KR101005556B1 - A cosmetic composition comprising an extract of saxifraga caespitosa var. uniflora and a preparation method of the extract - Google Patents

Abstract

The present invention is a polar plant saxipraga casphytosha bar. Uni Flora (Saxifraga caespitosa var. Uniflora) relates to a cosmetic composition and a method of manufacturing the same comprising the extract, it may be useful in cosmetic compositions for the prevention of skin aging, skin improvement and protection.

Cosmetics, saxpraga, caspitosha bar. Uniflora, Antioxidant, Cell Protection, Whitening, Wrinkles

Description

Saxpraga Caspitosha Bar. Cosmetic composition comprising a uniflora extract and the saxipraga casphytosha bar. A manufacturing method of a uniflora extract A COSMETIC COMPOSITION COMPRISING AN EXTRACT OF SAXIFRAGA CAESPITOSA VAR. UNIFLORA AND A PREPARATION METHOD OF THE EXTRACT}

The present invention is a saxipraga casphytosha bar excellent in cell protection, antioxidant, wrinkle improvement and whitening effect. Cosmetic composition comprising a uniflora extract and the saxipraga casphytosha bar. It relates to a method of preparing a uniflora extract.

Skin protects the body from external stimuli. These external stimuli, that is, environmental, physical and chemical stimuli, deteriorate skin function and promote skin aging. Therefore, efforts to maintain healthy and beautiful skin while preventing such skin phenomena continue. Recently, research efforts to improve skin have been actively studied for developing materials using plant extracts that have no side effects, are friendly to human beings, and can be used safely regardless of constitution.

In general, functional cosmetics containing plant extracts are prepared by extracting plants growing under conditions suitable for plant growth with various solvents. The conditions under which plants can grow vary widely from tropical rainforest climate to cold climates. However, there are unique flora that can grow according to climatic conditions such as sunshine, temperature, moisture, and fertility of the soil. The active ingredient inside the plant depends on the climate and soil. In particular, plants that grow in extreme climatic conditions often have their own unique biomechanical mechanisms, so they often have different components from plants of the same species living in different climates.

Polar plants are plants that grow in the north and south of the polar region, and are mainly abundant in phytopods and lichens. In addition, as a seed plant, dwarf shrubs and short perennials are predominant, with only a few known species. Perennial herb has a high degree of drainage of chromosomes, and adolescents often reproduce asexually. There are many plant species in common with alpine vegetation, but many of the indigenous subspecies grow only in the polar regions. In terms of the life style of plants, annual high-growth plants decrease, and the proportion of indicator plants such as moss increases. The dwarf tree growing in the northern hemisphere has many plants belonging to the rhododendron.

Saxpraga Caspitosha Bar. Uniflora is a perennial plant native to the Arcticaceae family of plants belonging to the genus Pantheus genus, 3-15 cm long, with several straight flowers. The leaves are 5-10 mm hard and very hairy. The tip of the leaf is divided into three lobes. At the top of the stem, two to ten flowers bloom, each of which consists of five white petals, which resemble a bell and when in full bloom, resemble a star. Saxipraga's genus is very diverse. Among them, Saxif Laga Caspitosha Bar. Uniflora has a well-established underground root system for storing carbohydrates, allowing it to adapt quickly to cold climates such as tundra. Saskia, a sapphire, is a caspitosha bar. Uniflora grows among the rocky slopes and crevices of the tundra. Saxpraga Caspitosha Bar. The results of scientific experiments reported on the efficacy of UniFlor are inadequate. In addition, there have been no reports on the application of cosmetic ingredients.

Thus, the inventors of the saxi saga caspitosha bar. Focusing on the evaluation of the efficacy of the uniflora extract, the present invention was completed by continuing the study on the applicability as a pure natural cosmetic.

The present invention is saxipraga casphytosha bar having a cytoprotective, antioxidant, anti-wrinkle and whitening effect. It is an object of the present invention to provide a cosmetic composition comprising the extract ( Saxifraga caespitosa var. Uniflora ) and a method for producing the same.

The present invention, saxpraga caspitosha bar. Cosmetic composition comprising a extract of Saxifraga caespitosa var. Uniflora and the saxifraga caspitosha bar. It provides a method of preparing a uniflora extract.

