KR100611796B1 - Cosmetic composition containing medicinal herbs called sipgeondaebodan and method for preparing the same - Google Patents

Abstract

The present invention provides a cosmetic composition containing ginseng, Baekchul, Bokryeong, Angelica, Cheongung, Sukjihwang, Peony, Astragalus, Broiler, and Licorice, and a manufacturing method thereof. Cosmetic composition according to the invention has a skin collagen synthesis, antioxidant and moisturizing effect.

Beyond the Ten Thousand (Gang), Ginseng, Baekchul, Bokryeong, Dongguk, Cheongung, Sukjihwang, Peony, Astragalus, Broiler, Licorice, Cosmetics

Description

Cosmetic composition containing ingredients rather than tens of chromosome and its manufacturing method {COSMETIC COMPOSITION CONTAINING MEDICINAL HERBS CALLED SIPGEONDAEBODAN AND METHOD FOR PREPARING THE SAME}

The present invention relates to a cosmetic composition containing a component rather than a tenth generation and a method for producing the same.

In general, the tenth Jeonbodan (Tang) is a remedy among the first remedies in Volume 5 of the "Pacific Peace and Mindification Defense" book, written in the Song Dynasty. After a big illness, the systemic weakness and severe blood loss, yin and yang, the front and the back, both inside and outside, Sagunja-tang, which is said to encourage men's spirit, and Samul-tang, which is said to strengthen the blood of women, It is a remedy for a comprehensive nutrition that is completed with 10 kinds of medicinal properties, and is known as a good medicine regardless of constitution and age and sex. It is currently used as an oral medication.

The efficacy and efficacy of each component rather than the above-mentioned ten generations will be briefly described below.

The jeonseondae than by activating the function of the central nervous system to enhance the non-specific immunity by promoting metabolism, stimulating the bone marrow to strengthen the erythropoiesis, improve blood vessels (血虛), promote blood circulation It is known to nourish and relieve colds and improve weakness.

Ginseng (人蔘) is one of the components of the jeonseondae, saponin accelerates the excitatory conduction of nerves and strengthens the conditional reflexes to reduce fatigue and increase work efficiency, Ginsenosides (ginsenosides) Rg1, Re, Rb2 Promotes the production of immune memory cells by enhancing the steroid hormone secretion of the adrenal cortex, adenosine, ginsenosides Rb1 and Re decrease the production of triglycerides in the blood, and ginsenosides Rg3 and Rg2 stimulate platelet clotting Inhibition is known to prevent thrombus formation. Pharmacological action is known to have the effect of hypoglycemic action, anticancer effect, serum protein synthesis promotion, antibody production promotion action, central excitement action, anti-fatigue action.

Baekchul is a β-odesmol (eudesmol) and hinesol (Hinesol) to suppress the central nervous system stimulation sedation, promotes digestive juice secretion ability to promote digestion, absorption and promote the release of stagnant venom in the gastric lumen It is known that the pharmacological action is contained vitamin A, D to nourish, converging pores to act as a limiting action.

In addition, the polysaccharide of pachyman, Pachyman, is a glucan with β-glucose as a main component and 1 → 3 binding. It supplies nutrition to all cells and activates brain cells. It stabilizes the mind and improves weakness, and the pharmacological action is known to have a gastric ulcer prevention effect, blood sugar strengthening and contact dermatitis inhibition.

Angelica has vitamin B12, folic acid, and iron to improve hematopoiesis by improving hematopoiesis, increase blood hematocrit level, and vitamin E, which acts on vitamin E deficiency and improves uterine development. Its pharmacological action is known to improve peripheral circulation disorder, sedative and analgesic action, and anti-inflammatory action of β-sitosterol-O-gluside.

In the uterus, the ferulic acid (ferulic acid) is analgesic, pain relief and smooth muscle relaxation effect to suppress the spasm of the intestines, contraction of the pregnant uterus, convulsions.

Jihwang (地 黃) belonging to the Hyunsam family has the action to help the blood of the human body and urinary reproductive function to blacken and moisturize the hair, and heukjihwang is more hematopoietic, self-acting action than Guangjihwang.

Peony (芍藥) Paeoniflorin (paeoniflorin) suppresses the central nervous excitability, pain relief, jingyeong, sedation and increases the convergence ability of the eccrine gland (계) acts with the basin (止汗).

