 2 filter paper (Advantec No. 2, diameter 185 mm), and the flowers were discarded. The filtrate was concentrated in a vacuum concentrator maintaining a temperature of 40 ° C. or lower to obtain 12.5 g of dried matter.

Analysis Example 1

The green tea flower essential oil obtained in Reference Example 1 was injected into the GC inlet, and GC-MS analysis was performed. GC-MS analysis conditions are as follows.

Analysis condition

. Analyzer: HP 6890 II GC. Detector: HP 5973 MSD

. Column: PEG-20M (60mX0.25mmX0.25um). Carrier Gas: He

. Injection Temperature: 250 ° C. Detector Temperature: 280 ℃

. Oven Temperature: 70 ° C-220 ° C (3 ° C / min). Ionization Voltage: 70eV

. Injection Volume: 1ml. Split Ratio: 1:40

As a result, the fragrance components of the green tea flower essential oil obtained by the steam distillation method are shown in Table 1 below.

Spices people content(%) 4,8-Dimethyl-1,3 (E), 7-nonatrien 0.008 Lilac Alcohol 0.048 Acetophenone 31.101 Alpha-Methylbenzyl Alcohol 23.600 2-phenylethyl alcohol 10.150 Linalule 3.530 Geraniol 1.941 Linalul oxide 2.250 Methyl Salicylate 0.110 Indole 0.050 vanillin 0.040 Etc 27.172 Total content 100.000

As shown in Table 1, green tea flower essential oil is a unique ingredient, 4,8-dimethyl-1,3 (E), 7-nonatriene (E-4,8-dimethyl-1,3,7). It contains acetophenone, alpha-methylbenzyl alcohol and 2-phenylethyl alcohol, which contain nonatriene) and lilac alcohol, and are distinguished from other commonly used rose essential oils such as rose essential oil and geranium essential oil. . These major components make up 64.91% of the total.

Test Example 1 Measurement of Inhibition Effect of Reactive Oxygen Species (ROS) Formation by UV Light

The cell line used in the experiment was based on the results of Dr. Human kerationocyte HaCaT cell line supplied from Fusenig at 37 ° C and 5% CO ₂ in a 96-hole plate incubator for fluorescence measurement at 2 x 10 ⁴ concentrations per well Incubated for 1 day under conditions. For comparison with the effect of the green tea flower essential oil (精油) obtained from Reference Example 1, the green tea leaf simple extract of Reference Example 2 and the green tea flower simple extract of Reference Example 3 were treated for 24 hours as shown in Table 2 below.

100 μl of DCFH-DA (2 ', 7-dichlorodihydro-fluorescein diacetate, Molecular Probes, Inc) prepared at 20 μM in HEPES-buffered control salt solution (HCSS) was added and incubated for 20 minutes at 37 ° C and 5% CO _2. Washed with HCSS. After adding 100 µl of HCSS containing the same sample, which was pretreated for 24 hours, the fluorescence intensity of DCF (dichlorofluorescein) oxidized with reactive oxygen species (ROS) was measured with a fluorescent plate reader (Ex = 485 nm, Em = 530 nm). UVB (30 mJ / cm ² ) was treated and the fluorescence intensity immediately after treatment and up to 3 hours was measured with a fluorescence plate reader (Ex = 485 nm, Em = 530 nm). The results are shown in Table 2 below as a comparison of the non-treated control (ultraviolet control) that induced ROS generation with ultraviolet rays as 100.

Concentration % ROS generation after 3 hours UV control - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 56 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 75 Green tea flower simple extract treatment group (Reference Example 3) 0.1 ppm 68

From Table 2, it was confirmed that the green tea flower essential oil (精油) of the present invention effectively reduces the generation of reactive oxygen species (ROS) by ultraviolet rays. The effect is superior to the simple extract of green tea leaf or simple extract of green tea flower Therefore, the green tea flower essential oil of the present invention can effectively protect against oxidative stress and harmful environment such as air pollution, yellow sand, ozone as well as ultraviolet rays. It can be seen that it can act as a detoxifying effect to protect the cells from.

