KR20140110376A - Skin external composition comprising flower vinegar extract - Google Patents

Abstract

The present invention relates to a skin external composition containing flower vinegar extract and, more specifically, to a skin external composition containing at least one of amellia japonica vinegar extract and Nymphaea alba vinegar extract. By containing at least one of Camellia japonica vinegar extract and Nymphaea alba vinegar extract, the skin external composition has an excellent effect in smoothing out skin wrinkles, tightening pores, reducing sebum, improving acne, and whitening.

Description

[0001] The present invention relates to a skin external composition comprising a flower vinegar extract,

The present invention relates to a skin external composition containing kkotcho extract, more specifically, camellia (Camellia japonica ) vinegar extract and water lily ( Nymphaea Alba ) vinegar extract. More specifically, it relates to a composition for external application for skin containing at least one of camphor vinegar extract and vinegar extract, which can improve skin wrinkles, improve skin elasticity, reduce pores, The present invention relates to a composition for external application for skin having excellent acne improvement and whitening effect.

Vinegar is a kind of seasoning that produces a sour taste. Since it is produced through acetic acid fermentation, it contains acetic acid and has been usefully used as a foodstuff as a preservative. These vinegars are classified into brewing vinegar and synthetic vinegar according to the production method. The vinegar vinegar is produced by brewing method. According to the raw materials, it is divided into cereals and fruit orchards and classified into pure brewing or alcoholic beverages according to the fermentation process . Synthetic vinegar is produced by a chemical process, including the addition of synthetic vinegar to pure synthetic vinegar and vinegar vinegar.

Camellia according to the present invention japonica ) or water lily ( Nymphaea Alba ) as a vinegar extract by acetic acid fermentation has not been studied or reported on skin.

Korean Patent Laid-Open No. 10-2010-0057460

The present inventors camellia (Camellia japonica ) vinegar extract and water lily ( Nymphaea Alba ) vinegar extract exhibits remarkably excellent effects on skin elasticity enhancement, skin wrinkle reduction, pore reduction, sebum control and whitening effect, and the present invention has been completed.

Accordingly, an object of the present invention is to provide a composition for external application for skin, which contains at least one of camellia vinegar extract and vinegar extract to enhance skin elasticity, improve skin wrinkles, reduce pores, control sebum and whitening effect.

In order to achieve the above object, the present invention provides an external preparation for skin comprising at least one of camellia vinegar extract and vinegar extract.

Further, the present invention provides a composition for external application for skin for improving skin wrinkles containing at least one of camellia vinegar extract and vinegar vinegar extract.

The present invention also provides a composition for external skin application for skin elasticity enhancement containing at least one of camellia vinegar extract and water vinegar extract.

Further, the present invention provides a composition for external application for skin for reducing pores of at least one of camphor vinegar extract and vinegar extract.

The present invention also provides a composition for external application for skin reduction comprising at least one of camellia vinegar extract and water vinegar extract.

The present invention also provides a composition for external application for skin for acne improvement comprising at least one of camellia vinegar extract and water vinegar extract.

The present invention also provides a skin external composition for skin whitening comprising at least one of camellia vinegar extract and water vinegar extract.

The composition according to the present invention contains at least one of camellia vinegar extract and vinegar extract to provide skin wrinkle improvement, skin elasticity improvement, pore reduction, sebum reduction, acne improvement and skin whitening effect, When mixed extract of vinegar vinegar extract is contained, it exhibits remarkably excellent effect through synergistic effect. Provided is a composition for external application for skin which shows remarkably excellent efficacy in improving wrinkles of skin, improving skin elasticity, reducing pores, reducing sebum, improving acne and skin whitening effect, in comparison with general camellia extract, common water extract or combination thereof .

The composition for external application for skin according to the present invention comprises Camellia japonica ) vinegar extract and water lily ( Nymphaea Alba ) vinegar extract as an active ingredient.

