KR102223617B1 - Cosmetic composition containing Clitoria ternatea extract, Buddleja Davidii extract and Centaurea cyanus extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mixed fermented extract of Clitoria ternatea, Buddleja davidii, and Centaurea cyanus as an active component. The mixed extract of Clitoria ternatea, Buddleja davidii, and Centaurea cyanus exhibits excellent effects of alleviating skin wrinkles, improving elasticity, skin moisturizing, reliving skin inflammation, and skin regeneration, thereby being able to be usefully used as anti-aging cosmetics.

Description

Cosmetic composition containing Clitoria ternatea extract, Buddleja Davidii extract and Centaurea cyanus extract}

The present invention relates to a cosmetic composition containing an extract of a butterfly bean flower, a cordelia and a cornflower flower as an active ingredient, and specifically, a mixed ferment extract of a butterfly bean flower, a cordia flower and a cornflower flower, showing excellent skin improvement effect. It relates to a cosmetic composition.

Due to the aging of the population and the improvement of income levels, interest in health is increasing, and accordingly, industries to improve aging-related diseases are developing. These industries are called anti-aging industries, and the anti-aging industries include pharmaceuticals, medical devices, food, and cosmetics industries. Among them, as interest in appearance increases, interest in anti-aging cosmetics industry is increasing. Anti-aging cosmetics have the effect of protecting the skin from external stimuli and preventing aging through the functions of improving wrinkles, blocking UV rays, increasing skin moisturization, and preventing and alleviating inflammation. These cosmetics not only enhance the attractiveness of human appearance, but also improve skin health, overcome the aging phenomenon caused by aging and satisfy human aesthetic needs through skin improvement.

The inventors of the present invention were studying natural products with skin-improving effect, and researched the activity by preparing a mixed extract of butterfly bean flower, bedelaria and cornflower flower, and in this case, the fermented extract of butterfly bean flower, bedelaria and cornflower flower mixed The present invention was completed by discovering that it can be usefully used as a cosmetic material by showing an excellent skin improvement effect.

(0001) Korean Patent Registration No. 10-1727537 (2017.05.02.) (0002) Korean Patent Application Publication No. 10-2010-0031068 (2010.03.19)

An object of the present invention is to provide a cosmetic composition exhibiting excellent skin improvement effect by containing a fermented extract of butterfly bean flower, bedelaria and cornflower flower mixed as an active ingredient.

In addition, another object of the present invention is to provide a method for preparing a fermented extract of a mixed fermented extract of a butterfly bean flower, a budelia and a cornflower flower showing an excellent skin improvement effect.

In order to achieve the above object, according to the present invention, there is provided a cosmetic composition for skin improvement containing a fermented extract of a butterfly bean flower, Bdeliia and cornflower flower mixed fermentation. As an active ingredient, the mixed fermented extract of Bdelia and cornflower flowers is contained in an amount of 0.01 to 90% by weight based on the total weight of the cosmetic composition.

The mixed fermentation extract is characterized in that it is fermented with Saccharomyces cerevisiae from a mixed extract of butterfly bean flower, Bdeliia and cornflower flower. In this case, the mixed extract is characterized in that it is extracted by mixing the butterfly bean flower, the budelia, and the cornflower flower in a weight ratio of 1 to 2: 1 to 2: 1 to 2, respectively.

The cosmetic composition is characterized in that for improving skin wrinkles and elasticity, for moisturizing the skin, for relieving skin inflammation or for skin regeneration.

According to the present invention in order to achieve the above other object, (A) preparing a mixture by mixing a butterfly bean flower, a budelia and a cornflower flower in a weight ratio of 1 to 2: 1 to 2: 1 to 2, respectively;

(B) preparing a mixed extract by adding 5 to 10 times the weight of the solvent to the mixture and extracting at 80 to 100°C for 8 to 10 hours;

(C) fermenting the mixed extract with Saccharomyces cerevisiae for 3 to 5 days at 22 to 28° C. after inoculation; And

(D) After sterilization, there is provided a method for preparing a fermented extract of a mixed fermented extract of butterfly bean flower, bradelia and cornflower flower comprising the step of filtration and concentration.

The solvent is at least one selected from the group consisting of water, alcohol, ethyl acetate, glycerin, ethylene glycol, propylene glycol, and butylene glycol.