The cosmetic composition of the present invention is based on the total weight of the composition, the saxipraga caspitosha bar. The extract of Uniflora may be included in an amount of 0.1 to 10.0% by weight.

In addition, the present invention is a saxipraga caspitosha bar. Immersing the uniflora extract in at least one extraction solvent selected from the group consisting of purified water, C1 to C4 alcohol, and C1 to C5 alkylene glycol for 3 to 30 days to form an immersion solution; And filtering the immersion solution to the saxy praga casphytosha bar. Saxipraga casphytosha bar comprising the step of obtaining a uniflora extract. It provides a method of preparing a uniflora extract.

Since the cosmetic composition of the present invention uses a natural extract, it is free from skin irritation, and has a cytoprotective effect, antioxidant, anti-wrinkle and whitening effect, and thus is very effective in preventing skin aging, skin improvement and protection.

Hereinafter, the present invention saxpraga caspitosha bar. It describes a cosmetic composition comprising a uniflora extract.

The cosmetic composition of the present invention is natural saxy praga caspitosha bar. By containing uniflora extract, it protects the skin from various external stimuli to make the skin soft and shiny and helps to improve skin function.

Cosmetic composition of the present invention, saxipaga caspitosha bar relative to the total weight of the composition. It is preferable to include the uniflora extract in 0.1 to 10.0% by weight. If the above range is satisfied, a significant effect is obtained. In addition, when considering the intrinsic smell, color, etc. of the extract in the final product is advantageously included in the above range.

The saxpraga caspitosha bar. Uniflora extract is preferably extracted using at least one solvent selected from the group consisting of purified water, C1 to C4 alcohol and C1 to C5 alkylene glycol.

As a product to which the cosmetic composition of the present invention can be added, for example, various skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, nutrition lotions, massage creams, nutrition creams, hand creams, foundations, makeups Base, twin cake, mascara, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser and the like, but is not limited thereto. When the cosmetic composition of the present invention is prepared in a formulation of the form described above, it may contain various bases and additives necessary and appropriate for the formulation of the formulation. In addition, the type and amount of these components can be easily selected by those skilled in the art.

Hereinafter, the saxy praga caspitosha bar of the present invention. The manufacturing method of the uniflora extract is demonstrated.

In addition, the present invention is a) saxy praga casphytosha bar. Immersing uniflora ( Saxifraga caespitosa var.uniflora ) in at least one extraction solvent selected from the group consisting of purified water, C1 to C4 alcohol and C1 to C5 alkylene glycol for 3 to 30 days to form an immersion solution ; And b) the sap solution is filtered and the saxy praga caspitosa bar. Obtaining a uniflora extract.

In step a), the saxima is a caspitosha bar. Preferably, the uniflora is in a dried state. The deposition time is preferably 10 to 20 days.

The extraction solvent, the dried saxipraga caspitosha bar. It is preferable to use 10-20 times (w / w) with respect to the weight of uniflora.

On the other hand, the C1 to C4 alcohol is preferably ethanol, the C1 to C4 alkylene glycol is preferably at least one member selected from the group consisting of 1,3-butylene glycol, propylene glycol and pentylene glycol. .

In addition, when the extraction solvent is a mixed solvent of the alcohol and alkylene glycol, it is preferable that their mixing ratio (w / w) is 6: 4 to 9: 1. In the case of the mixed solvent of the alcohol and the alkylene glycol, when the content is in the above-described range, it has a more efficient polarity to extract only the desired useful component. Here, the concentration of the alcohol used in the mixed solvent of the alcohol and alkylene glycol is preferably from 60 to 100%.

In addition, the extraction solvent may be a mixed solvent of the purified water and the alkylene glycol, the mixing ratio thereof is preferably 3: 7 to 1: 9.

When the extraction solvent is a single solvent, preferably ethanol, in the case of a mixed solvent, a mixed solvent of alcohol and alkylene glycol, preferably ethanol and 1,3-butylene glycol, ethanol and propylene glycol or ethanol and pen It is a mixed solvent of styrene glycol, More preferably, it is a mixed solvent of ethanol and 1, 3- butylene glycol or ethanol and pentylene glycol.

The step b) preferably further comprises the step of removing the extraction solvent.