Astragalus contains abundant nutrients such as sugars, folic acid, choline, amino acids, etc., it is tonic and supplies nutrients to the cells to suppress the excretion of the interstitial fluid to reduce the cold. In addition, by dilatating the blood vessels to reduce the stimulation of the Ecrine (Eccrine) gland to suppress excessive extracorporeal discharge of body fluids and strengthen the kidney function to promote diuresis.

In broilers, cinnamic aldehydes expand blood vessels to promote blood circulation, promote peripheral blood flow, and have strong dissipation and astringent effects on cold abscesses and ulcers at the skin end. .

Lastly, licorice (甘草) glycyrrhizin (glycyrrhizin) has an anti-allergic effect, ulcerative recovery and gastric spasms, and inhibits excessive gastric acid secretion.

However, plant components used as food or herbal ingredients may have different effects if they are applied differently, such as when administered orally, such as food or herbal medicines, and when applied to the skin, which itself is toxic. There are many things that can cause problems such as skin problems when applied directly to the skin of the human body. Therefore, even if it is used in the human body as a food or herbal ingredients, when applying them to the skin, it is necessary to adjust the type and dosage of plant components so as not to cause skin problems. In the case of the sub-Cheondaedae traditionally used as a herbal ingredient in herbal medicine has been proved to be safe for oral administration, and is known as a herbal ingredient with low intracellular toxicity, but it is not yet used in cosmetic compositions.

The present inventors do not administer to the human body by oral administration as in the conventional use, and when applied directly to the skin, it shows the effects of collagen biosynthesis, antioxidant, moisturizing and soothes the skin without causing problems to the skin. It has been found to improve skin texture.

Accordingly, an object of the present invention is to provide a cosmetic composition and a method for producing the same containing a component rather than a crossbone.

The present invention provides a cosmetic composition containing ginseng, Baekchul, Bokryeong, Dongguk, Cheongung, Sukjihwang, Peony, Astragalus, Broiler and Licorice.

In addition, the present invention

i) mixing ginseng, baekchul, bokyeong, donkey, cheongung, sukjihwang, peony, astragalus, broiler and licorice with a polyhydric alcohol solution, aging and filtering to obtain the filtrate, and

ii) preparing a cosmetic composition containing the filtrate

It provides a method for producing a cosmetic composition comprising a.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention, when applied to the skin of the Chinese herbal medicine ingredients known as jeonjeondaedan (tang), namely ginseng, baekrye, bokyeong, Angelica, Cheongung, Sukjiwang, Peony, Astragalus, broiler and licorice, collagen biosynthesis, antioxidant, moisturizing and It has been found that the effect of improving skin texture can be obtained, and it is therefore possible to invent a cosmetic composition containing these components.

In the present invention, the components may be added to the cosmetic composition in the form of a filtrate obtained by mixing them with an aqueous polyhydric alcohol solution, aging and filtering. Such extraction method is different from the conventional method of immersing in water, which is an extraction method of herbal medicine for oral administration.

In the above extraction method, it is preferable that the addition amount of the components rather than the cross-substance and the polyhydric alcohol solution is 10-50% by weight and 50-90% by weight, respectively. And it is preferable that the aqueous polyhydric alcohol solution which can be used by this invention is 20-50% of polyhydric alcohol. The polyhydric alcohol may include butylene glycol, propylene glycol, and the like, but is not limited thereto.

In addition, as a method of aging the mixture of the above components and a polyhydric alcohol solution, there is a method of using a reflux device in a water bath of 25-100 ℃, but not limited to this method if it can achieve the same effect Any method known in the art may be selected and used.

In addition, in the method of obtaining the filtrate, after filtration, it may be further carried out a decolorization and deodorization process.

In the present invention, the content of each component of the cross-subsequence can be different depending on the desired effect, such as skin improvement, skin protection. In one embodiment according to the present invention, 1 to 5% by weight of ginseng, 1 to 5% by weight of white ginseng, 1 to 5 based on the dry weight of each component Weight%, Angelica 1-5%, Cheongung 1-5%, Sukjihwang 1-5%, Peony 1-5%, Astragalus 1 Extracts of the above ingredients can be prepared by adding -5% by weight, 1-5% by weight broiler, and 1-5% by weight licorice to 50-90% by weight aqueous polyhydric alcohol.

