KR101135172B1 - A composition for the prevention, improvement or treatment of acne vulgaris comprising the mixture of extract of Phellodendron amurense Rupr, Houttuynia cordata, Paeonia lactiflora Pall, Agrimonia pilosa Ledeb, and Glycyrrhiza uralensis Fisch as an effective ingredient - Google Patents

Abstract

The present invention is for acne prevention, improvement or treatment of a mixed extract of Phellodendron amurense Rupr, Houttuynia cordata, Peony (Paeonia lactiflora Pall), Agrimonia pilosa Ledeb and Glycyrrhiza uralensis Fisch It relates to a composition. The mixed extract has antimicrobial activity against Propionibacterium acnes (P.acnes), Staphylococcus epidermidis (S. epidermidis) and Malassezia furfur (M. furfur) related to acne, so that the composition containing the mixed extract as an active ingredient prevents acne. It can be usefully used for the purpose of improvement or treatment.

Description

Composition for the prevention, improvement or treatment of acne vulgaris comprising the mixture of extract of Phellodendron amurense Rupr, Houttuynia cordata, Paeonia lactiflora Pall, Agrimonia pilosa Ledeb, and Glycyrrhiza uralensis Fisch as an effective ingredient}

The present invention relates to a composition for preventing, improving or treating acne, which contains a mixed extract of yellowish white, eoseongcho, Seonhakcho, peony and licorice as an active ingredient.

Acne (acne vulgaris) is a skin disease that occurs between the ages of 15 and 40, and is known to be a common disease, especially in puberty. Mainly in fur glands characterized by lesions such as whiteheads or blackheads, cotton, papules, pustules, nodules, etc. It is defined as a chronic inflammatory disease that occurs. Acne is a common type of acne, commonly known as acne, also known as vulgar acne, characterized by a mixture of typical cotton rash and various types of rash. Acne keloidalis is characterized by large abscesses, pus pockets, and inflammatory nodules from which pus-like discharges are scattered. These are the most severe forms of acne. About a week before menstruation begins, premenstrual acne, which is aggravating acne, and neonatal acne (preadolescent acne) caused by progesterone from the mother during fetal periods, which stimulates sebaceous glands. In addition, acne around the mouth, adult acne and the like. Although teenagers are known to have various levels of acne in 90% of cases, air pollution, drug abuse, etc. have recently occurred regardless of age. A variety of factors have been shown to affect the development of acne, among which four major factors, namely abnormal hair follicle aberration, increased sebum secretion, the growth of bacteria represented by acne bacteria Propionibacterium acnes (P. acnes), Induced inflammation is considered to be important (Oh Sung Moon, a search for natural antimicrobial agents against acne-causing microorganisms from medicinal plants, Master's thesis, Yonsei University, 2006).

In the past, the occurrence of acne was considered a natural growth process or constitution, but recently, an increasing number of people are interested in and want to actively manage or treat it. Most skin problems do not interfere with life, but they have a psychological effect on interpersonal relationships and daily life. In particular, acne can be more serious than other medical problems because, unlike other diseases, it can be exposed and sometimes touched directly. Moreover, it is one of the skin problems many modern people suffer from because it is a disease that persists not only in adolescence but also as an adult (Oh, Hye-young, et al.

The mechanism of the development of acne is as follows. At puberty, both male and female sex hormones become active, stimulating the sebaceous glands attached to the hair follicles, and as a result, the sebaceous glands become larger and secrete more sebum. At the same time, the pores, ie, the stratum corneum at the pores entrance, become thicker and have higher adhesion, resulting in narrowing or clogging the pores. As a result, sebum accumulates in the hair follicles, and bacteria living in the hair follicles multiply and develop into inflammation. Androgen, a male hormone that fundamentally stimulates sebaceous glands, and breeds in the fur gland, breaks down sebum, produces free fatty acids, and acts as P. acnes (Neuro-Hun et al., Sebum production and acne, Korean Society of Skin Barriers) (1), 56-60, 2008).

In addition to P. acnes, bacteria such as Staphylococcus epidermidis (S. epidermidis) and Malassezia furfur (M. furfur) have been reported as bacteria that can cause skin irritation. On the other hand, P. acnes is a representative bacterium isolated from acne lesions, but other bacteria such as S. epidermidis have been reported to be isolated. The bacteria of the genus Malassezia are long-lived fungi that can cause skin infections under certain conditions and can be opportunistic pathogens in transplant patients. Active Screening, Korean Journal of Microbiololgy and Biotechnology 34 (3), 265-272, 2006).

