KR20100109619A - Cosmetic composition containing rumex crispus and pinus strobus bark extract for whitening - Google Patents

Abstract

PURPOSE: A cosmetic composition for skin whitening containing Pinus Strobus bark extract and Rumex Crispus L. extract is provided to suppress SCF(stem cell factor) expression and tyrosinase activity. CONSTITUTION: A cosmetic composition for skin whitening contains Pinus Strobus bark extract and Rumex Crispus L. extract as an active ingredient. The extract contains 0.001-10.0 weight% of each based on total weight. A cosmetic composition for suppressing SCF expression contains Rumex Crispus L. extract as an active ingredient. The composition is used in the form of a skin softener, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, or powder.

Description

Cosmetic composition containing Rumex crispus and Pinus strobus bark extract for whitening}

The present invention relates to a cosmetic composition for skin whitening, more specifically, Rusu extract (Rumex Crispus L.) extract and Strobe pine bark ( Pinus Strobus bark) extract containing the extract as an active ingredient stem cell factor (SCF) Stem Cell Factor), and strobe pine bark relates to a cosmetic composition for skin whitening that exhibits excellent skin whitening effect by inhibiting tyrosinase activity.

The pigments that determine human skin color include melanin, hemoglobin and carotenoids, and the various colors of human skin, hair and eyes are determined by these pigments. The most important pigment that determines the color of the skin is melanin, the skin color is determined by the nature and degree of distribution of melanin.

Melanin is synthesized by melanin forming cells present in the basal cell layer of the epidermis and the unit is Tyrosine. This tyrosine is converted to dopa by tyrosinase enzyme, oxidized and converted back to dopa quinone. The synthesized melanin is transferred to keratinocytes through a melanosomal pouch and then released from keratinocytes to the surface of the skin during 28 days of keratinization. Melanin plays a beneficial role in eliminating free radicals and free radicals naturally occurring in the human skin or preventing the transmission of ultraviolet rays that cause much harm to the skin. However, since tyrosinase is more activated by ultraviolet light, it is well known that the skin turns black when exposed to a lot of ultraviolet light. That is, when the skin is exposed to ultraviolet rays, melanin is synthesized and released in melanocytes, a kind of skin cells, and the skin epidermis becomes black.

In addition, pigmentation may occur if melanin is excessively produced by a factor promoting melanin production and melanin is not completely lost by keratinization.

Therefore, in order to prevent the skin from becoming black, a method of reducing melanogenesis by inhibiting some reactions in the melanogenesis process is the simplest and most common.

In order to prevent pigmentation, many studies on melanin production pathways are being conducted, and raw materials for inhibiting melanin production by acting on each step thereof have been developed. For example, raw materials for inhibiting tyrosinase enzymes related to the synthesis of melanin pigment include arbutin and mulberry extract, and vitamin C derivatives that promote reduction of melanin produced. In addition, there are lactic acid and salicylic acid which promotes exfoliation of melanin in the epidermis to promote exfoliation of keratin, inorganic sunscreens and organic sunscreens that block ultraviolet rays, and hydroquinones that show cytotoxicity to melanocytes. have. Recent research on whitening has progressed to inhibit melanin production by blocking signaling substances such as cytokines that affect melanin synthesis.

However, the raw materials have a problem in cell penetration ability, the inhibitory effect on tyrosinase is insignificant, and some raw materials have the disadvantage of discoloring the skin. In addition, the amount of use is limited due to problems such as safety when formulated in cosmetics, and the development of new whitening raw materials continues to be studied.

Republic of Korea Patent Application No. 1999-0017810 discloses a whitening cosmetic composition comprising the upper limb extract and vitamin C at the same time, Republic of Korea Patent Application No. 1999-0063340 is a retinoid, vitamin C, mulberry extract, licorice derivatives and coenzyme Q10 Disclosed is a skin external preparation composition which is excellent in skin whitening effects containing as an active ingredient.

