KR20150002048A - A composition for preventing wrinkle of skin and anti aging of skin - Google Patents

Abstract

The present invention relates to a composition for anti-wrinkle and anti-aging. The composition of the present invention comprising extracts of Polygonatum sibiricum roots, Dioscorea Rhizoma roots, Cistanche deserticola stems, Panax notoginseng roots, and saffron increases biosynthesis of collagen in skin cells, thereby increasing expression of collagen type 1 increasing collagen which is a main component of skin. Therefore, the composition of the present invention can be useful as the composition for anti-wrinkle and anti-aging such as a cosmetic composition, a pharmaceutical composition, a health supplement composition, and an external use skin formulation.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing skin wrinkles and skin aging,

The present invention relates to a composition for improving skin wrinkles and preventing skin aging. More specifically, the present invention relates to a composition for improving skin wrinkles and preventing aging of skin, which comprises as an active ingredient, an extract of Hwangjuk rhizome, rhizomes rhizome, ≪ / RTI >

The skin of the human skin consists largely of the epidermis, the dermis, and the subcutaneous fat layer. The basic role of the skin is to prevent water from evaporating from our body and to prevent harmful substances from invading from the outside. These skin are always exposed to external stimuli, so they have various defenses. In addition, it can be said that skin is healthy and clean is related to human beauty. In recent years, not only women but also men have been interested in various skin troubles such as skin aging, and a lot of related products are being developed.

As people get older, skin aging occurs. A typical symptom is wrinkle. Wrinkles are a phenomenon of age, and the most common cause of wrinkles is the degradation of collagen that forms the matrix in the dermis of the skin. Skin collagen is decreased in aging process, but collagen is easily decomposed due to increased activity of collagenase (Matrix Metallo Proteinase, MMP-1) due to external stimuli such as ultraviolet rays, resulting in increased production of wrinkles There are many clinical reports. Therefore, in recent years, many products have been developed which inhibit the inhibition of the production of MMP-1 and the activity thereof, thereby suppressing the decomposition of collagen, which is basically produced.

MMP is a calcium and zinc-dependent endopeptidase secreted from cells such as polymorphonuclear neutrophils, macrophages, fibroblasts, etc., and acts at neutral pH. Use the substrate of the cell. MMP-1, MMP-2, and MMP-9 are the most active enzymes in the skin, and the most fundamental enzyme involved in collagen degradation is MMP-1. In general, there are retinoids (RE36068), TGF-beta (transforming growth factor), betulinic acid (JP8-208424), and the like, which inhibit the production of MMP- β (transforming growth factor), and various other natural products are known to inhibit MMP-1 biosynthesis. Therefore, a method for inhibiting wrinkle formation can be used, which promotes collagen synthesis or inhibits the production and activity of collagenase, and there is a desperate need to develop a cosmetic for more effective, easy and safe wrinkle improvement It is true.

In recent years, consumer's desire for anti-aging and anti-wrinkle products has increased as aesthetic desires for anti-aging of consumers have increased. In addition, as consumer interest in these functional products increases, so does demand.

Accordingly, the present inventors have made a study to develop a natural plant extract for improving skin wrinkles. As a result, the present inventors have found that a composition comprising Hwangjeong rhizome extract, Rhizoma root extract, Sarcodon rhizome extract, Trichothecaster rhizome extract and saffron extract increases collagen I type (collagen type I), and thus it can be effectively used as an effective ingredient of a composition for improving skin wrinkles and preventing skin aging. The present invention has been completed based on this finding.

The present invention provides a composition for preventing skin wrinkles and skin aging which contains natural extracts.

In order to accomplish the above object, the present invention provides a cosmetic composition for improving skin wrinkles and skin aging, which contains, as an active ingredient, an extract of Hwangjeong rhizome extract, Rhizoma root rhizome extract, Rhizoma stem extract, Tricholoma root extract and saffron extract .

In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles and preventing skin aging, which comprises as an active ingredient, Hwangjeong Rhizome extract, Rhizoma Rhizome Extract, Sarcoma Stem Extract, Tricholoma Rhizoma Extract and Saffron Extract.

