KR102342047B1 - Cosmetic composition with skin moisturizing and elasticity that contains vaginal extract as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition having the effects of moisturizing skin and strengthening skin barrier, containing a Plantago asiatica extract as an active ingredient. The present invention aims to exhibit excellent efficacy in skin moisturization and skin barriers by mixing the Plantago asiatica extract, a Foeniculum vulgare fruit extract, lactobacillus plantarum culture, an Artemisia princeps leaf extract, and polyglutamic acid together. In addition, various development possibilities as a new cosmetic raw material can be raised by applying the same to the actual human skin.

Description

Cosmetic composition with skin moisturizing and elasticity that contains vaginal extract as active ingredient}

The present invention relates to a cosmetic composition containing plantain extract as an active ingredient and having skin moisturizing and elasticity and skin barrier strengthening effects.

As time goes by, the secretion of various hormones that internally control the metabolism of human skin decreases, and the function and activity of immune cells decreases, thereby reducing the biosynthesis of immune proteins and biological constituent proteins necessary for the living body. . In addition, externally, due to the destruction of the ozone layer, the content of ultraviolet rays reaching the surface of the sun increases, and as environmental pollution further deepens, the thickness of the skin decreases, wrinkles increase, elasticity decreases, and freckles , freckles and age spots also increase, causing various changes. However, since modern society places importance on young and beautiful appearance, there is a great desire to solve such skin problems. Accordingly, various wrinkle improving agents, elasticity improving agents, whitening agents, and the like have been developed.

On the other hand, symptoms such as wrinkles, loss of elasticity, and dry skin appearing on the skin change or decrease the content and arrangement of collagen, elastin, hyaluronic acid, proteoglycan, glycosaminoglycan, fibronectin and glycoprotein, which are substances constituting the skin. , the ability to bind with water is reduced, and as a result, the matrix material constituting the skin is reduced, and the skin's ability to contain water is lost. Therefore, it is important for skin health to maintain normal skin moisture to solve this problem.

On the other hand, tumor necrosis factor alpha (TNFα, tumor necrosis factor α), interleukin-1beta (IL-1β, interleukin- 1β), an enzyme that produces prostaglandin, cyclooxygenase-2 (Cox-2, cyclooxygenase), increases biosynthesis, and matrix metalloprotease (MMP, an enzyme that decomposes skin tissue by these inflammatory factors) It is known to increase the biosynthesis of matrix metalloproteinase). Therefore, a substance capable of thickening the skin by proliferating the skin cells or increasing the matrix material constituting the skin, as described above, of type I collagenase (MMP-1) that decomposes collagen, a matrix material constituting the skin. A substance capable of inhibiting biosynthesis, a substance capable of inhibiting the biosynthesis of cyclooxygenase-2, a substance capable of inhibiting the biosynthesis of tumor necrosis factor, a substance that promotes the production of tropoelastin and fibrillin in skin fibroblasts If the material is developed, symptoms of the skin such as wrinkles, loss of elasticity, and dry skin can be alleviated. Therefore, it is necessary to develop a cosmetic with new raw materials that will contribute to strengthening the skin barrier by controlling the thickness of the skin, maintaining an elastic and healthy skin condition, and keeping the moisture of the skin moist.

Korean Patent Laid-Open No. 10-2009-0025500 (published on March 11, 2009) relates to a cosmetic composition for skin protection against ultraviolet rays containing plantain extract, wherein the plantain extract absorbs ultraviolet rays and prevents the transformation of skin cells by ultraviolet rays. It has an effect that relieves skin irritation and does not appear, and it is described for increasing the UV protection effect and restoring the skin damaged by UV rays.

The present invention provides skin hydration and elasticity by mixing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract, and polyglutamic acid together. An object of the present invention is to provide a cosmetic composition that exhibits excellent efficacy in strengthening the skin barrier.

The present invention provides a cosmetic composition for moisturizing the skin containing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma-glutamic acid as active ingredients.

On the other hand, in the cosmetic composition for moisturizing the skin of the present invention, the plantain extract is preferably selected from among methanol, n-hexane, methylene chloride, and ethyl acetate in plantain. It is preferable to extract using one or more as an extraction solvent.

In addition, the present invention provides a cosmetic composition for enhancing skin elasticity containing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma-glutamic acid as active ingredients.

On the other hand, in the cosmetic composition for enhancing skin elasticity of the present invention, the plantain extract is preferably selected from among methanol, n-hexane, methylene chloride, and ethyl acetate in plantain. It is preferable to use any one or more as an extraction solvent for extraction.

In addition, the present invention provides a cosmetic composition for strengthening the skin barrier containing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma-glutamic acid as active ingredients.