In addition to these whitening substances, research has been conducted to find whitening active ingredients in natural products, especially plants. Among them, white skin (Japanese Patent Application Laid-Open No. 55-44375, Small 64-26507, Small 64-83009, Pyeong 1-25687 No. 5-139950, Korean Patent Publication Nos. 92-002109, 97-021273, Licorice (Japanese Patent Laid-Open No. 60-214721, No. 60-214728, No. 63-23809, No. 64- 63506, Hei 1-149706, and Korean Patent Nos. 92-002109, 97-025601), Peony (Japanese Patent Laid-Open No. 61-246109 and Korean Laid-Open Patent No. 92-002111), Cinnamon Patent Nos. 63-30403, Hep. 5-139954), Gosam (Japanese Unexamined Patent Publication No. 64-26507 and Korean Unexamined Patent Publication No. 92-002110), Brown root (Japanese Patent Publication No. 60-214727, Small 64-16709 ), Angelica (Japanese Patent Laid-Open No. 56-92211, Hei 4-26610), Mokdanpi (Japanese Laid-Open Patent No. 60-214721, No. 61-50915, No. 61-246109), Banha (Japanese Laid-Open Patent Publication) Pyeong 2-207028, Korea A number of plant extracts and herbal extracts such as Japanese Patent Application Laid-Open No. 96-033442) and Aloe (Japanese Patent Laid-Open No. 52-44375, Hei 2-207030, Hei 5-139950) act on tyrosinase to act as melanin. Although it has been found that the production is suppressed, they also have a lot of problems in the use of more than the effective concentration in cosmetics or pharmaceuticals in terms of safety, discoloration, etc., and still does not show an excellent effect.

In the present invention, by using a traditional herbal medicine that has been used as a folk remedy for a long time, it was intended to develop a cosmetic composition for skin whitening having no skin side effects and excellent skin improvement effect. It was confirmed that the effect of lowering the expression rate was lowered in skin whitening, and as a result of finding a substance with a high inhibition rate of stem cell factor expression, Soru-Ji extract was found to have high inhibition rate of stem cell factor expression. The present invention was completed by confirming that the strobe pine bark extract was mixed with the extract of Sorbus extract to have a synergistic effect on skin whitening and to exhibit an excellent effect on skin safety.

Accordingly, an object of the present invention is to provide a whitening cosmetic composition that can maximize the skin whitening effect by increasing the suppression rate of the stem cell factor expression by mixing the extract of vegetable extracts Soru-Ji and St. It is.

In order to solve the above problems, the present invention may include the following means.

According to one embodiment of the present invention, the whitening cosmetic composition according to the present invention contains the extract of Strobe Pine Bark and Sorbus extract as an active ingredient.

On the other hand, the strobe pine bark extract and the extract is characterized in that it contains 0.001 to 10.0% by weight relative to the total weight of the composition, respectively.

According to another embodiment of the present invention, the cosmetic composition for inhibiting the expression of the stem cell factor according to the present invention contains Soru-Ji extract as an active ingredient.

In addition, the composition is characterized in that it has one formulation selected from the group consisting of softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder do.

Soru-Ji extract of the present invention can be confirmed in the following experimental example to inhibit the stem cell factor (SCF: Stem Cell Factor), but the skin whitening effect per se, and strobe pine bark tyrosinase activity Inhibition of the skin whitening effect can be confirmed, but the suppression rate of the stem cell factor expression was low, it was confirmed that the skin whitening effect can not be maximized. Therefore, the synergistic effect is achieved by simultaneously using the extract of the rubbing extract and the strobe pine bark, thereby providing an effect of helping skin whitening.

Hereinafter, the present invention will be described in detail.

The whitening cosmetic composition according to the present invention relates to a whitening cosmetics to improve the skin color by using the extracts and extracts strobe pine.

Rumex cripus L. used in the present invention is called Yangjae-geun, sol-bim-myeon, Song-gu, etc. It is a perennial grass of the perennial herb that dries up in midsummer and grows again when cool. Young leaves are eaten as herbs and are effective for ringworm, athlete's foot, eczema, etc. Since ancient times, it has been used in skin diseases such as amelioration, scabies and skin cancer. It is called Yang Jae-geun when the roots are broken and dried in the autumn and dried in the sun.

