KR20110040024A - Mixture extract of natural plants and anti-atopy cosmetic composition containing the same - Google Patents

Abstract

PURPOSE: A cosmetic composition containing herb extract is provided to ensure antioxidation and to suppress lipoxiganase activity and to treat and prevent allergy and atopic dermatitis. CONSTITUTION: A cosmetic composition contains0.001-5 weight% of mixture extract of Coptis japonica, Phellodendron amurense root skin, Scutellaria baicalensis root, Polygonatum sibiricum root, and Astragalusoptis membranaceus. The mixture extract is isolated using water, alcohol of 1-4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, butylene glycol, or hydrate thereof. The cosmetic composition is manufactured in the form of skin softener, massage cream, nutrition serum, essence, or pack. The cosmetic composition is used for antioxidation or anti-inflammation.

Description

Mixture extract of natural plants and anti-atopy cosmetic composition containing the same {H5-S．ａｕｒｅｕｓ コ ＭＰＬＥＸ ™)

The present invention relates to a mixed extract of a natural plant and a cosmetic composition containing the same.

Atopic dermatitis occurs mainly in infancy and is also called fever, mainly due to a decrease in the amount of ceramides in the stratum corneum. As atopic dermatitis is deteriorated in the stratum corneum, the skin dries up, resulting in a large amount of dead skin, and as the skin barrier is deteriorated, irritant substances such as antigenic proteins, which are difficult to invade in normal skin, enter the stratum corneum. The back develops easily and shows severe pruritus. Therefore, more than 90% of atopic patients are infected with Staphylococcus aureus, which may be caused by scratching because the patient cannot tolerate itching, but recent reports suggest that the toxins of this bacterium stimulate the body's immune system and cause allergies. It is said to make atopic dermatitis worse.

The causes of such atopic skin are largely divided into four. One of the causes is a genetic cause, and gamma-linoleic acid, an essential fatty acid that forms intercellular lipids, which is a binding agent between the phospholipid and keratinocytes of the cell membrane, which is a skin protective film that is responsible for skin protection. ; GLA) is not enough to make the skin protective layer hard, so moisture on the surface of the skin is easily lost and allergens are easily generated by external stimulation. Other causes are environmental causes. As the living environment of modern people changes to apartments, allergens are sensitive to small irritations due to the decrease in skin moisture retention and loss of skin protection function as the living environment dries. Another cause is caused by free radicals, which are caused by free radicals to generate lipid peroxides and cause atopy. Another cause is infection caused by bacteria, viruses, fungi, etc. (Dermatology, Korean Dermatology Publishing Committee, Yeomungak, 1992).

Therefore, in order to treat such atopy, it is essential to develop a material having excellent antibacterial, moisturizing and anti-inflammatory effects, no side effects and low cost, and easy-to-use cosmetics containing the material.

On the other hand, Coptis japonica is a perennial herb of Ranunculuaceae, cultivated all over Japan. Root stems are called Huangyan (黄連), blue heat saf (청 火), cheongsimjebun (淸 心 除煩), humidity control, detoxification, insecticidal, epidemic fever, typhoid fever, obesity area (痺 滿 嘔 逆) ), Bacterial diarrhea, abdominal pain, pulmonary tuberculosis, vomiting, nasal bleeding, bleeding, small eyes, eye redness (目 充血), stomatitis is known to treat. The ingredient contains a lot of alkaloid compounds, and the main component of berberine is about 7 to 10%, in addition to coptisine, palmatine, obacunone, It is known to contain obaculactone [Bae Gi-hwan, Korean medicinal plant, Kyohaksa, 137 (2000)].

