KR101833274B1 - External composition for skin containing Nelumbo nucifera seed extract - Google Patents

Abstract

The present invention relates to a composition for external application for skin containing germinated lotus seed extract, and more particularly, to a composition containing germinated lotus seed extract extracted by a subcritical extraction method to exhibit an excellent skin moisturizing effect.

Description

[0001] The present invention relates to an external composition for skin containing Nelumbo nucifera seed extract,

The present invention relates to a composition for external application for skin containing a lotus seed extract, and more particularly to a composition for external application for skin, which contains an extract of lotus seeds and has excellent skin moisturizing effect.

Recently, many skin external composition compositions such as cosmetics using natural materials have been developed to solve the above problems in skin moisturizing effect and the like while reducing side effects of skin caused by various chemical substances and the like. Accordingly, the present inventors have studied the applicability of various natural products, which have not yet been widely known, as an external preparation for skin.

The lotus (Nelumbo nucifera) is attracting attention. Kite is a plant that has been used for a long time as a food or herb medicine because various parts such as root, stem, leaf, and fruit have been used for a long time and its safety is quite proven. It is said that in Kangwon Gangmok, the kite is energizing and defeats all diseases, and when taken for a long time, it becomes lighter and prolongs its life. In the medicinal herbaceous plant, the stamen lacks the energy of the five stomach muscles, especially the stomach of the heart, the rain, the god of the stomach and the stomach of the stomach. It is said to make it black and longevity. The lotus is mainly used for ornamental purposes, but its application has not been studied much, especially the leaves contain alkaloids and tannins, so they are used as astringent and hemostatic agents, or in the private sector for the treatment of nocturnal enuresis. The underground stem is called lotus root, it contains asparagine, it is edible and it is used as a nourishing medicine. Fruit and seeds are known to be good for women's diseases.

Studies on the technology for producing a cosmetic composition using a conventional starch have been conducted, but no research has been conducted on a cosmetic composition using lotus seeds. In addition, usually, the extract of Yan is extracted as a whole without separating into a flower, stem, root, or the like part, and the extraction effect is lowered. In addition, there is a problem that the manufacturing process is complicated by supplementing the effect by adding other substances .

Korean Patent No. 2011-0115047

The inventors of the present invention have confirmed through repeated and various experiments that the lotus seed extract can provide a sufficient moisturizing effect to the skin, and completed the present invention. Furthermore, the present inventors have found that the ginseng extract of lotus seeds exhibits a better moisturizing effect than that of ginseng lotus seed extract without germination, and that the extract of lotus seeds extracted by the subcritical extraction method is more excellent in moisturizing effect.

Accordingly, an object of the present invention is to provide a composition for external application for skin having an excellent skin moisturizing effect on the skin by containing the germinated lotus seed extract as an active ingredient. It is another object of the present invention to provide a composition for external application for skin having an excellent skin moisturizing effect on the skin by containing the extract of lotus seeds extracted by the subcritical extraction method.

In order to achieve the above object, the present invention provides a composition for external application for skin containing germinated lotus seed extract.

In addition, the present invention provides a composition for external application for skin containing lotus seed extract extracted by a subcritical extraction method so as to impart better moisture to skin.

The composition of the present invention provides a better skin moisturizing effect by containing a germinated lotus seed extract or a lotus seed extract extracted by a subcritical extraction method.

Further, the composition of the present invention can be used not only for skin moisturizing effect but also for skin aging, skin oxidation, skin elasticity improvement, skin wrinkle improvement, color improvement, pore shrinkage, skin irritation mitigation, homeostasis, elasticity, acne, regeneration, It is possible to provide an excellent effect even in the exfoliation improvement effect, skin convergence or tidiness effect.

Accordingly, the composition of the present invention can be used in various fields in the field of cosmetics or medicine.

1 is a graph showing a water content increase rate of a composition containing lotus seed extract, germination lotus seed extract, subcritical lotus seed extract and subcritical germination lotus seed extract according to the present invention and a composition not containing any of them .
FIG. 2 is a graph showing the TEWL (water-surface moisture loss) of a composition containing lotus seed extract, germination lotus seed extract, subcritical lotus seed extract and subcritical germination lotus seed extract according to the present invention, Fig.

The present invention relates to a lotus seed ( Nelumbo nucifera seed extract as an active ingredient. In addition, the present invention relates to a composition for external application for skin, which comprises a γ-linolenic acid containing a physiologically active substance as a source of essential fatty acids and thus can have a long lasting moisturizing effect on the skin.

More specifically, the lotus seed extract used in the present invention is extracted from the seeds of lotus flowers, preferably from germinated lotus seeds. In the case of germinating lotus seeds, it contains more active ingredients required for life phenomena as compared with pre-germination state, and thus can provide stronger efficacy.