The mixed fermented extract of butterfly bean flower, budelia and cornflower flower according to the present invention exhibits excellent skin wrinkle improvement effect, skin elasticity improvement effect, skin moisturizing effect, skin inflammation alleviation effect and skin regeneration effect by its synergistic effect. It can be usefully used as a cosmetic.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a cosmetic composition containing as an active ingredient a fermented extract of butterfly bean flower, budelia and cornflower flower mixed.

Butterfly bean (Clitoria ternatea) used in the present invention is known by the name of butterfly pea (Butterfly pea). It is a plant that grows and cultivates itself because of its strong vitality, and is used for health in Southeast Asia and China. Butterfly bean flower contains an antioxidant called proanthocyanidin, which helps improve skin damage and skin aging, and is known to help with various inflammatory diseases because it is rich in flavonoids.

Buddleja Davidii is a plant of genus gentian and budleya genus in the plywood group of dicotyledons, and is called Butterfly Bush. Its shape, scent, and color are so fascinating to butterflies of all species that it is called Butterfly Magnet. It is known to relieve skin itching, heal wounds, and detoxify. In the present invention, it is possible to use an outpost, flowers, leaves, etc. of Bradelia in the preparation of the extract, and preferably, an outpost is used.

Cornflower (Centaurea cyanus) is a plant that has been closely used in people's lives from ancient times. It has excellent skin regeneration and antioxidant effects, and is excellent in making the skin healthy and smooth. It is also known to be effective in relieving and calming inflammation by preventing inflammation from external factors.

The present invention relates to a skin-improving cosmetic composition containing a mixed fermented extract of butterfly bean flower, budelia and cornflower flower as an active ingredient. It was confirmed that the mixed extracts of butterfly bean flower, budelia and cornflower flower themselves showed excellent skin improvement activity, but the mixed fermented extracts thereof showed more excellent skin improvement activity.

The mixed fermentation extract is prepared by mixing and extracting butterfly bean flowers, budelia and cornflower flowers, or by fermenting at the same time as extraction. As the fermentation strain, yeast, lactic acid bacteria, or a mixture thereof can be used, and when a ferment extract is prepared using yeast (Saccharomyces cerevisiae), it exhibits more excellent skin wrinkles and elasticity improvement, skin moisturization, skin inflammation relief and skin regeneration activity. Therefore, it is more preferable.

According to an embodiment of the present invention, the mixed fermentation extract is prepared by the following method.

(A) preparing a mixture by mixing a butterfly bean flower, a budelia, and a cornflower flower in a weight ratio of 1 to 2: 1 to 2: 1 to 2, respectively; (B) preparing a mixed extract by adding 5 to 10 times the weight of the solvent to the mixture and extracting at 80 to 100°C for 8 to 10 hours; (C) fermenting the mixture extract with Saccharomyces cerevisiae for 3 to 5 days at 22 to 28° C. after inoculation; And (D) filtering and concentrating after sterilization.

First, a mixture is prepared by mixing a butterfly bean flower, a budelia, and a cornflower flower in a dry weight ratio of 1 to 2: 1 to 2: 1 to 2, respectively. At this time, it is possible to use an outpost, flowers, leaves, etc. of Bradelia, preferably an outpost.

Then, 5 to 10 times the weight of the solvent is added to the mixture and extracted at 80 to 100°C for 8 to 10 hours to prepare a mixed extract. At this time, as the solvent, at least one solvent selected from the group consisting of water, alcohol having 2 to 5 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, and butylene glycol is used, and a mixed solvent of water and ethanol is used. In this case, it is preferable because the yield and skin improvement activity are more excellent.

After preparing the mixture, Saccharomyces cerevisiae as a fermentation strain was inoculated and fermented at 22 to 28° C. for 3 to 5 days. Then, after sterilization, it is filtered and concentrated to prepare a mixed fermented extract of butterfly bean flower, budelia and cornflower flower.

At this time, in the case of extracting by mixing butterfly bean flower, budelia, and cornflower in a weight ratio of 2:1:2, and fermenting it with Saccharomyces cerevisiae, the yield, wrinkle improvement, and elasticity , Moisturizing, inflammation alleviation and skin regeneration effect showed the best effect.

The mixed fermentation extract prepared in this way is more excellent than the mixed extract of butterfly bean flower, brolia, and cornflower flower, fermented lactic acid bacteria extract, and fermented acetic acid by enhancing the synergistic effect of mixing and fermentation effect. Expression increasing effect (Test Example 1), wrinkle-related factor generation increasing effect (Test Example 2), elasticity-related factor expression increasing effect (Test Example 3), moisturizing-related factor expression increasing effect (Test Example 4), skin inflammation alleviating effect ( Test Example 5) and skin cell regeneration effect (Test Example 6) were shown.

