KR102371164B1 - Skin external composition for anti-aging containing germinated Glycine gracilis extract - Google Patents

Abstract

The present invention relates to an anti-aging composition for external application for skin containing a germinated flat bean extract as an active ingredient, and more specifically, it contains a flat bean extract germinated in a culture solution to which cinnamic acid is added, thereby having an excellent anti-aging effect. It relates to a composition for external application to the skin.
When the composition for external application for skin according to the present invention is used, the expression of dermal fibroblast growth factor in dermal fibroblasts synthesizing collagen is increased, and the expression and activity of collagenase is suppressed, thereby strengthening skin elasticity and reducing wrinkles It has excellent anti-aging effects such as improvement.

Description

Skin external composition for anti-aging containing germinated flat bean extract as an active ingredient {Skin external composition for anti-aging containing germinated Glycine gracilis extract}

The present invention relates to an anti-aging composition for external application for skin containing a germinated flat bean extract as an active ingredient, and more specifically, it contains a flat bean extract germinated in a culture solution to which cinnamic acid is added, thereby having an excellent anti-aging effect. It relates to a composition for external application to the skin.

The skin is the human body's primary defense barrier, and it protects various organs from external environmental stimuli such as temperature and humidity changes, ultraviolet rays and pollutants, and plays an important role in maintaining body homeostasis such as body temperature regulation. However, excessive physical and chemical stimuli, stress, and nutritional deficiencies from the outside reduce the normal function of the skin and accelerate skin aging such as loss of elasticity, keratinization, and wrinkle formation.

In order to prevent this phenomenon and maintain healthy and elastic skin, cosmetics containing physiologically active substances obtained from various plants and microorganisms are manufactured to maintain the unique function of the skin and activate skin cells to effectively prevent skin aging. Efforts have been made to contain it. However, most of the existing cosmetic raw materials have various problems such as insufficient efficacy or causing skin side effects.

The flat bean ( Glycine gracilis ) used in the present invention comes from the etymology that the shape is flat, and it is variously called as naeptegi bean, naphteggi bean (cheongsong), napdreekong (Yeongil), flat bean (Gyeonggi-do), and the like. Unlike soybeans, which are the most common soybeans, they are oval and have a flat and slender shape. If you plant lead-tegi beans before Chobok, the vines will stretch out a lot. The leaves of leopard bean are rather narrow and small, and have blue flowers. When the lead bean sprouts are grown as bean sprouts, they are characterized by fast growth, a slender head, small and soft, and good taste.

Paying attention to the feature that soybeans are rich in antioxidants and anti-aging ingredients, the present inventors disclosed in Korean Patent Application Publication No. 2013-0107123, a composition containing an extract of ungerminated soybean (flat bean) It has been found to have excellent antioxidant, anti-aging, whitening, or moisturizing effects.

As an extension of the research related to the anti-aging effect of the flat bean extract, the present inventors made intensive efforts to further enhance the skin wrinkle improvement effect and the skin elasticity enhancing effect of the flat bean extract. In the case of the flat bean extract germinated in the culture medium, the expression of the dermal fibroblast growth factor is further enhanced and the expression of the collagen degrading enzyme is further suppressed, so that the expression of the collagen degrading enzyme is more excellent than the case of the ungerminated flat bean or the flat bean extract germinated without cinnamic acid. It was confirmed that the inhibitory effect was exhibited, and the present invention was completed.

Korea Patent Publication No. 2013-0107123 (2013.10.01)

It is an object of the present invention to provide a composition for external application for skin that exhibits excellent anti-aging effect by containing as an active ingredient an extract of flat beans germinated in a culture medium to which cinnamic acid is added.

In order to achieve the above object, the present invention is an anti-aging composition for external application for skin containing a germinated flat bean extract as an active ingredient, wherein the germinated flat bean extract is obtained from germinated flat bean extract in a culture medium to which cinnamic acid is added, A composition for external application to the skin is provided.