Camellia (Camellia to be used in the present invention japonica ) is an evergreen tree of the tea plant, and it is mainly distributed in East Asia including Korea. Seed oil is used to make camellia oil, and wood is used as furniture, handwork, and so on. In the present invention, the camellia can be used in all parts of plants such as roots, stems, leaves and flowers, and can be used as a whole plant (outpost).

The water lily ( Nymphaea Alba ) is a water lily and aquatic plant, also known as white water lily, contains an alkaloid component and is used as a sedative. Roots, stems, and flowers have been used as medicines in traditional medicine. In the present invention, the water lily may use all parts of the plant such as roots, stems, leaves, flowers, and the like as whole plants (outpost).

The camellia vinegar extract according to the present invention means that the camellias are obtained by acetic acid fermentation. The vinegared vinegar extract may be prepared through saccharification, alcohol fermentation and acetic acid fermentation.

In one embodiment of the present invention, the camellia vinegar extract may be contained in an amount of 0.001% by weight or more, preferably 10.0% by weight or more, based on the total weight of the composition. In another embodiment of the present invention, the camellia vinegar extract may be contained in an amount of 90.0% by weight or less based on the total weight of the composition, and preferably in an amount of 70.0% by weight or less. In another embodiment of the present invention, the camellia vinegar extract may be contained in an amount of 0.001% by weight or more and 90.0% by weight or less, more preferably 10% by weight or more and 70.0% by weight or less, ≪ / RTI > When it is used in an amount of less than 0.001% by weight, its effect can not be sufficiently exhibited. When it is used in an amount exceeding 90.0% by weight, the pH is low and there is a fear of skin irritation.

The vinegar extract according to the present invention means that the above-mentioned training is obtained by acetic acid fermentation. The vinegar extract of the present invention can be prepared through saccharification, alcohol fermentation and acetic acid fermentation.

In one embodiment of the present invention, the water vinegar extract may be contained in an amount of 0.001% by weight or more, preferably 10.0% by weight or more, based on the total weight of the composition. In another embodiment of the present invention, the water vinegar extract may be contained in an amount of 90.0% by weight or less, preferably 70.0% by weight or less, based on the total weight of the composition. In another embodiment of the present invention, the vinegar extract may be contained in an amount of 0.001% by weight or more and 90.0% by weight or less, more preferably 10% by weight or more and 70.0% by weight or less, ≪ / RTI > When it is used in an amount of less than 0.001% by weight, its effect can not be sufficiently exhibited. When it is used in an amount exceeding 90.0% by weight, the pH is low and there is a fear of skin irritation.

In one embodiment of the present invention, when the camellia vinegar extract and the water vinegar extract are contained, the camellia vinegar extract and the water vinegar extract may be contained at a weight ratio of 2: 8 to 8: 2, May be contained in a ratio of 4: 6 to 6: 4. When the ratio is out of the range of 2: 8 to 8: 2, only one effect is concentrated, and the synergy of the effect is not sufficiently exerted. When the camellia vinegar extract and the vinegar extract are simultaneously contained as an active ingredient, the total content of the active ingredient may be 0.001% by weight or more and 90.0% by weight or less, preferably 10% by weight or more and 90.0% by weight or less ≪ / RTI > When it is used in an amount of less than 0.001% by weight, its effect can not be sufficiently exhibited. When it is used in an amount exceeding 90.0% by weight, the pH is low and there is a fear of skin irritation.

The composition according to the present invention can be used as a skin external application composition for enhancing skin elasticity and can be used as a skin external application composition for skin wrinkle improvement. This is excellent in promoting collagen synthesis of skin, improving skin elasticity to improve skin wrinkles, and improving skin deflection to provide a lifting effect.

The composition according to the present invention can be used as a composition for external application for skin shrinkage and sebum control, and it is excellent in suppressing excessive sebum secreted in skin and reducing the pore size.