Test Example 2 Determination of Biosynthesis Inhibition Effect of Type I Collagenase (MMP-1) by Ultraviolet Rays

Human fibroblasts were incubated in a 48-hole flat plate incubator at 10 ⁴ concentrations, irradiated with UV A at 15 J / cm ² after 24 hours, and then the green tea leaf simple extract for comparison with the essential oil of reference flower 1 And the green tea flower simple extract was replaced with a medium containing as shown in Table 3. The supernatant was harvested on day 2 of culture and the amount of type I collagenase produced was quantified using ELISA (AP biotech RPN2610) method. The results are shown in Table 3 comparing the UV control group to 100.

Concentration Type I collagenase biosynthesis (%) UV control - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 60 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 85 Green tea flower simple extract treatment group (Reference Example 3) 0.1 ppm 78

From Table 2, it can be seen that the green tea flower essential oil of the present invention effectively reduces the biosynthesis of type I collagenase by ultraviolet rays and has a greater effect when compared to the green tea leaf extract and the green tea flower extract that are simply extracted. . Therefore, it can be seen that collagen generated normally in the skin due to the expression of collagenase can be prevented or suppressed from wrinkles generated.

Experiment 3 Measurement of Biosynthesis Inhibition Effect of Tumor Necrosis Factor Alpha by Ultraviolet Rays

Human keratinocytes were cultured in a 12-hole plate incubator at a concentration of 10 ⁵ , and after 24 hours, irradiated with ultraviolet B at 30 mJ / cm ² , the green tea leaf was simple for comparison with the essential oil of the green tea flower of Reference Example 1. Extract and green tea flower simple extract was replaced with a medium containing as shown in Table 4. Cells were harvested 6-24 hours after culture (harvest) to quantify the amount of tumor necrosis factor alpha (TNF-α) produced using the ELISA (Pharmingen 555212) method. Tumor necrosis factor alpha is an indicator for measuring the microinflammatory response of skin and is known to mediate the reduction of skin matrix material. The results are shown in Table 4 below with a UV control of 100.

Concentration TNF-α biosynthesis (%) UV control - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 57 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 75 Green tea flower simple extract treatment group (Reference Example 3) 0.1 ppm 78

From Table 4, it was confirmed that the green tea flower essential oil of the present invention effectively reduces the biosynthesis of tumor necrosis factor alpha by ultraviolet rays, the effect is greater when compared to the green tea leaf simple extract and green tea flower simple extract. Able to know. Therefore, it can be seen that it is possible to effectively prevent or improve skin micro-inflammation that may occur due to ultraviolet rays, reduction of skin matrix material due to such skin micro-inflammatory, decrease in elasticity, wrinkle formation and the like.

Test Example 4 Measurement of Collagen Biosynthesis Efficacy of Skin Cells

After culturing human fibroblasts in a 48-hole flat plate incubator at a concentration of 10 ⁴ , a medium containing the green tea leaf simple extract and the green tea flower simple extract as shown in Table 5 for the comparison of the efficacy with the green tea flower essential oil of Reference Example 1 Replaced with. After 24 hours of culture, the supernatant was harvested and the amount of procollagen produced was quantified using the ELISA (Takara MK101) method. The results are shown in Table 5 below, with the control as 100.

Concentration Collagen Biosynthesis (%) Untreated control - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 135 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 98 Green tea flower simple extract treatment group (Reference Example 3) 0.1 ppm 104

From Table 5, it was confirmed that the green tea flower essential oil (精油) of the present invention effectively increases the biosynthesis of type I collagen. Therefore, it can be seen that the normal collagen production of new collagen can be prevented or suppressed in the skin in which collagen production decreases with aging.

Test Example 5 Measurement of Tropoelastin and Fibrillin Production in Human Skin Fibroblasts

After culturing human fibroblasts in a 48-hole flat plate incubator at a concentration of 10 ⁴ , a medium containing the green tea leaf simple extract and the green tea flower simple extract as shown in Table 6 for the comparison of the efficacy with the green tea flower essential oil of Reference Example 1 Replaced with. After 24 hours of culture, the supernatant was harvested and Western blot was used to quantify tropoelastin and fibrin. The results are shown in Table 6 comparing the control to 100 after image analysis.

Concentration Tropoelastin Production (%) Fibrillin production (%) Untreated control - 100 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 125 135 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 100 98 Green tea flower simple extract treatment group (Reference Example 3) 0.1 ppm 101 104

From Table 6, it was confirmed that the green tea flower essential oil (精油) of the present invention effectively increases tropoelastin and fibrin biosynthesis, two proteins important for skin elasticity. Therefore, it can be seen that the elastic fibers are weakened according to aging to promote the production of new elastic fiber constituent proteins normally, thereby maintaining skin elasticity and strengthening the reduced elasticity.