The present invention also provides a composition for a health food for improving skin wrinkles and preventing aging of skin, which comprises as an active ingredient an extract from Hwangjeong Rhizoma extract, Rhizoma root extract, a stem extract for breeding, a Rhizoma root extract, and a saffron extract.

In addition, the present invention provides a skin external preparation for skin wrinkle improvement and skin aging prevention, which contains, as an active ingredient, Hwangjeong Rhizome extract, Rhizopus root extract, Sarcoma stem extract, Trichomonax root extract and saffron extract.

The composition of the present invention comprising collagen type I extract, collagen type I extract (collagen type I) which increases collagen biosynthesis in skin cells and increases collagen, which is a main component of the skin, 1), it is expected that it can be very usefully used in compositions for improving skin wrinkles and skin aging, for example, cosmetic compositions, pharmaceutical compositions, compositions for health foods, and external preparations for skin.

BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments of the invention and, together with the description of the invention, It should not be construed as limited.
FIG. 1 is a graph showing the effect of increasing expression of collagen type I on the extracts of Rhizopus rhizome, Rhizoma root extract, Rhizoma stem extract, Rhizopus root extract and saffron extract.

The present invention provides a cosmetic composition for improving skin wrinkles and preventing skin aging, which comprises as an active ingredient an extract from Hwangjeong Rhizoma extract, Rhizoma root extract, a stem extract for breeding, a Rhizoma root extract, and a saffron extract.

Saemyong rhizome extract, Rhizoma root extract, Sarcoma stem extract, Trichothecia rhizoma extract and Saffron extract are mixed at a weight ratio of 5: 1: 2.5: 0.5: 2.5.

The extracts of Rhizopus rhizome root extract, rhizome root extract, seedling stem extract, Rhizopus root extract and saffron extract may be prepared by extracting each of the raw materials with an extraction solvent, filtering and concentrating the same at reduced pressure, and then drying and pulverizing.

The hymen rootstock, the rootstock rootstock, the stem for breeding, the rootstock rootstock, and the saffron can be used without limitation, such as cultivated or commercially available ones.

Polydonati rhizoma used in the present invention is a perennial herbaceous plant of pizza plant gland> camphor tree plant> lily tree> lily and is a perennial herb which is distributed in northern part of Korea, Russia, China and Mongolia. It grows in the slope of the wilderness, the bark of the mountain, and the shrubs. It is cultivated in Duksan of Jecheon City in Chungbuk. The taste is sweet, the weakness is mediocre. It enters the spleen, lung (lung), and nerve (肾 经). It is known to be efficacious for leukopenia, myopia, limbic rhinitis, pulmonary tuberculosis and chronic bronchitis, and hyperlipemia.

As used in the present invention, the term " glutinosaccharide " Its mood is sweet and flat and non-toxic, mainly in the kidneys of the spleen and gastrointestinal tract. The efficacy is bibyeong (complement 脾 Stomach) and the lungs and kidneys to benefit the benefits of the 肝 肺 (益 肺 肾) action. It is used for symptoms such as malaise, helplessness, anorexia and diarrhea due to non-functional weakness, because it shows bigger 脾气 and shows favorable response to nasal (脾 阴). do. It is effective in the absence of the weakness and seawater, asthma and sputum due to lack of lung (肺气) and pulmonary sound (肺 阴), or in the case of few and sticky symptoms. It is also used to treat symptoms of weakness and weakness in the back and knee due to groaning (肾阴虛), symptoms of sperm flowing spontaneously, frequent premature ejaculation and urination, or peeing irrespective of one's own will. It contains mucus, saponin, starch, vitamin C, etc. Its pharmacological report shows that it acts on the digestive system and shows a remarkable effect on the intestinal activity of the intestinal tract, lowering of blood sugar and anti-aging action It has been reported to have an effect of extending the life span.

There are two kinds of tinnitus for use in the present invention, such as a sardine, a sardine, a sardine, a sardine, a bongbu, a sardine, and a hemorrhoidal tongue, such as meat roots, pine needle roots, and black rods. Especially, the stem is used as medicine, and the taste is sweet and weave. It treats 5 勞 (Oro) and 7 七 (상)), shows 脾 胃 (胃), heals 寒 痛 (肝 胴) It strengthens the 阴气 (声气), energizing 气气 (period), the descendants of a lot and the wife (Jinga) is cured. It is said that it has the effect of lightening the body when taken long.