It is preferable that the filtrate of the components rather than the cross-linked components prepared by the method described above is contained in an amount of 0.1 to 15% by weight based on the total weight of the cosmetic composition. If the filtrate is less than 0.1% by weight, the effect of antioxidant, etc. is weak, and if the filtrate exceeds 15% by weight may cause irritation to the skin.

The cosmetic composition according to the present invention is not limited to the formulation, and may take a formulation known in the art. For example, the cosmetic composition according to the present invention may have one formulation selected from a flexible lotion, a nutrient lotion, a nutrient emulsion, an emulsion cream (nutrition cream), a gel cream, a massage cream, an essence body oil, a makeup base, a foundation, and a pack. Can be.

Hereinafter, the present invention will be described in more detail with reference to Preparation Examples, Experimental Examples, and Prescription Examples, but these are intended to illustrate the present invention, and the scope of the present invention is not limited thereto.

Preparation Example 1: Preparation of extract 1 of the component

Ginseng 200g, Astragalus 200g, Bokryeong 200g, Baekchul 200g, Angelica 200g, Baekjak 200g, Cheonung 200g, Broiler 200g, 200g of sucrose and 200g licorice in a 45 ° C sealed container, to which methylparaben and phenoxyethanol were added as a preservative. This was then mixed with 5 kg of 30% butylene glycol aqueous solution. After aging for 7 days, the mixture was filtered and filtered. Activated carbon was added to the filtrate, followed by stirring for 6 hours, followed by filtration twice. The mixture was precipitated in a constant temperature bath at 4 ° C. for 1 day and filtered to obtain an extract 1.

Preparation Example 2 Preparation of Extract 2 of the Component

200 g of ginseng, 200 g of Astragalus, 200 g of Fuling, 200 g of Baekchul, 200 g of Angelica ginseng, 200 g of Baekjak, 200 g of Cheongung, 200 g of broiler, 200 g of sucrose and 200 g of licorice were mixed with 5 kg of 30% butylene glycol aqueous solution treated with an appropriate amount of preservative. After aging for 24 hours at 100 ° C., the mixture was filtered. Activated carbon was added to the filtrate, followed by stirring for 6 hours, followed by filtration twice. The mixture was precipitated in a 4 ° C. thermostat for 1 day and filtered to obtain Extract 2.

Collagen synthesis and antioxidant effects were measured in the following examples using extracts prepared in Preparation Examples 1 and 2. Red ginseng extract or green tea extract was used as a comparative substance.

Experimental Example 1

Determination of collagen synthesis effect

Experiment principle

A relatively specific, pigmented collagen by combining the basic group of the collagen molecule with the sulfone group of the serious red using picrosirius dye (trade name: Sirius red F3B, BDH, UK), which is a strong anionic acid dye. As a simple method, it has been used a lot recently. It has been used mainly for biopsy of tissues and for coloring collagen such as fibers, but it is a method applied to measuring the amount of collagen produced through cell culture.

Experiment method

Human normal fibroblasts were seeded in 96 well plates at 1 × 10 ⁵ / cell and dispensed with DMEM · F′10 (1: 1) medium containing 5% FBS. After 24 hours of incubation, the cells were replaced with fresh 5% FBS-containing DMEM.F'10 (1: 1) medium, and then the extract of the subsidiary group was treated to the concentration of Table 1 below. After incubation for 72 hours, the well plate was thawed while alternating with -20 ° C and room temperature to grind the cells. After the medium was well mixed, 1 ml of 0.1% serious red solution (solution dissolved in 0.1 g of serious red F3B in 100 ml of saturated picric acid) was added to 150 µl of the medium, and the mixture was left at room temperature for 24 hours.

After the reaction was centrifuged at 10,000 rpm for 5 minutes, the unbound serious red solution was discarded, washed three times with 0.01 N HCl, and 1 ml of 0.1 N NaOH was added to dissolve the collagen bound with serious red. This was measured for absorbance at 540 nm using an ELISA reader. All test results were corrected for absorbance in wells in which the cells were not cultured.

