KR20180045187A - Cosmetic composition for improving moisturizing and antioxidant containing ultrasonicating extract of cucumber seed - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an ultrasonic extract of cucumber seeds, and more particularly, to a cosmetic composition containing a cucumber seed extract having enhanced moisturizing and antioxidation effects by ultrasonically extracting cucumber seeds. According to the present invention, the cosmetic composition has excellent antioxidative effect, skin moisturizing effect, and skin barrier improving effect, and has excellent stability that does not cause discoloration, separation and precipitation in response to a temperature change.

Description

Technical Field [0001] The present invention relates to a cosmetic composition containing an extract of Oocyese Ultrasonics and an antioxidant cosmetic composition,

The present invention relates to a cosmetic composition having skin moisturizing and antioxidant effects, and more particularly, to a cosmetic composition having an excellent moisturizing effect and antioxidative activity by containing an Oocyte ultrasound extract as an active ingredient.

The goal of cosmetics is to maintain beautiful and healthy skin, and also to delay the aging of naturally occurring skin as it gets older. In general, aging is caused by oxidative damage of biomaterials by free radicals. That is, reactive radicals such as active oxygen such as hydrogen peroxide, hydroxy radical, and superoxide radical peroxidize the lipid constituting the cell membrane to destroy the cell membrane, oxidize the protein, attack the nucleic acid and cause oxidative damage, Destroys and eventually kills cells. Skin characteristics that are most noticeable due to aging include wrinkles, decreased elasticity, and skin dullness.

In addition to wrinkles and elasticity decline, after 40s, facial color is not clear and is often gray and dark. Especially for young women, when they experience childbirth, they have eosinophilia, blood circulation disorder, which causes their metabolic ability to fall and their skin to become rough and rough. When the metabolism ability is lowered and the skin becomes rough, the water content is also easily reduced. Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer to about 10 to 30% in the keratin layer. The keratinocyte layer is known to have an important role in the moisturization of the skin due to the existence of a substance having a hydrophilic water retention capacity called a natural moisturizing factor (NMF). Normal keratinocyte layer maintains skin smoothness and softness and maintains normal body protection function when moisture is maintained at about 10 to 30%.

Therefore, proper maintenance of the moisture content of the keratinocyte layer is very important for preventing skin aging. However, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, There is a problem that the emulsion stability of the cosmetic is impaired due to the strong electrolyte. Amino acids, collagen, elastin, etc. are also capable of moisturizing, but also had a limited ability to moisturize. The ability of the horny layer to retain moisture can be controlled by natural moisturizing factor (NMF), which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. And development of a substance having excellent moisturizing effect is one of important research subjects in cosmetics. Materials known to have antioxidant activity include ascorbic acid, dl-a-tocopherol, beta-carotene, dibutylhydroxyltoluene (BHT) and the like However, since these materials are used as a synthetic product, there is an urgent need to develop new antioxidative cosmetics that can be applied to skin safely and usefully, because they may cause problems of skin safety when used for a long time or in a large amount.

Therefore, studies on skin aging and moisturizing improvement are expected to be continuously carried out. Many cosmetic products using natural products are being developed in order to solve such skin problems and to reduce skin irritation caused by various chemicals. Natural materials are not only less harmful to the skin, but also have increased their value as a raw material for cosmetics due to the recent popularity of cosmetics using natural materials.

For this purpose, researches are being actively carried out to develop materials having excellent safety and skin beauty effect by searching skin physiological activity of various natural vegetable persons.

Cucumber is an annual plant of the dicotyledonous plant of the dicotyledonous plant. It has coarse hairs along with ridgelines. It grows long by attaching to other objects while wrapping it with tendrils. Leaves are alternate phyllotaxis, long petiole, shallowly split with palm, serrated on edge, and rough. Flowers are unicellular, bloom from May to June in yellow, with a diameter of about 3cm and wrinkles.

