JP2013203683A - Type iii collagen production promotor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a type III collagen production promotor capable of effectively promoting production of type III collagen serving as a constitutional component of derma, being continuously orally ingestible, and including a highly safe active ingredient derived naturally.SOLUTION: A type III collagen production promotor includes herbal medicines or extracts thereof consisting of stems or leaves of Astragalus membranaceus, ginseng, licorice, Leguminosae Dolichos lablab L., Coix lachrymal-jobi var. ma-yuen, malt, Zingiberaceae Amomum cardamomum L., Crataegus cuneata and Aurantii nobilis pericarpium. As to the herbal medicines or the extracts thereof, content ratios of ingredients are: 2.0 of the stems or leaves of Astragalus membranaceus; 1.0 of the ginseng; 1.0 of the licorice; 0.6 of the Leguminosae Dolichos lablab L.; 0.4 of the Coix lachrymal-jobi var. ma-yuen; 0.4 of the malt; 0.4 of the Zingiberaceae Amomum cardamomum L.; 0.2 of the Crataegus cuneata; and 0.1 of the Aurantii nobilis pericarpium.

Description

  The present invention relates to a production promoter for type III collagen, which is one of extracellular matrix components.

  One of the causes of skin wrinkles and sagging due to aging is a decrease in skin elasticity (flexibility). Human skin is composed of the stratum corneum, epidermis layer, basement membrane, and dermis, which occupies the widest area. It is known that type I and type III collagen (about 80% and about 15%, respectively), which are abundant in the dermis, decrease as fibroblast function declines due to aging. It is thought that the elasticity (flexibility) is lost and wrinkles and sagging occur.

  In particular, type III collagen is said to have a function of imparting flexibility, but adult tissues have a much lower proportion of type III collagen in the dermis than infant tissues and tend to decrease rapidly with age. It is known that

  In addition, Ehlers-Danlos syndrome, a clinical symptom caused by abnormalities in the mechanism of collagen fibril formation, is a type of skin symptom where wrinkles are conspicuous in the skin, causing bleeding with very weak external force. Although it is easy and there is a risk of causing symptoms such as arterial rupture and intestinal perforation, these diseases are considered to be caused by a synthesis failure of type III collagen (Non-patent Document 1).

  Thus, type III collagen is a component essential for building normal and soft skin and suppressing skin aging due to aging, and can improve various diseases caused by type III collagen synthesis failure. It can be said that it is an important ingredient. Therefore, it is desired to develop a type III collagen production promoter that can effectively promote the production of type III collagen and that contains a naturally occurring and highly safe active ingredient that can be continuously ingested.

JP 2001-039849 A JP 2006-3105050 A

Collagen metabolism and disease, page 60, Kodansha (1989)

  It is an object of the present invention to effectively promote the production of type III collagen, which is a constituent component of the dermis, and to combine type III collagen with a naturally derived and highly safe active ingredient that can be continuously ingested. It is to provide a production promoter.

  As a result of examining the action of promoting the production of type III collagen of human skin-derived fibroblasts for various substances in order to solve the above problems, the present inventor has found that stems or leaves of ogi, carrots, licorice, hens, pearl barley, bakakuga The present invention has been completed by finding that a herbal medicine composed of peanut, peanut, hawthorn and chimpi or an extract thereof exhibits an excellent action.

  That is, the present invention is a type III collagen production promoter characterized by containing a crude drug consisting of an oat stem or leaf, carrot, licorice, hens, pearl barley, bakuga, peacock, hawthorn and chimpi or an extract thereof. .

  Furthermore, the present invention relates to a herbal medicine or an extract thereof comprising an oat stalk or leaf, carrot, licorice, hens, pearl barley, bakuga, peacock, hawthorn and chimpi, an oat stalk or leaf 2.0, carrot 1.0, Type III collagen production promoter characterized by being blended in a ratio of licorice 1.0, hens 0.6, pearl barley 0.4, bakuga 0.4, peanut 0.4, hawthorn 0.2, and chimney 0.1 It is.