Collagen synthesis rate (%) of extract Concentration (w / w%) Sample One 0.5 0.25 0.1 0.05 0.025 0.01 0.001 Ingredient extract than ten generations 1 98 133 130 123 117 110 104 100 Rather than the tenth ingredient extract 2 98 135 128 125 120 116 102 105 Red Ginseng Extract (Control) - - - 152 142 133 127 119

From Table 1, it can be seen that the extract 1 extracted at 45 ° C for 7 days and the extract 2 extracted at 95 ° C for 24 hours when used at a concentration of 0.5%, the collagen synthesis effect of about 30% or more.

Experimental Example 2

Anti-oxidation effect measurement

DPPH (1,1-diphenyl-2-picryl-hydrazyl) method

DPPH was used as Sigma (SIG1, 1190, 1958) MA). DPPH was dissolved in methanol. At this time, since methanol may be evaporated if it is a stock solution, a stock solution was prepared and dissolved in a ratio of 6: 4 with distilled water. The dissolved DPPH solution was adjusted to have an absorbance of about 0.6 at 513 nm. 2 ml of DPPH solution was added to and mixed with 1 ml of the diluted extract sample. After standing at room temperature for about 1 hour, the absorbance at 513 nm was measured.

Anti-hydroxyl activity (%)            Concentration (w / w%) Sample 5 One 0.1 Ingredient extract than ten generations 1 99.4 38.8 12.2 Rather than the tenth ingredient extract 2 99.8 40.3 16.5 Green tea extract 99.6 94.5 68.7

From the above Table 2, it can be seen that when using more than 5% of the jeonseondae, extracts of jeonseondae than Preparation Example 1 and 2 shows an antioxidant effect similar to the green tea extract (Green Tea, scientific name: camellia sinensis).

NBT assay

Substrate / reaction solution was prepared by adding 1.6 ml of Triton X-100, 2.9 mg of EDTA, 1 mg of NBT (nitroblue-tetrazolium, Sigma), and 5.4 mg of hypoxanthine in 300 mM phosphate buffer (pH 7.8). 12 μl of xanthine oxidase was dissolved in 10 ml of 300 mM phosphate buffer (pH 7.8) to make an enzyme solution. 1.0 ml of the buffer was placed in the spectrophotometer cell, and 0.5 ml of the prepared substrate / reaction solution was added. 100 μl of the samples in Table 3 diluted according to the concentration was treated. 0.5 ml of enzyme solution is added thereto and reacted at 37 ° C. for 30 minutes, and then absorbance is measured at 540 ml.

Anti-Superoxide Anion Activity (%)            Concentration (w / w%) Sample 5 One 0.1 Ingredient extract than ten generations 1 92.4 36.9 10.6 Rather than the tenth ingredient extract 2 91.8 40.5 18.2 Green tea extract 91.3 68.5 43.6

Through the above Table 3, it can be seen that the extracts 1 and 2 than the sub-Taekdae both show an anti-superoxide anion activity effect similar to the green tea extract at a concentration of 5%.

DCFH-DA (using mouse fibroblast L929)

Transformed mouse fibroblast L929 recovered by Trypsinization was suspended in DMEM.F'10 medium containing 5% BCS, and then cell suspension (5000-10000cells) in each well of a 96 well tissue culture plate. / well) 100 μl was inoculated and then incubated at 37 ° C. under 5% CO ₂ conditions for 24 hours. Subsequently, each well was treated with 10 μl of the sample shown in Table 4, followed by further incubation for 15 minutes, 30 minutes, 45 minutes, 60 minutes and 120 minutes, followed by 100 μl of neutral red solution ( neutral red solution) (50 µg / ml) was added and reacted for 3 hours.

Neutral Red is completely concentrated through the plasma membrane into the lysosomes of live or intact cells, fixed with 100 μl of 1.0% formalin / 1.0% CaCl ₂ and then using 1.0% acetic acid / 50% ethanol solution. Neutral red in the cells was extracted. The extracted neutral red was measured for absorbance at 540 nm using an ELISA reader. All experimental results were corrected with absorbance measured in wells without cultured cells.

Antioxidant Stress Activity (%)             Sample type and concentration 15 30 45 60 Ingredient extract than ten generations 1 One% 35.1 37.5 49.6 54.4 0.5% 25.4 28.9 31.6 40.0 Rather than the tenth ingredient extract 2 One% 63.6 60.5 62.1 69.1 0.5% 52.4 59.6 57.3 56.2 1% Green Tea Extract 62.1 64.5 65.4 63.2

Through Table 4, it can be seen that the extract 2 extracted at 95 ℃ for 24 hours shows a similar effect to the green tea extract in the intracellular antioxidant stress activity.