The corolla is divided into five, the male flower has three stamens, and the male flower has a long ovary with a thorn like projection on the lower side. The fruit is a perennial type with berries, and when it is young, it has thorn like protrusions. It ripens from green to dark yellowish brown. The seeds are yellowish white. Cucumber is one of the most important edible crops, and the juice also helps to cool it when it is put in hot water.

Cucumbers are fruitful for about 40 ~ 50 days to obtain mature seeds. The best temperature for cucumber seeds is 25 ~ 30 ℃ and the temperature suitable for growing is 15 ~ 29 ℃. When the temperature is above 30 ℃ and below 10 ~ 12 ℃, it is affected by growth disorder. When the fruit is large, it requires a high temperature of 20 ~ 30 ℃.

In relation to cucumber, Korean Patent Laid-Open Publication No. 2003-0077872 discloses a whitening cosmetic composition comprising phytolite derived from an extract selected from the group consisting of cucumber extract, apple extract, golden root extract, camellia extract and mixtures thereof as an active ingredient . Korean Patent Laid-Open Publication No. 2015-0062577 discloses various methods for extracting major components of cucumber.

Therefore, the inventors of the present invention discovered that the skin moisturizing and antioxidative effects are greatly improved by a specific extraction method when a cucumber extract is prepared using ultrasonic extraction instead of the conventional methods such as hot water extraction, room temperature extraction, Thereby completing the invention. It is an object of the present invention to provide a cosmetic composition which contains an extract of Cucumber extract as an active ingredient to provide an antioxidative effect, skin moisturizing effect and skin barrier improving effect.

The above object is achieved by a cosmetic composition containing an Oocyte ultrasound extract and having an antioxidative effect, a skin moisturizing effect or a skin barrier improving effect.

Preferably, the oocyte supernatant extract is selected from the group consisting of purified water, anhydrous or lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether , Butylene glycol, hexane, and mixtures thereof, and then extracted with an ultrasonic extruder for about 1 to 12 hours.

Also, preferably, the Oocyte ultrasound extract may be added in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Also preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Or a pharmaceutically acceptable salt thereof.

The present invention can provide a cosmetic composition containing an OIC ultrasound extract, which is applicable to skin cosmetics and is harmless to the human body and has excellent safety, as an active ingredient. The present invention provides a cosmetic composition having an effect of improving the antioxidation, skin moisturization and skin barrier. The cosmetic composition containing the ultrasound extract of the present invention can be used as a cosmetic composition containing a conventional cucumber extract Is superior to the skin moisturizing effect.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In addition, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The term "about" is used herein to refer to a reference quantity, a level, a value, a number, a frequency, a percentage, a dimension, a size, a quantity, a weight or a length of 30, 25, 20, 25, 10, 9, 8, 7, Level, value, number, frequency, percentage, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

Throughout this specification, the words "comprises" and "comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

The inventors of the present invention have investigated the possibility of application as a cosmetic composition containing Oicosone supernatant extract from a variety of natural products, and found that antioxidation, moisturizing and skin barrier improvement effects can be measured through various experiments, and as a result, the efficacy as a cosmetic product can be expected. An extract having such excellent efficacy was obtained in a maximally high yield to develop a cosmetic composition showing more excellent effects.

The cosmetic composition of the present invention as described above is a cosmetic composition containing an Oocyte ultrasound extract by sonication of oocyte. The cosmetic composition of the present invention comprising the above components is suitable as a cosmetic composition for antioxidation, moisturizing and skin barrier improvement of skin.

In order to obtain maximally high yield of cucumber having various effects, the present invention treats cucumber with ultrasonic extraction method. By this treatment, it has been confirmed that the content of active ingredient is increased, and it is applied to cosmetics for antioxidation, The present invention has been completed.