The trichotomy used in the present invention is Panax notoginseng FH Chen, which is called Panax notoginseng FH Chen, which is called Panzanil, Panzhil, Goldfang, Goldsilver, It is a perennial herb that belongs to Araliaceae. Samchil is a very important medicine for hemostasis applied to all hemorrhages inside and outside the body. Especially, it smoothes the flow of blood and eliminates the eosinophilia. It is used for various bleeding symptoms, abdominal pain caused by eosinophilia, boils, pain, and a lot of blood in the postpartum. It is also known to treat swelling, sore symptoms, swelling, and swelling of bruises and fractures with the efficacy of lowering swelling and stopping pain.

The saffron used in the present invention is a spice made by drying the style of a saffron crocus (Crocus sativus) flower, which is a herbaceous plant group> a cotyledonous plant group> asparagus tree> belonging to an iris family. There are three styles and this part is dried and used as seasoning or dye in cooking. It also has medical uses.

Not only has saffron been used as part of folk remedies for a long time, but modern medicine has also been shown to have anticancer and antioxidant effects. Saffron has also been used as a textile dye in many countries, including China and India, and also as a spice.

The extraction solvent is preferably, but not limited to, water, alcohol or a mixture thereof. As the alcohol, C1 to C4 lower alcohols are preferably used. As the lower alcohol, ethanol or methanol is preferably used, but not limited thereto. As the extraction method, it is preferable to use shaking extraction or reflux extraction, but it is not limited thereto.

The extraction solvent is preferably added by 2 to 10 times the amount of the natural raw material, but not limited thereto. The extraction temperature is preferably from room temperature to 100 ° C, but is not limited thereto. The extraction time is preferably 2 to 5 days, but is not limited thereto. The number of times of extraction is preferably 1 to 3 times, but is not limited thereto.

In the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator at a reduced pressure, but not always limited thereto. The drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but is not limited thereto.

In a specific example of the present invention, 70% ethanol was added to a natural raw material in an amount of 10 times that of the sample at room temperature and filtered under reduced pressure to obtain each extract.

Also, in another specific embodiment of the present invention, collagen type 1 (collagen type 1) produced by the above-mentioned cultured horsetail extract, horseradish root extract, seedling stem extract, horseradish root extract and saffron extract in human fibroblast, The compositions containing the Hwangjeong Rhizome extract, Rhizoma Rhizoma extract, Sarcoma stem extract, Trichomonic Rhizome extract and Saffron extract according to the present invention, preferably in a weight ratio of 5: 1: 2.5: 0.5: 2.5, Lt; RTI ID = 0.0 > I < / RTI > type.

Therefore, the composition comprising the extract of Rhizopus rhizome extract, Rhododendron rhizome extract, Sarcoma stem extract, Trichomere rhizome extract and saffron extract has excellent collagen type 1 expression enhancement effect and is excellent in anti-wrinkle effect, The present invention can be effectively used as a cosmetic composition.

The cosmetic composition may be in the form of a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome) A cream, a skin, a lotion, a powder, an ointment, a spray, or a cone stick. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

The cosmetic composition of the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening agents and gelling agents, softening agents, antioxidants, suspensions, A stabilizer, a foaming agent, a perfume, a surfactant, water, an ionic or nonionic emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, a wetting agent, Pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics.

In the cosmetic composition containing the rhizome root extract of the present invention, rhizome root extract, sarcoma stem extract, Rhizoma root extract and saffron extract, the extract of the present invention is added to the cosmetic composition usually contained in an amount of 1 to 15% by weight, May be added in an amount of 2 to 10% by weight.

In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles containing as an active ingredient an extract from Hwangjeong Rhizome, a Rhizoma root extract, a stem extract for breeding, a Rhizoma root extract, and a saffron extract.

The present invention relates to a method for improving wrinkles and preventing aging of skin, which has excellent collagen I type expression because it has excellent collagen I type expression enhancing effect, Can be usefully used as an emulsion composition.

The pharmaceutical composition of the present invention may further contain commonly used excipients, disintegrants, sweeteners, lubricants, flavors and the like, and may be formulated into tablets, capsules, powders, granules, suspensions, Syrups, and other liquid preparations.