Astragalus optis membranaceus is a perennial plant of the legume (Leguminosae), which grows in the mountains of Gyeongbuk, Gangwon, Hamnam, and Hambuk, and is distributed in Manchuria, Mongolia, Japan, Siberia, and Central Asia. The roots are called Astragalus, and the fresh ones are ripe, and they have the benefits of dihydrogen species, and they treat Jahan, Dohan, and hemophilia. , Mild (蜜炙 中) has the effect of Bojungikgi (補中益氣). In addition, it is known to treat internal wounds, non-hearing diarrhea, and debilitating blood leaves, and the leaves have the effect of jigal, and to treat muscle spasms and carbuncles. have. Its main ingredient is flavonoid, which is known to contain formonetin, 3-hydroxyformonetin, etc. [Bae Gi-hwan, Korean medicinal plant, Kyohaksa, 242 (2000)].

Phellodendron amurense is a rutaceae, which grows in deep mountain forests in Korea except Jeju and Jeonnam, and is distributed in Japan, Manchuria, China, and Ussuri. Stem bark is called yellow-white (黄栢), has the effect of clearing, humidity, degenerative fever, metabolism, detoxification, hari, diabetes, jaundice, paraplegia, dreams due to sequence It is known to treat bloody stool and venom. Ingredients are known to contain alkaloids such as berberine, jatrorrhizine, magnoflorine, phellodendrine, candicine, palmatine, menisperine, etc. in the stem bark. 2000)].

Scutellaria baicalensis is a perennial herb of Labiatae, which grows in northern mountains and is distributed in Manchuria, China, Amur, Mongolia and East Siberia. Root is called golden (黄 芩), has the effect of hemostasis, anxiety, bungae (폐), lung fever, sea water, jaundice, uterine bleeding, long-term pain (目赤 腫痛), Taedong anxiety (胎動 不 安) Cure). Its ingredients are known to contain baicalin, baicalein, woogonin, etc. [Bae Gi-hwan, Korean medicinal plants, Kyohaksa, 446 (2000)].

Polygonatum sibiricum ) is a perennial plant of the Liliaceae family, growing in the mountains and fields of Hamgyeong-do, Pyongnam (Eulmildae), and distributed in Manchuria (Yongjeong, Yancheng, Yonghua). Root stem is called Hwangjeong (黃 精), Bojungikgi (윤 中 益氣), Yun Shim lung (潤 心肺), Kang Geun bone (强 筋骨) has the efficacy of, fever loss fever, lung fever, customs It is known to cure pain. The mucus of the rhizome is known to be falcatan, and it is also known to contain polygonaquinone [Bae Gi-hwan, Korean medicinal plant, Kyohaksa, 539 (2000)].

Currently used atopic skin treatments include 1) moisturizers, 2) taricides, 3) external steroids such as triamcinolone and fluocinolone, and oral steroids, 4) UV treatment, 5) clindamycin, dicloxacilline. Such as antibiotics, 6) antipruritic agents, 7) immune regulation, immunosuppression, immunotherapeutics, 8) food, fragrance, herbal medicines. However, tar formulations have the disadvantage of smelling bad skin and irritating dry skin due to alcohol content, and steroid preparations have serious side effects such as rash, skin color change hypertension, cataract, and UV treatment, causing sunburn, itching and pigmentation. In long-term treatment, there is a high risk of premature aging of the skin and cancer, and antibiotics are mainly used as oral medicines. In addition, immunomodulators and inhibitors are most commonly used in recent years, but the use of them should not be used on faces infected with viruses such as chickenpox and shingles. . In addition, food therapy, herbal treatments, and other fragrance therapy has no side effects on use, but the effect is often insignificant, so it is difficult to be a fundamental treatment, there may be a limit on its use.

Therefore, there is a need for a cosmetic composition that is excellent in atopic skin improvement effect, has low skin irritation, is low in production cost, and is easy to use.

The purpose of the present invention is excellent in anti-inflammatory, antioxidant, cell proliferation, degranulation inhibition, skin itching, atopic skin improvement, and no harmful chemicals are added, so it does not have any problems in stability and safety. And Astragalus mixed extract and a cosmetic composition containing the same.