Hereinafter will be described a prescription example according to the formulation of the cosmetic composition according to the present invention.

[Prescription Example 1]

Flexible skin lotion (skin) containing a component rather than a crossbone according to the present invention can be prescribed in the following configuration.

ingredient Content (% by weight) Extract 1,3-butylene glycol glycerin oleyl alcohol polysorbate 20 ethanol preservative flavor water 10.00 2.00 3.00 2.00 1.00 6.00 Proper Proper Proper system 100.00

[Prescription 2]

Prescription examples of nutrient latex (milk lotion) containing a component rather than the thyroid in accordance with the present invention is as follows.

ingredient Content (% by weight) Extract 1,3-butylene glycol glycerin oleyl alcohol polysorbate 20 preservative flavor water 10.00 4.00 3.00 0.50 1.00 appropriate quantity appropriate quantity system 100.00

[Prescription 3]

A prescription example of a nourishing cream containing a component rather than a tenth generation according to the present invention is as follows.

ingredient Content (% by weight) Rather than the subsidiary component extract 1,3-butylene glycol glycerin triethanolamine tocopheryl acetate liquid paraffin squalane macadamia nut oil polysorbate 60 sorbitan sesquioleate carbomer preservative flavored water 10.00 2.00 4.00 0.50 1.00 5.00 5.00 15.00 1.00 1.00 0.30 Proper Proper Proper system 100.00

[Prescription 4]

A prescription example of a massage cream containing a component rather than a crossbone according to the present invention is as follows.

ingredient Content (% by weight) Ingredients of Vaseline Extract Vaseline Liquid Paraffin Beeswax Polysorbate 60 Sorbanthesquioleate Propylene Glycol 10.00 5.00 30.00 3.00 1.00 1.00 5.00 5.00 Proper Proper Proper system 100.00

[Prescription 5]

Prescription example of a pack containing a component rather than a cross according to the present invention is as follows.

ingredient Content (% by weight) Component extract 1,3-butylene glycol polyvinyl alcohol polyvinylpyrrolidone ethanol polyvinylacetate preservative flavor water 10.00 5.00 12.00 2.00 8.00 1.00 Proper Proper Proper system 100.00

[Prescription 6]

A prescription example of an essence containing a component rather than a cross teen according to the present invention is as follows.

ingredient Content (% by weight) Extract 1,3-butylene glycol glycerin triethanolamine carboxyvinyl polymer xanthan gum preservative flavor water 10.00 2.00 5.00 0.27 0.20 0.10 Trace Trace Amount system 100.00

The present invention provides a skin collagen synthesis, antioxidant and moisturizing effect by adding to the cosmetic composition for applying the skin to the cosmetic composition for skin application, which is used only by oral administration such as food or Chinese medicine as a medicine for health to the body, age, It can contribute to the prevention of skin aging, skin protection, and skin improvement by enhancing the physiological activity of the skin degraded by stress, ultraviolet rays, environmental pollution, and drying.

Claims (6)

Ginseng, Baekchul, Bokryeong, Danggwi, Cheongung, Kyojihwang, Mature Jichu, Peony, Astragalus, Broiler and Licorice Contains 10-50% by weight of the filtrate extracted by 50-90% by weight of 20-50% aqueous solution of any one of butylene glycol and propylene glycol in an amount of 0.1-15% by weight based on the total weight of the cosmetic composition. Cosmetic composition. delete delete delete The formulation of claim 1, wherein the cosmetic composition is selected from the group consisting of a flexible lotion, a nourishing lotion, a nutrient emulsion, an emulsion cream (nutrition cream), a gel cream, a massage cream, an essence body oil, a makeup base, a foundation, and a pack. Having a cosmetic composition.  i) 10-50% by weight of ginseng, baekchul, bokyeong, angwi, cheongung, sukji sulfur, peony, astragalus, broiler and licorice 50-90% by weight of any one of 20-50% aqueous solution of butylene glycol and propylene glycol Mixed with, aged and filtered to obtain the filtrate, and ii) preparing a cosmetic composition containing the filtrate 0.1 to 15% by weight based on the total weight of the cosmetic composition Method for producing a cosmetic composition comprising a.