KR102307139B1 - Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Chondrus crispus, Origanum majorana, Petroselinum crispum and Fumaria officinalis As Active Ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing, as an active ingredient, a mixed extract of Irish moss (Chondrus crispus), marjoram (Origanum majorana), carum petroselinum (Petroselinum crispum), and round comb sagebrush (Fumaria officinalis) as an active ingredient, in more detail relates to a skin moisturizing cosmetic composition having excellent skin moisturizing effect and skin barrier improvement effect.

Description

Cosmetic Composition For Moisturizing Skin Comprising Mixed Extracts of Chondrus crispus, Origanum majorana, Petroselinum crispum and Fumaria officinalis As Active Ingredient}

The present invention relates to a cosmetic composition comprising, as an active ingredient, a mixed extract of Irish moss, marjoram, karum petrosellinum, and rhododendron, and more particularly, to a cosmetic composition for moisturizing the skin.

The goal of cosmetics is to maintain beautiful and healthy skin, and to delay the aging of the skin that occurs naturally with age. In general, aging is caused by oxidative damage to biological materials by active oxygen, such as hydrogen peroxide, hydroxy radicals, and superoxide radicals. That is, reactive oxygen species peroxidize the lipids constituting the cell membrane, destroy the cell membrane, oxidize proteins, and attack hexane to cause oxidative damage, destroying biological materials and causing cell death. If this phenomenon continues, aging of the skin progresses, and wrinkles, loss of elasticity, and a crumbly appearance appear on the skin.

As we age, our skin becomes rough and dark, as well as wrinkles and loss of elasticity, as well as a decrease in metabolism and blood circulation disorders. The reason the skin becomes rough is because of the lack of moisture. In general, the moisture content of the skin consists of about 70% in the dermis layer and about 30% to 10% in the epidermis layer. In the epidermal layer with keratinocytes, there are ingredients that have a hydrophilic structure called Natural Moisturizing Factor (NMF) to hold water molecules and retain moisture. Natural moisturizing factors include sebum components, amino acids, lactic acid, urea, and inorganic salts. These factors keep the moisture of the epidermal layer at about 30-10%, making the skin smooth and soft.

For this reason, it is very important to prevent skin aging to properly maintain the moisture content of the epidermal layer of the skin. However, natural moisturizing factors such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, and urea, which are commonly used in cosmetics, can improve moisturizing power, but have strong electrolytic properties and thus emulsification stability of cosmetics. There is a problem that harms Amino acids, collagen, and elastin also have moisturizing properties, but there is a limit to their moisturizing ability. In addition, substances such as ascorbic acid, DL-α-tocopherol, and Dibutyl Hydroxy Toluene (BHT) have excellent antioxidant effects and prevent skin aging. Maintains normal hydration. However, since these substances are used as synthetic products, they may cause skin stability problems when used for a long period of time or in large amounts. Therefore, the development of a substance that is safe, usable, and has a high moisturizing effect like a natural moisturizing factor in external skin preparations is one of the important research tasks in the cosmetic industry.

Therefore, research on skin aging and moisture improvement is expected to continue, and many cosmetics using natural products are being developed to solve these skin problems and reduce skin irritation caused by various external factors. Natural ingredients have fewer side effects on the skin, and as consumer response increases, the value of development as a cosmetic raw material is increasing. For this purpose, research to develop substances with excellent safety and excellent skin-beauty effects by searching the skin physiological activities of various natural materials is currently being actively conducted.

Various patent documents have been published on the moisturizing effect of plant extracts. Korean Patent No. 10-1814498 discloses a cosmetic composition containing a mixed extract of eucalyptus leaves and red shamrock leaves to have skin protection effects such as skin moisturizing enhancement and skin irritation relief. In addition, Korean Patent No. 10-1926263 discloses a cosmetic composition containing marjoram, Molochia, and lemon balm mixed fermented extract to improve skin wrinkles, skin whitening, skin moisturizing, skin irritation relief and antioxidant effects.

Accordingly, the present inventors studied the possibility of application to cosmetics in various natural materials that were not known so far, and as a result, Irish Moss, Marjoram, Carum Petroselinum, and Round Comb Cordyceps were selected and extracts were prepared from them to measure the moisturizing effect. and found it to be very effective.

