KR20150120741A - Composition for preventing or improving atopic dermatitis comprising mixture of persimmon peel, persimmon flesh, persimmon leaf and Diospyros lotus leaf as effective component - Google Patents

Abstract

The present invention relates to a health functional food for preventing or alleviating atopic dermatitis and a pharmaceutical composition for preventing or treating atopic dermatitis, containing a mixture of a persimmon peel, a persimmon flesh, persimmon leaves and Diospyros lotus leaves as an active component. The healthy functional food and the pharmaceutical composition according to the present invention can: increase the availability for waste resources; provide commercial competitiveness; and exhibit enhanced effect in alleviating symptoms of atopic dermatitis to be usefully used industrially.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing or ameliorating atopic dermatitis comprising a mixture of persimmon peel, persimmon leaf, persimmon leaf and gomule leaf as an active ingredient. component}

The present invention relates to a composition for preventing or improving atopic dermatitis comprising a mixture of persimmon peel, persimmon leaf, persimmon leaf and gomoy leaf as an active ingredient. More particularly, the present invention relates to a composition for prevention or improvement of atopic dermatitis comprising a mixture of persimmon peel, persimmon leaf, And to a pharmaceutical composition for the prevention or treatment of atopic dermatitis and a health functional food for prevention or improvement of atopic dermatitis.

Edible and medicinally valuable persimmon trees ( Diosoyros kaki ) are classified into persimmon and sweet persimmon. Sweet persimmon trees are mainly cultivated in Japan. Sweet persimmon trees are mainly cultivated in Korea and China. The endemic persimmon, which is an endemic species cultivated in Korea, has been continuously improved in order to increase the value of persimmon. It is divided into three types, namely Gojong City, Qingdao Banshi, Danshi, Songgoshi, Danseong City, It is cultivated variously according to local temperature and soil characteristics. The Unjangsan area of Jeonan-gun and Wanju-gun, Jeollabuk-do are representative cultivars of Gojong-si of traditional varieties.

It is an alkaline fruit, rich in nutrients, rich in saccharides such as glucose and fructose, abundant in tannic acid which gives a pungent taste, and vitamin C is about 5 to 20 times higher than an apple of the same amount and rich in minerals. It is mainly used as a dried season (Hongshi) and dried persimmon except aged.

It is used mainly for dried persimmons, but it is also used as persimmon leaves because it can not be used for dried persimmons. Since persimmon is dried and circulated, it can be useful for restoring fatigue due to nutrients contained in persimmon and natural sugar. In addition, since persimmon shells are necessarily peeled off when manufacturing dried persimmon, persimmon shells are byproducts in the manufacture of dried persimmons. In the past, the shells were naturally dried to be used for snack streets. However, Most of them are abandoned and cause environmental pollution. Taking this into account, persimmon husk has recently been used in persimmon vinegar processing (Korean Patent, 10-2006-0082562) and powder tea manufacturing (Korean Patent, 10-2006-0082560). In terms of utilization of resources, byproducts are utilized in the production of livestock feed (Korean Patent, 10-2006-0082562). However, these processing methods are merely simple processing using persimmon shells and have limitations in their utility in creating high added value. Therefore, there is no development of processed food considering the functionality of persimmon husk.

The tannin and proanthocyanidin components contained in persimmon shells are water-soluble substances and have been known to have antibacterial, antioxidant, antitumor, and physiological activities such as removal of heavy metals, and have anti-obesity and arteriosclerosis prevention effects. In recent years, many researches have been carried out since the fact that the peeled polyphenol component has excellent antioxidant and antigenotoxic effect. In particular, carotenoids are abundant in persimmon peels, and proanthocyanidin among carotenoids has recently been shown to have an antidiabetic effect against type 2 diabetes, as well as antioxidative effects. However, there is no literature on the effect of persimmon on the relief of inflammation and itching in atopic dermatitis.

