KR101914162B1 - Composition for moisturizing skin containing extracts prepared by enzyme process - Google Patents

Abstract

The present invention discloses a skin moisturizing composition comprising, as an active ingredient, an extract of at least one of bamboo leaves and green tea obtained by enzymatically treating at least one of bamboo leaves and green tea. The present invention also discloses a cosmetic composition comprising the composition. The composition promotes the synthesis of Transglutaminase-1 and exhibits excellent skin moisturizing effect.

Description

[0001] The present invention relates to a composition for skin moisturizing comprising extracts obtained by enzymatic treatment.

The present invention relates to a skin moisturizing composition comprising an extract obtained by an enzyme treatment.

Reduced skin moisture often results in dry keratin. Also, the skin's luster disappears, fine wrinkles easily occur, and skin aging begins early. That is, in order to have improved skin, it is essential to maintain skin moisture. To this end, various effective ingredients showing skin moisturizing effect have been used.

On the other hand, when extracting natural products to obtain effective ingredients, the components obtainable according to the extraction method may be different, and in many cases, various components having various kinds and efficacy can not be extracted by the conventional solvent extraction method.

Korean Patent No. 10-0881143 (January 22, 2009)

The present invention provides a composition having skin moisturizing effect by containing an extract of at least one of bamboo leaves and green tea obtained by an enzyme treatment.

One aspect of the invention provides a skin moisturizing composition comprising, as an active ingredient, an extract of at least one of bamboo leaves and green tea obtained by enzymatic treatment of at least one of bamboo leaves and green tea.

Another aspect of the present invention provides a skin moisturizing composition, which further comprises at least one extract selected from the group consisting of soybean, water lily, and psilicillus.

Another aspect of the present invention provides a cosmetic composition comprising the composition.

The composition according to one aspect of the present invention is characterized in that the composition comprises at least one of bamboo leaves and green tea obtained by enzymatic treatment of at least one of bamboo leaves and green tea as an active ingredient to obtain a transglutaminase- And has excellent skin barrier function and skin moisturizing effect. The composition according to another aspect of the present invention is characterized in that at least one of bamboo leaves and green tea is treated with an enzyme, and at least one of bamboo leaves and green tea obtained by treating with an enzyme, Thereby further promoting the synthesis of Transglutaminase-1, thereby having a better skin barrier function and skin moisturizing effect. The composition according to another aspect of the present invention further comprises at least one extract selected from the group consisting of soybean, water lily, and paulsikcho, thereby exhibiting a synergistic effect and having a better skin barrier function and skin moisturizing effect.

FIG. 1 is a graph showing the change (%) of the TEWL according to time after applying the composition according to the present invention to the skin.
FIG. 2 is a graph showing the amount of skin moisture over time after applying the composition according to the present invention to the skin. FIG.

As used herein, the term "extract" refers to a substance obtained by extracting a component contained therein from a natural product, regardless of the amount and kind of the component. For example, it is a broad concept that includes all components including extracts of components dissolving or decomposing from natural products using a solvent or an enzyme, extracts of only specific components such as oil, and specific components of other natural products.

As used herein, the term "skin" refers to a tissue covering the body surface of an animal, and is a concept of the broadest notion including a scalp and hair as well as a tissue covering a body surface such as a face or a body.

Hereinafter, the present invention will be described in detail.

One aspect of the present invention provides a skin moisturizing composition comprising, as an active ingredient, an extract of at least one of bamboo leaves and green tea obtained by enzymatically treating at least one of bamboo leaves and green tea. Another aspect of the present invention relates to a method for producing an extract of bamboo leaves and green tea obtained by treating at least one of bamboo leaves and green tea with an enzyme treatment, before enzyme treatment, after enzyme treatment or at an enzyme treatment with a pressure of 400 to 800 MPa, Which is contained as an active ingredient.

