KR100847729B1 - A method of removing bad smell of photosynthetic bacteria culture fluid and a medicine for external use comprising fluid removed bad smell - Google Patents

Abstract

A method for removing bad smell from the culture fluid of photosynthetic bacteria is provided to improve suitability for a medicine for external use, so that the culture fluid of photosynthetic bacteria is useful for inhibiting growth of bacteria, and improving skin beauty. The bad smell of the culture fluid of a photosynthetic bacterium selected from Rhodopseudomonas capsulate, Rhodopseudomonas sphaeroides and Rhodospirillum rubrum is removed by distilling or circulating the culture fluid under reduced pressure. A medicine for external use comprises the deodorized culture fluid of photosynthetic bacteria and is respiratory disease-treating agent, skin disease-treating agent or cosmetics. Further, a concentration of the bacterium is 1x10^4 to 1x10^9 cells/ml.

Description

A method for removing odor of photosynthetic bacteria culture medium and external skin preparation containing photosynthetic bacteria culture medium from which odor has been removed {A METHOD OF REMOVING BAD SMELL OF PHOTOSYNTHETIC BACTERIA CULTURE FLUID AND A MEDICINE FOR EXTERNAL USE COMPRISING FLUID REMOVED BAD SMELL}

(A) to (c) of Figure 1 shows the course of treatment of atopic patients using a mixture of photosynthetic bacteria and herbal preparations prepared in Example 4. Figure 1 (a) is a photograph before using the formulation, Figure 1 (b) is a second week after use, Figure 1 (c) is a photograph of six weeks after use. 1 (d) and (e) shows the progress of treatment of acne patients using the photosynthetic bacteria prepared by Example 4 and the formulations mixed with herbal medicine. Figure 1 (d) is a photograph before using the formulation, Figure 1 (e) is a photograph three weeks after use.

Figure 2 shows the change in the keratinous result as a result of using the photosynthetic bacterial cell culture according to the present invention. Changes in the keratinized areas before (a), 2 weeks after application (b) and 2 months after application (c) are shown.

The present invention relates to the removal of odor, which is a problem that occurs when used as an external preparation for skin of animals, including humans, which is useful for skin beauty and has excellent ability to produce and sterilize antimicrobial substances. (Photo Synthetic Bacteria) In particular, the present invention in the photosynthetic bacterium (Rhodopspiriceae), more preferably in the genus Rhodopseudomonas (Rhodopseudomonas) and Rhodospirillum (Rhodospirillum) or Rhodomicrobium (Rhodomicrobium) genus Occurs when one or two or more bacteria are cultured, and the cells, culture medium or cells and culture medium are used in the treatment of skin diseases, respiratory diseases, etc., in direct contact with the skin, and in cosmetics, such as shampoos, skin softeners, and skin moisturizers. It is about odor removal.

In general, photosynthetic bacteria are important microorganisms that form a group in the natural plant cycle. Photosynthetic bacteria are present at 1,000 to 10,000 individuals / cc in the ocean, soil, rivers, and lakes, and are important for the purification of nature. Among the microorganisms that play a role, they have chlorophyll, and they are microorganisms that can assimilate themselves.

Therefore, photosynthetic bacteria act as primary producers in the natural food chain, become foods such as plankton and become a primary factor in the production of fish, and photosynthetic bacteria are components of various amino acids, vitamins, and pigments. It is abundant and has high value as a biological food.

This high nutritional ability increases the resistance of fish to disease when fish feed on photosynthetic bacteria, enabling the production of fish that is highly adaptable to environmental changes.

In addition, the photosynthetic bacteria absorbs and propagates the harmful substances hydrogen sulfide (H₂S), nitrous acid, nitric acid, CO₂, lower fatty acids and organic acids, which are generated in the growth environment of living organisms, in addition to the physiological function of purifying the growth environment. It contains a large amount of protein, high-quality amino acids, nutrients such as vitamins, nucleic acids and red pigments, and physiologically active substances such as ubiquinone.But photosynthetic bacteria contain about 60% protein and vitamin A. and has been variously used in the cultivation of vitamin B _12, vitamin C, such rarities and to contain large amounts of vitamins, it has a bacterial chlorophyll and carotenoid in red followed by a very high nutritional value nontoxic livestock, fish, crops.

In addition, photosynthetic bacteria have the function of removing contaminants occurring in the growing environment, propagating beneficial microorganisms, and antimicrobial substances such as anti-viruses in the cells. It turns out.

For example, Korean Patent Publication No. 2001-0096494 and Japanese Patent Publication No. H08-325158 can suppress pathogenic bacteria in order to solve problems such as bacterial diarrhea problem and removal of various odorous substances, which are the biggest problems of pig and livestock specification farms. A method of using photosynthetic bacteria as a component of an antagonist microbial agent is described.

In recent years, research has been conducted on the method of using photosynthetic bacteria or extracts thereof having such characteristics as a medicine or cosmetics.

For example, Japanese Patent Publication No. 2002-138043 describes photosynthetic bacteria as containing various active ingredients and is useful for the treatment of atopy and athlete's foot, and Japanese Patent Publication No. 2003-055240 as a medicine for restoring health. It is said to be useful for various diseases. In addition, Korean Patent Publication No. 2003-0036187 describes a method for producing an antioxidant by culturing photosynthetic bacteria and describes that the sulfate agent can be used in medicine or cosmetics.

The present inventor has also applied for the use of photosynthetic bacteria in cosmetics and medicine. By the way, the present inventors have found for the first time that the photosynthetic bacterial cell itself or its culture solution is used as a cosmetic or medicament very badly odorous. It is known in the art that photosynthetic bacteria are used to remove wastewater or odors of concentrated products, but when the photosynthetic bacteria are used in the human body, the problem of odor generated by photosynthetic bacteria itself is the first inventor discovered.

When used as a cosmetic or medicament is mostly applied to the skin or spray, but if there is a bad smell, even if the effect is excellent, it will be difficult to use as a cosmetic or medicament.

Accordingly, the present invention is to include the step of removing the odor before the addition, when the photosynthetic bacteria itself or its culture to the cosmetic or medicament. In addition, cosmetics made by extracting specific components of microorganisms are currently used, but there are cases in which living microorganisms are difficult to use due to storage or microbial survival problems when used in medicine or cosmetics. .