Saxipraga Caspitosha bar of the present invention. The manufacturing method of the uniflora, said saxipraga caspitosha bar. Treating uniflora extract to human fibroblasts to investigate the cytotoxic effect on skin irritation, the saxipraga casphytosha bar. Treating the uniflora extract to human fibroblasts to investigate the cytoprotective effect against external stimuli, the saxipraga casphytosha bar. Treating uniflora extracts to human fibroblasts to investigate skin antifouling effect according to collagen biosynthesis, and to investigate the skin whitening improvement effect due to tyrosinase inhibition. It may include.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples, Test Examples, Examples, and the like. However, the production examples, test examples, examples, and the like described below are for illustrating the present invention, and the present invention is not limited thereto and may be variously modified and changed.

Preparation Examples 1 to 4, Comparative Preparation Examples 1 to 4: Saxypraga Caspitosha Bar. Preparation of Uniflora Extract

Saxpraga Caspitosha Bar. The uniflora and rock odors were washed with water, dried with gauze, dried and chopped to prepare. Saxipraga Caspitosha bar prepared above. Uniflora and rock odors were aged by dipping for 2 weeks in the mixed solvent present in the contents shown in Table 1 and the contents shown in Table 1. After that, the filtrate was filtered through a filter paper (5C, 185 mm commercially available from Toyoroshi Kaisha, Ltd., Japan), and ethanol was evaporated to obtain filtrate saxpraga caspitosha bar. Uniflora extract was obtained.

Dried saxpraga caspitosha bar. Uniflora (g) Dried Rock Odor (g) Extraction solvent (g) Type (mixing ratio) content Preparation Example 1 20 - b-1: b-2 = 7: 3 980 Preparation Example 2 20 - b-1: b-2 = 6: 4 980 Preparation Example 3 20 - b-1: b-3 = 7: 3 980 Production Example 4 20 - b-1: b-3 = 6: 4 980 Comparative Production Example 1 - 20 b-1: b-2 = 7: 3 980 Comparative Production Example 2 - 20 b-1: b-2 = 6: 4 980 Comparative Production Example 3 - 20 b-1: b-3 = 7: 3 980 Comparative Production Example 4 - 20 b-1: b-3 = 6: 4 980

b-1: 70% ethanol b-2: 1,3-butylene glycol

b-3: propylene glycol

Test Example 1: Saxpraga Caspitosha Bar. Cytotoxicity Measurement of Uniflora Extract

Preparation Example 1 Safety was evaluated through a cytotoxicity test for Comparative Preparation Example 1.

Cell culture

CCD-1064sk human fibroblasts purchased from ATCC were used with Iscove's modified Dulbeco's medium with 10% fetal calf serum (FBS) and 1% penicillin-streptomycin. ) Were cultured in a 10 cm cell culture dish with 10 ml of medium at 37 ° C. in a 5% CO ₂ incubator so that the cells were 70-80% confluent. The medium was changed twice a week, and the cells reached the confluence were maintained by passage culture after trypsinization using trypsin-EDTA. Human fibroblasts cultured above were divided into 96 × multiwell plates at 1 × 10 ⁴ cells / well and incubated for 24 hours. The medium of cultured cells was replaced with 2% FBS medium and Saxipraga Casphytosha bar. Preparation Example 1, a uniflora extract, and Comparative Preparation Example 1, a rock odor extract, were each diluted in a medium for each treatment concentration, and then incubated in a CO ₂ incubator for 48 hours.

Test Example 1-1: Measurement of Cytotoxic Effect (MTT assay)

To the cultured cells, MTT stock solution (5 mg / ml) dissolved in PBS (phosphate buffered saline) was diluted 10-fold in fresh medium, and 100 µl of each solution was added to each well of the cell at 37 ° C. The reaction was carried out for 4 hours. After the reaction, the living cells were dehydrated by the dehydrogenase present in the mitochondria and the yellow water-soluble substrate MTT [3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium-bromide] was insoluble in water. Since it is reduced to (MTT-formazan) crystals, the amount of this can be measured to measure cell viability. Remove the culture supernatant and add 200 μl of DMSO to each well to dissolve the MTT-formenic crystals produced in the cells, and then use the absorption spectrophotometer (UVmax, Molecular Device, USA) to filter the maximum absorbance of the formazin. The absorbance of the difference value was obtained by measuring the OD value at a wavelength of 540 nm as a test filter and a 630 nm wavelength as a reference filter (The EMBO Journal (2002) 21, 2407). -2417 and many more). Cytotoxicity is expressed as a percentage of absorbance.