Specifically, the pharmaceutical compositions of the present invention can be formulated for oral administration, for example, as tablets, troches, lozenges, aqueous or aqueous suspensions, prepared powders or granules, emulsions, hard or soft capsules, It is formulated into elixirs. Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, celluloses or gelatin, excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, and disintegrants such as stearic acid Magnesium stearate, calcium stearate, sodium stearyl fumarate, or polyethylene glycol wax. In the case of a capsule formulation, in addition to the above-mentioned substances, a liquid carrier such as fatty oil is contained.

In addition, the pharmaceutical composition of the present invention can be administered orally or parenterally, and it is preferable to select subcutaneous injection, intravenous injection, intramuscular injection, or intra-thoracic injection injection method for parenteral administration. In order to formulate the formulation for parenteral administration, the suspension of the present invention is prepared by mixing the Rhizopus rhizome extract, Rhododendron rhizome extract, Sarcoma stem extract, Trichomonic Rhizome extract and saffron extract with water together with a stabilizer or a buffer in water to prepare a suspension, Formulated into a dosage form.

The dosage of the active ingredient according to the present invention is appropriately selected depending on the degree of absorption, inactivation rate and excretion rate of the active ingredient in the body, the age, sex and condition of the patient, and severity of the disease to be treated. The extract of the present invention may be administered to an adult in an amount of 0.0001-500 mg per 1 kg of body weight per day, preferably in an amount of 0.001-100 mg per one kg of body weight.

The present invention also provides a composition for a health food for improving skin wrinkles and preventing aging of skin, which comprises as an active ingredient an extract from Hwangjeong Rhizoma extract, Rhizoma root extract, a stem extract for breeding, a Rhizoma root extract, and a saffron extract.

The Rhizophora Rhizome Extract, Rhizoma Rhizome Extract, Saffron Rhizome Extract, Rhizophora Rhizoma Extract, and Saffron Extract have an excellent effect of enhancing collagen I type expression, and thus can be effectively used as a health food for improving skin wrinkles and preventing skin aging .

The health food of the present invention can be used as it is, or can be used together with other food or food ingredients, and can be suitably used according to a conventional method.

There is no particular limitation on the kind of the food. Examples of the foods which can be added with the Hwangjeong Root Extract, Root Root Extract, Root Root Extract, Root Root Extract, Root Root Root Extract and Saffron Root Extract include meat, sausage, bread, chocolate, candy, snack, confectionery, , Dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the present invention also relates to a method for the treatment and / or prophylaxis of various diseases such as rhizome root rhizome extract, rhizome root rhizome extract, seedling stem extract, Trichomonium rhizome extract and saffron extract according to various nutrients, vitamins, electrolytes, flavor, colorant, pectic acid and salts thereof, Protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

In addition, the present invention provides a skin external preparation for skin wrinkle improvement and skin aging prevention, which contains, as an active ingredient, Hwangjeong Rhizome extract, Rhizopus root extract, Sarcoma stem extract, Trichomonax root extract and saffron extract.

The Rhizophora Rhizome Extract, Rhododendron Rhizome Extract, Rhododendron Rhizoma Extract, Rhizophora Rhizoma Extract, and Saffron Rhizome Extract and Saffron Extract have an excellent effect of enhancing collagen I type expression, and thus can be effectively used as a skin external agent for improving skin wrinkles and preventing skin aging .

When the extract of the present invention is used as an external preparation for skin, it may further contain a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, A preservative, a stabilizer, a foaming agent, a perfume, a surfactant, water, an ionic or nonionic emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, , Pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient conventionally used in external preparations for skin. In addition, the components can be introduced in amounts commonly used in the dermatology field.

The dose of the composition comprising the Hwangjeong rhizome extract, the Rhizoma root extract, the stem extract, the Rhizoma root extract and the saffron extract to the external preparation for skin is 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg, But is not limited to. The dose may vary depending on the weight, age, sex, health condition, administration period, elimination rate, severity of disease, etc. of the particular patient.

In addition, the present invention provides a color cosmetic composition containing an extract from Hwangjeong Rhizoma extract, Rhizoma Rhizoma extract, a stem extract for breeding, a Rhizoma root extract and a saffron extract.