Furthermore, the present invention provides a novel method of removing odor from photosynthetic bacteria or culture medium thereof. If photosynthetic bacteria are exposed to air and left to some extent, the odor is eliminated to some extent, but photosynthetic bacteria, which are anaerobic microorganisms, are sensitive to air and temperature and light, and their growth is also affected by these factors. Accordingly, the present inventors have found that if the culture medium is decompressed while circulating the culture solution (also called an umbrella fountain, a waterfall water principle) while maintaining anaerobicity to some extent, photosynthetic bacteria are maintained in a healthy state and odors are removed.

The microbial fields generally used are classified into microbial strains themselves and culture media generated during the metabolic process of microorganisms, and the metabolites are known to have a lot of antimicrobial antiviral effects. Therefore, in the method of extracting the active substance, the microorganisms and the whole culture medium, which are not certain components, are used for cosmetic or medical treatment with this distillate using a form called distillation method.

According to an embodiment of the present invention, in the method of adding the cells of the photosynthetic bacteria, the culture medium or the culture solution containing the cells as an active ingredient to the external skin preparation, the deodorizing step before adding the active ingredient to the external skin preparation Features are provided.

According to another embodiment of the present invention, in the external preparation for skin containing the cells of the photosynthetic bacteria, the culture medium or the culture medium containing the cells as an active ingredient, the external preparation for skin is characterized in that the active ingredient is deodorized.

In the above two embodiments, the photosynthetic bacterium is at least one selected from the group consisting of Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides, and Rhodospirillum rubrum. This is preferred. The external skin preparation is a respiratory disease treatment, a skin disease treatment, or a cosmetic. The bacterium is at a concentration of 1 × 10 ⁴ cells / ml to 1 × 10 ⁹ cells / ml.

In addition, the deodorization is preferably deodorized by distillation or decompression by distilling the active ingredient. In the case of deodorization by distillation, a commercial stock solution containing the cells or a filtered culture solution may be generally used on the market. Distillation has generally been called distillation to increase the purity of alcohol. In addition, distillate is a distilled herb that has been known in recent years. Decoction of Chinese medicine by distilling the primary extract is a form of water, such as water is taken to the patient. These distilled herbs are controversial in terms of their effectiveness and ingredient extraction methods, but the distilled herbs used in Hamaia and Oriental Medicine (currently active Korean medicine chain stores) that use distilled herbs have been proven effective and extract the efficacy of specific herbs. I can prove that it exists. Until now, the traditional Chinese medicine extract method has been completely different from the conventional Chinese medicine extract in the visual and taste aspects, but it has a similar effect on the human body.

When the culture solution containing the photosynthetic bacteria according to the present invention was distilled and used as an external preparation for skin, the odor of the culture solution was greatly reduced. In addition, the deodorization by distillation, while the cosmetic and pharmacological effect of the culture of the photosynthetic bacteria is somewhat reduced, there is another advantage that the risk of preserving living cells and contamination risk is considerably reduced. In fact, as a result of using the distillate according to the present invention as an external preparation for skin, its cosmetic function and pharmacological effect tended to decrease somewhat, but its preservation period and odor removal effect were very large. Since the distillate of photosynthetic bacteria distilled in this way has excellent sulfate action, it can be added to medicine, food, cosmetics, etc. as a sulfate agent.

In addition, the deodorizing is preferably deodorizing while circulating a culture solution containing the cells. In this case, a circulation means for culturing fluid is provided at the bottom of a completely sealed or partially sealed culture tank, and a vacuum means such as a fan and a compressor is provided at the top, and the culture medium is circulated under reduced pressure while circulating the culture with the circulation means. Removed.

In the case of decompression of the culture medium containing the cells, if the degree of decompression is too high, the cells are killed so that a negative pressure of 5-70 mmHg is applied at atmospheric pressure. If the degree of decompression is too severe, the cells are killed, which is not preferable when using live cells.

Further, the addition of deodorant such as charcoal, bamboo charcoal in the culture solution increases the effect of deodorization. It is also possible to maximize the deodorizing effect by floating the green tea leaves on the top.

In this way, the odor is removed and the anaerobic state is maintained. When the microorganism and the culture medium continue to circulate in the tank, about 90% of the odor disappears after 72 hours, and even when used as an external preparation for skin, the odor is removed. As the decompression time passes, the deodorizing effect becomes better. In order to continue to use as an external preparation for skin, the culture medium once deodorized for about 72 hours increases odor due to the growth of the cells. In this case, it is preferable to depressurize periodically for 6 hours every day.

A concrete example will be described with reference to FIG. 3. 1) A ventilator 6 is installed on the upper part of the somewhat sealed tank 8 made of an impermeable material to suck the air in the tank to the outside while maintaining the anaerobic condition (pressure reduction method). The microbial culture solution in the tank is circulated up and down by discharging the culture medium at the bottom through the tube 3 using the umbrella fountain 2. The lower portion of the tank 8 may be additionally provided with an outlet having a precipitate filtration function to extract photosynthetic bacteria and culture medium as necessary. The upper portion of the tank is provided with a closed lid 10 to enable the injection of the culture solution, and also put a deodorant such as charcoal, bamboo charcoal, etc. in the crepe supported from the top by the rope 12 to increase the deodorizing effect.

The active ingredient according to the present invention, that is, a photosynthetic bacterium or a culture solution containing the same, is immediately deodorized and mixed with other ingredients or used as an external preparation for skin.

The present invention relates to cosmetics and their use of animals, including humans using deodorized photosynthetic bacteria, and particularly the present invention is a red-yellow sulfur bacteria (Rhodopspiriceae family), more preferred among photosynthetic bacteria. Preferably, one or two or more bacteria are cultured in the genus Rhodopseudomonas, Rhodospirillum, or Rhodomicrobium, and the preparation containing the culture medium or cells and the culture medium is prepared. It can be used as a cosmetic, skin disease treatment agent and respiratory disease treatment agent in direct contact with shampoo, skin softener, skin moisturizer, scalp care agent, herbal medicine and the like.

Skin diseases include atopy, acne, viral skin diseases, bad breath, stomatitis, sulphitis, liquid odor, esophagitis, women's uterine diseases (uterine infection, cold sores, etc.), cystosis (囊 濕 症) ), Hair loss, eye disease, and skin mucosal diseases. In addition, it can be used not only for humans but also for diseases of pets, and is particularly useful for pet hair loss.

Respiratory diseases include, for example, allergic rhinitis, hypertrophic rhinitis, atrophic rhinitis, sinusitis disease, cold disease.

Skin external preparations, such as skin disease treatments or cosmetics according to the present invention, by directly using the culture medium or cells and the culture solution in the form of a liquid or spray to the affected area, or by mixing a thickener or ocher such as xanthan gum to make a gel or gel state immediately Can be applied to or used as a pack. Alternatively, it may be mixed with herbal medicines such as herbal extracts, distilled herbs, herbal cosmetics, and the like to achieve the effects of herbal treatments and combination treatments for various skin diseases and skin aesthetics.

