KR100532010B1 - Cosmetic composition containing persicaria thunbergii and ixeris dentata extracts stabilized with nanoliposome - Google Patents

Abstract

The present invention is stabilized with nano-liposomes, characterized in that it contains 0.01 ~ 30.0% by weight to the total weight of the cosmetic composition, the nano liposomes containing the extract extracted by mixing the moth and Gomari in a weight ratio of 1:19 ~ 19: 1 The present invention relates to a cosmetic composition containing crust and Gomari extract, which, according to the present invention, has excellent stability and improves sedimentation and discoloration even when an excess amount of Crab and Gomari extract is contained. Stabilized cosmetic compositions can be obtained, in particular cosmetic compositions in transparent or liquid form.

Description

COSMETIC COMPOSITION CONTAINING PERSICARIA THUNBERGII AND IXERIS DENTATA EXTRACTS STABILIZED WITH NANOLIPOSOME}

The present invention relates to a cosmetic composition for alleviating skin irritation, which contains the extracts of rinds and gomari extracts stabilized with nanoliposomes.

Liposomes are generally made of phospholipids. These phospholipids consist of hydrophobic long hydrocarbon chains and hydrophilic polar groups, and the hydrophobic long hydrocarbons consist of sphingosin derivatives or glycerol derivatives. The other side has ester linkages with choline, ethanol amine, serine, inositol or glycerol. Phospholipids form these regularly arranged structures in the water medium, namely micelles or lamellar structures.

Liposomes are also similar in structure to biological membranes and are being studied as membrane model systems and physiological mediators (R. Sundler. D. Papahadjpovlos, Biochim. Biophys. Acta, 648.743 (1981)). It is mainly used in emulsion-based products (patent application No. 92-2287), such as lotions and creams, in order to facilitate the delivery to the skin by collecting the ingredients to further increase the effect of these active ingredients.

Ixeris dentata NAKAI is an asteraceae plant called rapeseed. It has a bitter taste and is famous for its long-lasting herbs from late autumn to spring. According to Dongbogam, it clears the spirit of sensation and removes creepy chills due to toxins and mild fever. Usually, it is widely used as a cough medicine in oriental medicine, and it is used as a seasoning or salt pickled in spring. It is used as a medicine for fever, health, pneumonia, hepatitis, and boil. In the present invention, the above extracts using the extract extracted using a conventional extraction solvent.

Triterpenoids consist of triterpenoids such as α-Amyrin, α-Amyrin Acetate, Baurenol Acetate, β-Amine, and β-Amyrine Acetate. , Daturaolone Germanicol acetate, Lupeol acetate, Lup-20 (29) -en-3-one, Ole Olean-18-en-3-one, containing taraxasterol, and 11,13-α-dihydroglucozalu as sesquiterpenoid Zanine C (11,13-α-dehydroglucozaluzanin C), 10-α-hydroxy-8-deoxy glucoside, 8-Epideacylcynaropicrin, 8-Epideacynaropicrin glucoside, Glucozaluzanin C Ixerin, Picriside B, etc. are known to contain (M. Tamai et al., Planta Med, 1989, S. Seo et al., J. Am. Chem Soc, 1981, SC. Shin et al., CA 1993, T. Akihisa et al., Phytochemistry, 1994, W. Kisiel, Phytochemistry, 1992 H. Fuchino et al., Chem. Pharm. Bull. 1995, K. Shiojima et al , Chem. Pharm. Bull 1996, A. Hisham et al., Phytochemistry 1995).

In addition, recent studies have been actively conducted in Korea, and researches on the analysis of more diverse components of triterpenoids have been known, and its human-applied pharmacological studies have been conducted (Natural Chemical Research. Woo Won-sik, Seowol University Press) , 2001).

Persicaria thunbergii H Gross is a vine of the genus Polygonaceae. It is distributed mainly in Northeast Asia such as Korea and China. (Lee Chang-Bok: Korean Plant Book 1986, Lee Woo-Cheol: Primary Korean Plant Book 1996). In addition, the seeds are called high school vein, especially used for increasing vision and dysentery. Flavonoids known as Gomari are known as persicarin and quercitrin (Chinese medicine dictionary, Shanghai Science and Technology Publishing House, 1996). In the present invention, the above-mentioned gomari using an extract extracted using a conventional extraction solvent.