It is an object of the present invention to provide a cosmetic composition for skin protection that contains a mixed extract of Irish Moss, Marjoram, Carum Petroselinum, and Round Comb Cordyceps and shows a moisturizing effect.

In addition, an object of the present invention is to prepare a supercritical extract and an ultrasonic extract together with a solvent extract in order to maximize the efficacy of the mixed extract of Irish moss, marjoram, carum petrosellinum, and rhododendron, and show excellent effects through a purification process. To provide a cosmetic composition.

The present invention relates to a cosmetic composition for moisturizing the skin containing a mixed extract of Irish moss, marjoram, karum petrosellinum and rhododendron.

Preferably, the cosmetic composition has an effect of improving the skin barrier function.

Also preferably, the mixed extract of Irish moss, marjoram, carum petrosellinum, and rhododendron may be contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

Also, preferably, the Irish moss, marjoram, carum petrosellinum and the mixed extract of the round comb sagebrush (a) purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether , butylene glycol, hexane, and a solvent extraction method using one or more solvents selected from the group consisting of a mixture thereof, (b) supercritical extraction method or (c) extraction method selected from the group consisting of ultrasonic extraction method characterized in that the extraction do it with

Also preferably, the mixed extract is characterized in that it contains Irish moss, marjoram, carum petrosellinum, and round comb coyote in a mixed weight ratio of 1 to 3:1 to 3:1 to 3:1 to 3.

Also preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing agent, oil, powder foundation, emulsion foundation, wax foundation and spray. It may have any one formulation selected from

The mixed extract of Irish Moss, Marjoram, Carum Petroselinum and Round-comb Cordyceps according to the present invention is excellent in moisturizing effect and skin barrier function recovery effect.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and have the meanings as commonly understood by one of ordinary skill in the art of the present invention. In addition, although preferred methods and samples are described herein, similar or equivalent ones are also included in the scope of the present invention.

The term "about" refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless the context requires otherwise, the terms "comprises" and "comprising" include the steps or elements presented, or groups of steps or elements, but any other step or element, or It is to be understood that a step or group of elements is not excluded.

The present invention relates to a cosmetic composition for moisturizing the skin containing a mixed extract of Irish moss, marjoram, karum petrosellinum, and rhododendron.

Irish moss ( Chondrus crispus ) is a red alga belonging to the genus Chondrus, and using hot water and hot alkaline aqueous solution from Irish moss, ι (Iota)-carrageenan, κ (Kappa)-carrageenan, λ (Lambda)-carrageenan, etc. ingredients can be extracted.

Marjoram ( Origanum majorana ) is a perennial plant of the Lamiaceae family, which grows on the Mediterranean coast, has a strong scent, and has been used medicinally and edible since ancient times, and is often used as a tea.

Carum Petroselinum crispum ) is a component obtained by extracting the above-ground part of parsley or parsley outpost.

Round Comb Coral ( Fumaria) officinalis ) is an annual plant of the dicotyledonous plant, Papaveraceae, and its name means that it has round, spherical fruits and the leaves are deeply and thinly split like a comb, resembling a coral color.

In the present invention, the mixed extract of Irish moss, marjoram, carum petrosellinum, and rhododendrons means obtained by extraction from various organs or parts of irishmos, marjoram, carum petrosellinum and rhododendron. Preferably, it can be extracted from the leaves of Irish moss, Carum Petroselinum, and rhododendron and marjoram.

In addition, the mixed extract of Irish moss, marjoram, carum petrosellinum and rhododendron may be prepared according to a conventional method known in the art. For example, extraction is performed using (a) solvent extraction, (b) supercritical extraction using reduced pressure and high temperature with carbon dioxide, or (c) ultrasonic extraction.

In the present invention, the mixed extract of Irish moss, marjoram, carum petrosellinum, and round comb coyote can be prepared using a variety of extraction solvents, for example, water, anhydrous or hydrous lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol). And butanol), propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, at least one extraction solvent selected from the group consisting of nucleic acids and mixtures thereof It can be obtained using, preferably obtained using ethanol, 70% (v/v) ethanol or water, and most preferably obtained using 70% (v/v) ethanol.

