KR20120001161A - Composition for moisturizing skin containing extracts prepared by enzyme process - Google Patents

Abstract

PURPOSE: A composition containing bamboo leaf or green tea extract is provided to promote transglutaminase-1 synthesis and skin moisturizing. CONSTITUTION: A composition for skin moisturizing contains 0.001-10.0 wt% of one or more extract as an active ingredient, which is prepared by enzyme treatment of bamboo leaves or green tea. The composition further contains 0.001-10.0 wt% of extract of soybeans, Nymphaea alba, or Opuntia Ficus-indica var. saboten. The used enzyme is amylase, protease, glycosidase, lactase, sucrose, or maltase. The composition promotes the synthesis of transglutaminase-1.

Description

Composition for moisturizing skin containing extracts prepared by enzyme process}

The present invention relates to a skin moisturizing composition comprising an extract obtained by enzymatic treatment.

Decreased skin moisture often causes dead skin cells to dry out. In addition, the gloss of the skin disappears, fine lines are formed easily, and skin aging begins early. That is, in order to have improved skin, it is essential to maintain skin moisture, and for this purpose, various efficacy ingredients having skin moisturizing effects are used.

On the other hand, in the case of extracting natural products to obtain the efficacy components, the components that can be obtained according to the extraction method may vary, there are many cases in which the conventional solvent extraction method can not extract a variety of components having a variety of types and efficacy.

The present invention is to provide a composition having a skin moisturizing effect by including one or more extracts of bamboo leaves and green tea obtained by the enzyme treatment.

 One aspect of the invention provides a skin moisturizing composition comprising as an active ingredient one or more extracts of bamboo leaves and green tea obtained by enzymatically treating one or more of the bamboo leaves and green tea.

Another aspect of the present invention provides a composition for moisturizing the skin further comprising at least one extract selected from the group consisting of soybean, water lily and white lotus in the composition.

Another aspect of the invention provides a cosmetic composition comprising the composition.

The composition according to one aspect of the present invention comprises one or more extracts of bamboo leaves and green tea obtained by enzymatically treating one or more of the bamboo leaves and green tea, thereby synthesizing transglutaminase-1. It has excellent skin barrier function and skin moisturizing effect. The composition according to another aspect of the present invention is an enzyme treatment of one or more of the bamboo leaves and green tea, but at least one extract of the bamboo leaves and green tea obtained by the high pressure treatment before, after the enzyme treatment or enzyme treatment as an active ingredient By including it, it is further promoted Transglutaminase-1 synthesis has a better skin barrier function and skin moisturizing effect. The composition according to another aspect of the present invention further comprises one or more extracts selected from the group consisting of soybeans, water lilies and white lotuses, thereby exhibiting synergistic effects and having excellent skin barrier function and skin moisturizing effect.

1 is a graph showing the change (%) of transdermal moisture loss (TEWL) with time after applying the composition according to the present invention on the skin.
Figure 2 is a graph showing the skin moisture content over time after applying the composition according to the invention on the skin.

As used herein, the term "extract" includes all materials irrespective of the amount and type of components, as long as the substance is obtained by extracting components therefrom from natural products. For example, it is a broad concept that includes all components that are dissolved or decomposed from natural products using a solvent or enzyme, those obtained by extracting only specific components such as oils, and certain components of other natural products.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is a broad concept including not only tissues covering the body surface such as the face or body, but also the scalp and hair.

Hereinafter, the present invention will be described in detail.

One aspect of the present invention provides a skin moisturizing composition comprising, as an active ingredient, one or more extracts of bamboo leaves and green tea obtained by enzymatically treating one or more of bamboo leaves and green tea. Another aspect of the present invention is an enzyme treatment of one or more of the bamboo leaves and green tea, one or more extracts of the bamboo leaves and green tea obtained by treating at a pressure of 400 to 800 MPa before, during or after the enzyme treatment It provides a skin moisturizing composition comprising as an active ingredient.