Thickeners include xanthan gum, carbomer, gelatin, triethanol amine and the like. In addition to the thickening agent, you may contain together Gemer and methyl paraffin as a preservative. Moreover, you may contain a softening agent.

The cause of atopy is mainly due to abnormal immune system and environmental factors. When the antigen acts on the human body, free radicals are released from the phagocytes of the human body and the phagocytosis is fed by phagocytosis.However, when exposed to many antigens due to environmental causes, immune function is lost or the balance is broken. Attacking tissue causes atopy or various skin diseases. Among photosynthetic microorganisms, red sulfur-free bacterium has the highest nitrogen fixation ability and affects the circulation of carbon, nitrogen, and hydrogen on the earth, and is one of the strongest microorganisms on earth to fix nitrogen. The microorganisms are also fed with unsaturated fatty acids to cultivate hydrogen sulfide and lower fatty acids.

Looking specifically at the cause and treatment mechanism of atopy, active oxygen and unsaturated fatty acids are generated by the antigen suppression action of the body's skin immune system against antigens, both of which forms nitrogen compounds such as nitrogen peroxide. Such active oxygen, unsaturated fatty acids, nitrogen compounds, etc. may also cause skin diseases such as atopy, acne, photosynthetic bacteria according to the present invention to reproduce them as a nutrient source to remove the causative agent of skin diseases to induce a therapeutic effect Seems to be.

In addition, atopy and psoriasis are symptoms caused by the accumulation of pathogenic microorganisms and skin waste due to environmental pollution and skin breakdown of the immune system, photosynthetic bacteria according to the present invention decompose hydrogen sulfide, ammonia, toxic amine, nitrite and It cures skin wastes with its ability to inhibit and fundamentally treat atopy and psoriasis.

Acne develops into purulent acne due to inflammation caused by the action of hair follicles and propionibacterium acne in the pores and chemical reactions in the pores in oily skin, that is, the sebum-rich body constitution. (Sebum) Decompose ability and the food chain of Demodex worms first occupy the inhibition of the growth of Demodex worms, so the effect of erosion can be treated acne.

Photosynthetic bacteria generally refer to bacteria that grow by using light as an energy source, and are classified into red sulfur bacteria, red sulfur-free bacteria, and red green sulfur bacteria. In the present invention, red sulfur-free bacteria are preferred. Among them, microorganisms belonging to the genus Rhodopspirillum, Rhodopseudomonas, and Rhodomicrobium belong to Rhopspirillaceae (Rhopspirillaceae). Among them, rubrum, tenue, fulvum, molischianum, and photometricum are among the microorganisms belonging to the genus Rhodopspirillum, and acidophila, capsulata, gelationsa, palustris, sphaeroides, viridis, and vannielii species in the genus Rhodopseudomonas.

Among them, Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides, and Rhodospirillum rubrum are particularly preferred. Among them, Rhodopseudomonas sphaeroides is more preferable.

Photosynthetic bacteria according to the present invention tends to have a higher therapeutic effect than those exhibiting antiviral or antimicrobial properties.

In addition, the culture of the bacteria is more effective than the photosynthetic bacteria itself, and containing the bacteria and the culture solution is more effective and there is no separate process of removing the bacteria, it is also easy to manufacture. Furthermore, the separation of specific substances from cells or cultures would be effective for skin diseases and respiratory diseases. The superior antimicrobial component in photosynthetic microbial culture was identified as D-Glucitol, 1,4-isosorbide dinitrate, and the chemical formula is C ₆ H ₈ N ₂ O ₈ Was estimated.

The culture of these bacteria or the bacteria themselves may be used as a single species or a mixture of two or more species.

The bacterium according to the present invention may be distributed from a depositing institution or separated by a separation method known in nature. For example, Rhodopseudomonas capsulata is deposited in ATCC 33303, Rhodopseudomonas sphaeroides in accession no. KCTC-8849P, Rhodospirillum rubrum is deposited in IFO 3986. Can be distributed from each depository institution.

Explains the use of skin diseases or cosmetics. Although not limited thereto, the cell concentration may be diluted with purified water to a culture solution of 1 × 10 ⁴ cells / ml to 1 × 10 ⁹ cells / ml, preferably 1 × 10 ⁶ cells / ml to 1 × 10 ⁸ cells / ml. It is preferable to use.

Furthermore, in the present invention, in addition to the photosynthetic bacteria, yeast, lactic acid bacteria, Bacillus microorganisms and the like may be further contained in a concentration similar to the photosynthetic bacteria in a concentration similar to the photosynthetic microorganisms. These yeasts or lactic acid bacteria, Bacillus microorganisms may be harmless to the human body.

For the treatment of skin diseases, the herbal distillate may contain 0.2 to 1 times and other thickener or softener materials 2 to 6 times for the cells, cultures, and extract preparations thereof according to the present invention.

Also, in nasal diseases (rhinitis cold sinusitis, etc.), 10 ~ 30% of the cell culture medium is sprayed and sprayed with 5 ~ 10% of herbal medicine, 5 ~ 10% of saline solution, 50 ~ 80% of distilled water, and 0.1% of menthol component. good.

Women's vaginitis may be sprayed or distilled water or other liquid containing 10% photosynthetic microorganisms and culture medium, or may be used as a cleaning agent.

In scalp disease (alopecia, dry skin keratinization, dandruff, etc.), photosynthetic bacteria were mixed with water at about 1 ~ 10% and washed with water.

Natural herbal extract in the present invention is a process of extracting the common common pharmaceutical ingredients by mixing an appropriate amount of baekbaekpi (桑白皮), licorice (甘草), silk powder (method of mixing a certain amount of herbal medicine and water 3-4 hours), Alternatively, a method of extracting an herbal medicine with an extract preparation, or a method of mixing with microorganisms by powdering and concentrating with a distilled herbal medicine may be adopted. For example, 600g of 600g each of lettuce, licorice, and silk powder is put in 10,000 cc of water. Generally, it takes about 3-4 hours to extract about 5000 ~ 6000cc of the medicine. . In this extract, the photosynthetic microorganism and the culture medium may be mixed and used at an appropriate ratio, and the component may be extracted in a distilled medicine.

Generally, the ratio of Chinese medicine is 5 ~ 10% with thickener, and the liquid spray type is similar.