  According to the type III collagen production promoter of the present invention, type III collagen production by fibroblasts can be promoted, and skin aging due to aging can be effectively suppressed.

  In addition, by promoting type III collagen production, type III such as arterial collapse and intestinal perforation classified as clinical symptoms type IV of Ehlers-Danlos syndrome caused by abnormalities in collagen fiber formation mechanism Various diseases caused by collagen synthesis failure can be improved.

Graph showing changes in type III collagen production

  The type III collagen production-promoting agent of the present invention contains herbal medicines or extracts thereof, which consist of barley stems or leaves, carrots, licorice, hens, pearl barley, bark moths, peanuts, hawthorn, and chimpi.

  Commonly known ogi (yellow japonicus) is a product of Leguminosae that is included in the refined `` Shinnohonkaku '' and dried roots of Astragalus membranaceus. It is widely used as a traditional Chinese medicine that has the effect of removing tonic and water surface poisoning. However, these effects are mainly caused by the roots of the ogi (jaundice), and there are no reports on the medicinal effects on the stems or leaves of the ogi (jaundice), and few are known. The type III collagen production promoter of the present invention promotes type III collagen production by combining stems or leaves of ogi (yellow jade), or extracts thereof, and other plant components.

  From the viewpoints of usability, formulation, etc., it is preferable to use herbal medicine as a dry powder or extract for the stems or leaves of ogi (yellow jade).

  As a method for obtaining a dry powder, there are a method in which a crude drug is chopped or pulverized and then dried, and a method in which a crude drug is dried and then chopped or pulverized to obtain a dry powder.

  The extraction method of the extract used in the present invention can be performed by solvent extraction. In the case of solvent extraction, all of the herbal medicine or various parts (stems and leaves in the case of ogi) are dried as necessary, and chopped or crushed as necessary, and then an aqueous extractant such as cold water, hot water, Alternatively, it is extracted by using hot water having a boiling point or lower temperature, or an organic solvent such as methanol, ethanol, 1,3-butanediol, ether or the like at normal temperature or by heating. However, the extraction method is not limited to solvent extraction, and may be a conventional method known in the art. The form of the extract may be not only the extract itself, but may be appropriately diluted or concentrated by a conventional technique, and is a powdery or massive solid obtained by drying the extract. Also good.

  Carrot (carrot) is a carrot (carrot) that has been well-known as a herbal medicine, and is used for patients with weak symptoms of gastrointestinal function such as pain in the upper abdomen, lack of appetite, diarrhea, and vomiting. It has been. The pharmacological effects of carrots (carrots) include nervous system excitability, pituitary-adrenocortical excitability, sexual function enhancement, cardiotonic activity, blood glucose lowering activity, hypercholesterolemia occurrence suppression, blood Medium cholesterol lowering action, antidiuretic action, antianaphylaxis action, etc. are known.

  Licorice (licorice) is a product obtained by drying the roots of leguminous perennials, and contains sweet components such as glycyrrhizin, glucose and sucrose. Glycyrrhizin is well known because its degradation product binds to harmful products in the liver of the living body and detoxifies.

  Hens (dried bean) is a dried legumeinosae Dolichos lablab L. (Fuji bean) seeds, and in Kampo, in the summer, gastrointestinal cold, acute gastroenteritis, indigestion, etc. It is used when there are symptoms of heat and humidity such as dry mouth, wanting to drink water, upper abdomen passage, vomiting, diarrhea, anorexia.

  The pearl barley is called cedar (coix lachrymal-jobi var. Ma-yuen, Coix lacryma-jobi L. var. Frumentacea Makino or Coix ma-yuen) in Chinese medicine. Because of its diuretic, analgesic, antispasmodic and tonic effects, it is used for edema, neuralgia, bladder stones, etc.

  Bakuga (malt) is known to have an action of healthy stomach and lactation (considered to mean elimination of milk secretion and reabsorption of milk) in Chinese medicine, and is used as a herbal medicine.

  Byakuzuku (white bean pod) is a dried seed of the fruit of Zingiberaceae white bean pod Amomum cardamomum L. In Chinese medicine, it is an acute gastritis that occurs after cold exposure or violent overdose. Use when taking painful symptoms such as nausea, vomiting, and abdominal pain.