※ Cell survival rate (%) = (Sample absorbance / untreated group absorbance) × 100

The standard deviation was obtained by calculating the average of the absorbance of the untreated group and the absorbance of the sample treated group based on raw data.

MTT assay OD Mean ± SD
(% of control) Sample concentration (W / W%) Control One 0.5 0.1 0.05 0.01 Preparation Example 1 0.479 ± 0.08
(100.0) 0.454 ± 0.012
(94.61) 0.481 ± 0.013
(100.41) 0.499 ± 0.015
(104.00) 0.510 ± 0.015
(106.40) 0.507 ± 0.011
(105.78) Comparative Production Example 1 0.479 ± 0.08
(100.0) 0.461 ± 0.015
(96.08) 0.471 ± 0.012
(98.27) 0.484 ± 0.018
(100.98) 0.490 ± 0.011
(102.23) 0.492 ± 0.007
(102.54)

Test Example 1-2: Measurement of Cytotoxic Effects (Neutral Red assay)

Neutral Red (Neutral Red, Sigma-Aldrich, USA) was added to the cultured cells and the cells were reacted for 3 hours. Neutral Red is concentrated in the lysosomes of completely living or intact cells. After the reaction, cells were fixed with 1.0% formalin / 1.0% potassium chloride and then neutral red was extracted by adding 1.0% acetic acid / 50% ethanol solution. The extracted neutral red was measured by absorbance spectrophotometer (UVmax, Molecular Device, USA) to measure the cytotoxicity value of each concentration of 540-630 nm. Table 2 shows the cytotoxicity expressed as a percentage of absorbance and 3 lots. (lot) was performed three times.

※ Cell survival rate (%) = (Sample absorbance / untreated group absorbance) × 100

NR assay OD Mean ± SD
(% of control) Sample concentration (W / W%) Control One 0.5 0.1 0.05 0.01 Preparation Example 1 0.303 ± 0.010
(100.0) 0.286 ± 0.013
(94.52) 0.307 ± 0.018
(101.30) 0.318 ± 0.009
(104.93) 0.324 ± 0.014
(107.08) 0.326 ± 0.020
(107.74) Comparative Production Example 1 0.303 ± 0.010
(100.0) 0.298 ± 0.020
(98.49) 0.304 ± 0.014
(100.31) 0.308 ± 0.013
(101.79) 0.311 ± 0.007
(102.79) 0.315 ± 0.013
(104.11)

The standard deviation was obtained by calculating the average of the absorbance of the untreated group and the absorbance of the sample treated group based on raw data.

As shown in Table 2 and Table 3, MTT ₅₀ , NR ₅₀ value of Preparation Example 1 of the present invention is 1% or more, it can be seen that the raw material is safe compared to Comparative Preparation Example 1.

Test Example 2: Saxpraga Caspitosha Bar. Measurement of Cytoprotective Effect of Uniflora Extract

Cytoprotective effect experiments were performed on SDS (Sodium dodecyl sulfate) cytotoxicity against Preparation Example 1 and Comparative Preparation Example 1.

Human fibroblasts were cultured in 37 ° C., 5% CO ₂ incubator with IMDM with 10% fetal calf serum (FBS) and 1% penicillin-streptomycin. Cultured human fibroblasts were dispensed at 1 × 10 ⁴ cells / well in 96-multiwell plates and incubated for 24 hours. The medium of cultured cells was replaced with 2% FBS medium, and 0.002% of SDS (Sodium dodecyl sulfate) was pretreated for 1 hour, followed by dilution of Preparation Example 1 and Comparative Preparation Example 1 in the medium for each treatment concentration for 24 hr. Incubated in a CO ₂ incubator. In cultured cells, MTT stock solution (5 mg / ml) dissolved in PBS (phosphate buffered saline) was diluted 10-fold in fresh medium, and 100 µl of each solution was added to each well of the cell, followed by 4 hours at 37 ° C. Reacted. After the reaction, the living cells were dehydrated by the dehydrogenase present in the mitochondria, and the yellow water-soluble substrate MTT [3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium-bromide] was insoluble in water. Since it is reduced to crystals, the amount of this can be measured to determine cell viability. Remove the culture supernatant and add 200 μl of DMSO to each well to dissolve the MTT-formenic crystals produced in the cells, and then use the absorption spectrophotometer (UVmax, Molecular Device, USA) to filter the maximum absorbance of the formazin. The OD value at a wavelength of 540 nm and the OD value at a wavelength of 630 nm as a reference filter were measured to determine the absorbance of the difference (Ref. EMBO Journal (2002) 21, 2407-2417 et al.). The cytoprotective effect is expressed as a percentage of absorbance.