On the other hand, in the cosmetic composition for strengthening the skin barrier of the present invention, the plantain extract is preferably selected from among methanol, n-hexane, methylene chloride, and ethyl acetate in plantain. It is preferable to use any one or more as an extraction solvent for extraction.

The present invention is a plantain extract and fennel fruit extract, Lactobacillus plantarum By mixing the culture, mugwort leaf extract, and polyglutamic acid together, it is intended to exert excellent effects on skin moisture, elasticity, and skin barrier. In addition, by applying it to actual human skin, various development possibilities as a new cosmetic raw material can be raised.

1 is a schematic diagram showing the separation process of Lactobacillus plantarum of the present invention.
2 is a graph showing the results of measuring the cell viability of the present invention plantain biome-13 and its fractions.
3 is a graph showing changes in mRNA (a) and protein (b) expression of Involucrin, Loricrin, and Filaggrin by the present invention plantain biome-13 and its fractions. .
Figure 4 is a result showing the expression change of human β-defeinsin (HBD-1, HBD-2, HBD-3) and cathelicidin (cathelicidin, LL-37) mRNA by the present invention plantain biome-13 and its fractions; It is a graph.
5 is a result showing the production amount of hyaluronic acid (a) and mRNA expression change (b) of hyaluronic acid synthetase (HAS-1, HAS-2, HAS-3) by the plantain biome-13 and its fractions of the present invention; It is a graph.
6 is a graph showing the result of measuring the change in moisture content according to time (20 minutes, 2 hours) after applying the plantain biome-13 of the present invention to actual human skin. It corresponds to the test substance written on the left in order from top to bottom of the graph.
7 is a graph showing the results of skin moisture content (a) and improvement rate (b) after application of an actual skin clinical test with a cosmetic composition containing plantain biome-13 of the present invention.
8 is a graph showing the results of measuring the elasticity of the skin over time by performing an actual skin clinical trial with a cosmetic composition containing plantain biome-13 of the present invention.

The present invention provides a cosmetic composition for moisturizing the skin containing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma-glutamic acid as active ingredients. In addition, the present invention provides a cosmetic composition for enhancing skin elasticity containing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma-glutamic acid as active ingredients. In addition, the present invention provides a cosmetic composition for strengthening the skin barrier containing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma-glutamic acid as active ingredients.

Plantain is a herbaceous perennial belonging to the family Plantaginaceae, and its young leaves are edible. In oriental medicine, the roots are pulled and dried, and the seeds are used as medicinal herbs, and are distributed in Korea, Japan, Sakhalin, Taiwan, China, Siberia, Eastern Himalayas, Java, and Malaysia. . Since plantain contains glycosides called "plantaginin" and tannins, it is used as a diuretic, antitussive, expectorant, gastric, antidiarrheal, antipyretic, anti-inflammatory, and tonic. , is also effective in seawater from waste heat. It also has antibacterial action, so it is also used in the treatment of acute and chronic bacterial diarrhea, nephritis, cystitis, and urethritis. As a folk medicine, it is said that it is good to soften raw leaves by exposing them to the fire for toothache. For toothache, rubbing raw leaves with salt and biting them on a sore tooth is said to stop the toothache.

The phenylpropanoid component of plantain seeds is known to have diuretic and anti-inflammatory effects (especially hepatitis), and phytosterol glyicoside, mucilage ), it is used for diet and constipation improvement. In addition, immune enhancing action, antiviral action, and plantagoside that activate complement are α-mannosidase inhibitors and have immunosuppressive functions. Also, plantain leaves have wound healing effects, and phenylethanoids also reduce swelling in the ears of mice. Ursolic acid and fatty acids contained in plantain are known to act as cyclooxygenase-2 (COX-2) inhibitors.

The present invention is to improve the overall symptoms of skin wrinkles, elasticity, moisturizing, etc. by using plantain extract, and furthermore, through combination with other substances, a cosmetic composition having excellent efficacy in skin moisturizing, elasticity, and skin barrier strengthening. We want to provide new raw materials.

Meanwhile, in the present invention, the plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma glutamic acid are preferably 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5 : It is preferable to mix in a ratio of 0.5 to 1.5, more preferably 1:1: 1: 1: 1 is good to mix in a ratio.

Fennel ( Foeniculum vulgare , fennel) is a plant native to the Mediterranean coast with a height of 1.5 to 2 m. It has a straight stem, an empty inside, and a long sheath-shaped leaf with a sharp tip, and is divided into 3 or 4 branches like a feather. have It is mainly used as a food or spice and has various pharmacological effects such as treatment of various female diseases, relieving stress, good sleep, and promoting diuresis. In the present invention, fennel fruit extract was used.