The color cosmetic composition of the present invention can be prepared in any formulations conventionally prepared and is preferably used in any one form selected from the group consisting of lipstick, eyeliner, makeup foundation, mascara, ball touch, and eye shadow .

The composition comprising the Hwangjeong rhizome extract, Rhizoma rhizome extract, Sarcoma stem extract, Trichomere rhizome extract, and saffron extract in the color cosmetic composition using the Rhizopus rhizome extract, Rhododendron rhizome extract, Rhododendron rhizome extract, Sarcoma stem extract, May be contained in an amount of 0.0001 to 50% by weight, more preferably 0.0001 to 30% by weight, based on the whole cosmetic composition, but is not limited thereto.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Example 1 Preparation of Rhizoma Root Extract

After incubation at room temperature for 24 hours, 70% ethanol was added to the seedlings at room temperature for 24 hours. Then, the mixture was vacuum-filtered (Whatman filter paper # 2) And used.

Example 2: Preparation of Rhizome Rhizoma Extract

After incubation at room temperature for 24 hours, 70% ethanol was added to the sample and the mixture was filtered at reduced pressure (Whatman filter paper # 2), dried and stored at -20 ℃. And used.

Example 3: Preparation of stem extract for breeding

The seeds for breeding were obtained and leached at 70% ethanol for 10 hours at room temperature for 24 hours and then filtered under reduced pressure (Whatman filter paper # 2), dried and stored at -20 ℃. And used.

<Example 4> Preparation of extract of Triloba rhizome

The extract was filtered through vacuum filtration (Whatman filter paper # 2), dried and pulverized, stored at -20 ° C, and immediately after the experiment, And used.

<Example 5> Preparation of Trichotillomania root extract

Saffron was obtained and leached at 70% ethanol for 10 hours at room temperature for 24 hours. Then, it was filtered at reduced pressure (Whatman filter paper # 2), dried and stored at -20 ℃. Respectively.

Test Example 1 Confirmation of Increase of Collagen Type 1 Expression in Human Fibroblast Cells

Human fibroblast cells (ATCC-2076) were cultured in human fibroblasts in order to examine the effect of collagen I on the expression of collagen I in skin cells of rhizome root extract, rhizome root extract, seedling stem extract, , USA) was inoculated with IMCD medium (Iscove's Modified Dulbecco's Medium, GIBCO) supplemented with 10% FBS (GIBCO) in a 60 mm culture dish (NUNC, USA) at a density of 1 × 10 6 cells for 24 hours . After incubation for 24 hours, the cells are washed with HBSS and treated with IMDM medium without FBS. The samples are treated for 24 hours at 37 ° C in a 5% CO2 incubator (REVCO, USA).

After culturing, the cells were collected in 1 ml of TRIzol (Invitrogen, USA), transferred to a 1.5 ml tube, and 200 μl of chloroform (Sigma, USA) was added thereto and vortexed for 5 minutes at room temperature. After centrifugation at 15,000 rpm for 30 minutes, the supernatant is transferred to a new tube, mixed with an equal volume of 2-propanol, allowed to stand at room temperature for 5 minutes, and then centrifuged at 12,000 rpm for 20 minutes at 4 ° C. After centrifugation, discard 2-propanol, add 1 ml of 75% ethanol, centrifuge at 10,000 rpm for 10 minutes at 4 ° C, discard 75% ethanol, and remove remaining ethanol from the RNA pellet at room temperature. Add 60 μl of DEPC-treated purified water to the pellet and dissolve it.

Real-time PCR was performed using the Real-time PCR Kit (Power SYBR Green PCR Master Mix, Applied Biosystems, CA, USA) and was performed using the kit method. Applied Biosystems 7500 FAST Real-Time PCR System , CA, USA), and amplified products were quantitatively analyzed. Collgen Type I and Actin primers used in PCR were synthesized in Bioneer (Korea) and the sequence is shown in Table 1 below.

At this time, the extracts of Hwangjeong rhizome were used at a concentration of 100 ㎍ / ㎖, 250 ㎍ / ㎖ and 500 ㎍ / ㎖, Saffron extracts were used at concentrations of 50 / / ㎖, 100 ㎍ / ㎖ and 250 ㎍ / ㎖, 50 ㎍ / ㎖, 100 ㎍ / ㎖ and 250 ㎍ / ㎖, respectively. Saffron extract Was used to measure the expression level of collagen type I in fibroblasts at concentrations of 100 ㎍ / ㎖, 250 ㎍ / ㎖ and 500 ㎍ / ㎖ by Realtime PCR.