The composition according to the present invention can be used as a composition for external application for skin for improving acne, has an excellent antimicrobial effect against acne causing bacteria, and is excellent in skin trouble-suppressing effect.

The composition according to the present invention can be used as a composition for external skin application for skin whitening, which can provide an excellent whitening effect by inhibiting tyrosinase activity and inhibiting the production of melanin.

The composition according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic form (liposome) In the form of ionic fibrin dispersions, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening agents, gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, Such as fillers, emulsifiers, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants commonly used in the field of cosmetics or dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to test examples and formulation examples. However, these test examples and formulation examples are provided for illustrative purposes only for the sake of understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Production Example 1] Preparation of camellia vinegar extract

1. Bone making process (saccharification process)

After washing 20 kg of brown rice, immerse in 30 kg of mineral water (160 m bedrock) for 5 hours, prepare water by taking out and prepare wild camelli flower by taking, cutting, washing and removing water. (Steamed) at a temperature of ~ 110 ° C for 25 to 35 minutes and then cooled to 60 ° C. 2 kg of malt flour is added to the cooled brown rice and sugar is sacrificed for 12 hours. 6 kg of wheat nuruk were added to the mixture and saccharified at a temperature of 30 ° C for 48 hours to activate myrrh. The resulting syrup was placed in a jar and 40 kg of mineral water (160 m 2) was added. The mixture was air-heated at 25 ° C for 72 hours And only the carbon dioxide is released and maintained to manufacture the bone graft.

2. Alcohol fermentation process

After washing 13 kg of brown rice, it is immersed in 20 kg of water for one hour, and then 1 kg of brown rice and camellia remaining after the water is removed is boiled at a temperature of 90 to 110 ° C for 25 to 35 minutes to prepare a rice cake. 10 kg of the above-prepared gangbang and camellia flower are added to the beads prepared in the above 1., and the alcohol is fermented at 25 ° C for 10 to 15 days.

3. Acetic Acid Fermentation Process

The fermented product obtained in 2 above is diluted by adding 1 kg of water. The inlet is sealed with a cloth so that air can enter the jar at 27 ° C for 2 months, and oxygen is supplied while occasionally stirring to ferment primary (aerobic) acetic acid . The pH of the resulting fermentation product is measured (PH-230SD PH meter) to confirm that the pH is 4 or more. Only the supernatant is separated and matured at 5 to 20 ° C in a shaded, cool place. After that, the aged liquid was filtered to obtain a camellia vinegar extract. The obtained camellia vinegar extract was sterilized and bottled and stored in a cool dark place, which was provided in the following test.

[Preparation Example 2] Preparation of water vinegar extract

1. Bone making process (saccharification process)

After washing 20 kg of brown rice, immerse in 30 kg of mineral water (160 m bedrock) for 5 hours, then prepare it by taking out water, and prepare wild ladies by taking, cutting, washing and removing water. The mixture is heated at 90 to 110 ° C for 25 to 35 minutes and then cooled to 60 ° C. 2 kg of malt flour is added to the cooled brown rice and sugar is sacrificed for 12 hours. 6 kg of wheat nuruk were added to the mixture and saccharified at a temperature of 30 ° C for 48 hours to activate myrrh. The resulting syrup was placed in a jar and 40 kg of mineral water (160 m 2) was added. The mixture was air-heated at 25 ° C for 72 hours And only the carbon dioxide is released and maintained to manufacture the bone graft.

2. Alcohol fermentation process

After washing 13 kg of brown rice, it is immersed in 20 kg of water for one hour and then 1 kg of brown rice and water liquor remaining after water is added is boiled for 25 to 35 minutes at a temperature of 90 to 110 ° C to prepare a rice cake. 10 kg of the above-prepared soup and liquor are added to the bees prepared in the above 1. and the alcohol is fermented at 25 ° C for 10-15 days.