P. acnes is a Gram-positive anaerobic bacterium, 0.3-1.3㎛ × 1-10㎛, part of the cells is swelled, the fungi are sharp, polymorphic, and have a V-shape. It is one of the normal flora detected in the sebaceous glands of normal adult sebaceous gland or around the pores with high sebum secretion. Sometimes. P. acnes is the main nutrient for sebum, and triglyceride, the main component of sebum, is broken down into fatty acids and glycerol, and the free fatty acids are severely irritating to the skin, causing inflammation of cells around the hair follicle wall and pores. In addition, the epithelial cells stimulated by the fungus increase the production of keratinocytes, resulting in stagnant hyperkeratosis, forming microenvelopes, which are the early stages of acne, and the breakdown of keratin and sebum. The action of hydrolase secreted by phagocytic leukocytes ruptures the hair follicle wall and releases the hair root contents into the dermis, accelerating the inflammatory response (Isolation, Identification and Biochemical Specific Studies of Propionibacterium acnes from Acne Lesions, etc. , Korean Journal of Apparel Industry 8 (5), 571-576, 2006).

S. epidermidis is one of the genus Staphylococcus belonging to the Gram-positive bacteria. Colonies of S. epidermidis are usually small, white to yellow, and grow to about 1-2 mm in diameter after overnight culture. Occurs frequently on human and animal skin, and also on mucous membranes. It grows by aerobic breathing or fermentation and can survive anaerobic. It is sensitive to the antibiotic Novobiocin, but resistant to various antibiotics such as penicillin and methicillin. S. epidermidis is generally a non-pathogenic bacterium, but is a major cause of infection in the immune system and indwelling catheterization. Many varieties adhere to the surface of medical prostheses to form biofilms (Kang Young-sik et al., Epidemiologic study of Staphylococcus epidermidis keratitis, Journal of the Korean Ophthalmology 43 (4), 665-671, 2002).

Malassezia furfur (M. furfur) is a polymorphic lipophilic microorganism with a thick, multi-layered cell wall. The colonies are small, yellowish-brown, with a clear border. Malassezia furfur belongs to yeast and is usually on the surface of animal skin, including humans. Infection can result in hypopigmentation of the back or chest area. To survive, fat is needed, so most of the hair, face and upper body are commonly present in the sebaceous glands. When the growth rate is too fast, the natural regeneration of the cells is disturbed and scratching causes dandruff.

On the other hand, the mixed extract included in the composition of the present invention is a mixed extract of yellowish white, eoseongcho, Seonhakcho, peony, licorice, each pharmacological action is as follows.

Hwangbaek is a dried bark of a yellow wall or other similar plant belonging to the rutaceae family, and it is also called Hwangkyungpi, and its scientific name is Phellodendron amurense Rupr. It is used for conjunctivitis, pancreatitis, chronic colitis, and bacteria, and it is used as an herbal medicine for inflammation, pneumonia, bone tuberculosis, colds, fungicides, anti-inflammatory drugs, and as an antipyretic medicine for other infectious diseases. Isolation of Melanin Biosynthesis Inhibitor from Hwangbaek Lee, et al., Korean Journal of Pharmacognosy 38 (4), 387-393, 2007).

Eoseongcho is a perennial plant of the family Saururaceae and refers to the ground part of the flowering period of Houttuynia cordata Thunb in Korea, and its scientific name is Houttuynia cordata. As a perennial traditional medicinal plant, it has been used as a disinfectant, diuretic, antipyretic, antibacterial, antiviral, and anti-inflammatory agent for a long time in countries such as Korea, Japan and China. Efficacy in the treatment of diuresis, detoxification, cardiac, antipyretic, drainage, antibacterial, anticancer and sinusitis, pneumonia, arthritis, eczema, syphilis, angina, cholelithiasis, kidney disease, gout, hemorrhoids, diabetes, hypertension, and disease Taken for cosmetic purposes (Cha Jae-young, et al., Effects of Chaga and Echo-fermented Fermentation Compositions on Growth of Human Gastric Cancer AGS and Colon Cancer HCT-15 Cells, Korean Society for Applied Biochemistry 47 (2), 202-207, 2004).

The peony is also known as a perennial herb and is a perennial herbaceous herb belonging to the Ranunculuaceae family. The name is Paeonia lactiflora Pall. It is the most widely used medicinal crop after ginseng and tangui in Korea, and it is widely used as a raw material of herbal medicines such as Saphwatang, Samultang, etc. Peony roots are prescribed for the purpose of treatment of acupuncture, pain and pain in the oriental medicine, mainly for control of amenorrhea, trauma, nasal bleeding, inflammation, boils and wounds, and pain relief in the chest and rib areas. come. The bioactive components of the peony roots were separated from the monoterpene glucoside compounds, including paeoniflorin and albiflorin, and several phenolic compounds. Sejong Kim et al., Separation and Identification of Bioactive Substances from Peony Leaves and Stems, 15 (1), 6-11, 2007).