Pinus Strobus used in the present invention is native to North America and distributed throughout the country all over the country can be planted in the sub-central below 500 m above sea level. Strobe pine is an evergreen coniferous tree that grows up to 30m in height and 1m in the previous year. Leaves are 5 in each, linear, 6 ~ 14cm long, pointed at the end, shaped like awl, with serrate, gray-green, and 2 resinous spheres in cross section, external. On the back, there is a white pore ship. In late April, yellow-green flowers bloom, and as a single family flower, female flowers are oval-shaped, and male flowers are cylindrical. Fruits ripen in early October of the following year. Conifer is long cylindrical, 8 ~ 2 cm long, 2.5 cm in diameter, sagging downwards, often bent, and thread fragments spread out in all directions as a branch. Seeds are oval or ovate, 5 ~ 7 cm long, 3 ~ 5 cm wide, brownish brown, but have black spots, and wings are 13 ~ 18 cm long. The stem grows up to 30m in height and 1m in diameter, and the stem is straight, and the bark is flat in greenish brown color, but when it grows old, the stem is deeply divided and the body is greenish brown at first, but disappears. Ingredients included in the neck include finitol, pinosylbin, pinosylbin-methylester, flavonoids chrysin, pinoserline, pinobanksin, and cryptostostrobin. In the literature, thin branches of pine tree are effective for rheumatism, gout, and back pain, and they are used as analgesic baths. As a drug for treating tuberculosis, it is recorded that rink is used for the treatment of infectious wounds caused by Mycobacterium tuberculosis or Staphylococcus aureus.

Soru-Ji extract used as an active ingredient of the cosmetic composition for whitening of the present invention is a cell surface protein of melanocytes (Melanocyte) by inhibiting stem cell factors derived from keratinocytes (Keratinocyte), as can be seen in the following experimental example Inhibits the activity of c-kit protein. This suppresses cell signal transduction in melanocytes, resulting in an excellent skin whitening effect.

Meanwhile, the c-kit protein is a cell surface protein belonging to the receptor tyrosine kinase type III family and is known as a stem cell factor (SCF) receptor. Studies in white spotting and steel mice show that SCF / c-kit function plays an important role in cell maintenance, hematopoiesis, pigmentation and reproduction. In charge. As a result of the interaction between SCF / c-kit, the C-kit protein has dimerization and self-phosphorylated activity. Subsequently, intracellular signal transduction passes through the Ras-Raf-MAP kinase cascade, and finally tyrosinase synthesis is activated by activating the Microphthalmia-Associated Transcription Factor (MITf). To promote melanin production in melanocytes.

SCF / c-kit signal transduction is also related to pigmentation induced by UVB. In fact, stimulation with UVB during cell culture of keratinocytes and melanocytes results in the expression of genes and proteins in SCF and C-kit. You can see the increase. This phenomenon was also found to be significantly increased in the expression of genes and proteins of SCF in the exposed portion compared to the portion not exposed to ultraviolet B when the human epidermis (epidermis) treatment. The same is true for Brown's Guinea pigs, an excellent model for studying melanosite function. That is, when the inhibitory antibody against the C-kit protein was injected into the subepidermal of the experimental animals, it was confirmed that the pigmentation induced by ultraviolet B was completely inhibited. Therefore, it can be seen that signal transduction of SCF / c-kit interaction is ultimately closely related to pigmentation.

The cosmetic composition according to the present invention contains the above-mentioned Soru-Ji extract and Strobe Pine Bark extract as active ingredients. The extract of Soru-Jin inhibits pigmentation induced by UV rays through the inhibition of stem cell factor (SCF), and the extract of Strobe Pine Bark inhibits melanin production and inhibits tyrosinase activity and contains a combination of the two components. The composition was confirmed in the following experimental examples to exhibit an excellent skin whitening effect.

The extract according to the present invention can be extracted by a variety of known methods, but may be prepared as follows.

Thoroughly wash the rust and strobe pine bark with water and dry in the shade. Each dry plant is placed in an extraction container and extracted for a period of time at an appropriate temperature in an appropriate amount of extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used, and preferably ethanol is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine.

The extraction method may be an extraction method such as water bath extraction, reflux cooling extraction, ultrasonic extraction, etc., preferably may be extracted 1 to 5 times as an extraction method of water bath extraction.

The deposition temperature is 30 ~ 70 ℃, more preferably 40 ~ 60 ℃, most preferably maintained at about 50 ℃ is most effective in the prevention of rot, discoloration and odor.

The deposition time is 5 to 20 hours, preferably 10 hours.

After the extract is obtained, solids are removed using a filter paper, the suspension is centrifuged, and the supernatant can be filtered under reduced pressure.