On the other hand, those skilled in the art that the mixed extract of Irish moss, marjoram, carum petrosellinum, and rhododendron of the present invention can obtain extracts exhibiting substantially the same effect using other extraction solvents as well as the above-described extraction solvents. is self-evident to

In addition, the mixed extract of Irish moss, marjoram, carum petrosellinum, and rhododendron of the present invention includes not only the extract using the above-mentioned extraction solvent, but also an extract that has undergone a conventional purification and fermentation process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by extraction using ultrasound, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity The active fraction obtained through various purification and extraction methods additionally performed, such as separation by a method prepared for separation according to sex) or an extract using a fermentation product using a natural state or various microorganisms, is also included in the extract of the present invention.

In addition, the present invention provides a cosmetic composition, characterized in that the extract is obtained by concentrating the extract under reduced pressure or freeze-drying a liquid obtained by cooling and heating at room temperature, and further, a solvent.

The extraction method by the supercritical extraction method by the reduced pressure by carbon dioxide and the high temperature refers to the supercritical fluid extraction method, and in general, the supercritical fluid is a liquid having a gas when the gas reaches a critical point under high temperature and high pressure conditions, and It has gaseous properties and chemically has a polarity similar to that of a non-polar solvent. Due to these properties, supercritical fluids are used for the extraction of fat-soluble substances (J. Chromatogr. A. 1998;479:200-205).

Carbon dioxide becomes a supercritical fluid with both liquid and gas properties as the pressure and temperature reach the critical point due to the operation of the supercritical fluid device, and as a result, the solubility in fat-soluble solutes increases. When the supercritical carbon dioxide passes through an extraction vessel containing a certain amount of the sample, the fat-soluble substance contained in the sample is extracted into the supercritical carbon dioxide.

After extracting the fat-soluble substance, if supercritical carbon dioxide containing a small amount of cosolvent is flowed through the sample remaining in the extraction vessel again, components that were not extracted with pure supercritical carbon dioxide alone can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract the active ingredient by using supercritical carbon dioxide or a mixed fluid in which a cosolvent is additionally mixed with carbon dioxide.

As the cosolvent, one or a mixture of two or more selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acid and diethyl ether may be used. Preferably, one or a mixture of two or more selected from the group consisting of olive oil, ethanol, methanol, and water may be used.

Most of the extracted samples contain carbon dioxide, and since carbon dioxide is volatilized into the air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the cosolvent can be removed with a vacuum evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration, and ultrasonic waves can destroy an insoluble solvent contained in a sample in an aqueous solvent, and due to the high local temperature generated at this time, Because the kinetic energy of the reactant particles is increased, sufficient energy required for the reaction is obtained, and the high pressure is induced by the impact effect of ultrasonic energy to increase the mixing effect of the material and the solvent contained in the sample, thereby increasing the extraction efficiency.

The extraction solvent that can be used for the ultrasonic extraction method may be one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid, and diethyl ether. The extracted sample is vacuum filtered to recover the filtrate, then removed by a vacuum evaporator, and an extract can be obtained through a conventional extract preparation method of freeze-drying.

According to the present invention, the mixed extract of Irish moss, marjoram, carum petrosellinum, and round comb coyote is contained in an amount of 0.001 to 30.0% by weight, preferably 0.01 to 20% by weight, based on the total weight of the cosmetic composition, and more Preferably 0.1 to 10% by weight is contained. When the content of the extract is less than 0.001% by weight, the skin moisturizing effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin moisturizing effect for the content increase is insignificant, there is a problem in safety and stability of the formulation, and economical not even an enemy

The mixed extract according to the present invention may include Irish moss, marjoram, carum petrosellinum, and rounded comb coyote in a mixed weight ratio of 1 to 3:1 to 3:1 to 3:1 to 3, preferably 1 It may be included in a mixing weight ratio of 1:1:1:1.

After removing unnecessary ingredients through a purification process in order to use the mixed extract of Irish moss, marjoram, carum petrosellinum, and rhizome prepared by a conventional method in a cosmetic formulation, it may include a cosmetic composition that exhibits efficacy. The purification process may be any conventional purification process, and liquid-liquid extraction using a difference in solubility, precipitation and a filter, and chromatography using the size of polarity and molecular weight, etc. may be used. Also, for example, after dissolving the mixed extract of Irish Moss, Marjoram, Carum Petroselinum, and Round Comb Cordyceps in ethanol aqueous solution (50% (v/v), 70% (v/v) solution, etc.) or other aqueous solution, HP A concentrate can also be prepared by separating the water-soluble material and the oil-soluble material using a column packed with -20, sephadex column by using the difference in polarity and molecular weight, then concentrating the oil-soluble material by TLC and freeze-drying.

The ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the active ingredient as an active ingredient, for example, conventional antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances. adjuvants, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream, spray, etc., but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals Adult cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

In the case of cleansing in which the formulation of the present invention contains a surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid as carrier components Amide ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream , cleansing lotion, cleansing water, and cleansing gel, but not limited thereto.

Hereinafter, the present invention will be described in more detail through a preparation example. These preparation examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these preparation examples according to the gist of the present invention. .

Preparation Example 1 : Irish Moss, Marjoram, Carum Petroselinum, Round Comb Cordyceps Mixed Extract

After washing Irish moss outpost, marjoram leaf, carum petrosellinum outpost, and round comb-colored outpost, rapidly cooled at -70°C, freeze-dried at -50°C and 5 mtorr for 3 days, and then thoroughly using a grinder. It was pulverized to prepare a powder. After mixing 60 g each of the prepared Irish moss outpost, marjoram leaf, carum petrosellinum outpost, and round-bite-colored outpost, it was extracted with 70% (v/v) ethanol solution at reflux 3 times for 5 hours each, and cooled. Then, it was filtered with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried Irish moss, marjoram, carum petrosellinum, and the mixed extract powder of the round comb coyote was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 2 : Irish Moss, Marjoram, Carum Petroselinum, Supercritical Fluid Mixed Extract of Round Comb Cordyceps

After mixing 60 g each of the prepared Irish moss outpost, marjoram leaf, carum petrosellinum outpost, and round-toothed outpost, this is followed by a conventional supercritical extraction method (at a temperature of about 60°C and a pressure of about 300 atmospheres (atm)). Extracted using olive oil as a co-solvent under supercritical state under To obtain a mixed extract powder of a round-comb coral color. This was used by dissolving it in 50% 1,3-butylene glycol.

Preparation Example 3 : Preparation of ultrasonically mixed extracts of Irish moss, marjoram, carum petrosellinum, and round comb

After mixing 60 g each of the prepared Irish moss outpost, marjoram leaf, carum petrosellinum outpost, and round-toothed outpost, it is ultrasonicated (using a supernonic ultrasonic device at 25 KHz at 30°C for about 2 hours). extraction), and after removing salts using an ultrafiltration membrane, 7.1 g was obtained through concentration under reduced pressure and freeze-drying. The dried and prepared mixed extract powder of Irish moss, marjoram, carum petrosellinum, and round-comb coyote was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 4 : Irish Moss, Marjoram, Carum Petroselinum, Round Comb Cordyceps Mixed Extract

After mixing 96g, 48g, 48g, and 48g of the prepared Irish Moss outpost, Marjoram leaf, Carum Petroselinum outpost, and round comb-colored outpost, respectively, it was mixed with 70% (v/v) aqueous ethanol solution for 3 hours each for 5 hours. After extraction under reflux, cooling, and filtering with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried Irish moss, marjoram, carum petrosellinum, and the mixed extract powder of the round comb coyote was dissolved in 50% 1,3-butylene glycol and used.

Preparation Example 5 : Irish Moss, Marjoram, Karum Petroselinum, Round Comb Cordyceps Mixed Extract

After mixing 48g, 96g, 48g, and 48g of the prepared Irish Moss outpost, Marjoram leaf, Carum Petroselinum outpost, and round-toothed outpost, respectively, it was mixed with 70% (v/v) aqueous ethanol solution for 3 hours for 5 hours each. After extraction under reflux, cooling, and filtering with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried Irish moss, marjoram, carum petrosellinum, and the mixed extract powder of the round comb sagebrush were used by dissolving in 50% 1,3-butylene glycol.