The physiological activities of antioxidants, antiinflammation and anti-itching of persimmon leaves are well known, and the processed products that can be applied to atopic dermatitis, which is most prevalent in children, are mainly related to cosmetic compositions (Korea Patent No. 10-2006-0099585) . Although it is widely known that fermentation is used to utilize the persimmon leaf extract, it is not only different in its usefulness depending on the strain used for fermentation, but also causes internal organs and skin toxicity due to germs acting as contaminants during fermentation, There are many. In particular, atopy is a skin disease that is very prevalent in children, and it should not be overlooked that the usefulness of unconditional fermentation can not be emphasized.

In addition, it has been widely used to attach persimmon leaf to the persimmon tree. However, recently, research on the physiological activity of the goom leaf tree has been conducted. Antioxidant, anti-hemolytic and anticancer activities are mainly reported in Goyom and its seeds, and only reports of anti-allergy and anti-itching are reported in Goyom leaf extracts.

Therefore, there is a need to make foods that are health-enhancing foods that children may prefer. In this respect, unlike processed products used in the past, the usefulness and manufacturing method of gamma-lattice containing persimmon leaves and gom- yoo leaves provided by the present inventors have the merit that they can differentiate from the existing application methods because they can sufficiently propose a new processing method have. In the present invention, a method for preparing a germanium composition capable of treating or improving atopic dermatitis by utilizing the nutritional components of persimmon leaves and the functions of persimmon leaves, gourd leaves and persimmon leaves, and the industrial applicability of the germanium compositions are presented.

Korean Patent Publication No. 2010-0035674 discloses a cosmetic composition for alleviating and preventing atopic dermatitis containing a fungus fermented persimmon leaf extract using medicinal mushroom microorganisms. Korean Patent Publication No. 2012-0077373 discloses a composition for preventing and treating atopic dermatitis, A composition for preventing or treating atopic dermatitis which comprises an extract of persimmon husk, persimmon leaf, persimmon leaf and gomoy leaf as an active ingredient according to the present invention, There is no description.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and the present inventors have made intensive studies to prepare a composition containing as an active ingredient persimmon leaf, gomoy leaf, persimmon leaf and persimmon shell, It was confirmed that there was an itching relieving effect and in a human body application experiment for children with atopic diseases in which a gamma latte containing the composition was prepared and consumed, the atlantic symptoms including the composition were significantly improved By confirming, the present invention has been completed.

In order to solve the above problems, the present invention provides a health functional food for prevention or improvement of atopic dermatitis comprising a mixture of persimmon husks, persimmon leaves, persimmon leaves and gomoy leaf as active ingredients.

The present invention also provides a pharmaceutical composition for preventing or treating atopic dermatitis, which comprises a mixture of persimmon peel, persimmon leaf, persimmon leaf and gomilla leaf as an active ingredient.

Of the materials used in the preparation of the composition of the present invention, persimmon husk, persimmon leaves and gomoyom except for persimmon were generally abandoned resources. Therefore, the composition for prevention or improvement of atopic dermatitis according to the present invention can be utilized industrially because it can increase the utilization of waste resources, has commercial competitiveness, and has a synergistic effect in improving atopic symptoms .

FIG. 1 is a photograph showing the skin lesion improvement effect before and after 28 days of treatment of the atorvastatin treated with gauze spores containing the persimmon leaves and the hot water extract solution of the gomule leaf, by oral administration of the final germatella composition of the present invention.

In order to accomplish the object of the present invention, the present invention provides a health functional food for preventing or improving atopic dermatitis comprising a mixture of persimmon peel, persimmon leaf, persimmon leaf and gomoy leaf as an active ingredient.

In the health functional food according to one embodiment of the present invention, the mixture is a mixture of the four active ingredients of persimmon peel, persimmon leaf, persimmon leaf and gomilla leaf, and compared to the effect of each ingredient to improve atopic dermatitis, The synergistic effect in the improvement of atopic dermatitis was shown in the case of the mixture of active ingredients (see Table 4 and Table 6). Therefore, the health functional food of the present invention essentially contains four components: persimmon peel, persimmon flesh, persimmon leaves, and gomule leaf.

The health functional food according to one embodiment of the present invention may be in the form of a powder mixture of persimmon, persimmon, persimmon leaf and gomule leaf, but is not limited thereto. These components may be a mixture of persimmon peel, persimmon leaf, persimmon leaf and talium leaf powder in a weight ratio of 74 to 81: 10 to 20: 2 to 6: 2 to 6, preferably 74 to 76: To 16: 4 to 6: 4 to 6, but the present invention is not limited thereto.