In the cosmetic composition of the present invention, the mixed fermented extract of butterfly bean flower, budelia and cornflower flower is contained in an amount of 0.01 to 90% by weight based on the total weight of the cosmetic composition.

The cosmetic composition containing the mixed fermented extract of butterfly bean flower, budelia and cornflower flower of the present invention can be prepared in any commonly prepared formulation, examples of which include lotion, cream, essence, cleansing foam, cleansing water, pack , Body lotion, body oil, body gel, shampoo, conditioner, hair conditioner, hair gel, foundation, lipstick, mascara, makeup base, mask sheet, sun products, and soap.

[Example]

Hereinafter, the present invention will be described in detail through the following examples and test examples, but the present invention is not limited by these examples.

Example 1: Preparation of mixed fermented extract of butterfly bean flower, Bdeliia and cornflower flower

20 g of dried butterfly bean flowers, Bdeliia outpost and cornflower flowers were prepared, washed with purified water, dried and mixed, and mixed with 600 g of a mixed solvent of ethanol and purified water in a ratio of 7:3 (w/w). The mixture was immersed for 3 days, and extracted by boiling at 100° C. for 8 hours in an extractor equipped with a cooling condenser. After inoculating the extract with Saccharomyces cerevisiae, it is fermented at 25°C for 3 days, sterilized at 95°C for 1 hour, filtered using Qualitive Filter Papers NO.2, and then in a filtrate state. Obtained.

Examples 2 to 7: Preparation of mixed fermented extracts of butterfly bean flowers, Bdeliia and cornflower flowers according to the mixing ratio

The weight ratios of the dried butterfly bean flower, budelia, and cornflower flower were adjusted as shown in the following table, and extracted in the same manner as in Example 1. The results are shown in Table 1 below.

division Mixing ratio Yield (%) Example 2 Butterfly Bean Flower, Bdelia and Cornflower = 1:1:2 84 Example 3 Butterfly Bean Flower, Bdelia and Cornflower = 1:2:1 82 Example 4 Butterfly Bean Flower, Bdelia and Cornflower = 1:2:2 88 Example 5 Butterfly Bean Flower, Bdelia and Cornflower = 2:1:1 88 Example 6 Butterfly Bean Flower, Bdelia and Cornflower = 2:1:2 90 Example 7 Butterfly Bean Flower, Bdelia and Cornflower = 2:2:1 88

Comparative Example 1: Preparation of a mixed extract of butterfly bean flower, Bdeliia and cornflower flower

Dried butterfly bean flowers, budelia and cornflower flowers were prepared, washed with purified water, dried, mixed, and mixed with 600 g of a mixed solvent of ethanol and purified water in a ratio of 7:3 (w/w). The mixture was immersed for 3 days and extracted by boiling at 100° C. for 8 hours in an extractor equipped with a cooling condenser. It was prepared in the same manner as in Example 6, except that it was not fermented with yeast.

Comparative Example 2: Preparation of mixed fermented extract of lactic acid bacteria of butterfly bean flower, cordelia and cornflower flower

In the method of Example 6, except for fermentation using Lactobacillus plantarum instead of Saccharomyces cerevisiae , it was carried out in the same manner, and Lactobacillus fermented butterfly bean flower, Brodelia and A mixed fermented extract of cornflower flowers was prepared.

Comparative Example 3: Preparation of a mixed extract of ultra-fermented butterfly bean flower, Bdeliia and cornflower flower

To the sample in which the dried butterfly bean flower, budelia, and cornflower flower were mixed in the same manner as in Example 6, 900 g of purified water containing 1% brown rice vinegar (Ottogi, total acidity 6-7%) was added and immersed in the solution. It was immersed for 1 day at room temperature. Thereafter, it was filtered using a filter paper (Qualitive Filter Papers NO.2), and then obtained as a filtrate to prepare a mixed ultra-fermented extract of butterfly bean flower, budelia and cornflower flower.