Preparation Example 6 : Irish Moss, Marjoram, Carum Petroselinum, Round Comb Cordyceps Mixed Extract

After mixing 48g, 48g, 96g, and 48g of the prepared Irish Moss outpost, Marjoram leaf, Carum Petroselinum outpost, and round-toothed outpost, respectively, it was mixed with 70% (v/v) aqueous ethanol solution for 3 hours each for 5 hours. After extraction under reflux, cooling, and filtering with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried Irish moss, marjoram, carum petrosellinum, and the mixed extract powder of the round comb coyote was dissolved in 50% 1,3-butylene glycol and used.

manufacturing example 7: Irish Moss , marjoram , carum Petroselinum , round comb cordial color mixed extract

After mixing 48g, 48g, 48g, and 96g of the prepared Irish Moss outpost, Marjoram leaf, Carum Petroselinum outpost, and round-toothed outpost, respectively, it was mixed with 70% (v/v) aqueous ethanol solution for 3 hours each for 5 hours. After extraction under reflux, cooling, and filtering with Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. or less and freeze-dried to obtain 6.7 g. Then, the dried Irish moss, marjoram, carum petrosellinum, and the mixed extract powder of the round comb coyote was dissolved in 50% 1,3-butylene glycol and used.

Formulation example

Cosmetics (Formulation Examples 1 to 7) each containing the mixed extract of Irish Moss, Marjoram, Carum Petroselinum, and Round Comb Cordyceps of Preparation Examples 1 to 7 were prepared as shown in the composition of Table 1 below. In addition, for comparison of efficacy, a cosmetic composition containing glycerin and 1,3-butylene glycol as a moisturizer (comparative formulation example 1), extract or A cosmetic composition (Comparative Formulation Example 2) not containing all of the moisturizer was prepared as shown in Table 1 below.

Ingredients (wt%) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Formulation Example 7 Comparative Formulation Example 1 Comparative Formulation Example 2 Preparation Example 1 5.0 - - - - - - - - Preparation 2 - 5.0 - - - - - - - Preparation 3 - - 5.0 - - - - - - Preparation 4 - - - 5.0 - - - - - Preparation 5 - - - - 5.0 - - - - Preparation 6 - - - - - 5.0 - - - Preparation 7 - - - - - - 5.0 - - glycerin - - - - - - - 5.0 - 1.3 Butylene Glycol - - - - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 microcrystallin 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 squalane 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 liquid paraffin 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 trioctanoin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl Acetate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 cyclomethicone 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 0.05 fragrance, preservative appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount appropriate amount Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

Experimental Example 1 : Skin electrical conductivity

The clinical moisturizing effect and lasting power of the cosmetic compositions of Formulation Examples 1 to 7 were measured as follows. Through the survey, 125 healthy adult men and women who felt dry skin were randomly formed into groups of 25, and Formulation Examples 1 to 7 and Comparative Formulation Examples 1 and 2 suitable for each group were administered twice a day for 4 weeks, respectively. was applied to both faces one by one. The moisturizing effect was evaluated by quantifying the increase rate (%) of the skin electrical conductivity according to the amount of epidermal moisture using Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the actual use test and the improvement effect after the end. The experimental results are shown in Table 2 below.

division before use 2 weeks after use 4 weeks after use Formulation Example 1 26.76 42.61 52.52 Formulation Example 2 26.95 41.08 51.05 Formulation Example 3 26.20 45.23 56.35 Formulation Example 4 23.28 38.88 46.48 Formulation Example 5 24.96 36.54 44.40 Formulation Example 6 26.60 35.69 43.15 Formulation Example 7 25.52 28.87 42.59 Comparative Formulation Example 1 24.73 33.33 40.00 Comparative Formulation Example 2 27.15 25.20 28.88

As shown in Table 2 above, as a comparative formulation, even in comparison with a cosmetic containing a chemical moisturizer (Comparative Formulation Examples 1 and 2), it contains the Irish Moss, Marjoram, Carum Petroselinum, and Round Comb Cordyceps mixed extract of the present invention. It was confirmed that the moisturizing effect of the cosmetics (Formulation Examples 1 to 7) was more excellent. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 7, it was confirmed that mixing at a mixing ratio of 1:1:1:1 was most preferable since the efficacy of Formulation Example 1 was excellent.