The present invention is a mixture of extracts of sulfur, sulfur, yellow, yellow, yellow and sulfur based mixtures with one or more solvents selected from water, alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, butylene glycol, and their hydrates Provide an extract.

In the present invention, the yellow lotus, yellow white, golden, yellow and yellow group may be purchased from the Korean traditional market, or may be directly collected from Yasan or field.

In the present invention, the mixed dry weight ratio of the yellow lotus, the yellowish white, the golden, the yellow gold and the yellow group may be variously changed. For example, 1-5: 1-5: 1-10: 1-10, 1: 1: 1: 1: 1, 1: 2: 2: 1: 1, 2: 1: 1: 1: 1, 1: 2: 1: 1: 1, 1: 1: 2: 1: 1, 1: 1: 1: 2: 1, 2: 2: 1: 1: 1, 2: 1: 2: 1: 1, 4: 1.5: 1.5; 1.5: 1.5, 1.5: 4: 1.5: 1.5: 1.5, 1.5: 1.5: 4: 1.5: 1.5, 1.5: 1.5: 1.5: 4: 1.5, 1.5: 1.5: 1.5: 1.5: 4, It may have a dry weight ratio, such as 4: 1.5: 1.5: 1.5: 1.5 is preferred.

Extracts of the present invention are prepared by known extraction methods known to those skilled in the art. For example, in the present invention, the yellow rhubarb, yellow white, golden, yellow gold and yellow-yellow mixed extract is finely mixed with dried products of yellow lotus, yellow-white, golden, yellow-yellow and yellow, and 1 to 15 times the volume of water of the dry weight, After extracting an active ingredient by adding an extraction solvent selected from a lower alcohol having 1 to 4 carbon atoms or a mixed solvent of these lower alcohols and water, and dipping at 4 to 30 ° C. for 3 to 20 days, the extractant is concentrated by a vacuum concentrator. do. In addition, in the present invention, the yellow lotus, yellowish white, golden, yellow and yellow seed mixed extract is ground and mixed with the dried product of yellow lotus, yellowish white, golden, yellowish yellow and yellow, and 1 to 15 times the volume of water, carbon number 1 of the dry weight. At least one solvent selected from the group consisting of -4 lower alcohols or a mixed solvent of these lower alcohols with water, ethyl acetate, water, glycerin, ethylene glycol, propylene glycol, butylene glycol, and then the solvent is evaporated. In order to prevent extraction by heating with an extractor equipped with a cooling condenser for 30 to 100 ℃ for 3 to 48 hours or by immersion at 5 to 30 ℃ for 1 to 5 days to extract the active ingredient, the extraction solvent is obtained by concentrating with a vacuum condenser .

Moreover, it can manufacture using well-known ultrasonic extraction method.

The extract of the present invention is preferably extracted with sulfur, sulfur white, golden, sulfur and sulfur mixture with water, anhydrous or hydrous alcohol of 1 to 4 carbon atoms, the extraction temperature is preferably 50 to 100 ℃.

The present invention also provides a cosmetic composition containing the mixed extract of the present invention.

In the cosmetic composition of the present invention, the yellow, yellow, yellow, yellow, and yellowish mixed extracts may be added to the cosmetic in an amount of 0.1% to 10% by weight based on the total liquid weight of the cosmetic composition, and also the total dry weight of the cosmetic composition. It can be added to the cosmetics in an amount of 0.001 to 5% by weight, preferably 0.01 to 3% by weight relative to.

The cosmetic composition of the present invention is not particularly limited in the formulation, for example, it may have a formulation such as lotion, nutrition lotion, nutrition cream, massage cream, essence, pack.

In addition, in the cosmetic composition of each formulation, other components other than the above-mentioned Hwang Ryun, Yellow White, Golden, Yellow Jung and Astragalus mixed extract may be arbitrarily selected and blended according to the formulation or purpose of use of other cosmetics.

