KR20210025259A - Cosmetic composition for moisturizing skin comprising extract of tuber magnatum as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition containing an extract of Tuber magnatum as an active component. More specifically, the present invention relates to a cosmetic composition for skin protection having an excellent skin moisturizing effect.

Description

A moisturizing cosmetic composition containing white western pine tree extract as an active ingredient {COSMETIC COMPOSITION FOR MOISTURIZING SKIN COMPRISING EXTRACT OF TUBER MAGNATUM AS ACTIVE INGREDIENT}

The invention as a white truffle (Tuber magnatum) extract on the cosmetic composition containing, as an active ingredient, and more particularly, to a skin moisturizing effect on the skin, excellent protection of the cosmetic composition.

The human body contains about 70-75% of moisture, and the skin protects the body from various external stimuli and at the same time has a barrier function that blocks moisture from the inside. And the amount of moisture contained in the skin is about 15-20%, and it is present in the skin along with oil. In the epidermal layer of the skin, it develops into a thin stratum corneum while having moisture through the process of being differentiated. Structurally, the cells of the stratum corneum act like bricks, and the intercellular lipids of the keratinocytes act as mortar to form the skin barrier.

However, due to external factors such as changes in the environment, air pollution and indoor heating, and internal factors caused by human aging, moisture in the stratum corneum decreases, resulting in a tendency to dry the skin and make the surface rough, even cracking. As a result, the need for skin moisturizers is increasing. To this end, as a skin moisturizer, NMF components and intercellular lipid components, which are constituents of the stratum corneum, and most commonly saccharides and polyhydric alcohols have been formulated and used in cosmetics as moisturizers. However, these general moisturizers have sufficient moisturizing effects and There is a disadvantage in that it does not provide a lasting moisturizing effect, and there is also a problem that stickiness occurs on the skin. In addition, in the summer when the humidity is very high, the skin is rather sticky by pulling moisture from the outside, and in winter, the humidity of the outside air is low, so the skin is dried by pulling moisture from the skin.

Various cosmetic compositions have tried to improve the dryness of the skin by using various moisturizing agents such as polyols and sugars. For example, natural moisturizing within the stratum corneum that suppresses moisture evaporating from the skin by strengthening the function of the skin barrier using skin components such as ceramide, fatty acid, cholesterol, etc., or easily binds with water molecules on the skin surface to maintain moisture in the stratum corneum. Moisturizing the skin by strengthening the skin's ability to retain moisture by using amino acids (natural moisturizing factor: NMF), lactate, sodium pyrrolidone carboxylic acid, and polyol, which has a strong ability to bind to moisture, sugar, and hyaluronic acid. To increase the effect.

Furthermore, in order to reduce skin irritation caused by external chemical substances, cosmetics using natural substances have been developed a lot. Natural ingredients have fewer side effects on the skin, and as consumers' demands for cosmetics using natural ingredients increase, the value of development as a cosmetic ingredient is further increasing.

The skin moisturizing effect of various mushroom extracts is known. For example, Korean Patent Laid-Open Publication No. 2011-0030187 discloses a cosmetic composition for moisturizing skin containing a mixed extract of blossom mushrooms, shinryeong mushrooms, and jiryeong. In addition, Korean Patent Laid-Open Publication No. 2013-0116641 discloses a composition for external application for skin containing a mushroom polysaccharide, and discloses that it has a wrinkle, elasticity improvement, skin immunity strengthening, and moisturizing effect.

Accordingly, the present inventors studied the possibility of application to cosmetics in various natural materials that were not known so far, and as a result of selecting the white western truffle (Tuber magnatum ) and measuring the moisturizing effect by preparing an extract therefrom It was found that this is very excellent, so that the efficacy as a cosmetic can be expected.

It is an object of the present invention to provide a cosmetic composition containing the extract of white western truffle (Tuber magnatum) showing a moisturizing effect.

In addition, an object of the present invention is to provide a cosmetic composition that exhibits an efficacy effect through a supercritical, ultrasonic, and fermented extract in order to maximize the efficacy of an extract of Tuber magnatum.

Accordingly, the object of the present invention as described above is achieved by a moisturizing cosmetic composition containing an extract of Tuber magnatum as an active ingredient.