※ Cell survival rate (%) = (Sample absorbance / untreated group absorbance) × 100

The standard deviation was obtained by calculating the average of the absorbance of the untreated group and the absorbance of the sample treated group based on raw data.

MTT assay OD Mean ± SD
(% of control) Sample concentration (W / W%) Control SDS Only SDS + 0.01 SDS + 0.05 SDS + 0.1 SDS + 0.5 Preparation Example 1 0.426 ± 0.009
(100.0) 0.222 ± 0.015
(52.18) 0.230 ± 0.019
(53.98) 0.261 ± 0.011
(61.33) 0.308 ± 0.021
(72.29) 0.322 ± 0.012
(75.49) Comparative Production Example 1 0.532 ± 0.009
(100.0) 0.222 ± 0.013
(52.18) 0.226 ± 0.014
(53.12) 0.240 ± 0.015
(56.76) 0.272 ± 0.014
(63.76) 0.284 ± 0.012
(66.58)

As shown in Table 4, it can be seen that the cell protective effect of Preparation Example 1 of the present invention promotes the cell protective effect against external stimulation than Comparative Preparation Example 1.

Test Example 3 Saxy Praga Caspitosha Bar. Antioxidant Effect of Uniflora Extracts

Antioxidant effect test was carried out for Preparation Example 1 and Comparative Preparation Example 1.

<Measurement of anti-hydroxyl radical activity (%) by DPPH (1,1-diphenyl-2 picrylhydrazyl) radicals>

Reactions by free radicals are almost always radical reactions, including the process of automatic oxidation. Antioxidants are responsible for blocking the automatic oxidation in vivo. The greater the reducing power as an electron-giving ability, the stronger the antioxidant. A reagent that measures the reducing power of antioxidants is the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. DPPH exists as a radical of a stabilized structure of the nitrogen center in the compound. The maximum absorption at 517 nm is shown and the reduction is absent at 517 nm. DPPH (D9132, SIGMA) was dissolved in a 6: 4 (v / v) mixed solvent of methanol and distilled water, and the DPPH solution was adjusted to have an absorbance of about 0.6 at 513 nm. 2 ml of DPPH solution was added to 1 ml of the diluted sample and mixed. After standing at room temperature for about 1 hour, absorbance at 513 nm was measured using an absorption spectrophotometer (UVmax, Molecular Device, USA) and the results are shown in Table 5 below.

DPPH assay OD Mean ± SD
(% of control) Sample concentration (W / W%) Control One 0.5 0.25 0.1 0.05 Preparation Example 1 0.862 ± 0.008
(0.00) 0.161 ± 0.007
(81.32) 0.295 ± 0.015
(65.78) 0.520 ± 0.008
(39.68) 0.621 ± 0.012
(27.96) 0.762 ± 0.007
(11.60) Comparative Production Example 1 0.862 ± 0.008
(0.00) 0.211 ± 0.009
(75.52) 0.490 ± 0.011
(43.16) 0.712 ± 0.014
(17.40) 0.761 ± 0.011
(11.72) 0.812 ± 0.011
(5.80)

As shown in Table 5, Preparation Example 1 showed an excellent antioxidant effect.

Test Example 4: Saxpraga Caspitosha Bar. Measurement of Tyrosinase Activity and Inhibition of Melanin Synthesis by Uniflora

Test Example 4-1. Tyrosinase Activity Inhibition Test

Preparation Example 1 and Comparative Preparation Example 1 Tyrosinase activity inhibition test was conducted to investigate the whitening activity. For the experiment, tyrosinase enzyme solution was prepared by dissolving mushroom tyrosinase (mushroom tyrosinase, T-3824, 1530U / mg, Sigma) in phosphate buffer (pH 6.5) to 1000 U / ml, and the substrate solution L-tyrosine (L-tyrosine, 45160-0410, Junsei chemical co. Ltd) was prepared by dissolving in phosphate buffer (pH 6.5) to 1.5 mM.

