ES2420517B1 - Formulations with fibroblast stimulation activity containing extracts of Armeria pubigera and / or Saxifraga spathularis - Google Patents

Abstract

Formulations with fibroblast stimulation activity that contain extracts of pubertal armory and / or Saxophage spathularis. Cosmetic and / or dermatological formulations containing at least one plant extract of pubertal Armory and / or Sax�fraga spathularis. Use of these formulations and extracts of pubertal Armory and / or Saxophage spathularis to regenerate the dermal structure due to its capacity to stimulate human dermal fibroblasts.

Description

FORMULATIONS WITH ACTIVITY OF FIBROBLESTIC STIMULATION CONTAINING EXTRACTS OF PUBLIC ARMERIA YIO SAXIFRAGA SPATHULARIS.

FIELD OF THE INVENTION

The present invention relates to new cosmetic and / or dermatological formulations containing at least one plant extract of Armeria pubigera and / or Sax'fraga spafhularis capable of regenerating the dermal structure by its ability to stimulate human dermal fibroblasts.

BACKGROUND OF THE INVENTION

The dermis is the intermediate, resistant and elastic layer of the skin. This layer is constituted by connective tissue that has as fibroblast cell elements, mast cells and phagocyte cells (macrophages, histiocytes) and an extracellular matrix whose basic structure is a dense network of fibers, mainly of collagen and elastin, embedded in a colloidal gel or fundamental substance, composed of glycosoaminoglycans, hyaluronic acid, chondroitinsulfate and dermatansulfate, which embed a large amount of water. Also, in the dermis are nelViosae terminations, blood vessels, sweat glands and sebaceous glands.

The function of fibroblasts is the synthesis and maintenance of the extracellular matrix, producing: collagen, fundamental substance and fibrous proteins, such as fibronectin and laminin, and elastic fibers, formed predominantly by elastin and other proteins such as fibrillin.

The skin aging caused by chronological age, hormonal alterations or oxidative stress, produces, among other effects, a decrease in cell renewal and an alteration in the functioning of the fibroblasts of the dermis, which decrease the synthesis of the components of the extracellular matrix, which breaks the balance between the construction, reconstruction and repair of the dermis. As a result of these alterations there is a decrease in skin thickness, loss of elasticity, sagging and wrinkles.

It is important, therefore, the development of new products that combat the effects of aging, regenerating the dermal structure through fibroblast stimulation and, therefore, collagen synthesis.

Anneria pubigera (Plumbaginaceae) is an endemic plant on the northwest coast of the Iberian Peninsula (Spain and Portugal). Its cosmetic, pharmaceutical and / or food use as antioxidants / anti-radical / UV filters (ultraviolet) has been reported [ES200901652].

Sax�fraga spathularis (Plumbaginaceae) is an endemic plant in the northwest of the Iberian Peninsula (Spain and Portugal) and Ireland. Its internal use as an anti-inflammatory and analgesic has been reported for veterinary treatments (E. Blanco el al., El Caurel, plants and their inhabitants. Ethnobotanical study of the Sierra del Caurel (Lugo): the importance of plants for our ancestors; Caixa Galicia Foundation, A Coruña, 1996, 203) And its typical use for the treatment of forages (MP González-Hem�ndez et al., A proceedings of! he XXVI

Intemational Horticultural Congress, Toronto, 2002, 63-75). Also, reported its cosmetic, pharmaceutical and / or food use antioxidants / anti-radical / UV filters (ultraviolet) [ES200901653].

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DESCRIPTION OF THE INVENTION

The present invention relates to new cosmetic / dermatological formulations containing at least one plant extract of Armeria pubigera and / or Saxifraga spafhularis, capable of regenerating the dermal structure by its stimulation capacity on fibroblasts d Human chemicals.

The present invention describes the determination of stimulation capacity on human dermal fibroblasts from the extracts of Armerla pubigera and Sax�fraga spathularis.

The cosmetic formulations object of the present invention contain at least one extract of Armeria pubigera and / or Saxifraga spathularis.

Advantageously, this Armerla pubigera extract is an extract obtained according to the extraction procedure set out in the patent [ES200901652].