3. Acetic Acid Fermentation Process

The fermented product obtained in 2 above is diluted by adding 1 kg of water. The inlet is sealed with a cloth so that air can enter the jar at 27 ° C for 2 months, and oxygen is supplied while occasionally stirring to ferment primary (aerobic) acetic acid . The pH of the resulting fermentation product is measured (PH-230SD PH meter) to confirm that the pH is 4 or more. Only the supernatant is separated and matured at 5 to 20 ° C in a shaded, cool place. After that, the aged liquid was filtered to obtain a vinegar extract of liquified. The obtained vinegar extracts were sterilized and bottled and stored in a dark place.

[Comparative Preparation Example 1] Preparation of camellia extract

In the camellia extract of the present invention, 19 g of camelliaceae was mixed with 10 g of butylene glycol and 71 g of purified water, followed by heating and extraction for 1 hour. Thereafter, the mixture was centrifuged at 8000 rpm at 15 DEG C for 30 minutes, and then sequentially filtered with 3.00 mu m, 1.2 mu m, 0.7 mu m and 0.45 mu m filters. The extracted stock solution was suspended in an auto mixer at 800 rpm for 1 hour to contain 10% butylene glycol.

 [Comparative Preparation Example 2] Preparation of water extracts

In the water extract of the present invention, 10 g of butylene glycol and 71 g of purified water were mixed with 19 g of the water treatment, and the mixture was heated and extracted for 1 hour. Thereafter, the mixture was centrifuged at 8000 rpm at 15 DEG C for 30 minutes, and then sequentially filtered with 3.00 mu m, 1.2 mu m, 0.7 mu m and 0.45 mu m filters. The extracted stock solution was suspended in an auto mixer at 800 rpm for 1 hour to contain 10% butylene glycol.

[Examples 1 to 3 and Comparative Examples 1 to 4]

Examples 1 to 3 and Comparative Examples 1 to 4 were prepared according to the compositions shown in the following Table 1 using the above Production Examples 1 and 2 and Comparative Production Examples 1 and 2 (unit: wt%).

Raw material name Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Water component The camellia vinegar extract (Preparation Example 1) 78 - 39 - - - - Water vinegar extract (Preparation Example 2) - 78 39 - - - - Camellia extract (Comparative Production Example 1) - - - - 78 - 39 Water extract (Comparative Preparation Example 2) - - - - - 78 39 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 Disodium iodide 0.02 0.02 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Xanthan gum 0.15 0.15 0.15 0.15 0.15 0.15 0.15 Methylglucose-20 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glycereth-26 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Ammonium acryloyldimethyltaurate / Vpicopolymer 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Ethanol phase component ethanol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Hydrogenated castor oil 0.3 0.3 0.3 0.3 0.3 0.3 0.3 incense 0.1 0.1 0.1 0.1 0.1 0.1 0.1 antiseptic 0.3 0.3 0.3 0.3 0.3 0.3 0.3

<Manufacturing Method>

(1) The aqueous phase components (1 to 9) were uniformly mixed at room temperature.

(2) Ethanol phase components (10 to 13) were heated to 50 to 55 占 폚 and uniformly dissolved and mixed.

(3) The above-mentioned (1) was added to the above-mentioned (2) under agitation to uniformly solubilize.

[Test Example 1] Skin elasticity improvement effect

In order to confirm the skin elasticity improving effect according to the present invention, the following tests were conducted using the above-described Examples 1 to 3 and Comparative Examples 1 to 4. (Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4) The skin elasticity improvement effect was measured using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany). The results are shown in Table 2 below. The results in Table 2 indicate the viscoelasticity of the skin elasticity measuring device.