In addition, preferably, the white western truffle extract is characterized in that it contains 0.001 to 30.0% by weight based on the total weight of the cosmetic composition.

In addition, preferably, the white pine truffle extract is (a) water, anhydrous or hydrated lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl An extraction method selected from the group consisting of a solvent extraction method using an extraction solvent selected from the group consisting of ether, dichloromethane, nucleic acids and mixtures thereof, (b) a supercritical extraction method, (c) an ultrasonic extraction method, and (d) a fermentation extraction method using microorganisms It characterized in that it is extracted by.

In addition, preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray. It is characterized by having a formulation selected from the group consisting of.

The white western truffle extract according to the present invention has excellent moisturizing effect (Experimental Example 1) and skin barrier improvement effect (Experimental Example 2).

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meaning as commonly understood by those of ordinary skill in the relevant fields of the present invention. In addition, although preferred methods or samples are described in the present specification, those similar or equivalent are included in the scope of the present invention.

The term “about” refers to 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, or 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, or relative to a reference amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length. It means an amount, level, value, number, frequency, percentage, dimension, size, amount, weight or length varying by 4, 3, 2 or 1%.

Throughout this specification, unless otherwise required by the context, the terms "comprise" and "comprising" include the steps or components presented, or groups of steps or components, but any other step or component, or It is to be understood that a step or group of components is not excluded.

The present invention provides a cosmetic composition for moisturizing the skin containing the white western pine (Tuber magnatum) extract as an active ingredient.

The white western truffle used in the present invention ( Tuber magnatum ) is made up of the central region of Europe, the northwest of Italy, the southeastern region of France, and the like. The season is from mid-October to the end of December, and can be found in the ground at a depth of about 3 cm near the roots of oak, hazel, and beech forests. The trend is to use'truffle dogs' for collection. The white western truffle is round and lumpy, and the outer part is hard, cracked, and creamy or yellowish brown.

The scientific name of the white western truffle is Tuber magnatum , and is called truffle mushroom and white truffle. The white truffle is very valuable. White truffles have a strong scent as if the smell of garlic and sulfur is mixed with the smell of dry soil. Especially, the scent is thicker than black truffles. White Western truffles are low in calories and rich in dietary fiber, so they are not burdened with weight control, and are foods that help prevent constipation. In addition, it contains minerals and vitamin C, so it is effective in relieving fatigue, preventing colds, and improving immunity.

In the present invention, the white western truffle extract means obtained by extracting from various organs or parts of the white western truffle, and preferably means obtained by extracting from mycelium.

In addition, the white western truffle extract of the present invention can be prepared according to a conventional method known in the art. For example, extraction is performed using (a) solvent extraction, (b) supercritical extraction by reduced pressure and high temperature by carbon dioxide, or (c) ultrasonic extraction.

In the present invention, preferably, a solvent extraction method using an extraction solvent was used.

In the present invention, the white pine truffle extract is a variety of extraction solvents, for example, water, anhydrous or hydrated lower alcohols having 1-4 carbon atoms (e.g., methanol, ethanol, propanol and butanol), propylene glycol, 1,3- Butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, nucleic acids, and can be obtained by using one or more extraction solvents selected from the group consisting of a mixture thereof, preferably ethanol , It is obtained using 70% (v/v) ethanol or water, and most preferably, it is obtained using 70% (v/v) ethanol.

On the other hand, it is obvious to those skilled in the art that the extract of the white pine trellis of the present invention can obtain an extract exhibiting substantially the same effect even when using not only the above-described extraction solvent but also other extraction solvents.

In addition, the white western truffle extract of the present invention includes not only the extract by the above-described extraction solvent, but also an extract that has undergone conventional purification and fermentation processes. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by extraction by ultrasonic wave, separation by using an ultrafiltration membrane having a certain molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or affinity) The active fraction obtained through various purification and extraction methods additionally carried out, such as an extract from a fermentation product using a natural state or various microorganisms) or separated by (produced for separation according to sex) are also included in the extract of the present invention.

In addition, the present invention provides a cosmetic composition, characterized in that the extract is obtained by concentrating under reduced pressure or freeze-drying a liquid product obtained by cooling, heating and filtration at room temperature, and an additional solvent.