Advantageously, this extract of Saxifraga spathularis is an extract obtained according to the extraction procedure set out in the patent [ES200901653 [.

The cosmetic formulations object of the present invention contain:

-

of the order of 01-10% by weight of Armerla pubigera extract, and / or

-

of the order of 01-10% by weight of extract of Saxifraga spathularis.

The cosmetic formulations object of the present invention may include one

or several components of conventional known use in cosmetic and dermatological formulations, for example and without being limiting, colorants, preservatives, perfumes, oils, waxes, vitamins, UV filters, surfactants, emulsifiers, glycols, solvents, regulators of pH, etc. According to the knowledge of the state of the art, it will be known which formulation agents can be added to the formulations of the invention and in what quantities.

The formulations according to the invention can be presented in any form known in the field of cosmetogy and dermatology.

The present invention also relates to the use of formulations according to the invention to regenerate the dermal structure by fibroblast stimulation.

The present invention also relates to the use of extracts of Armerla pubigera and / or Saxifraga spathularls for the preparation of cosmetic and / or dermatological formulations to regenerate the dermal structure by fibroblastic stimulation.

Characterization of the extracts of Armerla Dubigera:

i) Determination of cellular stimulatory activity on a normal fibroblast line of human skin of Armerla pubigera extracts:

The capacity of cell stimulation on a normal fibroblast line of human skin (HSF) is measured according to the method of cell counting with Blue

5

Trypan [paduch al., Journal o (Elhnopharmacology; 2007, 110, 69-75].) As a negative control, HSF cells are suspended in 1 mL of a slightly enriched culture medium with 2.5% of Calf Serum Fetal (STF) As a positive control the HSF cells are suspended in 1 rnL of a complete culture medium with 10% Fetal Calf Serum (STF) For each contact time and for each concentration, the results are expressed in percentage of cellular gain in reference to the negative control determined according to the equation:

. 70

G. 1 1 (viable cells with ex / raelo) 100 ananCIQ ce u ar = x viable cells with e / negative control

\OR

Extracts of Armeria pubigera, obtained according to the extraction procedure included in the patenle [ES200901652 [, have a positive effect on cell stimulation on a normal fibroblast line of human skin.

Characterization of the extracts of Saxifraga spafhu / aris:

fifteen

i) Determination of the cellular stimulatory activity on a normal fibroblast line of human skin of the extracts of Saxifraga spathularis:

20 25

The capacity of cellular stimulation on a normal fibroblast line of human skin (HSF) is measured according to the cell concalation method with Trypan Blue [paduch al., Journal of Elhnopharmacology; 2007, 110, 69-75]. As a negative control, HSF cells are suspended in 1 ml of a slightly enriched culture medium with 2.5% Fetal Calf Serum (STF). As a positive control, HSF cells are suspended in 1 mL of a complete culture medium with 10% Fetal Calf Serum (STF). For each contact time and for each concentration, the results are expressed as a percentage of cellular gain in reference to the negative control determined according to the equation:

. , G. / / (viable cells with the extraclo) 100/0 ananclQ ce u ar = x viable cells with the negative control

30

The extracts of Saxifraga spalhularis, obtained according to the extraction procedure set out in the patent [ES200901653]. they have a positive effect on cell stimulation on a normal fibroblast line of human skin.

Another of the characteristic advantages of the invention will appear by reading the following examples, referring to the effects of the extracts of Armeria pubigera and Saxifraga spathularis on human skin fibroblasts, and the formulation examples, which are given illustratively of the

5 invention and should not be understood as limiting it.