Seonhakcho is a perennial root of the genus Rosaceae, and it is called yongah, yongcho, hwangcho, huangyongcho, jisuncho, and straw herb. The scientific name is Agrimonia pilosa Ledeb. Since ancient times, it has been widely used as folk remedies, green juice, lung cancer, liver cancer, esophageal cancer, tumors, pain relief, topography, branches, hematopoiesis, hematuria, uterine bleeding, and medicinal purposes. Recently, antimicrobial activity and antihypertensive activity have been reported (Bae Ji-Hyun, et al., The effect of Seonhakcho extract on the proliferation of food poisoning-inducing bacteria, Korean Journal of Nutrition, 38 (2), 112-116, 2005).

Licorice is a perennial herb belonging to the legume (Leguminosae) family and its scientific name is Glycyrrhiza uralensis Fisch. Glycyrrhizin, a sweet ingredient, is used as a sweetener, and it is known to treat various diseases, antibacterial action and excellent antioxidant activity. Root is used as a medicinal herb, known to act on the heart, lungs, gastric and jugular vein, and is known to be effective in oozing, wetting, and growth. It is also known to alleviate pain caused by rapid tension of muscles or tissues, increase weight, increase white blood cells, diuretic effect, and anti-inflammatory effect (Pakjaja, etc., antibacterial and antioxidant activity of licorice and spice extract, Korean Journal of Food and Cookery Science 23 (6) , 793-799, 2007).

Acne treatments on the other hand inhibit sebum overproduction, prevent hair follicle wall hyperkeratosis, It is developed in terms of inhibition of proliferation of P. acnes and prevention of inflammatory reactions. However, currently used erythromycin, isotretinoin, benzoyl peroxide, vitamin A acid, triclosan and azelaic acid Drugs are known to have many problems such as cough, mucous membrane dryness, teratogenicity, blemishes or thrombosis, side effects such as the emergence of antibiotic resistant strains or recurrence when discontinuing treatment. The present inventors have found a mixed composition of natural products with less side effects and effective in the treatment of acne, and completed the present invention.

The present invention is to provide a composition for the prevention, improvement or treatment of new acne using a natural herbal extract without toxicity.

The present inventors have found that the mixed extracts of Hwangbaek, Eoseongcho, Seonchocho, Peony and Licorice show excellent antimicrobial activity against acne-related bacteria such as P. acnes. The present invention has been completed regarding a composition for preventing, improving or treating acne.

The mixed extract of the above five extracts showed excellent antimicrobial activity against microorganisms related to acne and skin hygiene. In addition, when the five extracts were used alone, the effect was better when mixed, and the antibacterial activity was slightly different depending on the mixing ratio. Thus, the mixed use of the five extracts found that the effect is superior to the single use to complete the present invention.

Accordingly, the present invention provides a composition for preventing and treating acne, which contains a mixed extract of baekbaek, eoseongcho, peony, Seonhakcho and licorice as an active ingredient.

The mixed extracts of Hwangbaek, Eoseongcho, Peony, Seonhakcho and Licorice according to the present invention showed little toxicity and excellent antimicrobial activity against P. acnes. Using such a mixed extract will be able to prevent, improve or treat acne by inhibiting excessive growth of epidermal bacteria, one of the causes of acne.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The present invention provides a composition for the prevention, improvement or treatment of acne, containing a mixed extract of baekbaek, eoseongcho, peony, Seonhakcho and licorice as an active ingredient.

Specific contents for carrying out the present invention for achieving the above object are as follows.

In the composition of the present invention, it is preferable that the mixed extract is a mixture of each of the extracts of the baekbaek, eoseongcho, peony, Seonhakcho and licorice.

In the present invention, the extracts of the baekbaek, eoseongcho, Seonhakcho, peony and licorice may be extracted or commercially available according to methods well known in the art. In addition, baekbaek, eoseongcho, Seonhakcho, peony and licorice can be used without limitation, such as cultivated or commercially available, washed clean.

In the composition according to the present invention, the site used in the preparation of the extract of the yellow white is a bark, and the site used in the preparation of each extract of Eoseongcho, Peony, Seonchocho and Licorice, respectively, leaves, stems, roots or mixed two or more from them Can be used. Preferably, the peony and licorice use its roots, and the Echo and Seonhakcho use leaves or stems.

In the composition of the present invention, the extract of each of the yellowish white, eoseongcho, peony, Seonhakcho and licorice is preferably an extract of a solvent selected from the group consisting of water, C ₁ -C ₄ alcohols, organic solvents and mixtures thereof, More preferably, an extract of a solvent selected from the group consisting of water, C ₁ to C ₃ alcohols and mixtures thereof is used, and even more preferably water extract is used.

In the composition of the present invention, the mixing ratio of the mixed extract of the yellowish white, eoseongcho, Seonhakcho, peony and licorice is as follows.

The mixed extract according to the present invention is less than 100% by weight of 0.01% by weight or more, less than 100% by weight from 0.02% by weight or more of Echochocho extract, less than 100% by weight from 0.01% by weight or more of Peony extract, 0.02% by weight or more of Seonchocho extract And less than 100% by weight and licorice extract from 0.04% or more by less than 100% by weight.