The "ultrasonic extraction" of the present invention is characterized in that after washing and pulverizing the cucumber, water, anhydrous or low-boiling alcohol having 1 to 4 carbon atoms, acetone, glycerin, ethyl acetate, butyl acetate and 1,3-butylene glycol, And the mixture is placed in at least one extraction solvent selected from the group consisting of dichloromethane, chloroform, ethyl ether, butylene glycol, hexane and mixtures thereof, and the mixture is stirred at a temperature of 20 to 40 ° C (room temperature) Means extraction with the extractor for about 1 to 12 hours. The time of the ultrasonic extraction may be varied depending on the amount of the sample to be extracted, and the result of the extraction may be filtered or purified.

In addition, the OIC ultrasound extract of the present invention may be prepared into a powder state by an additional process such as vacuum distillation, freeze-drying, or spray drying.

According to a preferred embodiment of the present invention, the content of the Oocyte ultrasound extract added to the cosmetic composition is 0.0001 to 30.0% by weight, preferably 0.001 to 25.0% by weight, and most preferably 0.1 to 10.0% Weight%. When the content of the OIC ultrasound extract is less than 0.0001% by weight, the effect of improving the antioxidation, moisturizing and skin barrier is not exhibited. When the content is more than 30% by weight, the effect of increasing the content is insignificant, And it is not economical.

The ingredients contained in the cosmetic composition of the present invention include, as an active ingredient, the ingredients commonly used in cosmetic compositions in addition to the above-mentioned effective ingredients, such as conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, .

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Production Examples. These production examples are only for explaining the present invention in more detail and that the scope of the present invention is not limited by these production examples according to the gist of the present invention, It will be obvious.

< Manufacturing example  1> Manufacture of Cucumber Solvent Extract

The oocyte was washed with purified water, dried, and then crushed and made fine using a sieve of 100 mesh. 70% ethanol was added thereto so that the amount of the crushed cucumber was 100 g / L, and the mixture was refluxed three times for 5 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper and concentrated under reduced pressure and freeze-dried at 50 ° C or lower. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a solvent extract of Cucumber (Preparation Example 1).

< Manufacturing example  2> Manufacture of Oishi Warm Extract

The oocyte was selected as a sample from which the oocyte was heated and sufficiently washed, and the crushed product was made finer so as to pass through the 300 mesh sieve. 70% ethanol was added thereto so as to have a concentration of 100 g / L, and the mixture was refluxed at 95 DEG C for 4 hours. After cooling, the mixture was filtered through Whatman # 3 filter paper and concentrated under reduced pressure at 50 DEG C or below. Respectively. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a cucumber warm-up extract (Preparation Example 2).

< Manufacturing example  3> Preparation of cucumber ultrasound extract

The cucumber was prepared as an extract using an ultrasonic extractor. The cucumber was selected as a sample, thoroughly washed, and finely pulverized so as to pass through a 100 mesh sieve. 70% ethanol was added to the cucumber pulp to a concentration of 100 g / L, and the mixture was extracted with an ultrasonic wave extractor (natural solution) at room temperature for 4 hours. After cooling, the mixture was filtered through a filter of whatman # 3, Concentrated and lyophilized. The lyophilized product was dissolved in 50% glycerin to 2% (w / v) to prepare a cucumber ultrasound extract (Preparation Example 3).

< Manufacturing example  4> Manufacture of OIC Ultrasound Extract

The oocyte was extracted with an ultrasonic extractor. The oocyte was selected as a sample, washed thoroughly, and finely pulverized to pass through a 300-mesh sieve. 70% ethanol was added thereto so that the amount of the crushed cucumber was 100 g / L, and the mixture was extracted with an ultrasonic wave extractor (natural solution) at room temperature for 4 hours. After cooling, the mixture was filtered through a Whatman # 3 filter paper, Concentrated and lyophilized. The lyophilized product was dissolved in 50% glycerin to a concentration of 2% (W / V) to prepare a yeast ultrasound extract (Preparation Example 4).