The extraction method by the supercritical extraction method by the depressurization and high temperature by carbon dioxide refers to a supercritical fluid extraction method.In general, the supercritical fluid is a liquid which is obtained when the gas reaches the critical point under high temperature and high pressure conditions. It has gaseous properties and chemically has a polarity similar to that of non-polar solvents. Due to this property, supercritical fluids are used for the extraction of oil-soluble substances (J. Chromatogr. A. 1998;479:200-205).

Carbon dioxide becomes a supercritical fluid having both liquid and gaseous properties as the pressure and temperature reach a critical point due to the operation of the supercritical fluid device, and as a result, the solubility of the oil-soluble solute increases. When the supercritical carbon dioxide passes through an extraction container containing a certain amount of a sample, the fat-soluble substances contained in the sample are extracted and released into the supercritical carbon dioxide.

After extracting the fat-soluble material, if the sample remaining in the extraction container is passed through the supercritical carbon dioxide containing a small amount of co-solvent again, components that were not extracted with pure supercritical carbon dioxide can be extracted.

The supercritical fluid used in the supercritical extraction method of the present invention can effectively extract an active ingredient by using a supercritical carbon dioxide or a mixed fluid in which a co-solvent is additionally mixed with carbon dioxide.

As such co-solvent, one or a mixture of two or more selected from the group consisting of chloroform, olive oil, ethanol, methanol, water, ethyl acetate, nucleic acid, and diethyl ether may be used. Preferably, one or a mixture of two or more selected from the group consisting of olive oil, ethanol, methanol, and water may be used.

Most of the extracted samples contain carbon dioxide, and since carbon dioxide is volatilized into the air at room temperature, the extract obtained by the above method can be used as a cosmetic composition, and the co-solvent can be removed with a vacuum evaporator.

In addition, the ultrasonic extraction method is an extraction method using energy generated by ultrasonic vibration, and the ultrasonic wave can destroy the insoluble solvent contained in the sample in the water-soluble solvent. Since the kinetic energy of the reactant particles is increased, sufficient energy required for the reaction is obtained, and high pressure is induced by the impact effect of ultrasonic energy to increase the mixing effect of the material contained in the sample and the solvent, thereby increasing the extraction efficiency.

The extraction solvent that can be used in the ultrasonic extraction method may be one or a mixture of two or more selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, nucleic acid, and diethyl ether. The extracted sample may be vacuum filtered to recover the filtrate, then removed with a vacuum evaporator, and freeze-dried to obtain an extract through a conventional extract preparation method.

The white western truffle extract according to the present invention also includes an extract that has undergone a fermentation process, and the fermented extract of the white western truffle can be prepared as follows. After finely crushing white western truffles to about 100 to 500 mesh, 1 to 50 g/L of a conventional microorganism culture solution is added, and microorganisms such as yeast strains or lactic acid bacteria are added in an amount of 10,000 to 100,000 cfu/L. The culture temperature is 30~37℃ under the usual microbial culture conditions. The pH is 5-7, and incubation is performed for about 5-10 days under aerobic or usually anaerobic conditions. After that, it can be obtained through aging and filtration.

According to the present invention, the white western truffle extract is contained in an amount of 0.001 to 30.0% by weight, preferably, 0.01 to 20% by weight, and more preferably 0.1 to 10% by weight based on the total weight of the cosmetic composition. It is characterized by that. When the content of the extract is less than 0.001% by weight, the skin moisturizing effect does not appear, and when it exceeds 30.0% by weight, the degree of increase in the skin moisturizing effect due to the increase in content is insignificant. I am not even an enemy.

After removing unnecessary ingredients through a purification process in order to use the white western truffle extract prepared by a conventional method in a cosmetic formulation, a cosmetic composition exhibiting an efficacy may be included. The purification process may be any conventional purification process, and a liquid-liquid extraction method using a difference in solubility, a precipitation and a filter, and a chromatographic method using the size of polarity and molecular weight may be used. In addition, for example, after dissolving the white cypress extract in ethanol aqueous solution (50%, 70% solution, etc.) or other aqueous solution, using a column filled with HP-20 and sephadex columns, use the difference in polarity and molecular weight. After separating the water-soluble substance and the oil-soluble substance, the oil-soluble substance may be concentrated by TLC and freeze-dried to prepare a concentrate.