The health functional food of the present invention is not particularly limited as long as it can be ingested to prevent or ameliorate atopic dermatitis.

When the mixture of the persimmon peel, persimmon leaf, persimmon leaf and gomilla leaf of the present invention is used as a food additive, the mixture can be used as it is, or can be used together with other food or food ingredients, and can be suitably used according to a conventional method have. The amount of the active ingredient to be mixed can be suitably determined according to its intended use (prevention, health or therapeutic treatment). Generally, the mixture of the present invention is added in an amount of not more than 15 parts by weight, preferably not more than 10 parts by weight, based on the raw material, in the production of food or beverage. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of foods to which the above substances can be added include dairy products including meats, sausages, breads, chocolates, candies, snacks, confectionery, pizza, ramen noodles, gums, ice cream, soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include healthy foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.

The health beverage composition according to one embodiment of the present invention is a gamma latte. In addition to the mixture of persimmon husks, persimmon leaves, persimmon leaves, and gomoy leaves of the present invention, the present invention is also applicable to beverages such as skim milk powder, glucose, whole milk powder, alginic acid, guar gum, But the present invention is not limited thereto and can be prepared in a composition of 18 to 22, 11 to 15, 1 to 3, 0.1 to 1, 1 to 2, 0.1 to 1 and 0.1 to 1 in weight%.

In addition to the above, the mixture of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, A carbonating agent used in a carbonated beverage, and the like. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention also provides a pharmaceutical composition for preventing or treating atopic dermatitis, which comprises a mixture of persimmon peel, persimmon leaf, persimmon leaf and gomilla leaf as an active ingredient.

The pharmaceutical composition according to one embodiment of the present invention may be in the form of a powder mixture of persimmon, persimmon, persimmon leaf and gomule leaf, but is not limited thereto. These components may be a mixture of persimmon peel, persimmon leaf, persimmon leaf and talium leaf powder in a weight ratio of 74 to 81: 10 to 20: 2 to 6: 2 to 6, preferably 74 to 76: To 16: 4 to 6: 4 to 6, but the present invention is not limited thereto.

The pharmaceutical composition for preventing or treating atopic dermatitis according to the present invention may comprise 0.02 to 80% by weight, preferably 0.02 to 50% by weight, of the above mixture based on the total weight of the pharmaceutical composition.

Pharmaceutical compositions comprising a mixture of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions.

The pharmaceutical dosage forms of the mixtures of the present invention may also be used in the form of their pharmaceutically acceptable salts and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set.

The pharmaceutical composition comprising the mixture according to the present invention may be formulated in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups and aerosols, external preparations, suppositories and sterilized injection solutions, And can be used as formulations. Examples of carriers, excipients and diluents that can be included in the pharmaceutical composition containing the mixture include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium A variety of compounds or mixtures including silicates, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, Or lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao paper, laurin, glycerogelatin and the like.

The preferred dosage of the mixture of the present invention varies depending on the condition and the weight of the patient, the degree of the disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art. However, for the desired effect, the mixture of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably 0.001 to 100 mg / kg per day. The administration may be carried out once a day or divided into several times. The dose is not intended to limit the scope of the invention in any way. In addition, the composition according to the present invention may be administered alone, or may be administered together with other medicines in order to aid in the same or another medicament. In addition, in the composition of each formulation, components other than the composition, which is the above-mentioned essential ingredient, can be mixed and selected by a person skilled in the art according to the formulation or purpose of use of the other external preparation. In this case, Can happen.