In addition, the present invention is a composition for external application for skin wrinkle improvement containing a germinated flat bean extract as an active ingredient, wherein the germinated flat bean extract is obtained from flat beans germinated in a culture solution to which cinnamic acid is added. provides

In addition, the present invention is a composition for skin external application for improving skin elasticity containing a germinated flat bean extract as an active ingredient, wherein the germinated flat bean extract is obtained from flat beans germinated in a culture solution to which cinnamic acid is added. provides

When the composition for external application for skin according to the present invention is used, the expression of dermal fibroblast growth factor in dermal fibroblasts synthesizing collagen is increased, and the expression and activity of collagenase is suppressed, thereby strengthening skin elasticity and reducing wrinkles It has excellent anti-aging effects such as improvement.

1 shows the results of high performance liquid chromatography (HPLC) component analysis for each of the non-germinated flat bean extract (Comparative Example 1), the germinated flat bean extract (Comparative Example 2), and the cinnamic acid germinated flat bean extract (Example 1). will be compared

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is those well known and commonly used in the art.

As used in the present invention, "sprouted flat bean extract with cinnamic acid" means a material extracted from flat beans germinated in a culture medium to which cinnamic acid is added, and "germinated flat bean extract" without the prefix "cinnamic acid" means that cinnamic acid is not added. It refers to a material extracted from flat beans germinated in a conventional culture medium.

Also, in the present invention, "collagen degrading enzyme" and "collagenase" are used as the same meaning.

In an embodiment of the present invention, the expression of dermal fibroblast growth factor and collagenase in an extract obtained from germinated flat beans in an aqueous cinnamic acid solution, an extract obtained from germinated flat beans without an aqueous cinnamic acid solution, and an extract obtained from ungerminated flat beans As a result of measuring and comparing each of these, the flat bean extract germinated in an aqueous cinnamic acid solution further promotes the expression of dermal fibroblast growth factor and further inhibits the expression and activity of collagen degrading enzyme, thereby providing excellent skin wrinkle improvement effect and skin elasticity It was found that the enhancement effect was shown.

Accordingly, in one aspect, the present invention is an anti-aging composition for external application for skin containing a germinated flat bean extract as an active ingredient, wherein the germinated flat bean extract is obtained from flat beans germinated in a culture medium to which cinnamic acid is added, characterized in that It relates to a composition for external use.

In addition, in another aspect, the present invention is a composition for external application for skin wrinkle improvement or skin elasticity improvement containing a germinated flat bean extract as an active ingredient, wherein the germinated flat bean extract is obtained from germinated flat beans in a culture medium to which cinnamic acid is added. It relates to a composition for external application for skin, characterized in that obtained.

In the present invention, the composition promotes the expression of dermal fibroblast growth factor that synthesizes collagen, which is a major component of skin tissue, or inhibits the expression of collagen-degrading enzyme, thereby providing anti-aging effects, particularly improving skin elasticity and improving skin elasticity. It may be characterized in that it exhibits a wrinkle-improving effect.

The scientific name of the flat bean used in the present invention is Glycine gracilis , and in Korean, it is variously called as lead bean, napdekong, naphtaegi, naphdreong, flat bean, and the like. "Germination" refers to a phenomenon in which buds formed in seeds, spores, pollen, branches or roots of plants, etc. occur or start to grow. In the present invention, flat beans when sprouts grow to about 20-60 mm are defined as "germinating flat beans".

In addition, cinnamic acid used for germinating flat beans in the present invention is also called cinnamic acid, broileric acid, β-phenylacrylic acid, and the like, and is one of representative aromatic unsaturated carboxylic acids. It is contained in a lot of plant extracts such as broiler oil (cinnamon oil), Peru balsam, and bovine frankincense oil, and can be artificially synthesized. In the present invention, cinnamic acid added to the culture medium has a function similar to that of a plant hormone, and it is assumed that it induces the operation of a signaling system in the plant to produce more anti-aging-related substances. It is most preferable to add cinnamic acid to the culture medium, but it may be used instead of jasmonic acid or p - coumaric acid.