Ingredients included in the cosmetic composition of the present invention may include, as an active ingredient, ingredients commonly used in cosmetic compositions in addition to the above active ingredients, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and perfumes. It includes an adjuvant, and a carrier.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray may be formulated, but is not limited thereto. In more detail, it may be prepared in the form of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component. I can.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

In the case of cleansing the formulation of the present invention contains a surfactant, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid Amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters may be used.

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed off with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the cleansing formulation without surfactant is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Manufacturing example One: White western truffle Solvent extract preparation

300 g of the white Western truffle mycelium, which was cut and shaded, was extracted with reflux three times for 5 hours each for 5 hours with 70% (v/v) ethanol aqueous solution, cooled, and then filtered through Whatman #4 filter paper. After removing salts from the filtered extract using an ultrafiltration membrane, it was concentrated under reduced pressure at 50° C. and freeze-dried to obtain 6.7 g. Then, the dried white pine mushroom extract powder was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example 2: White western truffle Supercritical Fluid extract

300 g of the white western truffle mycelium, which was shredded and shaded, was extracted with a conventional supercritical extraction method (extracted using olive oil as a co-solvent in a supercritical state under a pressure of about 300 atm at a temperature of about 60°C). After removing the salt using an ultrafiltration membrane, it was concentrated under reduced pressure and freeze-dried to obtain 31.3 g of dried white western pine mushroom extract powder. This was dissolved in 50% 1,3-butylene glycol and used.

Manufacturing example 3: White western truffle Ultrasonic extract manufacturing

300g of the white Western truffle mycelium, which was shredded and shaded, was extracted using ultrasonic waves (extracted for about 2 hours at 30℃ at 25KHz using a supernonic ultrasonic device). After removing the salt using an ultrafiltration membrane, it was concentrated under reduced pressure and 7.1 g was obtained through freeze drying. The dried white pine mushroom extract powder was dissolved in 50% 1,3-butylene glycol and used.

Formulation example One: White western truffle Containing extract Cosmetic Composition

Cosmetics containing the white western truffle extract were prepared (Formulation Examples 1, 2, 3) each containing the white western truffle extract of Preparation Example 1, Preparation Example 2, and Preparation Example 3, for comparison of efficacy A cosmetic composition containing glycerin and 1,3-butylene glycol as a moisturizing agent (Comparative Formulation Example 1), a cosmetic composition comprising glycerin and sorbitol as a moisturizing agent (Comparative Formulation Example 2), extracts or moisturizers A cosmetic composition (Comparative Formulation Example 3) that does not contain all of them is shown in Table 1 (unit: content %).

ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 White Western truffle extract
(Production Example 1) 5.0 - - - - - White Western truffle extract
(Production Example 2) - 5.0 - - - - White Western truffle extract
(Production Example 3) - - 5.0 - - - glycerin - - - 5.0 5.0 - 1.3-butylene glycol - - - 5.0 - - Sorbitol - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.5 0.5 0.5 0.5 0.5 0.5 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 Floating paraffin 2.0 2.0 2.0 2.0 2.0 2.0 Trioctanoine 3.0 3.0 3.0 3.0 3.0 3.0 Polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 4.0 4.0 4.0 4.0 4.0 4.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount Purified water to 100 to 100 to 100 to 100 to 100 to 100

Experimental example One: White western truffle Moisturizing effect of extract

The clinical moisturizing effect and persistence for the cosmetic compositions of Formulation Examples 1, 2, and 3 were measured as follows. Through a questionnaire survey, 150 healthy adult men and women who feel dry skin were randomly formed into a group of 25, and the formulation for each group and Comparative Formulation Examples 1, 2 and 3 were each twice a day for 4 weeks. Each was applied to both faces. The moisturizing effect was evaluated by quantifying the increase rate (%) of skin electrical conductivity according to the amount of epidermal moisture using Corneometer CM 820 (Corage +Khazaka, Germany) for the improvement effect 2 weeks from the start of the actual use test and after the end of the test. The experimental results are shown in Table 2 below.

division Before use 2 weeks after use 4 weeks after use Formulation Example 1 24.20 40.22 47.07 Formulation Example 2 23.50 38.60 45.67 Formulation Example 3 22.64 40.85 48.67 Comparative Formulation Example 1 24.10 36.02 42.90 Comparative Formulation Example 2 24.95 37.42 41.62 Comparative Formulation Example 3 22.91 24.25 25.88

As shown in Table 2 above, compared to the cosmetic composition containing a chemical moisturizer (Comparative Formulation Examples 1 and 2) as a comparative formulation, the cosmetic composition containing the white pine truffle extract of the present invention (Formulation Examples 1, 2, 3) It was confirmed that the moisturizing effect was more excellent.