The above-mentioned medicinal herb ingredient is umbeliferone, scopoletin, flavonoid [flavonoid (morusin, mulberrin)], mulberrochromene cyclomulberin, cyclo water The pharmacological action of chloroclome (cyclomulberrochromene) has the healing and prevention of dry skin, skin whitening, skin moisturizing action.

Licorice has triterpene saponin, glycyrrhizin, uralene, and flavonoids, which neutralize skin whitening and toxic effects. Silk powder is composed of 6.34% of ash, 67.44% of protein, 4.38% of fat, 11.31% of moisture, and it is known to have a skin whitening effect.

In addition, the mixing ratio of baekryepi, licorice, silk powder of the above-described natural herbal medicines were mixed by arbitrarily adjusting in the ratio range of 1-5: 1-5: 1-5 with respect to each weight.

The daily dosage of the formulation according to the invention is 1 cm ² based on the liquid formulation at a cell concentration of 1 × 10 ⁷ / ml. About 0.05ml-0.7 ml per sugar is suitable, it can be divided and administered, and can be used several times a day for effective treatment. Since photosynthetic bacteria according to the present invention are harmless to the human body, the amount of use of the photosynthetic bacteria may be high, but the cost is high.

In the present invention, when prepared in herbal packs (deodorized photosynthetic bacterial culture, herbal distillate and thickener), it is preferable to use once a day, and in severe cases, when the microorganisms are well adapted to their skin, the frequency of use may be increased. For example, when the rash occurs, you can rest for 2-3 days and start again. You can ignore the light rash and continue to use it. Simultaneous use with other drugs is also acceptable.

As a result of clinical application to date, the photosynthetic bacterial preparation according to the present invention could not feel any special side effects.

Herbal packs can decompose other components due to increased microbial activity when not refrigerated. Low temperature on storage is required. Herbal packs should be refrigerated and the lid should be closed to prevent infection by other hospital microorganisms.

In the case of the use of bathing agents, daily use is preferable because there is a possibility of invading other pathogens. When bathing, the temperature is preferably 26-39 ° C., because the bacterium has the strongest activity at the temperature of 26-38 ° C.

Bathing agents are good for systemic diseases, and if you want partial treatment, you can use a pack or mix photosynthetic bacteria and culture with an appropriate amount of water. The preparation itself is a bathing agent, and may be mixed with a decoction of the herbal medicine. Herbal packs should be mixed with Chinese medicine.

According to another embodiment of the present invention, when a 1-30 mL portion of the photosynthetic bacterial culture is mixed with a cosmetic, such as 100 mL of cosmetics (including color cosmetics) having a water-soluble lotion, skin, liquid or gel properties, Atopic dermatitis caused by viral infections, acne, and other skin diseases can be cured and prevented, as well as skin whitening, moisturizing, suppleness can be obtained.

 According to another embodiment of the present invention, when a 1-30 mL portion of the photosynthetic bacteria culture medium is mixed with a scalp care agent having a liquid or gel form such as shampoo, hair dye, rinse, and water soap 100 ml, hair loss and dandruff or As well as healing and preventing skin infections of the scalp caused by viral infections and viruses, the skin whitening, moisturizing and softening effect can be obtained.

100 ml of the deodorized photosynthetic bacteria of the present invention may be mixed with 50-500 L of bath water and applied as a bath.

As described above, it may be used by mixing with the existing cosmetics, or, when producing a cosmetic product, may be added to the deodorized photosynthetic bacteria culture medium according to the present invention.

For example, in the case of preparing a hair rinse combination shampoo, 30 g of KOH and 20 g of NaOH are dissolved in 180 g of water, and mixed with 40 g of olive melt of about 70 ° C. and 200 g of palm oil, saponified for 2 hours, and then slowly cooled. To this, a solution of 5 g of fragrance dissolved in 150 g of alcohol at 50 ° C. is added while stirring, and the solution is left until it becomes pale yellow. To 360 g of pure water, a mixed solution of 100 ml of the photosynthetic bacterial culture drug according to the present invention was added to the above saponification solution, matured, and then cooled and filtered to prepare a hair rinse combined shampoo.

In the preparation of cosmetics, the cosmetics according to the present invention are various in the whole composition, under the conditions in which the photosynthetic bacteria, the culture medium or the extract thereof is added in an amount of 1 to 30 parts by weight at a cell concentration of 1 × 10 ⁸ / ml. Known additives may be used.

The additive may be, for example, a useful ingredient and a water-soluble ingredient that are effective in forming a formulation and protecting the skin, which are widely used in skin protection and skin treatment products. Representative useful ingredients include hydrocarbons such as paraffin oil, squalane, squalene, and the like. ; Synthetic triglycerides obtained by reacting with a natural product such as capric capric triglyceride; Ster oil for the synthesis of cetyl octanoate, octyl dodecanol, isopropyl palmitate; Vegetable oils such as jojoba oil, olive oil, soybean oil, moon soybean oil, sancho oil, sesame oil and perilla oil; Animal oils such as mink oil; Fat-soluble vitamins such as vitamins A, E, and F, and derivatives thereof, and the like; as the water-soluble component, polyols such as propylene glycol, butylene glycol, glycerin, and polyethylene glycol; Mucopolysaccharides such as hyaluronic acid, chondroitin sulfate, and glucosamine; Water-soluble vitamins such as vitamin C and panthenol and derivatives thereof; Benzophenone derivative ultraviolet absorbers including homomenthyl salicylate benzophenone, 2-hydroxy benzophenone, 4-methoxy benzophenone; Cinnamic acid derivatives such as ethylhexyl-p-methoxycinnamate and octylmethoxycinnamate; Ultraviolet absorbers such as butylmethoxydibenzoylmethane, albutin, gojiksan, and bilberry extracts, which have skin whitening effects, and alpha hydroxy acids, pancreses, and allantoin and amino acids, which promote skin circulation. Additives such as protein, flavonoids, milk, honey and extracts such as Angelica, Cheongung, Ginseng, Bellflower, Marigold, Green Tea and Wild Chrysanthemum, which are effective for skin protection, and dihydroxyacetone with skin pigmentation And zinc oxide, which has a soothing effect on the skin. In addition, as an ingredient of the skin protection agent and skin treatment composition of the present invention, an active ingredient used for the treatment of the skin may be used. For example, salicylic acid and alpha hydride that promote the circulation of keratin in skin bleach hydroquinone and acne treatment products. Loxy acid, sulfur, an enzyme pancrease, hydrocortisone component of the adrenal cortex hormone agent for treating skin diseases may be used. The above-listed ingredients are ingredients that can be used in the present invention without particular limitations, and thus, the use of ingredients that can be used in skin protectants and skin treatments other than these is not limited. However, in the oil-soluble emulsifier according to the present invention, since the activity is suspended and the possibility of death, an aqueous emulsifier capable of growing the cells according to the present invention is preferable.

