KR20110085371A - Cosmetic composition for improving the scalp state containing natural plant extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving the scalp state by containing a natural plant extract, more specifically, to inhibit the scalp inflammatory response by containing a natural plant complex components, including water extract, ginger extract and bamboo extract, It relates to a cosmetic composition excellent in the purification and soothing effect on the scalp by inhibiting the production and action of oxygen.

Description

Cosmetic composition for improving scalp condition containing natural plant extracts

The present invention relates to a cosmetic composition for improving the scalp state by containing a natural plant extract, more specifically, to inhibit the scalp inflammatory response by containing a natural plant complex components, including water extract, ginger extract and bamboo extract, It relates to a cosmetic composition excellent in the purification and soothing effect on the scalp by inhibiting the production and action of oxygen.

The scalp is composed of epidermis, dermis and subcutaneous tissue, with the outermost tissue being the epidermis. The epidermis functions to protect the sensitive tissues of the body from damage. However, various physical and chemical external stimuli such as external temperature, humidity, pollutants, stress, and lack of influence can cause the scalp to lose function, and can also affect hair loss.

Dandruff, on the other hand, is a stratum corneum-like mass formed by the dead or peeled cells of the scalp caused by the active metabolism of the human body with the secretion of the scalp sebaceous glands. Dandruff gradually has a clear border with normal skin, becomes red, and increases the scale of the scalp, which usually leads to seborrheic dermatitis with itching and inflammation.

Malassezia globosa, a dandruff bacterium of the scalp, is related to the occurrence of dandruff. In order to remove the dandruff, many chemicals such as zinc pyrithione, klimbazole, and imidazole are effective ingredients in many scalp care products. The material was mainly used.

However, the chemicals such as zinc pyrithione have excellent antibacterial activity against dandruff, but are less effective in relieving itching accompanying dandruff, and are not safe for skin side effects and chemicals. It can make consumers think negatively.

Therefore, there is a need for a composition containing a stable natural plant component while improving the condition of the scalp.

In this regard, the present applicants found that when using natural plant complex components including water lily extract, ginger extract, and bamboo extract, the anti-inflammatory and anti-oxidant effects were superior to those of using these components alone, so that the scalp purification and soothing effects were excellent. To complete.

Therefore, an object of the present invention is to provide a cosmetic composition excellent in improving the scalp condition.

In order to achieve the above object, the present invention provides a cosmetic composition for improving the scalp state containing a natural plant complex components, including water lily extract, ginger extract and bamboo extract.

The cosmetic composition according to the present invention effectively cleans the scalp by releasing toxins accumulated in the hair and scalp, and prevents scalp inflammation by inhibiting the growth and growth of dandruff, and also inhibits the production and action of free radicals. Excellent efficacy can provide an effect to strengthen the scalp.

Figure 1 shows the inhibitory effect of the scalp inflammatory response by the dandruff bacteria of the natural plant complex components the degree of expression of IL-8.
Figure 2 shows the inhibitory effect of the scalp inflammatory reaction by the fatty acid of the natural plant complex components the degree of expression of IL-8.
Figure 3 shows the results of measuring the antioxidant efficacy of the natural plant complex components by DPPH assay.
Figure 4 shows the antioxidant effect of the natural plant compound against ROS produced by fatty acids measured the antioxidant efficacy of the natural plant compound by the DCF-DA assay.
Figure 5 shows the antioxidant effect of the natural plant complexes for ROS produced by fatty acids and ultraviolet rays measured the antioxidant efficacy of the natural plant complexes by DCF-DA analysis.

The cosmetic composition of the present invention contains a natural plant complex component including water lily extract, ginger extract and bamboo extract as an active ingredient.

The water lily used in the present invention is a perennial aquatic plant, and many petioles grow in the thick and short undergrowth and fold the leaves on water. The leaf body is thick, egg-shaped, and the bottom part is deeply divided like an arrow. The front is green and glossy, and the back is purplish and thick. Water lily plants are beautiful because of their beautiful flowers, which are grown for ornamental purposes in ponds and greenhouses. Seeds and rhizomes also contain a lot of starch, which is edible all over the world. Root stems also contain nufariin, which is used as a gastrointestinal drug.