The composition for skin moisturizing according to one aspect of the present invention comprises at least one extract of bamboo leaves and green tea obtained by enzymatic treatment of at least one of bamboo leaves and green tea as an active ingredient. As described above, the method of obtaining an extract by enzyme treatment can produce an extract containing various kinds and a large amount of an effective ingredient by treating the enzyme, unlike other general extraction methods. In another aspect of the present invention, bamboo leaves and green tea extracts may be obtained by mixing extracts obtained by enzymatic treatment of bamboo leaves and green tea, or by enzymatic treatment of bamboo leaves and green tea together.

The composition for skin moisturizing according to another aspect of the present invention is characterized in that at least one of bamboo leaves and green tea is treated with an enzyme before treatment, after treatment with an enzyme, or at a high pressure, for example, a pressure of 400 to 800 MPa Decomposing, extracting at least one of bamboo leaves and green tea obtained as an active ingredient. When the high pressure treatment is performed in addition to the enzyme treatment, other general extraction methods as well as extracts containing a greater amount of various kinds and potency components than the enzyme treatment alone can be produced. Among them, when enzymes are treated at a high pressure, an extract containing the most various kinds and a large amount of an effective ingredient can be produced.

Particularly, the extract of at least one of bamboo leaves and green tea obtained by enzyme treatment and selectively high-pressure treatment contains a large amount of ingredients promoting the synthesis of Transglutaminase-1. Since transglutaminase-1 is an essential element for the formation and maintenance of the stratum corneum, promoting the synthesis of transglutaminase-1 can strengthen skin barrier function and protect skin moisture. Thus, the extract of one or more of bamboo leaves and green tea obtained by enzymatic treatment and optionally high pressure treatment can further enhance skin barrier function and have better skin moisturizing effect.

Hereinafter, a method for obtaining an extract by enzymatic treatment of the above plant will be described in more detail as follows:

In one aspect of the present invention, a method of enzymatically treating a plant to obtain an extract comprises enzymatically treating the plant to degrade the plant.

In one aspect of the present invention, the enzyme is at least one selected from the group consisting of amylase, protease, glycosidase, lactase, sucrose, and maltase. .

In one aspect of the invention, the enzyme treatment temperature can be adjusted according to the active temperature range of the enzyme used. In another aspect of the invention, the enzyme treatment temperature can be 30-60 < 0 > C. In another aspect of the invention, the enzyme treatment temperature may be 35-40 < 0 > C. If the enzyme treatment temperature is less than 30 ° C, the enzyme is difficult to activate. If the enzyme treatment temperature exceeds 60 ° C, most of the enzyme is destroyed and the function thereof is lost.

In one aspect of the invention, the weight ratio of plant to enzyme can be 100,000: 1 to 100: 1. If the weight ratio of the plant and the enzyme is less than 100,000: 1, the amount of the enzyme is too small to extract the effective component. If the weight ratio of the plant and the enzyme is more than 100: 1, the amount of the increase in the effective ingredient is insufficient.

In one aspect of the present invention, a method for obtaining an extract by enzymatic treatment of a plant may further include a step of treating the plant at a high pressure prior to the step of decomposing the plant by enzymatic treatment. In another aspect of the present invention, a method of obtaining an extract by enzymatic treatment of a plant may further include a step of treating the plant at a high pressure after the step of enzymatically treating and decomposing the plant. According to another aspect of the present invention, in the method of obtaining an extract by enzymatic treatment of a plant, the step of enzymatically treating and decomposing the plant may be an enzyme treatment of the plant at a high pressure, and this method may be the most preferable.

In one aspect of the invention, the high pressure can mean 400 to 1000 MPa. In another aspect of the invention, the high pressure can mean 400 to 800 MPa. In another aspect of the invention, the high pressure can mean 600 MPa, which is similar to the underwater pressure at a depth of 6,000 m. The pressure of the above range may be preferable since the efficacious components are not sufficiently extracted at a pressure of less than 400 MPa and the increase amount of extracting the efficacious ingredient is higher than that at a pressure of 800 MPa is inefficient.