Preparation Example 1 and Comparative Preparation Example 1 were prepared by diluting each sample in a buffer at different concentrations. 170 μl of sample solution and 10 μl of tyrosinase enzyme solution were added thereto, and the mixture was left at 37 ° C. for 10 minutes. 20 μl of substrate solution was added thereto, followed by reaction for 10 minutes at 37 ° C., and then left for 5 minutes in ice, and then absorbance was measured at wavelength 490 nm using an absorption spectrophotometer (UVmax, Molecular Device, USA). Substituting the absorbance measured in the above Equation 1 to calculate the tyrosinase activity inhibition rate is shown in Table 6 below. A solution prepared by adding phosphate buffer (pH 6.5) instead of the sample solution was used as a blank test solution, and a solution prepared by adding phosphate buffer (pH 6.5) instead of the substrate solution was used as a calibration solution.

&Quot; (1) &quot;

Tyrosinase activity inhibition rate (%) = 100-(A-A ') / (B-B') X 100

A: absorbance after the liquid reaction

B: absorbance after reaction of blank test solution

A ': correction liquid of the test liquid

B ': Correction amount of blank

Tyrosinase
Activity inhibition measurement OD Mean ± SD
(% of control) Sample concentration (W / W%) Control 5 2.5 One 0.5 Preparation Example 1 0.225 ± 0.008
(0.00) 0.047 ± 0.08
(79.09) 0.078 ± 0.011
(65.29) 0.126 ± 0.004
(43.94) 0.188 ± 0.008
(16.35) Comparative Production Example 1 0.225 ± 0.008
(0.00) 0.088 ± 0.010
(60.85) 0.142 ± 0.013
(36.82) 0.192 ± 0.007
(14.57) 0.205 ± 0.011
(8.79)

Test Example 4-2: Saxy Praga Caspitosha Bar. Melanin synthesis inhibition test according to the concentration of uniflora extract

For Preparation Example 1 and Comparative Preparation Example 1, the whitening effect was measured using B-16 melanoma cells (prepared Yonsei University Wonju Medical College). As a measuring method, B-16 melanoma cells derived from black mice were added to a 6 well tissue culture dish with 2 ml of 2 x 10 ⁴ cells / well in DMEM containing 10% FBS (GIBCO., USA). 5% CO ₂ , and incubated for 24 hours under 37 ℃ conditions. After incubation, the medium was removed and replaced with DMEM containing 10% FBS, 2uM α-MSH (Sigma., USA, Melanocyte stimulating Hormone) and 2 mM theophylline (Sigma., USA, theophylline), and then cultured wild ginseng root muscle extract Were diluted in the same medium and added, respectively, and then cultured under 5% CO ₂ , 37 ° C. until the cells were at least about 80% at the bottom of the well. After incubation, the medium was removed, washed with PBS (Sigma., USA, Phosphate buffered Saline) and treated with trypsin to recover the cells. The recovered cells were centrifuged at 5000 rpm for 10 minutes and the supernatant was removed. Cells from which the supernatant was removed were dried for 24 hours at 60 ° C. incubator and lysed melanin by adding 1N NaOH. The dissolved melanin was measured for absorbance at wavelength 490 nm using an absorption spectrophotometer (Uvmax, Molecular Device, USA).

<Equation 2>

Tyrosinase activity inhibition rate (%) = 100-(A-A ') / (B-B') X 100

A: absorbance after the liquid reaction

B: absorbance after reaction of blank test solution

A ': Correction liquid of the test liquid

B ': Correction amount of blank

Melanin synthesis
Suppression measurement OD Mean ± SD
(% of control) Sample concentration (W / W%) Control 0.25 0.1 0.05 0.01 Preparation Example 1 0.267 ± 0.011
(0.00) 0.073 ± 0.015
(72.61) 0.113 ± 0.09
(57.60) 0.184 ± 0.013
(30.96) 0.239 ± 0.016
(10.32) Comparative Production Example 1 0.267 ± 0.011
(0.00) 0.153 ± 0.009
(42.59) 0.181 ± 0.018
(32.08) 0.255 ± 0.011
(4.32) 0.259 ± 0.009
(2.81)

As shown in Table 6 and Table 7, Preparation Example 1 inhibited tyrosinase activity in a concentration-dependent manner, and was excellent in inhibiting melanin production in the cell.