Ginger used in the present invention is also called a saeyang, a new sheep. It is native to Southeast Asia and is grown as a vegetable. Root stock grow sideways, fleshy, lumpy, yellow, with a spicy taste and aromatic odor. Root stems are dried and used as spices for breads, cookies, curry, sauces, and pickles. Peel and boil, add to syrup, and make ginger tea and ginger. In oriental medicine, dried root stem is used as a health medicine, which is effective for indigestion, vomiting and diarrhea, promotes blood circulation, and has anti-inflammatory and analgesic effects.

Bamboo used in the present invention is a generic term for perennial plants belonging to the monocotyledonous rice plant (Paceae, Poaceae) and the bamboo subfamily (Bambusoideae). Bamboo has a wide variety of uses, ranging from building materials, furniture, fishing rods, plant supports, to basketwork such as baskets, and is also planted for ornamental purposes. Young sprouts are cooked and eaten with herbs.

In the present invention, the extracts of water lilies, ginger and bamboo are added to ionized water after washing each dried raw material and extracted with hot water at 70 ~ 75 ℃. After cooling to room temperature, each extract is obtained by deodorization and filtration. The detailed manufacturing method is specified in the following reference example.

Water lily extract, ginger extract and bamboo extract included in the natural plant complexes used in the present invention are mixed in a weight ratio of 1 to 10: 1 to 10: 1 to 10, preferably synergistic due to the mixing of each plant extract It is recommended to mix at 1: 1: 1 to obtain the effect. In addition, the natural plant complex component contains a mixture of water lily extract, ginger extract and bamboo extract in an amount of 50.0 to 80.0% by weight based on the weight of the natural plant complex component. In addition, the natural plant complex component may further include a preservative widely used for bacteriostatic purposes in order to increase the preservation of the raw material, preferably butylene glycol and phenoxyethanol may be used.

In addition, the composition of the present invention contains the natural plant complex component in an amount of 0.01 to 10% by weight based on the total weight of the composition. This is because the effect is very weak at less than 0.01% by weight, there is a problem in the stability of the formulation above 10% by weight.

The composition of the present invention is not particularly limited in formulating, but may be formulated as a hair tonic, hair nourishing longevity, scalp treatment, hair treatments, hair shampoo, hair rinse, hair lotion, or scalp hair treatment. have.

In addition, in each formulation, other ingredients in addition to the above-mentioned essential ingredients may be appropriately selected and blended by those skilled in the art without difficulty according to the kind or purpose of use of other external preparations.

The composition according to the present invention effectively cleans the scalp by effectively discharging toxins accumulated in hair and scalp, and inhibits the growth and growth of dandruff bacteria to prevent scalp inflammatory reactions, and also inhibits the production and action of free radicals. This excellent soothing and strengthening of the scalp can provide the effect of strengthening the natural defenses.

Hereinafter, although an Example and a test example are given and demonstrated more concretely of this invention, this invention is not limited only to these examples.

Reference Example 1 Preparation of Water Lily Extract

After washing with water 1kg was added to 25L of ionized water and extracted with hot water for 3 hours at 70 ~ 75 ℃. After cooling to room temperature, the mixture was filtered using diatomaceous earth, treated with 0.5 kg of zeolite for deodorization, and filtered to obtain 21 L of an extract.

Reference Example 2 Preparation of Ginger Extract

2 kg of dried ginger was washed with water in 25 L of ionized water and extracted with hot water at 70-75 ° C. for 3 hours. After cooling to room temperature, the mixture was filtered using diatomaceous earth, treated with 0.5 kg of zeolite for deodorization, and filtered to obtain 21 L of an extract.

Reference Example 3 Preparation of Bamboo Extract

5 kg of dried bamboo was cut at intervals of 20 cm, washed with water, and poured into 25 L of ionized water and extracted with hot water at 70 to 75 ° C. for 3 hours. After cooling to room temperature, 20L of extract was obtained by filtration using diatomaceous earth.