The mixed extract may include 1 to 70% by weight of the baekbaek extract, 1 to 60% by weight Eochocho extract, 1 to 60% by weight peony extract, 1 to 50% by weight of Seonchocho extract and 1 to 50% by weight licorice extract.

It is preferable to include 15 to 70% by weight of baekbaek extract, 1 to 50% by weight Eochocho extract, 1 to 60% by weight peony extract, 1 to 50% by weight Seonchocho extract and 1 to 30% by weight licorice extract.

More preferably, it comprises 25 to 60% by weight of the baekbaek extract, 15 to 45% by weight Eochocho extract, 1 to 35% by weight peony extract, 1 to 35% by weight Seonchocho extract and 1 to 15% by weight licorice extract.

Most preferably, it may include 45 to 60% by weight of baekbaek extract, 15 to 45% by weight of Echochocho extract, 15 to 20% by weight peony extract, 1 to 10% by weight of Seonchocho extract and 1 to 10% by weight licorice extract, In particular, 50% by weight of the white extract, 20% by weight Eochocho extract, 20% by weight peony extract, 5% by weight of Seonhakcho extract and 5% by weight licorice extract.

On the other hand, in the mixing ratio comprising 15 to 70% by weight of the whitening extract, 1 to 50% by weight of Eochocho extract, 1 to 60% by weight of peony extract, 1 to 50% by weight of Seonchocho extract and 1 to 30% by weight licorice extract, The mixing ratio of the remaining portions except for the mixing ratio including 25 to 35% by weight of the extract, 25 to 35% by weight of Echochocho extract, 1 to 10% by weight of Peony extract, 25 to 35% by weight of Seonchocho extract and 1 to 10% by weight of licorice extract Preferably used.

In the composition of the present invention, the mixed extract may be sterilized by irradiation with radiation, while a gamma ray source may be used as the radiation source, and ⁶⁰ Co may be used as the gamma ray source.

Hereinafter, in the composition of the present invention, a method for producing a mixed extract of sulfur white, fish vinegar, sunhakcho, peony and licorice will be described.

In the present invention, the extracts of the baekbaek, eoseongcho, Seonhakcho, peony and licorice may be extracted or commercially available according to methods well known in the art. In addition, baekbaek, eoseongcho, Seonhakcho, peony and licorice can be used without limitation, such as cultivated or commercially available, washed clean.

In the mixed extract according to the present invention, the extracting solvent used in the preparation of the extracts of the yellowish white, eoseongcho, peony, sunhakcho and licorice is selected from the group consisting of water, C ₁ -C ₄ alcohol, organic solvents and mixtures thereof A solvent may be used, and preferably, a solvent selected from the group consisting of water, C ₁ to C ₃ alcohols, and mixtures thereof may be used, and more preferably water may be used as an extraction solvent.

In the composition of the present invention, each of the extracts for preparing the mixed extract of the sulfur white, Eochocho, Seonchocho, Peony and Licorice are ground to an appropriate size by crushing the dried sulfur white, Echochocho, Seonchocho, Peony and Licorice into an extraction container An amount of water, an alcohol or a mixture thereof, or an organic solvent may be added thereto, followed by extraction at a suitable temperature for 12 hours or more, and then filtered through a filter paper or the like to obtain each extract according to the present invention. As the organic solvent, it is preferable to use ethyl ether, chloroform, ethyl acetate or a mixed solvent thereof.

On the other hand, in the use of water as the extraction solvent, it is preferable to use tertiary distilled water, it is preferable to heat the heated bath for 12 to 72 hours at a temperature of 50 to 90 ℃, more preferably 60 to 60 when extracted with water The bath is heated for 24 to 72 hours at a temperature of 80 ° C.

In the composition of the present invention, the mixed extract may be sterilized by irradiating the radiation, while the mixed extract may be irradiated, or may be mixed after irradiating the respective extracts before mixing. .

On the other hand, a gamma ray source may be used as the radiation source, and ⁶⁰ Co may be used as the gamma ray source.

In the composition of the present invention, the mixed extract may be used for the purpose of preventing, improving or treating acne by having antimicrobial activity against P. acnes, M. furfur and S. epidermidis.

The composition of the present invention having a prophylactic, ameliorating or treating effect of acne includes the extracts of the yellow baekbaek, eoseongcho, peony, sunhakcho and licorice alone or further comprises one or more pharmaceutically acceptable carriers, excipients or diluents Thereby can be formulated as a pharmaceutical composition by conventional methods.

As used herein, "pharmaceutically acceptable" refers to a composition that is physiologically acceptable and that, when administered to a human, typically does not cause an allergic reaction such as gastrointestinal disorders, dizziness, or the like. In addition, the pharmaceutical composition of the present invention formulated as described above may be administered for the purpose of preventing, ameliorating or treating allergic skin diseases through a suitable route of administration. Suitable routes of administration may include several routes including oral, transdermal, subcutaneous, intravenous or intramuscular.

