KR101516866B1 - Composition for treatment and prevention of acne comprising Gnaphalium affine D. Don extract - Google Patents

Abstract

The present invention relates to a composition for the treatment and prevention of inflammatory skin diseases, which comprises a rice crumb extract as an active ingredient. The composition of the present invention is excellent in antibacterial and anti-inflammatory effects against acne diseases or atopic dermatitis, and can be safely used in medicines and cosmetics since it has no cytotoxicity and skin side effects.

Description

TECHNICAL FIELD The present invention relates to a composition for treating and preventing acne,

The present invention relates to a composition for the treatment and prevention of inflammatory skin diseases, which comprises a rice crumb extract as an active ingredient.

Atopic dermatitis is a chronic inflammatory skin disease characterized by persistent pruritus and skin rash and chronic eczema lesions. The cause of atopic dermatitis is considered to be the main cause of the skin barrier abnormality in addition to immunological factors and environmental factors which are largely hereditary. In addition, atopic dermatitis has been reported in the absence of γ-linolenic acid in lipids and the development of atopic dermatitis due to secretion of cytokines or chemokines.

The chemokine plays an important role in the infiltration of inflammatory cells into the skin, and the CC chemokine eotaxin-1 is particularly elevated in these dermatitis lesions, which is caused by eosinophils with CC chemokine receptor 3 (CCR3) and Th2 cells Is closely related to the chemotaxis of.

Eotaxin-1 is expressed in various types of cells such as eosinophils, lymphocytes, macrophages, fibroblasts, smooth muscle cells and epithelial cells, and plays an important role in attracting eosinophils to dermatitis lesions. Eosinophils secrete highly toxic protein and free radicals that can kill microbes and parasites, but they can be very tightly regulated because they can cause serious tissue damage to the host if inappropriately activated. When these eosinophils migrate from the circulating blood to the tissue, the key substance is Eotaxin-1, and eotaxin-1 secreted from the tissues of the inflamed site binds to the CCR3 receptor on the surface of eosinophils, resulting in eosinophil migration into inflammatory lesions. Therefore, effective control of the expression of Eotaxin-1 and CCR3 can prevent skin inflammation.

Atopy is one of the major diseases that have developed in the developed countries since the 1970s. It is increasing worldwide and the prevalence is 20% of the population. In the United States, 15 million people suffer from atopy, and 16.3% of primary, middle and high school students in Korea also suffer from atopy (Ahn SY et al. , J. Skin Barrier Res. , 8, 47-51, 2006). At present, steroid preparations, topical immunosuppressants, and antihistamines are used as therapeutic agents for atopic dermatitis. However, when the drug preparations are used for a long period, symptoms such as skin thinning, discoloration of skin color, osteoporosis, arteriosclerosis, There is a problem such as deterioration.

Acne is an inflammatory disease that occurs in the hair follicle of the skin. It is caused by the interaction of the male hormone, androgens, skin keratin, sebaceous germs and bacteria. Especially, the anaerobic bacterium, Propionibacterium acnes , Is known to be the leading causative organism. Usually acne occurs mainly in puberty and is observed in 85% of adolescents. Recently, the incidence of acne in adults has increased due to stress, smoking, eating habits, and cosmetics (Anseong-gu, Diagnosis and Treatment of Acne Bible, 2006). Drugs used in the treatment of acne now have a bactericidal effect and inhibit the local application of antibiotics such as clindamycin, tetracycline and erythromycin, which inhibit the production of free fatty acids, acne proliferation and inflammatory responses (Retinoid), which is a modified form of vitamin A, has been used. However, when the acne remedy is used for a long period of time, there is a problem such as a bad feeling due to a salt, hypertriglyceridemia and tolerance.

As such, treatment of atopy or acne by a single substance has side effects, and thus, plant extracts having relatively few side effects have been searched for substances for treating skin inflammation such as atopy and acne.