Reference Example 4 Preparation of Natural Plant Complex Ingredients

Preservatives (1,3-butylene glycol 30% by weight after mixing 23.2% by weight of the bamboo extract, 23.2% by weight of the dry ginger extract, 23.1% by weight of the water lily extract, according to the ratio of the extract obtained in Reference Examples 1 to 3 , Phenoxyethanol 0.5% by weight) was mixed. This was pre-filtered using a 1.0 μm filter, and then packaged after micro-filtering using a 0.45 μm filter.

Test Example 1 Anti-inflammatory Effect by Natural Plant Compounds

Malassezia globosa decomposes fatty acids on the surface of the scalp using its Lipase. Among these decomposed fatty acids, unsaturated fatty acids penetrate into the scalp to cause pathological phenomena such as inflammatory reactions, and saturated fatty acids are used as energy sources for growth and growth of dandruff. In addition, fatty acids produced by dandruff and dandruff in the scalp may increase the expression of IL-8 in the scalp keratinocytes and induce scalp inflammation. Therefore, in this test example, the natural plant compound of the present invention was to determine the effect of inhibiting the skin inflammatory response caused by dandruff and fatty acids.

Malassezia globosa and typical fatty acids (linoleic acid) of the skin were treated to human keratinocytes and inflammatory responses were analyzed and anti-inflammatory effects were confirmed through analysis of inflammatory mediators. The specific experimental method is as follows.

1) Cell Culture

HaCaT cells, human immortalized keratinocytes, were placed in DMEM (Dulbecco Modified Eagle's Medium) containing 10% FBS (Fetal Bovine Serum) and 100unit / ml penicillin-streptomycin and cultured at 37 ° C. and 5% CO ₂ .

2) Dandruff culture and dandruff sample preparation

Dandruff (Malassezia globosa) was purchased from ATCC (ATCC strain # 96807), inoculated in Leeming & Notman broth (broth) and used in the experiment after proliferation for 3 days at 35 ℃. The grown dandruff was centrifuged and separated into dandruff and culture medium. The culture medium was stored frozen without further purification, and then dissolved and used when necessary. Dandruff was washed three times with PBS (Phosphate Buffered Saline), and then refrigerated at 4 ° C and used for the test.

3) IL-8 analysis

HaCaT cells were seeded in 24-well plates at 1 × 10 ⁵ concentrations. The day after seeding, the FBS concentration was lowered to 2%, exchanged with IL-8 assay medium without phenol red, and treated with dandruff and fatty acids. After 24 hours of material treatment, the culture medium of the cells was collected, and the remaining cells were washed twice with PBS and then evaluated for cytotoxicity using the WST-1 (Roche) reagent. The collected medium was centrifuged to remove the residue and the IL-8 concentration in the medium was measured using an IL-8 ELISA set (BD Bioscience). WST-1 and IL-8 ELISA followed the protocol provided by the manufacturer.

4) Statistical Processing

The concentration of IL-8 measured in the medium was divided by the value of WST-1 to correct for the difference in values due to cytotoxicity. The significance of each group was evaluated by using the Minitab statistical program.

The measurement results are shown in FIGS. 1 and 2.

1 and 2, when the ginger extract is treated alone, it can be seen that the inhibitory effect of the scalp inflammatory reaction by dandruff and fatty acids. However, natural plant complexes, including water lily extract, ginger extract and bamboo extract, can suppress the increase of IL-8 by dandruff and fatty acids in a concentration-dependent manner, especially when the natural plant complex is treated at a concentration of 10 ppm It can be seen that the scalp inflammatory response by about 63%, it can suppress the scalp inflammation response by fatty acids about 65%.

Through this, dandruff bacteria can induce scalp inflammation by increasing the expression of IL-8 mediators in scalp keratinocytes, but the natural plant complex consisting of water extract, ginger extract and bamboo extract is the scalp It can be seen that it can provide an anti-inflammatory effect by inhibiting the inflammatory response.