The mixtures of the present invention can be administered to mammals such as rats, mice, livestock, humans, and the like in a variety of routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

Hereinafter, the present invention will be described in detail with reference to examples. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

Materials and methods

1. Preparation of dried persimmon leaves and goat leaves

The persimmon leaves used in the experiment ( Diospyros kaki leaf) and gourd leaf ( Diospyros lotus leaf was collected from 20 to 30 days in June 2013 at Gamdong village, Jungcheon - myeon, Jinan - gun, Jeollabuk - do. The identification of persimmon leaves and gomoyob leaves was confirmed by the residents of Gojong-si and Goyom trees, and finally confirmed by professor Kim Hong-joon of Woosuk University Oriental medicine preschool classroom. (# 2013-DK0620) and Guillaume Leaf Specimen (# 2013-DL0620) are kept in the Health Management Laboratory of the College of Alternative Medicine at Jeonju University. The collected persimmon leaves and gyom leaves were immediately washed with distilled water, steamed for 5 minutes in order to remove germs and contaminants, and dried in a dryer at 37 to 40 ° C. for 12 hours to adjust the moisture content to 10% or less. Dried persimmon leaves and gomoyle leaves were used for the preparation of ultrafine powders.

For the experiment to improve atopic dermatitis, hot water extract of persimmon leaf and gomoy leaf was prepared. The dried persimmon leaf and gomoy leaf were pulverized to 500 g, 5,000 ml of distilled water was added, boiled for 10 minutes, Filter. ≪ / RTI >

2. Aging and drying persimmon and skin

Fresh persimmon ginseng (persimmon ginseng) used for dried persimmons was collected from November 10-20, 2013 at Gamdong village, Jungcheon - myeon, Jinan - gun, Jeollabuk - do. After the skin was peeled off, the pulp and the skin were aged for 10-20 days in the shade, dried in a shady natural state and finally dried in a dryer at 37-40 ° C for 12-72 hours to obtain a water content of 7% (# 2013-DKP 1210, # 2013-DKP 1211).

3. Manufacture of persimmon leaves, gomoyo leaf, persimmon nut powder

First, the sufficiently dried persimmon leaves, gomoyobu leaf, and persimmon shells were prepared as fine 200-mesh powders using a pulverizer (DW-400L, KARI Industry Co., Ltd.).

4. Optimal conditions for producing ultrafine powder of persimmon

In general, persimmon pulp was dried using an infrared drier to sufficiently dry (moisture: less than 10%). However, dried pulpy flesh has a problem in producing ultrafine powder (100-500 mesh) because it contains a large amount of sugar (reducing sugar: 200 mg / g / g; Brick: 56.40). For example, In addition, due to moisture, even in the natural state, it coagulates like sugar and has many problems in ultrafine powder formation. Therefore, in order to obtain an optimum fine powder, the present invention was made by adding other main components as follows to obtain an optimum composition.

① Problems of ultrafine powdering due to high sugar content of dried persimmon

The sugar content of aged persimmon is 12-14 bricks, whereas the dried persimmon is 50-60 bricks. Because of its high sugar content, it can be pulverized to 100-200 mesh even when it is fully dried. However, powdered ultrafine powder Has a problem in pulverizing dried persimmon because it becomes solid due to entanglement.

② Optimal condition Derived guttae composition

The present invention relates to a gum tragacanth composition utilizing the functionality of a persimmon tree, and is produced by applying the following conditions to ultrafine pulverization of persimmon flesh using sufficiently dried persimmon leaves, gumum leaves and persimmon leaves.

Dried persimmon or persimmon mono-finely powdered division ratio(%) Powder size (mesh) Characteristic 100 200 500 Persimmon 100 possible possible possible 100-500 mesh super fine powdering possible
[Advantages] Nanoparticles possible, raw material price cheaper
[Disadvantages] When preparing latte, high sugar content is required from monosaccharide to oligosaccharide Persimmon 100 possible impossible impossible 100 mesh is possible but solidification immediately
[Advantages] In the production of latte, natural sugar can be utilized
[Disadvantages] It is solidified immediately after being powdered and is not suitable for use as a raw material for latte,