For example, Korean Patent Registration No. 10-1027111 discloses a cosmetic composition for skin irritation mitigation and anti-inflammation containing a fermented jujube leaf extract, Korean Patent Publication No. 10-2011-0060356 discloses a cosmetic composition for skin irritation mitigation and anti- And an extract of a rice. Korean Patent Registration No. 10-0823078 discloses a cosmetic composition for anti-inflammation or antioxidation containing an extract of Phragaea as an active ingredient. However, the natural product has a weak effect on skin inflammation and does not show a practical effect on symptoms of skin inflammation caused by various routes.

Accordingly, the present inventors have made efforts to develop a composition which is excellent in the treatment and prevention of skin inflammation and has high safety and thus has no skin side effects. As a result, it has been confirmed that the rice extract is effective in the treatment of skin inflammation.

It is an object of the present invention to provide a composition for the treatment and prevention of inflammatory skin diseases comprising Gnaphalium affine D. Don extract as an active ingredient.

It is another object of the present invention to provide a cosmetic or pharmaceutical composition for the treatment and prevention of inflammatory skin diseases comprising the composition.

In one aspect, the present invention relates to a composition for treating and preventing inflammatory skin diseases, which comprises a rice crumb extract as an active ingredient.

According to the present invention, the Gnaphalium affine D. Don is a biennial plant of two-leafed plants of the Chinese cabbage family, and is widely distributed in East Asia including Korea, Japan, and China. It is used as a medicinal herb, and is called as a seedling (鼠 麴 草). It is made from herbs and rice cake, and roots, leaves, flowers, stems and fruits. Rice worms contain 5% Flavonoid glycosides, 0.05% Essential Oil, 0.58% Unsaponifiable Material, trace amounts of Alkaloid and Sterol, Vitamin B, Carotene, Chlorophyll, Resin Resin), fat, and the like. It is known for its efficacy in the oriental medicine for the treatment of seawater, sputum, asthma, bronchitis, cold body aches, musculoskeletal pain, eczema, , Arthritis, bruises, itching of the skin, non-infectious ulcers and wounds, blood pressure drop, gastric ulcers and the like.

In the present invention, at least one selected from the group consisting of leaves, roots, stems, fruits and flowers including the outbreak of rice cake is extracted and used.

The extract can be obtained by using an extraction method and an extraction solvent known in the art, preferably water, an anhydrous or a lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.) A mixed solvent of alcohol and water, acetone, ethyl acetate, chloroform, hexane or 1,3-butylene glycol as an extraction solvent.

In addition, the rice husk extract may be obtained through a conventional purification process in addition to the extraction method using the above-mentioned extraction solvent. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity) The obtained fractions can also be obtained.

The rice crumb extract of the present invention includes all the extracts, fractions and tablets obtained in each step of extraction, fractionation or purification, their diluted solutions, concentrates or dried products.

In the present invention, the inflammatory skin disease refers to a skin disorder or a skin disorder caused by various pathways such as acne or atopic dermatitis.

As a specific example, the activity of Propionibacterium acnes from the rice crush extract of the present invention was examined. As a result, it was found that the rice cocktail extract had remarkably excellent antimicrobial activity against Propionibacterium acnes, .

(IL-8), which is an inflammatory cytokine depending on the concentration of rice crumb extract, after inducing an inflammatory reaction by treating human monocytic cells with propionibacterium acnes, And Tumor Necrosis Factor-alpha (TNF-alpha), the production of IL-8 and TNF-alpha was inhibited by increasing the concentration of the extract of Duckworm of the present invention, 1 was treated with interleukin-4 (IL-4) to induce eotaxin-1, and the degree of extracellular secretion of eotaxin-1 according to the concentration of the extract was determined. As a result, And the extracellular secretion of eotaxin-1 was inhibited. From these results, it was confirmed that the peel extract of the present invention has an anti-inflammatory effect.

In another specific embodiment, nitric oxide (NO) production by induction of osteoclast cells and nitric oxide reduction by the concentration of the extract of rice crumbs were examined. As a result, Nitrogen production was significantly decreased.