Test Example 1: Confirmation of increased expression of wrinkle-related factors

^{Fibroblasts (HDFa) were adjusted to 1×10 6} cells/well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), and then inoculated into 6 well plates and cultured for 24 hours. After incubation, the samples of Examples and Comparative Examples were treated and cultured for 48 hours. RNA was extracted from the cultured cells and the rate of increase was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The rate of increase in collagen expression was calculated by the following equation, and the results are shown in Table 2 below. Retinol palmitate was used as a positive control.

Collagen expression rate (%) = (Amount of collagen expression in sample/Amount of collagen expression in negative control)×100

Sample name Treatment concentration (w/v%) collagen expression rate (%) Example 6 0.5 194.1 Comparative Example 1 0.5 185.3 Comparative Example 2 0.5 187.2 Comparative Example 3 0.5 188.2 Retinol palmitate 0.5 185.3

As shown in Table 2 above, when the same concentration was treated, the fermented extract of the Example of the present invention exhibited a higher collagen expression rate compared to the positive control group and the comparative example.

Test Example 2: Confirmation of increase in wrinkle-related factor generation

^{Fibroblasts (HDFa) were adjusted to 1×10 6} cells/well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), and then inoculated into 6 well plates and cultured for 24 hours. After incubation, the samples of Examples and Comparative Examples were treated and cultured for 48 hours. The medium of the cultured cells was collected and the amount of procollagen was measured using a procollagen type I c-peptide (PIP) EIA kit, and the results are shown in Table 3 below. Retinol palmitate was used as a positive control.

Sample name Treatment concentration (w/v%) Procollagen production (%) Example 6 0.5 190.3 Comparative Example 1 0.5 180.5 Comparative Example 2 0.5 185.2 Comparative Example 3 0.5 183.7 Retinol palmitate 0.5 188.9

As shown in Table 3, when the same concentration was treated, the fermented extract of the Example of the present invention exhibited a higher amount of procollagen production compared to the positive control group and the comparative example.

Test Example 3: Confirmation of increased expression of skin elasticity-related factors

^{Fibroblasts (HDFa) were adjusted to 1×10 6} cells/well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), and then inoculated into 6 well plates and cultured for 24 hours. After incubation, the samples of Examples and Comparative Examples were treated and cultured for 48 hours. RNA was extracted from the cultured cells, and the rate of increase in expression of tropoelastin was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The increase rate of tropoelastin expression was calculated by the following formula, and the results are shown in Table 4 below. Retinol palmitate was used as a positive control.

Tropoelastin expression rate (%) = (Sample Tropoelastin expression amount/Negative control Tropoelastin expression amount)×100

Sample name Treatment concentration (w/v%) Tropoelastin expression rate (%) Example 6 0.5 193.3 Comparative Example 1 0.5 182.5 Comparative Example 2 0.5 184.7 Comparative Example 3 0.5 186.3 Retinol palmitate 0.5 188.9

As shown in Table 4, when treated with the same concentration, the fermented extract of the Example of the present invention exhibited a higher Tropoelastin expression rate compared to the positive control group and the comparative example.

Test Example 4: Confirmation of increased expression of skin moisturizing factors

^{Fibroblasts (HDFa) were adjusted to 1×10 6} cells/well using Medium 106 medium supplemented with Low Serum Growth Supplement (LSGS), and then inoculated into 6 well plates and cultured for 24 hours. After incubation, the samples of Examples and Comparative Examples were treated and cultured for 48 hours. RNA was extracted from the cultured cells, and the rate of increase in the expression of Hyaluronan synthase-2 (HAS-2) was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The increase rate of HAS-2 expression was calculated by the following formula, and the results are shown in Table 5 below. Hyaluronic acid was used as a positive control group.

HAS-2 expression rate (%) = (sample HAS-2 expression amount/negative control HAS-2 expression amount) × 100

Sample name Treatment concentration (w/v%) HAS-2 expression rate (%) Example 6 0.5 192.6 Comparative Example 1 0.5 184.3 Comparative Example 2 0.5 185.1 Comparative Example 3 0.5 188.2 Hyaluronic acid 0.01 187.3

As shown in Table 5, when the same concentration was treated, the fermented extract of the Example of the present invention exhibited a higher HAS-2 expression rate compared to the positive control group and the comparative example.