Test Example 5: Saxpraga Caspitosha Bar. Collagen Biosynthesis Determination of Uniflora Extracts

One of the causes of skin wrinkles is a deficiency of collagen, or collagen. Collagen is a major protein that makes up the skin dermis and plays a role in maintaining skin structure and elasticity. Collagen is known to show a decrease in production with increasing age and to increase the decomposition to induce depression of the dermal dermal layer to produce wrinkles of the skin.

<Measurement of collagen biosynthesis amount>

In order to investigate the anti-wrinkle effect of the cosmetics according to the present invention, a collagen biosynthesis promoting test (Sircol ^™ Soluble Collagen Assay Kit) using fibroblasts was performed.

The cultured human fibroblasts were cultured in a medium containing 5% FBS for 24 hours to be 1 × 10 ⁵ cells / well in a 12 well cell culture dish, and cultured in a medium containing 2% FBS. Preparation Example 1 was diluted for each treatment concentration, treated with cells, and cultured for 48 hours, and used in the following experiment. In order to confirm that the synthesis of collagen was actually increased in the fibroblasts, Biocolor's Sircol ^TM soluble collagen assay kit (Soluble Collagen Assay kit) was purchased, and the amount of collagen was measured according to the experimental method of the product manual. Sircol ^TM soluble collagen assay kit is a quantitative method that can be identified by combining collagen and staining reagent secreted into the culture medium during cell culture. Specifically, 1 ml of a dyeing reagent (a solution containing Sirius red reagent in picric acid) was added to 100 µl of the culture supernatant and allowed to react for 30 minutes (concentration of the culture solution or the culture supernatant during the test depending on the amount of collagen production). Sirius red is an anionic pigment that specifically binds to collagen. After reacting for 30 minutes, the reaction solution was centrifuged at 12,000 × g for 10 minutes to precipitate the collagen-pigment conjugate. 1.0 ml of alkaline reagent was added to the precipitate and dissolved for 5 minutes at room temperature. Absorbance of the solution was measured at 540 nm using an absorbance spectrophotometer (UVmax, Molecular Device, USA), and is shown in Table 8 below. The effect of promoting collagen biosynthesis was evaluated by comparing the absorbance of the test group.

sample OD Mean ± SD
(% of control) Sample concentration (W / W%) Control 0.01 0.05 0.1 0.5 Preparation Example 1 0.479 ± 0.048
(100) 0.490 ± 0.024
(102.24) 0.512 ± 0.031
(106.73) 0.529 ± 0.021
(110.38) 0.514 ± 0.031
(107.15) Comparative Production Example 1 0.479 ± 0.048
(100) 0.478 ± 0.029
(99.79) 0.497 ± 0.022
(103.70) 0.502 ± 0.029
(104.75) 0.511 ± 0.035
(106.52)

As shown in Table 8, Preparation Example 1 was confirmed that the collagen biosynthesis effect is superior to Comparative Preparation Example 1.

Example 1 Saxypraga Caspitosha Bar Preparation of Essences Containing Uniflora Extracts.

An essence comprising Preparation Example 1 was prepared according to the composition of Table 9 below.

Ingredient Name Content (% by weight) A 1,3-butylene glycol 1.0 glycerin 1.0 Carbomer 0.2 Glyceryl Methacrylate 0.35 Parabens 0.20 Purified water Balance B Potassium hydroxide 0.06 C PEG-6-hydrogenated castor oil 1.0 Spices 0.5 D Preparation Example 1 5.0 system 100.00

A was sufficiently dispersed and wetted to form a uniform gel, and neutralized by adding B. C was added to (A + B), uniformly stirred and solubilized, and then D was added at room temperature, stirred to distribute uniformly, and put into a container to produce a product.