In one aspect of the present invention, a method of enzymatically treating a plant to obtain an extract may further include a step of enzymatically treating the plant and then filtering the decomposed solution.

In one aspect of the present invention, a plant extract obtained by removing impurities by filtration of a solution obtained by enzymatic treatment can be obtained. In another aspect of the present invention, the filtration can be carried out by any method commonly used in the art, and specifically filtration through a microfilter can be used. Through this filtration, impurities can be removed to obtain purified plant extracts.

In one aspect of the present invention, a method of enzymatically treating a plant to obtain an extract may further comprise diluting the filtered solution after filtering the decomposed solution.

In one aspect of the invention, the filtered plant extract may be diluted in a suitable solvent for ease of use. In another aspect of the present invention, the solvent includes all solvents commonly used in the art, and specifically includes water, butyleneglycol, a mixed solvent thereof, and the like.

In one aspect of the present invention, the method of obtaining the extract by enzymatic treatment of the above-mentioned plant is applicable to all plants which produce the extract by a general extraction method. In another aspect of the present invention, the plant includes at least one member selected from the group consisting of bamboo leaves, green tea, beans, water lily, and paulownia. In another aspect of the present invention, the plant comprises at least one of bamboo leaves and green tea.

One aspect of the present invention provides a skin moisturizing composition comprising, as an active ingredient, an extract of at least one of bamboo leaves and green tea obtained by treating at least one of bamboo leaves and green tea at high pressure. In another aspect of the present invention, the high pressure may mean 400 to 1000 MPa. In another aspect of the invention, the high pressure can mean 400 to 800 MPa. In another aspect of the invention, the high pressure can mean 600 MPa.

One aspect of the present invention relates to a skin moisturizing composition comprising at least one extract of bamboo leaves and green tea obtained by extracting one or more of bamboo leaves and green tea with a general extraction method, for example, a solvent extraction method, to provide. Another aspect of the present invention is to provide a skin moisturizing composition comprising, as an active ingredient, an extract of at least one of bamboo leaves and green tea obtained by treating the enzyme before, after, or after enzyme treatment at a high pressure, for example, 400 to 800 MPa ≪ / RTI >

As used herein, general extraction methods include all conventional extraction methods in the art. Specifically, it may be a solvent extraction method in which each plant is leached into water or an organic solvent, purified and separated. The organic solvent is not particularly limited and may be a C ₁ -C ₅ lower alcohol, ether, ethyl acetate or chloroform. The C ₁ -C ₅ lower alcohol may be any one or a mixture of two or more selected from the group consisting of methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol and isobutanol .

The composition for skin moisturizing according to one aspect of the present invention may further comprise one or more extracts selected from the group consisting of soybean, water lily, and psilicillus. The extract of at least one selected from the group consisting of soybean, water lily, and psilicarpus also has a skin moisturizing effect. When the extract is contained together with at least one of bamboo leaves and green tea obtained by the above enzyme treatment, Skin barrier function enhancement and skin moisturizing effect.

In the above, bamboo leaves mean the leaves of bamboo ( Bambusoideae ), which are collectively referred to as evergreen perennial plants belonging to the lemonaceous plants (rice) and bamboo asparagus plants. Green tea refers to leaves of unfermented tea ( Camellia sinensis ). Soybean ( Glycine max ) is an annual plant of dicotyledonous plant rosemary bean, and water lily ( Nymphaea teragona ) is a generic term for dicotyledonous plants, buttercups, and lily plants. In one aspect of the present invention, the soybean, water lily or Opuntia species Coccinellifera ) comprises at least one of all the respective sites. For example, the bean, water lily or pomegranate may comprise one or more of each leaf, flower, stem, root, fruit and seed.

In the composition for skin moisturizing according to one aspect of the present invention, the extract of at least one of bamboo leaf and green tea may each be contained in an amount of 0.001 to 10.0% by weight based on the total weight of the whole composition. In the composition for skin moisturizing according to another aspect of the present invention, the extract of at least one of bamboo leaves and green tea may each contain 0.1 to 5.0% by weight based on the total weight of the whole composition.