Mixing ratio of dry persimmon and persimmon for ultrafine powdering Compound content
(Total 100g) Powder size (mesh) Characteristic Remarks 100 200 500 Peel (50 g) + pulp (50 g) possible impossible impossible 100 mesh particles are possible but particles are thick and precipitate
Solidification during long-term storage Peel (70g) + pulp (30g) possible possible impossible 100-200 mesh is possible but solidification immediately
[Advantages] Natural sugar can be used in latte production
[Disadvantages] It is not suitable to use as a raw material of latte composition because it solidifies immediately after powdering Peel (80g) + pulp (20g) possible possible possible Available in 100-500 mesh
[Advantages] Natural sugar can be used in latte production
[Cons] 500 mesh partial solidification optimal Peel (90g) + flesh (10g) possible possible possible Available in 100-500 mesh
[Advantages] Natural sugar can be used in latte production
No solidification phenomenon in all particles optimal

○ Percentage by weight of dry persimmon and persimmon for ultrafine powdering

- 50-100 g of crust husk part, 10-50 g of crumb pulp weight part, preferably 80-90 g of dry crust crumb and 10-20 g of crumb pulp weight are mixed to prepare ultrafine powder

○ Percentage by weight of dry persimmon, dry persimmon, dried persimmon leaves and dry goose leaves for ultrafine powdering

50 to 100 g of crusts, 10 to 50 g of crumbs, 10 to 50 g of persimmon leaves, 1 to 30 g of guormwood leaves, preferably 80 to 90 g of dried crusts, 10 to 20 g of the weight of the pulp, 1 to 5 g of the weight of the persimmon leaf, and 1 to 5 g of the gomace leaf are mixed to prepare ultrafine powder

○ Percentage of dry persimmon, dry persimmon, dried persimmon leaves, dry goose leaves and other excipients for ultrafine powdering

50 to 100 g of crusts, 10 to 50 g of crumbs, 10 to 50 g of persimmon leaves, 1 to 30 g of guormwood leaves, preferably 80 to 90 g of dried crusts, Weight of fragrant leaf, 1-5 g of persimmon leaf, 1-5 g of goatee leaf and other excipients (including but not limited to whole milk powder, skim milk powder, glucose, fructose, sugar, etc.) And the like are mixed to prepare ultrafine powder

5. Treatment of atopy with persimmon leaves, gourd leaves and persimmon leaves

Process for treating atopy containing persimmon leaves, gourd leaves and persimmon leaves Manufacturing sequence Manufacturing content Characteristic 1. Material preparation (cleaning) Persimmon leaf Persimmon leaves and gourd leaves are thoroughly washed with water to remove contaminants Wash Wash thoroughly to remove contaminants
- Persimmon shell: Preparing the shell using a machine for persimmons
- Persimmon pulp: Using a knife, 2. Material preparation (drying) Persimmon leaves and gourd leaves are steamed and dried - The washed persimmon leaves and gomoyle leaf are steamed for about 5 minutes to remove the pathogenic microorganisms and remove the cuticle layer and chlorophyll of leaves.
- After that, it is dried with a moisture content of 10% Dried persimmon and persimmon after aging - Persimmon peel and persimmon are shade, aged about 10-20 days dry
- Then use an infrared drier to dry to a moisture content of less than 10% 3. Mixing of raw materials Persimmon peel, Persimmon flesh, Persimmon leaf, Gomilla leaf blend - Apply the above mixing ratio 4. Ultrafine grinding Ultra-fine powder utilization grinding - Particle size: 100-500 mesh 5. Final Latte Composition Final latte composition blending
- Main ingredient: persimmon peel, persimmon flesh, persimmon leaves, gomilla leaf blended ultrafine powder
- Ingredients: alginic acid, glucose, oligosaccharide, skim milk powder, whole milk powder, guar gum, xanthan gum, milky incense etc. The final composition is referred to in the following section 6. Filling Pouch charging 7. Packing Packing

6. Final composition of gamata

The compositions of persimmon peel, persimmon leaves, persimmon leaves and goat leaves were prepared as shown in Table 4 below, and the effect of this composition on the reduction of the itching and vascular permeability was examined in mice. That is, the itch, which is a major symptom of atopy, is a major cause of disruption of the skin barrier, and therefore, a composition prepared from the main components of the gherkin was tested. The compound 48/80 used in the experiments was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Persimmon leaves, persimmon leaves, persimmon leaves and persimmon leaves Raw material content(%) Normal control group Control group A composition B composition C composition D composition Persimmon - - 85 83 80 75 Persimmon - - 15 15 15 15 Persimmon leaf - - 0 One 2.5 5 Guillaume leaves - - 0 One 2.5 5 Sum 0 0 100 100 100 100