In the pharmaceutical composition of the present invention, the pharmaceutical composition may be formulated into a preparation for oral or parenteral administration according to the selected route of administration, and when formulated, the commonly used fillers, weights, binders, wetting agents, It is prepared using diluents or excipients such as disintegrants and surfactants. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose, and the like. (Sucrose) or lactose (Lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups, and may include various excipients such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include external skin preparations. When the composition for providing the efficacy of preventing, improving or treating acne of the present invention is an external preparation for skin, the formulation is not particularly limited. For example, the formulation may be a lotion, ointment, gel, cream, patch or spray formulation. It can manufacture.

In the pharmaceutical composition of the present invention, the mixed extracts of the baekbaek, eoseongcho, peony, Seonhakcho and licorice are preferably included in an amount of 0.01 to 90% by weight based on the total weight of the pharmaceutical composition. However, the composition as described above is not necessarily limited thereto, and may vary according to the condition of the patient, the type of disease, and the degree of progression.

In the pharmaceutical composition of the present invention, the preferred dosage of the mixed extract of the yellow, yellow, peony, sunhakcho and licorice is dependent on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and duration, Can be appropriately selected. However, for the desired effect, it is recommended to administer at 0.001 mg / kg to 10 g / kg per day. The administration may be carried out once a day or divided into several doses. Thus, the dosage amounts are not intended to limit the scope of the invention in any manner.

The pharmaceutical composition according to the present invention can be administered to mammals such as rats, mice, livestock, humans by various routes. All modes of anticipated administration are possible and can be administered, for example, by oral, dermal application, rectal, or intravenous, intramuscular, subcutaneous, intrauterine dural or Intracerebroventricular injection.

The pharmaceutical composition according to the present invention may be used alone or in combination with methods using surgery, hormonal therapy, drug treatment and biological response modifiers for the prevention and treatment of acne.

In addition, the composition of the present invention may be provided in the form of a cosmetic.

The compositions of the present invention can be formulated into cosmetics in the form of common emulsion and solubilized formulations. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing a mixed extract of yellowish white, eochocho, peony, sunhakcho and licorice of the present invention, by containing a dermatologically acceptable medium or base, a topical or systemic application commonly used in the field of dermatology It can be prepared in the form.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, a cream, a skin, a lotion, a powder, a haze, a spray or a conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol further containing a compressed propellant.

In addition, the formulation as a cosmetic of the present invention, in addition to the mixed extract of sulfur white, fish vinegar, peony, Seoncho vinegar and licorice, fatty substances, organic solvents, solubilizers and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents (Foaming agent ), Fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipids It may contain adjuvants commonly used in the cosmetic or dermatological arts, such as vesicles or any other ingredients conventionally used in cosmetics. And the above ingredients may be introduced in amounts generally used in the field of dermatology.

Specific formulations as cosmetics of the composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, the present invention will be described in detail through production examples, examples and formulation examples.

However, the following Preparation Examples, Examples and Formulation Examples are provided only to specifically describe the present invention, but the scope of the present invention is not limited by these Preparation Examples, Examples and Formulation Examples.

Preparation Example 1 Preparation of Water Extract of Hwangbaek, Eoseongcho, Peony, Seonhakcho and Licorice

The dried sulfur white was pulverized, and 50 g of the ground sulfur white was added to 500 ml of tertiary distilled water, followed by heating in a bath for 25 hours at 75 ° C. This was filtered through a paper filter Watman # 2 to remove solids, and then lyophilized to obtain 1.60 g of a powder of yellow-white extract. The prepared powder was used by irradiation sterilization with 60Co gamma rays (10 kGy / H, 25 kGy), and, if necessary, the freeze-dried powder was used in a suitable solvent.

 Extract powders of 3.37 g, 1.34 g, 2.08 g, and 4.18 g were obtained from 50 g of ground powder of Eoseongcho, Peony, Seonhakcho and Licorice, respectively, using the same method as that of the extract powder of yellowish white.

Example 1 Preparation of Mixed Extracts of Yellow White, Eoseongcho, Peony, Sunhakcho and Licorice

A mixed extract obtained by mixing each of the extract powders of Hwangbaek, Eoseongcho, Peony, Seonchocho and Licorice in the weight ratio as shown in Table 1 below. Specifically, after dissolving each extract powder obtained in Preparation Example 1 in tertiary distilled water at a concentration of 20mg / ml, the weight ratio of each extract was 40% by weight of yellow white extract, 10% by weight of Echochocho extract, 30% by weight of Peony extract, Seonhakcho Aqueous solution of each extract was dissolved in distilled water so that 10% by weight of the extract and 10% by weight of licorice extract were mixed by mixing 2 ml of sulfur white water solution, 0.5 ml of Erchochori extract, 1.5 ml of Peony extract, 0.5 ml of Sunhakcho extract and 0.5 ml of licorice extract. The extract was prepared in the form of an aqueous solution.