On the other hand, in general, plant extracts have problems in terms of stability when used above the effective concentration in cosmetics or pharmaceuticals. In particular, in the composition requiring transparency, problems of stability such as discoloration, precipitation, and odor are clearly revealed, and therefore, its use is limited to products such as skin lotions and essences.

This is because the plant extracts react with each other in combination with the charged compounds in the formulation, and are easily oxidized by photoreactive compounds such as ultraviolet rays to exhibit discoloration and odor.

As a solution to this problem, a method of adding a small amount of plant extract raw material to the above products has been proposed, but when containing a small amount of the plant extract has a problem that it is difficult to fully expect the efficacy of the plant extract.

An object of the present invention is to solve the problems caused when using the extracts of plants extracts goggles, gomari extract in a product that requires transparency, very high stability and high skin permeability, but also excellent efficacy and effectiveness of the extract, nano liposomes It is to provide a cosmetic composition containing the extract and gomari extract stabilized by.

In order to achieve the above object, according to the present invention, 0.01 ~ 30.0% by weight of the nano liposomes containing the extract extracted by mixing the weight and gomari in a weight ratio of 1:19 ~ 19: 1, based on the total weight of the cosmetic composition Provided is a cosmetic composition comprising the extract and gomari extract stabilized with nanoliposomes.

The present invention is described in detail as follows.

The moss and gomari can use outposts.

The moth and gomari extract are obtained as follows.

Dry moths and gomary outposts, and dry them thoroughly before mixing them. These mixing ratios are the weight ratio of 1: 19-19: 1, and it is most preferable to mix by weight ratio of 1: 1.

Subsequently, an extraction solvent is added to this mixture. As an extraction solvent, alcohol, such as methanol, isopropyl alcohol, propylene glycol, ethanol, and purified water, etc. can be used 1 type or in mixture of 2 or more types. Among them, it is preferable to use 50% 1,3-butylene glycol aqueous solution which has an antiseptic effect, hypoallergenic and can minimize the loss of vegetable polysaccharides alone or in combination with other one or more extraction solvents.

On the other hand, the extracts used in the present invention may be used separately after extracting the extracts and gomari, respectively, in the above ratio.

Then, it is heated for 4-6 hours at 50-60 ° C., and then extracted and filtered under ultra-high frequency reduced pressure of 200-300 mmHg in a 40 kHz frequency range while heating again at 80-90 ° C. for 6-8 hours to obtain an extraction crude liquid.

This was concentrated again to a concentration of about 20-30% of the solids at a high pressure of 600 ~ 900mmHg, and then treated with far infrared rays to obtain a final extract.

The far-infrared ray treatment is preferably carried out, 10 ⁴ ~ 10 ⁶ nm to 24-30 hours left at elvan vessel, known to be a far-infrared radiation of wavelength. Through this, it is possible to increase the activity of the active ingredient to increase the antiseptic effect and the stability and skin safety of the raw material.

Nanoliposomes are made of a liposome consisting of phospholipid, glutathione, polysorbate 20, and ethanol seven times through a high pressure emulsifier to reduce the size.

The bitter gourd and gomari extract is preferably added in an amount of 1.0 to 30% by weight based on the total weight of the extract-containing nanoliposomes. If it is added less than 1.0% by weight, the effect is hard to expect, and if it is added more than 30% by weight, no increase in efficacy effect is observed. As for the addition amount of an extract, 10.0 weight% is the most preferable.

When extracts are obtained using a combination of moths and gooses, their properties are dark brown, highly concentrated, similar to conventional plant extracts, and the amount of active ingredients is higher than that of conventional plant extracts. The problem is big.

By stabilizing the bitter gourd and gomari extract according to the present invention with nanoliposomes, it is possible to further increase the efficacy by adding more active ingredients obtained by complex extraction of bitter gourd and gomari to the cosmetics.

The nanoliposomes stabilized with the extract can be added to cosmetics such as nourishing lotion, nourishing cream and essence, and in particular can be added to liquid or transparent cosmetics.