Skin moisturizing composition according to an aspect of the present invention comprises one or more extracts of bamboo leaves and green tea obtained by the enzymatic treatment of one or more of the bamboo leaves and green tea as an active ingredient. As described above, the method of obtaining an extract by treating the enzyme may prepare an extract including various types and a large amount of potent ingredients by treating the enzyme, unlike other general extraction methods. In another aspect of the present invention, the bamboo leaf and green tea extract may be obtained by mixing the extract obtained by enzymatically treating the bamboo leaf and green tea, or may be obtained by enzymatically treating the bamboo leaf and green tea together.

Skin moisturizing composition according to another aspect of the present invention is treated with one or more of bamboo leaves and green tea, but before the enzyme treatment, after the enzyme treatment or enzyme treatment at a high pressure, for example, a pressure of 400 to 800 MPa, At least one extract of decomposed, obtained bamboo leaves and green tea is included as an active ingredient. Thus, when the high-pressure treatment in addition to the enzyme treatment, it is possible to produce an extract containing a wider variety of types and a large amount of potent components as compared to the case of the enzyme treatment as well as other general extraction methods. Among them, in the case of enzymatic treatment at high pressure, an extract containing the most various kinds and a large amount of potency components can be prepared.

In particular, one or more extracts of bamboo leaves and green tea obtained by enzymatic treatment and optionally high pressure treatment contain large amounts of components that promote the synthesis of Transglutaminase-1. Since transglutaminase-1 is an essential element for the formation and maintenance of the stratum corneum, promoting the synthesis of transglutaminase-1 may enhance skin barrier function and protect skin moisture. Thus, extracts of one or more of bamboo leaves and green tea obtained by enzymatic treatment and optionally high pressure treatment may further enhance skin barrier function and have a better skin moisturizing effect.

The method of obtaining the extract by enzymatic treatment of the plants is described in more detail as follows:

In one aspect of the invention, a method of enzymatically treating a plant to obtain an extract comprises enzymatically treating the plant to decompose it.

In one aspect of the invention, the enzyme is one or more selected from the group consisting of amylase, protease, glycosidase, lactase, lactase, sucrose and maltase Include.

In one aspect of the invention, the enzyme treatment temperature may be adjusted according to the active temperature range of the enzyme used. In another aspect of the invention, the enzyme treatment temperature may be 30 to 60 ℃. In another aspect of the invention, the enzyme treatment temperature may be 35 to 40 ℃. If the enzyme treatment temperature is less than 30 ° C., the enzyme is difficult to activate and if it exceeds 60 ° C., most of the enzymes are destroyed and lose their function, so the temperature in the above range may be preferable.

In one aspect of the invention, the weight ratio of plant and enzyme may be 100,000: 1 to 100: 1. If the weight ratio of the plant and enzyme is less than 100,000: 1, the amount of enzyme is too small to extract the effective ingredient, and if it exceeds 100: 1, the increase in the effective ingredient of the extract is inefficient compared to the amount of enzyme added.

In one aspect of the invention, the method of enzymatically treating a plant to obtain an extract may further comprise the step of autoclaving the plant before the enzymatically treating the plant to decompose. In another aspect of the present invention, the method of enzymatically treating a plant to obtain an extract may further comprise the step of subjecting the plant to high pressure after enzymatically treating the plant. In the method of enzymatically treating a plant according to another aspect of the present invention to obtain an extract, the step of enzymatically treating the plant may be enzymatically treating the plant at a high pressure, and this method may be most preferred.

In one aspect of the invention, the high pressure may mean 400 to 1000MPa. In another aspect of the invention, the high pressure may mean 400 to 800MPa. In another aspect of the invention, high pressure may mean 600 MPa, which is similar to undersea pressure of 6,000 m depth. At pressures below 400 MPa, the potency components are not sufficiently extracted, and at 800 MPa pressures, the potency component extraction increase is insignificant compared to the high pressure, and thus inefficient.

In one aspect of the present invention, the method of enzymatically treating a plant to obtain an extract may further comprise filtering the degraded solution after enzymatically treating the plant.