Lactobacillus bacteria (Lactobacillus) are often used through fermentation by the fermentation of sugars obtained using the same energy as a bacterium to generate a large amount of lactic acid to make the fermentation of kimchi, yogurt, cheese and the like. In the present invention, a Lactobacillus plantarum culture was used, and at this time, the Lactobacillus is not limited to any known in the art, but preferably Lactobacillus plantarum isolated from yogurt. In the present invention, Lactobacillus plantarum separated from the separation process of FIG. 1 was used.

Mugwort is one of the perennial plants belonging to the Asteraceae family, and the whole is covered with cobweb-like hairs and grows with underground stems extending sideways. Mainly, young shoots are made into greens or rice cakes, and mature ones are used medicinally for moxibustion, etc., and the scent of mugwort is also used to repel mosquitoes and flies. In addition, it has various pharmacological effects such as prevention of hypertension and arteriosclerosis, immune function and detoxification, improvement of liver function and anti-aging, treatment of various gynecological diseases, and improvement of gastrointestinal disorders.

Poly-gamma-glutamic acid (PGA) is a polymer in which DL-glutamic acid is combined with gamma-glutamyl. It is produced from a strain of Bacillus sp. isolated from natto and Kinema of Nepal. Polygamma glutamic acid is an edible, water-soluble and biodegradable polymer material, and is used as a raw material for cosmetics as a desiccant and moisturizer.

On the other hand, the extracts of the present invention are not limited to any extraction method known in the art, and may be prepared using a conventional solvent under conditions of ordinary temperature and pressure, but for example (a) water, carbon number 1 -4 anhydrous or hydrous lower alcohols (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, The extraction is performed using a solvent extraction method using a solvent selected from the group consisting of chloroform, hexane, and mixtures thereof, (b) supercritical extraction using reduced pressure and high temperature with carbon dioxide, or (c) ultrasonic extraction. In the present invention, it is preferable to extract using at least one selected from methanol, n-hexane, methylene chloride, and ethyl acetate as an extraction solvent.

On the other hand, according to the following experiment, 'Plantain Biome-13', which is a mixture of plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract, and polyglutamic acid of the present invention, is safe because it does not show cytotoxicity. In addition, it is possible to strengthen the physical barrier of the skin by increasing the expression of genes secreted when keratinocyte differentiation is promoted, and furthermore, by increasing the expression of antibacterial peptides, the chemical barrier of the skin can be strengthened. In addition, it was excellent in moisturizing the skin by increasing the amount of hyaluronic acid production and increasing the expression of hyaluronic acid synthase, which was confirmed through the improvement of skin moisture level even when applied to actual human skin. In addition, it was confirmed that the degree of improvement in moisture content was excellent when the five substances were mixed rather than each of the extracts treated alone. In addition, as a result of a clinical test on a cosmetic composition containing plantain biome-13, it was confirmed that the improvement of skin elasticity was excellent. Taken together, the 'plantain biome-13' of the present invention was confirmed as a new raw material for a cosmetic composition having excellent skin moisturizing, elasticity, and skin barrier strengthening effects by using a mixture of five substances.

On the other hand, the cosmetic composition of the present invention, for example, solutions, suspensions, emulsions, pastes, lotions, gels, water-soluble liquids, creams, essences, surfactant-containing cleansing, oils, oil-in-water (O/W) type and water-in-oil (W / O) any one of the basic cosmetic formulation selected from the type; skin; Lotion; eye cream; soothing gel; Ointment; Formulations for mask packs; formulation for body wash; peeling gel; Oil-in-water and water-in-oil makeup base; foundation; skin cover; Any one color cosmetic formulation selected from lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color and eyebrow pencil; formulations for the scalp; It may be any one selected from among.

In addition, the cosmetic composition of the present invention may contain auxiliary agents such as hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blocking agents, pigments, odorants, dyes, etc. commonly used in the art to which the present invention pertains. can The amount of these various adjuvants is an amount conventionally used in the art, for example, 0.001 to 30% by weight relative to the total weight of the composition. However, in any case, the auxiliary agent and its ratio will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

On the other hand, in the cosmetic composition of the present invention, when the formulation is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Benzoate, propylene glycol, 1,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

In addition, in the cosmetic composition of the present invention, when the formulation is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester Suspending agents such as, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth may be used.

In addition, in the cosmetic composition of the present invention, when the formulation is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica as a carrier component , talc or zinc oxide may be used.

In addition, in the cosmetic composition of the present invention, when the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as carrier performance, and in particular, in the case of a spray, It may additionally contain propellants such as chlorofluorohydrocarbons, propane/butane or dimethyl ether.