The cosmetic composition of the present invention is effective for anti-inflammatory, antioxidant, degranulation inhibition, cell proliferation, skin itching, atopic skin improvement, anti-oxidant cosmetic composition, anti-inflammatory cosmetic composition, anti-inflammatory cosmetic composition, allergy prevention and atopic skin improvement cosmetics The composition is preferred.

The composition containing the yellow lotus, yellowish white, golden, yellow and astragalus extract of the present invention has the effect of anti-inflammatory, antioxidant, degranulation suppression, cell proliferation, skin itching improvement, atopic skin improvement.

Hereinafter, the configuration and effects of the present invention will be described in detail with reference to Examples, Comparative Examples and Experimental Examples, but the present invention is not limited thereto.

In addition, Hwangnyeon, Hwangbaek, Golden, Hwangjeong and Hwanggi mentioned below were purchased from Gyeongdong Market in Seoul, South Korea, and the extraction solvent was purchased from Daejeong Chemical and Samkwang Chemical.

< Example  1> goldthread , Yellow white , Gold, Imperial  And Hwanggi  Extract manufacturer

After drying, 1.5 kg of distilled water and 3.5 kg of ethanol were added to a sample mixed with 100 g of fine yellow lotus, 100 g of yellow white, 100 g of golden yellow, 100 g of yellow sugar, and 100 g of astragalus (dry weight ratio = 1: 1: 1: 1: 1). The extract was boiled at 80 ° C. for 6 hours, extracted with 300 mesh filter paper, and allowed to stand at room temperature for 1 week. The precipitate was filtered twice with Edbentech No. 5 filter paper and Whatman GFC 150 mm filter paper. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a dry weight of 70.5 g.

< Example  2 to 6> goldthread , Yellow white , Gold, Imperial  And Hwanggi  Number by mix ratio ( water A) extract manufacturer

The extract of Examples 2 to 6 was obtained by extracting in the same manner as in Example 1, except that the sample mixing ratio (Russium: Yellow: Golden: Yellow: Sulfur) of Table 1 was used, and the results are shown in Table 1 below. Is the same as

Table 1

< Example  7 ~ 12> Depending on the extraction solvent goldthread , Yellow white , Gold, Imperial  And Hwanggi  Preparation of Mixed Extract

The composition weight ratio of rhubarb, yellowish white, golden, yellow gold and yellow group is 4: 1.5: 1.5: 1.5: 1.5, that is, 200 g: 75 g: 75 g: 75 g: 75 g, except that the solvent of Table 2 is used. Extraction was carried out to obtain the extracts of Examples 7 to 12. The results are shown in Table 2 below.

[Table 2]

< Comparative Example  1> goldthread  Preparation of Extract

100 g of rhubarb is added to 300 g of distilled water and 700 g of ethanol and extracted by boiling at 80 ° C. for 6 hours in an extractor equipped with a cooling condenser. Filtered twice. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a dry weight of 18.3 g.

< Comparative Example  2> Yellow white  Preparation of Extract

100 g of yellow and white 100 g of distilled water and 700 g of ethanol were extracted by boiling at 80 ° C. for 6 hours in an extractor equipped with a cooling condenser, filtered through 300 mesh filter paper, and allowed to stand at room temperature for 1 week. Filtered twice. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a dry weight of 16.6 g.

< Comparative Example  3> Preparation of Golden Extract

100 g of golden 100 g of distilled water and 700 g of ethanol were extracted by boiling at 80 ° C. for 6 hours in an extractor equipped with a cooling condenser, filtered through 300 mesh filter paper, and allowed to stand at room temperature for 1 week. Filtered twice. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a dry weight of 15.7 g.

< Comparative Example  4> Imperial  Preparation of Extract

100 g of yellow gold was added with 300 g of distilled water and 700 g of ethanol, and extracted by boiling at 80 ° C. for 6 hours in an extractor equipped with a cooling condenser, filtered through 300 mesh filter paper, and allowed to stand at room temperature for 1 week. Filtered twice. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a dry weight of 14.2 g.