In one aspect of the present invention, a plant extract from which impurities are removed can be obtained by filtering a solution decomposed by enzymatic treatment. In another aspect of the present invention, the filtration may use any method commonly used in the art, and specifically, may use filtration through a fine filter paper. Through this filtration, impurities can be removed to obtain an extract of the purified plant.

In one aspect of the present invention, the method of enzymatically treating a plant to obtain an extract may further comprise diluting the filtered solution after filtering the degraded solution.

In one aspect of the invention, the filtered plant extract may be diluted in a suitable solvent for ease of use. In another aspect of the present invention, the solvent includes all solvents commonly used in the art, and specifically includes water, butylene glycol or a mixed solvent thereof.

In one aspect of the present invention, the method of enzymatically treating the plant to obtain an extract is applicable to all plants to prepare the extract by a general extraction method. In another aspect of the present invention, the plant includes one or more selected from the group consisting of bamboo leaves, green tea, beans, water lilies and white lotus flowers. In another aspect of the invention, the plant comprises one or more of bamboo leaves and green tea.

One aspect of the present invention provides a skin moisturizing composition comprising, as an active ingredient, one or more extracts of bamboo leaves and green tea obtained by high pressure treatment of one or more of the bamboo leaves and green tea. In another aspect of the invention, the high pressure may mean 400 to 1000MPa. In another aspect of the invention, the high pressure may mean 400 to 800MPa. In another aspect of the invention, the high pressure may mean 600MPa.

One aspect of the present invention provides a skin moisturizing composition comprising one or more extracts of bamboo leaves and green tea obtained by extracting and enzymatically treating one or more of bamboo leaves and green tea as an active ingredient, for example, solvent extraction. do. Another aspect of the present invention is a skin moisturizing agent comprising one or more extracts of bamboo leaves and green tea obtained by high pressure, for example, 400 to 800 MPa pressure treatment before, after enzyme treatment or during enzyme treatment as an active ingredient. It provides a composition for.

In this specification, general extraction methods include all conventional extraction methods in the art. Specifically, it may be a solvent extraction method in which each plant is leached in water or an organic solvent, and purified and separated. The organic solvent is not particularly limited and may be C ₁ to C ₅ lower alcohol, ether, ethyl acetate or chloroform. The lower alcohol of C ₁ ~ C ₅ may be, for example, any one or two or more mixed solvents selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol. .

Skin moisturizing composition according to an aspect of the present invention may further include one or more extracts selected from the group consisting of soybean, water lily and white lotus. One or more extracts selected from the group consisting of soybeans, water lilies and white lotuses also have skin moisturizing effects alone, and when included together with one or more extracts of bamboo leaves and green tea obtained by the enzyme treatment, synergistic effects are exerted. Skin barrier function and skin moisturizing effect can be exhibited.

In the above, the bamboo leaf refers to the leaves of bamboo ( Bambusoideae ) generically referred to as an evergreen perennial plant belonging to a monocotyledonous rice plant (rice) and bamboo subfamily. Green tea is not fermented ( Camellia sinensis ) leaf. Beans ( Glycine max ) is a perennial plant of the dicotyledonous rosewood legume, Nymphaea teragona ) is a generic term for the dicotyledonous plant, Buttercup and Water Lily plants. In one aspect of the invention the soybean, water lily or white lotus ( Opuntia Coccinellifera ) comprises one or more of each and every part. For example, the beans, water lilies, or white lotus flowers may include one or more of each leaf, flower, stem, root, fruit, and seed.

In the skin moisturizing composition according to an aspect of the present invention, one or more extracts of bamboo leaves and green tea may each be included in an amount of 0.001 to 10.0% by weight based on the total weight of the total composition. In the skin moisturizing composition according to another aspect of the present invention, one or more extracts of bamboo leaves and green tea may each be included in an amount of 0.1 to 5.0 wt% based on the total weight of the total composition.

In the skin moisturizing composition according to an aspect of the present invention, one or more extracts selected from the group consisting of soybeans, water lilies and white lotuses may be included in an amount of 0.001 to 10.0% by weight, based on the total weight of the total composition. In the skin moisturizing composition according to another aspect of the present invention, one or more extracts selected from the group consisting of soybeans, water lilies and white lotuses may be included in an amount of 0.1 to 5.0 wt% based on the total weight of the total composition.