Test Example 6 Differentiation Induction Test of Human Keratinocytes

In order to confirm the skin barrier function and skin moisturizing ability, the human keratinocytes cultured in the primary culture by the absorbance test were attached to the bottom of the culture flask, and then compared to the essential oils of tea flower essential oil of Reference Example 1 For the green tea leaf simple extract and green tea flower simple extract was added to the culture solution as shown in Table 7, the cells were incubated for 5 days until the growth of 70 ~ 80% of the floor area. The cells were harvested and washed with PBS (phosphate buffered saline), followed by 10 mM Tris-HCl (Tris-HCl) containing 2% Sodium Dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT). , pH 7.4) 1ml was added to perform sonication for 3 minutes, and then boiled for 10 minutes. The precipitate was separated by centrifugation at 1200 rpm for 30 minutes and suspended in 1 ml of PBS to measure absorbance at 340 nm.

Separately, a portion of the solution after the sonication was taken to measure the protein content, which was used as a reference when evaluating the degree of cell differentiation. Low calcium (0.03mM) treated group and high calcium (1.2 mM) treated group as a negative / positive control, respectively, and the test results performed by adding the test substance to the low calcium concentration is shown in Table 7 below.

Concentration Differentiation capacity in keratinocytes (%) Control Low Ca ^{2 +} (0.03 mM) 100 Hign Ca ^{2 +} (1.2 mM) 210 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 124 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 98 Green tea flower simple extract treatment group (Reference Example 3) 0.1 ppm 108

As shown in Table 7, the result of comparing the cell differentiation promoting effect by measuring the amount of CE (Cornified Envelop) produced during keratinocyte differentiation Green tea flower essential oil (精油) according to the present invention can be seen to promote differentiation in keratinocytes. Therefore, it can be seen that it enhances the barrier function of the skin and promotes skin moisturizing ability.

Test Example 7 Measurement of Filaggrin Expression by RT-PCR Analysis

The cell line used in the experiment was based on the results of Dr. Human kerationocyte HaCaT cell line distributed from Fusenig, which were dispensed into 1 × 10 ⁵ cells in 60 mm dishes and incubated for 1 day, followed by comparison with the essential oil of green tea flower of Reference Example 1 Green tea leaf simple extract and green tea flower simple extract was treated as shown in Table 8 and then incubated for 24 hours.

Total RNA was extracted from the cells cultured and tested by the above method using Trizol (Trizol, Gibco Laboratories, USA), and then the method provided by one step RNA PCR kit (AMV) (Takara Bio Inc., Japan). RT-PCR (reverse transcriptional polymerase chain reaction) analysis was performed. In this case, primers for filaggrin (Bionia, Korea) were used as primers, and beta-actin (β-actin) was used as an internal control to compare expression levels. After the image analysis, a comparison of the control group to 100 is shown in Table 8 below.

Concentration Filla Green Production (%) Untreated control - 100 Green tea flower essential oil treatment group (Reference Example 1) 0.1 ppm 180 Green tea leaf simple extract treatment group (Reference Example 2) 0.1 ppm 112 Green tea flower simple extract treatment group (Reference Example 3) 0.1 ppm 109

As shown in Table 8, it can be seen that the green tea flower essential oil (精油) according to the present invention promotes the expression of filaggrin in keratinocytes. Therefore, it can be seen that it promotes the generation of natural moisturizing factor of the skin and strengthens the skin moisturizing ability.

Test Example 8 Measurement of Melanin Inhibitory Effect Using Pigment Cells

C57BL / 6 mouse-derived pigment cells (Mel-Ab cells) in a 24-hole plate incubator at a concentration of 10 ⁵ and cultured the cells, and the second day from the hydroquinone, green tea flower essential oil of Reference Example 1 (精油) Was added as a test substance and incubated. At this time, the hydroquinone used is a component that suppresses the formation of melanin pigment, a very strong component of the decolorizing effect, the group treated with the hydroquinone was used as a positive control group. Then, the culture medium was removed, washed with PBS, the cells were dissolved with 1N sodium hydroxide, the absorbance was measured at 400 nm, and the melanin production inhibition rate was calculated according to Equation 1 below, and the results are shown in Table 9 below. (Dooley's method).

