KR101776692B1 - Composiitn for skin whitening comprising extract of Osmanthus heterophylla - Google Patents

Abstract

The present invention relates to a composition for skin whitening comprising an extract of Artemisia sp. As an active ingredient. The extract of Artemisia sp. Has excellent effect of inhibiting the activity of tyrosinase, and thus can be usefully used as a composition for skin whitening. In addition, the extract of the articular tree has little toxicity and side effects to the human body, and thus can be safely applied to a cosmetic composition for skin whitening.

Description

TECHNICAL FIELD The present invention relates to a composition for skin whitening comprising extracts of osmanthus as an active ingredient and to a composition for skin whitening comprising extracts of Osmanthus heterophylla

The present invention relates to a composition for skin whitening comprising an extract of articular tree as an active ingredient, and more particularly to a cosmetic composition for skin whitening comprising, as an active ingredient, an extract of Artemisia majora, which has an effect of inhibiting tyrosinase activity will be.

In general, human eyes, hair and skin color are determined by the concentration and distribution of melanin in the skin, and also depending on the type of melanin. However, it is also affected by various environmental and physiological factors such as ultraviolet rays and stress. Melanin is a series of enzymes that starts from the conversion of melanocytes in the basal layer of the epidermis to tyrosinase-mediated tyrosinase in the melanosomes of melanocytes, the amino acid tyrosine, which is converted to dopa chromium Oxidation and non-enzymatic oxidation processes. There are ultraviolet rays as a typical influential factor for the production and synthesis of melanin. In addition, genetic factors, environmental factors, hormones, foods, medicines and other chemicals act. These melanin absorbs a certain amount of ultraviolet rays to protect the skin and maintain the body temperature. However, when the skin is exposed to severe stimuli and melanin is generated, freckles, senile spots, stains, brown or black spots, Or hyperpigmentation after inflammation due to dermatitis, and phototoxic reaction.

Recently, Oriental women prefer white and clean skin, and since they are used as an important criterion of beauty, development of a whitening agent for the treatment of skin pigmentation abnormality and to meet the desire for beauty is being actively carried out. Currently, in the development of a whitening agent for skin cosmetics, there is known a method of decolorizing by reducing the produced melanin pigment and a method of inhibiting the activity of tyrosinase, an enzyme that forms a melanin pigment. However, it has been known that whitening agents using tocopherol, vitamins and the like, which are used for reducing melanin pigment, have little discoloring effect on melanin pigment, and thus many studies on inhibitors that inhibit the production of melanin pigment have been conducted.

Conventionally, in order to improve skin whitening and skin hypercholesterolemia, an inhibitory activity against tyrosinase such as hydroquinone, ascorbic acid, kojic acid, glutathione and cysteine Have been used. However, hydroquinone exhibits a predetermined whitening effect, but it has a problem that skin irritation is serious, so that the blending amount is limited to a very small amount. Ascorbic acid is easy to be oxidized, and the cosmetic composition containing the same causes problems such as discoloration and deterioration. Has a disadvantage in that the manufacturing process of the cosmetic is complicated due to insufficient stability in the solution. In addition, thiol compounds such as glutathione and cysteine have not only unpleasant odors but also problems with transdermal absorption, and their glycosides and derivatives are also highly polar, making them difficult to use as a blending component of cosmetics. In addition, vitamin C is easily oxidized in an aqueous solution state and can not produce a sustained effect.

Accordingly, in recent years, compositions for skin whitening containing natural herbal extracts have been developed. As an example thereof, Korean Patent Registration No. 1428873 discloses a cosmetic composition for skin whitening comprising an extract of Paraliptera as an active ingredient. Korean Patent Registration No. 1415997 discloses a skin whitening cosmetic composition comprising a ginseng fruit extract as an active ingredient And Korean Patent Registration No. 1408019 discloses a cosmetic composition for whitening skin containing camellia sinica fruit extract.

On the other hand, Osmanthus heterophylla is an evergreen shrub belonging to the dyer of the dicotyledonous tree plant which grows on the beach. It has a little taste and is cool in nature. It protects the liver and kidney by pharmacological action, and treats arthritis and pertussis . However, the efficacy against the callus is not well known, and it is not known at all for the whitening use.

Accordingly, the inventors of the present invention confirmed that it is possible to use skin extract for skin whitening because it exhibits an effect of inhibiting the activity of tyrosinase, and thus completed the present invention.

It is an object of the present invention to provide an extract of articular tree having an excellent skin whitening effect by inhibiting the activity of tyrosinase.

It is another object of the present invention to provide a cosmetic composition comprising the extract as described above as an active ingredient.

In one aspect, the present invention provides a skin whitening composition comprising an extract of Artemisia as an active ingredient.

In the present invention, the oregano tree is an evergreen shrub belonging to the Asiatica family, including its natural, hybrid or variant.

In the present invention, the articular tree extract is a substance extracted from common leaves, branches or flowers, preferably flowers, by a conventional method, and has an activity of inhibiting the activity of tyrosinase.