In addition, in the cosmetic composition of the present invention, when the formulation is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

In addition, the cosmetic composition of the present invention can be used overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

Hereinafter, the present invention will be described in more detail in the following Examples and Experimental Examples. However, the scope of the present invention is not limited only to the following examples and experimental examples, and includes all modifications of the technical idea equivalent thereto.

[Examples 1 to 6: Preparation of plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract, polyglutamic acid and mixtures thereof]

Dried plantain powder 200g, fennel fruit powder 200g, Lactobacillus plantarum culture powder 200g, mugwort leaf powder 200g, and polygamma glutamic acid powder 200g 80% methanol 900ml was added, extracted for 3 hours, and the extract was After filtering, it was concentrated with a rotary vacuum evaporator and then freeze-dried to obtain 92.5 g of methanol extract (M), which was named 'Plantary Biome-13 (Jilgyungyi Biome-13)' (Example 6). The Lactobacillus culture powder was used from the powdered Lactobacillus plantarum strain culture solution separated by the separation process of FIG. 1 .

The plantain biome-13 ((methanol extract, M) is n-hexane (n-hexane; H), methylene chloride (MC), ethyl acetate (ethyl acetate; E), n-butanol using a polarity difference (n-butanol; B) was fractionated twice in each step by adding a solvent, 90 g of the methanol extract was suspended in water, fractionated by mixing with the same amount of n-hexane, and the water layer was again fractionated with methylene chloride Then, the water layer was again fractionated with ethyl acetate, and the remaining water layer was finally fractionated with n-butanol.After that, each fraction layer obtained was filtered, concentrated and lyophilized to obtain a fraction H of 47.8 g and a fraction MC of 1.4. g, 1.6 g of fraction E, and 5.5 g of fraction B. The sample obtained after freeze-drying was used in the experiment while being stored in a deep freezer at -78°C.

In addition, each of fennel fruit powder, plantain powder, Lactobacillus plantarum culture powder, mugwort leaf powder, and polygamma glutamic acid powder was extracted in the same manner as the above methanol extraction to extract plantain extract (Example 1, plantain powder 1). %), fennel fruit extract (Example 2, fennel fruit powder containing 1%), Lactobacillus plantarum culture (Example 3, lactobacillus fermented powder containing 1%), mugwort leaf extract (Example 4, mugwort leaf) Powder containing 1%) and polyglutamic acid (Example 5, containing 1% polygamma glutamic acid powder) were prepared. Thereafter, fractions were obtained from each methanol extract as described above.

[Experimental Example 1: Evaluation of cytotoxicity of the plantain biome-13 of the present invention]

In this experimental example, the cytotoxicity of the plantain biome-13 prepared in Example 6 was evaluated.

HaCaT cells, which are human keratinocytes, were treated with plantain biome-13 and its fractions at a concentration of 0, 10, 50, or 100 μg/ml, and then XTT assay was performed to measure cell viability. As a result, as shown in Figure 2, the plantain biome-13 (M) and all fractions showed a cell viability of 95% up to a concentration of 50 ㎍ / ㎖. However, when the plantain biome-13 (M) was treated at a concentration of 100 μg/ml, the survival rate was 99%, 87% for fraction H, 28% for fraction MC, 77% for fraction E, and 84% for fraction B. , it was confirmed that plantain induced cytotoxicity in the case of the remaining fractions except for biome-13 (M). Therefore, the following experiments were performed by determining a concentration of 50 μg/ml that did not show cytotoxicity in all treatment groups.

[Experimental Example 2: Evaluation of skin barrier strengthening efficacy of plantain biome-13 of the present invention]

In this experimental example, the skin barrier efficacy of the plantain biome-13 prepared in Example 6 was evaluated.

1) Efficacy evaluation of plantain biome-13 on the physical skin barrier

The skin barrier maintains the normal function of the physical barrier by forming the outer membrane of the keratinocytes by differentiation promoting factors secreted during the final stage of differentiation of keratinocytes. In order to investigate the effect of plantain biome-13 on the skin barrier function, the expression change of the genes secreted when promoting the differentiation of keratinocytes after treatment with keratinocytes was confirmed. Specifically, HaCaT cells, a human-derived keratinocyte line, were treated with plantain biome-13 and its fractions at a concentration of 50 μg/ml, respectively, and cultured for 24 hours, followed by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Expression changes in mRNA and protein of involucrin, loricrin, and filaggrin were observed through western blotting. In this case, as a positive control group, calcium, which is involved in strengthening the skin barrier function as a bio factor involved in promoting the differentiation of keratinocytes, was used.