When used in the above range, it is not only suitable for showing the intended effect of the present invention, but also satisfies both the stability and safety of the extract, and may be appropriate to use in the above range in terms of cost-effectiveness. More specifically, when the extract contains less than 0.001% by weight, the skin moisturizing effect by the extract is insignificant, and when it contains more than 10% by weight, the effect increase rate may be low and inefficient.

One aspect of the present invention provides a cosmetic composition comprising a composition comprising one or more extracts of bamboo leaves and green tea obtained by enzyme treatment and optionally one or more extracts selected from the group consisting of green tea, soybean, water lily and white lotus to provide.

The cosmetic composition may be, for example, a cosmetic composition, the cosmetic appearance of which includes a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, e.g. emulsions obtained by dispersing the oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases, emulsions obtained by dispersing the aqueous phase in oil phases, multiemulsions, suspensions, micro emulsions, micro It may be provided in the form of an aerosol composition containing capsules, microgranulocytes, ionic (liposomal) and nonionic vesicle dispersants, foams or compressed propellants. These compositions may be prepared according to conventional methods in the art.

Cosmetic compositions may include fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers , Metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or auxiliaries commonly used in the cosmetic or dermatological fields. have. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological fields.

The cosmetic composition is not particularly limited in formulation, and may be appropriately selected in accordance with the desired purpose. For example, flexible lotion, astringent lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing lotion, cleansing foam, cleansing water, pack, powder, conceal stick, body lotion, body Creams, body oils, body essences, body cleansers, hair dyes, hair tonics, hair nourishing lotions, hair essences, hair serums, scalp treatments, hair treatments, hair conditioners, hair shampoos, hair lotions, toothpastes, mouthwashes, hair conditioners , Wool, lotions, ointments, gels, creams, patches, and sprays may be prepared in one or more formulations selected from the group consisting of, but not limited to.

Hereinafter, the structure and effects of the present invention will be described in more detail with reference to Preparation Examples, Examples, Comparative Examples and Test Examples. However, these preparation examples, examples, comparative examples and test examples are provided only for the purpose of illustration to help the understanding of the present invention is not limited to the scope and scope of the present invention.

[Production Examples 1 and 2]

At 600 MPa, 30-40 ° C., bamboo leaves and green tea were decomposed by mixing with amylase, respectively. The enzymatically digested solution was filtered through a fine filter paper to remove impurities. The filtered extract was diluted with a mixed solvent of water and butylene glycol to prepare Preparation Examples 1 and 2, respectively.

Test Example 1 Evaluation of Transglutaminase-1 Synthesis Promoting Effect

In order to measure the skin moisturizing effect, the bamboo leaf extract of the above preparation, bamboo leaf extract extracted using a general solvent (ethanol) extraction method, and transglutaminase-1 synthesis of calcium chloride which is a representative transglutaminase-1 synthesis promoting component The promoting effect was compared.

First, a human skin cell line (Cascade Biologics) was placed in a 96-hole flat plate incubator with 5 × 10 ⁴ cells per cell and attached for 24 hours. After the test material was treated to the attached skin cell line, and after 2 days, the medium was removed and stored in a -20 ° C refrigerator. Freeze-thawing was repeated twice to destroy cells treated with substances, and then treated with acetone: ethanol (1: 1, v / v) stored at -20 ° C and left at 4 ° C for 30 minutes. The cells were fixed. After standing at room temperature, the organic solvent was evaporated, blocking (1% bovine serum albumin), transglutaminase antibody (primary antibody), HRP anti-mouse antibody (secondary) was carried out, OPD (o-phenyldiamine) ) Was added. The amount of expression was calculated by measuring the absorbance at 490 nm, and the calibration was performed by measuring the background at 630 nm. Transglutaminase-1 synthesis promotion rate was calculated by comparison with the absorbance value of the untreated control group, and the results are shown in Table 1 below.