Experimental example 2: skin barrier improvement effect

This study was conducted on 25 healthy women indoors at 25℃, 45% relative humidity, and no air flow. Skin barrier damage was induced by using a 1% aqueous solution of sodium lauryl sulfate (SLS), and after applying the cosmetic compositions of Formulation Examples 1 to 7 and Comparative Formulation Examples 1 and 2 to the upper arm of the arm, TEWAMETER TM210 (C+K eletronic GmhH. Germany) was used to measure the amount of transdermal water loss. That is, the skin barrier recovery rate (%) was confirmed by measuring the TEWL (Transepidermal Water Loss) value according to the change in TEWL value over time. The experimental results are shown in Table 3 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 50.78 65.98 83.25 98.45 Formulation Example 2 52.06 64.96 86.36 99.20 Formulation Example 3 54.82 67.45 84.99 98.86 Formulation Example 4 46.53 54.24 68.85 78.56 Formulation Example 5 41.22 54.86 71.24 80.14 Formulation Example 6 45.93 56.15 68.32 70.98 Formulation Example 7 34.55 52.24 56.83 68.42 Comparative Formulation Example 1 40.07 52.23 59.64 66.78 Comparative Formulation Example 2 39.65 49.62 53.74 58.22

As shown in Table 3 above, Formulation Examples 1 to 7 containing the Irish Moss, Marjoram, Carum Petroselinum, and Round Comb Cordyceps mixed extract did not contain Irish Moss, Marjoram, Karum Petroselinum, and Round Comb Cordyceps mixed extract. It can be seen that the amount of transdermal water loss is improved compared to Comparative Formulation Examples 1 and 2, which are not. In addition, when comparing Formulation Example 1 and Formulation Examples 4 to 7, it was confirmed that mixing at a mixing ratio of 1:1:1:1 was most preferable since the efficacy of Formulation Example 1 was excellent.

Experimental example 3: Safety test

A human skin patch test was performed to determine whether the cosmetic compositions of Formulation Examples 1 to 7 were irritating to the skin. The test was conducted on 20 women in their 20s and 30s who have never had a hypersensitivity reaction to skin irritation in their past history and who do not currently have skin diseases or skin allergies. Comparative Formulation Examples 1 and 2 were used as control examples.

First, the test site was wiped with 70% ethanol and dried. The prepared test material was dropped into the Finn chamber (100X10, EPITEST) and then sealed and applied to the back of the test subject. The patch was applied for 24 hours, and 1 hour and 24 hours after the patch was removed, the presence or absence of erythema and edema at the test site was observed using a magnifying glass (8MC-150, DAZOR).

The skin reaction was determined according to the regulations (Table 4) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated according to Equation 1 below, and the results are shown in Table 5.

sign score Evaluation standard - 0 no response ± 0.5 faint or mild erythema + One Demarcated but mild erythema, edema and papules ++ 2 Distinct erythema, papules and vesicles +++ 3 Severe erythema and blistering, crusting

division 24 hours 48 hours Average skin reaction
(n=20) ± + ++ ± + ++ Formulation Example 1 One - - - - - 0.14 Formulation Example 2 - - - - - - 0.28 Formulation Example 3 One - - - - - 0.28 Formulation Example 4 One - - One - - 0.14 Formulation Example 5 2 - - - - - 0.14 Formulation Example 6 2 - - - - - 0.28 Formulation Example 7 One - - One - - 0.14 Comparative Formulation Example 1 2 - - - - - 0.28 Comparative Formulation Example 2 2 - - - - - 0.00

As shown in Table 5 above, since the cosmetic compositions of Formulation Examples 1 to 7 did not cause skin irritation, the safety of the cosmetic formulations containing Irish Moss, Marjoram, Carum Petroselinum, and Round Comb Cordyceps extract was not abnormal. Could know.

As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

Irish moss (Chondrus crispus), marjoram (Origanum majorana), carum petroselinum (Petroselinum crispum) and round comb Corydalis (Fumaria officinalis) in a weight ratio of 1 to 3:1 to 3:1 to 3:1 to 3 A cosmetic composition for skin moisturizing, which contains a mixed extract as an active ingredient and has an effect of improving skin barrier function. The cosmetic composition for skin moisturizing according to claim 1, wherein the mixed extract is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. The method according to claim 1, wherein the mixed extract of Irish moss, marjoram, carum petrosellinum, and round comb coyote is (a) water, anhydrous or hydrated lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone , ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, a solvent extraction method using an extraction solvent selected from the group consisting of nucleic acids and mixtures thereof, (b) supercritical extraction method or (c) ultrasonic extraction method. The cosmetic composition for moisturizing the skin. delete delete