Weight ratio of each extract component in mixed extract Example Yellow white Eoseongcho Peony Seonhakcho licorice One 40 10 30 10 10 2 30 10 40 10 10 3 30 30 30 5 5 4 40 20 20 10 10 5 50 20 20 5 5 6 20 20 50 5 5 7 20 20 40 10 10 8 20 20 20 20 20 9 10 10 10 30 40 10 5 10 5 40 40 11 40 40 10 5 5 12 30 30 5 30 5 13 40 30 5 20 5

<Examples 2 to 13> Preparation of a mixed extract of yellow white, Eoseongcho, Peony, Seonhakcho and Licorice

A mixed extract obtained by mixing each of the extract powders of Hwangbaek, Eoseongcho, Peony, Seonchocho and Licorice in the same weight ratio as those shown in Examples 2 to 13 of Table 1 was obtained in the same manner as in Example 1.

Comparative Example 1

The extract powders of the yellowish white, eoseongcho, peony, Seonchocho and licorice obtained in Preparation Example 1 were dissolved in tertiary distilled water at a concentration of 20 mg / ml to prepare the extracts of the yellowish white, eochocho, peony, Seonchocho and licorice in the form of aqueous solution.

Experimental Example 1 Antimicrobial Activity of P. acnes

Dispense the P. acnes bacterial suspension into each well of a 96-well plate, and extract each of the extracts of Examples 1 to 13 and the extracts of the yellow white, fish vinegar, peony, Seoncho vinegar and licorice obtained in Comparative Example 1 as Comparative Examples. Treatment was at a concentration of 200 μg / 200 μl. In addition, each of the same concentration was also treated in order to determine whether the methyl paraben (MP) and propyl paraben (PP) commonly used as a preservative in cosmetics is active against acne-related bacteria. 96-well plates were measured at an absorbance of 600 nm using a microplate spectrophotometer (BioTek Instrument, Vermont, USA) at 0 and 48 hours after the extract treatment. On the other hand, no treatment (labeled CON) was used as a control. After the suspension was dispensed, the absorbance value measured 48 hours after the treatment (CON) without any treatment was divided into 0 hours and the growth rate was 100 (%). The ratio of the absorbance value measured at the time point of about 48 hours after dividing by the value of 0 hour to the control group was defined as the growth rate of each group. Therefore, showing a growth rate of 100 or less means that growth is inhibited.

Growth rates for P. acnes are shown in Table 2 and FIG.

Growth rate for P. acnes Example Growth rate (%) One 71 2 69 3 64 4 62 5 60 6 73 7 75 8 72 9 82 10 89 11 64 12 64 13 65 CON 100 MP 64 PP 48 Yellowish white sole 85 Eoseongcho Sole 73 Peony alone 76 Seonhakcho alone 77 Licorice sole 92

Examples 3, 4, 5, 11, 12, 13, the mixed extract having a mixing ratio of the single extract of the best effect of the best extract of P. acnes can be seen that the inhibitory activity against P. acnes.

Experimental Example 2 Antimicrobial Activity of M. furfur

The antimicrobial activity against M. furfur was confirmed in the same manner as in Experimental Example 1.

Growth rates for M. furfur are shown in Table 3 and FIG.

Growth Rate for M. furfur Example Growth rate (%) One 84 2 77 3 76 4 78 5 77 6 82 7 83 8 84 9 104 10 106 11 86 12 75 13 79 CON 100 MP 97 PP 177 Yellowish white sole 88 Eoseongcho Sole 95 Peony alone 88 Seonhakcho alone 91 Licorice sole 98

The mixed composition having a mixing ratio except for Examples 9, 10, and 12 shows growth inhibitory activity against M.furfur similarly to MP.

Experimental Example 3 Antimicrobial Activity of S. epidermidis

In the same manner as in Example 1 was confirmed the antimicrobial activity against S. epidermidis.

Growth rates for S. epidermidis are shown in Table 4 and FIG.

Growth Rate for S. epidermidis Example Growth rate (%) One 63 2 55 3 57 4 55 5 47 6 49 7 57 8 53 9 52 10 58 11 73 12 81 13 73 CON 100 MP 54 PP 50 Yellowish white sole 84 Eoseongcho Sole 82 Peony alone 83 Seonhakcho alone 91 Licorice sole 88

Except for Example 12, it can be seen that the growth inhibitory activity against S. epidermidis is superior to that of the single extract, which is the most effective single extract.

Examples of formulations for the composition of the present invention are illustrated below.

Preparation Example 1 Preparation of Powder

The powders are prepared by mixing the ingredients shown in Table 5 below and filling them in airtight cloths.