Nanoliposomes stabilized by the moth and gomari extract are preferably contained in an amount of 0.01 to 30.0% by weight based on the total weight of the cosmetic composition. If the content is less than 0.01% by weight, it is difficult to expect an effective effect, and if the content is more than 30.0% by weight, no increase in efficacy is observed. As for content, 10-30 weight% is the most preferable.

The following Examples, Comparative Examples, Preparation Examples, and Test Examples are provided to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

(Example)

(Example 1)

Preparation of Crust and Gomari Extracts

Each 50 g of dried moth and gourd outposts were mechanically ground until there was no change in weight. Crushed 600 g of 50% 1,3-butylene glycol was added to 100 g, and then heated at 50 ° C. for 4 hours, followed by extraction at 250 ° C. in a high frequency reduced pressure of 250 mmHg while heating at a high temperature of 85 ° C. for 6 hours. Subsequently, it was filtered through a 400 mesh sieve and again filtered using filter paper no. 4 (Whatman filter paper, Fast 4 qualitative). This was concentrated again to a concentration of about 20% of a dark brown solid at a pressure of 800mmHg in a vacuum concentrated flask, and left for 24 to 30 hours in an ganban rock vessel to prepare the extracts.

(Comparative Example 1 and Comparative Example 2)

Except for using 100 g each of the moth and gourd outpost, the moth extract and gourd extract were prepared in the same manner as in Example 1 to prepare Comparative Examples 1 and 2.

(Test Example 1)

Histamine anti-inhibitory test

The histamine anti-inhibitory test was carried out using Example 1, Comparative Examples 1 and 2, which contained Gomari and Mugwort extracts. As a control, the extract was not added, and the experimental animals were divided into groups.

As the experimental animals, rat sprague-dawley (male, 10 weeks old) 300-350 (g / rat), which is mainly used as a biomedical research model including toxicology and pharmacology, was purchased from Samtaco Bio Korea. As a control, a histamine (Sigma) free trigger compound Compound (compound) 48/80 (sigma C2313) was used.

First, the back of the rat was carefully epilated with a hair remover, and the first 4 histamine injection sites were selected first. Samples of each group as shown in the following table in two of the four positions 0.5g, the other two positions were applied as a control. After 1 hour, the second application was carried out at the same position as the first, and after 1 hour, 0.05 ml of 0.5% histamine was intradermally injected with the base material. After intradermal injection, 1.5% Evans Blue (sigma E2129) was injected intravenously (1.5 ml) to confirm blue infection. After 45 minutes, the rats were cervically distal and the skin was carefully dissected off. The long and short diameters of the blue spots were measured, respectively, and the area was determined.

Using the obtained blue salt area, the histamine anti-inhibition rate was calculated according to the following formula.

The results are shown in Table 1 below.

sample Extract ratio (Crowbar: Gomari) Histamine hyperactivity inhibition rate (%) Example 1 0.10: 19.9 6.3 1.00: 19.0 42.5 5.00: 15.0 51.5 10.0: 10.0 89.7 15.0: 5.00 53.4 19.0: 1.00 39.6 19.9: 0.10 26.9 Comparative Example 1 20.0: 0.00 15.8 Comparative Example 2 0.00: 20.0 15.9

In Table 1, it was found that the extract and gomari extract were excellent in histamine anti-inhibition rate at a mixing ratio of 1.0: 19.0 to 19.0: 1.0.

(Example 2)

Preparation of Nanoliposomes with Stabilized Mugwort and Gomari Extracts

Phospholipids, glutathione, 95% modified ethanol, glycerin, polysorbate 20 were emulsified under high pressure to form closed bilayered liposomes. That is, the phase A of the following Table 2 was heated up to 95 degreeC, and it mixed and melted uniformly. After cooling to 40 ° C., phase B containing the extract of gooseberry and gomari extract prepared in Example 1 was added and mixed. The mixed solution was passed through a high pressure emulsifier (Microfludizer M210EH, Microfluidies, USA) seven times under a pressure of 1200 bar and a flow rate of 500 m / s to obtain a nanoliposome that stabilized the moth and golia extract.