Experimental example 2: Skin barrier Improvement effect

This study was conducted on 25 healthy women indoors at 25°C, 45% relative humidity, and no air flow. Skin barrier damage was induced using a 1% aqueous solution of Sodium Lauryl Sulfate (SLS), and after applying the cosmetic composition of Formulation Examples 1, 2, and 3 to the upper arm of the arm, TEWAMETER TM210 (C+K eletronic) GmhH. Germany) was used to measure the amount of transdermal moisture loss. That is, by measuring the amount of change in TEWL (Transepidermal Water Loss) value according to time change, the skin barrier recovery rate was confirmed. The experimental results are shown in Table 3 below.

division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 52.7 65.8 83.2 97.3 Formulation Example 2 53.2 65.8 87.3 98.1 Formulation Example 3 52.6 64.3 84.6 98.7 Comparative Formulation Example 3 42.1 58.5 65.5 73.2

As shown in Table 3, it can be seen that the amount of transdermal moisture loss was improved in Formulation Examples 1, 2, and 3 containing the white pine truffle extract compared to Comparative Formulation Example 3 not containing the white pine truffle extract.

Experimental example 3: safety test

A human skin patch test was conducted to check whether the cosmetic compositions of Formulation Examples 1, 2, and 3 were irritated to the skin. In the past, 20 women in their 20s to 30s who have never had hypersensitivity to skin irritation and do not have skin allergies or skin allergies were tested. I did.

First, the test area was wiped with 70% ethanol and then dried. The prepared test substance was dropped into a finn chamber (100X10, EPITEST), and then hermetically patched on the back of the test subject. The patch was applied for 24 hours, and the presence or absence of erythema and edema at the test site was observed using a magnifying glass (8MC-150, DAZOR) after 1 hour and 24 hours after removing the patch.

The skin reaction was determined according to the regulations (Table 4) of the International Contact Dermatitis Research Group (ICDRG), and the average skin reactivity was calculated according to Equation 1 below, and the results are shown in Table 5.

sign score Evaluation standard - 0 No reaction ± 0.5 Faint or mild erythema + One Erythema, swelling, and papules that are clearly bordered but weak ++ 2 Pronounced erythema, papules, and vesicles +++ 3 Severe erythema and blisters, crust formation

[Equation 1]

division 24 hours 48 hours Average skin reactivity
(n=20) ± + ++ ± + ++ Formulation Example 1 One - - - - - 0.14 Formulation Example 2 - - - - - - 0.00 Formulation Example 3 One - - - - - 0.14 Comparative Formulation Example 3 2 - - - - - 0.28

As shown in Table 5, it was confirmed that the cosmetic compositions of Formulation Examples 1, 2, and 3 did not cause skin irritation, and it was found that there was no abnormality in the safety of the cosmetic formulation to which the white pine pine tree extract was added.

As the specific parts of the present invention have been described in detail above, it is clear that these specific techniques are only preferred embodiments for those of ordinary skill in the art, and the scope of the present invention is not limited thereto. Therefore, it will be said that the practical scope of the present invention is defined by the appended claims and their equivalents.

Claims (4)

A cosmetic composition for moisturizing the skin containing the white western pine tree (Tuber magnatum) extract as an active ingredient. The cosmetic composition for moisturizing skin according to claim 1, wherein the white western pine tree extract is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. The method of claim 1, wherein the white pine truffle extract is (a) water, anhydrous or hydrated lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl A solvent extraction method using an extraction solvent selected from the group consisting of ether, dichloromethane, nucleic acids, and mixtures thereof, (b) supercritical extraction, (c) ultrasonic extraction, and (d) fermentation extraction using microorganisms. Skin moisturizing cosmetic composition extracted by. The method of claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray. A cosmetic composition for moisturizing skin, characterized in that it has a formulation selected from the group consisting of.