In the present invention, the culture solution to which cinnamon acid is added used for germination of flat beans may refer to an aqueous cinnamic acid solution, and may be one in which an auxiliary component that can be normally added to the culture solution is further added to the cinnamic acid aqueous solution. The concentration of cinnamic acid in the culture medium to which cinnamic acid is added may be preferably 0.50 to 1.00 mM, and more preferably 0.75 mM.

In the present invention, the germinated flat bean extract may be characterized in that it is prepared by a process comprising the following steps.

a) germinating flat beans using a culture solution to which cinnamic acid is added;

b) drying and then pulverizing the flat beans germinated in step a);

c) mixing the flat bean powder powdered in step b) with a polar solvent and then boiling the powder for primary extraction;

d) filtering the primary extract obtained in step c);

e) secondary extraction of the extract filtered in step d) with a low-polarity or non-polar organic solvent; and

f) concentrating the final extract obtained in step e) under reduced pressure.

The cinnamic acid germinated flat bean extract contained in the composition of the present invention is particularly prepared by sequential extraction by changing the polarity, and the cinnamic acid germinated flat bean extract is used in anhydrous or hydrated lower alcohol (1 to 3 carbon atoms), polar solvent such as acetone. , preferably after primary extraction with ethanol, re-extraction (secondary extraction) with a low polar or non-polar solvent such as ethyl acetate, diethyl ether, benzene, chloroform, and hexane, preferably ethyl acetate to maximize the content of the active ingredient, By removing impurities, it is possible to prepare an extract with improved stability and maximized efficacy. Then, it can be used by adding it to external preparations for skin, for example, from softening lotion using solubilization technology to nutrient lotion applying emulsification technology, cream, essence, etc.

In addition, by performing the filtration process twice between the primary extraction and the secondary extraction using a polar solvent, the extract can be efficiently purified. The primary filtration is preferably filtered through a 200-300 mesh filter cloth, and the primary filtered filtrate is aged at 4-15° C. for several days, followed by secondary filtration with filter paper.

In addition, in the present invention, the cinnamic acid germinated flat bean extract may be characterized in that it is contained in an amount of 0.01 to 10% by weight based on the total weight of the composition. If the content of the extract is less than 0.01% by weight, it is difficult to expect the effect, and if it exceeds 10% by weight, the increase in the effect does not appear clearly anymore.

When the composition for external application for skin according to the present invention is formulated in the form of a cosmetic, softening lotion, astringent lotion, nutrient lotion, eye cream, nourishing cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack, etc. It may be formulated in the form of, and the formulation is not particularly limited. In addition, the composition according to the present invention is a fatty substance, an organic solvent, a solubilizer, a thickening agent, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or non Ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics; It may contain adjuvants commonly used in the field of cosmetics or dermatology. The adjuvant is introduced in an amount generally used in the field of cosmetology or dermatology. In addition, the composition of the present invention may contain a skin absorption promoting material to increase the skin improvement effect.

The composition for external application for skin according to the present invention may include other ingredients other than the above substances within a range that does not impair the main effect, preferably other ingredients capable of giving a synergistic effect to the main effect. In addition, the composition for external application for skin according to the present invention may further include a moisturizer, an emollient, a UV absorber, a preservative, a disinfectant, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cooling agent or a limiting agent. The blending amount of the component can be easily selected by those skilled in the art within the range that does not impair the purpose and effect of the present invention, and the blending amount may be 0.01 to 5 wt%, specifically 0.01 to 3 wt%, based on the total weight of the composition there is.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

[Example 1] Preparation of cinnamic acid germinated flat bean extract

Using water to which cinnamic acid was added at a concentration of 0.75 mM, 100 g of flat beans were germinated for 48 hours in a germination machine maintained at a temperature of 25°C. The average length of flat bean sprouts germinated for 48 hours was 25 mm.