As a result of RT-PCR, as shown in Fig. 3a, in the case of involucrin gene, in the group treated with plantain biome-13 (M) and its fractions (H, MC, E, B) compared to the control group, both mRNA The expression level was increased, and, in particular, it was found to be significantly increased in M, H, E, and B. On the other hand, in the case of loricrin and filaggrin genes, mRNA expression was not significantly increased by the composition treatment. Furthermore, as a result of observation of changes in protein expression through western blotting, as shown in FIG. 3b , in the case of the involucrin gene, the protein expression levels in E and B were increased compared to the control group, and in the case of the filaggrin gene, the overall protein expression level increased. did

2) Efficacy evaluation of plantain biome-13 on chemical skin barrier

HaCaT cells, a human-derived keratinocyte line, were treated with plantain biome-13 and its fractions at a concentration of 50 μg/ml, respectively, and cultured for 24 hours. HBD-2, HBD-3) and cathelicidin (LL-37) mRNA expression changes were observed.

As a result, as shown in FIG. 4 , in the case of HBD-1, the mRNA expression level was increased in all treatment groups compared to the control group. In addition, in the case of LL-37, it was found that the mRNA expression level tended to increase in all treatment groups compared to the control group. As such, it was confirmed that the plantain biome-13 can strengthen the skin physical and chemical barriers.

[Experimental Example 3: Evaluation of skin moisturizing effect of plantain biome-13 of the present invention]

In this experimental example, the skin moisturizing effect of the plantain biome-13 prepared in Example 6 was evaluated.

HaCaT cells, a human-derived keratinocyte line, were treated with plantain biome-13 and its fractions at a concentration of 50 μg/ml, respectively, and cultured for 24 hours. acid) was measured. Thereafter, RT-PCR was performed to observe changes in the expression of intracellular hyaluronic acid synthase (HAS), HAS-1, HAS-2, and HAS-3 mRNA.

As a result, as shown in Figure 5a, the hyaluronic acid concentration was increased in all treatment groups compared to the control group. In addition, as shown in FIG. 5b , in the case of HAS-1, the mRNA expression level was increased in all groups compared to the control group, and in the case of HAS-3, it was confirmed that there was a tendency to increase in M, H, E, and B. As such, the plantain biome-13 was confirmed to be effective in moisturizing the skin.

[Experimental Example 4: Evaluation of skin moisturizing effect of plantain biome-13 on human skin]

In this experimental example, it was attempted to evaluate the skin moisturizing effect of the plantain biome-13 prepared in Example 6 on actual human skin.

Composition containing the fennel fruit extract, plantain extract, Lactobacillus plantarum culture, mugwort leaf extract, and polyglutamic acid of Examples 1 to 5, respectively, on the normal skin of the arm in the human body, the plantain biome of Example 6- After the composition containing 13 or the control composition not containing all of the above five extracts was applied once, the moisture level was increased or decreased by using a moisture meter (Corneometer TM) for 0 minutes (before application), 20 minutes after application, and Each measurement was performed 2 hours after application (n=9). The composition was applied equally to a square of 1.5 cm × 1.5 cm using a pipette for a viscous material in 15 μl each. The limit value was graphically shown by correcting the increase in the area to which the control composition was applied.

As a result, as shown in FIG. 6 , in the case of plantain biome-13 (described as 'plantain extract 5% 5-MIX'), the moisture content measured 20 minutes after application was significantly increased compared to the site where the control composition was applied. and (n=9), showed a significant increase in moisture content up to 2 hours. On the other hand, each composition containing fennel fruit extract, plantain extract, polyglutamic acid, Lactobacillus plantarum culture, and mugwort leaf extract alone showed relatively insignificant effects of increasing moisture. Therefore, when applied to the skin, it was confirmed that the mixing of the above five extracts had a synergistic effect so that the moisturizing effect could be maintained for a longer period of time.

[Example 7: Preparation of cosmetic composition containing plantain biome-13 of the present invention and skin clinical test using the same]

In this example, a cosmetic composition of a gel formulation containing the plantain biome-13 prepared in Example 6 was prepared, and a skin clinical test was performed using it.

1) Preparation of gel-type cosmetic composition containing plantain biome-13

First, disodium EDTA and plantain biome-13 of the present invention were put in purified water and dissolved using an azimuth mixer. Next, xanthan gum (Xanthangum) was gradually added to the mixture to increase the thickness, and then, coco-betaine and 1,2-hexanediol were added and mixed. The detailed composition is shown in Table 1 below.

Furtherance Content (parts by weight) control Example 7-1 Example 7-2 Example 7-3 Purified water to 100 to 100 to 100 to 100 xanthan gum 1.0 1.0 1.0 1.0 Disodium EDTA 0.02 0.02 0.02 0.02 Plantain Biome-13 0 0.01 5 10 coco-betaine 20 20 20 20 1,2-Hexanediol 2 2 2 2

2) Skin clinical test to confirm skin moisturizing effect

(1) Equipment used

'Corneometer CM 825 (Courage & Khazaka, Germany)' was used, and the Corneometer is a device that indirectly measures the moisture content by using the property that the capacitance of the stratum corneum increases in proportion to the moisture content. Due to its high reproducibility, easy handling, and short measurement time, it is the most widely used instrument in the world to measure the amount of moisture in the stratum corneum.