The germinated lotus seeds can be germinated by cultivating the lotus seeds in clean water at a temperature of 20 ° C to 25 ° C. At this time, it is preferable that the lotus seeds are germinated by scratching their husks. It is preferable that the lotus seeds are cultured for 7 to 10 days after watering once or twice a day after putting them in water. It is good to place in daylight where daylight is available.

The method for producing the extract of the lotus seed used in the present invention is not particularly limited and the extract can be obtained by leaching from the lotus seed and transferring the extract to the concentrate obtained by partially or completely concentrating the leach or the concentrate again, The extracted extract and the chemical substance itself exhibiting the main effect contained in the extract.

More specifically, the extract of the lotus seeds is preferably extracted with Subcritical Water Extraction. In conventional supercritical extraction methods, the density of supercritical fluids is similar to that of liquids, and viscosity and diffusion coefficients are similar to those of gases. Therefore, when a supercritical fluid is used as an extraction solvent, it can be easily penetrated into a micro-particle of a plant and has an appropriate molecular density, so that a desired substance extract can be obtained by surrounding molecules of a desired substance and coming out. At this time, as a substance which can be used as an extraction solvent by making it into a supercritical state, it should first be non-toxic, and since the supercritical state is high temperature / high pressure, there is no danger of explosion and excellent dissolving power should be used as an extraction solvent. Once these conditions are met, any substance can be made into a supercritical fluid for extraction.

On the other hand, the subcritical technology used in the present invention is used as an extraction solvent in a state in which the solvent does not exceed the critical point. Depending on what solvent is selected, the subcritical technology can achieve the same performance as supercritical extraction. Use water. Since supercritical carbon dioxide can not extract polar substances, organic solvents should be used together as an auxiliary solvent. However, supercritical carbon dioxide can extract both nonpolar and polar substances because of its superior extraction performance and wide range of operating conditions. Do. In addition, in the case of extraction with subcritical water, a hydrolysis reaction can also proceed.

According to this extraction method, the extract of lotus seeds can be extracted in a short time using the change of dielectric constant (e) of water according to temperature and pressure in solid or semi-solid sample. Therefore, by extracting the lotus seed extract by the subcritical extraction method, the extraction speed is faster than that by the conventional solvent extraction method, and since water is used as the extraction solvent, there is no residual organic solvent or heavy metal, which is harmless to the human body and environment. On the other hand, since the process proceeds to the low-temperature process, the activity of the extract is maintained and the thermo-denatured substance can be extracted, and the process itself can easily separate and recover the extract, thereby reducing the operation cost and recycling the solvent. It is also advantageous to obtain a useful substance selectively according to extraction conditions such as temperature and pressure.

The lotus seed extract by the subcritical extraction method used in the present invention can be specifically prepared as follows. After preparing the lotus seeds, the lotus seeds are decomposed at a pressure of 50 to 200 MPa, preferably 100 MPa using a subcritical extraction device to extract an effective ingredient. The effective components are not sufficiently extracted at a pressure of less than 50 MPa and the extraction of the effective component is less than the pressure at a pressure exceeding 200 MPa. After extraction, the filter is filtered once with a filter having a pore size of 1.2 mu m, once with a filter having a pore size of 0.45 mu m, and once with a filter having a pore size of 0.2 mu m to remove impurities and microorganisms.

The composition of the present invention may contain the lotus seed extract in an amount of 0.01 to 95.0% by weight, preferably 5.0 to 95.0% by weight, more preferably 10.0 to 90.0% by weight, based on the total weight of the composition. If the content of the active ingredient is less than 0.01% by weight, it is difficult to expect the skin effect of the ingredient. If the content exceeds 95.0% by weight, the efficacy of the ingredient is not greatly increased.

The composition of the present invention may be a composition for external application to the skin, and more specifically, it may be used as a composition for external application for moisturizing skin, which can enhance the skin barrier function and induce differentiation of dermal keratinocytes. Therefore, it can be effectively used as a composition for external application for skin, which prevents or improves skin dryness or psoriasis caused by incomplete epidermal differentiation. It also has excellent effects on skin aging, skin oxidation, skin elasticity enhancement, skin wrinkle improvement, color improvement, pore contraction, skin irritation mitigation, homeostatic improvement, elasticity, acne, regeneration, sebum, waste, skin convergence, It is possible to provide an effect of improving the overall skin condition.

When the composition for external application for skin according to the present invention is formulated in the form of a cosmetic composition, it may be formulated into cosmetic formulations such as softening lotion, convergent lotion, nutritional lotion, eye cream, nutritional cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence, And the formulations thereof are not particularly limited. In addition, the composition according to the present invention can also be used as a lubricant, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, The active ingredient may be combined with any other ingredients commonly used in cosmetics, such as ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may contain adjuvants commonly used in the same cosmetic or dermatological fields. The adjuvant is introduced in an amount commonly used in the field of cosmetology or dermatology. The composition of the present invention may also contain a skin absorption promoting substance to increase the skin improving effect.