In the composition for moisturizing the skin according to one aspect of the present invention, at least one extract selected from the group consisting of soybean, water lily, and psilicus can be contained in an amount of 0.001 to 10.0% by weight based on the total weight of the whole composition. In the skin moisturizing composition according to another aspect of the present invention, one or more extracts selected from the group consisting of soybean, water lily, and psilicus can be contained in an amount of 0.1 to 5.0% by weight based on the total weight of the total composition, respectively.

When used in the above range, it is not only suitable for exhibiting the intended effect of the present invention but also can satisfy all the stability and safety of the extract, and may be suitably used within the above range in terms of cost effectiveness. More specifically, when the extract is contained in an amount of less than 0.001% by weight, the skin moisturizing effect by the extract is insignificant. When the extract is contained in an amount exceeding 10% by weight, the effect increase rate is low and it may be ineffective.

One aspect of the present invention relates to a cosmetic composition comprising at least one extract of bamboo leaves and green tea obtained by enzymatic treatment and optionally at least one extract selected from the group consisting of green tea, soybean, water lily, to provide.

The cosmetic composition may be, for example, a cosmetic composition, and the cosmetic composition includes a cosmetically or dermatologically acceptable medium or base. It may be any formulation suitable for topical application, for example, a solution, a gel, a solid, an anhydrous product of a paste, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing a water phase in an oil phase, a multi-emulsion, Capsules, microgranules, ionic (liposomes) and non-ionic follicular dispersants, foams or compressed propellants. These compositions may be prepared according to conventional methods in the art.

The cosmetic compositions may also be in the form of tablets or capsules prepared by dissolving or dispersing the active ingredient in a diluent such as a lipid, an organic solvent, a solubilizer, a thickener, a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a foaming agent, , A chelating agent, a preservative, a vitamin, a barrier, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, a lipid vesicle or an adjuvant conventionally used in the field of cosmetic or dermatological science have. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

The formulation of the cosmetic composition is not particularly limited, and the formulation can be appropriately selected according to the purpose. For example, softeners, convergent lotions, nourishing lotions, nutritional creams, massage creams, essences, eye creams, eye essences, cleansing creams, cleansing lotions, cleansing foams, cleansing waters, packs, powders, cone sticks, body lotions, Hair lotion, hair conditioner, hair conditioner, hair shampoo, hair lotion, toothpaste, mouthwash, regular detergent, body lotion, body lotion, body lotion But are not limited to, one or more formulations selected from the group consisting of, for example, tablets, capsules, tablets, dragees, lotions, ointments, gels, creams, patches and sprays.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to Production Examples, Examples, Comparative Examples and Test Examples. However, these preparations, examples, comparative examples and test examples are provided for illustrative purposes only for the sake of understanding of the present invention, and the scope and scope of the present invention are not limited thereto.

[Production Examples 1 and 2]

Bamboo leaves and green tea were mixed and treated with amylase at 600MPa and 30 ~ 40 ℃, respectively. The enzyme-decomposed solution was filtered with a microfiltration paper to remove impurities. The filtered extract was diluted with a mixed solvent of water and butyleneglycol to prepare Preparations 1 and 2, respectively.

[Test Example 1] Evaluation of promoting transglutaminase-1 synthesis

In order to measure the skin moisturizing effect, bamboo leaf extract of the above-mentioned production example, bamboo leaf extract extracted using general solvent (ethanol) extraction method, and transglutaminase-1 synthesis of calcium chloride as a representative transglutaminase- The facilitating effects were compared.