The size of the liposome obtained through one pass was about 133 nm, but the size of the seventh was about 75 nm.

ingredient weight% A Phospholipids Phosphatidylcholine 3.1 Phosphatidylethanolamine 0.55 Phosphatidylserine 0.5 Glutathione 0.15 95% ethanol 20 glycerin 30 Polysorbate 20 4 B Extract of Example 1 30 Purified water Remaining amount system 100

(Comparative Example 3)

Preparation of Nanoliposomes

Nanoliposomes were prepared in the same manner as in Example 2, except that the extracts of Goji and Gomari extract of Example 1 were not added.

(Production Examples 1 to 6)

In Table 3a, phase D and phase E were completely dissolved, respectively, and then phase D was added to phase E and stirred at 3000 rpm or more. Subsequently, the phase C was added thereto and stirred at 3000 rpm or more to prepare a lotion containing nanoliposomes in which the moth and gomari extracts were stabilized.

(Comparative Production Examples 1 to 7)

A cosmetic was prepared in the same manner as in Preparation Example 1, except for using the C-phase shown in Tables 3a and 3b.

(Test Example 2)

Human patch experiment

In order to evaluate the stimulating effect, after the pretreatment with Preparation Examples 1 to 6 and Comparative Preparation Examples 1 to 7 were compared with the stimulation inhibitory effect. The experimental methods and procedures were followed the procedure of the effect of protective cream on irritant (Walter Wigger-Alberti et al. J Am Acad Dermatol. 1999).

Twenty healthy adult men and women (10 males, 10 females) were coated with 0.03 ml of Preparation Examples 1 to 6 and Comparative Preparation Examples 1 to 7 on the inner skin of the upper arm.

After 30 minutes of application, 10% sodium lauryl sulfate as an irritant was attached to the pin chamber (diameter 12 mm. Epitest Ltd Oy. Finland) for 30 minutes and then removed. One hour after removal, the degree of stimulation was assessed by visual observation, measurement of epidermal moisture loss, and erythema index.

All the above experiments were carried out at the same place with a temperature of 22 ± 2 ℃, relative humidity 55-60%.

(1) Visual observation

Evaluation method was evaluated by dividing into six grades as follows, the average value of 20 people are shown in Tables 4a and 4b.

0: no reaction

1: very slight erythema

2: slight erythema

3: erythema, border edema, papule

4: erythema, edema

5: severe erythema, edema, blisters

According to the Table 4a, 4b, bitter gourd and Gomari extract (Comparative Preparation Example 5) were superior to the synergistic effect compared to the case where the bitter hump extract and Gomari extract were used (Comparative Preparation Examples 6 and 7, respectively), according to the present invention Cosmetic preparations containing liposome stabilized bitter gourd and gomari extracts (preparation example 4) had a much better skin irritation effect compared to the use of bitter gourd and gori extract that was not stabilized with nanoliposomes (comparative example 4). In addition, the nano-liposomes containing bitter gourd and Gomari extract extracts were excellent in stimulating the stimulation when contained in 0.01 to 30% by weight based on the total weight of the cosmetic composition.

(2) Measurement of Transepidermal Water Loss (TEWL)

When the skin is irritated, the stratum corneum is damaged and the epidermal moisture loss is increased. Therefore, the epidermal moisture loss measuring device (Tewameter TM-210, Courage + Khazaka Koln, Germany) is used to measure the hard epidermal moisture loss. Evaluated.

The results are shown in Tables 5a and 5b below (unit: mg / m 2 / hr).

According to Tables 5a and 5b, bitter gourd and gomari extract (Comparative Preparation Example 5) were superior to synergistic effect when the bitter bark extract and Gomari extract were used (Comparative Preparation Examples 6 and 7), respectively, according to the present invention. Cosmetic preparations containing Liposome stabilized bitter gourd and Gomari extract extract (Manufacturing Example 4) reduced the skin irritation effect by reducing the epidermal moisture loss compared to the case of using non-stabilized bittersweet and Gomari extract extract (Comparative Example 4). Was much better. In addition, the nano-liposomes containing bitter gourd and Gomari extract extracts were excellent in stimulating the stimulation when contained in 0.01 to 30% by weight based on the total weight of the cosmetic composition.