Test substance Skin elasticity effect Example 1 0.66 + 0.10 Example 2 0.70 + - 0.10 Example 3 0.75 + - 0.10 Comparative Example 1 0.35 + 0.09 Comparative Example 2 0.46 ± 0.09 Comparative Example 3 0.44 ± 0.09 Comparative Example 4 0.48 ± 0.09

In the results shown in Table 2, in the case of Comparative Examples 2 to 4 containing common camellia extract, common water extract or a combination thereof, skin elasticity was enhanced by about 25 to 37% compared with Comparative Example 1 containing no extract . However, when Examples 1 to 3 containing camellia vinegar extract, water vinegar extract, or a combination thereof were used, the skin elasticity was improved by 188 to 215% compared to Comparative Example 1, indicating that the extract exhibited remarkably excellent efficacy. In particular, when the camellia vinegar extract and the water vinegar extract are simultaneously contained, the skin elasticity enhancement effect is more remarkably improved through the synergistic effect.

[Test Example 2] Collagen synthesis promotion effect

Human fibroblasts were cultured in a 24-well plate culture incubator and then mixed extracts of the camellia vinegar extract, water vinegar extract, camellia vinegar extract and water vinegar extract of Preparation Examples 1 and 2 at the concentrations shown in Table 3, 1 to 2 of common camellia extract, common water lily extract, and mixed extract of common camellia and water lily extract. On the third day of culture, 0.5 ml of DMEM medium containing 10% fetal bovine serum was added to each well, and 10 [mu] Ci of L [2,3,4,5- ³ H] -proline was added. After 24 hours, the media and cells in each well were scraped off, washed with 5% trichloroacetic acid (TCA) solution, and then divided into two test tubes. One test tube contained Type I collagen One unit / ㎕ of type I collagenase was added, incubated at 37 ° C for 90 minutes, and the other tubes were stored at 4 ° C. Then, 0.05 ml of 50% trichloroacetic acid was added to all the tubes, and the mixture was allowed to stand at 4 ° C for 20 minutes, and then centrifuged at 12,000 rpm for 10 minutes at each time. The supernatant and the precipitate were separated by a liquid scintillation counter Counter (CPM) values were obtained and relative collagen biosynthesis (RCB) was measured for the control group and the experimental group according to the following equation (1), and the results are shown in Table 3 below .

Camellia
Vinegar extract
(Production Example 1) training
Vinegar extract
(Production Example 2) Camellia extract
(Comparative Production Example 1) Water extract
(Comparative Production Example 2) Collagen Biosynthetic Rate (RCB) (%) Control group
(Untreated group) - - - - 100 Example 4 10 - - - 148 Example 5 - 10 - - 145 Example 6 5 5 - - 152 Comparative Example 5 - - 10 - 125 Comparative Example 6 - - - 10 122 Comparative Example 7 - 5 5 126

In the results shown in Table 3, in the case of Comparative Examples 5 to 7 containing the common camellia extract, the common water extract or a combination thereof, the collagen biosynthesis rate was about 22 to 26% as compared with the control without extract. However, when Examples 4 to 6 containing the camellia vinegar extract, water vinegar extract, or a combination thereof were used, the collagen biosynthesis ratio was about 45 to 52% as compared with the control, indicating remarkably excellent efficacy. Particularly, when the camellia vinegar extract and the water vinegar extract are simultaneously contained, the collagen biosynthesis ratio is remarkably superior due to the synergistic effect.

[Test Example 3] Sensory evaluation of lifting efficacy

And 35 women aged 25 to 35 years who were worried about skin sagging were divided into seven groups of five each, and the lotions of Examples 1 to 3 and Comparative Examples 1 to 4 were used for four weeks in the same manner as general lotion, After 1 week from the start of use, the level of user's skin lifting efficacy was evaluated by questionnaire according to the following evaluation criteria. The results are shown in Table 4 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: There is a slight improvement.

±: No improvement, but no deterioration.