Therefore, the extract of rice crest of the present invention reduces the antimicrobial activity against Propionibacterium acnes , the inhibition of inflammatory cytokine, the suppression of secretion of eotaxin-1 and the production of nitrogen monoxide, thereby reducing the inflammatory skin diseases such as acne and atopy ≪ / RTI >

The composition for treating and preventing inflammatory skin diseases of the present invention can provide a desirable anti-inflammatory effect when it contains an effective amount of rice crumb extract. Herein, the term "effective amount" means an amount capable of alleviating the inflammation of the skin while reducing the activity of causative bacteria of inflammatory skin diseases. Such an effective amount will vary depending on the form in which the composition of the present invention is produced, how the composition is applied to the skin, and the time on the skin. For example, when the composition of the present invention is commercialized as a medicament for dermatological treatment for improvement of skin inflammation, anti-inflammatory ingredients may be contained at a higher concentration than in the case where it is commercialized as cosmetics that are routinely applied to the skin. In the case of a cosmetic product, a wash-off type cosmetic product such as a make-up removing agent, a detergent or the like in which the active ingredient of the present invention stays on the skin in a short period of time may contain a relatively high concentration of anti-inflammatory ingredients . On the other hand, leave-on type cosmetics such as lotion, cream, essence, and the like, in which the active ingredient remains on the skin for a long period of time, may contain lower concentrations of anti-inflammatory ingredients than the wash- will be. In one embodiment of the present invention, the compositions (for example, cosmetic compositions, pharmaceutical compositions) to be used for various purposes may contain 0.0001 to 15% by weight, preferably 0.0001 to 15% by weight, 10% by weight, and more preferably 0.0001% by weight to 5% by weight. When the weight of the extract is less than 0.0001% by weight, the antibacterial and anti-inflammatory effects against acne diseases or atopic dermatitis are insufficient. When the weight of the extract is more than 15% by weight, the effect of increasing the content is very weak . However, as described above, the content is only an aspect of the present invention.

The composition for the treatment and prevention of inflammatory skin diseases of the present invention may further include known anti-inflammatory and / or antibacterial components for the treatment and prevention of the above-mentioned diseases, in addition to the rice crumb extract. However, the additional anti-inflammatory and / or antimicrobial components should not reduce the efficacy and stability of the rice extract, and should be selected in consideration of skin safety, ease of formulation, and the like. The additional anti-inflammatory and / or antimicrobial component may be included in the composition in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the weight% may be selected from the group consisting of anti-inflammatory or antimicrobial activity, skin safety, . ≪ / RTI >

In the present invention, the composition for treating or preventing inflammatory skin diseases may be a pharmaceutical composition or a cosmetic composition. When the composition for treating and preventing inflammatory skin diseases of the present invention is formulated into medicines or cosmetics, it may contain known dermatologically acceptable excipients which act as a carrier for the active ingredient. When formulated as a medicament, the contents disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can be referred to. When formulating into cosmetics, International cosmetic ingredient dictionary, 6th ed., The cosmetic, Toiletry and Fragrance Association , Inc., Washington, 1995, which is incorporated herein by reference in its entirety. Which are incorporated herein by reference.

When the composition for treating or preventing an inflammatory skin disease of the present invention is a cosmetic composition, the composition may include, but is not limited to, an emollient such as a facial lotion, a facial lotion, a body lotion, A makeup remover such as a cream, an essence, a cosmetic cream, a spray, a gel, a pack, a sunscreen, a makeup base, a liquid type, a solid type or a spray type, a powder such as a cream, a cleansing lotion, Detergents such as cleansing foams, soaps, body wash, and the like. In this case, the cosmetic composition may contain a surfactant, an emulsifier, a soap acid, a solvent, a coloring agent, a preservative, an antioxidant, a defoamer, an antibacterial agent, an anticorrosion agent, an enzyme, a plant or mineral oil, a fat, a fluorescent substance, a fungicide, Preservatives, proteins, silicones, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, waxes, and the like.

When the composition for treating or preventing an inflammatory skin disease of the present invention is a pharmaceutical composition, the composition may be in the form of tablets, capsules, powders, granules, suspensions, emulsions, syrups, emulsions, It may be formulated into various forms such as an oil, an oil, a gel, a main tablet, a tincture, a bath, a liniment, a lotion, a patch, a pad, In addition, the composition can be administered orally or parenterally, and is preferably parenterally administered, more preferably applied by a local administration method by application.

Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described in detail below. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Is provided to fully convey the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims.

The composition for treating and preventing inflammatory skin diseases, which comprises the extract of rice crumb extract of the present invention as an active ingredient, has antibacterial and anti-inflammatory effects against acne diseases or atopic dermatitis. And can be safely applied to medicines and cosmetics without cytotoxicity and skin side effects.

Figure 1 shows the antimicrobial activity against Propionibacterium acnes of the rice crumb extract.
FIG. 2 is a graph showing the effect of inhibiting the expression of Eotaxin-1 according to the concentration of rice crumb extract when Eotaxin-1 is induced in human normal fibroblasts.
FIG. 3 is a graph showing the effect of nitric oxide reduction according to the concentration of the rice crumb extract when inducing nitric oxide production in the osteoclast. FIG.

Example 1: Production of rice crumb extract

The leaves, stems, fruits, flowers and roots of the rice cake wormwood were collected and washed thoroughly to remove impurities and impurities completely, shaken at 20 to 35 ° C, and pulverized to a particle size of 1 mm or less. Then, 1 kg of the pulverized rice cake powder was immersed in 10 L of 70% ethanol and ultrasonically extracted for 24 hours. Then, the obtained extract was filtered through a filter paper (Advantes, No. 2) and concentrated under reduced pressure to prepare a rice crumb extract.

Example 2: Propionibacterium acnes of ryegrass extract ( Propionibacterium acnes ) On the antimicrobial activity

In order to activate Propionibacterium acnes ATCC 6919, which is an over-the-skin acne causing acne, BHI broth (Brain-Heart infusion broth: 3.7%) was cultured in an anaerobic incubator at 37 ° C for 48 hours Then, 0.1 ml of each of these culture solutions was plated in a BHI solid medium (Brain-Heart infusion broth; 3.7%; agar 1.5%) and dried.

Then, the rice crumb extract of Example 1 was dropped on a paper disk having a diameter of 10 mm at a concentration of 100 μg / ml, and the plate was placed on the solid medium. The plate was incubated in an anaerobic incubator at 37 ° C. for 3 days to confirm the presence or absence of inhibition zone . Erythromycin was used as a positive control. The results are shown in Fig.

As a result of the experiment, it was confirmed that the rice crush extract inhibited the growth of Propionibacterium acnes .

Example 3: Anti-inflammatory activity of Propionibacterium acnes,

Human mononuclear cells; to 24 in the (Human THP-1 cells monocyitc, Korea Cell Line Bank) containing RPMI-1040 medium without fetal bovine serum-well microplate per well was inoculated so that about 1 × 10 ⁶ cells, 5 % CO ₂ Lt; RTI ID = 0.0 > 37 C < / RTI > for 24 hours. Thereafter, 100 占 퐂 / ml of Propionibacterium acnes, 10 and 100 占 퐂 / ml of rice crumb extract, and then cultured again for 48 hours, the cell culture medium was collected to prepare a sample.

Interleukin-8 (IL-8) and Tumor necrosis factor-alpha (TNF-alpha) system were used for the anti-inflammatory activity of the samples prepared above. (R & D, Minneapolis MN, USA) using an ELISA kit (Genzyme, Mineapolis, MN) with an antibody of interleukin-8 (IL-8) and tumor necrosis factor (TNF- IL-8) and tumor necrosis factor (R & D, Minneapolis MN, TNF-alpha). As a control, mononuclear cells not treated with both Propionibacterium acnes and Rawchug extract were used. The results are shown in Table 1.

Control group Example 1 0 / / ml 10 mu g / ml 100 mu g / ml IL-8 54 100 87 64 TNF-alpha 68 100 92 67

As shown in Table 1 above, the extract of rice crumbs suppresses the production of IL-8 and TNF-alpha induced by Propionibacterium acnes, and the production of IL-8 and TNF-alpha And the concentration-dependent tendency was found to be increased.