(3) Measurement of erythema score

When the skin is irritated, erythema, blisters, and redness of the skin become red. The degree of redness can be measured using a colorimeter (Chromameter CR-300, Minolta, Japan).

The erythema index is expressed in three dimensions, L * a * b *. Among them, a * value, which is a value between red and green (negative value), was measured. . The results are shown in Tables 6a and 6b below.

According to Tables 6a and 6b, the cosmetics containing the extracts prepared by the nanoliposomes stabilized with bitter gourd and gomari extract (Preparation Example 4) were used when the extracts which were not stabilized with nanoliposomes were prepared by the extract (comparative preparation example 4). Compared with), the erythema index was lower and the skin irritation effect was much better. In addition, the nano-liposomes containing bitter gourd and Gomari extract extracts were excellent in stimulating the stimulation when contained in 0.01 to 30% by weight based on the total weight of the cosmetic composition.

(Test Example 3)

Stability experiment

Formulation stability for the cosmetics containing the extract and Gomari extract stabilized with nanoliposomes according to the present invention was measured by the following method.

The lotions of Preparation Examples 1 to 6 and Comparative Preparation Examples 1 to 7 were stored in an opaque cosmetic glass container in a thermostat maintained at 45 ° C. for 12 weeks, and kept in a completely shaded refrigerator maintained at 4 ° C. The separation, discoloration, and precipitation of the samples stored in an opaque vitreous container for 12 weeks and stored for 12 weeks in a circulation chamber (once / day) circulating at -5 ° C to 37 ° C were measured. .

Product separation and discoloration degree was evaluated by classifying the following six grades are shown in Tables 7a and 7b.

Product separation and discoloration evaluation criteria:

0: No change 1: Very little separation (discoloration)

2: Slightly separated (discolored) 3: Slightly separated (discolored)

4: Extremely separated (discolored) 5: Extremely separated (discolored)

According to Tables 7a and 7b, Preparation Examples 1 to 6 containing the extracts and the extracts stabilized with nanoliposomes of the content according to the present invention were stable without discoloration or separation symptoms at 4 ° C, 45 ° C, and circulation tests. Comparative Preparation Examples 1 to 3 outside the above content were discolored in the circulation test. Also, when the extract was not stabilized with nanoliposomes, some separation and discoloration occurred at 4 ° C, 45 ° C, and circulation tests.

The degree of product precipitation is also shown in Tables 8a and 8b below.

Product sedimentation evaluation criteria:

0: no change 1: very slight precipitation

2: little precipitate 3: little precipitation

4: settling heavily 5: settling extremely severe

In Tables 8a and 8b, Preparation Examples 1 to 6 containing the extracts and bitter gourds stabilized with the nanoliposomes of the present invention did not generate any precipitation, but Comparative Preparation Examples 1 to 3, which deviated from the contents, were subjected to the circulation test. Precipitation occurred at. In addition, precipitation was observed at 45 ° C., 4 ° C. and circulation when the extract was not stabilized with nanoliposomes.

As such, addition of the moth and gourd extracts without stabilization readily reacts with other weak ionic polymers in the formulation, causing precipitation and discoloration. However, in the case of applying the formulation to stabilize the bitter gourd and Gomari extract with nanoliposomes as in the present invention, it can be seen that precipitation and discoloration reaction are minimized even if the bitter gourd and Gomari extract is added in excess.

(Manufacture example 2)

Lotion (skin lotion)

(Manufacture example 3)

Concentrated Cosmetic Water (Essence: Solubilized Type)

(Manufacture example 4)

pack

According to the present invention, the stability and discoloration are improved even when the extracts of bitter gourd and gomari are contained in an excellent stability. A composition can be obtained.

Claims (2)

Nanoliposomes stabilized with nanoliposomes, characterized in that it contains 0.01 ~ 30.0% by weight relative to the total weight of the cosmetic composition, the nano liposomes containing the extract extracted by mixing the weights and gomary in a weight ratio of 1:19 ~ 19: 1 Gomari extract containing cosmetic composition. The method of claim 1, The bitter gourd and gomari extract, 1.0 ~ 30% by weight relative to the bitter gourd and Gomari extract containing nano liposomes, Stabilized bitter gourd and Gomari extract containing cosmetic composition.