The measurement principle is based on a physical law using two complex metal plate capacitors that are electrically insulated by a medium that is an insulator. The amount of electricity that stores electric charge is called capacitance, and the capacitance of the skin tends to be proportional to the moisture content. . The measurement unit is expressed as Arbiturary Unit (A.U.), which can be thought of as a relative, arbitrary number of skin moisture content. The measurement method is to measure the moisture content by pressing the probe attached to the corneometer vertically to the skin surface and then lightly pressing it.

(2) Test sequence

After washing the face using the standard cleanser provided by the tester, tap lightly with a paper towel to remove moisture, and then leave it under constant temperature and humidity conditions (20~24℃, 40~60%RH) for 30 minutes. At this time, the measurement site - the point where the horizontal line on the alare and the mid-pupillary line meet - was partitioned and the instrument measurement was performed so that the measurement could be made at the same site for each evaluation. The corneometer was measured 5 times, and the average value of the measured values was recorded as the measured value.

After applying the test sample to the test site and waiting in constant temperature and humidity conditions (20~24℃, 40~60%RH) for 30 minutes, device evaluation was performed at the same site in the same manner as after the control application. A dermatologist confirmed the presence of adverse reactions (irritating symptoms such as pruritus and erythema). The test participation fee was paid to the subjects immediately after the end of the test.

(3) Evaluation method

- Evaluation of water content improvement effect using Corneometer

After applying the control to the test site and after applying Examples 7-1 to 7-3, the change in skin moisture content was measured using a Corneometer, and the improvement rate was confirmed as shown in Equation 1 below.

- Stability evaluation by a dermatologist

The occurrence of side effects (erythema, edema, scale, itching, stinging, burning, stiffness, tingling and other abnormalities) due to the use of the sample during the test period was evaluated.

(4) Statistical analysis method

Significance was confirmed using the Minitab 19 (Minitab® 19.2, Minitab Inc.) program. When the result value is estimated to have a normal distribution through the Ryan-Joiner Normality Test, the significance was confirmed through the following parametric statistical method.

In the case of comparison of pre- and post-result values within the same group, paired t-test is used for comparison of measured values before and after the test. Significance was confirmed at the <0.05 level. For comparison of results between two or more different groups, Welch's t-test is used to compare results between two groups, and for comparison of results between groups of three or more, significance level p < Significance was confirmed at the 0.05 level. In the case of comparing the results of two or more different groups repeatedly measured, the significance is checked at the significance level p < 0.05 through repeated measure ANOVA. The difference in the results between groups was confirmed through Analysis of Covariance with .

On the other hand, when normality was rejected in the Ryan-Joiner Normality Test, significance was confirmed through the following non-parametric statistical method. In the case of comparison of pre- and post-result values within the same group, Wilcoxon signed rank test is used to compare values measured before and after the test. did For comparison of results between two or more different groups, the Mann-Whitney U test is used to compare the results between the two groups, and the Kruskal-Wallis test to compare the results between groups of 3 or more is significant at the significance level p < 0.05. was confirmed. In the case of comparing the results of two or more different groups repeatedly measured, the difference in the results between the groups was confirmed at the significance level p < 0.05 through Friedman's test. All data were summarized as mean and standard deviation for continuous variables and frequency and percentage for categorical variables.

(5) Subject information and test results

- Subject basic information

NO. ID Gender Age NO. ID Gender Age One 2171 Female 24 12 2771 Female 45 2 3257 Female 25 13 976 Female 46 3 3109 Female 26 14 2095 Female 47 4 2164 Female 27 15 3325 Female 48 5 4226 Female 38 16 3258 Female 52 6 3526 Female 38 17 3525 Female 53 7 3705 Female 38 18 3922 Female 54 8 2927 Female 39 19 2450 Female 55 9 3552 Female 39 20 2529 Female 55 10 3881 Female 39 21 2031 Female 57 11 3678 Female 41

total number of subjects 21 people gender Male: 0 / Female: 21 average age 42 years old age distribution 20's 4 people 30's 6 people 40's 5 people 50's 6 people