As a comparative group, 100 μg / ml of vitamin C (Sigma, A4403) was used, and purified water was used as a control group.

The results are shown in Fig. As shown in FIG. 1, the expression of collagen I type in the experimental group for 100% of the comparative group was found to increase at all concentrations of all the medicines except for the concentration of 250 μg / ml of the root muscle.

Example 6: Preparation of composition for improving skin wrinkles and preventing skin aging

A composition for improving skin wrinkles and preventing aging of the skin was prepared by mixing the extracts of Hwangjeong Rhizoma extract, Rhododendron rhizome extract, Rhizoma stem extract, Rhizopus rhizome extract and saffron extract at a weight ratio of 5: 1: 2.5: 0.5: 2.5 .

The expression level of collagen I type was measured in the same manner as in Test Example 1, and the expression level of the collagen I type was increased by 211% as compared with that of the control group 100.

As can be seen from the above results, the composition comprising the Hwangjeong Rhizome extract, Rhizoma Rhizoma extract, Sarcoma stem extract, Trichomere rhizome extract and Saffron extract according to the present invention has superior effects than expected in view of the effects obtained from the respective extracts It is possible to obtain the skin wrinkle improvement and the skin aging prevention effect.

&Lt; Formulation Example 1 > Preparation of a cosmetic comprising an extract of Rhodophyta rhizome, Rhododendron rhizome extract, Rhododendron stem extract, Rhizopus rhizome extract and saffron extract as active ingredients

<1-1> Manufacture of flexible lotion

The flexible lotion containing the Hwangjeong rhizome extract, the Rhizoma root extract, the stem extract, the Rhizoma root extract and the saffron extract as an active ingredient of Example 6 was prepared as shown in Table 2 below.

<1-2> Preparation of nutritional cream

The nutritional cream containing the Hwangjeong rhizome extract, the Rhizoma root extract, the stem extract, the Rhizopus root extract, and the saffron extract as an active ingredient of Example 6 was prepared as shown in Table 3 below.

&Lt; Formulation Example 2 > Preparation of medicaments containing Hwangjeong rhizome extract, Rhizoma rhizome extract, Sarcoma stem extract, Trichomere rhizome extract and saffron extract as active ingredients

&Lt; 2-1 > Preparation of syrup preparation

The syrup preparation containing the Hwangjeong rhizome extract, the Rhizoma root extract, the stem extract, the Rhizoma root extract and the saffron extract as an active ingredient of Example 6 was prepared as shown in Table 4 below.

<2-2> Preparation of tablets

The tablets containing the Hwangjeong Root Extract, the Rhizoma Rhizome Extract, the Stem Extract, the Trichomonic Rhizome Extract, and the Saffron Extract of Example 6 as an active ingredient were prepared as shown in Table 5 below.

A mixture of 250 parts by weight of a mixture of the rhizome root ending extract of Example 6, Rhododendron rhizome extract, Sarcoma stem extract, Rhizoma root extract and saffron extract, 175.9 parts by weight of lactose, 180 parts by weight of potato starch and 32 parts by weight of colloidal silicic acid Respectively. 10% gelatin solution was added to the mixture, followed by pulverization and passed through a 14-mesh sieve. This was dried, to which 160 parts by weight of potato powder, 50 parts by weight of talc and 5 parts by weight of magnesium stearate were added, and the resulting mixture was purified.

&Lt; Formulation Example 3 > Preparation of a health food containing as a active ingredient an extract from Rhizoma root extract, Rhizoma root extract,

Foods containing as an active ingredient the Hymenoptera extract, Rhododendron rhizome extract, Sarcoma stem extract, Trichothecaster rhizome extract and saffron extract of Example 6 were prepared as follows.

<3-1> Production of flour food

0.5-5.0 parts by weight of a mixture of the hymenoptera extract, the rhizome root extract, the seedling stem extract, the hymenoptera extract and the saffron extract of Example 6 was added to wheat flour and the mixture was used to prepare a bread, a cake, a cookie, a cracker, Noodles were prepared.