Then, the germinated flat beans were washed with purified water, dried, and pulverized to obtain 100 g of cinnamic acid germinated flat bean powder. 100 g of cinnamic acid germinated flat bean powder was added to 1 liter of 70% ethanol aqueous solution, boiled for 12 hours in an extractor equipped with a cooling condenser, and extracted, followed by filtration with a 300 mesh filter cloth. The resulting filtrate was aged at 4-15° C. for 7 days, and then filtered again with Whatman No. 2 filter paper. The extract thus obtained was put into a 3-liter separatory funnel, 1 liter of ethyl acetate was added, and the mixture was shaken to mix well, and when it was completely separated into two layers, the upper layer (ethyl acetate layer) was taken. The lower layer (water layer) was extracted by repeating the above process twice more using a separatory funnel. The upper layers separated in each extraction process were combined, concentrated under reduced pressure at 50° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain an extract of germinated flat bean cinnamon (dry weight 13.54 g).

[Comparative Example 1] Preparation of ungerminated flat bean extract

The ungerminated flat beans were washed with purified water, dried and then pulverized to obtain 100 g of flat soybean flour. 100 g of flat bean powder was added to 1 liter of 70% ethanol aqueous solution, boiled for 12 hours in an extractor equipped with a cooling condenser, and extracted, and then filtered through a 300 mesh filter cloth. The resulting filtrate was aged at 4-15° C. for 7 days, and then filtered again with Whatman No. 2 filter paper. The extract thus obtained was put into a 3-liter separatory funnel, 1 liter of ethyl acetate was added, and the mixture was shaken to mix well, and when it was completely separated into two layers, the upper layer (ethyl acetate layer) was taken. The lower layer (water layer) was extracted by repeating the above process twice more using a separatory funnel. The upper layers separated in each extraction process were combined, concentrated under reduced pressure at 50° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain an ungerminated flat bean extract (dry weight 15.18 g).

[Comparative Example 2] Preparation of germinated flat bean extract

First, using distilled water, 100 g of flat beans were germinated for 48 hours in a germination machine maintained at a temperature of 25°C. The average length of flat bean sprouts germinated for 48 hours was 55 mm. The reason that the average length of the shoots is longer than that of Example 1, which was germinated using an aqueous solution of cinnamic acid, is thought to be because in the case of soybeans, cinnamic acid inhibits the elongation of the stem length (http://journals.plos.org/plosone/article). ?id=10.1371/journal.pone.0069105 Reference).

Then, the germinated flat beans were washed with purified water, dried, and pulverized to obtain 100 g of germinated flat beans. 100 g of germinated flat bean powder was added to 1 liter of 70% ethanol aqueous solution, boiled for 12 hours in an extractor equipped with a cooling condenser, and extracted, followed by filtration with a 300 mesh filter cloth. The resulting filtrate was aged at 4-15° C. for 7 days, and then filtered again with Whatman No. 2 filter paper. The extract thus obtained was put into a 3-liter separatory funnel, 1 liter of ethyl acetate was added, and the mixture was shaken to mix well, and when it was completely separated into two layers, the upper layer (ethyl acetate layer) was taken. The lower layer (water layer) was extracted by repeating the above process twice more using a separatory funnel. The upper layers separated in each extraction process were combined, concentrated under reduced pressure at 50° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a germinated flat bean extract (dry weight 14.16 g).

[Test Example 1] Comparative analysis of extract components

Each component of the cinnamic acid germinated flat bean extract of Example 1, the ungerminated flat bean extract of Comparative Example 1, and the germinated flat bean extract of Comparative Example 2 was analyzed. Each of the extracts was dissolved in 70% ethanol to make a 10,000 ppm solution, and component analysis (Waters, 2996 PDA detector) was performed using HPLC (Waters, 2695 model). As a stationary phase, a Mightysil RP-18 GP 250-4.6 (5 μm) column of Kanto Chemical was used, and the composition ratio as shown in Table 1 below was used for the mobile phase.

time (minutes) A: water B: acetonitrile 0 95 5 35 65 35 45 5 95 50 95 5 55 95 5

As a result, as shown in Figure 1, in the cinnamic acid germinated flat bean extract, a new peak that was not observed in the non-germinated flat bean extract and the germinated flat bean extract was observed, confirming the presence of a novel component. there was. Therefore, due to this component, it could be predicted that the cinnamic acid germinated flat bean extract would have superior anti-aging effects compared to the ungerminated flat bean extract and the germinated flat bean extract.