[Normal control group: only feed without composition

Control group: only feed without composition + itching inducer compound 48/80 injection

A composition: composition without persimmon leaf and gomoma leaf + compound 48/80 injection

BD composition: composition of persimmon leaf and gomex leaf + compound 48/80 injection]

6-1. Experimental animal effect experiment

① Experimental animals

Seven week old male Balb / c mice raised in sterile environment were purchased from the Central Laboratory Animals (Korea) and used for experiments for one week while feeding with sufficient feed and water. The temperature and the humidity (50-60%) were kept constant at the temperature (20-22 ℃) and 12 hours at the day and night cycle, respectively. According to the provisions of the Laboratory Animal Commission (Jeonju University) Experiments were conducted.

② Composition treatment and itching experiment

Balb / c mice with stress-relieved mice were placed in a transparent acrylic cage (20x26x13 cm) each of 5 mice per experimental group and left in the same experimental environment for 30 minutes for stability. Then, physiological saline was orally administered to the control group, and the compositions (A, B, C, and D) were orally administered to the mice at 500 mg per kg of body weight twice a day for 3 days. / Kg, mouse body weight) was orally administered 60 minutes before, and 100 μl of compound 48/80 (50 μg / site), which is an itch inducer, was injected subcutaneously at the level between both shoulders such as a mouse.

③ Itching test

The mice injected with the itch inducer were immediately recorded for 60 minutes using a micro camera (ONCCTV, Korea) according to the method of Mihara ( Br . J. Dermato . 2004, 151 : 335-345) The number of scraping of the site where the itching inducing substance was injected was evaluated by counting by the double blind method. Experiments on each inducer were conducted on different days and the mice used in each experiment were used once.

④ Vessel penetration

Evans blue (Evans blue) was injected subcutaneously in the same site immediately after administering the composition as described above, and the injected material was treated immediately. After 60 minutes, the injected skin (10x10 mm) was removed and 1 M potassium hydroxide And left to stand for 12 hours, 4 ml of a 0.2 M phosphoric acid-acetone (5:13) solution was added. Thereafter, the solution was vigorously stirred, filtered, and the absorbance of the filtered solution was measured at 620 nm to measure the vascular permeability.

6-2. Assessment of body composition

Animal experiments were conducted to determine the effects of a mixture of the main components of the gherkin (persimmon skin + persimmon leaf + persimmon leaf + gomule leaf), and then applied to human body as shown in Table 6 below. And atopic dermatitis patients.

The weight ratios of the preferred germail compositions are as shown in Table 5. The weight ratios applicable to the human body are 30-70 g of gamma-starch mixture (persimmon + persimmon + persimmon leaves + gomule leaf), 20-30 g of skim milk powder, 3-5 g of powdered milk, 1-2 g of alginic acid, 0.5-2 g of guar gum, 0.2-1 g of xanthan gum and 0.5-2 g of milk fractions.

The final composition of gamma ray for human body sensory evaluation Raw material content(%) Persimmon 50 Persimmon 7 Persimmon leaf 2.5 Guillaume leaves 2.5 Skim milk powder 20 Glucose 13 Whole milk powder 2 Alginic acid 0.5 Guar gum 1.5 Xanthan gum 0.5 Milk flavor 0.5 Sum 100

[Comparative Composition 1: Except for the persimmon leaf and dried gomilla leaf powder, all of the other ingredients are formed in the same ratio as in Table 5

Comparative Composition 2: Composition in which the persimmon leaves and the gomule leaf dry powder were respectively 1% and 2% reduced in the compositions of Table 5

Comparative Composition 3: Composition in which the persimmon leaf and the gomule leaf dry powder were respectively 5% and 10% reduced in the composition of Table 5,

① Sensory evaluation

Sensory evaluation was performed on the germano composition prepared as shown in Table 5 by dividing into 20 groups (10 males and 10 females) and 20 atopic children (10 males and 10 females) healthy smelling and taste buds Respectively. The sensory evaluation method is based on the criteria (1 point: very bad, 2 points: bad, 3 points: normal, 4 points: good, 5 points: very good) for taste, flavor, texture and solubility The scores were given as average values.