The filtered extract can be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). Fractions are also included in the extract of the present invention, the extract of the present invention can also be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze drying or spray drying, prepared and used using techniques known in the art Or a commercially available one can be purchased and used.

 The extract of the present invention is suitable to include 0.001 ~ 10.0% by weight based on the total cosmetic composition. When the extract of the present invention contains less than 0.001% by weight, the skin whitening effect of the cosmetic composition is insignificant, and when added in excess of 10.0% by weight, the synergistic effect due to the overinjection is not economical, and the product stability is not good. Can affect More preferably, the extract of the present invention preferably contains 0.1 to 5.0% by weight.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions, in addition to the extracts Sorbus extract and strobe pine bark as active ingredients, for example, such as antioxidants, stabilizers, solubilizers, pigments and fragrances Phosphorus adjuvants, and carriers.

The cosmetic composition for skin whitening of the present invention, in addition to the sorbus extract and the strobe pine extract, does not impair the whitening effect of the present invention, and preferably other whitening components that may give a synergistic effect to the whitening effect. It may also contain such. In addition, it may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powders It may be formulated as a foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, the carrier components include animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc or zinc oxide. Can be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminium hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of a spray, additionally chlorofluorohydrocarbons. Propellant, such as propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Aliphatic esters of 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycols or sorbitan.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethiotate, imidazolinium derivatives, methyltaurate, sarcosinate, fatty acid amide. Ethersulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanol amides, vegetable oil lanolin derivatives or ethoxylated glycerol, fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples. The following Examples and Experimental Examples are only for more specifically describing the present invention, and the scope of the present invention is not limited by them according to the gist of the present invention. It will be obvious to him.

Preparation Example 1 Preparation of Soru-Jin Extract

50 g of sorbus was immersed in 200 ml of 100% ethanol and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

Preparation Example 2 Preparation of Strobe Pine Bark Extract

50 g of strobe pine bark was taken and immersed in 200 ml of 100% ethanol and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

Experimental Example 1 Inhibitory Effect of Tyrosinase Activity

The following experiments were conducted to investigate the effects of the extracts of Soru-Jin and Strobe-Branberry on the inhibitory activity of tyrosine enzyme tyrosinase.

Tyrosinase was isolated and purified from mushrooms and was purchased from SIGMA. Tyrosine, a substrate, was dissolved in 0.05M sodium phosphate buffer (pH 6.8) and used as a 0.1 mg / ml solution.

Sorujeuk extract and strobe pine bark extract prepared in Preparation Examples 1 and 2 were used as a sample solution after melting to adjust the concentration in a buffer solution. In addition, 0.5ml of the sample tyrosine solution was added to the test tube in order to determine the effect of the extract and reacted at 37 ° C for 10 minutes. At this time, the control is 0.5ml of buffer solution. The test tube containing the reaction solution was placed on ice and quenched to stop the reaction, and the absorbance at 475 nm was measured with a spectrophotometer.

The inhibitory effect of tyrosinase activity at each concentration of the extracts from Soru-Ji extract and Strobe Pine Bark was calculated by the following Equation 1, and the results are shown in Table 1 below.

Tyrosinase activity inhibition rate (%) = 100- (absorbance at each concentration / absorbance of control × 100)

Concentration (㎍ / ml) Tyrosinase activity inhibitory effect (%) Strobe Pine Sorousum Extract 10 8.5 6.1 20 11.9 7.2 50 27.4 10.4 100 31.0 15.6 200 52.7 24.8

In the results of Table 1, the strobe pine bark extract showed an excellent inhibitory effect on tyrosinase activity, whereas the Soru-Zip extract showed a slight inhibition of tyrosinase activity compared to the strobe pine bark extract.