The cosmetic composition according to the present invention can be divided into a cream formulation without fluidity at room temperature and a lotion formulation with fluidity at room temperature, and these formulations can be used as various kinds of skin protection and skin treatment cosmetics according to the purpose of the added active ingredient. have. For example, moisturizing creams and lotions, night creams, eye creams, cleansing creams and lotions, sunscreen creams and lotions, sun tanning creams and lotions, whitening creams and lotions, wrinkle improvement creams and lotions, acne improvement creams and lotions, atopy skin Basic cosmetics such as improvement creams and lotions, dispersion cosmetics such as foundations, makeup bases, hair cosmetics such as hair dyes, hair treatment creams, hair creams, skin bleaching products of emulsified cream and lotion formulations in external medicines, and wrinkle treatment It can be used in emulsified creams and lotion skin care products such as products, acne treatment products, adrenal cortex hormone products.

Next, the case where the photosynthetic bacterium of the present invention is used for the treatment of respiratory diseases will be described. It is more effective to contain herbs or other ingredients that are useful for respiratory diseases. When used in combination with herbal medicine and the like, one or more distilled herbal liquids selected from the group consisting of Changji, Shinji, white paper, and peppermint extract in the entire formulation may contain 1 to 30.0% by volume.

And in the group consisting of physiological saline (or sodium chloride appropriate amount), menthol (menthol) and peppermint (pepermint) at least one of 0.03-7% by volume, preferably 0.05-5% by volume.

For example, Changchang, Shingi, white paper and peppermint 600g each and 10,000cc of water in a medicine bath, which is commonly used in oriental medicine, about 3-4 hours to extract about 5,000cc. This extract is extracted again with a distillation bath used for distillation and extracts about 3,000 cc. This extract is used by mixing photosynthetic microorganisms and culture medium at an appropriate ratio. Usually, the amount of herbal medicine is about 2-5%. 2-5 ml of the distilled solution, 15-23 ml of purified water having a concentration of photosynthetic microorganisms in the range of 1 × 10 ⁷ cells / ml to 1 × 10 ⁹ cells / ml, and menthol 0.05-0.2 ml, peppermint 0.05-0.2 ml, sodium chloride Mix 0.1 ~ 10mg to make 30cc and use it in a spray type bottle.

In other words, 6-15% by volume of Chinese herbal distilled extracts such as Changi, Shinyi, white paper, and peppermint, photosynthetic microorganisms (can further include microorganisms of the Bacillus, yeast, etc.) 40-75% by volume of purified water, 7-30% by normal saline And 0.1% by volume of peppermint and the like.

For use example, 10 ^eight photosynthetic microorganism of cell / ml concentration 5-10cc, 10 ⁸ by making the degree of 30cc to the Bacillus microorganism 0.5-5 cc, 18cc of distilled water, physiological saline 2cc containing peppermint 0.1 cc of the liquid cell / ml concentration It was.

The daily dosage of the preparation according to the present invention is appropriate 0.05ml-0.7ml per 1 cm ² of skin, may be administered in divided doses.

The preparations according to the present invention can be prepared in various forms known in the art, but it is preferable to prepare the liquid so that it can be sprayed directly into the nasal cavity.

Changjaza (Xanthium strumarium) of the used herbal medicine is composed of xanthostrumarin (xanthostrumarin), fatty oil, alkaloids, vitamin C, etc., to treat sinusitis, nasal congestion, respiratory discomfort, odor and runny nose Effective for symptoms

The preparation containing the photosynthetic bacterial culture medium or the cells according to the present invention as an active ingredient is excellent in increasing and improving the therapeutic effect of allergic rhinitis, hypertrophic rhinitis, atrophic rhinitis, sinusitis disease, and use as a nasal cleaning agent. It is also useful for the treatment of other respiratory diseases, including cold diseases.

The formulations according to the invention are applicable to all animals, including humans.

In addition, the preparation containing the photosynthetic bacteria culture medium or the cells according to the present invention as an active ingredient is further enhanced when the other herbal ingredients known to be effective in treating nasal diseases.

As a Chinese herbal medicine, Maginolia liliflora is composed mainly of volatile essential oils, flavonoids and alkaloids, and its action is astringent to rhinitis, protecting the mucosal surface and expanding capillaries and improving local blood circulation. Improves to relieve inflammation, improve breathing and relieve symptoms. In addition, it has the inhibitory action of skin fungi and staphylococci and increases the treatment rate for rhinitis, sinusitis, and irritable rhinitis.

Angelica dahurica is composed of viaak-angelica, oxypeucedanin, viak-angelicol, imperatonin, and isoimeratortorin. It is composed of pepopterin, which is effective in the treatment of headaches caused by external symptoms, stuffy nose, runny nose and sinusitis.

As described above, distilled herbal medicine by distillation herbal bath is beneficial for the treatment of nasal disease because it eliminates the characteristic of Chinese medicine and the smell of herbal medicine sensitive to nose.

All of these natural herbal medicines may be used together, or any one or more thereof may be used. The relative amounts of the extracts were extracted and distilled with a water bath at the ratio of Changja: Shini: White paper: Mint = 1 ~ 2: 1 ~ 2: 1 ~ 2: 1 ~ 2.

Photosynthetic bacteria can be cultured by a generally known method. For example, the culture medium may be a medium containing K ₂ HPO _{4 ,} NaCl, MgSO ₄ · 7H ₂ O, Na-Acetate, yeast extract, and peptone as main components.

In addition, when the culture medium is made using a concentrated liquid medium in which the medium of photosynthetic bacteria is concentrated 20 to 50 times, the nutrients and the medium are chelated in preparation of the concentrate to remove the inconvenience of mixing and concentrating the microelements separately. By concentrating together, it is also possible to use it conveniently when culturing photosynthetic bacteria using a concentrated medium.

In addition, when making concentrated liquid medium of photosynthetic bacteria, EDTA should be dissolved in water first, followed by mixing and dissolving nutrients and trace elements in order to prevent precipitation.