<3-2> Manufacture of soups and gravies

0.1 to 5.0 parts by weight of a mixture of the rhizome root ending extract of Example 6, Rhizoma Rhizome Extract, Breeding Rhizoma Extract, Rhizoma Rhizome Extract and Saffron Extract were added to the soup and the juice to prepare health promotion meat product, noodle soup and juice .

<3-3> Preparation of ground beef

The ground beef for health promotion was prepared by adding 10 parts by weight of the mixture of the hymenoptera extract, the rhizome root extract, the seedling stem extract, the hymenoptera extract and the saffron extract of Example 6 described above to the ground beef.

<3-4> Manufacture of dairy products

5 to 10 parts by weight of a mixture of Hymenoptera, Rhizoma root extract, seedling stem extract, Tricholoma root extract, and saffron extract of Example 6 was added to milk, and the milk was used to make various dairy products such as butter and ice cream .

&Lt; 3-5 >

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The dried mixture obtained in Example 6 was concentrated under reduced pressure in a vacuum concentrator, dried with a spray dryer and a hot-air drier, and then dried with a granulator to a particle size of 60 mesh To obtain a dry powder.

The grains, seeds and the extract of Example 6 were mixed in the following ratios in the following proportions: extract of Rhizopus rhizome, extract of rhizome root, extract of Rhizoma stem, extract of Rhizoma root, and saffron extract.

(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

A mixture (3 parts by weight) of the raw material extract of Example 6,

(0.5 part by weight),

(0.5 parts by weight)

&Lt; Formulation Example 4 > Preparation of a beverage containing as an active ingredient a mixture of Rhodiola root extract, Rhododendron rhododendron extract, Stem stem extract, Rhododendron rhizome extract and saffron extract

<4-1> Production of health drinks

5 g of a mixture of a raw material such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75%) and the extract of Example 6 of the present invention Homogenously blended and sterilized instantly, and packaged in small containers such as glass bottles and plastic bottles.

<4-2> Production of vegetable juice

5 g of the mixture of the raw material extract of Example 6 was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice.

<4-3> Preparation of fruit juice

1 g of the mixture of the raw material extract of Example 6 was added to 1,000 ml of apple or grape juice to prepare fruit juice.

The composition of the present invention comprising collagen type I extract, collagen type I extract (collagen type I) which increases collagen biosynthesis in skin cells and increases collagen, which is a main component of the skin, 1), it is expected that it can be very usefully used in compositions for improving skin wrinkles and skin aging, for example, cosmetic compositions, pharmaceutical compositions, compositions for health foods, and external preparations for skin.

Claims (7)

A cosmetic composition for the improvement of skin wrinkles and skin aging, which comprises as an active ingredient, Hwangjung rhizome extract, Rhizoma root extract, Sargassum stem extract, Trichoderma root extract and saffron extract. The method according to claim 1,
The cosmetic composition for improving skin wrinkles and skin aging according to claim 1, wherein the extract is selected from the group consisting of Rhizopus rhizome extract, Rhododendron rhizome extract, Sarcoma stem extract, Trichoderma root extract and saffron extract at a weight ratio of 5: 1: 2.5: 0.5: The method according to claim 1,
Wherein the extract is obtained by extracting the extractant with an extraction solvent, and then filtering and concentrating the extract under reduced pressure, followed by drying and pulverizing the cosmetic composition. The method of claim 3,
Wherein the extracting solvent is water, alcohol or a mixture thereof. A pharmaceutical composition for improving skin wrinkles and preventing skin aging, which comprises as an active ingredient, Hwangjung rhizome extract, Rhizopus rhizome extract, Sarcoma stem extract, Trichomonic Rhizoma extract and saffron extract. A composition for a health food for the prevention of skin wrinkles and skin aging, which contains as an active ingredient an extract of Hwangjung rhizome extract, Rhizopus rhizome extract, a stem extract of Ganoderma lucidum, a Trichomoniasis extract and a saffron extract. A skin external preparation for skin wrinkle improvement and skin aging prevention containing an extract of Hwangjung rhizome extract, rhizome root extract, a stem extract for breeding, a trichome root extract and a saffron extract as an active ingredient.

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