First, human skin cell lines (available from Cascade Biologics) were placed in a 96-well plate incubator in an amount of 5 × 10 ⁴ per well and adhered for 24 hours. The adhered skin cell line was treated with the test substance and after 2 days, the medium was removed and stored in a refrigerator at -20 ° C. Cells treated with the substance were disrupted by repeating freeze-thawing twice, and then treated with acetone: ethanol (1: 1, v / v) stored at -20 ° C and left at 4 ° C for 30 minutes Cells were fixed. (1% bovine serum albumin), transglutaminase primary antibody and HRP anti-mouse antibody (secondary antibody) were carried out, and the OPD (o-phenyldiamine ) Was added to develop color. The absorbance was measured at 490 nm to calculate the expression level, and the correction was performed by measuring the background at 630 nm. The transglutaminase-1 synthesis promotion rate was calculated by comparing with the absorbance value of the untreated control group, and the results are shown in Table 1 below.

division Concentration (mg / ml) Synthesis promotion rate (%) of transglutaminase-1 High pressure enzyme treated bamboo leaf extract (Preparation Example 1) 12.5 137.0 ± 1.9 50 161.0 ± 4.0 Ethanol extract bamboo leaf extract 12.5 no effect 50 no effect Calcium chloride 1.5mM 169.9 ± 6.4

As shown in Table 1, it was confirmed that bamboo leaf extract obtained by enzymatic treatment at high pressure in the production example had an effect of promoting transglutaminase-1 synthesis, unlike bamboo leaf extract extracted by ethanol extraction method. In addition, when the bamboo leaf extract of Preparation Example was used at 50 mg / ml, it was confirmed that transglutaminase-1 synthesis promotion effect of about 95% compared with 1.5 mM of calcium chloride, which is a typical transglutaminase-1 synthesis promoting component .

[Comparative Examples 1 and 2 and Examples 1 to 6]

Comparative Examples 1 and 2 and Examples 1 to 6 including the composition shown in Table 2 below were prepared.

Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Bamboo leaf extract One - - - - - - - Green tea extract - One - - - - - - High pressure enzyme treated bamboo leaf extract (Preparation Example 1) - - One - - - - One High pressure enzyme-treated green tea extract (Preparation Example 2) - - - One - - - One Soybean extract - - - - One - - One Baekryikcho extract - - - - - One - One Water extract - - - - - - One One

In Table 2, the bamboo leaf extract, green tea extract, soybean extract, Baekryikcho extract and water extract are extracts extracted by the general solvent extraction method, and the high pressure enzyme treated bamboo leaves and green tea extract are the extracts obtained by enzymatic treatment at the high pressure of the above production example. The concentrations of all the extracts were the same at 1 wt% each based on the total weight of the composition.

[Test Example 2] TWEL evaluation

The compositions of Comparative Examples 1 and 2 and Examples 1 to 6 were applied to 20 adult males and 20 women and the TWEL value (Transepidermal Water Loss) was measured for 48 hours. TWEL values were evaluated at 6 hours, 24 hours and 48 hours when the TWEL value before application of the composition was taken as 100, and it is shown in Fig.

As can be seen from FIG. 1, the TWEL value was lower than that of Comparative Example 1 or 2 when Example 1 or 2 was applied, and the TWEL value was lowest when Example 6 was applied. Therefore, a composition containing all of bamboo leaves, green tea extract, soybean extract, Paekryikcho extract and water extract obtained by enzymatic treatment at a high pressure is low in the TEWL value of the extract obtained by enzymatic treatment at higher pressure than the extract obtained by general solvent extraction method The lowest TEWL value shows that the effect of preventing skin moisture loss is the best.

[Comparative Examples 3 to 5 and Examples 7 to 12]

The cosmetic compositions of Comparative Examples 3 to 5 and Examples 7 to 12 were prepared with the compositions shown in Table 3 below. The specific manufacturing method is as follows. First, the oil components in Table 3 were heated at 70 to 75 DEG C and mixed uniformly. Separately, Water component 1 in Table 3 was heated at 70 to 75 占 폚 and uniformly dissolved and mixed. The emulsion was formed by stirring the aqueous phase component 1 while maintaining the temperature at 70 to 75 캜 and injecting an oil phase component. Then, alcohol components were added and mixed, and the aqueous phase 2 was continuously added thereto, followed by cooling to 30 占 폚.