  Hawthorn It is dried and has known digestion promoting action, antibacterial action, and vasodilatory / hypertensive action.

  Chinpi is a dried ripe mandarin peel and is used for cough with a lot of sputum in Chinese medicine.

  When the type III collagen production promoter of the present invention is orally ingested (administered), additives can be arbitrarily selected and used as necessary. As additives, functional materials, excipients, and flavoring agents can be included.

  Functional materials include various vitamins such as pantothenic acid, folic acid and biotin, various minerals such as zinc, calcium, magnesium and iron, amino acids, oligosaccharides, propolis, royal jelly, ginkgo biloba, turmeric, EPA, DHA, coenzyme Q10, Chondroitin, lactic acid bacteria, lactoferrin, isoflavone, prunes, chitin, chitosan, glucosamine, α-lipoic acid, agaricus, garcinia, propolis, collagen, astaxanthin, forthrin, catechin, sesamin, ceramide, moroheiya, spirulina, cats claw, etc. . These functional materials can be used alone or in combination of two or more.

  The excipient may be anything that is usually used when it is in the desired dosage form, for example, starches such as wheat starch, rice starch, corn starch, potato starch, dextrin, cyclodextrin, and crystalline celluloses. , Sugars such as lactose, glucose, sugar, reduced maltose, starch syrup, fructooligosaccharide, emulsified oligosaccharide, and sugar alcohols such as sorbitol, erythritol, xylitol, lactitol, mannitol. These excipients can be used alone or in combination of two or more.

  As a flavoring agent, various fruit juice extracts such as Bontang extract, lychee extract and yuzu extract, various fruit juices such as apple juice, orange juice and lemon juice, various flavors such as peach flavor, ume flavor and yogurt flavor, acesulfame K, sucralose , Erythritol, oligosaccharides, mannose, xylitol, isomerized sugars, and other sweeteners, citric acid, malic acid, tartaric acid, gluconic acid, and other acidulants, green tea, oolong tea, banaba tea, Tochu tea, iron kannon tea, pearl barley Various tea ingredients such as tea, amacha mul tea, macomo tea, and kelp tea are listed. These taste agents can be used alone or in combination of two or more.

  For other colorants, preservatives, thickeners, binders, disintegrants, dispersants, stabilizers, gelling agents, antioxidants, surfactants, preservatives, pH adjusters, etc. The known ones used in the preparation process such as can be appropriately selected and blended.

  The dosage form of the type III collagen production promoter can be arbitrarily selected from, for example, liquid, solid, granule, granular, paste, and gel.

  The type III collagen production promoter of the present invention can be widely applied to cosmetics, quasi-drugs and the like applied to the outer skin, and the dosage form is also an aqueous solution system, a solubilization system, an emulsification system, and a powder. A wide range of dosage forms such as a system, an oil liquid system, a gel system, an ointment system, an aerosol system, a water-oil two-layer system, and a water-oil-powder three-layer system can be adopted. And if it is a basic cosmetic, said various dosage forms can be widely applied to forms, such as a lotion, a milky lotion, a cream, a gel, an essence (beauty liquid), a pack, a mask. Moreover, if it is makeup cosmetics, it can apply widely to forms, such as a foundation, and if it is a quasi-drug, it can apply widely to forms, such as various ointments. However, the form that the type III collagen production promoter of the present invention can take is not limited to these dosage forms and forms.

  When applied to the outer skin, components usually used in skin external preparations such as cosmetics and quasi-drugs, such as whitening agents, moisturizers, antioxidants, oily components, ultraviolet absorbers, surfactants, thickeners , Alcohols, powder components, coloring materials, aqueous components, water, various skin nutrients, and the like can be appropriately blended as necessary.

  In addition, as other components, edetate disodium, edetate trisodium, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid and other metal sequestering agents, caffeine, tannin, verapamil, tranexamic acid and its derivatives, Licorice extract, glabrizine, hot water extract of karin fruit, various herbal medicines, tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, kojic acid Other whitening agents such as glucose, fructose, mannose, sucrose, saccharides such as trehalose, and the like can be appropriately blended.