< Formulation Example  1>

Formulation examples of creams in cosmetics containing Oi-C ultrasound extract (Preparation Example 4) are shown in Table 1 below.

ingredient Content (unit:% by weight) Oocyese Ultrasound Extract (Preparation Example 4) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Formulation Example  1>

Comparative Formulation Example 1 of the cream not containing the Oocyte Ultrasonic Extract is shown in Table 2 below.

ingredient Content (unit:% by weight) glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Formulation Example  2>

Formulation examples of creams in cosmetics containing a Cucumber herbal extract (Preparation Example 1) are shown in Table 3 for Comparative Prescription Example 2.

ingredient Content (unit:% by weight) Cucumber Solvent Extract (Preparation Example 1) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Formulation Example  3>

Comparative Formulation Example 3 of cream containing oicinaceous warming extract (Preparation Example 2) is shown in Table 4 below.

ingredient Content (unit:% by weight) The warming extract of Oishi (Preparation Example 2) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

<Comparison Formulation Example  4>

Comparative Formulation Example 4 of a cream containing cucumber ultrasound extract (Preparation Example 3) is shown in Table 5 below.

ingredient Content (unit:% by weight) Cucumber Ultrasonic Extract (Preparation Example 3) 5.0 glycerin 5.0 Sodium hyaruronate 0.1 Polysorbate 60 1.5 Mineral oil 5.0 Squalane 3.0 Dimethicone 2.0 Triethanolamine 1.0 Chin type monostearin glycerin 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Sorbitan stearate 0.6 Sodium magnesium silicate 0.1 Betaine 3.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Balance system 100

< Experimental Example  1> Antioxidant effect measurement

The antioxidative activity of the extracts prepared in Production Examples 1 to 4 of the present invention was measured by the Tiobarbituric acid method (TBA assay) using a lipid peroxidation system by Fenton reaction. That is, in 5 ml of 25 mM Trizma hydrochloric acid / 0.75 mM potassium chloride buffer containing 10 μl of ethyl linoleate, 0.2% sodium dodecylsulfoxide (SDS), 10 μmol of ferrous chloride and 2 μmol of hydrogen peroxide, The dimethylsulfoxide (DMSO) test solution of one extract was added and incubated at 55 ° C for 16 hours. Then, 50 μl of 4% dibutylhydroxyltoluene (BHT) was added to stop the reaction. 0.3 ml of this solution was put into a test tube, and 3.0 ml of 0.05 N hydrochloric acid and 0.67% thiobarbituric acid (TBA) solution were added and boiled for 30 minutes. After cooling the reaction solution, 4.0 ml of 85% butanol was added, and the absorbance of the butanol layer after centrifugation was measured at 535 nm. The antioxidative effect of each extract was calculated by the following equation (1).

The results are shown in Table 6 below.

Test substance Antioxidative effect(%) Experimental concentration (10 / / ml) Experimental concentration (50 / / ml) Experimental concentration (100 / / ml) Production Example 1 21 45 60 Production Example 2 28 50 62 Production Example 3 31 51 70 Production Example 4 30 55 76

The antioxidative effects of the extracts of the extracts of Preparation Examples 1 to 4 were mostly high, and the extracts of the extracts of Preparation Example 4 according to the present invention showed excellent antioxidative effects.

Antioxidant activity of cucumber extracts of Preparation Examples 1 to 4 and ascorbic acid, dl-alpha-tocopherol, beta-carotene and dibutylhydroxytoluene (BHT), which are antioxidants, Activity was measured by the same method as Experimental Example 2 to compare the IC ₅₀ of 50% inhibitory concentration.

The results are shown in Table 7 below

Test substance IC ₅₀ (10 μg / ml) Production Example 1 50.5 Production Example 2 47.4 Production Example 3 45.0 Production Example 4 41.2 Ascorbic acid 21.90 Dl-alpha-tocopherol &lt; / RTI &gt; 3.36 Beta-carotene 29.8 Dibutylhydroxyltoluene (BHT) <1

The cucumber extracts of Preparation Examples 1 to 4 showed high activity comparable to those of other antioxidants, and in particular, the extract of Cucumber supernatant of Preparation Example 4 showed high activity. Especially, these cucumber extracts have remarkable antioxidant activity despite being a mixture of natural products.