division Raw material name Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Comparative Example 3 Comparative Example 4 Comparative Example 5 Oil component Pentaerythritol tetrahexyl hexanoate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Vegetable squalane 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Cyclomethicone 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 PC ADMICICON 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Polysorbate 20 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Cyclopentasiloxane / dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Water component 1 Bamboo sap To100 To100 To100 To100 To100 To100 To100 To100 To100 Disodium iodide 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Hydrogenated lecithin / C12-16 alcohol / palmitic acid 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Dimethicone copolyol 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Propanediol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Pigment / PPE-17/6 Copolymer 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Methylglucose-20 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Tromethamine 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Betaine 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Bamboo extract - - - - - - 1.0 - - Green tea extract - - - - - - - 1.0 - High-pressure enzyme treatment bamboo leaf extract 1.0 1.0 - - - - - - - High pressure enzyme-treated green tea extract 1.0 - 1.0 - - - - - - Soybean extract 1.0 - - 1.0 - - - - - Baekryikcho extract 1.0 - - - 1.0 - - - - Water extract 1.0 - - - - 1.0 - - - Alcohol component ethanol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 antiseptic 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 incense 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Water component 2 Cabomar 1% Bamboo sap 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0

[Test Example 3] Change in skin moisture content

20 adult men and women considered to be dry skin were selected and the cosmetic compositions of Examples 7 to 12 and Comparative Examples 3 to 5 were applied to the entire skin after one hour of standing under constant temperature and humidity conditions. Skin moisture was measured before application, immediately after application, 4 hours, 8 hours and 24 hours after application, and the results are shown in FIG.

As can be seen from FIG. 2, when Example 7 was applied, it was confirmed that there was almost no change in skin moisture content after 24 hours of application and immediately after application. On the other hand, in Comparative Example 3, it was confirmed that the skin moisture amount after 24 hours was significantly reduced as compared with immediately after application. That is, the moisturizing effect of a cosmetic composition containing at least one of bamboo leaves and green tea obtained by high-pressure enzyme treatment is superior to the extract obtained by a general solvent extraction method, and is further improved when the extract contains soybean extract, Baekryikcho extract and water extract can confirm.

Formulation examples of compositions according to one aspect of the present invention are described below, but may be applied to various other formulations, which are not intended to be limiting but merely illustrative of the invention.

[Formulation Example 1] Lotion

ingredient weight% Example 6 5.0 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Purified water Balance

[Formulation Example 2] Cream

Compounding ingredient Content (% by weight) Example 6 1.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] ointment

ingredient weight% Caprine / caprylic triglyceride 10 Liquid paraffin 10 Sorbitan sesquioleate 6 Octyldodec-25 9 3-hexyl hexanoate 10 Squalane One Salicylic acid One glycerin 15 Sorbitol One Example 6 2 Preservative, coloring, fragrance Suitable amount Purified water Balance

Claims (8)

An extract of bamboo leaves obtained by enzymatic treatment of bamboo leaves as an active ingredient,
Wherein the extract is obtained by treating bamboo leaves at a pressure of 400 to 800 MPa before the enzyme treatment, after the enzyme treatment or after the enzyme treatment.
A cosmetic composition for skin moisturizing. delete The method according to claim 1,
Wherein the enzyme is at least one selected from the group consisting of protease, glycosidase, lactase, sucrose, and maltase. The method according to claim 1,
Wherein the enzyme treatment temperature is 30 to 60 占 폚. The method according to claim 1,
Wherein the weight ratio of the bamboo leaves to the enzyme is 100,000: 1 to 100: 1, respectively. The method according to claim 1,
Wherein the bamboo leaf extract is contained in an amount of 0.001 to 10.0% by weight based on the total weight of the whole composition. delete The method according to claim 1,
Wherein the extract of bamboo leaves promotes the synthesis of transglutaminase-1.

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