Test Example 2 Antioxidant Effect by Natural Plant Compounds

ROS (reactive oxygen stress) refers to free radicals containing oxygen atoms. When a cell is continuously affected by various external stimuli such as by-products generated during metabolism or ultraviolet rays, As a result, the amount of ROS produced in cells increases rapidly. This increased ROS affects the cell's DNA, RNA, and proteins, causing various phenomena that damage the cell, such as cell necrosis or inflammatory responses. Therefore, in this test example, the antioxidant effect to prevent the production and action of ROS produced by fatty acids to the natural plant complex of the present invention was measured, DPPH analysis and DCF-DA analysis was used.

1. DPPH Method

DPPH is a stable free radical with a reddish red color, and when the number of free radicals decreases due to antioxidant potency, it gradually turns yellow, thereby determining the antioxidant potency of the substance against ROS generated in the cell.

1) Preparation of DPPH (2,2-Diphenyl-1-picrylhydrazyl)

Before starting the experiment, it was dissolved in ethanol to 100 μM.

2) preparation of treated materials

The material to react with the DPPH solution was dissolved in ethanol at the final treatment concentration x20.

3) Material treatment

Substances to be treated were placed in 96-well plates at 10 μl per concentration. At this time, the number of treatments having the same concentration was repeated three times for each substance treatment concentration section. 10 μl of ethanol was added to the control group.

4) DPPH Treatment and Reaction

100 μM DPPH was added in 190 μl each using a multichannel pipette. Then stored for 30 minutes in a 37 ℃ incubator.

5) absorbance measurement and statistical processing

Absorbance at 517 nm was measured using Spectra Max 190. The rate of change of the material treatment group was calculated based on the control group. The significance of each group was assessed using the Minitab statistical program.

The results are shown in FIG.

In Figure 3, the ginger extract 50μM single treatment test group, but significant free radical scavenging ability is observed, it can be seen that the concentration is higher than the natural plant compound used, and the efficacy is much lower. On the other hand, natural plant complexes consisting of water lily extract, ginger extract and bamboo extract reduced the amount of free radicals to about 60% of the control group when treated at a concentration of 10 μM, and also when tested at 95% confidence level, Antioxidant activity is observed at both 10 and 5 μM concentrations, and it can be seen that the natural plant composites of the present invention show a concentration-dependent antioxidant activity.

2. DCF-DA Method

This method is a method capable of measuring the amount of intracellular ROS produced. Specifically, when DCFH-DA enters the cell, it is present in the DCFH state, where hydrogen peroxide (H ₂ O ₂ ) generated in the cell is converted into a highly fluorescent DCF. At this time, if the green light becomes strong or the fluorescence value is high through fluorescence measurement and observation of the DCF, the ROS generation may be determined to be high.

(1) Antioxidative Effect of Natural Plant Compounds on ROS Produced by Fatty Acids

1) Cell Culture

Dulbecco Modified Eagle's Medium containing 10% FBS (Fetal Bovine Serum) and 100 unit / ml penicillin-streptomycin was laid down on an Ibidi 8-well plate with 1.0 × 10 ⁵ / well HaCaT cells, human immortalized keratinocytes. ) Was incubated for 24 hours at 37 ℃, 5% CO ₂ .

2) material processing

The substance treatment group was placed in a serum-free medium to be evaluated and pretreated for 24 hours. At this time, the experiment was carried out with a concentration of the substance does not appear cytotoxic. The other groups also changed to serum-free medium.

3) DCF da treatment

The medium was removed and washed once with HBSS. DCF da 50 μM was treated and reacted in an incubator for 20 minutes. After the reaction, the mixture was washed twice with HBSS.

4) Stimulation source treatment and confocal microscopy

After treatment with 100 μM of fatty acid (OA: oleic acid), 1 hour later was observed at 488nm wavelength with a confocal microscope. The substance treatment group treated the evaluation substance together in the fatty acid treatment.

The results are shown in FIG.

Referring to Figure 4, the treatment with fatty acids (OA) increases the amount of intracellular ROS production, it can be seen that the increase in ROS production by fatty acids is suppressed when the natural plant complex component according to the present invention.