The optimal medium for culturing the photosynthetic bacteria first dissolved NH ₄ Cl, NaHCO _{3 ,} K ₂ HPO _{4 ,} KH ₂ PO _{4 ,} NaCl, yeast extract, Biotin, p-Aminobenzoic acid, and chelate EDTA-2Na, a substance, was dissolved in 5 to 20 parts by weight of the concentrated liquid medium, mixed, and then CaCl ₂ · 2H ₂ O, MgSO ₄ · 7H ₂ O were added, and trace inorganic elements FeCl ₃ · 6H ₂ O, CuSO ₄ · 5H ₂ O, H ₃ BO ₃ MnCl ₃ · 4H ₂ O, ZnSO ₄ · 7H ₂ O, Co (NO ₃ ) ₂ · 6H ₂ O It is convenient to use 10 to 50 times concentrated medium of bacteria.

Medium conditions and culture conditions are preferably managed to be excellent in the antimicrobial or antiviral properties of photosynthetic bacteria.

For example, specific medium compositions and culture methods are as follows.

(Photosynthetic bacterial culture medium composition and culture)

Composition of the optimum medium for culturing photosynthetic bacteria in the present invention is as follows.

For 1,000 ml of H₂O, 1 ~ 5g of NHHCl, NaHCO₃, K₂HPO₂, KH₂PO 효, NaCl, Yeast extract, Biotin, and ｐ-Aminobenzoic acid (PABA) are mixed and dissolved first to obtain an inorganic concentrate, and 100% by weight of the obtained inorganic concentrate 5 to 20 parts by weight of the chelating material EDTA-2Na and one of the trace inorganic elements CaCl₂ · 2H2O, MgSO₄ · 7H₂O, FeCl₃ · 6H₂O, CuSO₄ · 5H₂O, H₃BO₃MnCl₃ · 4H₂O, ZnSO₄ · 7H₂O, Co (NO₃) ₂ · 6H₂O Selecting the above, melt about 0.6g on the basis of the total weight, mix and concentrate to obtain the optimum medium for photosynthetic bacteria without precipitation.

The medium thus obtained was autoclaved and placed in a sterile culture bottle, one or two selected from red sulfur-free bacteria belonging to the genus Rhodopseudomonas and Rhodospirillum or Rhodomicrobium among photosynthetic bacteria. Inoculate more than one species of bacteria, fill with nitrogen gas (N ₂ gas) to make anaerobic, and incubate for 3 days under 25 ~ 35 ℃ temperature and 2,000 ~ 10,000 Lux light conditions. 1 × 10 cells / ml (n = 8)] or cultured under the above conditions for 7 days, the cells were collected by centrifugation, and then 100,000,000 cells per ml [1 × 10 cells / ml] were collected in distilled water. (n = 8)].

Referring to the embodiment for obtaining the cultures thus obtained are as follows. In Examples, Example 5 and Test Example 2 are the test of the effect of the culture medium after the deodorization step, and the rest of the examples are examples of the external skin preparation using the photosynthetic bacteria not subjected to the deodorization step.

Example 1

The culture medium composition of the invention was K₂HPO₄0.5g, NaCl 0.1g, MgSO₄7H2O 0.2g, Na- Acetate 3g, Yeast extract 3g, Pepton 3g and H₂O 1000ml and trace inorganic elements CaCl₂ · 2H ₂ O 0.5 g, FeCl₃ · 6H₂O 0.025mg, CuSO₄ · 5H₂O 0.00025mg, H₃BO₃ 0.005mg, MnCl₃ · 4H₂O 0.0003mg, ZnSO₄ · 7H₂O 0.005mg, Co (NO₃) ₂ · 6H₂O 0.0025mg, dissolved, mixed, concentrated and autoclaved Rhodopseudomonas capsulata (ATCC33303), Rhodopseudomonas sphaeroides (accession no. After inoculating 100 ml of the seed solution of Rhodopspillum rubrum (IFO 3986), each was filled with nitrogen gas and sealed to make it anaerobic, and then incubated for 3 days under 2,000 lux light conditions at 30 ° C. About 100,000,000 cell cultures It was.

Example 2

Under the same conditions as in Example 1, inoculated with 100 ml of the seed solution of Rhodoshudomonas capellata, Rhodoshudomonas spheroids, Rhodospirilum rubron belonging to the red no sulfur bacteria among photosynthetic bacteria, and incubated for 7 days The cells were collected by centrifugation and diluted in distilled water to 100,000,000 [1 × 10 ⁿ cells / ml (n = 8)] per 1 ml of the cells.

Among the photosynthetic bacteria used in Examples 1 and 2 above, Rhodoshudomonas capellata, Rhodoshudomonas spheroids, and Rhodospirilum rubrun belonging to the red no sulfur bacterium are antimicrobial generating ability as shown in <Table 1>. The effect of inhibiting the growth of and pathogens is excellent, and among them, Rhodopseudomonas sphaeroides, the accession number KCTC-8849P mainly used in the present invention, it can be seen that the excellent effect (Table 1 See <Antibacterial test of photosynthetic bacteria>.

Note 1) 8849P strain is Rhodopseudomonas sphaeroides KCTC-8849P

     33303 strain is Rhodopseudomonas capsulata (ATCC33303)

     3986 strain is Rhodopspillum rubrum (IFO 3986)

Note 2) + growth inhibition, ± slight growth inhibition,-growth

Examples in which the cells of the photosynthetic bacteria cultured according to Example 1 or the culture solution containing the same are applied to hair and scalp treatments, respiratory disease treatments, cosmetics, herbal cosmetics, bathing agents, shampoos, rinses, and the like. .

Example 3

In Example 3 of the present invention, the photosynthetic red sulfur-free bacterial culture medium of Rhodoshudomonas capsule lata, Rhodoshudomonas spheroids, and Rhodospirilum rubrene obtained in Example 1 was purified to have a cell count of about 10,000,000 cells per ml. After fold dilution, the mixture was mixed at the same ratio to prepare a liquid formulation.

1 cm ^{2 of} skin per day for atopic, acne and other viral skin diseases 0.3 ml of sugar was applied to the skin or sprayed. In Table 2, the therapeutic effect using only three species of red sulfur-free bacteria cultured according to the present invention is shown in Application Example 1.

Example 4

Thickener consisting of 20% by weight of photosynthetic bacterial culture medium having carbomer, triethanol amine, and gemll having a number of cells of about 100,000,000 cells per ml of culture medium in which three kinds of cell culture medium obtained in Example 1 were mixed at the same ratio. (Ultrasound Transmission Gel ^TM , Sungwon) 75% by weight and 5% extract of lettuce, licorice and silk powder were prepared in a gel to prepare atopy, acne, and other viral skin diseases. About 1 cm ² skin per day on the skin 0.3 g of sugar was applied as in the following to examine the course of treatment. The results are shown in <Table 2> and <Table 3>.