division Concentration (mg / ml) Promoting Synthesis of Transglutaminase-1 (%) High Pressure Enzyme Treated Bamboo Leaf Extract (Manufacturing Example 1) 12.5 137.0 ± 1.9 50 161.0 ± 4.0 Ethanol Extract Bamboo Leaf Extract 12.5 no effect 50 no effect Calcium chloride 1.5mM 169.9 ± 6.4

As shown in Table 1, it was confirmed that the bamboo leaf extract obtained by the enzyme treatment at high pressure of the preparation example has a transglutaminase-1 synthesis promoting effect unlike the bamboo leaf extract extracted by the ethanol extraction method. In addition, when the bamboo leaf extract of the preparation example is used at 50mg / ml, it was confirmed that there is about 95% transglutaminase-1 synthesis promoting effect compared to 1.5 mM of calcium chloride, which is a representative transglutaminase-1 synthesis promoting component. .

[Comparative Examples 1 and 2 and Examples 1 to 6]

To prepare Comparative Examples 1 and 2 and Examples 1 to 6 including the composition of Table 2 below.

Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Bamboo leaf extract One - - - - - - - Green tea extract - One - - - - - - High Pressure Enzyme Treated Bamboo Leaf Extract (Manufacturing Example 1) - - One - - - - One High Pressure Enzyme Treated Green Tea Extract (Manufacturing Example 2) - - - One - - - One Bean extract - - - - One - - One White lotus extract - - - - - One - One Water lily extract - - - - - - One One

In Table 2, the bamboo leaf extract, green tea extract, soybean extract, white lotus extract and water lily extract are extracts extracted by a general solvent extraction method, and the high-pressure enzyme-treated bamboo leaves and green tea extracts are extracts obtained by enzymatic treatment at high pressure of the preparation. Concentrations of all extracts were used equally at 1% by weight based on the total weight of the composition.

Test Example 2 TWEL Evaluation

Twenty adult men and women were coated with the compositions of Comparative Examples 1 and 2 and Examples 1 to 6 on the skin and measured TWEL (Transepidermal Water Loss) values for 48 hours. When the TWEL value was 100 before application of the composition, the TWEL value was evaluated after 6 hours, 24 hours, and 48 hours, and is shown in FIG. 1.

As can be seen in Figure 1, when applied to Example 1 or 2 TWEL value was lower than when applying Comparative Example 1 or 2, when Example 6 was applied, the TWEL value was the lowest. Therefore, the TEWL value of the extract obtained by enzymatic treatment at high pressure is lower than the extract by general solvent extraction method, and the composition comprising all of the bamboo leaf and green tea extract, soybean extract, white lotus extract and water lily extract obtained by enzymatic treatment at high pressure. The lowest TEWL value is the most effective in preventing skin moisture loss.

[Comparative Examples 3 to 5 and Examples 7 to 12]

To the cosmetic composition of Comparative Examples 3 to 5 and Examples 7 to 12 with the composition of Table 3 below. The specific manufacturing method is as follows. First, the oil phase component of Table 3 was heated at 70 to 75 ℃ and mixed uniformly. Separately, the aqueous phase component 1 in Table 3 was heated at 70-75 ° C., dissolved and mixed uniformly. The emulsion was prepared by adding an oil phase component while stirring the aqueous phase component 1 while maintaining a temperature of 70 to 75 ° C. Then, the alcohol component was added and mixed, and the aqueous phase component 2 was continuously added, and then cooled to 30 ° C.

division Raw material name Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Comparative Example 3 Comparative Example 4 Comparative Example 5 Oily ingredient Pentaerythritol tetraethylhexanoate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Vegetable squalane 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Cyclomethicone 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 PCM methicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Polysorbate 20 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Cyclopentasiloxane / dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Water component 1 Bamboo sap To100 To100 To100 To100 To100 To100 To100 To100 To100 Disodium ID 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Hydrogenated Dressin / C12-16 Alcohol / Palmetic Acid 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Dimethicone Copolyol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Butylene Glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Propanediol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Fiji / Fiji-17 / 6 copolymer 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Methylgluses-20 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Tromethamine 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Betaine 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Bamboo extract - - - - - - 1.0 - - Green tea extract - - - - - - - 1.0 - Autoclaved Bamboo Leaf Extract 1.0 1.0 - - - - - - - Autoclaved Green Tea Extract 1.0 - 1.0 - - - - - - Bean extract 1.0 - - 1.0 - - - - - White lotus extract 1.0 - - - 1.0 - - - - Water lily extract 1.0 - - - - 1.0 - - - Alcohol ingredients ethanol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 antiseptic 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 incense 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Water component 2 Carbomer 1% Bamboo Sap 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0