< Comparative Example  5> Hwanggi  Preparation of Extract

100 g of Astragalus was poured into 300 g of distilled water and 700 g of ethanol, extracted by boiling at 80 ° C. for 6 hours in an extractor equipped with a cooling condenser, filtered through 300 mesh filter paper, and allowed to stand at room temperature for 1 week. It was filtered twice with. After concentrating at 50 ° C. using a vacuum concentrator, the resultant was dried using a spray dryer (manufactured by BUCHI Co., Ltd.) to obtain a dry weight of 10.3 g.

< Example 13 > goldthread , Yellow white , Gold, Imperial  And Hwanggi  Preparation of lotion using mixed extract

1 kg of the lotion (skin lotion) containing the yellow lotus, the yellowish white, golden yellow, yellow gold and yellow sugar mixed extract powder (Example 2) was prepared using the following preparation method.

Materials 2, 3, 4, and 8 were sequentially added to materials 11 in the table, followed by stirring to dissolve the materials. After dissolving material No. 5 in another container by heating to about 60 ° C., the material No. 10 was added to dissolve, and the solution was added to the solution containing material No. 11. Finally, 1,6,7,9 material was added and stirred sufficiently, and aged at room temperature for 3 days to prepare the title lotion.

< Example  14>. goldthread , Yellow white , Gold, Imperial  And Hwanggi  With mixed extract Nutrition lotion My article

1 kg of nutrient lotion containing yellow lotus, yellowish white, golden, yellow, and yellow berry mixed extract powder (Example 2) was prepared using the following preparation method in the amounts shown in the following table.

In the above table, 10, 11, 13, 16, the mixture was stirred and heated to 80 ~ 85 ℃ while mixing and stirring the emulsifier 2, 3, 4, 5, 6, 7, 8, 9, Material 12 was dissolved by heating to 80 ~ 85 ℃. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add material 15 and cool to 45 ℃, add material 14, add material 1 at 35 ℃, and cool to 25 ℃ Aging for 3 days produced the title nutrition lotion.

< Example  15> goldthread , Yellow white , Gold, Imperial  And Hwanggi  With mixed extract Nutrition Serum  Produce

A nutrient serum containing rhubarb, yellowish white, golden, yellowish yellow, and yellowish mixed extract powder (Example 2) was prepared by using the following preparation method using 1 kg of the following content.

2, 3, 5, and 8 in the above table was mixed and stirred and heated to 40 ~ 45 ℃ to the manufacturing unit, the emulsifier was activated and the 4, 6, 7 material was added to the manufacturing unit and emulsified. After emulsification, the mixture was cooled to 35 ° C. while stirring using a stirrer, 1 time material was added, cooled to 25 ° C., and aged at room temperature for 3 days to prepare the title nutrition serum.

< Example  16> goldthread , Yellow white , Gold, Imperial  And Hwanggi  Essence Preparation Using Mixed Extract

Essence containing the yellow lotus, yellowish white, golden, yellow, and Astragalus mixed extract powder (Example 2) was prepared by using the following preparation method in the content of the following table 1kg.

Nonionic amphiphilic lipids were prepared by homogenizing materials 2, 3, 4, 5 and 6 in the above table at a constant temperature. After mixing the nonionic amphiphilic lipids with materials 1, 7, 8 and 14 and homogenizing it at a constant temperature, it is passed through a microfluidizer, and then the material is heated to 50 to 60 ° C and slowly added to homogenize it. The microfluidizer was passed again. Thereafter, 10, 11, 12, and 13 materials were added to disperse and stabilized, and aged for 3 days at room temperature to prepare the title essence.

< Comparative Example  6 to 11> using a single extract powder Nutrition Serum  Produce

1 kg was prepared using the following preparation method as a nutrient serum in the cosmetics containing the yellow lotus, yellowish white, golden, yellow, and astragalus extract powder, respectively.