Example 4: Extracellular secretion inhibition effect of Echotaxin-1

Human normal fibroblast cells (normal human fibroblast, Ajou Dermatology), the DMEM medium is loaded and such that the inoculated per well of about 2 × 10 ⁵ cells in 24-well micro plate that, at 5% CO ₂ concentration Lt; RTI ID = 0.0 > 37 C < / RTI > for 24 hours. Then, 50 and 50 ng / ml of interleukin-4 (R & D, Minneapolis MN, IL-4), which is known to induce Eotaxin-1, were treated with 10 and 100 ppm of rice crumb extract and cultured for 48 hours. And the degree of secretion of eotaxin-1 was measured using an ELISA kit. The results are shown in Fig.

As a result of the experiment, it was shown that the extract of Echotaxin-1 induced by IL-4 decreased the extract of Echotaxin-1, and the concentration of Echotaxin-1 decreased with increasing concentration of Echinosophora koreanum extract Respectively.

Example 5: Reduction effect of nitric oxide in extract of rice crumb

Mouse macrophages (Raw 264.7 cells, Korean Cell Line Bank) were inoculated into a 24-well microplate containing DMEM medium to a concentration of about 2 × 10 ⁵ cells per well and cultured in a 5% CO ₂ Lt; RTI ID = 0.0 > 37 C < / RTI > for 24 hours. Then, 10 and 100 ppm of rice crumb extract were treated and 200ng / ml of LPS (Lipopolysaccharide), which is known to induce the enzyme iNOS (Nitric oxide synthases) responsible for nitrogen monoxide production, was treated together and cultured for 48 hours. Mixed with the same amount of grease reagent (Griess reagent: 5% (w / v) phosphoric acid, 1% (w / v) sulfanilamide, 0.1% (w / c) N- [1-naphthyl] ethylene diamine dihydrochloride) And then measured at 540 nm. The results are shown in Fig.

The results showed that the production of nitric oxide induced by LPS was decreased by the extracts of R. crus than by increasing the concentration of R. rugosa extract.

Example 6: Test for confirming the safety of human skin of rice crumb extract

6-1. Manufacture of external skin preparation including rice extract

In order to confirm the safety of the extracts of the rice clover extracts on human skin, skin external test agents containing the extracts of clover extracts according to the ingredients and contents of the following Table 2 were prepared and tested for skin safety. First, purified water, glycerin, and butylene glycol were mixed and dissolved at a temperature of about 70 DEG C (water phase part), and the other components were dissolved at about 70 DEG C (oil phase part) except for the three components and trimethanolamine. Thereafter, the oil part was added to the water-based part, and the mixture was firstly emulsified with a homomixer (Tokushu Kika, Japan), followed by final addition of trimethanolamine. Next, the bubbles generated in the mixed solution were removed, and the mixture was cooled to room temperature to prepare an external preparation for skin.

ingredient content (%) Control group Test group 1 Test group 2 Purified water 72 72 72 glycerin 8.0 8.0 8.0 Butylene glycol 4.0 4.0 4.0 Duckworm extract 0 0.1 1.0 Caprylic Capri Triglyceride 8.0 8.0 8.0 Squalane 5.0 5.0 5.0 Cetearyl glucide 1.5 1.5 1.5 Sorbitan stearate 0.4 0.4 0.4 Cetearyl alcohol 1.0 1.0 1.0 Trimethanolamine 0.1 0.1 0.1 Gross weight 100 100 100

6-2. Experiment of cumulative stimulation of skin

The skin external preparation prepared by the method of 6-1 was applied to 30 healthy adults for 9 times and 24 hours cumulative pelletization on the upper forearm every other day to determine whether or not the skin extracts stimulate the skin. As a control group, squalane-based external preparations containing no extracts were used.

The pellet was performed using a Finn chamber (Epitest Ltd, Finland), and 15 占 퐇 of each of the external preparations for skin was dropped into the chamber, followed by applying a patch. The degree of the skin reaction on each occasion was scored using Equation 1 below, and the results are shown in Table 3 below.