-: No improvement, but rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 weeks Example 1 ++ ++ +++ +++ Example 2 ++ ++ ++ +++ Example 3 ++ +++ +++ +++ Comparative Example 1 ± ± + + Comparative Example 2 + + ++ ++ Comparative Example 3 + + + ++ Comparative Example 4 + + ++ ++

In the results of Table 4, compared with Comparative Examples 1, 2, 3, and 4, which contain no extracts, the extracts of camellia vinegar, water vinegar Examples 1 to 3 containing extracts and mixed extracts thereof exhibited rapid and excellent lifting efficacy.

[Test Example 4] Pore size reduction efficacy

Forty-five women aged 30 or older, who were suffering from pore problems, were divided into three groups of 15 individuals each. Examples 1 to 3 were applied daily for two weeks in a prominent pore area. The pore size was measured by a pore analysis program (manufactured by Tru System). The average pore size was divided into three grades by a pore analysis program, and the number of each group was counted, and the results are shown in Table 5 below.

sample Average pore size
(pixel) Pore size
Large group (persons) Pore size
Intermediate group (persons) Pore size
Small group (persons) Example
One Before the test 195.3 6 5 4 One week after the test 185.0 5 6 4 Two weeks after the test 162.3 4 5 6 Example
2 Before the test 189.1 7 5 3 One week after the test 180.0 5 7 3 Two weeks after the test 155.3 4 5 6 Example
3 Before the test 197.5 6 4 5 One week after the test 181.6 5 4 6 Two weeks after the test 145.6 3 5 7

From the results of Table 5, it can be seen that the composition according to the present invention can effectively reduce the size of pores.

[Test Example 5] Sensory evaluation of pore reduction effect

70 women aged 25 to 35 years who were worried about large pores were divided into seven groups of ten each, and the lotions of Examples 1 to 3 and Comparative Examples 1 to 4 were used for four weeks in the same manner as general lotion. The degree of skin improvement was judged according to the following evaluation criteria, and the results are shown in Table 6 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: There is a slight improvement.

±: No improvement, but no deterioration.

-: No improvement, but rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 weeks Example 1 ++ ++ +++ +++ Example 2 ++ +++ +++ +++ Example 3 ++ +++ +++ +++ Comparative Example 1 + + + ++ Comparative Example 2 + ++ ++ ++ Comparative Example 3 + ++ ++ ++ Comparative Example 4 + ++ ++ +++

In the results of Table 6 above, it can be seen that compared with Comparative Examples 1, 2, 3 and 4, which contain no extract at all and Comparative Examples 1, 2, 3 and 4, Examples 1 to 3 containing extracts and mixed extracts thereof exhibited a quick and excellent pore-shrinking effect.

[Test Example 6] Confirming efficacy of reducing sebum amount

The subjects were divided into three groups each consisting of 10 men and 30 women in their 20s and 30s who felt that sebum secretion was high. Before the start of the test and two weeks after the test, the sebum amount was measured using a skin oil analyzer (Sebumeter SM810) after 4 weeks of the test, and the results are shown in Tables 7 to 9 below.

sample Example 1 Subject Before test (㎍ / ㎠h) After 2 weeks (㎍ / ㎠ h) After 4 weeks (㎍ / ㎠ h) One 90 85 79 2 86 82 80 3 102 96 85 4 103 82 76 5 94 85 79 6 74 67 60 7 68 53 44 8 65 51 50 9 86 82 74 10 59 56 47

sample Example 2 Subject Before test (㎍ / ㎠h) After 2 weeks (㎍ / ㎠ h) After 4 weeks (㎍ / ㎠ h) One 95 81 75 2 92 85 78 3 74 70 65 4 52 45 40 5 94 86 82 6 74 68 60 7 106 99 82 8 65 55 50 9 68 56 43 10 103 95 74

sample Example 3 Subject Before test (㎍ / ㎠h) After 2 weeks (㎍ / ㎠ h) After 4 weeks (㎍ / ㎠ h) One 65 60 45 2 58 50 43 3 101 88 65 4 85 80 71 5 77 55 50 6 66 60 44 7 100 85 70 8 88 85 70 9 106 100 91 10 55 50 40

From the results of Tables 7 to 9, it can be confirmed that the composition according to the present invention can effectively inhibit sebum.