2, 3, 5, and 8 in the above table was mixed and stirred at 40 ~ 45 ℃ heated to the manufacturing unit, and then actuated the emulsifier and 4, 6, 7 was added to the manufacturing unit and emulsified. After the emulsification is completed using a stirrer while cooling to 35 ℃ and 10, 11, 12, 13, 14 materials were added to each and then cooled to 25 ℃ and aged for 3 days at room temperature, Comparative Examples 6 to 11 Prepared.

< Experimental Example  1> Confirm cell nontoxicity and cell proliferation effect

The cell proliferation effect using the cell culture of the extract dry powder prepared in Example 2, Comparative Examples 1, 2, 3, 4, 5 was measured.

1) Experimental method

Cytotoxicity and proliferation experiments were carried out in the following manner. Incubate the cells (fibroblast, 3T3) in 96-well microplates at 5,000 cells / well and incubate in a thermostat for 30 minutes, and sample is 0.01, 0.05, 0.1, 0.5, 1.0 (%, W / V) for each concentration. )) And incubated for 72 hours. Thiazoline blue was administered and further cultured for 4 hours. After discarding all the culture medium, acidic isopropanol was added to each well of the microplate, and stirred for 5 minutes, and then absorbance was measured at 570 nm. As the control group, 10% Fetal Bovine Serum (FBS) medium was used as the sample injection amount to co-culture as an optimal condition for cell growth. .

2) Experiment result

Cell proliferation was calculated by the following equation 1, the results are shown in Table 3 below.

[Equation 1]

[Table 3]

As shown in Table 3, when the cell proliferation at 100% cell growth conditions was 100%, the extracts of the present invention, each of the single extract and the mixed extracts of the yellow lotus, the yellow white, the golden, the yellow, and the yellow, respectively, were cell-toxic and excellent. It was confirmed that there is a cell proliferation effect.

< Experimental Example  2> Free radical  Confirmation of scavenging effect (checking antioxidant effect)

Free Radical scavenging effect was measured for Example 2 and Comparative Examples 1, 2, 3, 4, and 5, and Quercetin (manufacturer: Sigma-Aldrich) was used as a comparative material.

1) Experimental method

Experiments were performed using the DPPH (1,1-diphenyl-2-picryl-hydrazyl) method (Blois. MS Nature 181, 1190, 1958), and DPPH and quercetin were used by SIGMA. It was. To 1 ml of 0.2 mM DPPH methanol solution, ethanol solutions of various concentrations (5, 10, 20, 30 ppm) of Example 2, Comparative Examples 1, 2, 3, 4, 5 and quercetin were added and stirred well at room temperature. The reaction was carried out for 10 minutes at. Then absorbance was measured at 517 nm, where purified water was used instead of each sample in a blank test.

Using the following formula 2 to obtain the free radical scavenging effect and the results are shown in Table 4 below.

[Equation 2]

2) Experiment result

Table 4

As can be seen in Table 4, the free radical 50% scavenging concentration (SC ₅₀ ) of Example 2 is 15.14 ppm, which is somewhat lower than that of 10.19 ppm of quercetin, but quercetin is a single substance and extracted to obtain the pure substance. Considering the stability and the cost of use, it can be seen that the free radical scavenging effect of the mixture of sulfur, yellow and white, golden, yellow and yellow extracts is better and more effective in terms of use and production cost.

< Experimental Example  3> Lipoxygenase  Check the inhibitory effect

For Example 2, Comparative Examples 1, 2, 3, 4, 5, the effect of inhibiting lipoxygenase activity was measured, and quercetin was used as a comparative substance.

1) Experimental method

The experiment was performed using the TBARS method, and linoleic acid, lipoxygenase, thiobarbitulic acid, and quercetin used in the experiment were used by SIGMA. . To 1 ml of 1 ml linoleic acid, 0.05 ml of various concentrations (1, 5, 10, 20, 30 ppm) of Example 2, Comparative Examples 1, 2, 3, 4, 5 and quercetin were added and 0.95 ml of lipoxygenase After administration, the mixture was stirred well and reacted at 25 ° C for 10 minutes. 0.5 ml of trichloroacetic acid was added, 1 ml of thiobarbiturlic acid was added, and then heated for 10 minutes, and then cooled in ice water for 2 to 3 minutes to terminate the reaction. 2 ml of butanol was added to the reaction solution, followed by centrifugation at 4000 g for 5 minutes, and the absorbance was measured at 535 nm. At this time, purified water was used instead of each sample as a control.