[Test Example 3] Skin moisture content change

Twenty adult men and women who complained of dryness or dry skin were selected and the cosmetic compositions of Examples 7 to 12 and Comparative Examples 3 to 5 were applied to the forearms after waiting for 1 hour in a constant temperature and humidity condition. Skin moisture content was measured before, immediately after, and after 4, 8 and 24 hours of application and the results are shown in FIG. 2.

As can be seen in Figure 2, when applying the Example 7, it was confirmed that there is almost no change in the skin moisture content when comparing the application immediately after the application for 24 hours. On the other hand, Comparative Example 3 was confirmed that the skin moisture content after 24 hours is significantly reduced compared to immediately after application. That is, the moisturizing effect of the cosmetic composition comprising at least one extract of bamboo leaves and green tea obtained by the high-pressure enzyme treatment is better than the extract by the general solvent extraction method, which is further improved when the soybean extract, white lotus extract and water lily extract are included. can confirm.

Examples of the formulation of the composition according to one aspect of the present invention will be described below, but can be applied to other various formulations, which are intended to explain in detail only and not intended to limit the present invention.

[Formulation Example 1] Lotion

ingredient weight% Example 6 5.0 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Purified water Balance

Formulation Example 2 Cream

Compounding ingredient Content (% by weight) Example 6 1.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / Capric Triglycerides 3.0 Squalane 5.0 Cetearyl Glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Quantity Purified water Balance

Formulation Example 3 Ointment

ingredient weight% Caprine / Capryl Triglycerides 10 Liquid paraffin 10 Solbitan Sesquioleate 6 Octyldodeceth-25 9 3,7 hexanoate 10 Squalane One Salicylic acid One glycerin 15 Sorbitol One Example 6 2 Preservative, coloring, fragrance Quantity Purified water Balance

Claims (10)

Skin moisturizing composition comprising, as an active ingredient, at least one extract of bamboo leaves and green tea obtained by enzymatically treating one or more of bamboo leaves and green tea. The method of claim 1,
At least one extract of bamboo leaves and green tea is a composition for moisturizing skin, characterized in that obtained by treating at least one of bamboo leaves and green tea at a pressure of 400 to 800 MPa before, during or after enzyme treatment. The method according to claim 1 or 2,
The composition is a composition for moisturizing the skin further comprising at least one extract selected from the group consisting of soybean, water lily and white lotus. The method according to claim 1 or 2,
The enzyme is moisturizing skin, characterized in that at least one selected from the group consisting of amylase (protease), glycosidase (glycosidase), lactase (lactase), sucrose (sucrose) and maltase (maltase) Composition. The method according to claim 1 or 2,
Enzyme treatment temperature is a skin moisturizing composition, characterized in that 30 to 60 ℃. The method according to claim 1 or 2,
At least one of bamboo leaves and green tea and the weight ratio of the enzyme is 100,000 to 1, respectively, characterized in that the skin moisturizing composition. The method according to claim 1 or 2,
One or more extracts of bamboo leaves and green tea are each moisturizing composition, characterized in that it comprises 0.001 to 10.0% by weight based on the total weight of the total composition. The method of claim 3, wherein
At least one extract selected from the group consisting of soybeans, water lilies and white lotuses is moisturizing composition, characterized in that it comprises 0.001 to 10.0% by weight, based on the total weight of the total composition. The method according to claim 1 or 2,
The composition is characterized in that to promote the transglutaminase-1 (Transglutaminase-1) synthesis. Cosmetic composition comprising a composition according to claim 1.

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