② Experimental effect of atopic dermatitis

A total of 30 healthy children (2-10 years old) and atopy children (10 boys and 20 girls: 2-10 years old) were divided into two groups as follows (morning and evening) for 28 days: Gauze spores (gauze: 5 layers of 5x5 cm in size, solution amount of 10 ml) containing orally administered with gherkin and persimmon leaves and hot water extract of gomoy leaf were treated at the same time.

The selection of children with atopic dermatitis was conducted in a dermatologic hospital through a comprehensive evaluation of itching, edema, erythema and bleeding, scratching and erosion,

In order to evaluate the improvement effect of the germatella composition of the present invention on atopic dermatitis lesion sites, the results were evaluated at 2 weeks and 4 weeks after SCORAD (SCORAD: SCORing Atopic Dermatitis) measurement.

Example 1. Analysis of the effects of reducing the itching and reducing the blood-penetration power of the mixture of the main ingredient of gametta

As a result of analyzing the effects of the testosterone and vascular permeability of the germane active ingredient (persimmon peel + persimmon leaf + persimmon leaf + gomoy leaf) of Table 4 in the animal test, as shown in Table 6, And the BD composition containing the persimmon and persimmon leaves alone and the BD composition supplemented with persimmon leaves and gomoyle leaves had a significant effect on the itching relief and the BD composition containing the persimmon leaves and gomoy leaf alone had a significant effect, Compared with the A composition containing only the crustacea and the persimmon extract, the effect of increasing the amount of itching was increased as the addition amount was increased. In addition, the B-D composition with persimmon leaves and gonilla leaves showed an increased effect on the itching relief as the amount of persimmon leaf and gomoy leaf was increased, as compared with the composition containing only persimmon leaf and persimmon leaf, as well as vascular permeability.

Reduction of itching and vascular permeability reduction of mixture division Normal control group Control group A composition B composition C composition D composition 60-minute cumulative scratches 10.5 ± 1.3 230.3 ± 20.2 ^# 175.2 ± 18.5 ^* 115.7 ± 21.9 ^** 85.5 ± 9.8 ^*** 75.3 ± 10.4 ^*** Vessel penetration (%) 18.7 ± 1.2 100.1 + - 8.1 ^# 81.2 ± 9.2 ^* 51.2 ± 7.5 ^** 38.2 ± 3.9 ^*** 34.8 ± 5.6 ^***

^# P <0.001: Comparison with normal control group

^* P <0.05, ^** P <0.01, ^*** P <0.001: compared with control group

Example 2. Analysis of the improvement effect of atlantic dermatitis symptom on ghattila mixture

As a result of the sensory evaluation performed on the gum latratch composition prepared as shown in Table 5, the gum latte composition maintained taste, flavor, texture and solubility as in comparative composition 1, as shown in Table 7 below. However, the comparative composition 3 had poor taste, flavor, texture, and solubility as compared to the germ latrines and comparative compositions 1 and 2 of the present invention.

Sensory Evaluation of the Final Gametta Composition division flavor zest Texture Solubility (releasability) Gherkin composition 4.6 4.5 4.7 4.3 Comparative Composition 1 4.7 4.6 4.8 4.6 Comparative Composition 2 4.6 4.5 4.7 4.5 Comparative Composition 3 3.6 3.9 3.8 3.5

(1 point: very bad, 2 points: bad, 3 points: normal, 4 points: good, 5 points: very good)

Based on the sensory evaluation results, the skin-improving effect of atopic children was examined in a ganoderma composition containing 2.5% each of persimmon leaves and gourd leaf powder as shown in Table 5 above. Experiments were performed in groups as follows.