[Test Example 2] Test for measuring dermal fibroblast growth factor expression

The dermal fibroblast growth factor expression level of each of the cinnamic acid germinated flat bean extract of Example 1, the ungerminated flat bean extract of Comparative Example 1, and the germinated flat bean extract of Comparative Example 3, 2.5% by weight of fetal bovine serum It was measured compared to the contained DMEM (Dulbecco's Modified Eagle's Media) medium.

5,000 cells/well of human fibroblasts (Cascade Biologics, Portland, OR, USA) in a 96-well microtiter plate containing DMEM medium containing 2.5% by weight of fetal bovine serum. ), and incubated until about 90% growth. After culturing in serum-free DMEM medium for 24 hours, the cinnamic acid germinated flat bean extract of Example 1, the ungerminated flat bean extract of Comparative Example 1, and the germinated flat bean of Comparative Example 2 dissolved in serum-free DMEM medium. Each extract was taken by 50ppm and treated for 24 hours. For the positive control group, the serum-free DMEM medium was replaced with a DMEM medium containing 2.5% by weight of fetal bovine serum and cultured for 24 hours, and then the cell culture medium was collected.

Then, the expression level of the dermal fibroblast growth factor in the cell culture medium collected using a dermal fibroblast growth factor measuring instrument (R&D System, USA) was measured. First, the collected cell culture solution was placed in a 96-well plate coated with the primary dermal fibroblast growth factor antibody uniformly, and the antigen-antibody reaction was performed in a constant temperature bath (36° C.) for 3 hours.

After 4 hours, the chromophore-conjugated secondary dermal fibroblast growth factor antibody was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, add a color-generating substance to develop color at room temperature for 15 minutes, and then add 1M sulfuric acid to stop the reaction (color development), the color of the reaction solution is yellow. was confirmed.

The absorbance of the 96-well plate with blue color was measured at 450 nm using a light absorber, and the expression level of dermal fibroblast growth factor was calculated by Equation 1 below. At this time, the reaction absorbance of the cell culture solution collected from the group not treated with the composition was used as a control. That is, the expression level of dermal fibroblast growth factor in the untreated group was set to 100, and in contrast, the expression level of dermal fibroblast growth factor in the group treated with the test substance was obtained, and the results are shown in Table 2 below. shown in

test substance Dermal fibroblast growth factor expression level (%) untreated group 100 2.5% by weight of fetal bovine serum
DMEM medium containing 196 Cinnamon-acid germinated flat bean extract (Example 1) 189 Ungerminated flat bean extract (Comparative Example 1) 124 Germinated flat bean extract (Comparative Example 2) 129

As shown in Table 2 above, the cinnamic acid germinated flat bean extract of Example 1 was better than the ungerminated flat bean extract of Comparative Example 1 or the germinated flat bean extract of Comparative Example 2 in vitro . was found to more effectively increase the expression of Therefore, it can be confirmed that the extract according to the present invention or a composition containing the same exhibits an excellent anti-aging effect, that is, an excellent skin wrinkle improvement effect and skin elasticity improvement effect.

[Test Example 3] Anti-aging ability test

The cinnamic acid germinated flat bean extract of Example 1, the ungerminated flat bean extract of Comparative Example 1, and the germinated flat bean extract of Comparative Example 2 were each measured for their ability to inhibit collagenase production by comparing them with tocopherol and EGCG. . Tocopherol and EGCG are substances known to have a function of preventing skin aging by regenerating epidermal cells of the skin.