Test Example 5: Confirmation of skin inflammation alleviation effect

^{Keratinocytes (HEKa) were adjusted to 1×10 6} cells/well using Medium 154 medium supplemented with Human Keratinocyte Growth Supplement (HKGK), and then inoculated into a 96 well plate and cultured for 24 hours. The samples of Examples and Comparative Examples were treated and cultured for 18 hours, and then the cultured cells were removed from the medium, and 20 mJ/cm ² UVB was irradiated for 10 minutes to induce cell damage. RNA was extracted from the cultured cells, and the rate of decrease in the expression of Interleukin-6 (IL-6) was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The IL-6 expression reduction rate was calculated by the following formula, and the results are shown in Table 6 below. As a positive control group, the extract of machi-suki was used.

IL-6 expression rate (%) = (sample IL-6 expression amount / negative control IL-6 expression amount) × 100

Sample name Treatment concentration (w/v%) IL-6 expression rate (%) Example 6 0.5 32.8 Comparative Example 1 0.5 51.2 Comparative Example 2 0.5 47.3 Comparative Example 3 0.5 45.2 Portion string extract 0.5 40.1

As shown in Table 6, when the same concentration was treated, the fermented extracts of the examples of the present invention exhibited a lower IL-6 expression rate compared to the positive control group and the comparative example.

Test Example 6: Confirmation of skin regeneration effect

A wound healing assay was performed to confirm the skin cell regeneration effect. ^{Keratinocytes (HEKa) were adjusted to 1×10 6} cells/well using Medium 154 medium supplemented with Human Keratinocyte Growth Supplement (HKGK), and then inoculated into 6 well plates and cultured for 18 hours. After scratching a part of the cells, the samples of the Example and Comparative Example were treated and cultured for 8 hours. The cell regeneration rate was calculated by the following equation by measuring the wound gap, and the results are shown in Table 7 below. As a positive control, madecassocide was used.

Cell regeneration rate (%) = (sample cell regeneration rate/negative control cell regeneration rate) × 100

Sample name Treatment concentration (w/v%) Skin cell regeneration (%) Example 6 0.5 190.5 Comparative Example 1 0.5 180.5 Comparative Example 2 0.5 184.3 Comparative Example 3 0.5 182.6 Madecassoside 0.1 187.9

As shown in Table 7 above, when the same concentration was treated, the fermented extract of the Example of the present invention exhibited a higher skin regeneration rate compared to the positive control group and the comparative example.

Example 8: Preparation of lotion containing mixed fermentation extract

The lotion containing the mixed fermentation extract prepared in Example 6 was prepared according to a conventional method in the composition and content of Table 8 below.

(weight%) Example 8 Mixed fermentation extract (Example 6) 2 glycerin 8 Butylene Glycol 4 Caprylic/Capric Triglyceride 10 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol One Hexanediol 0.9 Ethylhexyl glycerin 0.05 incense 0.15 Purified water To 100

Claims (9)

A cosmetic composition for skin improvement containing a mixed fermented extract of butterfly bean flower, bedelaria and cornflower flower produced by fermenting a mixed extract of butterfly bean flower, budelia and cornflower flower with Saccharomyces cerevisiae. The cosmetic composition for improving skin according to claim 1, wherein the mixed fermented extract of butterfly bean flower, budelia and cornflower flower is contained in an amount of 0.01 to 90% by weight based on the total weight of the cosmetic composition. delete The cosmetic composition for improving skin according to claim 1, wherein the mixed extract is extracted by mixing butterfly bean flowers, bradelia and cornflower flowers in a weight ratio of 1 to 2: 1 to 2: 1 to 2, respectively. The cosmetic composition for improving skin according to claim 1, wherein the cosmetic composition is for improving skin wrinkles and elasticity. The cosmetic composition for improving skin according to claim 1, wherein the cosmetic composition is for moisturizing the skin. The cosmetic composition for improving skin according to claim 1, wherein the cosmetic composition is for relieving skin inflammation. The cosmetic composition for improving skin according to claim 1, wherein the cosmetic composition is for skin regeneration. (A) preparing a mixture by mixing a butterfly bean flower, a budelia and a cornflower flower in a weight ratio of 1 to 2: 1 to 2: 1 to 2, respectively;
(B) preparing a mixed extract by adding 5 to 10 times the weight of the solvent to the mixture and extracting at 80 to 100°C for 8 to 10 hours;
(C) fermenting the mixture extract with Saccharomyces cerevisiae for 3 to 5 days at 22 to 28° C. after inoculation; And
(D) After sterilization, filtering and concentrating a method for producing a fermented extract of a mixed fermented extract of a butterfly bean flower, a cordia flower, and a cornflower flower.