Raw material Content (mg) Mixed extract of Hwangbaek, Eoseongcho, Peony, Seonhakcho and Licorice 20 Lactose 100 Talc 10

Preparation Example 2 Preparation of Cosmetics

<2-1> Preparation of flexible lotion

Butylene glycol, glycerin, polyoxyethylene (60) cured castor oil, betaine, citric acid, sodium citrate, and preservatives were added to the purified water, followed by stirring and dissolving. Addition of a mixed extract of sulfur white, eoseongcho, peony, Seonhakcho and licorice according to the present invention was sufficiently stirred, and then aged to prepare a flexible lotion containing the mixed extract according to the present invention. Table 6 shows the content of each component.

Raw material Content (w / w%) Mixed extract of Hwangbaek, Eoseongcho, Peony, Seonhakcho and Licorice 25.0 Butylene Glycol 3.5 glycerin 2.5 Polyoxyethylene (60) hardening castor oil 0.1 ethanol 2.5 Betaine 1.0 Citric acid 0.01 Sodium citrate 0.03 antiseptic a very small amount Spices a very small amount Purified water Balance

<2-2> Preparation of nutritional lotion

The mixed extracts of sulfur white, fish vinegar, peony, sunhakcho and licorice, butylene glycol, glycerin, carboxyvinyl polymer, arginine, preservative and purified water according to the present invention were heated with stirring at 70-75. Squalane, butylene glycol dicaprylate / dicaprate, sorbitan stearate, polysorbate 60, glyceryl stearate and stearyl glycerate mixture, which were heated by stirring at 75 to 80, were added thereto, and then stirred. While stirring at 45 degrees while adding the fragrance, the mixture was cooled to 30 and then aged to prepare a nutritious longevity containing the mixed extract according to the present invention. Table 7 shows the content of each component.

Raw material Content (w / w%) Mixed extract of Hwangbaek, Eoseongcho, Peony, Seonhakcho and Licorice 40.0 Butylene Glycol 8.0 glycerin 5.0 Squalane 10.0 Butylene Glycol Dicaprylate / Dicaprate 5.0 Sorbitan stearate 1.5 Polysorbate 60 1.0 Glyceryl Stearate 0.5 Stearyl glycyrrhetinate 0.2 Carboxy Vinyl Polymer 0.1 Arginine 0.1 antiseptic a very small amount Spices a very small amount Purified water Balance

<2-3> Preparation of Essence

Cytocyterol, polyglyceryl-2-oleate, ceramide, ceteareth-4 and cholesterol are stirred and mixed, followed by cytocyterol, polyglyceryl-2-oleate, ceramide, ceteareth-4 and cholesterol. After stirring and mixing, emulsified by adding a mixed extract of yellowish white, eoseongcho, peony, Seonchocho and licorice according to the present invention, dicetyl phosphate, concentrated glycerin and purified water, and then, when cooled to 45 while stirring, the fragrance is added. Aged and cooled to 30 to age. Carboxyvinyl polymer, xanthan gum and preservatives were added thereto, stabilized, and aged to prepare an essence containing the mixed extract according to the present invention. Table 8 shows the content of each component.

Raw material Content (w / w%) Mixed extract of Hwangbaek, Eoseongcho, Peony, Seonhakcho and Licorice 10.0 Cytostolol 1.7 Polyglyceryl-2-oleate 1.5 Ceramide 0.7 Setares-4 1.2 cholesterol 1.5 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Carboxy Vinyl Polymer 0.2 Xanthan Gum 0.2 antiseptic a very small amount Spices a very small amount Purified water Balance

1 is a graph showing the inhibitory activity against P. acnes of the mixed extract of the present invention, A to P correspond to Examples 1 to 13, respectively. MP stands for methyl paraben, PP stands for propyl paraben.

Figure 2 is a graph showing the inhibitory activity against M. furfur of the mixed extract of the present invention, A to P correspond to Examples 1 to 13, respectively. MP stands for methyl paraben, PP stands for propyl paraben.

Figure 3 is a graph showing the inhibitory activity against S. epidermidis of the mixed extract of the present invention, A to P correspond to Examples 1 to 13, respectively. MP stands for methyl paraben, PP stands for propyl paraben.

Claims (21)