Human fibroblasts (Cascade Biologics) (Portland, OR, USA) were placed in a 96-well microtiter plate containing DMEM (Dulbecco's Modified Eagle's Media) medium containing 2.5% by weight of fetal bovine serum. It was added so that it became 5,000 cells/well, and cultured until about 90% growth. After culturing in serum-free DMEM medium for 24 hours, the cinnamic acid germinated flat bean extract of Example 1, the ungerminated flat bean extract of Comparative Example 1, and the germinated flat bean of Comparative Example 2 dissolved in serum-free DMEM medium. The extracts were each treated to a concentration of 50 ppm, and tocopherol and EGCG (Sigma Aldrich) were each treated at a concentration of 50 μM for 24 hours, and then the cell culture solution was collected.

Then, using a collagenase measuring instrument (Amersham Pharma Corporation, USA), the collagenase production level of the collected cell culture was measured. First, the collected cell culture solution was placed in a 96-well plate uniformly coated with the primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat (36° C.) for 3 hours.

After 3 hours, the chromophore-conjugated secondary collagen antibody was placed in a 96-well plate and reacted for 15 minutes again. After 15 minutes, add a color-generating substance to develop color at room temperature for 15 minutes, and then add 1M sulfuric acid to stop the reaction (color development), the color of the reaction solution is yellow. was confirmed.

Absorbance of a 96-well plate having a yellow color was measured at 405 nm using an absorber, and the degree of collagenase synthesis was calculated by Equation 2 below. At this time, the reaction absorbance of the cell culture solution collected from the group not treated with the composition was used as a control. That is, the expression level of collagenase in the untreated group was set to 100, and in contrast to this, the expression level of collagenase in the group treated with the test substance was obtained, and the results are shown in Table 3 below.

test substance Collagenase expression level (%) untreated group 100 tocopherol 75 EGCG 61 Cinnamon-acid germinated flat bean extract (Example 1) 58 Ungerminated flat bean extract (Comparative Example 1) 79 Germinated flat bean extract (Comparative Example 2) 76

As shown in Table 3 above, the expression of collagenase in the cinnamic acid germinated flat bean extract of Example 1 in vitro than the ungerminated flat bean extract of Comparative Example 1 or the germinated flat bean extract of Comparative Example 2 It was found that the ability to inhibit the expression of collagenase was superior to that of tocopherol and EGCG, which are known as anti-aging substances. Therefore, it can be confirmed that the extract according to the present invention or a composition containing the same exhibits an excellent anti-aging effect, particularly an excellent skin wrinkle improvement effect and skin elasticity improvement effect.

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereby. something to do. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.

In addition, the formulation examples of the composition for external application for skin including the composition of the present invention will be described in more detail below, but it will be clear that the scope of the present invention is not limited thereby.

[Formulation Example 1] Nutrient lotion

Nutrient lotion was prepared by a conventional method according to the composition shown in Table 4 below (unit: wt%).

ingredient content Purified water remaining amount glycerin 8.0 butylene glycol 4.0 Hyaluronic Acid Extract 5.0 beta glucan 7.0 Carbomer 0.1 Cinnamon-acid germinated flat bean extract (Example 1) 0.05 Caprylic/Capric Triglycerides 8.0 squalane 5.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 cetearyl alcohol 1.0 triethanolamine 0.1

[Formulation Example 2] Nourishing cream

Nutrient creams were prepared in a conventional manner according to the composition shown in Table 5 below (unit: wt%).

ingredient content Purified water remaining amount glycerin 3.0 butylene glycol 3.0 liquid paraffin 7.0 beta glucan 7.0 Carbomer 0.1 Cinnamon-acid germinated flat bean extract (Example 1) 3.0 Caprylic/Capric Triglycerides 3.0 squalane 5.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 Polysorbate 60 1.2 triethanolamine 0.1

[Formulation Example 3] Massage Cream

A massage cream was prepared in a conventional manner according to the composition shown in Table 6 below (unit: wt%).