[Test Example 7] Sensory evaluation of acne improvement effect

Among the women aged 19 to 26, 35 persons with acne were divided into 7 groups of 5 persons each, and the lotions of Examples 1 to 3 and Comparative Examples 1 to 4 were used for 4 weeks in the same manner as general lotion. The degree of skin improvement according to the opinion was judged according to the following evaluation criteria. The results are shown in Table 10 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: There is a slight improvement.

±: No improvement, but no deterioration.

-: No improvement, but rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 weeks Example 1 ++ +++ +++ +++ Example 2 ++ ++ +++ +++ Example 3 +++ +++ +++ +++ Comparative Example 1 + + + ++ Comparative Example 2 + ++ ++ ++ Comparative Example 3 + ++ ++ ++ Comparative Example 4 + ++ ++ +++

In the results of Table 10 above, it can be seen that compared with Comparative Examples 1, 2, 3 and 4, which contain no extract at all and Comparative Examples 2 to 4 containing common extracts of Camellia, Examples 1 to 3 containing extracts and mixed extracts thereof showed rapid and excellent acne skin improving effect.

[Test Example 8] Whitening effect test on human skin

After attaching an opaque tape with 6 holes of 1.5 cm in diameter to the upper forearm of the subject in the group of 5 to 35 persons in each of the 30s to 40s for each 5 to 1.5 times the Minimal Erythema Dose of each subject (UVB) to induce blackening of the skin, and Example 1-3 and Comparative Examples 1 to 4 were applied to each of the holes every morning and evening twice each day, and after 50 days, using a colorimeter The darkness of the skin was measured.

The effect was judged by measuring the degree of blackness of the skin using a color difference meter (Minolta CR2002). L, a, and b colorimetric systems are used to display colors. In the present invention, "L" values representing skin darkness were calculated and analyzed (color values of unburned Korean skin are usually 50 to 70). L values were calibrated with a standard white plate, and measurements were made evenly by repeating the measurements five times or more per site.

When the test substance is applied, the L value gradually increases. When the test substance is applied, the L value gradually increases. The comparison between the test substances calculates the difference (ΔL) of skin color at the application start time and the completion time according to the following formula The results are shown in Table 11 below.

Test substance Whitening effect (? L) Example 1 4.22 Example 2 4.02 Example 3 5.04 Comparative Example 1 0.88 Comparative Example 2 1.92 Comparative Example 3 2.05 Comparative Example 4 2.56

In the results of Table 11, when Examples 1 to 3 containing a camellia vinegar extract, a water vinegar extract, or a combination thereof were used, compared with Comparative Examples 2 to 4 containing a common camellia extract, a common water extract or a combination thereof And a remarkable whitening effect of 1.5 to 3 times. In particular, when the camellia vinegar extract and the water vinegar extract are simultaneously contained, it is confirmed that the whitening effect is remarkably superior through the synergistic effect.

Claims (9)

Camellia japonica ) vinegar extract and water lily ( Nymphaea Alba ) vinegar extract as an active ingredient. The method according to claim 1,
Wherein the active ingredient is contained in an amount of 0.001 to 90% by weight based on the total weight of the composition. The method according to claim 1,
Wherein the composition is for improving skin wrinkles. The method according to claim 1,
Wherein the composition is for skin elasticity enhancement. The method according to claim 1,
Wherein the composition is for shrinking pores. The method according to claim 1,
Wherein the composition is for sebum reduction. The method according to claim 1,
Wherein the composition is for improving acne. The method according to claim 1,
Wherein the composition is for skin whitening. 9. The composition for external application for skin according to any one of claims 1 to 8, wherein the camphor vinegar extract and the water vinegar extract are contained in a weight ratio of 2: 8 to 8: 2.