Concentration Melanin production inhibition rate (%) Hydroquinone control 5 μM 56.4 Green Tea Flower Essential Oil Treatment Group 0.1 ppm 34.5 Green Tea Flower Essential Oil Treatment Group 1 ppm 51.6

As shown in Table 9, the green tea flower essential oil (精油) of the present invention was confirmed to show an excellent melanin inhibition rate when compared with hydroquinone. Through this, it can be seen that the pigmentation of the skin can be suppressed, so that the effect on the skin whitening was confirmed.

Test Example 9 Wrinkle Improvement Effect Measurement

In order to confirm the effect of improving wrinkles in humans by the composition of the present invention, Example 1 was prepared according to the formulation of the external preparation as a nutritional cream formulation. The unit of content ratio of the following compounding component is weight%.

Ingredient Preparation Example 1 Comparative Example 1 Purified water To 100 To 100 Green Tea Flower Essential Oil (Reference Example 1) 0.1 - Vegetable Cured Oil 1.50 1.50 Stearic acid 0.60 0.60 Glycerol Stearate 1.00 1.00 Stearyl alcohol 2.00 2.00 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.00 1.00 Arakidil Behenyl Alcohol & Arakidil Glucoside 1.00 1.00 Cetylaryl Alcohol & Cetearyl Glucoside 2.00 2.00 PEG-100 Stearate & Glycerolate & Propylene Glycol 1.50 1.50 Caprylic / Carric Triglycerides 11.00 11.00 Cyclomedicon 6.00 6.00 Preservative, incense Quantity Quantity Triethanol amine 0.1 0.1

In order to confirm the wrinkle improvement effect of Comparative Example and Preparation Example 1 of Table 10 was evaluated as follows. Eighty healthy women in their thirties were divided into two groups of 20 people for each of the two groups of Comparative Example 1 and Preparation Example 1, and the nourishing cream was applied to the face once a day for 8 weeks. The condition was measured by a skin meter (visiometer, SV600, Courage + Khazaka electronic GmbH, Germany) for image analysis. The results are shown in Table 11 below. The values in Table 11 below represent the average of each parameter value after 8 weeks of application minus the parameter value before application.

Clinical results after 8 weeks of use R1 R2 R3 R4 R5 Comparative Example 1 0.27 0.26 0.21 0.03 0.03 Preparation Example 1 -0.22 -0.21 -0.12 -0.04 -0.03

R1: difference between the highest and lowest of the wrinkle contour

R2: Divide the wrinkle contours by 5 spaces, and then average the R1 values

R3: The highest value among the R1 values divided by five

R4: The mean value of the baseline of the fold contour minus the top and valley values of each angle

R5: Difference value of the baseline of the wrinkle contour line minus each wrinkle outline

As shown in Table 11, the external preparation composition of Preparation Example 1 was found to have a very good skin wrinkle improvement effect. Preparation Example 1 containing the green tea flower essential oil (精油) of the present invention can be seen that there is a wrinkle improvement effect compared to the group to which Comparative Example 1 was applied.

Test Example 10 Measurement of Skin Elasticity Improvement Effect

In order to confirm the effect of improving skin elasticity in humans by the composition of the present invention, Example 1 and Comparative Example 1 of Test Example 9 were evaluated as follows.

40 healthy women in their 30s were divided into two groups of 20 people for the two groups of Comparative Example 1 and Example 1, and the external preparation was applied twice a day for 8 weeks on the face of nutrition cream at a temperature of 24-26 ° C and 75% humidity. After application, skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany). The results are shown in Table 12 below. The results in Table 12 are described as ΔR8 (R8 (left) -R8 (right)) values of Cutometer SEM 575, where R8 values represent the nature of skin viscoelasticity.

Clinical results after 8 weeks of use Skin elasticity effect Comparative Example 1 0.10 Preparation Example 1 0.43

As shown in Table 12, Preparation Example 1 containing the green tea flower essential oil (精油) of the present invention can be seen that the skin elasticity is further increased compared to the group to which Comparative Example 1 was applied.

Separately, at the end of the test, the subjects were asked to fill out a questionnaire, which was evaluated mechanically and subjectively. The results are shown in Table 13 below.