In addition, the composition according to the present invention may be a pharmaceutical composition. The pharmaceutical composition may further contain a preservative, a stabilizer, a wetting or emulsifying accelerator, a pharmaceutical adjuvant such as a salt and / or a buffer for controlling osmotic pressure, and other therapeutically useful substances.

Hereinafter, the configuration and effects of the present invention will be described in more detail with reference to examples. However, these examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Comparative Example] The extract of common lotus seed

The non-germinated lotus seeds were placed in a 70% purified water and 30% alcohol solution and left at 25 ° C for 6 hours to extract the active ingredients. After the extraction, the filter was filtered once with a filter having a diameter of 3 μm and once with a filter having a filter having a diameter of 1 μm to remove foreign matter. Finally, microorganisms were removed with a filter to obtain a general lotus seed extract.

[Example 1] Germination lotus seed extract

The germinated lotus seeds were placed in a 70% pure water and 30% alcohol solution and left at 25 ° C for 6 hours to extract the active ingredients. After extraction, the filter was filtered once with a filter having a diameter of 3 μm and once with a filter having a filter having a diameter of 1 μm to remove foreign matter. Finally, microorganisms were removed with a filter to remove germination lotus seeds.

[Example 2] A subcritical lotus seed extract

The active ingredient was extracted for 4 hours at 40 캜 at 20 MPa using a subcritical extraction device, not in germination state. After extraction, the filter was filtered once with a filter having a pore size of 1.2 mu m, once with a filter having a pore size of 0.45 mu m, and once with a filter having a pore size of 0.2 mu m to remove impurities and microorganisms.

[Example 3] Gout subcritical lotus seed extract

The germinated lotus seeds were extracted for 4 hours at 40 ° C and 20 MPa using a subcritical extraction device. After extraction, the filter was filtered once with a filter having a 1.2 μm gap, once with a filter having a 0.45 μm gap and once with a filter having a 0.2 μm gap to remove germs and microorganisms and obtain germination supernatant lotus seed extract.

[Test Example 1] Measurement of skin moisture persistence

In order to measure the amount of skin moisture change and the sustainability at the time of one application, the water increase amount of each of Production Examples 1 to 3 and Comparative Production Examples 1 to 2 prepared by the composition of the following Table 1 was evaluated by using a corneometer.

ingredient Production Example 1 Production Example 2 Production Example 3 Comparative Preparation Example 1 Comparative Production Example 2 Comparative Example (lotus seed extract) - - - - 90 Example 1 (germination lotus seed extract) 90 - - - - Example 2 (Subcritical lotus seed extract) - 90 - - - Example 3 (germination supernatant lotus seed extract) - - 90 - - Purified water 0 0 0 90 0 Butylene glycol 3 3 3 3 3 glycerin 3 3 3 3 3 Hyaluronic acid 2 2 2 2 2 Betaklukan One One One One One Pentylene glycol One One One One One

(Unit: wt%)

First, 25 women aged 30 years or older were selected and washed with running water in flowing water. The test area was marked with a circle 1.5 cm in diameter at a distance of 5 cm from the wrist. The moisture content of the test area was measured by the following method using a moisture analyzer (CM825, Courage & Khazaka, Germany) after waiting for 30 minutes in a constant temperature and humidity chamber maintained at 23 ± 1 ° C and 45 ± 5% RH.

<Measurement method of moisture meter>

The amount of water present in the epidermis of the skin was measured by measuring the degree of ion of water using a sensor, numerically calculating the amount of moisture, and then measuring the moisture content.

1. A probe of a corneometer is placed on the skin area to be measured.

2. When the probe is pressed against the skin, the skin's electrical conductivity is quantified and displayed on the screen.

3. Measurement was repeated with different measurement sites.

4. After one measurement, the sensor was wiped with a tissue such as a kim wipe and then re-measured.

Thereafter, the same amount of each of Preparation Examples 1 to 3 and Comparative Production Examples 1 and 2 was applied to both test strips at the test site and absorbed. After 30 minutes, 1 hour, 2 hours, 4 hours and 6 hours elapsed in the constant temperature- The moisture content of the test site was measured. The water content increase rate is a calculated value relative to the state before application of Preparation Examples 1 to 3 and Comparative Production Examples 1 and 2, and Table 2 and Fig. 1 show average results.