(2) Antioxidant Effect of Natural Plant Compounds on ROS Produced by Fatty Acids and Ultraviolet Rays

1) Cell Culture

Dulbecco Modified Eagle's Medium containing 10% FBS (Fetal Bovine Serum) and 100 unit / ml penicillin-streptomycin was laid down on an Ibidi 8-well plate with 1.0 × 10 ⁵ / well HaCaT cells, human immortalized keratinocytes. ) Was incubated for 24 hours at 37 ℃, 5% CO2.

2) material processing

The substance treatment group was placed in a serum-free medium to be evaluated and pretreated for 24 hours. At this time, the experiment was carried out with a concentration of the substance does not appear cytotoxic. The other groups also changed to serum-free medium.

3) DCF da treatment

The medium was removed and washed once with HBSS. DCF da 50 μM was treated and reacted in an incubator for 20 minutes. After the reaction, the mixture was washed twice with HBSS.

4) Stimulation source treatment and confocal microscopy

The control group not treated with UVB was covered with an absorbent film and treated with UVB 30mJ. After the UVB treatment, the fatty acid (OA: oleic acid) 100μM treatment, after 1 hour was observed with a confocal microscope at 488nm wavelength. The substance treatment group treated the evaluation substance together in the fatty acid treatment.

The results are shown in FIG.

Referring to Figure 5, the treatment with fatty acids (OA) and ultraviolet light increases the amount of ROS production in the cell, the treatment of the natural plant compound according to the present invention can be confirmed that the increase in the production of ROS by fatty acids and ultraviolet light is suppressed. have.

Test Example 3 Efficacy and Quality Test

After the shampoos of Examples 1 to 2 and Comparative Example 1 of Table 1 were used daily for two weeks for a panel consisting of 30 male and 20 adult males and females, 7 points were included in the following questionnaire. The quality of use was assessed by answering the scale (higher scores are better). The results are shown in Table 2 below.

ingredient Comparative Example 1
(Unit: weight%) Example 1
(Unit: weight%) Example 2
(Unit: weight%) Purified water To 100 To 100 To 100 Ammonium lauryl sulfate solution (active ingredient 25%) 28 28 28 Ammonium Laureth Sulfate Solution (Active Content 25%) 20 20 20 Phenoxyethanol 0.2 0.2 0.2 Natural Plant Compounds (Reference Example 4) - 0.1 One Guarhydroxypropyltrimonium chloride 0.4 0.4 0.4 Cetyl alcohol One One One Dimethicone One One One Citrix Acid 0.1 0.1 0.1 Trihydroxystearin 0.3 0.3 0.3 Spices 0.7 0.7 0.7

Survey item Comparative Example 1 Example 1 Example 2 1. Air bubbles are good and air bubbles feel good 5.4 5.4 5.5 2. Feels good when rinsing and fingers pass well 5.6 5.8 5.7 3. Easy to comb and soft when dried 5.6 5.7 5.6 4. Itching of the scalp after drying is improved. 3.8 4.5 6.2 5. After 2 weeks, the scalp was clean.
(No rash or erythema on the scalp) 4.0 4.9 6.5

Looking at Table 2, when using Examples 1 to 2 according to the present invention it can be confirmed that the itching of the scalp is much improved and the state of the scalp is clean than when using Comparative Example 1.

Claims (7)

Cosmetic composition for improving the scalp state containing a natural plant complex components, including water lily extract, ginger extract and bamboo extract. The cosmetic composition according to claim 1, wherein the natural plant complex component comprises a water lily extract, a ginger extract, and a bamboo extract in a ratio of 1 to 10: 1 to 10: 1 to 10. The cosmetic composition according to claim 1, wherein the mixed amount of the water lily extract, the ginger extract and the bamboo extract is 50.0 to 80.0% by weight based on the weight of the natural plant complex component. The cosmetic composition according to claim 1, wherein the natural plant complex component is contained in an amount of 0.01 to 10% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the natural plant composite component further comprises butylene glycol and phenoxyethanol. The cosmetic composition according to claim 1, wherein the composition inhibits inflammation of the scalp. According to claim 1, wherein the composition is a cosmetic composition, characterized in that for antioxidant.

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