Pharmaceutical composition for the prevention, improvement or treatment of acne, containing a mixed extract of baekbaek, eoseongcho, peony, sunhakcho and licorice as an active ingredient. According to claim 1, wherein the mixed extracts of the yellow white, eoseongcho, peony, Seonchocho and licorice are selected from the group consisting of water, C ₁ ~ C ₃ alcohol, and mixtures thereof, respectively. A pharmaceutical composition for preventing, improving or treating acne, characterized in that the mixture of each extract extracted using a solvent. The method of claim 1, wherein the extracts of the yellowish white, eoseongcho, peony, Seonchocho and licorice are acne prevention, improvement, characterized in that each of the extracts extracted by using each of the white, yellowish, peony, Seonchocho and licorice Or therapeutic pharmaceutical compositions. According to any one of claims 1 to 3, wherein the mixed extract is 1 to 70% by weight of baekbaek extract, 1 to 60% by weight Eochocho extract, 1 to 60% by weight peony extract, 1 to 50% by weight of Seonchocho extract and A pharmaceutical composition for preventing, improving or treating acne, comprising 1 to 50% by weight of licorice extract. The method according to any one of claims 1 to 3, wherein the mixed extract is 15 to 70% by weight of sulfur white extract, 1 to 50% by weight of Echochocho extract, 1 to 60% by weight of Peony extract, 1 to 50% by weight of Seonchocho extract and A pharmaceutical composition for preventing, improving or treating acne, comprising 1 to 30% by weight of licorice extract. According to any one of claims 1 to 3, wherein the mixed extract is 25 to 60% by weight of sulfur white extract, 15 to 45% by weight Eochocho extract, 1 to 35% by weight peony extract, 1 to 35% by weight of Seonchocho extract and licorice Pharmaceutical composition for preventing, improving or treating acne, characterized in that it comprises 1 to 15% by weight of the extract. The method according to any one of claims 1 to 3, wherein the mixed extract is composed of 50% by weight of sulfur white extract, 20% by weight of Echochocho extract, 20% by weight of Peony extract, 5% by weight of Seonchocho extract and 5% by weight of licorice extract. Pharmaceutical composition for preventing, improving or treating acne. According to any one of claims 1 to 3, The mixed extract is acne prevention, improvement or therapeutic pharmaceutical, characterized in that the sterilized by irradiating gamma-ray, yellow baekcho, eoseongcho, peony, Seonchocho and licorice extract with gamma rays Composition. The pharmaceutical composition according to any one of claims 1 to 3, wherein the mixed extract has antimicrobial activity against Propionibacterium acnes, Staphylococcus epidermidis, and Malassezia furfur. The pharmaceutical composition for preventing, ameliorating or treating acne according to any one of claims 1 to 3, wherein the composition is in the form of an external preparation for skin. The pharmaceutical composition for preventing, ameliorating or treating acne according to any one of claims 1 to 3, wherein the composition is pharmaceutically formulated. Cosmetic composition for preventing or improving acne, containing a mixed extract of baekbaek, eoseongcho, peony, Seonhakcho and licorice as an active ingredient. The method of claim 12, wherein the cosmetics include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Cosmetic composition for preventing or improving acne, characterized in that the formulation is selected from the group consisting of soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder and eye shadow. The method of claim 12, wherein the mixed extract of the yellow white, Eochocho, Peony, Seonchocho and Licorice are each selected from the group consisting of water, C ₁ to C ₃ alcohols and mixtures thereof. Cosmetic composition for preventing or improving acne, characterized in that the mixture of each extract extracted using a solvent. The method of claim 12, wherein the mixed extracts of the yellowish white, eoseongcho, peony, Seonchocho and licorice are the acne prevention or improvement, characterized in that each of the extract extracted with each of the white, eungcho, peony, Seonchocho and licorice Cosmetic composition for The method according to any one of claims 12 to 14, wherein the mixed extract is 1 to 70% by weight of sulfur white extract, 1 to 60% by weight of Echochocho extract, 1 to 60% by weight of Peony extract, 1 to 50% by weight of Seonchocho extract and Cosmetic composition for preventing or improving acne, characterized in that containing 1 to 50% by weight licorice extract. The method according to any one of claims 12 to 14, wherein the mixed extract is 15 to 70% by weight of sulfur white extract, 1 to 50% by weight of Echochocho extract, 1 to 60% by weight of Peony extract, 1 to 50% by weight of Seonchocho extract and Cosmetic composition for preventing or improving acne, characterized in that containing 1 to 30% by weight licorice extract. 15. The method according to any one of claims 12 to 14, wherein the mixed extract is 25-60% by weight of the baekbaek extract, 15-45% by weight of Echochocho extract, 1-35% by weight peony extract, 1-35% by weight of Seonchocho extract and licorice Acne prevention or improvement cosmetic composition comprising 1 to 15% by weight of the extract. The method according to any one of claims 12 to 14, wherein the mixed extract is composed of 50% by weight of baekbaek extract, 20% by weight of Echochocho extract, 20% by weight peony extract, 5% by weight of Seonchocho extract and 5% by weight licorice extract. Cosmetic composition for preventing or improving acne. The cosmetic composition for preventing or improving acne according to any one of claims 12 to 14, wherein the mixed extract is a mixture of sterilized by irradiating baekbaek, eoseongcho, peony, sunhakcho and licorice extract with gamma rays. The cosmetic composition for preventing or improving acne according to any one of claims 12 to 14, wherein the mixed extract has antibacterial activity against Propionibacterium acnes, Staphylococcus epidermidis, and Malassezia furfur.

Images