Test Example 1

Unlike Examples 3 and 4, the same method as Example 4 was used for atopic dermatitis, acne, and other viral skin diseases without mixing the photosynthetic bacteria cultured as in the present invention and using pure herbal skin alone (Comparative Example 1). As a result of applying directly to the skin, the results were obtained as shown in Table 2 (treatment rate when the combination of microorganisms and natural herbal medicines).

As shown in Table 2, 45% of the healing effect (45 out of 100 patients had sufficient healing effect as a result of using photosynthetic bacteria cultured as in the present invention against atopy, acne and other viral skin diseases). And a combination of the photosynthetic bacteria culture medium and the herbal preparation of the present invention resulted in a high healing effect of 65%, and was used as a commercially available herbal preparation (Comparative Example 1). Only about% can obtain a healing effect, when the photosynthetic bacteria alone or a mixture of herbal medicine of the present invention was obtained, a two to three times higher healing effect was obtained compared to general herbal medicine treatment.

<Table 3> shows the treatment results for 63 atopic patients, acne patients 33, and 16 other viral skin diseases, the formulation according to Example 4 mixed with the photosynthetic bacteria culture medium of the present invention mixed with herbal medicines (Example of mixing photosynthetic bacteria and herbal medicine of the present invention)

As a result, in the case of atopy, more than 50% of the healers reached 88.3%, the cure rate of 80% or more reached 38%, 63.5% of the sick showed more than 70% healing, and in the case of acne, more than 50% of the healers totaled 91.0 %, The cure rate over 80% reaches 27.3%, 51.5% of the patients have a healing effect of 70% or more, and almost 100% of the other viral skin diseases have a healing effect of more than 50%, 80 The complete cure rate of more than 3% reached 31.5%, and 75% of the patients had a healing effect of more than 70%. (Mean healing period 2-6 months).

(A) to (c) of Figure 1 shows the course of treatment of atopic patients using a mixture of photosynthetic bacteria and herbal preparations prepared in Example 4. This patient is a 28-year-old woman, Figure 1 (a) is a photograph before using the formulation, Figure 1 (b) is a second week after use, Figure 1 (c) is a photograph six weeks after use. It can be seen that the atopic symptoms of the woman almost disappeared after use of the preparation according to Example 4.

1 (d) and (e) shows the progress of treatment of acne patients using the photosynthetic bacteria prepared by Example 4 and the formulations mixed with herbal medicine. This patient is a 22-year-old woman, Figure 1 (d) is a photograph before using the formulation, Figure 1 (e) is a photograph three weeks after use. Acne symptoms of the woman after the use of the formulation according to Example 4 can be seen that almost disappeared after 3 weeks of use.

Example 5

Distillate obtained by distilling the culture medium containing the cells in 25% by weight (Example 5-1) having a cell count of about 100,000,000 cells per 1 ml of the culture medium prepared in Example 4, using a distillate of Daewoong Electric Co., Ltd. for 24 hours. 25 weight% (Example 5-2), 25 weight% of cell culture medium (Example 5-3) deodorized the culture medium with the apparatus shown in FIG. 3, and 0.05 weight% of vitamin B ₁₂ (Comparative Example 2) were effective. A skin cosmetic was prepared by adding it as a component. <Skin cosmetic composition (unit: weight%)>

Example 5-1 Example 5-2 Example 5-3 Comparative Example 2 Culture solution (not deodorized) 25 - - - Distillate of culture - 25 - - Culture solution (deodorization) - - 25 - Vitamin B ₁₂ - - - 0.05

Test Example 2

In order to measure the effect of the cosmetics obtained in Example 5 and Comparative Example 2, 30 women in their 20s and 50s were divided into three groups, and the use of the product was evaluated for three months. The evaluation was conducted on the items such as odor, whitening effect, smoothness when using the product, skin shine, etc., divided into very good, good, normal, and poor, and the results are shown in Table 6. As can be seen from Table 6, Example 5 of the present invention showed a superior effect than Comparative Example 2. <Example 5-1, 5-2, 5-3 and Experimental results of use of the skin cosmetic of Comparative Example 2>

Item / Product Example 5-1 Example 5-2 Example 5-3 Comparative Example 2 Whitening effect ○ ○ ○○ △ Smoothness ○○ ○○ ○○ ○ Skin shine ○○ ○ ○○ △ smell × ○○ ○ ○○               ○○: Very good, ○: Good, △: Normal, ×: Poor

Example 6

The composition of the optimum medium for culturing photosynthetic bacteria is K ₂ HPO ₄ 0 _. 5 g, NaCl 0.1 g, MgSO ₄ · 7H ₂ O 0.2 g, Na-Acetate 3 g, Hydration extract 3 g, Peptone 3 g and H ₂ O 1 L to be sterilized in a sterile culture bottle, Rhodoshudomonas capsulerata (Rhodopseudomonas capsulata) (ATCC33303), Rhodopseudomonas sphaeroides (Accession No. KCTC-8849P), and Rhodospirillum rubrum (IFO 3986) spawn solution (1 × 10 ⁸ cells / ml), each was inoculated with N ₂ gas to make anaerobic and then incubated at 30 ° C. under 1000 Lux light conditions until the number of cells became 1 × 10 ⁸ cells / ml. The cells were harvested by centrifugation.

Example 7

The cells collected in Example 6 were diluted to 1 × 10 ⁸ cells / ml in distilled water, respectively, and then Rhodopseudomonas capsulata (ATCC33303) A preparation, Rhodopseudomonas sphaeroides (deposited). No. KCTC-8849P) B formulation, Rhodospirillum rubrum (IFO 3986) C formulation, and Formulation D, in which the three strains were mixed in the same ratio, were obtained.

Example 8

Changji, Shinji, white paper and peppermint 600g each was mixed with 10,000ml of water and 5,000ml of decoction solution was extracted again with a distillation bath using 4ml from 3,000ml. 4 ml of the distilled liquor, 21 ml of the photosynthetic bacterial preparation obtained in Example 7, and 0.1 ml of menthol, 0.1 ml of peppermint, and 4.9 ml of physiological saline were mixed to prepare Formulations A-1, B-1, C-1, and D-1 were obtained.

Example 9

In the same manner as in Example 6, three photosynthetic microorganisms were cultured to have a cell concentration of 1 × 10 ⁸ cells / ml, and then, a solution containing three strains containing the same strain was mixed in the same state without separating the cells from the culture medium. Obtained. Formulation D-2 was obtained by adding a herbal distillate, menthol, and the like to 21 ml of the obtained culture in the same manner as in Example 8.