○ healthy children

- Final ghatti (Table 5) Intake comparison group: 5 healthy children

- Final Gamert (Table 5) ingestion + gauze sprouting Comparative group: 5 healthy children

○ Atopy children

- Gauze spore treatment Comparison group: Atopy children 10

- Final ghatti (Table 5) Intake comparison group: Atopy children 10

- Final Gamert (Table 5) ingestion + gauze sprouting Comparative group: Atopy children 10

In the first study, the changes of the skin of the ghattache composition alone group, the ghoul latte composition intake and the gauze sprout treatment group were observed in the healthy children comparison group. As a result, no skin reaction was observed in the final ghatti intake or final ghatti intake and gauze sprouting group No skin toxicity was observed for this composition.

After confirming that the germano composition used in the present invention was free from skin toxicity, the improvement effect of the germano composition on atopic dermatitis lesion sites was confirmed after 2 and 4 weeks of treatment using the SCORAD measurement method, The results are shown in Table 8 below.

<Criteria>

○ Extent criteria: area = damaged area / 100

○ intensity criteria:

- Erythema (1/2/3)

- Edema (1/2/3)

- Excretion (1/2/3)

- Skin exfoliation (1/2/3)

- Degree of firing (1/2/3)

- Degree of drying (1/2/3)

○ Subjective criteria:

- Itching (1-10)

- Insomnia (1-10)

○ Scored calculation = (precision standard / 5) + (7 / strength standard) + subjective standard

Atopic dermatitis symptom improvement effect Classification 0 weeks 2 weeks 4 weeks Example 1
(gauze) Scofield 61.8 42.5 36.7 Degree of improvement 0% 31.2% 40.6% Example 2
(Intake) Scofield 68.1 34.5 24.7 Degree of improvement 0% 49.3% 63.7% Example 3
(Intake + gauze) Scofield 67.6 27.3 11.3 Degree of improvement 0% 59.6% 83.3%

As shown in Table 8, the skin improvement effect was good in Example 2 in which the final germatella composition was orally administered (taken), rather than Example 1 in which the hot-water extract of persimmon leaves and gomoyom leaves was treated with gauze sprouts. In particular, in Example 3 in which the final germane and gauze spores were simultaneously treated, it was confirmed that the symptoms of atopic symptoms were significantly improved as compared with those of the examples treated separately. These results demonstrate that it is preferable to use both the food to be consumed and the external preparation to be applied, since the skin of the integumentary system appears to be a lesion due to the nature of atopic dermatitis. Typically, the atopic dermatitis symptom improvement effect of atopic children is shown in Fig.

Therefore, considering that the composition shown in the present invention can be used to improve atopic dermatitis very usefully, and the atopic dermatitis is explosively increased (the prevalence of atopy in industrialized countries: 17-25%), It is also considered to be a very useful and popular composition that can be applied to people who are sensitive to sensitive skin including healthy people. In particular, ghermatte is considered to be a very ideal drink considering its rich flavor and taste and its abundance of nutritional and functional ingredients.

Claims (7)

A health functional food for prevention or improvement of atopic dermatitis containing a mixture of persimmon peel, persimmon leaf, persimmon leaf and gomilla leaf as an active ingredient. The health functional food for preventing or ameliorating atopic dermatitis according to claim 1, wherein the mixture is a mixture of persimmon peel, persimmon flesh, persimmon leaf and gomoy leaf. The health functional food for preventing or improving atopic dermatitis according to claim 2, wherein the powder mixture is an ultrafine powder mixture of 100 to 500 mesh. 3. The method according to claim 2, wherein the mixture is a mixture of persimmon peel, persimmon flesh, persimmon leaves and talium leaf powder in a weight ratio of 74 to 81: 10 to 20: 2 to 6: 2 to 6 Health functional foods for improvement. The health functional food for prevention or improvement of atopic dermatitis according to claim 1, wherein the health functional food is a kumquat. [Claim 5] The method of claim 5, wherein the gamma latte comprises a mixture of persimmon peel, persimmon leaf, persimmon leaf and gomoyle leaf as an active ingredient and additionally contains skimmed milk powder, glucose, whole milk powder, alginic acid, guar gum, xanthan gum, Or a pharmaceutically acceptable salt thereof, for the prevention or amelioration of atopic dermatitis. A pharmaceutical composition for the prevention or treatment of atopic dermatitis comprising a mixture of persimmon husk, persimmon leaf, persimmon leaf and gomilla leaf as an active ingredient.

Images