Examples 2-4: Saxypraga casphytosha bar. Preparation of Softening Soap (Skin) Containing Uniflora Extract

Examples of the flexible longevity (skin) in the cosmetic composition containing Preparation Example 1 are shown in Table 10 below.

ingredient Example 2 Example 3 Example 4 Content (% by weight) Content (% by weight) Content (% by weight) Article 1 10.00 5.00 2.00 Butylene glycol 2.00 2.00 2.00 glycerin 3.00 3.00 3.00 Oleyl alcohol 2.00 2.00 2.00 Polysorbate 20 1.00 1.00 1.00 ethanol 6.00 6.00 6.00 antiseptic 0.5 0.5 0.5 incense 0.5 0.5 0.5 Purified water Balance Balance Balance system 100.00 100.00 100.00

Examples 5-7: Saxpraga caspitosha bar. Preparation of Nutrients (Milk Lotion) Containing Uniflora Extract

Formulation example of nutrient emulsion (milk lotion) in the cosmetic composition containing Preparation Example 1 is shown in Table 11.

ingredient Example 5 Example 6 Example 7 Content (% by weight) Content (% by weight) Content (% by weight) Preparation Example 1 10.00 5.00 2.00 Butylene glycol 4.00 4.00 4.00 glycerin 3.00 3.00 3.00 Oleyl alcohol 1.00 1.00 1.00 Polysorbate20 1.00 1.00 1.00 antiseptic 0.5 0.5 0.5 incense 0.5 0.5 0.5 Purified water Balance Balance Balance system 100.00 100.00 100.00

Examples 8-10: Saxpraga caspitosha bar. Preparation of a Cream Containing Uniflora Extracts

Formulation example of the cream in the cosmetic composition containing Preparation Example 1 is shown in Table 12 below.

ingredient Example 8 Example 9 Example 10 Content (% by weight) Content (% by weight) Content (% by weight) Preparation Example 1 10.00 5.00 2.00 Butylene glycol 2.00 2.00 2.00 glycerin 4.00 4.00 4.00 Potassium hydroxide 0.10 0.10 0.10 Tocopheryl acetate 1.0 1.0 1.0 Liquid paraffin 5.0 5.0 5.0 Squalane 5.0 5.0 5.0 Macadamia Nut Oil 10.0 10.0 10.0 Polysorbate 60 1.0 1.0 1.0 Sorbitan sesquioleate 1.0 1.0 1.0 Carbomer 0.3 0.3 0.3 antiseptic 0.5 0.5 0.5 incense 0.5 0.5 0.5 Purified water Balance Balance Balance system 100.00 100.00 100.00

Claims (8)

end The plant, the saxipraga caspitosa bar. Cosmetic composition comprising an extract of Saxifraga caespitosa var. Uniflora . The bar of claim 1, wherein the saximapragas casptosha bar is based on the total weight of the composition. Cosmetic composition, characterized in that it comprises 0.1 to 10.0% by weight of uniflora extract. The bar of claim 1, wherein the saximapra is a casptosha bar. Uniflora extract is a cosmetic composition, characterized in that the extraction using at least one solvent selected from the group consisting of purified water, C1 to C4 alcohol and C1 to C5 alkylene glycol. The cosmetic composition according to claim 1, wherein the cosmetic composition is used for antioxidant, skin wrinkle improvement, skin whitening and skin protection. The method of claim 1, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, nutrition lotion, massage cream, nutrition cream, hand cream, foundation, makeup base, twin cake, mascara, essence, nutrition Cosmetic composition, characterized in that formulated in the form selected from essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser. a) Sexy praga caspitosha bar. Immersing uniflora ( Saxifraga caespitosa var.uniflora ) in at least one extraction solvent selected from the group consisting of purified water, C1 to C4 alcohol and C1 to C5 alkylene glycol for 3 to 30 days to form an immersion solution ; And b) by filtering the immersion solution saxy praga caspitosha bar. Saxipraga casphytosha bar comprising the step of obtaining a uniflora extract. Method for preparing uniflora extract. The method of claim 6, wherein the extraction solvent is ethanol; Ethanol and 1,3-butylene glycol; Ethanol and propylene glycol; And ethanol and pentylene glycol; saxipraga casphytosha bar, characterized in that at least one selected from the group consisting of. Method for preparing uniflora extract. The method according to claim 6, wherein step b) saxipraga casphytosha bar further comprises the step of removing the extraction solvent. Method for preparing uniflora extract.