Test Example 3

Non-inflammatory patients in three clinics using the formulations A, B, C and D prepared in Examples 6 to 8 and A-1, B-1, C-1 and D-1, and D-2 The healing effect of rhinitis was observed.

Each formulation was sprayed 2 ml per day for allergic rhinitis patients to observe the results. The number of patients per each clinic was 45 to 84. Allergic rhinitis was difficult to determine whether X-Ray improved.

As shown in Table 5 below, about 40% of patients with rhinitis have at least some symptoms of improvement, indicating that the photosynthetic microorganisms have a high therapeutic effect.

In addition, it can be seen that the healing effects of Formulations A-1, B-1, C-1 and D-1 in combination with distillation herbs are more excellent. In addition, the preparation containing the culture medium with the cells was more effective than the cells contained in the purified water.

In this test example, the effect began to appear one month from the day of administration, and at 3 months, most patients had improved symptoms.

Formulation Rep. 1 Rep. 2 Member of 3 Improvement (%) Some improvement No response Improvement Some improvement No response Improvement Some improvement No response A 17 18 65 14 19 67 14 15 71 B 19 23 58 20 22 58 17 23 60 C 8 10 82 5 12 83 7 7 86 D 24 25 51 21 27 52 23 25 52 A-1 21 24 55 21 22 57 28 18 54 B-1 25 26 49 24 28 48 21 27 52 C-1 11 17 72 12 13 75 11 10 79 D-1 26 27 47 25 29 46 25 30 45 D-2 28 34 38 28 32 40 27 32 41

(Improvement: 2 or more of the symptoms of runny nose, nasal congestion, sneezing are reduced by 50% or more.

Test Example 4

Using the photosynthetic bacteria culture medium prepared in the same manner as in Example 3, 20ml was sprayed onto the vagina of 21 women with uterine disease 2 to 3 times per day, and then the treatment progress was observed.

Two weeks after the start of treatment, seven patients had symptomatic improvement (reduced pruritus, decreased cold), and ten patients at one month and 14 patients at three months.

In general, when antibiotics are used, relapses are often recurred after symptom improvement, whereas the relapse rate of the preparations according to the present invention is also low.

Test Example 5

Using the photosynthetic bacterial cell cultures prepared in the same manner as in Example 3, 15 ml was sprayed with a head spray of 20 patients having alopecia three times a day, and then the treatment progress was observed.

One month after the start of treatment, 12 patients had reduced hair loss by more than 30%. Three months after the start of treatment, 12 patients had a reduction in symptoms of more than 50%.

Test Example 6 Improvement of Skin Exfoliation

Using a photosynthetic bacterial cell culture solution prepared in the same manner as in Example 3 was sprayed on the skin of 20 people with skin keratin phenomena 10 to 2 times 3 times per day to examine the progress of skin keratinization improvement.

Two weeks after the start of the application, the area of keratin was reduced by more than 50% in 16 people, and after 8 weeks, the area of keratin was decreased in most people, and 15 patients improved to the extent that there was almost no keratin. For example, Figure 2 shows a change in the corneous portion of a 9-year-old man before application (a), 2 weeks after application (b), 2 months after application (c), 2 months after application ( In Figure 2 (c) it can be seen that most of the keratin portion was reduced.

Test Example 7 Reduction of Dandruff

      Using the photosynthetic bacterial cell culture solution prepared in the same manner as in Example 3, shampoo after spraying the hair of 20 people with dandruff by 20ml 2 to 3 times per day to watch the progress of dandruff reduction.

To measure the effects of dandruff reduction, 20 women in their 20s and 50s were used for 3 months to evaluate the effects of dandruff reduction. As a result, five people had a 80% -100% reduction in dandruff, eight had a 60% -80% reduction, three had a 40% -60% reduction, and four had a 0% -20% reduction compared to before use. As a result, there was a reduction of dandruff in most people.

Reference Example 1

Rp. Was identified by Rhodopseudomonas sphaeroides (Accession No. KCTC-8849P) and known separation methods from soil. For the gelatinosa NA75 strain, the antimicrobial activity of E. coli bacteria and supernatants was investigated. Two microorganisms were cultured and 4L of culture was centrifuged to separate the cells into supernatant and the antimicrobial activity was detected by agar medium diffusion. Both strains showed stronger antimicrobial activity than supernatants. (Antimicrobial activity of cells and supernatant)

  strains              clear zone on plate (10 mg / disk) Rhodopseudomonas sphaeroides Rp . gelatinosa NA75    supernatant cellbody   supernatant    cellbody  E. coli 17 ^a)   4 16 4

a) diameter (mm)

Cell culture or photosynthetic bacteria culture medium according to the present invention has an effect that the odor is removed when used as a skin external agent such as respiratory disease treatment, skin disease treatment, cosmetics through a deodorizing step, greatly increasing the suitability as a skin external preparation.

Claims (12)

A method of adding a culture medium obtained by culturing photosynthetic bacteria to an external skin preparation as an active ingredient, wherein the active ingredient is subjected to a deodorizing step prior to addition to the external skin preparation.  The method according to claim 1, wherein the photosynthetic bacteria is Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides (Rhodopseudomonas sphaeroides), and Rhodospirillum rubrum (Rhodospirillum rubrum) is characterized in that any one or more selected from the group consisting of How to. The method of claim 1, wherein the external skin preparation is a respiratory disease treatment, a skin disease treatment, or a cosmetic. The method of claim 1, wherein the bacterium has a concentration of 1 × 10 ⁴ cells / ml to 1 × 10 ⁹ cells / ml. The method according to any one of claims 1 to 4, wherein the deodorization step is characterized in that the active ingredient is distilled to deodorize. The method according to any one of claims 1 to 4, wherein the deodorizing step is to deodorize the active ingredient under reduced pressure. The method of claim 6, wherein the deodorizing step is characterized in that the deodorizing while circulating the culture. An external preparation for skin containing a culture medium obtained by culturing photosynthetic bacteria as an active ingredient, wherein the active ingredient is deodorized.  The method according to claim 8, wherein the photosynthetic bacteria is one or more species selected from the group consisting of Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides, and Rhodospirillum rubrum Skin external preparation to do. The skin external preparation according to claim 8, wherein the external skin preparation is a respiratory disease treatment, a skin disease treatment, or a cosmetic. The skin external preparation according to any one of claims 8 to 10, wherein the deodorization is obtained by distilling the active ingredient to deodorize it. The skin external preparation according to any one of claims 8 to 10, wherein the deodorization is deodorized under reduced pressure while circulating the active ingredient.

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