The extract may be extracted using a conventional extraction solvent known in the art, and the extracted solution may be used in a liquid form or may be used by concentration and / or drying. The extraction solvent may be, for example, (a) water, (b) anhydrous or hydrated lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) Solvent, (d) acetone, (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol as the extraction solvent. Preferably, extraction is carried out using methanol, ethanol or butane. Depending on the extraction solvent, the degree of extraction and the degree of loss of the active ingredient of the extract may differ. Therefore, an appropriate extraction solvent should be selected and used. The extraction method is not particularly limited, and examples thereof include cold extraction, ultrasonic extraction, and reflux cooling extraction.

In addition, the arachidonicus extract can be obtained by a conventional purification process in addition to the extraction method using the above-mentioned extraction solvent. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, Through the fractionation, the extract of the articular tree can be obtained.

In one specific embodiment, the extract of articular tree of the present invention shows an effect of inhibiting the activity of tyrosinase.

Therefore, the composition comprising the extract of the present invention as an active ingredient can be usefully used for skin whitening.

The extract of articular tree contained in the skin whitening composition of the present invention may be contained in an amount of 0.00001 to 15.0% by weight, preferably 0.0001 to 10% by weight, more preferably 0.0001 to 5% by weight, based on the total weight of the whole composition .

As one specific use of the present invention, the composition of the present invention can be used as a cosmetic composition.

When the composition of the present invention is used as a cosmetic composition, it may further contain ingredients commonly used in cosmetic compositions in addition to the extract of Echinochloa as an active ingredient. For example, conventional adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavoring agents, and carriers.

The cosmetic composition of the present invention may be prepared in any formulations conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be prepared as a nutritional cream, a convergent lotion, a soft lotion, a lotion, an essence, a nutritional gel or a massage cream.

When the formulation of the cosmetic composition is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the cosmetic composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of spray, a mixture of chlorofluorohydrocarbons, Propane / butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition is an interface-active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

On the other hand, the extract of articular tree of the present invention is derived from plants and is harmless to the human body, has little toxicity and side effects, and can be safely used for prolonged use. In particular, it can be safely used in cosmetic compositions as described above.

The extract of articularis according to the present invention has an excellent effect of inhibiting the activity of tyrosinase, and thus can be usefully used as a composition for skin whitening. In addition, the extract of the articular tree has little toxicity and side effects to the human body, and thus can be safely applied to a cosmetic composition for skin whitening.

Hereinafter, the present invention will be described in more detail by way of examples and the like. It will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples and the like according to the gist of the present invention. will be.

Example 1: Preparation of extracts of articular tree

Flowers of Osmanthus heterophylla were collected from the southern part of Seoul National University (located in Baewoon - san, Gwangyang, Jeonnam). Next, 100% ethanol was added to 1 g of the flower, and the mixture was sonicated for 2 hours at 37 ° C in a water bath, and then concentrated under reduced pressure and lyophilized to prepare a powder.

Then, the powder was dissolved in distilled water again, and the solution was filtered with a 0.2 μm syringe filter to be diluted to a concentration of 100, 1000 or 2000 ppm. The diluted samples were used for subsequent experiments while blocking light and then cryopreserved.

Test Example 1: Measurement of inhibitory effect of tyrosinase activity

In order to evaluate the effect of the extract of the present invention on the tyrosinase activity, experiments were performed by modifying Xie's method known in the art.

Specifically, 50 μL of 0.1 M sodium phosphate buffer (pH 6.8) was added to a 96-well plate, and then 6 μL of the extract (100, 1,000 or 2,000 ppm) of the extract of the extract of the extract from the extract of Purkinje cells diluted with distilled water in Preparation Example 1 and 3 μL / 6 μL of tyrosinase was added. Then, 50 μL of 0.03% tyrosine solution and 38 μL of distilled water were further added, and the final volume was adjusted to 150 μL and reacted in a 5% CO ₂ incubator maintained at 37 ° C. for 10 minutes. After 10 minutes, the absorbance was measured at 475 nm using an ELISA reader (Epoch 2, Biotek, USA). As a negative control, distilled water was used in place of the extract of Echinochloa crus-galli. The inhibitory effect of tyrosinase activity was expressed as a percentage (%) inhibited compared to the control group not treated with oat extract. The results are shown in Table 1.

Extract 100 ppm 1,000 ppm 2,000 ppm Inhibition of tyrosinase activity (%) 47.7 ± 5.3 68.5 ± 2.9 83.2 ± 3.7

As shown in Table 1, it was confirmed that the arachidonicum extract inhibited the activity of tyrosinase, and the effect of inhibiting tyrosinase activity was increased as the treatment concentration was increased, indicating a concentration-dependent tendency.

Claims (3)

A cosmetic composition for skin whitening comprising an extract of Osmanthus heterophylla as an active ingredient.
The method according to claim 1,
The cosmetic composition for whitening skin according to claim 1, wherein the extract is from a flower of an orchard tree.
The method according to claim 1,
The cosmetic composition for whitening skin according to any one of claims 1 to 3, wherein the ointment extract is contained in an amount of 0.00001 to 15.0 wt% based on the total weight of the composition.