EXAMPLES

Eiemolo 1:

Cellular stimulatory effect on a normal fibroblast line of human skin 10 of the extracts of Armerla pubigera:

An extract of Armeria pubigera, obtained according to the extraction procedure set out in the patent [ES200901652], presents an increase in the percentage of cellular gain shown in the following table 1:

% Cellular gain of the extract of Armeria pubigera (Ap2) Concentration Ap2 {} 1g / ml) 24h 48h 72h 160 <0.0% <0.0% <0.0% 80 <0.0% <0.0% <0.0% 40 <0.0% +55.4 % + 73.9% 20 <0.0% + 84.8% + 1130% 10 <0.0% + 43.9% + 1252%% Positive Control Cellular Gain (10% FCS) 24 h 48 h 72 h + 13.5 % + 104.2% +121, 5%

168 h <0.0% <0.0% + 126.9% + 182.1% + 216.0% 168 h +134 2%

15 Table 1: Percentage of cellular gain of the extract of Armeria pubigera Ap2. Example 2: Cellular stimulating effect on a normal fibroblast line of human skin

of the extracts of Saxifraga spathularis: An extract of Saxifraga spathularis obtained according to the extraction procedure set out in the patent [ES200901653], presents an increase in the percentage of cellular gain shown in the following table 2:

'' Cellular gain of the extract of Saxifraga spathu / aris (S52)

Concentration Ss2 () J.g / ml)

24h 48h 72h 168 h

160

<0.0% <0.0% <0.0% <0.0%

80

<0.0% + 30.2% <0.0% <0.0%

40

<0.0% + 137.5% + 108.2% + 117.8%

twenty

<0.0% + 172.9% + 183.4% + 302.5%

10

<0.0% + 193.8% +211, 1% + 365.9%

'' Cellular gain of the Positive Control (10% FCS)

24 h

48h 72h 168 h

+ 11.5%

+ 455% + 74.8% +151, 6%

Table 2: Percentage of cellular gain of the extract of Sax�fraga spathularis Ss2.

Example 3:

Formulation example according to the invention: Cream

5 Squalane 38% Olive wax 5% Armerla pubigera extract 1% Saxifraga spa / hularis extraclo 1% Emulsifier 10%

10 Glycerin 5% Purified water 40%

Example 4:

Formulation example according to the invention: Gel

15 Lauryl �ler Sodium Sulfalo 40% Extract from pubertal Armory 1% Extrachlore from Saxifraga spa / hularis 1% Coconut dielanolamide 5% Purified water 53% REVINDICATIONS

Claims (9)

one.

Cosmetic and / or dermatological formulations characterized because they contain at least one extract of Armena pubigera and / or Saxifraga spa / hu / aris.

2.

Cosmetic and / or dermatological formulations, according to claim 1. characterized by its ability to stimulate fibroblast.

3.

Cosmetic and / or dermatological formulations, according to claims 1 and 2, characterized in that they contain at least one extract of Armeria pubigera, which is characterized by having a positive effect on cell stimulation on a normal line Fibroblast of human skin.

Four.

Cosmetic and / or dermatological formulations, according to claims 1 to 3, characterized in that they contain at least one extract of pubertal Armory, which is characterized by an increase in the percentage of cellular gain of a normal fibrobl line Human skin esthetics, in reference to the negative control, between + 43.9% to + 216.0%, for a range of extract concentrations between 10.00 and 40.00 g / mL and after a while contact between 48 to 168 hours.

5.

Cosmetic and / or dermatological formulations, according to claims 1 and 2, characterized in that they contain, at least, an extract of Saxifraga spathularis, which is characterized by having a positive effect on cell stimulation on a normal line Fibroblast of human skin.

6.

Cosmetic and / or dermatological formulations according to claims 1, 2 and 5, characterized in that they contain at least one extract of Saxifraga spathularis, which is characterized by an increase in the percentage of cell gain of a line normal fibroblast of human skin, in reference to the negative control, between + 30.2% to + 365.9%, for a range of extract concentrations between 10.00 and 80.00 ~ glmL and after a contact time between 48 to 168 hours.

7.

Cosmetic and / or dermatological formulations, according to claims 1 to 6, characterized in that they also include one or more formulation agents

or additives such as dyes, preservatives, perfumes, oils, waxes, vitamins, UV filters, surfactants, emulsifiers, glycols, solvents and pH regulators. 8. Use of cosmetic and / or dermatological formulations, according to claims 1 to 7, to regenerate the dermal structure by fibroblastic stimulation. 9. Use of extracts of pubertarian Armory and / or Saxifraga spalhularis, according to claims 1 to 8, for the preparation of cosmetic formulations and / or Dermatology to regenerate the dermal structure through stimulation fibroblast