- Test result

Measuring result of stratum corneum moisture content (Comeometer Value, A.U.) NO. test site After application of control Example 7-1 After application Example 7-2 After application Example 7-3 After application One 61.4 61.5 61.8 62.2 2 56.0 56.1 56.4 67.8 3 26.2 26.3 26.6 28.4 4 40.6 40.7 42.0 46.0 5 25.4 25.5 35.2 59.2 6 32.0 32.1 38.1 41.4 7 27.8 27.9 35.7 67.6 8 47.8 47.9 50.1 56.8 9 27.4 27.5 29.1 47.2 10 33.8 33.9 40.8 45.0 11 46.4 46.5 48.6 55.0 12 42.4 42.5 48.3 56.2 13 52.8 52.9 53.7 56.0 14 19.4 19.5 47.0 66.0 15 32.2 32.3 34.1 39.0 16 60.2 60.3 62.0 72.0 17 34.2 34.3 39.0 56.2 18 38.0 8.1 41.5 52.8 19 38.4 38.5 46.3 63.6 20 46.0 46.1 47.2 55.0 21 45.6 45.7 48.0 50.2

Moisture content in the stratum corneum (Comeometer Value) normality test, Ryan-Joiner Normality Test division average N RJ p -value black exam
part After application of control 39.71 21 0.989 >0.100 normal distribution Example 7-1 After application 39.81 21 0.988 >0.100 normal distribution Example 7-3 After application 54.46 21 0.984 >0.100 normal distribution

Statistical comparison before and after stratum corneum moisture content (Comeometer Value), paired t test division number of observations Change in stratum corneum moisture content T-value p -value average Standard Deviation test site 21 -14.74 12.44 -5.43 <0.001

Summary of stratum corneum moisture content (Comeometer Value) results division Measured value (A.U.) improvement rate p -value After application of control Example 7-3 After application test site 39.71 54.46*** 37.12% <0.001

-significant probability : *p<0.05, **p<0.01, ***p<0.001

Skin abnormalities that occurred during the trial period division erythema edema scalding itch sting burning sensation stiffness sting
stutter Etc
dystrophy mild 0 0 0 0 0 0 0 0 0 moderate 0 0 0 0 0 0 0 0 0 severe 0 0 0 0 0 0 0 0 0

The test results were as shown in Tables 4 to 6, and as shown in Table 7 and Figure 7, which summarized the results, in the case of Example 7-3 (one-time application of the test sample), the moisture content of the stratum corneum was statistically higher than that of the control area. was increased to a significant level (p<0.05), confirming that the test sample had an immediate skin moisture content improvement effect. In addition, as shown in Table 8, it was confirmed that no special abnormal symptoms occurred during the test period.

(6) Considerations and Conclusions

Immediate skin moisture content of Ecoment's "Plantary Biome-13" sample (including fennel fruit extract, plantain extract, Lactobacillus plantarum culture, mugwort leaf extract, and polyglutamic acid) for a total of 21 subjects An improvement efficacy evaluation test was conducted, and all 21 subjects completed the test without dropouts.

The test results were as follows. 1) The Corneometer measurement value (A.U.) indicating the skin moisture content increased by 37.12% after application of the Example 7-3 sample compared to the control sample, indicating that the stratum corneum moisture content of the test site was improved. 2) No special adverse reactions were observed in all subjects during the test period.

In conclusion, the test site where the Ecoment Co., Ltd. “Plantary Biome-13” test sample was applied once showed a statistically significant (p<0.05) increase in the moisture content of the stratum corneum compared to before application, so that the test sample instantly moisturized the skin. It was judged to have the effect of improving the content. In addition, no special adverse reaction was observed in all subjects, so it was considered a safe sample. (temporary, there are individual differences)

[Example 8: Preparation of cosmetic composition containing plantain biome-13 of the present invention and skin clinical test using the same]

In this example, a cosmetic composition of a cream formulation containing the plantain biome-13 prepared in Example 6 was prepared, and a skin clinical test was performed using it.

1) Preparation of a cream-type cosmetic composition containing plantain biome-13 (5-Mix)

A cosmetic composition of a cream formulation containing plantain biome-13 (5-Mix) was prepared, and the detailed composition is shown in Table 9 below.

Furtherance Content (parts by weight) Example 8-1 Example 8-2 Example 8-3 Purified water to 100 to 100 to 100 xanthan gum 0.50 0.50 0.50 Disodium EDTA 0.02 0.02 0.02 5-Mix 5.00 5.00 10.00 coco-betaine 20.00 20.00 20.00 hyaluronic acid 5.00 10.00 5.00 1,2-Hexanediol 2.00 2.00 2.00 Sorbitan Oliveate 4.00 4.00 4.00 beeswax 2.50 2.50 2.50

2) Skin clinical test to measure elasticity

The elasticity measurement was expressed as Reviscometerⓡ RVM600 and the unit of measurement was Arbiturary Unit (AU). The viscous elasticity of the skin is determined by applying a special type of shear wave to the skin surface when the probe is in contact with the skin surface. It was measured using a viscous elastic device (Reviscometerⓡ RVM600: COURAGE + KHAZAKA electronic GmbH, Germany) that quantitatively measures the skin texture condition or the viscous elasticity of the skin.