Before application 30 minutes 1 hours 2 hours 4 hours 6 hours Control group
(Mud) 26.2 26.11 26.21 26.15 26.19 26.17 Production Example 1 26.34 51.99 44.63 43.39 41.13 40.58 Production Example 2 26.12 51.85 44.72 43.27 41.59 39.98 Production Example 3 26.08 53.77 50.48 49.64 46.21 45.69 Comparative Preparation Example 1 26.05 48.13 43.17 39.53 38.52 33.63 Comparative Production Example 2 25.92 49.95 43.54 42.87 40.25 36.14

As can be seen from Table 2 and FIG. 1, when the preparations 1 to 3 containing the lotus seed extract were applied, it was found that the higher moisture content in the skin lasted for a longer period of time than when the prescription of Comparative Preparation Example 1 was applied once . In addition, in the case of Production Examples 1 to 3 of the present invention, the water content lasted longer as compared with Comparative Production Example 2 in which the common lotus seed extract was applied. In particular, in the case of Production Example 3 using germicidal lotus seed extract, it was confirmed that as shown in Fig. 1, superior skin moisture persistence was obtained above the sum of the effects of Production Example 1 and Production Example 2.

[Test Example 2] Measurement of skin moisturizing effect

TEWL (Trans Epidermal Water Loss) was measured to measure long-term skin moisturizing effect. At this time, the tests were carried out using the same Production Examples 1 to 3 and Comparative Production Examples 1 to 2 as in Test Example 1.

First, 30 women aged 30 years or older were allowed to apply Production Examples 1 to 3 and Comparative Production Examples 1 and 2 twice a day for 4 weeks on both cheeks, 2 weeks after application, 4 weeks after application, TEWL (Trans Epidermal Water Loss) was measured by the following method at constant temperature and humidity (24 ° C, 40% relative humidity).

<TEWL measurement method>

The amount of moisture evaporating from the skin was calculated according to the area and time, and the amount of water evaporated was read and quantified with the temperature and humidity electronic sensor to measure the moisture of the skin.

1. Environmental conditions: The temperature and humidity were kept constant and only the minimum number of persons other than the measurer and the subject were left. At this time, the air flow was blocked as much as possible.

2. Place the TEWL probe on the subject's measurement site.

3. When placing the probe on the measurement site, place it lightly so that excessive force is not applied to the skin to leave marks on the skin, and make an angle of 90 degrees with the ground.

4. The value at which the TEWL value of the skin becomes constant as time passes is measured for about 1 to 3 minutes.

5. Handle the probe as far as possible so that the temperature of the measurer is not transmitted.

6. The amount of water evaporated per hour per area (g / m ² / h) was calculated.

The moisture resistance was compared with the measured TEWL, and the results are shown in Fig. As can be seen from FIG. 2, in the case of Production Examples 1 to 3, it can be seen that the evaporation amount of moisture was significantly reduced as compared with Comparative Production Examples 1 and 2. In Comparative Preparation Example 2 using common lotus seed extract 2 weeks later, the skin moisture content was reduced to about 70%, but in Production Examples 1 to 3 of the present invention, 80% or more was maintained. In particular, in the case of Preparation Example 3 using germicidal extract of lotus seeds, the skin moisture was maintained at about 85% even after 4 weeks, and thus it was found that the skin moisturizing ability was excellent.

[Test Example 3] Evaluation of subjective efficacy

At the end of the above Test Examples 1 and 2, a questionnaire was prepared to determine how much subjective skin dryness was improved. The questionnaire was designed to answer questions about skin tightness, itching, skin texture, and overall improvement, and scored on scales from 1 to 10 for Manufacturing Examples 1 to 3 and Comparative Manufacturing Examples 1 and 2.

Better skin tightening Itching improvement Skin texture improvement Comprehensive Improvement Production Example 1 7 6 7 7 Production Example 2 7 6 7 7 Production Example 3 8 8 9 8 Comparative Preparation Example 1 5 3 4 3 Comparative Production Example 2 5 5 6 5

As shown in Table 3, the subjects replied that excellent improvement was observed in all areas including skin pulling, itching, skin texture, and the like in the areas to which Manufacturing Examples 1 to 3 were applied. However, all the items of Comparative Production Examples 1 and 2 were not greatly improved.

Claims (6)

A cosmetic composition for improving skin moisturizing power containing germinated lotus seed extract. A cosmetic composition for improving skin moisturizing power comprising lotus seed extract extracted by subcritical extraction method. The cosmetic composition according to claim 1, wherein the germinated lotus seed extract is extracted by subcritical extraction. 4. The cosmetic composition according to any one of claims 1 to 3, wherein the lotus seed extract contains gamma-linolenic acid. 4. The cosmetic composition according to any one of claims 1 to 3, wherein the lotus seed extract is contained in an amount of 10 to 90% by weight based on the total weight of the composition. delete

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