  Hereinafter, the confirmation test of the effect performed about the type III collagen production promoter of this invention is described as an Example.

The herbal extract used in the present invention was extracted using the following method.
"Extraction method"
Nine kinds of herbal medicines were mixed in the following ratios, followed by hot water extraction (about 90 ° C.) for 60 minutes with 10 times the amount of water. After mesh filtration, the residue was extracted and filtered again under the same conditions.
The obtained filtrates were combined and concentrated under reduced pressure. After filtration, the filtrate was dried to obtain an extract.
In addition, it is not restricted to the method of obtaining an extract from 9 types of mixed crude drugs like an Example, For example, a desired extract is obtained also by the method of mix | blending the extract obtained for every crude drug with the following ratio. Can do.
Mixing ratio 1. Ogi Leaf 2.0
2. Carrot 1.0
3. Daylily 1.0
4). Hens 0.6
5. Pearl barley 0.4
6). Bakuga 0.4
7). Byakuzuku 0.4
8). Hawthorn 0.2
9. Chimpi 0.1

"Sample preparation"
A test sample obtained by diluting the extract (dried product) to a predetermined concentration (final concentration: 0.005 and 0.01% by mass) was used for the culture test.

"Test method"
Test Method for Promoting Type III Collagen Production (1) Culture of Human Skin Fibroblasts Human skin fibroblasts cultured in 10% FBS-containing DMEM medium were seeded in a 24-well plate and the cells adhered, then 0.25 The test sample was added after replacing with DMEM medium containing% FBS and 250 μM magnesium ascorbate phosphate. Three days later, the culture supernatant was collected and centrifuged, and the type III collagen in the obtained supernatant was measured, and the amount of DNA was measured for the cells, which was used as an indicator of the number of cells.
(2) DNA quantification The amount of DNA was measured by a fluorescence measurement method using Hoechst H33342.
(3) Measurement of type III collagen The ability of cells to biosynthesize type III collagen is determined by measuring the amount of terminal peptide of type III procollagen (Procollagen type III-peptide: abbreviated as PIIIP) secreted into the culture supernatant. It was evaluated by. The measurement was performed using a commercially available product “Riagnost PIIIP measurement kit (manufactured by CIS Bio International)”. The production amount of type III collagen was divided by the amount of DNA, and evaluated by a relative value with respect to a sample to which no plant extract was added (control).

  In addition, statistical processing was performed to verify whether there was a difference based on the collected samples. If the significance (p) indicated by the test result is less than 5% (p <0.05), there is a significant difference between the data items (referred to as variables) used in the test. A significant difference means that a difference has occurred for some reason. When conducting a biological test, whether or not there is a significant difference in the end is a technique for deriving whether there is a change between groups. The test was performed by t test.

"Test results"
FIG. 1 is a graph showing the change in the amount of type III collagen production (%) per DNA relative to the non-added control (control). Both test samples with added concentrations of 0.005 mass% and 0.01 mass% increased type III collagen production with respect to the control (control), and a significant difference was observed (added concentration of 0.005 mass% was p < 0.05, p <0.001 for added concentration of 0.01% by mass. In addition, as shown in the graph, the higher the concentration of the extract contained, the higher the amount of type III collagen produced, and nine types of herbal extracts effectively promote type III collagen production. It was confirmed that there was an effect.

Claims (2)

  A type III collagen production promoter characterized by containing a crude drug consisting of an oat stem or leaf, carrot, licorice, hens, pearl barley, peony, sand crab, hawthorn and chimpi or an extract thereof.   Herbal medicines or extracts thereof consisting of oat stalks or leaves, carrots, licorice, hens, pearl barley, peony, hawthorn and chimpi, or extracts thereof, stalks or leaves of ogi 2.0, carrots 1.0, licorice 1.0, A type III collagen production promoter characterized by containing Hens 0.6, pearl barley 0.4, bakuga 0.4, sandalwood 0.4, hawthorn 0.2, and chimney 0.1.

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