Survey results after 8 weeks of use Respondents (Personal) Very good Good usually Inadequate Comparative Example 1 2 5 5 8 Preparation Example 1 6 9 5 0

As shown in Table 13, it can be confirmed through the survey that the skin elasticity is improved when the Preparation Example 1, the composition according to the present invention is applied.

Test Example 11 Measurement of Skin Moisturizing Improvement

In order to confirm the effect of improving skin moisturization in humans by the composition of the present invention, the following Preparation Example 1 and Comparative Example 1 of Test Example 9 were evaluated.

40 healthy women in their 30s were divided into two groups of 20 people for each of the two groups of Comparative Example 1 and Example 1, and the external preparation was applied twice a day for four weeks on the face of nutrition cream twice a day at a temperature of 24-26 ° C and a humidity of 75%. Constant temperature and constant humidity conditions (24 ° C, 40% relative humidity) after starting the application, after 1 week, 2 weeks, 4 weeks after application, and 2 weeks after the application was stopped (total 6 weeks). ), Skin moisture was measured with a conniometer. The results are shown in Table 14 below by displaying the percentage increase of the measured value after treatment for a period of time based on the Koniometer value measured immediately before the start of the test.

Clinical results Moisture Growth Rate (%) 1 week past 2 weeks past 4 weeks past After 6 weeks Comparative Example 1 30 34 34 15 Preparation Example 1 34 41 42 33

As shown in Table 14, Example 1 containing the green tea flower essential oil (精油) of the present invention was confirmed that the skin moisture retention amount was further increased compared to the group to which the comparative example was applied. Also, two weeks after the application of the test substance was stopped, the value after 6 weeks after the measurement of the skin moisture was similar to that after 1 to 2 weeks. It can be seen that the water retention of the skin is maintained continuously. Therefore, it can be seen that the tea flower essential oil of the present invention enhances skin moisturizing.

Hereinafter, although the formulation example of the skin external preparation composition containing the green tea flower essential oil by this invention is demonstrated more concretely based on said test example, this invention is not limited only to these formulation examples.

Formulation Example 1 Flexible Cosmetic (Skin Lotion)

Ingredient weight% Purified water To 100 Green Tea Flower Essential Oil 0.1 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, flavoring Quantity

Formulation Example 2 Nutritious Longevity (Milk Lotion)

Ingredient weight% Purified water To 100 Green Tea Flower Essential Oil 0.1 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

Formulation Example 3 Nutrition Cream

Ingredient weight% Purified water To 100 Green Tea Flower Essential Oil 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / Capric Triglycerides 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

Formulation Example 4 Massage Cream

Ingredient weight% Purified water To 100 Green Tea Flower Essential Oil 0.1 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / Capric Triglycerides 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, flavoring Quantity

[Formulation Example 5] Pack

Ingredient weight% Purified water To 100 Green Tea Flower Essential Oil 0.1 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Fiji-12 nonylphenyl ether 0.3

Claims (12)

A skin external preparation composition containing green tea flower essential oil as an active ingredient. The skin external composition according to claim 1, wherein the green tea flower essential oil is a green tea flower fragrance ingredient obtained by steam distillation after solvent extraction of the green tea flower. According to claim 2, wherein the green tea flower odor component is 4,8-dimethyl-1,3 (E), 7-nonatriene (E-4,8-dimethyl-1,3,7-nonatriene) and lilac An external preparation composition for skin, comprising alcohol (lilac alcohol). The skin external composition according to any one of claims 1 to 3, wherein the green tea flower essential oil contains 0.0001 to 30.0% by weight based on the total weight of the composition. The composition for applying the external of the skin according to claim 1, wherein the composition removes reactive oxygen species (ROS). A skin external preparation composition for preventing skin aging containing green tea flower essential oil as an active ingredient. A skin external preparation composition for preventing and improving skin inflammation containing green tea flower essential oil as an active ingredient. A skin external preparation composition for preventing and improving skin wrinkle formation containing green tea flower essential oil as an active ingredient. A skin external preparation composition for improving skin elasticity containing green tea flower essential oil as an active ingredient. A skin external preparation composition for skin whitening containing green tea flower essential oil as an active ingredient. Skin external preparation composition containing green tea flower essential oil as an active ingredient. The skin external composition according to any one of claims 6 to 11, wherein the green tea flower essential oil is a green tea flower fragrance ingredient obtained through steam distillation after solvent extraction of the green tea flower.