The following formula 3 was used to determine the effect of inhibiting lipoxygenase activity and the results are shown in Table 5 below.

[Equation 3]

[Table 5]

As can be seen in Table 5, the lipoxygenase 50% activity inhibitory concentration (IC ₅₀ ) of Example 2 is 9.53 ppm, which is slightly lower than that of 9.46 ppm of quercetin, but quercetin is a single substance and extracted from the pure substance. Considering the stability and cost of the use and use, it can be seen that the inhibitory effect of lipoxygenase activity of the mixture of yellow, yellow, yellow, golden, yellow, and astragalus is better, and more effective in terms of use and production cost.

< Experimental Example  4>. Of hyaluronidase  Confirmation of activity inhibitory effect

In Example 2, Comparative Examples 1, 2, 3, 4, 5, the effect of inhibiting hyaluronidase activity was measured.

1) Experimental method

By applying the Morgan-Elson method, the final enzyme activity of hyaluronidase was 400NF unit / ml, the final concentration of Hyaluronic Acid was 0.4mg / ml, and the active compound Compound 48/80 buffer solution (0.1) Mg / ml) was used to measure Hyaluronidase activity. The sample was dissolved in 0.1 M acetate buffer (pH 3.5) to prepare a sample solution. The control group was extracted with green tea extract (70% (wt / wt) functional butylene glycol at 85 ° C. for 2 hours instead of the sample solution). And dried.)

For each blank, buffer was used instead of enzyme solution.

The following formula 4 was used to determine the effect of inhibiting hyaluronidase activity and the results are shown in Table 6 below.

[Equation 4]

Table 6

As can be seen in Table 6, the hyaluronidase 50% activity inhibitory concentration (IC ₅₀ ) of Example 2, which is an extract of the present invention, was 1.90%. Example 2, Comparative Example 3, which is a single golden extract, Comparative Example 4, which is a single yellow gold extract, and Comparative Example 5, which is a single yellow gold extract, showed much better effects. In addition, the extract of the present invention showed a very excellent effect of inhibiting the hyaluronidase activity than 2.86% of the green tea extract of the comparative example.

< Experimental Example  5>. Immune cell Degranulation  Inhibitory effect

The degranulation effect of immune cells was measured for Example 2 and Comparative Examples 1, 2, 3, 4 and 5.

1) Experimental method

Example 2 of the present invention was treated with a RBL-2H3 cell line (Rat mast cell line) to confirm the effect of inhibiting degranulation by antigen.

RBL-2H3 cell line was obtained from Eagle's medium (Dulbeccos' modified Eagle's medium, DMEM) containing 1% Fetal bovine serum and L-glutamine. Incubated in a 37 ° C, 5% CO ₂ incubator, cells with adherence were suspended using trypsin-EDTA solution, separated and recovered and used for the experiment.

After activating the RBL-2H3 cell line with an activator (Dinitrophenol-conjugated human serum albumin, (DNP-HSA) -specific IgE), the activated cells were treated with 50 ng / mL of antigen (DNP-HSA). Comparative Examples 1, 2, 3, 4, and 5 were treated with 0, 5, 25, and 125 μg / ml, respectively, and incubated for 60 minutes. Then, the amount of β-hexosaminidase secreted into the medium was measured using an ELISA reader. Inhibition of the amount of β-hexosaminidase secretion means preventing degranulation of immune cells. The results are shown in Table 7.