Reviscometerⓡ RVM600 measures the change in vibration frequency to calculate the stiffness/suppleness of the skin surface. The Reviscometerⓡ RVM600 was used by averaging the measured values by pressing the attached probe vertically to the skin surface and then lightly pressing it in all directions (360 degree rotation).

The moisture content of the skin has a close influence on the elasticity of the skin, and in order to maintain elastic skin, adequate moisture must be maintained in the skin. To measure the viscous elasticity of the skin, Reviscometerⓡ RVM600 was used, which measures the skin's viscosity and elasticity by measuring the resonance running time (RRT).

In order to investigate the effect of the cream containing plantain biome-13 on the viscous elasticity, the changes in the viscous elasticity at each measurement point of the three groups using each product, Examples 8-1 to 8-3, were examined for 8 weeks. analyzed.

group (N) Measurement time (mean±SD) pre After 2 weeks After 4 weeks After 6weeks After 8weeks Example 8-3 114.89±23.60 121.64±35.10 137.83±30.83 150.50±35.12 137.52±25.77 Example 8-2 109.60±29.38 110.40±25.35 123.82±31.25 139.18±37.61 134.06±33.44 Example 8-1 124.07±27.90 120.56±21.32 134.21±42.68 134.97±26.32 131.63±18.46

As a result, in the case of the group using Example 8-3 as shown in Table 10 and FIG. 8, the viscous elasticity before application of the product was 114.89±23.60AU, but after 2 weeks, 121.64±35.10AU, and after 4 weeks, 137.83±30.83 AU, 150.50±35.12AU after 6 weeks, and 137.52±25.77AU after 8 weeks, the viscous elasticity significantly increased with time. In the case of the group using Example 8-2, it was 109.60±29.38AU before application of the product, but 110.40±25.35AU after 2 weeks, 123.82±31.25AU after 4 weeks, 139.18±37.61AU after 6 weeks, 8 weeks Afterwards, it significantly increased to 134.06±33.44AU (p<0.01). In the case of the group using Example 8-1, 124.07±27.90AU before product application, 120.56±21.32AU after 2 weeks, 134.21±42.68 after 4 weeks, 134.97±26.32 after 6 weeks, 131.63 after 8 weeks The viscous elasticity increased over time to ±18.46 AU (there was no significant difference).

There was no difference in the viscous elasticity between the three groups at the time of the 5th measurement, and the change in the viscous elasticity of Example 8-3 was the largest in the difference in the amount of change in the viscous elasticity between the three groups at each measurement point over time, and Example 8- 2, Example 8-1 appeared in the order. As for the change in viscous elasticity, the group using the plantain biome-13 showed that the viscous elasticity increased over time. Taken together, the plantain biome-13 containing cream had a positive effect on the viscoelasticity compared to the cream containing no plantain biome-13. could see the madness.

Therefore, it is thought that plantain biome-13 can be usefully used as a cosmetic material for skin elasticity, and it is possible to develop various plantain biome-13 as a cosmetic material by showing that cream containing plantain biome-13 has a positive effect on the skin. has been filed.

Summarizing the above, by mixing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polyglutamic acid, excellent effects can be exerted on skin moisturizing, elasticity and skin barrier strengthening. It is thought that it can be used as a new cosmetic raw material.

Claims (6)

A cosmetic composition for moisturizing the skin containing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma glutamic acid as active ingredients.
According to claim 1,
The plantain extract,
A cosmetic composition for moisturizing the skin, characterized in that it is extracted from plantain using any one or more selected from methanol, n-hexane, methylene chloride, and ethyl acetate as an extraction solvent.
A cosmetic composition for enhancing skin elasticity containing plantain extract, fennel fruit extract, Lactobacillus plantarum culture, mugwort leaf extract and polygamma glutamic acid as active ingredients.
4. The method of claim 3,
The plantain extract,
A cosmetic composition for enhancing skin elasticity, characterized in that it is extracted from plantain using any one or more selected from methanol, n-hexane, methylene chloride, and ethyl acetate as an extraction solvent.
plantain extract, fennel fruit extract, A cosmetic composition for strengthening the skin barrier containing Lactobacillus plantarum culture, mugwort leaf extract and polygamma-glutamic acid as active ingredients.
6. The method of claim 5,
The plantain extract,
A cosmetic composition for strengthening the skin barrier, characterized in that it is extracted from plantain using any one or more selected from methanol, n-hexane, methylene chloride, and ethyl acetate as an extraction solvent.

Images