ingredient content Purified water remaining amount glycerin 8.0 butylene glycol 4.0 liquid paraffin 45.0 beta glucan 7.0 Carbomer 0.1 Cinnamon-acid germinated flat bean extract (Example 1) 1.0 Caprylic/Capric Triglycerides 3.0 beeswax 4.0 cetearyl glucoside 1.5 Sorbitan Sesquioleate 0.9 Vaseline 3.0 paraffin 1.5

[Formulation Example 4] Pack

A pack was prepared in a conventional manner according to the composition shown in Table 7 (unit: wt%).

ingredient content Purified water remaining amount glycerin 4.0 polyvinyl alcohol 15.0 Hyaluronic Acid Extract 5.0 beta glucan 7.0 allantoin 0.1 Cinnamon-acid germinated flat bean extract (Example 1) 0.5 nonylphenyl ether 0.4 Polysorbate 60 1.2 ethanol 6.0

[Formulation Example 5] Ointment for external skin preparations

An ointment was prepared in a conventional manner according to the composition shown in Table 8 (unit: wt%).

ingredient content Purified water remaining amount glycerin 8.0 butylene glycol 4.0 liquid paraffin 15.0 beta glucan 7.0 Carbomer 0.1 Cinnamon Germination Flat Bean Extract (Example 1) 1.0 Caprylic/Capric Triglycerides 3.0 squalane 1.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 cetearyl alcohol 1.0 beeswax 4.0

Claims (10)

As an anti-aging skin external composition containing germinated flat bean extract as an active ingredient,
The germinated flat bean extract is obtained from germinated flat beans in a culture medium to which cinnamic acid is added,
The cinnamic acid concentration of the culture solution to which the cinnamic acid is added is 0.50 to 1.00 mM, characterized in that the composition for external application for skin. A composition for external application for skin wrinkle improvement containing germinated flat bean extract as an active ingredient, the composition comprising:
The germinated flat bean extract is obtained from germinated flat beans in a culture medium to which cinnamic acid is added,
The cinnamic acid concentration of the culture solution to which the cinnamic acid is added is 0.50 to 1.00 mM, characterized in that the composition for external application for skin. A composition for external application for skin for improving skin elasticity containing germinated flat bean extract as an active ingredient, the composition comprising:
The germinated flat bean extract is obtained from germinated flat beans in a culture medium to which cinnamic acid is added,
The cinnamic acid concentration of the culture solution to which the cinnamic acid is added is 0.50 to 1.00 mM, characterized in that the composition for external application for skin. The method according to any one of claims 1 to 3, wherein the germinated flat bean extract is
a) germinating flat beans using a culture solution to which cinnamic acid is added;
b) drying the flat beans germinated in step a) and then pulverizing them;
c) mixing the flat bean powder powdered in step b) with a polar solvent and then boiling the powder for primary extraction;
d) filtering the primary extract obtained in step c);
e) secondary extraction of the extract filtered in step d) with a low- or non-polar organic solvent; and
f) concentrating the final extract obtained in step e) under reduced pressure;
A composition for external application for skin, characterized in that it is prepared by a process comprising a. 4. The method according to any one of claims 1 to 3, wherein the The composition for external application for skin, characterized in that the concentration of cinnamic acid is 0.75mM. The composition for external application for skin according to claim 4, wherein the polar solvent in step c) is an alcohol having 1 to 3 carbon atoms, and the low polar or non-polar organic solvent in step e) is ethyl acetate. [Claim 5] The composition for external application for skin according to claim 4, wherein the filtration in step d) is first filtered with a filter cloth, the first filtered filtrate is aged at 4-15° C., and second filtered with a filter paper. . The composition for external application for skin according to any one of claims 1 to 3, wherein the germinated flat bean extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the composition. The composition for external application for skin according to any one of claims 1 to 3, wherein the composition promotes the expression of dermal fibroblast growth factor. The composition for external application for skin according to any one of claims 1 to 3, wherein the composition inhibits the expression of a collagen-degrading enzyme.

Images