&Lt; Experimental Example 2 > Evaluation of skin moisturizing effect of Osiecanic ultrasound extract

The wrinkle-improving effect of the cream of Oishi's ultrasound extract (Formulation Example 1) and the cream of Comparative Formulation Example 1 to Comparative Formulation Example 4 was evaluated as follows.

Twenty-five to twenty-four patients without skin disease were divided into two groups each consisting of 20 patients per group. The nutritional creams of Formulation Example 1 and Comparative Formulation Examples 1 to 4 were administered twice daily Month and then applied to the face and forehead. The skin conductivity was measured by using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) under the constant temperature and humidity conditions (24 ° C, 40% humidity) before the start of application, and the skin conductivity was measured at 1 week, 2 weeks, The rate of increase in skin conductivity (%) was measured and the rate of increase was evaluated. The results are shown in Table 8 below.

Experimental product After 1 week after 2 weeks After 3 weeks Formulation Example 1 43 48 52 Comparative Formulation Example 1 17 20 24 Comparative Formulation Example 2 19 25 31 Comparative Formulation Example 3 20 26 32 Comparative Formulation Example 4 25 28 35

As shown in Table 8, in the case of the cream containing Formulation 1 of oocyte supernatant, the rate of increase of skin conductivity was much higher than that of Comparative Formulations 1 to 3. Since the skin conductivity is generally proportional to the skin moisture content, the above results confirm that the cosmetic composition containing the Oocyte ultrasound extract keeps the skin moisture content higher than the other cosmetic ingredients. Skin irritation could not be observed in most of the subjects who applied this cosmetic composition to the skin.

< Experimental Example  3> Evaluation of Skin Barrier Improvement Effect of Ultrasonic Extract of Oi

In order to examine the skin barrier improvement effect of the cosmetic composition containing the extract of the present invention of the present invention, the following experiment was conducted. In this Experimental Example, the cosmetic material of Formulation Example 1 was evaluated by performing a comparative experiment using a Tewameter (TM300, Courage and Khazaka Electronic Co., Germany) for transepidermal water loss (TEWL). Each sample was applied to the right and left faces of 40 women in their 20s and 40s. Percutaneous water loss before, 1, 2, 4, and 6 hours was applied before and 3 cm from the eye. The results are shown in Table 1. The results are shown in Table 1. The results are shown in Tables 1 and 2. The results are shown in FIG. The results are shown in Table 9 below.

division 1 hours 2 hours 4 hours 6 hours Prescription Example 1 15.3 14.9 12.1 8.9 Comparative Formulation Example 1 22.0 21.5 20 16 Comparative Formulation Example 2 18.9 17.3 15.2 13.1 Comparative Formulation Example 3 17.0 16.2 14.2 12.1 Comparative Prescription Example 4 16.2 15.8 15.2 14.8

As shown in Table 9, the amount of transdermal water loss was measured. As a result, the amount of transdermal water loss was reduced in the facial skin of an experimenter who applied cream containing Oi-echo ultrasound extract, I could.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

A cosmetic composition having an antioxidative effect comprising an Oocyte ultrasound extract. A cosmetic composition having a skin moisturizing effect comprising an Oocyte ultrasound extract. A cosmetic composition having an effect of improving the skin barrier comprising an Oocyte ultrasound extract. The method according to any one of claims 1 to 3, wherein the Oocyte supernatant extract is selected from the group consisting of purified water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3- Is added to an extraction solvent selected from the group consisting of propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, and mixtures thereof, followed by extraction with an ultrasonic extractor for about 1 to 12 hours. 4. The cosmetic composition according to any one of claims 1 to 3, wherein the Oocyte ultrasound extract is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. 4. The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing oil, powder foundation, emulsion A cosmetic composition, a foundation, a wax foundation, and a spray.