Experimental Example 2: Measurement of SCF expressed on keratinocyte membrane by ultraviolet stimulation

After seeding 1.5 million keratinocytes on a 10 cm plate and incubating for 72 hours, the medium is exchanged with PBS (Phosphate Buffered Saline), UVB is irradiated at 20 mJ / cm ² , and PBS is immediately exchanged with the medium. The extracts were added therein for 24 hours, and the cells were recovered after incubation for 24 hours, and the cells were diffused into 200 µl of 50 mM phosphate buffer (pH7.8) + protease inhibitor solution, followed by ultrasonic crushers for 5 seconds at 30 ° C. 5 times for 30 seconds. Shredded. This was centrifuged at 10,000 g, 20 minutes at 4 ° C, and the supernatant was further centrifuged at 100,000 g, 60 minutes at 4 ° C. The obtained pallet was dissolved in 100 µl of 25 mM phosphate buffer (pH6.8) + 0.1% Triton-X100 solution to obtain a membrane fraction protein extract. The protein amount of this solution was measured by the usual method, and the SCF amount was also measured to obtain a value of the SCF amount / protein mass.

The results are as shown in the following Table 2, in the untreated group irradiated only with ultraviolet light, the value of the stem cell factor was measured at 7.540. When the SCF content of the extract from Sorbus extract and Strobe pine bark extract was compared, Compared to the pine bark extract, it showed a very good SCF inhibitory effect.

SCF (pg) / protein (ug) Untreated 7.540 Soruzin Extract 0.01% 2.610 Soruzin Extract 0.05% 0.840 Strobe Pine Extract 0.01% 5.800 Strobe Pine Bark Extract 0.05% 4.600

 However, the extract of Strobe Pine Bark could inhibit the expression of SCF, but the effect was insignificant, and the Soru-Zip extract induced the UV rays to significantly inhibit the expression of SCF, which transmits the signal in melanocytes. Could.

Experimental Example 3: Antioxidant Action Test

In order to test the anti-oxidative effect of the extracts from Soru-Ji and Strobe-Branberry Bark, it was measured using DPPH solution, which is a method of measuring antioxidant activity.

100 µl of Soru-Ji extract and Strobe Pine extract 200mg / ml and DPPH solution, an antioxidant measurement solution, were mixed for 30 minutes at 37 ° C, and then absorbance was measured at 517 nm using an ELISA reader.

Antioxidant activity (%) was based on Equation 2, the results are shown in the following [Table 3].

% Antioxidant activity = (1-treated absorbance / untreated absorbance) × 100

Sample Name Antioxidant activity (%) Sorousum Extract 29.1% Strobe Pine Tree Extract 77.5%

As can be seen from the above results, the Soru-Ji extract used in the present invention has an antioxidant activity of 29.1%, the effect of which is low, strobe pine bark extract is 77.5%, it can be seen that the antioxidant activity is excellent.

Preparation Example 3 Examples and Comparative Examples

To the cosmetic composition having a composition ratio of the following [Table 4] was prepared an Example and Comparative Example containing the above-mentioned Soru-Ji extract and Strobe Pine extract with the following composition.

ingredient Example (% by weight) Comparative Example 1 (% by weight Comparative Example 2 (wt%) Comparative Example 3 (wt%) Sorousum Extract 2.0 4.0 - Strobe Pine Tree Extract 2.0 - 4.0 glycerin 5.0 5.0 5.0 5.0 Liquid paraffin 10.0 10.0 10.0 10.0 Polysorbate 60 2.0 2.0 2.0 2.0 Sorbitan sesquirololeate 1.0 1.0 1.0 1.0 Squalane 5.0 5.0 5.0 5.0 Beeswax 10.0 10.0 10.0 10.0 Triethanolamine 0.5 0.5 0.5 0.5 Propylene glycol 1.0 1.0 1.0 1.0 Carboxyl Vinyl Polymer 1.5 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance system 100 100 100 100

Experimental Example 4: Whitening Effect

In order to measure the whitening effect of the present invention, 40 healthy women in their 30s and 40s for 1 month were divided into four groups of 10 people each of Example 1 and Comparative Examples 1, 2, and 3 for 1 month. The black and white degree of skin was apply | coated and the effect was judged using the color difference meter (Minolta CR2002). L *, a *, b * color system is used to display color. In this experiment, L * value (brightness) was mainly used as an index. The L * value represents black (L * = 0) to white (L * = 100), and ΔL * after one month was obtained, and is shown in the following [Table 5].

Test substance ΔL * Example 10.1 Comparative Example 1 2.81 Comparative Example 2 4.62 Comparative Example 3 0.34

As can be seen from the above [Table 5], it can be seen that the embodiment containing the composition of the present invention shows the best whitening effect.