Table 7

As can be seen in Table 7, the degranulation 50% inhibitory concentration (IC ₅₀ ) of the immune cells of Example 2 was 39.87 ug / ml. It showed a much better effect on degranulation of immune cells than Comparative Example 3 which is a single golden extract, Comparative Example 4 which is a yellow single extract and Comparative Example 5 which is a single yellow extract.

< Experimental Example  6> Confirmation of atopic skin improvement effect

Example 15 The clinical evaluation of the atopic skin improvement effect on cosmetics was measured.

Example 15 In order to evaluate the improvement effect of the atopic skin of the cosmetics, the following experiments were carried out on 60 patients treated with atopic dermatitis in a clinic and a hospital. The test sample was applied to the whole body by dividing the right side and the left side by the double-blinded test, but the use of other moisturizers that could affect the effect of the test sample was prohibited. The effects after 1, 2, and 8 weeks of application of the test samples were evaluated by SCORAD: SCORAD: SCORAD Atopic Dermatitis (TA) method, and the results are shown in Table 6 below.

1) Criteria

① Extent criteria: Area = damage area / 100

② intensity criteria

   -Erythema (1/2/3)

   -Edema (1/2/3)

   -Exudate (1/2/3)

   -Skin peeling (1/2/3)

   Taekwondo degree (1/2/3)

   -Drying degree (1/2/3)

Subjective criteria

   -Itching (1-10)

   Insomnia (1-10)

* Score calculation = (accuracy criterion / 5) + (strength criterion / 7) + subjective criterion

Table 8

Referring to Table 8, the cosmetics of Example 15 containing a mixture of yellow lotus, yellowish white, golden, yellow and astragalus simply contained Comparative Example 6 containing water and Comparative Examples 7, 8, 9, containing a single extract. Compared with 10 and 11, it can be seen that the atopic skin improvement effect is very excellent. In particular, the composition of the present invention shows an excellent improvement effect of 90% or more after 8 weeks.

On the other hand, in order to examine the atopic dermatitis relief effect felt by the caregiver, after using the cosmetics of Example 15 and Comparative Examples 6, 7, 8, 9, 10, 11 for 1 month, the parent of the user according to the following criteria It is shown in Table 9 to evaluate the degree of improvement of the overall dermatitis and the side effects caused by the test sample with the satisfaction of itching, erythema change, dry skin.

(Criteria)

Table 9

As shown in Table 9, the cosmetic composition of Example 15 according to the present invention is a skin itching effect, erythema improvement effect, dry skin improvement effect for children with atopic skin as a test subject is purified water or a single component extract formulation It was superior to Examples 6-11.

Claims (10)

A mixture extract of a mixture of rhubarb, yellowish white, golden, yellow, yellow and sulfuric acid with one or more solvents selected from water, alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerin, ethylene glycol, propylene glycol, butylene glycol, and hydrates thereof. The mixed extract of claim 1, wherein the solvent is at least one selected from water, anhydrous alcohols having 1 to 4 carbon atoms, or hydrous alcohols thereof. The mixed extract according to claim 1, wherein the ratio of the mixture of yellow lotus, yellow white, golden, yellow and yellow, is dry weight ratio of 4: 1.5: 1.5: 1.5: 1.5. The mixed extract of claim 1, wherein the extract is extracted at 50 to 100 ° C. Cosmetic composition containing the extract of any one of Claims 1-4. The cosmetic composition according to claim 5, wherein the mixture of the yellow lotus, the yellow white, the golden, the yellow, and the yellow sugar is contained in an amount of 0.001 to 5% by weight based on the total dry weight of the cosmetic composition. The cosmetic composition according to claim 5, wherein the cosmetic composition has a formulation of softening cream, nutrient cream, massage cream, nutrition serum, essence, and pack. The cosmetic composition according to claim 5, wherein the cosmetic composition is for allergy prevention and atopic skin improvement. The cosmetic composition according to claim 5, wherein the cosmetic composition is for antioxidant. The cosmetic composition according to claim 5, wherein the cosmetic composition is for anti-inflammatory.