[Experimental Equation 1]

The number of tests (9 times) and the number of tests (9 times)

At this time, a score of ± is given as 1, a score of 2 is given as +, and a score of 4 is given as ++, and a safe composition is judged when the average degree of reaction is less than 3.

Test substance Number of subjects who responded (persons) Average
Reactivity 1 week 2 weeks 3 weeks Primary Secondary Third Fourth 5th 6th Seventh 8th car 9th ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ ± / + / ++ Control group 2/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0.18 Test group 1 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0.00 Test group 2 2/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0/0/0 0.18 Subject 30 30 30 30 30 30 30 30 30

As shown in Table 3, the numbers of ±, +, and ++ in the control group were 2, 0, and 0 in the primary pellet, respectively, and no skin reaction occurred after the secondary pellet . In test group 2, the number of persons corresponding to ±, +, and ++ was 2, 0, and 0 in the primary pellet, respectively, and no skin reaction occurred after the secondary pellet. The average reactivity of the control group was 0.18, and the average reactivity of the test groups 1 and 2 was 0.00 and 0.18, respectively, which were all below 3, which was judged as a safe composition.

Production Example 1: Preparation of cosmetic material

1-1. Production of flexible lotion

As shown in the following Table 4, a soft lotion containing an extract of rice crumb musk as an active ingredient was prepared according to a conventional method.

ingredient Content (% by weight) Duckworm extract 0.01 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 ethanol 10.0 Triethanolamine 0.1 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

1-2. Manufacture of nutrition lotion

As shown in the following Table 5, a nutritional lotion containing the rice crumb extract as an active ingredient was prepared by a conventional method.

ingredient Content (% by weight) Duckworm extract 0.01 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 5.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

1-3. Manufacture of nutrition cream

As shown in Table 6 below, a nutritional cream containing the rice crumb extract as an active ingredient was prepared by a conventional method.

ingredient Content (% by weight) Duckworm extract 0.01 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

1-4. Manufacture of massage cream

As shown in Table 7 below, a massaging cream containing the rice crumb extract as an active ingredient was prepared according to a conventional method.

ingredient Content (% by weight) Duckworm extract 0.01 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

1-5. Manufacture of packs

A pack containing the rice crumb extract as an active ingredient was prepared according to a conventional method as shown in Table 8 below.

ingredient Content (% by weight) Duckworm extract 0.01 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 Allantoin 0.1 ethanol 5.0 Nonyl phenyl ether 0.3 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

Preparation Example 2: Preparation of pharmaceutical preparations

2-1. Manufacture of Powder

In order to prepare powders containing the rice crumb extract as an active ingredient, the ingredients shown in Table 9 below were mixed and filled in airtight bags to prepare powders.

ingredient Content (g) Duckworm extract 2 Lactose One

2-2. Manufacture of tablets

In order to prepare tablets containing the rice crumb extract as an active ingredient, tablets were prepared by mixing the ingredients shown in the following Table 10 and then tableting according to a conventional method for producing tablets.

ingredient Content (mg) Duckworm extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

2-3. Preparation of capsules

In order to prepare capsules containing the rice crumb extract as an active ingredient, the following ingredients of Table 11 were mixed and filled in gelatin capsules according to the conventional preparation method of capsules to prepare capsules.

ingredient Content (mg) Duckworm extract 100 Corn starch 100 Lactose 100 Magnesium stearate 2

NC: Negative control
PC: Erythromycin treated at 10 ㎍ / ㎖
Rice wormwood: treatment with 100 ㎍ / ㎖ of rice crush extract

Claims (6)

A cosmetic composition for the improvement and prevention of acne caused by Propionibacterium acnes, which contains Gnaphalium affine D. Don Extract as an active ingredient.
delete The cosmetic composition according to claim 1, wherein the extract is contained in an amount of 0.0001 to 15.0% by weight based on the total weight of the composition.
A pharmaceutical composition for the treatment and prevention of acne caused by Propionibacterium acnes which contains Gnaphalium affine D. Don Extract as an active ingredient.
delete 5. The pharmaceutical composition according to claim 4, wherein the extract is contained in an amount of 0.0001 to 15.0% by weight based on the total weight of the composition.

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