That is, when the strobe pine bark extract alone is contained in the cosmetic as in Comparative Example 2, it is judged that the effect of reducing the expression level of the stem cell factor (SCF) is low, which is less effective in skin whitening,

When it is contained in the cosmetics alone as an extract from Comparative Example 1, it is judged that the tyrosinase activity inhibition rate is lowered and the whitening effect is very inferior.

However, by supplementing the strobe pine bark, which has a low effect of inhibiting the expression of stem cell factors, as in the embodiment, by using a mixture of Soru-Zi extract with high suppression rate of stem cell factor expression, it has an excellent synergistic effect on skin whitening. Judging.

In addition, the extracts used in the present invention is expected to produce an excellent synergistic effect on skin whitening when used in combination with extracts with low inhibition rate of stem cell factor expression in conventional skin whitening cosmetic composition.

Experimental Example 5: Skin Safety Test

In order to confirm the skin safety of the present invention in 20 women in their 20s and 30s who have never had a hypersensitivity reaction to skin irritation in the past and do not have skin disease or skin allergy symptoms, The skin patch test was done. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was dropped in a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then sealed in the forearm inner part of the test subject. The patch was marked for 24 hours, the patch was removed, and the test site was marked with a pen. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. Skin response was determined according to the regulations of the International Contact Dermaitis Research Group (ICDRG), and the mean skin response rate was calculated according to <Equation 3>. The results are shown in Table 7 below.

[Evaluation Criteria and Score of Skin Response] Symbol score Evaluation standard - 0 No response ± 0.5 Faint or light erythema + One Boundary but weak erythema, edema and papules ++ 2 Pronounced erythema, papules and vesicles +++ 3 Severe erythema and alveolar, scleroderma

Average skin reactivity (%) = (score * reactions * 100 * 1/2) / [3 (maximum score) * total number of subjects (n)]

Test substance After 1 hour After 24 hours Reactivity (%)
(n = 20) ± + ++ ± + ++ Example - - - - - - 0.00 Comparative Example 1 - - - - - - 0.00 Comparative Example 2 - - - - - - 0.00 Comparative Example 3 - - - - - - 0.00

As can be seen in Table 7, in the skin patch test, it can be seen that the extracts and extracts of strobe pine bark are very excellent substances without skin irritation.

Hereinafter, as an example of the cosmetic formulation of the present invention, a soft lotion, a nourishing lotion, a nourishing cream, a massage cream, and the like containing the sorbus extract and the strobe pine extract of the present invention are exemplified, but the formulation of the cosmetic of the present invention is not limited thereto. It doesn't happen.

Formulation Example 1: Softener (Skin Lotion)

As shown in the following table, the softening lotion was prepared according to a conventional method.

Compounding ingredient Content (% by weight) Sorousum Extract 2.0 Strobe Pine Tree Extract 2.0 1.3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2. Nutrients (Milk Lotion)

Nutrients were prepared according to a conventional method as shown in the table below.

Compounding ingredient Content (% by weight) Sorousum Extract 2.0 Strobe Pine Tree Extract 2.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Formulation Example 3. Nutrition Cream

Nutritious cream was prepared according to a conventional method as shown in the following table.

Compounding ingredient Content (% by weight) Sorousum Extract 2.0 Strobe Pine Tree Extract 2.0 Cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.1 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 4 Massage Cream

Massage cream was prepared according to a conventional method as shown in the table below.

Compounding ingredient Content (% by weight) Sorousum Extract 2.0 Strobe Pine Tree Extract 2.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5 Pack

Packs were prepared according to conventional methods as shown in the table below.

Compounding ingredient Content (% by weight) Sorousum Extract 2.0 Strobe Pine Tree Extract 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

Claims (4)

A whitening cosmetic composition containing strobe pine extract and sorbus extract as active ingredients. The method according to claim 1, wherein the strobe pine bark extract and rubbing extract, Cosmetic composition for skin whitening, characterized in that contained in 0.001 ~ 10.0% by weight based on the total weight of the composition A cosmetic composition for inhibiting the expression of stem cell factor containing Soru-Jin extract as an active ingredient. The composition according to any one of claims 1 to 3, wherein the composition is a softener, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder. Cosmetic composition for skin whitening, characterized in that it has one formulation selected from the group consisting of.